CN103719151B - One cultivates peanut seed dressing and preparation method thereof and application - Google Patents
One cultivates peanut seed dressing and preparation method thereof and application Download PDFInfo
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- CN103719151B CN103719151B CN201310714836.3A CN201310714836A CN103719151B CN 103719151 B CN103719151 B CN 103719151B CN 201310714836 A CN201310714836 A CN 201310714836A CN 103719151 B CN103719151 B CN 103719151B
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- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 100
- 235000020232 peanut Nutrition 0.000 title claims abstract description 100
- 241001553178 Arachis glabrata Species 0.000 title claims abstract 17
- 238000002360 preparation method Methods 0.000 title abstract description 6
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- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 15
- 239000004571 lime Substances 0.000 claims abstract description 15
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- 239000005648 plant growth regulator Substances 0.000 abstract description 8
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses one to cultivate peanut seed dressing and preparation method thereof and application.Peanut seed dressing comprises sodium silicate, nitrate of lime and plant growth regulator, and plant growth regulator is one or more in indolebutyric acid, methyl α-naphthyl acetate, study on brassinolide.The present invention has effectively alleviates peanut Acid-Al stress ability, has short root effect in strong sprout preferably; Its production technology is simple, and using method is easy, is easy to apply.
Description
Technical field
The present invention relates to agriculture field, particularly a kind of peanut seed dressing being applicable to southern area and preparation method thereof and application.
Background technology
South is red-yellow soil area, in recent years along with the increase increasingly of the fast development of industry and a large amount of use of agrochemical and acid rain, the acidizing degree of soil increases thereupon, and in soil, the content of activated aluminum and stripping quantity also increase considerably, very large to the aluminium poison harm of crop.Therefore, malicious having become in China's acid ground of aluminium limits one of plant growth and the principal element causing crop failure.Since Hartwell and Pember Late Cambrian in 1918 aluminium to the toxic action of plant since, to the research of aluminium poison close to 100 years, but its mechanism of action it be unclear that so far, and its main cause is that aluminium has very complicated chemical property.In acid ground solution (pH<5), aluminium is mainly with Al
3+form exists; With the rising of pH, form Al (OH) gradually
2+, Al (OH)
2 +; In neutral soil solution (pH=7), aluminium mainly exists with insoluble silicates and oxides form, mainly Al (OH)
3precipitation; When to be subject in highly basic and time, the existence form that toxicity is larger can be produced---hydroxy aluminum Al (OH)
4 -.The toxicity size of different shape aluminium is different because of vegetation type.For dicotyledon as buckwheat, soybean, radish, rape etc., Al (OH)
2+, Al (OH)
2 +toxic compare Al
3+much better than, monocotyledon is if barley, corn, wheat, rye etc. are then to Al
3+more responsive.Element silicon is element the abundantest beyond deoxygenation in the earth's crust, and it usually coexists in the soil solution with aluminium ion.Meanwhile, the beneficial element that element silicon grows as higher plant, plays an important role in the resistance of biotic (sick worm) and abiotic stress (heavy metal, salt damage, drought stress etc.) improving plant.Silicon in somatomedin can alleviate the toxic action of aluminium to crops such as Chinese sorghum, chinese sorghum, barley, corn, paddy rice, buckwheats effectively.But the research of coercing about silicon alleviation plant aluminum is at present reported less.
Peanut is the important oil crop of China and export crop, and national cultivated area reaches 5,000,000 hectares, and wherein southern red yellow earth area accounts for the whole nation about 30%.Because red-yellow soil major part is in acid or strong acid reaction, exchangeable aluminum ion accounts for 20% ~ 80% of cation exchange capacity, and Development of Peanut is seriously subject to the harm of aluminium poison, this be this region for a long time peanut per unit area yield always lower than the major reason of average national level.At present, the peanut seed dressing of application in production is mainly for soil-borne disease, leaf portion disease and insect pest, and these all belong to the biotic of peanut, less about the peanut seed dressing kind alleviating abiotic stress (as aluminium toxicity, salt stress etc.).
Summary of the invention
The object of the invention is to for the serious problem of southern red yellow earth aluminium poison harm, the peanut seed dressing being applicable to southern area providing a kind of and can improve Peanut Root System vigor, improve root growth and development, promote Nutrient Absorption and utilization.
