CN103655438A - Biological fermentation night cream and preparation method thereof - Google Patents
Biological fermentation night cream and preparation method thereof Download PDFInfo
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- CN103655438A CN103655438A CN201310199696.0A CN201310199696A CN103655438A CN 103655438 A CN103655438 A CN 103655438A CN 201310199696 A CN201310199696 A CN 201310199696A CN 103655438 A CN103655438 A CN 103655438A
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Abstract
The invention relates to a biological fermentation night cream and a preparation method thereof. The method comprises the following steps: adding cetostearyl alcohol, natural jojoba oil, squalane and vitamin E into an oil-phase pan, heating to 75-80 DEG C, and sufficiently stirring until dissolving uniformly; pouring all water into a water-phase pan, slowly pouring glycerol and maltooligosaccharide-base glucoside, scattering hyaluronic acid while heating to 75-80 DEG C, and keeping the temperature for half an hour after all the raw materials are dissolved; carrying out vacuum filtration on the oil phase in the oil-phase pan into an emulsifying pan, starting stirring, carrying out vacuum filtration on the water phase in the water-phase pan into the emulsifying pan while slowly homogenizing, and quickly homogenizing for 3 minutes when all the materials are sucked into the emulsifying pan; and stopping homogenizing, sequentially adding essence, aloe extract and composite fermented amino acid complete-effect nutrient solution, uniformly stirring, and cooling while vacuumizing in the whole process. Compared with the prior art, the product provided by the invention has the advantages of mild properties, high active components and moist texture, and has the functions of moisturization, repair, whitening, aging resistance, tightening and nourishing.
Description
Technical field
The present invention relates to a kind of washing and skin care articles for use, especially relate to a kind of biofermentation late frost and preparation method thereof.
Background technology
Late frost is conventional skincare product, and for the skin protection at night, of a great variety on market, effect is separately also uneven, and quality discrepancy is larger.
Substantially this all series products all adopts industrial chemicals synthetic, though powerful, its side effect is also very obvious.This biofermentation late frost adopts biofermentation technique, comprises the needed whole nutrition of hair quality, takes the course of its own at home.These properties of product are gentle, and active component is high, and quality is also more moist, and there is moisturizing, maintenance, whitening, defying age, compact, nutrition moistens.
Summary of the invention
Object of the present invention is exactly to provide a kind of performance gentle in order to overcome the defect of above-mentioned prior art existence, active component is high, quality is also more moist, and there is moisturizing, maintenance, whitening, defying age, compact, biofermentation late frost that nutrition is moist and preparation method thereof.
Object of the present invention can be achieved through the following technical solutions: a kind of biofermentation late frost, it is characterized in that, and comprise the component of following weight percentage:
Composite fermentation aminoacid is imitated nutritional solution 10%～15% entirely;
Oligomeric maltose base glucoside 3%～3.5%;
Natural Jojoba oil 5.5%～6%;
Glycerol 4%～6%;
Cetearyl alcohol 2.5%～3.5%;
Squalane 4%～6%;
Vitamin E 0.25%～0.35%;
Hyaluronic acid 0.04%～0.06%;
Aloe extraction solution 2.0%～2.5%;
Essence 0.2%～0.3%;
Water surplus.
Described composite fermentation aminoacid is entirely imitated nutritional solution and is made by the following method: raw material is placed in to pottery static fermentation 21～30 days, then the product obtaining is taken out and filtered, collect filtrate and control temperature and be 100～110 ℃ and carry out high temperature sterilize, finally be cooled to room temperature, obtain composite fermentation aminoacid and entirely imitate nutritional solution.
Described raw material comprises the component of following weight percent content: lactic acid bacterial liquid 0.5～1.0%, pink yeast liquid 0.5～1.0%, Phanerochaete chrysosporium bacterium liquid 0.5～1.0%, Bacillus licheniformis liquid 0.5～1.0%, Bacillus pumilus bacterium liquid 0.5～1.0%, maltose candidiasis liquid 0.5～1.0%, long shoot trichoderma liquid 0.5～1.0%, shatian pomelo 0.4～0.6%, Fructus Mali pumilae 0.4～0.6%, Fructus Musae 0.4～0.6%, Lac regis apis 0.2～0.3%, extraordinary Semen Glycines 1.0～1.2%, Radix Ginseng 0.5～0.9%, Ganoderma 0.5～0.9%, lavandula angustifolia 1.0～1.2%, rice distiller grain 20～25%, surplus is pure water.
Described lactic acid bacterial liquid prepares by the following method:
(1) prepare lactobacillus culture medium raw material, comprise following component and weight percent content: milk casein peptone 8～12%, peptone 8～12%, yeast extract 4～6%, glucose 4～6%, tween 80 0.8～1.2%, dipotassium hydrogen phosphate 1.8～2.5%, sodium acetate 4～6%, citric acid diamidogen 1.8～2.5%, magnesium sulfate 0.1～0.3%, manganese sulfate 0.04～0.06%, surplus are pure water;
(2) preparation of fluid medium: press lactobacillus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the lactobacillus inoculation of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the sterilized fluid medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain lactic acid bacterial liquid.
