CN103645130A - High flux quantitative analysis method for visible components in human or animal excretion - Google Patents
High flux quantitative analysis method for visible components in human or animal excretion Download PDFInfo
- Publication number
- CN103645130A CN103645130A CN201310532007.3A CN201310532007A CN103645130A CN 103645130 A CN103645130 A CN 103645130A CN 201310532007 A CN201310532007 A CN 201310532007A CN 103645130 A CN103645130 A CN 103645130A
- Authority
- CN
- China
- Prior art keywords
- human
- animal
- sample
- suspension
- analysis method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a high flux quantitative analysis method for visible components in human or animal excretion, which comprises the following steps: sampling with limitation, quantitative dilution, stirring, measurement, injection into counting cell, image acquisition and analysis, etc. The invention has the advantages that the method can be used for quantitative analysis and can precisely count number of visible components in per volume or weight of human or animal excretion with accurate result, thereby avoiding uncertainty in traditional sampling analysis method.
Description
Technical field
The present invention relates to medical inspection field, relate in particular to a kind of visible component High-throughput quantitative analysis method in human or animal's excretion thing.
Background technology
At present, collection and check analysis process for human or animal's excretion thing are as follows: get a part of excretion thing and be put in container, in carton or plastic cup, collection personnel send container into control laboratory, reviewer drops in 2 dilutions on slide, with the sample that cotton swab or little bamboo chip are got mung bean size from container, be coated on slide again, stir, then put and examine under a microscope.Observe the proterties of science such as color, hardness, mucus of sample simultaneously, with low power lens, observe the quantity of worm's ovum, as the worm's ovum quantity of protozoon, hook worm, roundworm, then observe the visible component in sample with high power lens, as the quantity of red blood cell, leucocyte, mould mycelia or special crystallization.The inspection situation of occulting blood as need,, with getting sample drop after above dilution on test paper, observes the situation of change of test paper.Then according to the result of above-mentioned observation, provide survey report, the content of survey report comprises the proterties of sample, as color, hardness, mucus, also comprises the microscopy result of sample, as contains how many worm's ovums, how many red blood cells etc., and the test findings of occulting blood.Be there is bio-safety hidden danger, operate the shortcomings such as unhygienic, smell is large in the collection of human or animal's excretion thing and check analysis process above, testing result is unstable, be difficult to the same assay of repetition, same sample is carried out to the data of multi collect check, deviation is very large, and operating process can not standardization.
Summary of the invention
The object of the invention is to overcome existing methodological deficiency, a kind of bio-safety standard, operating process standardization, testing result interior visible component High-throughput quantitative analysis method of human or animal's excretion thing accurately that meets is provided.
The present invention is achieved by the following technical programs: visible component High-throughput quantitative analysis method in human or animal's excretion thing, is characterized in that: it comprises the following steps:
(1) by sampler, get human or animal's excretion thing of limiting the quantity of, as sample to be checked;
(2) get the container of a known volume or weight, quantitative dilution and sample to be checked are joined in this container;
(3) dilution and sample to be checked are stirred, form suspension;
(4) with surveying instrument to adding volume of a container or the weight of dilution and sample to be checked accurately to measure;
(5) get a check-out console, check-out console middle part is provided with the counting chamber of a known depth, and suspension is filled with to counting chamber;
(6) counting chamber of filling with suspension is placed under the microscope of known visual field area and carries out image acquisition analysis.
Further, sampler in described step (1) is that vaned sampling rod is established in a lower end, described blade has a plurality of, in centre, be bound up, be air vane sheet, mutually between interval form the area of space of fixed size, sampling rod is inserted in human or animal's excretion thing, rotation sampling rod, excretion thing is adopted the region between blade.
Further, stirring in described step (3) is to adopt the mode of magnetic agitation to carry out, first magnetic bodies is added in the container that installs dilution and sample to be checked, again in the additional magnetic field constantly changing of container, magnetic bodies is constantly rotated under variable magnetic force effect, dilution and sample to be checked are fully mixed, form suspension.
Preferably, the surveying instrument described in described step (4) is CCD scanning sensor, with CCD scanning sensor, the suspension volume in container is accurately measured.
Preferably, the surveying instrument described in described step (4) is analytical balance, with analytical balance, the container that comprises suspension is carried out to accurate weighing.