Another object of the present invention is to the preparation method that above-mentioned peanut seed dressing is provided.
Another object of the present invention is the application providing above-mentioned peanut seed dressing.
Object of the present invention is achieved through the following technical solutions:
One cultivates peanut seed dressing, comprises sodium silicate (Na
2siO
3), nitrate of lime (Ca
2nO
34H
2and plant growth regulator O); Described plant growth regulator is one or more in indolebutyric acid (IBA), methyl α-naphthyl acetate (NAA), study on brassinolide (2a, 3a, 22s, 23s-tetrahydroxy-24R-ethyl-β-Gao-7-oxa--5a-courage steroid-6-ketone, BR), is preferably indolebutyric acid.
Also comprise auxiliary agent and water in peanut seed dressing, auxiliary agent can be solvent, emulsifier, dispersant, wetting agent, antifreezing agent, thickener, defoamer etc.Described solvent preferred alcohol, dimethylbenzene; Described emulsifier preferred agriculture breast 500(calcium dodecyl benzene sulfonate), Tween 80 (polysorbate80); The sodium salt of described dispersant preferred MorwetD-425(alkylnaphthalene sulfonate condensation polymer); The preferred MorwetIP(isopropyl naphthalene sulfonate of described wetting agent); The preferred ethylene glycol of described antifreezing agent; The preferred xanthans of described thickener; The preferred import organosilicon of described defoamer.Above-mentioned auxiliary agent is commercial goods.
The consumption of described sodium silicate is 3 ~ 6 weight portions, and nitrate of lime is 20 ~ 40 weight portions, and plant growth regulator is 0.5 ~ 1.2 weight portion.
The concentration of described sodium silicate is preferably 0.1 ~ 0.6gL
-1, the concentration of nitrate of lime is preferably 2.0 ~ 4.0gL
-1, the concentration of plant growth regulator is preferably 0.1 ~ 1.0gL
-1.
The preparation method of above-mentioned peanut seed dressing comprises the steps: sodium silicate, nitrate of lime, plant biotechnology regulator, auxiliary agent, water mixing, after pre-dispersed with high shearing mixing emulsor, be poured into sand mill, after connecting condensed water, open sand mill, grind 2 ~ 5 hours, filter the peanut seed dressing of obtained different dosage form.
The formulation of peanut seed dressing can be aqueous emulsion, suspending agent, emulsifiable concentrate or microemulsion.
The application of described peanut seed dressing comprises the steps: the peanut seed dressing of thin up to pour into be equipped with in the container of peanut seed, turns evenly, is dried in the air by peanut seed 1 ~ 2 hour, can sow.
Concentration after the thin up of peanut seed dressing is preferably 0.5 ~ 1.2gL
-1, preferred 0.9gL
-1.
Principle of the present invention is: calcium ion is as second messenger most important in plant cell, and involved in plant is to the transduction of many adverse circumstance signals and adjustment.Under Acid-Al stress condition, calcium ion is as Al
3+main competitive cation, increase the competition of Root Absorption site and activated aluminum; Calcium ion is as courier, and adverse circumstance signal in regulating plant body, improves tonoplast H
+-ATP enzyme and Ca
2+-atpase activity, promotes plant normal growth.Plant growth regulator (indolebutyric acid, methyl α-naphthyl acetate, study on brassinolide etc.) can improve the activity of seed vitality and enzyme, can also improve its water absorbing capacity, promotes root growth and development.
The present invention compared with prior art tool has the following advantages and effect:
(1) the present invention with sodium silicate and nitrate of lime for active ingredient, utilize the resistance of element silicon and the competitiveness of calcium ion and courier's characteristic, after peanut absorbs silicon and calcium, improve Peanut Root System vigor and resistance, alleviate soil aluminium and coerce, promote that Peanut Root System is to the absorption of the elements such as N, P, K, Ca, Mg and utilization, prick under promoting root system, thus promoting that Peanut Root System is strong, the aluminum-resistant improving peanut coerces ability, ensures that the normal growth of peanut is grown.
(2) owing to adding plant growth regulator, seed vitality, the activity of enzyme and improving activity of root system can be improved, promote growing of Peanut Root System.