Described pink yeast liquid prepares by the following method:
(1) prepare pink Yeast Cultivation based raw material, comprise following component and weight percent content: peptone 4.1～6%, glucose 8～12%, yeast extract 2～4%, surplus are beerwort;
(2) preparation of fluid medium: by pink Yeast Cultivation based formulas preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the pink barms of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain pink yeast liquid.
Described Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9～2.0%, KH
2pO
40.25～0.30%, MgSO
47H
2o0.14～0.15%, vitaminB10 .01%, agar 1.4～1.5%, surplus are Rhizoma Solani tuber osi extracting solution;
(2) preparation of fluid medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Phanerochaete chrysosporium strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Phanerochaete chrysosporium bacterium liquid.
Described Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: the NaOH solution that wheat bran 4.5～4.7%, soybean cake powder 2.7～2.85%, agar 1.8～1.85%, surplus are 0.2wt%, after wheat bran, soybean cake powder are mixed, add 0.2wt%NaOH solution and boil 1h, with HCl, neutralizing pH is 7, then adds agar fusing;
(2) preparation of fluid medium: the sample that step (1) is obtained is at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus licheniformis strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus licheniformis liquid.
Described Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare Bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4～0.6%, beef leaching thing 0.25～0.35%, NaCl0.4～0.6%, agar 1.4～1.5%, surplus are distilled water;
(2) preparation of fluid medium: by Bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus pumilus strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus pumilus bacterium liquid.
Described maltose candidiasis liquid prepares by the following method:
(1) prepare maltose candida mycoderma culture medium raw material, comprise following component and weight percent content: agar 1.4～1.6%; 5 ° of beerworts 98.4～98.6%;
(2) preparation of fluid medium: press maltose candida mycoderma culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the maltose candida strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain maltose candidiasis liquid;
Described long shoot trichoderma liquid prepares by the following method:
(1) prepare long shoot Trichoderma spp. culture medium raw material, comprise following component and weight percent content: agar 1.4～1.6%; 5 ° of beerworts 98.4～98.6%;
(2) preparation of fluid medium: by long shoot Trichoderma spp. culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the long shoot Trichoderma spp. strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain long shoot trichoderma liquid;
Described Fructus Mali pumilae is the Fructus Mali pumilae of selecting the Qinling Mountains or Dalian,
Described Fructus Musae is selected the Fructus Musae in Yunnan,
Described extraordinary Semen Glycines is selected the extraordinary Semen Glycines into Shaanxi or Heilungkiang.
A preparation method for biofermentation late frost, is characterized in that, the method comprises the following steps:
(1) take by weight percentage: composite fermentation aminoacid is imitated nutritional solution 10%～15% entirely; Oligomeric maltose base glucoside 3%～3.5%; Natural Jojoba oil 5.5%～6%; Glycerol 4%～6%; Cetearyl alcohol 2.5%～3.5%; Squalane 4%～6%; Vitamin E 0.25%～0.35%; Hyaluronic acid 0.04%～0.06%; Aloe extraction solution 2.0%～2.5%; Essence 0.2%～0.3%; Water surplus;
(2) first cetearyl alcohol, natural Jojoba oil, squalane, vitamin E are dropped into oil phase pot and be heated to 75～80 ℃, stir until dissolved to homogeneous, 50 revs/min of mixing speeds;
(3) whole water is poured in water pot, then slowly pour glycerol, Oligomeric maltose base glucoside into, after stirring and being uniformly dispersed for 10 minutes, be sprinkled into hyaluronic acid, after stirring and being uniformly dispersed for 30 minutes, heating, is warmed up to 75～80 ℃ simultaneously, mixing speed is 50 revs/min, and raw material is incubated half an hour after all dissolving;
(4) first by the oil phase sucking filtration in oil phase pot to emulsifying pot, open to stir, mixing speed is 80 revs/min, at a slow speed when homogenizing again by the water sucking filtration in water pot to emulsifying pot, temperature keeps 75 ℃, after expecting the whole suction of body, fast homogeneous is 3 minutes; Homogenizing mixing speed is 2500 revs/min;
(5) stop homogenizing, cool to below 50 ℃, add successively essence, Aloe extraction solution, composite fermentation aminoacid entirely to imitate nutritional solution and stir, all add rear continuation to stir 30 minutes, in the time of cooling, omnidistance evacuation, stirs 50 revs/min;
(6) stir and be cooled to 38 ℃, stop stirring, check, discharging, obtain biofermentation late frost product.
The main component of biofermentation late frost of the present invention is that composite fermentation aminoacid is imitated nutritional solution entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligosaccharide, the nutrient substance of more than the 40 kind of needed by human such as the mineral such as soybean phospholipid, soybean saponin, VitC, VitE, B group vitamin, zinc, selenium, phosphorus, ferrum, calcium and trace element, beta-carotene, thiamine, citric acid.
The composite fermentation aminoacid that described biofermentation late frost adopts is entirely imitated nutritional solution, essence etc. and is adopted natural biology extract, does not add hormonal substance.
