RU88809U1 - DEVICE FOR DETERMINING THE NUMBER OF SOMATIC CELLS IN MILK - Google Patents

Abstract

1. A device for determining the number of somatic cells in milk, characterized in that it contains a tank for mixing milk with a reagent, made with the possibility of oscillatory movement and consisting of a spherical chamber connected to the outlet pipe, on the open end of which the capillary assembly is fixed, and tubes for pouring milk with a reagent, one end of which is located inside the spherical chamber, and the other end is outside the spherical chamber, a mixing device, on the shaft of which a tank is installed for mixing milk with a reagent and associated with the control and indication device, and a container for accumulating the leaked mixture coming from the capillary assembly, connected through the means of measuring the leaked mixture with the control and indication device. ! 2. The device according to claim 1, characterized in that the tank for mixing milk with the reagent further comprises a tube with a closed end located opposite the outlet pipe. ! 3. The device according to claim 1, characterized in that it further comprises a means for preventing leakage of milk with the reagent during mixing, combined with a tank for mixing milk with the reagent. ! 4. The device according to claim 1, characterized in that the volume of the spherical chamber exceeds the volumes of the mixed milk with the reagent. ! 5. The device according to claim 1, characterized in that the shaft is located horizontally on a straight line with the center of gravity of the tank for mixing milk with the reagent and is rigidly connected with it. ! 6. The device according to claim 5, characterized in that the shaft rotates from the position of the knot during mixing

Description

The utility model relates to the dairy industry and is designed to determine the number of somatic cells in milk by the viscometric method and is used in analytical laboratories, directly at livestock farms, collection centers and / or dairy enterprises when determining the number of somatic cells in milk.

The prior art device for determining the number of somatic cells in milk containing a light guide probe, which from the side of the cell for the test sample ends in a control zone. The output end of the light guide probe is beveled at an angle of 45 °. The paddle mixer and pipette are mounted on the light guide probe from the side of the pointed end of the light guide probe. The ratio of the distances between the paddle mixer and the outlet of the pipette, between the last and sharp ends of the light guide probe and the length of the immersed part is set within 2: 1: 5 9 (RU 2060499, 05.20.1996).

A known somatic cell analyzer, comprising: a flow cell to obtain a milk sample; detection means for identifying the sign of the number of sodium ions in the sample and the product of the ion counting signal whenever the number of somatic cells is higher than expected; control means for receiving an ion counting signal that compares a plurality of qualitative thresholds corresponding to a plurality of qualitative categories and classifying a sample in a qualitative category; and means for determining a number of parameters characterizing a qualitative category (US 6307362, 10.23.2001).

Closest to the proposed utility model is a device for sampling milk, containing a measuring chamber receiving milk samples from the animal, a camera for recording images of milk samples in the measuring part and analysis tool, adapted to count somatic cells and / or complete droplets in the images, filtration means, adapted to ensure the flow of cleaning fluid through the measuring section, after which they take a milk sample, while the camera for recording images records at least one image in the measuring member after, and the analysis tool is adapted to calculate somatic overlap and / or fat in the image in the measuring portion (WO 2008060235, 05.22.2008).

The disadvantage of these devices is the complexity of the designs and inaccuracy of measurements.

The problem to which the proposed utility model is directed is to create such a device for determining the number of somatic cells in milk, which would eliminate the above disadvantages.

The technical result achieved by the implementation of this utility model is to increase the reliability of determining the number of somatic cells in milk, improve service while reducing maintenance costs, improve measurement accuracy, and also expand the functionality of the device and simplify the design of the device.

The specified technical result is achieved in a device for determining the number of somatic cells in milk, containing a reservoir for mixing milk with a reagent, made with the possibility of oscillating movement and consisting of a spherical chamber connected to the outlet pipe, on the open end of which the capillary assembly is fixed, and tubes for pouring milk with a reagent, one end of which is located inside the spherical chamber, and the other end is outside the spherical chamber, a mixing device, on the shaft of which A tank for mixing milk with a reagent and associated with a control and indication device, and a container for accumulating the leaked mixture coming from the capillary assembly, connected through a means for measuring the weight of the leaked mixture with a control and indication device, were installed.

The reservoir for mixing milk with the reagent may additionally contain a tube with a closed end, located opposite the outlet pipe.

Additionally, it may contain means for preventing leakage of milk with the reagent during mixing, combined with a reservoir for mixing milk with the reagent.

The volume of the spherical chamber exceeds the volumes of milk mixed with the reagent.

The shaft can be located horizontally on one straight line with the center of gravity of the tank for mixing milk with the reagent and is rigidly connected with it.

During mixing, the shaft rotates back and forth from the position of the capillary assembly vertically upward at an angle of about ± 90 °, and when the mixture flows out through the capillary assembly, sets the latter vertically downward.

The means for measuring the weight of the leaked mixture is, for example, a weight measuring strain gauge electrically connected to a means for converting information about the weight of the leaked mixture into the number of somatic cells in milk.

The information conversion means is, for example, a microcontroller.

The mixing device consists, for example, of an engine and an optical sensor associated with a control and indication device.

The control and indication device consists, for example, of a liquid crystal display and a processor unit.

The essence of the utility model is illustrated by the drawing, which shows a general diagram of a device for determining the number of somatic cells in milk (by the viscometric method).