(3) peanut seed dressing of the present invention has effectively alleviation peanut Acid-Al stress ability, has short root effect in strong sprout preferably; Its production technology is simple, and using method is easy, is easy to apply.
Accompanying drawing explanation
Fig. 1 peanut seed dressing is on the impact of improving activity of root system.
Fig. 2 peanut seed dressing is on the impact of root system peroxidase activity.
Fig. 3 peanut seed dressing is on the impact of root system mda content.
Fig. 4 peanut seed dressing is on the impact of root system proline content.
Fig. 5 peanut seed dressing is on the impact of root system aluminium element content.
Embodiment
Below in conjunction with embodiment, further detailed description is done to the present invention, but embodiments of the present invention are not limited thereto." part " in embodiment namely represents " weight portion ", and except specified otherwise, other is this area normal experiment reagent and method.
Embodiment 1
4.5 parts of sodium silicate, 34.7 parts of nitrate of lime, 0.8 part of methyl α-naphthyl acetate, 4.0 parts of ethanol, 6.0 parts of Tween 80s, 3.0 parts of ethylene glycol, 0.2 part of xanthans, 0.2 part of import organosilicon, 46.6 parts of water, above-mentioned substance is mixed, after pre-dispersed with high shearing mixing emulsor, is poured into sand mill, after connecting condensed water, open sand mill, grind 2 hours, filter the peanut seed dressing of obtained water and milk shape.
Embodiment 2
4.8 parts of sodium silicate, 34.4 parts of nitrate of lime, 0.8 part of indolebutyric acid, 3.0 parts of MorwetD-425,2.0 parts of MorwetIP, 5.0 ethylene glycol, 0.2 part of xanthans, 0.2 part of import organosilicon, after 49.6 parts of water mixing, obtains peanut seed dressing (suspension).
Embodiment 3
5.0 parts of sodium silicate, 36.2 parts of nitrate of lime, 0.8 part of methyl α-naphthyl acetate, 3.0 parts of MorwetD-425,2.0 parts of MorwetIP, 5.0 parts of ethylene glycol, 0.2 part of xanthans, 0.2 part of import organosilicon, after 47.6 parts of water mixing, obtains peanut seed dressing (suspension).
Embodiment 4
5.2 parts of sodium silicate, 36.0 parts of nitrate of lime, 0.8 part of indolebutyric acid, 3.0 parts of MorwetD-425,2.0 parts of MorwetIP, 51.6 parts of dimethylbenzene, 1.0 parts of agriculture breasts, 500,0.2 part of xanthans, after 0.2 part of organosilicon mixing, obtains peanut seed dressing (creamy).
Embodiment 5
5.5 parts of sodium silicate, 34.0 parts of nitrate of lime, 0.3 part of indolebutyric acid, 0.2 part of study on brassinolide, 50.6 parts of ethanol, 6.0 parts of Tween 80s, 3.0 parts of ethylene glycol, 0.2 part of xanthans, after 0.2 part of import organosilicon mixing, obtains peanut seed dressing (microemulsion).
Embodiment 6
5.5 parts of sodium silicate, 36.0 parts of nitrate of lime, 0.5 part of study on brassinolide, 48.6 parts of ethanol, 6.0 parts of Tween 80s, 3.0 parts of ethylene glycol, 0.2 part of xanthans, after 0.2 part of import organosilicon mixing, obtains peanut seed dressing (microemulsion).
Test case (one)
Laboratory test is carried out in laboratory, and test kind is Guangdong oil No. 7 peanut seeds.Select in the same size, mellow and full full peanut seed, the peanut seed dressing (water and milk shape) prepared with above-described embodiment processes.Peanut seed dressing is converted clear water appropriate (being about 1:120 with pesticide-seeds ratio) and is stirred, peanut (foundation South China peanut seeding amount, Guangdong oil No. 7 180 ~ 220kghm after weighing
-2) spread out on a plastic film or in basin, the peanut seed dressing after thin up is sprinkled upon on peanut seed and turns evenly, spread out in the cool and dry latter 2 hours, can sow.Be seeded in clean sand, 26 DEG C of vernalization 4 days, removes seed coat, is transplanted in improvement Hoagland nutrient solution and cultivates, within every 2 days, change one time of nutrition liquid.After 7 days, carry out Acid-Al stress (AlCl
36H
2o, 40.0mgL
-1) process.Test establishes 6 chemicals treatment, that is: CK(blank, 0gL
-1), A(0.3gL
-1), B(0.6gL
-1), C(0.9gL
-1), D(1.2gL
-1) and E(1.5gL
-1), wherein CK is reference examples, does not add peanut seed dressing; A ~ E is the peanut seed dressing adding variable concentrations.