Wherein:
Hyaluronic acid is current confessed best moisturizing composition, can improve skin-nourishing metabolism, make that skin is tender, smooth, wrinkle removing, increase elasticity, prevent aging, and in moisturizing, be again good Percutaneous absorption enhancer
Oligomeric maltose base glucoside is the carbohydrate syrup being born by starch derivatives, contains various saccharides, can protect Skin Cell and moisturizing, and product is fine quality, to skin without any stimulation.
Natural Jojoba oil is pure natural transparency liquid, contains and enriches vitamin, has the effect of nourishing softening skin, easily by skin, is absorbed.
Cetearyl alcohol is as the substrate of this biofermentation late frost.Squalane extracts from deepwater shark liver, and skin is had to good affinity.
Vitamin E is a kind of fatsoluble vitamin, is that human normal growth institute is necessary.
Superfine titanium white plays the effect of beautifying whitening.
Glycerol is pleasantly sweet, is water white transparency viscous liquid, and moisturizing effect is very good, with the soft skin of rear water water profit profit.
Aloe extraction solution is that natural moisturizing promotes fiber polymeric protein to fall into NMF with lock water, effect mechanism, thereby increases nature moisturizing factor, immediately increases water content, by slight filming function, reduces percutaneous water loss.
Essence adopts plant extract essence, adds as required variety classes, as Coconut milk, Cymbidium ensifolium (L.) Sw. etc.
Compared with prior art, biofermentation late frost of the present invention adopts biofermentation technique, comprises the needed whole nutrition of hair quality, takes the course of its own at home.These properties of product are gentle, and active component is high, and quality is also more moist, and there is moisturizing, maintenance, whitening, defying age, compact, nutrition moistens.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A preparation method for biofermentation late frost, the method comprises the following steps:
(1) take by weight percentage: composite fermentation aminoacid is imitated nutritional solution 12% entirely; Oligomeric maltose base glucoside 3.2%; Natural Jojoba oil 5.8%; Glycerol 5%; Cetearyl alcohol 3.0%; Squalane 5%; Vitamin E 0.30%; Hyaluronic acid 0.05%; Aloe extraction solution 2.3%; Essence 0.25%; Water surplus;
(2) first cetearyl alcohol, natural Jojoba oil, squalane, vitamin E are dropped into oil phase pot and be heated to 75～80 ℃, stir until dissolved to homogeneous, 50 revs/min of mixing speeds;
(3) whole water is poured in water pot, then slowly pour glycerol, Oligomeric maltose base glucoside into, after stirring and being uniformly dispersed for 10 minutes, be sprinkled into hyaluronic acid, after stirring and being uniformly dispersed for 30 minutes, heating, is warmed up to 75～80 ℃ simultaneously, mixing speed is 50 revs/min, and raw material is incubated half an hour after all dissolving;
(4) first by the oil phase sucking filtration in oil phase pot to emulsifying pot, open to stir, mixing speed is 80 revs/min, at a slow speed when homogenizing again by the water sucking filtration in water pot to emulsifying pot, temperature keeps 75 ℃, after expecting the whole suction of body, fast homogeneous is 3 minutes; Homogenizing mixing speed is 2500 revs/min;
(5) stop homogenizing, cool to below 50 ℃, add successively essence, Aloe extraction solution, composite fermentation aminoacid entirely to imitate nutritional solution and stir, all add rear continuation to stir 30 minutes, in the time of cooling, omnidistance evacuation, stirs 50 revs/min;
(6) stir and be cooled to 38 ℃, stop stirring, check, discharging, obtain biofermentation late frost product.
The main component of biofermentation late frost of the present invention is that composite fermentation aminoacid is imitated nutritional solution entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligosaccharide, the nutrient substance of more than the 40 kind of needed by human such as the mineral such as soybean phospholipid, soybean saponin, VitC, VitE, B group vitamin, zinc, selenium, phosphorus, ferrum, calcium and trace element, beta-carotene, thiamine, citric acid.
Above-mentioned composite fermentation aminoacid is imitated nutritional solution entirely, raw material comprises following component and weight percent content: lactic acid bacterial liquid 0.5%, pink yeast liquid 0.5%, Phanerochaete chrysosporium bacterium liquid 0.5%, Bacillus licheniformis liquid 0.5%, Bacillus pumilus bacterium liquid 0.5%, maltose candidiasis liquid 0.5%, long shoot trichoderma liquid 0.5%, shatian pomelo 0.4%, the place of production is the Fructus Mali pumilae 0.4% in the Qinling Mountains, the place of production is the Fructus Musae 0.4% in Yunnan, Lac regis apis 0.2%, the place of production is the extraordinary Semen Glycines 1.0% in Shaanxi, Radix Ginseng 0.5%, Ganoderma 0.5%, lavandula angustifolia 1.0%, rice distiller grain 20%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactobacillus culture medium raw material, comprise following component and weight percent content: milk casein peptone 8%, peptone 8%, yeast extract 4%, glucose 4%, tween 80 0.8%, dipotassium hydrogen phosphate 1.8%, sodium acetate 4%, citric acid diamidogen 1.8%, magnesium sulfate 0.1%, manganese sulfate 0.04%, surplus are pure water;
(2) preparation of fluid medium: press lactobacillus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the lactobacillus inoculation of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the sterilized fluid medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink Yeast Cultivation based raw material, comprise following component and weight percent content: peptone 4.1%, glucose 8%, yeast extract 2%, surplus are beerwort;
(2) preparation of fluid medium: by pink Yeast Cultivation based formulas preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the pink barms of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9%, KH
2pO
40.25%, MgSO
47H
2o0.14%, vitaminB10 .01%, agar 1.4%, surplus are Rhizoma Solani tuber osi extracting solution;
(2) preparation of fluid medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Phanerochaete chrysosporium strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: the NaOH solution that wheat bran 4.5%, soybean cake powder 2.7%, agar 1.8%, surplus are 0.2wt%, after wheat bran, soybean cake powder are mixed, add 0.2wt%NaOH solution and boil 1h, with HCl, neutralizing pH is 7, then adds agar fusing;
(2) preparation of fluid medium: the sample that step (1) is obtained is at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus licheniformis strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare Bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4%, beef leaching thing 0.25%, NaCl0.4%, agar 1.4%, surplus are distilled water;
(2) preparation of fluid medium: by Bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus pumilus strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus pumilus bacterium liquid.