A device for determining the number of somatic cells in milk contains:

- a tank 1 for mixing milk with a reagent, configured to perform oscillatory motion. The tank 1 consists of a spherical chamber 2 connected to the outlet pipe 3, on the open end of which the capillary assembly 4 is fixed, and a tube 5 for filling milk with reagent, one end of which is located inside the spherical chamber 2 (in its center) so that the level the mixture during mixing is always below it, and the other end is outside the spherical chamber 2. By its volume, the spherical chamber 2 exceeds the volumes of the milk mixed with the reagent (mixture obtained from them);

- a mixing device 6, on the shaft (not shown) of which a tank 1 for mixing milk with a reagent is installed. The mixing device 6 is connected with the device 7 control and indication;

- a tank 8 for accumulating the leaked mixture coming from the capillary assembly, connected through the leaking mixture measuring means 9 to the control and indication device 7;

- may additionally contain a means (valve) to prevent leakage of milk with the reagent during mixing (not shown), combined with the tank 1 for mixing milk with the reagent.

The tank 1 for mixing milk with the reagent may contain a tube 10 with a closed end located opposite the outlet pipe 3.

The mixing device 6 consists of a motor 11, a disk 12 with a hole, and an optical sensor 13 connected to the control and indication device 7.

The mixing device 6 is implemented using a software-controlled engine 11 (drive), for example, a stepper motor, the shaft 6 of which, for example, is located horizontally on a straight line with the center of gravity of the tank 1 for mixing milk with the reagent and is rigidly connected to it. At the same time, the shaft 6 performs, during mixing, the reverse-rotational movements from the position of the capillary assembly 4 vertically upwards at angles of about ± 90 °, and while the mixture flows out through the capillary assembly 4, sets the latter vertically downward.

The control and indication device 7 consists, for example, of a liquid crystal indicator 14 and a processor unit 15.

Means 9 for measuring the weight of the leaked mixture is, for example, a strain gauge electrically connected to a means for converting information about the weight of the leaked mixture into the number of somatic cells in milk, which is a microcontroller. Means 9 measuring the leaked mixture is located under the tank 8 for accumulation of the leaked mixture coming from the capillary assembly.

The principle of operation of the device for determining the number of somatic cells in milk is based on the measurement of conditional viscosity — the time of expiration of a milk sample through a capillary assembly mixed with a reagent, for example, Mastoprim. Moreover, the outflow time of distilled water is of constant importance and is a calibration and verification parameter for a device for determining the number of somatic cells in milk.

The drug "Mastoprim" destroys the membrane of somatic cells (leukocytes) and DNA molecules pass into the extracellular space, while changing the conditional viscosity in accordance with the calibration table according to GOST 23453-90.

After the Mastoprim preparation is introduced into the spherical chamber through the tube for filling milk with the reagent, the engine of the mixing device is turned on, and the tank for mixing milk with the reagent begins to oscillate. In this case, the shaft of the stirring device performs back and forth rotational movements from the position of the capillary assembly vertically upwards at angles of about ± 90 ° during mixing.

Reproducible wave-like mixing of the entire sample mass ensures its homogeneity, laminar flow through the capillary assembly with an allowable measurement error.

After the mixing process is completed, the shaft slowly sets the capillary assembly vertically downward, and the test mixture (sample) flows out through the capillary assembly and enters the tank for accumulation of the leaked mixture coming from the capillary assembly. After that, through the means for measuring the weight of the leaked mixture (load cell strain gauge), the data is fed to the processor node of the control and indication device and displayed on the liquid crystal display.

Claims (10)

1. A device for determining the number of somatic cells in milk, characterized in that it contains a tank for mixing milk with a reagent, made with the possibility of oscillatory movement and consisting of a spherical chamber connected to the outlet pipe, on the open end of which the capillary assembly is fixed, and tubes for pouring milk with a reagent, one end of which is located inside the spherical chamber, and the other end is outside the spherical chamber, a mixing device, on the shaft of which a tank is installed for mixing milk with a reagent and associated with the control and indication device, and a container for accumulating the leaked mixture coming from the capillary assembly, connected through the means of measuring the leaked mixture with the control and indication device. 2. The device according to claim 1, characterized in that the tank for mixing milk with the reagent further comprises a tube with a closed end located opposite the outlet pipe. 3. The device according to claim 1, characterized in that it further comprises a means for preventing leakage of milk with the reagent during mixing, combined with a tank for mixing milk with the reagent. 4. The device according to claim 1, characterized in that the volume of the spherical chamber exceeds the volumes of the mixed milk with the reagent. 5. The device according to claim 1, characterized in that the shaft is located horizontally on a straight line with the center of gravity of the tank for mixing milk with the reagent and is rigidly connected with it. 6. The device according to claim 5, characterized in that the shaft rotates from the position of the capillary assembly vertically upward through an angle of about ± 90 ° during mixing, and sets the latter vertically downward when the mixture flows out through the capillary assembly. 7. The device according to claim 1, characterized in that the means for measuring the leaked mixture is a load cell strain gauge, electrically connected with the means for converting information about the weight of the leaked mixture into the number of somatic cells in milk. 8. The device according to claim 7, characterized in that the means for converting information is a microcontroller. 9. The device according to claim 1, characterized in that the mixing device consists of an engine and an optical sensor associated with a control and indication device. 10. The device according to claim 9, characterized in that the control and indication device consists of a liquid crystal indicator and a processor unit.