(1) biomass analysis
As shown in Table 1, Acid-Al stress process significantly reduces Roots of Peanut length, root surface area, average root diameter, root fresh weight and dry weight.Compared with reference examples, the process of peanut seed dressing significantly improves peanut plant root length, root surface area, average diameter, root fresh weight and dry weight, wherein with C process (0.9gL
-1peanut seed dressing) effect is best, and peanut plant root length, root surface area, average diameter, root fresh weight and dry weight add 27.3%, 26.0%, 49.0%, 35.4% and 95.6% respectively.
Under table 1 Acid-Al stress condition, peanut seed dressing is on the impact of Peanut Root System form and biomass
Note: 1, CK: blank; A:0.3gL
-1; B:0.6gL
-1; C:0.9gL
-1; D:1.2gL
-1; E:1.5gL
-1.
2, the different lowercase of same column represents that 5% level difference is remarkable.
(2) improving activity of root system analysis
Improving activity of root system reflection plant root to the absorbing capacity of moisture and nutriment, and has important function to the whole strain function of maintenance.As seen from Figure 1, reference examples (Acid-Al stress) process significantly reduces Peanut Root System vigor.Compared with reference examples, the process of peanut seed dressing significantly improves the improving activity of root system of plant.
(3) peroxidase activity analysis
Self-protection system in peroxidase (POD) active reaction plant body, peroxidase to play a part in purged body too much oxyradical, and Cell protection film preserves from.As shown in Figure 2, compared with reference examples, the peroxidase content of peanut seed dressing process peanut plant significantly improves, and this shows that peanut seed dressing is coerced raising plant aluminum-resistant and serves certain effect.
(4) root system mda content is analyzed
In plant corpus, excess activated oxygen radical can cause peroxidation of membrane lipids, and MAD is one of primary product of Lipid peroxidation metabolism, and its accumulation is the performance of toxicity of biological active oxygen effect, is one of the index of Lipid peroxidation metabolism.Compared with reference examples, the plant mda content of peanut seed dressing process significantly reduces, with C process (0.9gL
-1seed dressing) effect is best, reduces 33.0%(Fig. 3).This shows that plant is owing to being subject to the effect of aluminium toxicity, and mda content increases, and cell membrane system defines injury to a certain degree.The process of peanut seed dressing, plant reduces the injury of Acid-Al stress to a certain extent, plays the effect of protection plant.
(5) root system proline content is analyzed
In plant corpus, free proline content has reacted plant to a certain extent and suffers the situation of adverse circumstance and plant to the resistivity of adverse circumstance.Compared with reference examples, the proline content of peanut seed dressing process Peanut Root System significantly reduces (Fig. 4).This shows that peanut is owing to being subject to the effect of aluminium toxicity, and proline content increases, and strengthening roots agent alleviates peanut aluminium poison.
(6) plant root aluminium content analysis
Under Acid-Al stress, the root system of some aluminum-tolerant plant can secrete organic acid.These organic acids and aluminium ion form organic chelate, and toxicity is lower, thus reach the effect reducing aluminium ion concentration.As seen from Figure 5, compared with reference examples, peanut seed dressing process plant aluminium content significantly reduces.This shows that peanut seed dressing produces regulation and control to a certain degree to the absorption of plant aluminium.
Test case (two)
Field trial is carried out in proving ground, and experimental cultivar is No. 7, Guangdong oil and blue and white No. 7 peanut seeds (commercial).Before peanut seeding, use peanut seed dressing (suspension) effective dose of the present embodiment to be 1.0g/L, be watered 200 ~ 250mL and stir, by after weighing peanut (according to peanut in South China's seeding quantity, Guangdong oil No. 7 180 ~ 220kghm
-2, blue and white No. 7 380 ~ 440kghm
-2) spread out on a plastic film or in basin, the peanut seed dressing prepared is sprinkled upon on peanut seed and turns evenly, spread out after drying in the cool, can sow.Each kind establishes 2 process, that is: reference examples (0gL
-1) and peanut seed dressing process (1.0gL
-1), each process repetition 3 times, plot area 20m
2.The peanut growth phase adopts Routine Management, and gather in the crops after three months, 1m is chosen in each community
2, for surveying product.
As shown in Table 2, the process of peanut seed dressing all has significant effect of increasing production to two peanut varieties.Compared with control treatment, Guangdong oil No. 7 seed dressing process output increase by 10.5%; Blue and white No. 7 kind seed dressing process output increase by 9.8%.
Table 2 peanut seed dressing is on the impact of peanut yield
Note: the different lowercase of same column represents that 5% level difference is remarkable.
Interpretation of result
In sum, after the process of peanut seed dressing, each physical signs of peanut plant all reflects it and has the ability resisting aluminium toxicity to a certain degree.Field result shows, peanut seed dressing has good effect of increasing production.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1. an application of cultivating peanut seed dressing, is characterized in that comprising the steps: the peanut seed dressing of thin up to pour into be equipped with in the container of peanut seed, turns evenly, is dried in the air by peanut seed 1 ~ 2 hour, can sow; Described peanut seed dressing comprises sodium silicate, nitrate of lime and methyl α-naphthyl acetate, and wherein the consumption of sodium silicate is 3 ~ 6 weight portions, and nitrate of lime is 20 ~ 40 weight portions, and methyl α-naphthyl acetate is 0.5 ~ 1.2 weight portion.
2. the application of peanut seed dressing according to claim 1, is characterized in that: in described peanut seed dressing, and the concentration of sodium silicate is 0.1 ~ 0.6gL
-1, the concentration of nitrate of lime is 2.0 ~ 4.0gL
-1, the concentration of methyl α-naphthyl acetate is 0.1 ~ 1.0gL
-1.
3. the application of peanut seed dressing according to claim 1, is characterized in that: the concentration after the thin up of peanut seed dressing is 0.5 ~ 1.2gL
-1.
4. the application of peanut seed dressing according to claim 3, is characterized in that: the concentration after the thin up of peanut seed dressing is 0.9gL
-1.
5. the application of peanut seed dressing according to claim 1, is characterized in that: also comprise one or more in emulsifier, dispersant, wetting agent, antifreezing agent, thickener, defoamer, water in peanut seed dressing.
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| CN103964973B (en) * | 2014-05-19 | 2016-01-06 | 山东省农业科学院生物技术研究中心 | The special seed dressing of peanut |
| CN112841210A (en) * | 2021-01-15 | 2021-05-28 | 上海生农生化制品股份有限公司 | Dispersing wetting agent composition |
| CN115245175B (en) * | 2022-08-30 | 2023-10-10 | 淮阴师范学院 | Seed treating agent and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153423A (en) * | 2011-03-26 | 2011-08-17 | 邓云村 | Rice full-nutrition active acid-regulated fertilizer |
| CN103013527A (en) * | 2011-09-20 | 2013-04-03 | 张科全 | Acid soil pH value improvement regulator and preparation method thereof |
| CN103238628A (en) * | 2013-04-09 | 2013-08-14 | 华南农业大学 | Agent for controlling excessive vegetative growth and strengthening seedlings of peanut and preparation method and application of agent |
| CN103267667A (en) * | 2013-05-08 | 2013-08-28 | 华南农业大学 | Method for rapidly detecting aluminum toxicity of root of crop under soil cultivation condition |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102153423A (en) * | 2011-03-26 | 2011-08-17 | 邓云村 | Rice full-nutrition active acid-regulated fertilizer |
| CN103013527A (en) * | 2011-09-20 | 2013-04-03 | 张科全 | Acid soil pH value improvement regulator and preparation method thereof |
| CN103238628A (en) * | 2013-04-09 | 2013-08-14 | 华南农业大学 | Agent for controlling excessive vegetative growth and strengthening seedlings of peanut and preparation method and application of agent |
| CN103267667A (en) * | 2013-05-08 | 2013-08-28 | 华南农业大学 | Method for rapidly detecting aluminum toxicity of root of crop under soil cultivation condition |
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