Further, check-out console in described step (5) is continuous flow check-out console, described continuous flow check-out console comprises inlet tube, counting chamber and outlet, described inlet tube and outlet are located at counting chamber two ends, and be communicated with counting chamber, the degree of depth of described counting chamber is known numeric value, and suspension enters counting chamber by inlet tube, carries out under the microscope image acquisition.
Preferably, above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure.
Good effect of the present invention is:
(1) compare with existing analytical approach, the advantage of maximum of the present invention is can quantitative test, can definitely measure in human or animal's excretion thing unit volume or the visible component quantity of unit weight, its result degree of accuracy is high, error is little, representativeness is strong, can avoid the uncertainty of traditional sampling and analyzing method, the one-sidedness of analysis result, prevents mistaken diagnosis.
(2) the inventive method process is rigorous, has quantitatively, can realize scientific, the standardization of sample analysis process.
(3) the present invention adopts sampling rod sampling, and the bottom of sampling rod is the sampling head of air vane chip, and operating personnel only need rotate sampling rod, and sample is adopted the region between middle, and the quantity of sampling is comparatively stable, operating process simply, health, science.
(4) the present invention adopts magnetic agitation, in the additional magnetic field constantly changing of container, magnetic bodies is constantly rotated under variable magnetic force effect, and dilution and sample to be checked are fully mixed, and forms suspension.This alr mode and traditional stirring and compare with spillikin or little rod, have the remarkable advantages such as stirring rate is fast, mixing effect is good.
(5) sampling of the present invention, dilution, magnetic agitation, counting chamber are observed, and speed is fast,! Compare with classic method, there is obvious high flux advantage.
(6) whole experiment testing process is carried out in closed system, and odorlessness, pollution-free is beneficial to laboratory biological protection, meets bio-safety requirement.
Accompanying drawing explanation
Fig. 1 is the structural representation of the sampling rod in the embodiment of the present invention 1.
Fig. 2 is the structural representation of the continuous flow check-out console in the embodiment of the present invention 1.
Fig. 3 is the structural representation of the fixed check-out console in the embodiment of the present invention 2.
In accompanying drawing: 1-sampling rod, 2-blade, 3-center pit, 4-inlet tube, 5-counting chamber, 6-outlet, 7-fixed check-out console, 8-detection cell.
Embodiment
The specific implementation process of the inventive method is described with embodiment below, is explanation of the invention, rather than limitation of the invention, and protection scope of the present invention is not limited only to this.Any improvement of doing according to principle of the present invention, method, change, within including protection scope of the present invention.
embodiment 1
The excretion thing of following employment is example, adopts the metering system of CCD scanning sensor to be described further the inventive method.
(1) take a sampler, as shown in Figure 1, sampler is the sampling rod 1 that a lower end is provided with blade 2, and the blade 2 of sampling rod 1 lower end has a plurality of, in centre, is bound up, and is air vane sheet, mutually between interval form the area of space of fixed size; Sampling rod centre position is provided with a center pit 3, and for adding dilution, center pit 3 is provided with a plurality of outlets in the intersection of blade, and described outlet is for being uniformly distributed.Sampling rod 1 is inserted in people's excretion thing, rotation sampling rod 1, excretion thing is adopted the region between blade.Because the region between the blade 2 of sampling rod 1 lower end is fixed, while being design, through what calculate, so each sample to be checked gathering is a finite quantity, its numerical value is within the scope of design load.In the present embodiment, suppose that the sample to be checked that sampling rod 1 gathers is V1.
(2) get the elliptical vessel that a sectional area is S, the sampling rod 1 that gathers sample to be checked is vertically placed on to elliptical vessel top, the dilution that is V2 by volume again adds in the center pit 3 of sampling rod 1, by the sample to be checked between 3 pairs of sampling rod 1 blades of center pit, rinse, making sample to be checked and volume is that the dilution of V2 joins in elliptical vessel.
(3) dilution and sample to be checked are stirred, form suspension.Described alr mode is magnetic agitation, and the little bar magnet that is V3 by volume is put into elliptical vessel.In the additional magnetic field constantly changing of container, little bar magnet is constantly rotated under variable magnetic force effect again, mixing time is about 1～5 minute, and dilution and sample to be checked are fully mixed, and forms suspension.This alr mode and traditional stirring and compare with spillikin or little rod, have the remarkable advantages such as health, stirring rate is fast, mixing effect is good.