Maltose candidiasis liquid prepares by the following method:
(1) prepare maltose candida mycoderma culture medium raw material, comprise following component and weight percent content: agar 1.4%; 5 ° of beerworts 98.6%;
(2) preparation of fluid medium: press maltose candida mycoderma culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the maltose candida strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain maltose candidiasis liquid.
Long shoot trichoderma liquid prepares by the following method:
(1) prepare long shoot Trichoderma spp. culture medium raw material, comprise following component and weight percent content: agar 1.4%; 5 ° of beerworts 98.6%;
(2) preparation of fluid medium: by long shoot Trichoderma spp. culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the long shoot Trichoderma spp. strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain long shoot trichoderma liquid.
Composite fermentation aminoacid is imitated nutritional solution in the preparation entirely, according to formula, prepare after raw material, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtaining is taken out and filtered, collect filtrate and control temperature and be 110 ℃ and carry out high temperature sterilize, be finally cooled to room temperature, obtain composite fermentation aminoacid and entirely imitate nutritional solution.
Embodiment 2
A preparation method for biofermentation late frost, the method comprises the following steps:
(1) take by weight percentage: composite fermentation aminoacid is imitated nutritional solution 15% entirely; Oligomeric maltose base glucoside 3.5%; Natural Jojoba oil 6%; Glycerol 6%; Cetearyl alcohol 3.5%; Squalane 6%; Vitamin E 0.35%; Hyaluronic acid 0.06%; Aloe extraction solution 2.5%; Essence 0.3%; Water surplus;
(2) first cetearyl alcohol, natural Jojoba oil, squalane, vitamin E are dropped into oil phase pot and be heated to 75～80 ℃, stir until dissolved to homogeneous, 50 revs/min of mixing speeds;
(3) whole water is poured in water pot, then slowly pour glycerol, Oligomeric maltose base glucoside into, after stirring and being uniformly dispersed for 10 minutes, be sprinkled into hyaluronic acid, after stirring and being uniformly dispersed for 30 minutes, heating, is warmed up to 75～80 ℃ simultaneously, mixing speed is 50 revs/min, and raw material is incubated half an hour after all dissolving;
(4) first by the oil phase sucking filtration in oil phase pot to emulsifying pot, open to stir, mixing speed is 80 revs/min, at a slow speed when homogenizing again by the water sucking filtration in water pot to emulsifying pot, temperature keeps 75 ℃, after expecting the whole suction of body, fast homogeneous is 3 minutes; Homogenizing mixing speed is 2500 revs/min;
(5) stop homogenizing, cool to below 50 ℃, add successively essence, Aloe extraction solution, composite fermentation aminoacid entirely to imitate nutritional solution and stir, all add rear continuation to stir 30 minutes, in the time of cooling, omnidistance evacuation, stirs 50 revs/min;
(6) stir and be cooled to 38 ℃, stop stirring, check, discharging, obtain biofermentation late frost product.
The main component of biofermentation late frost of the present invention is that composite fermentation aminoacid is imitated nutritional solution entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligosaccharide, the nutrient substance of more than the 40 kind of needed by human such as the mineral such as soybean phospholipid, soybean saponin, VitC, VitE, B group vitamin, zinc, selenium, phosphorus, ferrum, calcium and trace element, beta-carotene, thiamine, citric acid.
