CN104106470A - Preparation method of porphyra yezoensis pure line germplasm protonema - Google Patents
Preparation method of porphyra yezoensis pure line germplasm protonema Download PDFInfo
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- CN104106470A CN104106470A CN201410318217.7A CN201410318217A CN104106470A CN 104106470 A CN104106470 A CN 104106470A CN 201410318217 A CN201410318217 A CN 201410318217A CN 104106470 A CN104106470 A CN 104106470A
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- thallus
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- porphyra yezoensis
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Abstract
The invention relates to a method for rapidly preparing porphyra yezoensis pure line germplasm protonema. The method comprises the following steps: (1) selecting thallus formed by germination of porphyra yezoensis targeted character monospores; (2) separating and cutting the tissue of the thallus for culture to obtain the pure line germplasm protonema. According to the method, the germplasm breeding time is shortened, the directional breeding efficiency is high, the disadvantage of difficulty of obtaining the pure line in the traditional porphyra breeding process can be compensated and the conditions and the possibility of obtaining the porphyra yezoensis pure line seeds with good traits can be created.
Description
Technical field
The invention belongs to laver breeding field, particularly a kind of preparation method of porphyra yezoensis pure lines germplasm filamentous.
Background technology
Thallus of porphyra is the haploid generation in its history of life, and its seedling stage is the amphiploid generation.Porphyra yezoensis in its history of life, except having sexual reproduction, also has asexual reproduction as cultivated species.The histocyte at its thallus front end of spot laver thallus seedling stage can transform the vegetative spore-single spore of formation, thisly by 1 cell, form 1 single spore, and the spore that diffuses out can be sprouted formation thallus, single spore is different from the chimeric individuality of four molecules that conchospore germination generates, the individuality of being sprouted by single spore is homotype haplobiont, has identical genetic background.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method of preparing fast porphyra yezoensis pure lines germplasm filamentous, and the pure lines germplasm filamentous that the method prepares can be preserved all the year round as provenance, the use of can increasing at any time; The time that has not only shortened germplasm seed selection, directive breeding efficiency is high, has made up especially the deficiency that difficulty in traditional laver Breeding Process obtains pure lines, and the porphyra yezoensis pure lines germplasm that makes to obtain more tool merits has been created condition and may.
A kind of method of preparing fast porphyra yezoensis pure lines germplasm filamentous of the present invention, comprising:
(1) at porphyra yezoensis single spore, sprout in the thallus forming and choose objective trait thallus;
(2) thallus through cleaning, dry in the shade, freezing, thallus is taken out after freezing, then after cleaning, separation cuts tissue;
(3) separation cuts tissue and cultivates, and must be sheerly germplasm filamentous.
In described step (1), objective trait thallus must be to sprout by single spore the thallus forming.
It must be thallus vegetative tissue that the middle separation of described step (2) cuts tissue.
The condition of culture that separation in described step (3) cuts tissue is: the low light level 1,000～2,000Lx, 12L:12D, 15～18 ℃ of temperature, culture fluid PES culture fluid or boil seawater and add static cultivation under the condition of N, P.
The thallus tissue that the present invention chooses, cell is growth or division slowly, and some forms megabacterium, and some splits into cell mass or cell lump, cultivation results shows, these occur there are no gamogenesis cell by thallus trophozyte, but through the development pathway of similar callus, by anogamic propagation, filamentous have directly occurred, these filamentouss can be as normal filamentous, manage to support and grow to sporangial branchlets, the ripe rear conchospore that forms, diffuses out afterwards and sprouts and grow up to little laver.By this method, can obtain then pure lines germplasm filamentous, simplify and shortened laver germplasm preparation procedure.
beneficial effect
The pure lines germplasm filamentous that the present invention prepares can be preserved all the year round as provenance, the use of can increasing at any time; The present invention has not only shortened the time of germplasm seed selection, and directive breeding efficiency is high, has made up especially the deficiency that difficulty in traditional laver Breeding Process obtains pure lines, and the porphyra yezoensis pure lines germplasm that makes to obtain more tool merits has been created condition and may.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
(1) at porphyra yezoensis single spore, sprout in the thallus forming and choose objective trait thallus;
(2) thallus is taken out thallus through cleaning, dry in the shade, after freezing, then after cleaning, separation cuts thallophytic vegetative tissue;
(3) by tissue at the low light level 1,000～2,000Lx, 12L: 12D, 15～18 ℃, PES culture fluid (Provasoli, 1963), or boil seawater and add under the culture fluid condition of N, P and cultivate, must be sheerly germplasm filamentous.
The preparation of most of laver germplasm such as laver Germplasm Bank as national in porphyra yezoensis strain " Y-L0601 ", " Y-L06DO " etc.
Claims (4)
1. a method of preparing fast porphyra yezoensis pure lines germplasm filamentous, comprising:
(1) at porphyra yezoensis single spore, sprout in the thallus forming and choose objective trait thallus;
(2) thallus through cleaning, dry in the shade, freezing, thallus is taken out after freezing, then after cleaning, separation cuts tissue;
(3) separation cuts tissue and cultivates, and must be sheerly germplasm filamentous.
2. the preparation method of a kind of porphyra yezoensis pure lines germplasm filamentous according to claim 1, is characterized in that: described step (1) objective trait thallus is to sprout by single spore the thallus forming.
3. the preparation method of a kind of porphyra yezoensis pure lines germplasm filamentous according to claim 1, is characterized in that: it is thallus vegetative tissue that described step (2) separation cuts tissue.
4. a kind of porphyra yezoensis according to claim 1 is sheerly the preparation method of germplasm filamentous, it is characterized in that: the condition of culture in described step (3) is: at the low light level 1,000～2,000Lx, 12L:12D, 15～18 ℃ of temperature, culture fluid PES culture fluid or boil seawater and add static cultivation under the condition of N, P.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410318217.7A CN104106470A (en) | 2014-07-04 | 2014-07-04 | Preparation method of porphyra yezoensis pure line germplasm protonema |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410318217.7A CN104106470A (en) | 2014-07-04 | 2014-07-04 | Preparation method of porphyra yezoensis pure line germplasm protonema |
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| CN104106470A true CN104106470A (en) | 2014-10-22 |
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| CN201410318217.7A Pending CN104106470A (en) | 2014-07-04 | 2014-07-04 | Preparation method of porphyra yezoensis pure line germplasm protonema |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719120A (en) * | 2015-02-13 | 2015-06-24 | 连云港市海洋与水产科学研究所 | Outdoor roller type porphyra yezoensis conchospore seedling collecting method |
| CN105993909A (en) * | 2016-05-13 | 2016-10-12 | 集美大学 | Pyropia haitanensis pure line seedling cultivation method |
| CN106148196A (en) * | 2016-06-29 | 2016-11-23 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | A kind of cultural method being sheerly Porphyra yezoensis Ueda |
| CN110839525A (en) * | 2019-10-31 | 2020-02-28 | 江苏省海洋水产研究所 | Preparation method of rhodobryum roseum germplasm filament |
| CN116491408A (en) * | 2023-06-25 | 2023-07-28 | 中国海洋大学三亚海洋研究院 | Method for efficiently preparing purple menu spores and application thereof |
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| CN1582642A (en) * | 2004-06-15 | 2005-02-23 | 中国海洋大学 | Breeding method for pure filement of laver |
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| JP2008125422A (en) * | 2006-11-20 | 2008-06-05 | Mie Prefecture | Method for converting dried laver seaweed into single cell form, and culturing the same |
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Patent Citations (4)
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| CN1582642A (en) * | 2004-06-15 | 2005-02-23 | 中国海洋大学 | Breeding method for pure filement of laver |
| CN1843093A (en) * | 2006-04-29 | 2006-10-11 | 宁波大学 | Laver quick breeding method |
| JP2008125422A (en) * | 2006-11-20 | 2008-06-05 | Mie Prefecture | Method for converting dried laver seaweed into single cell form, and culturing the same |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719120A (en) * | 2015-02-13 | 2015-06-24 | 连云港市海洋与水产科学研究所 | Outdoor roller type porphyra yezoensis conchospore seedling collecting method |
| CN105993909A (en) * | 2016-05-13 | 2016-10-12 | 集美大学 | Pyropia haitanensis pure line seedling cultivation method |
| CN105993909B (en) * | 2016-05-13 | 2019-08-27 | 集美大学 | A kind of porphyra haitanensis pure lines seedling fostering method |
| CN106148196A (en) * | 2016-06-29 | 2016-11-23 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | A kind of cultural method being sheerly Porphyra yezoensis Ueda |
| CN110839525A (en) * | 2019-10-31 | 2020-02-28 | 江苏省海洋水产研究所 | Preparation method of rhodobryum roseum germplasm filament |
| CN116491408A (en) * | 2023-06-25 | 2023-07-28 | 中国海洋大学三亚海洋研究院 | Method for efficiently preparing purple menu spores and application thereof |
| CN116491408B (en) * | 2023-06-25 | 2023-09-05 | 中国海洋大学三亚海洋研究院 | Method for efficiently preparing purple menu spores and application thereof |
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Application publication date: 20141022 |