(4) with the height that CCD scanning sensor pair cross-section amasss as the suspension in the elliptical vessel of S, accurately scan.Suppose that the height value that scanning obtains is H, the volume that we just can calculate suspension is so V4=S*H-V3, also just can accurately calculate the volume V1=V4-V2=S*H-V3-V2 of the sample to be detected in container, thereby calculates the concentration of suspension.
(5) get a check-out console, as shown in Figure 2, check-out console is continuous flow check-out console, continuous flow check-out console comprises inlet tube 4, counting chamber 5 and outlet 6, described inlet tube 4 and outlet 6 are located at counting chamber 5 two ends, and be communicated with counting chamber 5, the degree of depth of described counting chamber 5 is H2, suspension is filled with counting chamber 5 by inlet tube.
(6) under the microscope that the counting chamber 5 of filling with suspension to be placed at visual field area be S2, carry out image acquisition analysis, just can know that volume is the quantity of the visible components such as the contained red blood cell of suspension, leucocyte, worm's ovum, mould mycelia or special crystallization of V5=S2*H2, we also just can calculate the quantity of the contained visible component of suspension in unit volume so.According to the concentration of the suspension that calculates in step (4), just can calculate definitely the quantity that does not add contained visible component in the front sample unit volume to be checked of dilution again, thereby provide quantitative testing report accurately.
Above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure, and odorlessness, pollution-free is beneficial to laboratory biological protection, meets bio-safety requirement.
embodiment 2
In the mode of Weighing, the inventive method is further elaborated below.
(1) with a sampler collection people's who limits the quantity of excretion thing, as sample to be checked.
(2) get the container that a weight is G1, the dilution that is G2 by sample to be checked and weight adds in container.
(3) dilution and sample to be checked are stirred, form suspension.Described alr mode is common manual stirring, stirring rod is put into container and constantly stir, and mixing time is about 5 minutes, and dilution and sample to be checked are fully mixed, and forms suspension.
(4) with 100,000/analytical balance, to the container of suspension is housed, accurately weigh, suppose the weight G3 weighing.The weight that just can calculate thus the sample to be detected in container is G4=G3-G2-G1, thereby calculates the concentration of suspension.
(5) get a check-out console, as shown in Figure 3, described check-out console is fixed check-out console 7, and described fixed check-out console 7 middle parts are provided with the detection cell 8 that a known depth is H2, and suspension is filled with to detection cell 8.
(6) detection cell 8 of filling suspension is placed on to the micro-Microscopic observation that a known visual field area is S2, just can know that volume is the quantity of the visible components such as the contained red blood cell of suspension, leucocyte, worm's ovum, mould mycelia or special crystallization of V5=S2*H2, we also just can calculate the quantity of the contained visible component of suspension in unit volume so.According to the concentration of the suspension that calculates in step (4), just can calculate definitely the quantity that does not add contained visible component in the front sample unit weight to be checked of dilution again, thereby provide quantitative testing report accurately.
Above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure, and odorlessness, pollution-free is beneficial to laboratory biological protection, meets bio-safety requirement.
Above two embodiment are explanation of the invention, and non-limiting, and more than just part is given an example.Should be noted that, to one skilled in the art, simple transformation and the improvement according to the ultimate principle of the inventive method and structure, carried out, include within protection scope of the present invention.
Claims (7)
1. visible component High-throughput quantitative analysis method in human or animal's excretion thing, is characterized in that: it comprises the following steps:
By sampler, get human or animal's excretion thing of limiting the quantity of, as sample to be checked;
Get the container of a known volume or weight, quantitative dilution and sample to be checked are joined in this container;
Dilution and sample to be checked are stirred, form suspension;
With surveying instrument to adding volume of a container or the weight of dilution and sample to be checked accurately to measure;
Get a check-out console, check-out console middle part is provided with the counting chamber of a known depth, and suspension is filled with to counting chamber;
The counting chamber of filling with suspension is placed under the microscope of known visual field area and carries out image acquisition analysis.