Above-mentioned composite fermentation aminoacid is imitated nutritional solution entirely, comprise following component and weight percent content: lactic acid bacterial liquid 0.6%, pink yeast liquid 0.8%, Phanerochaete chrysosporium bacterium liquid 0.8%, Bacillus licheniformis liquid 0.6%, Bacillus pumilus bacterium liquid 0.7%, maltose candidiasis liquid 0.7%, long shoot trichoderma liquid 0.8%, shatian pomelo 0.5%, the Fructus Mali pumilae 0.5% in Dalian, the place of production, the Fructus Musae 0.5% in Yunnan, the place of production, Lac regis apis 0.2%, the extraordinary Semen Glycines 1.1% in Heilungkiang, the place of production, Radix Ginseng 0.6%, Ganoderma 0.6%, lavandula angustifolia 1.1%, rice distiller grain 22%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactobacillus culture medium raw material, comprise following component and weight percent content: milk casein peptone 10%, peptone 10%, yeast extract 5%, glucose 5%, tween 80 1%, dipotassium hydrogen phosphate 2%, sodium acetate 5%, citric acid diamidogen 2%, magnesium sulfate 0.2%, manganese sulfate 0.05%, surplus are pure water;
(2) preparation of fluid medium: press lactobacillus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the lactobacillus inoculation of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the sterilized fluid medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink Yeast Cultivation based raw material, comprise following component and weight percent content: peptone 5%, glucose 10%, yeast extract 3%, surplus are beerwort;
(2) preparation of fluid medium: by pink Yeast Cultivation based formulas preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the pink barms of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9%, KH
2pO
40.28%, MgSO
47H
2o0.14%, vitaminB10 .01%, agar 1.4%, surplus are Rhizoma Solani tuber osi extracting solution;
(2) preparation of fluid medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Phanerochaete chrysosporium strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: the NaOH solution that wheat bran 4.6%, soybean cake powder 2.8%, agar 1.8%, surplus are 0.2wt%, after wheat bran, soybean cake powder are mixed, add 0.2wt%NaOH solution and boil 1h, with HCl, neutralizing pH is 7, then adds agar fusing;
(2) preparation of fluid medium: the sample that step (1) is obtained is at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus licheniformis strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare Bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.5%, beef leaching thing 0.3%, NaCl0.5%, agar 1.4%, surplus are distilled water;
(2) preparation of fluid medium: by Bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus pumilus strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus pumilus bacterium liquid.
Maltose candidiasis liquid prepares by the following method:
(1) prepare maltose candida mycoderma culture medium raw material, comprise following component and weight percent content: agar 1.5%; 5 ° of beerworts 98.5%;
(2) preparation of fluid medium: press maltose candida mycoderma culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the maltose candida strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain maltose candidiasis liquid.
Long shoot trichoderma liquid prepares by the following method:
(1) prepare long shoot Trichoderma spp. culture medium raw material, comprise following component and weight percent content: agar 1.5%; 5 ° of beerworts 98.5%;
(2) preparation of fluid medium: by long shoot Trichoderma spp. culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the long shoot Trichoderma spp. strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain long shoot trichoderma liquid.
Composite fermentation aminoacid is imitated nutritional solution in the preparation entirely, according to formula, prepare after raw material, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtaining is taken out and filtered, collect filtrate and control temperature and be 110 ℃ and carry out high temperature sterilize, be finally cooled to room temperature, obtain composite fermentation aminoacid and entirely imitate nutritional solution.
Embodiment 3
A preparation method for biofermentation late frost, the method comprises the following steps:
(1) take by weight percentage: composite fermentation aminoacid is imitated nutritional solution 10% entirely; Oligomeric maltose base glucoside 3%; Natural Jojoba oil 5.5%; Glycerol 4%; Cetearyl alcohol 2.5%; Squalane 4%; Vitamin E 0.25%; Hyaluronic acid 0.04%; Aloe extraction solution 2.0%; Essence 0.2%; Water surplus;
(2) first cetearyl alcohol, natural Jojoba oil, squalane, vitamin E are dropped into oil phase pot and be heated to 75～80 ℃, stir until dissolved to homogeneous, 50 revs/min of mixing speeds;
(3) whole water is poured in water pot, then slowly pour glycerol, Oligomeric maltose base glucoside into, after stirring and being uniformly dispersed for 10 minutes, be sprinkled into hyaluronic acid, after stirring and being uniformly dispersed for 30 minutes, heating, is warmed up to 75～80 ℃ simultaneously, mixing speed is 50 revs/min, and raw material is incubated half an hour after all dissolving;
(4) first by the oil phase sucking filtration in oil phase pot to emulsifying pot, open to stir, mixing speed is 80 revs/min, at a slow speed when homogenizing again by the water sucking filtration in water pot to emulsifying pot, temperature keeps 75 ℃, after expecting the whole suction of body, fast homogeneous is 3 minutes; Homogenizing mixing speed is 2500 revs/min;
(5) stop homogenizing, cool to below 50 ℃, add successively essence, Aloe extraction solution, composite fermentation aminoacid entirely to imitate nutritional solution and stir, all add rear continuation to stir 30 minutes, in the time of cooling, omnidistance evacuation, stirs 50 revs/min;
(6) stir and be cooled to 38 ℃, stop stirring, check, discharging, obtain biofermentation late frost product.
The main component of biofermentation late frost of the present invention is that composite fermentation aminoacid is imitated nutritional solution entirely, it is rich in necessary 18 seed amino acids of skin cell, soybean lsoflavons, oligosaccharide, the nutrient substance of more than the 40 kind of needed by human such as the mineral such as soybean phospholipid, soybean saponin, VitC, VitE, B group vitamin, zinc, selenium, phosphorus, ferrum, calcium and trace element, beta-carotene, thiamine, citric acid.
Above-mentioned composite fermentation aminoacid is imitated nutritional solution entirely, comprise following component and weight percent content: lactic acid bacterial liquid 1.0%, pink yeast liquid 1.0%, Phanerochaete chrysosporium bacterium liquid 1.0%, Bacillus licheniformis liquid 1.0%, Bacillus pumilus bacterium liquid 1.0%, maltose candidiasis liquid 1.0%, long shoot trichoderma liquid 1.0%, shatian pomelo 0.6%, the Fructus Mali pumilae 0.6% in Dalian, the place of production, the Fructus Musae 0.6% in Yunnan, the place of production, Lac regis apis 0.3%, the extraordinary Semen Glycines 1.2% in Heilungkiang, the place of production, Radix Ginseng 0.9%, Ganoderma 0.9%, lavandula angustifolia 1.2%, rice distiller grain 25%, surplus is pure water.
Lactic acid bacterial liquid prepares by the following method:
(1) prepare lactobacillus culture medium raw material, comprise following component and weight percent content: milk casein peptone 12%, peptone 12%, yeast extract 6%, glucose 6%, tween 80 1.2%, dipotassium hydrogen phosphate 2.5%, sodium acetate 6%, citric acid diamidogen 2.5%, magnesium sulfate 0.3%, manganese sulfate 0.06%, surplus are pure water;
(2) preparation of fluid medium: press lactobacillus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the lactobacillus inoculation of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the sterilized fluid medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain lactic acid bacterial liquid.
Pink yeast liquid prepares by the following method:
(1) prepare pink Yeast Cultivation based raw material, comprise following component and weight percent content: peptone 6%, glucose 12%, yeast extract 4%, surplus are beerwort;
(2) preparation of fluid medium: by pink Yeast Cultivation based formulas preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the pink barms of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain pink yeast liquid.
Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 2.0%, KH
2pO
40.30%, MgSO
47H
2o0.15%, vitaminB10 .01%, agar 1.5%, surplus are Rhizoma Solani tuber osi extracting solution;
(2) preparation of fluid medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Phanerochaete chrysosporium strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Phanerochaete chrysosporium bacterium liquid.
Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: the NaOH solution that wheat bran 4.5～4.7%, soybean cake powder 2.7～2.85%, agar 1.8～1.85%, surplus are 0.2wt%, after wheat bran, soybean cake powder are mixed, add 0.2wt%NaOH solution and boil 1h, with HCl, neutralizing pH is 7, then adds agar fusing;
(2) preparation of fluid medium: the sample that step (1) is obtained is at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus licheniformis strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus licheniformis liquid.
Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare Bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.6%, beef leaching thing 0.35%, NaCl0.6%, agar 1.5%, surplus are distilled water;
(2) preparation of fluid medium: by Bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus pumilus strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus pumilus bacterium liquid.
Maltose candidiasis liquid prepares by the following method:
(1) prepare maltose candida mycoderma culture medium raw material, comprise following component and weight percent content: agar 1.6%; 5 ° of beerworts 98.4%;
(2) preparation of fluid medium: press maltose candida mycoderma culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the maltose candida strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain maltose candidiasis liquid.
Long shoot trichoderma liquid prepares by the following method:
(1) prepare long shoot Trichoderma spp. culture medium raw material, comprise following component and weight percent content: agar 1.6%; 5 ° of beerworts 98.4%;
(2) preparation of fluid medium: by long shoot Trichoderma spp. culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the long shoot Trichoderma spp. strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain long shoot trichoderma liquid.
Composite fermentation aminoacid is imitated nutritional solution in the preparation entirely, according to formula, prepare after raw material, raw material pulverizing is puddled evenly, be placed in pottery static fermentation 30 days, then the product obtaining is taken out and filtered, collect filtrate and control temperature and be 110 ℃ and carry out high temperature sterilize, be finally cooled to room temperature, obtain composite fermentation aminoacid and entirely imitate nutritional solution.
Following table by the nutritional labeling of acquisition biofermentation late frost.
| Nutrition title | Content (mg/ml) | Nutrition title | Content (mg/ml) |
| Aspartic acid | 0.152 | First sulfur | 0.017 |
| Threonine | 0.035 | V-ABA | 0.097 |
| Serine | 0.023 | Glucose | 0.11％ |
| Glutamic acid | 0.099 | Maltose | 0.11％ |
| Proline | 0.083 | Citric acid | 0.13％ |
| Glycine | 0.084 | VA | 0.13 |
| Alanine | 0.118 | VB2 | 0.06 |
| Cystine | 0.073 | VB6 | 0.09 |
| Valine | 0.111 | VB12 | 0.075 |
| Isoleucine | 0.081 | VC | 0.58 |
| Leucine | 0.126 | VE | 0.12 |
| Tyrosine | 0.015 | Crude fibre | 0.015 |
| Phenylalanine | 0.096 | Zinc | 0.075mg/100g |
| Ornithine | 0.021 | Selenium | 0.89ug/100g |
| Lysine | 0.036 | Ferrum | 0.045mg/100g |
| Tryptophan | 0.012 | Calcium | 0.085mg/100g |
| Arginine | 0.035 | Soybean isoflavone | 0.23ug/ml |
| Histidine | 0.032 | Soybean saponin | 0.12ug/ml |
| Thiamine | 0.47ug/ml | Soybean phospholipid | 0.24ug/ml |
| Ginsenoside | 0.13ug/ml | Beta-carotene | 0.32 |
| Ergosterol | 0.08ug/ml | ? | ? |
。
Claims (10)
1. a biofermentation late frost, is characterized in that, comprises the component of following weight percentage:
Composite fermentation aminoacid is imitated nutritional solution 10%～15% entirely;
Oligomeric maltose base glucoside 3%～3.5%;
Natural Jojoba oil 5.5%～6%;
Glycerol 4%～6%;
Cetearyl alcohol 2.5%～3.5%;
Squalane 4%～6%;
Vitamin E 0.25%～0.35%;
Hyaluronic acid 0.04%～0.06%;
Aloe extraction solution 2.0%～2.5%;
Essence 0.2%～0.3%;
Water surplus.
2. a kind of biofermentation late frost according to claim 1, it is characterized in that, described composite fermentation aminoacid is entirely imitated nutritional solution and is made by the following method: raw material is placed in to pottery static fermentation 21～30 days, then the product obtaining is taken out and filtered, collect filtrate and control temperature and be 100～110 ℃ and carry out high temperature sterilize, finally be cooled to room temperature, obtain composite fermentation aminoacid and entirely imitate nutritional solution.
3. a kind of biofermentation late frost according to claim 2, it is characterized in that, described raw material comprises the component of following weight percent content: lactic acid bacterial liquid 0.5～1.0%, pink yeast liquid 0.5～1.0%, Phanerochaete chrysosporium bacterium liquid 0.5～1.0%, Bacillus licheniformis liquid 0.5～1.0%, Bacillus pumilus bacterium liquid 0.5～1.0%, maltose candidiasis liquid 0.5～1.0%, long shoot trichoderma liquid 0.5～1.0%, shatian pomelo 0.4～0.6%, Fructus Mali pumilae 0.4～0.6%, Fructus Musae 0.4～0.6%, Lac regis apis 0.2～0.3%, extraordinary Semen Glycines 1.0～1.2%, Radix Ginseng 0.5～0.9%, Ganoderma 0.5～0.9%, lavandula angustifolia 1.0～1.2%, rice distiller grain 20～25%, surplus is pure water.
4. a kind of biofermentation late frost according to claim 3, is characterized in that, described lactic acid bacterial liquid prepares by the following method:
(1) prepare lactobacillus culture medium raw material, comprise following component and weight percent content: milk casein peptone 8～12%, peptone 8～12%, yeast extract 4～6%, glucose 4～6%, tween 80 0.8～1.2%, dipotassium hydrogen phosphate 1.8～2.5%, sodium acetate 4～6%, citric acid diamidogen 1.8～2.5%, magnesium sulfate 0.1～0.3%, manganese sulfate 0.04～0.06%, surplus are pure water;
(2) preparation of fluid medium: press lactobacillus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the lactobacillus inoculation of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the sterilized fluid medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain lactic acid bacterial liquid.
5. a kind of biofermentation late frost according to claim 3, is characterized in that, described pink yeast liquid prepares by the following method:
(1) prepare pink Yeast Cultivation based raw material, comprise following component and weight percent content: peptone 4.1～6%, glucose 8～12%, yeast extract 2～4%, surplus are beerwort;
(2) preparation of fluid medium: by pink Yeast Cultivation based formulas preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the pink barms of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain pink yeast liquid.
6. a kind of biofermentation late frost according to claim 3, is characterized in that, described Phanerochaete chrysosporium bacterium liquid prepares by the following method:
(1) prepare Phanerochaete chrysosporium culture medium raw material, comprise following component and weight percent content: glucose 1.9～2.0%, KH
2p0
40.25～0.30%, MgSO
47H
2o0.14～0.15%, vitaminB10 .01%, agar 1.4～1.5%, surplus are Rhizoma Solani tuber osi extracting solution;
(2) preparation of fluid medium: by Phanerochaete chrysosporium culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Phanerochaete chrysosporium strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Phanerochaete chrysosporium bacterium liquid.
7. a kind of biofermentation late frost according to claim 3, is characterized in that, described Bacillus licheniformis liquid prepares by the following method:
(1) Bacillus licheniformis culture medium raw material comprises following component and weight percent content: the NaOH solution that wheat bran 4.5～4.7%, soybean cake powder 2.7～2.85%, agar 1.8～1.85%, surplus are 0.2wt%, after wheat bran, soybean cake powder are mixed, add 0.2wt%NaOH solution and boil 1h, with HCl, neutralizing pH is 7, then adds agar fusing;
(2) preparation of fluid medium: the sample that step (1) is obtained is at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus licheniformis strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus licheniformis liquid.
