CN103651142B - Stem tip stripping technology of sweet potato - Google Patents
Stem tip stripping technology of sweet potato Download PDFInfo
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- CN103651142B CN103651142B CN201310678323.1A CN201310678323A CN103651142B CN 103651142 B CN103651142 B CN 103651142B CN 201310678323 A CN201310678323 A CN 201310678323A CN 103651142 B CN103651142 B CN 103651142B
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- sweet potato
- stem tip
- leaf
- stem
- primordiums
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Abstract
The invention relates to a stem tip stripping technology of a sweet potato, belonging to the field of biotechnology. The stem tip stripping technology of the sweet potato specifically comprises the following steps: cultivating a sweet potato block in a greenhouse or a manual climatic box; selecting a sweet potato branch which is vigorous in growth, and disinfecting for later use; stripping the stem tip of a terminal bud of the sweet potato branch layer by layer on an ultra-clean working table till 5-6 leaf primordiums of the branch are left; cutting a place 0.6-0.7mm from the stem tip by a scalpel, wherein the third to fifth leaf primordiums in the outer layer are naturally separated from the stem tip with the first to second leaf primordiums; and removing the leaf primordiums on both sides of the stem tip or directly picking out the stem tip by a dissecting needle to obtain the stripped sweet potato stem tip with 1-2 leaf primordiums as required. According to the stem tip stripping technology of the sweet potato provided by the invention, when 5-6 leaf primordiums are left, the stem tip is cut, so that the stripping speed is high. At least 5-6 leaf primordiums protect the outer side of the stem tip in a stripping process, so that the stem tip is unlikely to dry and high in survival rate.
Description
Technical field
The present invention relates to sweet potato stem tip lift-off technology, belong to biological technical field.
Background technology
China is sweet potato producing country the biggest in the world, cultivated area and gross yield account for 60% and 85% of the world respectively, and the long-term cultivated area of sweet potato, more than 5,500,000 hectares, is only second to paddy rice, wheat and maize, gross yield reaches 1.5 hundred million tons, for national food security plays positive role.During current China sweet potato produces, the recommended variety of about 40% is only had to carry out detoxification.
Sweet potato is asexually propagated crop, must pass through Shoot Tip Culture to carry out detoxification, the size that stem apex is peeled off determines the removal efficiency of virus, but stem apex stripping is less, and splitting time is longer, stem apex is easily dry, the survival rate of cultivating is lower, and traditional stripping means adopts dissecting needle and tweezers to peel off layer by layer sweet potato stem tip, until surplus 1-2 sheet leaf primordium, easily cause stem apex to destroy, and peel off difficulty.
Summary of the invention
The object of the invention is to the deficiency solving the existence of above-mentioned prior art, the sweet potato stem tip lift-off technology that a kind of peeling rate is fast, survival rate is high is provided.
Sweet potato stem tip lift-off technology of the present invention, special character is to comprise following concrete steps:
(1), sweet potato block cultivated by greenhouse or climatic cabinate;
(2), the sweet potato branch of vigorous growth is selected, for subsequent use after sterilization;
(3), under superclean bench sweet potato branch terminal bud stem apex is peeled off layer by layer, to branch residue 5-6 sheet leaf primordium;
(4), adopt scalpel under distance stem apex 0.6-0.7mm place imposes uniformity without examining individual cases, outer 3-5 sheet leaf primordium and the sweet potato stem tip natural separation being with 1-2 sheet leaf primordium;
(5), adopt dissecting needle remove the leaf primordium of stem apex both sides or directly choose stem apex, obtain the sweet potato stem tip of the required band 1-2 sheet leaf primordium peeled off.
Sweet potato stem tip lift-off technology of the present invention, under imposing uniformity without examining individual cases when remaining 5-6 sheet leaf primordium, peeling rate is fast, and in stripping process, have the protection of 5-6 sheet leaf primordium outside stem apex at least, and stem apex is not easily dry, and survival rate is high.
Embodiment
Below provide the specific embodiment of the present invention, be used for being further described the present invention.
Embodiment 1
The sweet potato stem tip lift-off technology of the present embodiment comprises following concrete steps:
(1), hot-house culture sweet potato block;
(2), the sweet potato branch of vigorous growth is selected, for subsequent use after sterilization on request;
(3), adopt dissecting needle and tweezers to peel off layer by layer sweet potato branch terminal bud stem apex under superclean bench, remain 6 leaf primordium to branch;
(4), adopt scalpel under distance stem apex 0.7mm place imposes uniformity without examining individual cases, outer 4th leaf primordium and stem apex (being with 2 leaf primordium) natural separation;
(five, there is enough clearance spaces in the leaf primordium of dispersion, adopt dissecting needle directly to shift out the stem apex of leaf primordium central authorities from side, the required stem apex (being with 2 leaf primordium) peeled off can be obtained.
Embodiment 2
The sweet potato stem tip lift-off technology of the present embodiment comprises following concrete steps:
(1), sweet potato block cultivated by climatic cabinate;
(2), the sweet potato branch of vigorous growth is selected, for subsequent use after sterilization on request;
(3), adopt dissecting needle and tweezers under superclean bench, sweet potato branch terminal bud stem apex is peeled off layer by layer, remains 5 leaf primordium to branch;
(4), adopt scalpel under stem apex 0.6mm place imposes uniformity without examining individual cases, outer 3rd leaf primordium and stem apex (being with 2 leaf primordium) natural separation;
(5), there is enough clearance spaces in the leaf primordium of dispersion, adopts dissecting needle directly to choose the stem apex of leaf primordium central authorities, can obtain the required stem apex (being with 2 leaf primordium) peeled off.
Embodiment 3
The sweet potato stem tip lift-off technology of the present embodiment comprises following concrete steps:
(1), hot-house culture sweet potato block;
(2), the sweet potato branch of vigorous growth is selected, for subsequent use after sterilization on request;
(3), adopt dissecting needle and tweezers under superclean bench, sweet potato branch terminal bud stem apex is peeled off layer by layer, remains 6 leaf primordium to branch;
(4), adopt scalpel under distance stem apex 0.7mm place imposes uniformity without examining individual cases, outer 5th leaf primordium and stem apex (being with 1 leaf primordium) natural separation;
(5), there is enough clearance spaces in the leaf primordium of dispersion, adopts dissecting needle directly to shift out the stem apex of leaf primordium central authorities from side, can obtain the required stem apex (being with 1 leaf primordium) peeled off.
Because superclean bench constantly outwards blows sterile wind, as leaf primordium is very few, the operating time is long, all easily make stem apex dry, be unfavorable for surviving of stem apex, therefore the present invention remains 5-6 sheet leaf primordium, keep the moistening of sweet potato stem tip, improve the survival rate of sweet potato stem tip.
Sweet potato stem tip lift-off technology of the present invention, under imposing uniformity without examining individual cases when remaining 5-6 sheet leaf primordium, peeling rate is fast, and in stripping process, have the protection of 5-6 sheet leaf primordium outside stem apex at least, and stem apex is not easily dry, and survival rate is high.
Claims (1)
1. sweet potato stem tip stripping means, is characterized in that comprising following concrete steps:
(1), sweet potato block cultivated by greenhouse or climatic cabinate;
(2), the sweet potato branch of vigorous growth is selected, for subsequent use after sterilization;
(3), at superclean bench sweet potato branch terminal bud stem apex is peeled off layer by layer, to branch residue 5-6 sheet leaf primordium;
(4), adopt scalpel under distance stem apex 0.6-0.7mm place imposes uniformity without examining individual cases, outer 3-5 sheet leaf primordium and the sweet potato stem tip natural separation being with 1-2 sheet leaf primordium;
(5), adopt dissecting needle remove the leaf primordium of stem apex both sides or directly choose stem apex, obtain the sweet potato stem tip of the required band 1-2 sheet leaf primordium peeled off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310678323.1A CN103651142B (en) | 2013-12-14 | 2013-12-14 | Stem tip stripping technology of sweet potato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310678323.1A CN103651142B (en) | 2013-12-14 | 2013-12-14 | Stem tip stripping technology of sweet potato |
Publications (2)
| Publication Number | Publication Date |
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| CN103651142A CN103651142A (en) | 2014-03-26 |
| CN103651142B true CN103651142B (en) | 2015-07-15 |
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| CN201310678323.1A Active CN103651142B (en) | 2013-12-14 | 2013-12-14 | Stem tip stripping technology of sweet potato |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115136769B (en) * | 2022-07-26 | 2024-03-12 | 石家庄市农林科学研究院 | Sweet potato latent virus removal method |
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2013
- 2013-12-14 CN CN201310678323.1A patent/CN103651142B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| 何凤发,等.甘薯茎尖脱毒与快速繁殖技术研究.《西南农业大学学报》.2002,第24卷(第6期),全文. * |
| 我国马铃薯茎尖培养脱毒和脱毒试管苗微繁研究进展;郝文胜等;《内蒙古农业科技》;20111231;第27页左栏第1.1.1节，第28页右栏第1.2节第1段，第28页右栏最后一行-第29页左栏第1段 * |
| 甘薯茎尖脱毒技术研究进展;蒋明权,等;《安徽农业科学》;20030228;第31卷(第6期);全文 * |
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| CN103651142A (en) | 2014-03-26 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Sweet potato shoot tip peeling method Effective date of registration: 20211123 Granted publication date: 20150715 Pledgee: Yantai Fushan huiran Private Capital Management Co.,Ltd. Pledgor: SHANDONG YANTAI AGRICULTURAL SCI & TECH INSTITUTE Registration number: Y2021980012999 |
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Date of cancellation: 20220505 Granted publication date: 20150715 Pledgee: Yantai Fushan huiran Private Capital Management Co.,Ltd. Pledgor: SHANDONG YANTAI AGRICULTURAL SCI & TECH INSTITUTE Registration number: Y2021980012999 |
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