2. visible component High-throughput quantitative analysis method in human or animal's excretion thing according to claim 1, it is characterized in that: the sampler in described step (1) is that vaned sampling rod is established in a lower end, described blade has a plurality of, in centre, be bound up, be air vane sheet, mutually between interval form the area of space of fixed size, sampling rod is inserted in human or animal's excretion thing, rotation sampling rod, excretion thing is adopted the region between blade.
3. visible component High-throughput quantitative analysis method in human or animal's excretion thing according to claim 1 and 2, it is characterized in that: the stirring in described step (3) is to adopt the mode of magnetic agitation to carry out, first magnetic bodies is added in the container that installs dilution and sample to be checked, again in the additional magnetic field constantly changing of container, magnetic bodies is constantly rotated under variable magnetic force effect, dilution and sample to be checked are fully mixed, form suspension.
4. visible component High-throughput quantitative analysis method in human or animal's excretion thing according to claim 3, it is characterized in that: the surveying instrument described in described step (4) is CCD scanning sensor, with CCD scanning sensor, the suspension volume in container is accurately measured.
5. visible component High-throughput quantitative analysis method in human or animal's excretion thing according to claim 3, is characterized in that: the surveying instrument described in described step (4) is analytical balance, with analytical balance, the container that comprises suspension is carried out to accurate weighing.
6. according to visible component High-throughput quantitative analysis method in the human or animal's excretion thing described in claim 4 or 5, it is characterized in that: the check-out console in described step (5) is continuous flow check-out console, described continuous flow check-out console comprises inlet tube, counting chamber and outlet, described inlet tube and outlet are located at counting chamber two ends, and be communicated with counting chamber, the degree of depth of described counting chamber is known numeric value, and suspension enters counting chamber by inlet tube, carries out under the microscope image acquisition.
7. visible component High-throughput quantitative analysis method in human or animal's excretion thing according to claim 6, is characterized in that: above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310532007.3A CN103645130B (en) | 2013-11-03 | 2013-11-03 | Visible component High-throughput quantitative analysis method in human or animal's excretion thing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310532007.3A CN103645130B (en) | 2013-11-03 | 2013-11-03 | Visible component High-throughput quantitative analysis method in human or animal's excretion thing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103645130A true CN103645130A (en) | 2014-03-19 |
| CN103645130B CN103645130B (en) | 2016-08-17 |
Family
ID=50250381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310532007.3A Active CN103645130B (en) | 2013-11-03 | 2013-11-03 | Visible component High-throughput quantitative analysis method in human or animal's excretion thing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103645130B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104166005A (en) * | 2014-08-20 | 2014-11-26 | 湖南欧杰生物科技发展有限公司 | Full-automatic excrement and secretion detector |
| CN105092283A (en) * | 2015-06-10 | 2015-11-25 | 广州市康立明生物科技有限责任公司 | Sampling rod for fecal sampling device |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5356793A (en) * | 1990-03-15 | 1994-10-18 | Nitta Gelatin Inc. | Method for testing the sensitivity of anticancer drug |
| US5848177A (en) * | 1994-12-29 | 1998-12-08 | Board Of Trustees Operating Michigan State University | Method and system for detection of biological materials using fractal dimensions |
| JP2001074644A (en) * | 1999-09-03 | 2001-03-23 | Agency Of Ind Science & Technol | Device and method for measuring particle in liquid |
| US20050273271A1 (en) * | 2004-04-05 | 2005-12-08 | Aibing Rao | Method of characterizing cell shape |
| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
| CN101637668A (en) * | 2009-01-14 | 2010-02-03 | 中山大学 | Device and method for combined use of molecular imprinting solid phase microextraction and hollow fiber liquid phase microextraction, and application thereof |
| CN102224260A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Kits and devices for detecting analytes |