8. a kind of biofermentation late frost according to claim 3, is characterized in that, described Bacillus pumilus bacterium liquid prepares by the following method:
(1) prepare Bacillus pumilus culture medium raw material, comprise following component and weight percent content: peptone 0.4～0.6%, beef leaching thing 0.25～0.35%, NaCl0.4～0.6%, agar 1.4～1.5%, surplus are distilled water;
(2) preparation of fluid medium: by Bacillus pumilus culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the Bacillus pumilus strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain Bacillus pumilus bacterium liquid.
9. a kind of biofermentation late frost according to claim 3, is characterized in that, described maltose candidiasis liquid prepares by the following method:
(1) prepare maltose candida mycoderma culture medium raw material, comprise following component and weight percent content: agar 1.4～1.6%; 5 ° of beerworts 98.4～98.6%;
(2) preparation of fluid medium: press maltose candida mycoderma culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the maltose candida strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain maltose candidiasis liquid;
Described long shoot trichoderma liquid prepares by the following method:
(1) prepare long shoot Trichoderma spp. culture medium raw material, comprise following component and weight percent content: agar 1.4～1.6%; 5 ° of beerworts 98.4～98.6%;
(2) preparation of fluid medium: by long shoot Trichoderma spp. culture medium prescription preparation raw material, at 0.15MPa, 121 ℃, sterilizing 30min, obtains the fluid medium of sterilizing, is placed in fermentation tank;
(3) preparation of shaking flask strain: get the long shoot Trichoderma spp. strain of preserving on inclined-plane and access in gnotobasis in sterilized fluid medium, at 30 ℃, 100～200 times/min of the rate of shaking, carries out shake-flask culture 48h, obtains shaking flask bacterium liquid;
(4) produce the preparation of bacterium liquid: press 3wt%～4wt% inoculum concentration by shaking flask bacterium liquid access fermentation tank, at 30 ℃, ventilating ratio is to ferment under 1: 1 (V/V) liquid amount condition that is 1/3, after 48h, by above-mentioned condition, add again the culture medium continuation fermentation of 1/3 volume, after 96h, stop fermentation, obtain long shoot trichoderma liquid;
Described Fructus Mali pumilae is the Fructus Mali pumilae of selecting the Qinling Mountains or Dalian,
Described Fructus Musae is selected the Fructus Musae in Yunnan,
Described extraordinary Semen Glycines is selected the extraordinary Semen Glycines into Shaanxi or Heilungkiang.
10. a preparation method for biofermentation late frost as claimed in claim 1, is characterized in that, the method comprises the following steps:
(1) take by weight percentage: composite fermentation aminoacid is imitated nutritional solution 10%～15% entirely; Oligomeric maltose base glucoside 3%～3.5%; Natural Jojoba oil 5.5%～6%; Glycerol 4%～6%; Cetearyl alcohol 2.5%～3.5%; Squalane 4%～6%; Vitamin E 0.25%～0.35%; Hyaluronic acid 0.04～0.06%; Aloe extraction solution 2.0%～2.5%; Essence 0.2%～0.3%; Water surplus;
(2) first cetearyl alcohol, natural Jojoba oil, squalane, vitamin E are dropped into oil phase pot and be heated to 75～80 ℃, stir until dissolved to homogeneous, 50 revs/min of mixing speeds;
(3) whole water is poured in water pot, then slowly pour glycerol, Oligomeric maltose base glucoside into, after stirring and being uniformly dispersed for 10 minutes, be sprinkled into hyaluronic acid, after stirring and being uniformly dispersed for 30 minutes, heating, is warmed up to 75～80 ℃ simultaneously, mixing speed is 50 revs/min, and raw material is incubated half an hour after all dissolving;
(4) first by the oil phase sucking filtration in oil phase pot to emulsifying pot, open to stir, mixing speed is 80 revs/min, at a slow speed when homogenizing again by the water sucking filtration in water pot to emulsifying pot, temperature keeps 75 ℃, after expecting the whole suction of body, fast homogeneous is 3 minutes; Homogenizing mixing speed is 2500 revs/min;
(5) stop homogenizing, cool to below 50 ℃, add successively essence, Aloe extraction solution, composite fermentation aminoacid entirely to imitate nutritional solution and stir, all add rear continuation to stir 30 minutes, in the time of cooling, omnidistance evacuation, stirs 50 revs/min;
(6) stir and be cooled to 38 ℃, stop stirring, check, discharging, obtain biofermentation late frost product.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060066151A (en) * | 2006-05-16 | 2006-06-15 | (주)로힐 | Method of producing a nutrition cream with anti-wrinkle effect using sea cucumber extract |
| CN101991523A (en) * | 2010-05-19 | 2011-03-30 | 黄汉贤 | Specific anti-wrinkle whitening cream and preparation method thereof |
-
2013
- 2013-05-24 CN CN201310199696.0A patent/CN103655438B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060066151A (en) * | 2006-05-16 | 2006-06-15 | (주)로힐 | Method of producing a nutrition cream with anti-wrinkle effect using sea cucumber extract |
| CN101991523A (en) * | 2010-05-19 | 2011-03-30 | 黄汉贤 | Specific anti-wrinkle whitening cream and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 梅乐和等: "《生化生产工艺学》", 31 August 1999, article "培养基的分类", pages: 32-33 * |
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