| CN202939053U (en) * | 2012-10-31 | 2013-05-15 | 屈雅川 | Simple multifunctional disposable stool sampler |
| CN203132980U (en) * | 2013-02-27 | 2013-08-14 | 济南华天恒达科技有限公司 | Medical microscope counting pool |
| CN203259449U (en) * | 2013-04-26 | 2013-10-30 | 江西金洹生物科技有限公司 | Flow counting chamber with variable thickness |
-
2013
- 2013-11-03 CN CN201310532007.3A patent/CN103645130B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5356793A (en) * | 1990-03-15 | 1994-10-18 | Nitta Gelatin Inc. | Method for testing the sensitivity of anticancer drug |
| US5848177A (en) * | 1994-12-29 | 1998-12-08 | Board Of Trustees Operating Michigan State University | Method and system for detection of biological materials using fractal dimensions |
| JP2001074644A (en) * | 1999-09-03 | 2001-03-23 | Agency Of Ind Science & Technol | Device and method for measuring particle in liquid |
| US20050273271A1 (en) * | 2004-04-05 | 2005-12-08 | Aibing Rao | Method of characterizing cell shape |
| CN102224260A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Kits and devices for detecting analytes |
| CN101637668A (en) * | 2009-01-14 | 2010-02-03 | 中山大学 | Device and method for combined use of molecular imprinting solid phase microextraction and hollow fiber liquid phase microextraction, and application thereof |
| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
| CN202939053U (en) * | 2012-10-31 | 2013-05-15 | 屈雅川 | Simple multifunctional disposable stool sampler |
| CN203132980U (en) * | 2013-02-27 | 2013-08-14 | 济南华天恒达科技有限公司 | Medical microscope counting pool |
| CN203259449U (en) * | 2013-04-26 | 2013-10-30 | 江西金洹生物科技有限公司 | Flow counting chamber with variable thickness |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104166005A (en) * | 2014-08-20 | 2014-11-26 | 湖南欧杰生物科技发展有限公司 | Full-automatic excrement and secretion detector |
| CN104166005B (en) * | 2014-08-20 | 2015-09-02 | 湖南欧杰生物科技发展有限公司 | A kind of full-automatic excretion thing detector |
| CN105092283A (en) * | 2015-06-10 | 2015-11-25 | 广州市康立明生物科技有限责任公司 | Sampling rod for fecal sampling device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103645130B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2008209386A5 (en) | ||
| CN103627638B (en) | A kind of composition for splitting erythrocyte, red blood cell lysing agent and application | |
| EP2735859A3 (en) | Method for analyzing blood cells and blood cell analyzer | |
| CN202710548U (en) | Detecting and analyzing meter for platelet aggregation function | |
| CN108303548A (en) | A kind of calibrating method improving c reactive protein testing result consistency | |
| JP2013542442A5 (en) | ||
| CN103645130A (en) | High flux quantitative analysis method for visible components in human or animal excretion | |
| CN112485438A (en) | Specific protein reaction detection method and device | |
| CN108732338A (en) | A kind of classification of compatible leucocyte five and leucocyte three divide the cellanalyzer and test method of group | |
| CN207457252U (en) | A kind of hemoglobin reaction unit and sample analyser | |
| CN101329280A (en) | Fast testing method of hydrogen peroxide residue in milk-like liquid and testing reagent kit thereof | |
| US20210208122A1 (en) | Device and method for analysis of milk | |
| CN203811612U (en) | Automatic analyzer for weighing and quantitative dilution of excrement | |
| CN106442374A (en) | Method for testing content of borax in toy clay material | |
| CN105651718A (en) | Chromometer based fine aggregate methylene blue value rapid determination method | |
| CN205368369U (en) | Bacterium detecting system | |
| CN106053364A (en) | Kit for measuring insulin and preparation method of kit | |
| CN104007113B (en) | The detection method of grain unstrained spirits acidity | |
| CN209181898U (en) | A kind of biological sample Quick measurement tool | |
| CN202814969U (en) | Disposable type consumable for matched handheld type rapid detection analysis device | |
| CN201974353U (en) | Online pressure gradient soil granulometer | |
| CN108956394A (en) | A kind of measurement device of soil mechanical composition | |
| RU88809U1 (en) | DEVICE FOR DETERMINING THE NUMBER OF SOMATIC CELLS IN MILK | |
| CN104122286B (en) | Method for detecting number of rare cells in real time based on low field NMR (nuclear magnetic resonance) | |
| CN217237431U (en) | Sample blending appearance of improvement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wu Baiping Inventor after: Zhang Weihua Inventor before: Ling Tieru Inventor before: Zhang Zhixi Inventor before: Li Zhixiong Inventor before: Zhan Xuan Inventor before: Xie Wenfeng |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |