CN103637855A - Goat artificial insemination method - Google Patents

Abstract

The invention discloses a goat artificial insemination method, and relates to the technical field of livestock cultivation. The goat artificial insemination method is characterized by comprising the three steps of selection of rams, preparation before semen collection and semen deposition operation. The steps are very simple and clear, suitable for being operated by professional personnel, also suitable for being operated by non-professional personnel, low in artificial insemination cost, short in cycle, suitable for repeated artificial insemination and long-term insemination work, and high in success rate for the reason that one ewe can be inseminated more than once. The goat artificial insemination method is simple, convenient and fast to operate, and high in insemination efficiency.

Description

A kind of test-tube method of goat

Technical field:

The present invention relates to domestic animal and cultivate technical field, be specifically related to a kind of test-tube method of goat.

Background technology:

Existing more than 150 Goats Breeds in the whole world, can be divided into following classification: milk goat, hair goat, down producing goat, fur goat, meat black goat and common Local Goat.According to the authoritative department incomplete statistics data whole world, raising goat is 10.74 hundred million.Major part is distributed in Asia and Africa country, the nineties is because the level of the productive forces is low, the exploitation of variety source are not also given full play to, along with the continuous growth of national population and the progress of Manufacturing science, people are increasing for the demand of the meat product of high-quality, occurred that the variety source of black goat, in the quality that improves human living standard, particularly provides protein food aspect will obtain sufficient development and utilization.

And the technology of artificial insemination of goat is to collect the seminal fluid of stud ram by the method for artificial vagina, and seminal fluid is carried out to the inspection of quality, and then dilute, with seme-deposition device, be entered into intravaginal a kind of technology of the she-goat that oestruses.

But this technology tends to occur in time deviation, or make a fault in semen collection process, be so just difficult to carry out good artificial insemination, for breeding, just brought certain harm, danger like this.

Summary of the invention:

Technical problem to be solved by this invention is to overcome that existing technological deficiency provides that a kind of method is simple, simple operation, a kind of test-tube method of goat that insemination efficiency is high.

Technical problem to be solved by this invention adopts following technical scheme to realize:

The test-tube method of goat, is characterized in that: comprises the following steps,

The selection of step 1, stud ram:

1.1, meet required kind, the ram age is in 3-4 year, the solid stalwartness of physique, and testicualr development is normal, the stud ram that libido is vigorous especially;

1.2, stud ram need to be looked after and guided before breeding, so that discharge the old essence in body, and strengthens it and produces smart ability, facilitates later semen collection.Concrete method is to carry out 2-3 semen collection every day, each interval 30-40 minute;

Semen collection operation:

2.1, the preparation before semen collection:

2.1.1, the cleaning and sterilizing of semen collection utensil: first, with the dust on appropriate detergent washing semen collection utensil, then rinse well with clear water, then it is put into baking oven, at 110-118 ℃ of sterilization 25-35 minute;

2.1.2, instruction ram: inject the third Testosterone 1-2ml to stud ram, then regularly massage testis with hands, to improve its libido, be convenient to carry out semen collection operation;

2.2, semen collection operation:

2.2.1, semen collection operation: semen collection person squats at stud ram back, when ram is made mounting action, when treating that it stretches out penis, semen collection person should be inserted into its penis in artificial vagina rapidly, when ram has ejaculated, should rapidly artificial vagina be taken out, then take off collection essence bottle;

2.2.2, when ram carries out mounting for the first time, it will be pulled down, and when its mounting for the second time, regather seminal fluid, the quality that so not only can improve seminal fluid can also collect more seminal fluid;

2.3, the processing of seminal fluid:

2.3.1, should avoid sunlight direct projection after seminal fluid collecting, avoid the stimulation of bad smell in environment;

2.3.2, dilution seminal fluid: the diluent of isothermal is gently injected to seminal fluid, shake gently, shake up, be generally doubly advisable with dilution 2-4;

2.3.3, will utilize in time after semen dilution, can not deposit too for a long time, generally surpassing 4 hours motility of sperm will decline, and the conception rate of she-goat will reduce;

2.4, semen quality inspection:

2.4.1, semen volume: the each ejaculum of stud ram is 0.5-1.5ml, and sperm count has hundred million of 20-50, and such requirement is qualified seminal fluid;

2.4.2, color and luster and abnormal smells from the patient: normal semen presents milky, and the concentration of seminal fluid is higher, and milky is heavier, and with sheep smell of mutton;

2.4.3, also to carry out at laboratory the inspection of sperm motility rate and sperm concentration;

Step 3, semen deposition operation:

3.1, the selection of time: the estrus duration of goat is 24-48 hour, ovulation is oestrusing the later stage, therefore semen deposition in 12-17 hour after oestrusing;

3.2, generally an estrus cycle, adopt twice semen deposition, every minor tick 8-12 hour, indivedual longer ewes of estrus duration need to carry out 3 semen depositions;

3.3, Baoding of ewe: between two lower limbs, two handss firmly catch ewe to retreat by the head-clamp of ewe, Baoding does not well allow sheep moving, with stupe, ewe pudendum is cleaned simultaneously, treats semen deposition;

3.4, semen deposition: semen deposition personnel insert 0.4-0.6cm place in cervical orifice by seme-deposition device and carry out semen deposition.After semen deposition, with have gentle hands, pat the afterbody of ewe, make seminal fluid flow into intravaginal completely.

Beneficial effect of the present invention is: very simple and clear of above-mentioned steps, be applicable to professional personnel, also be applicable to non-professional personnel's operation, and test-tube cost is not high, cycle is also shorter, be applicable to repeatedly artificial insemination and long-term insemination work, and an ewe repeatedly inseminates, success rate is just higher like this.The inventive method is simple, simple operation, insemination efficiency are high.

The specific embodiment:

More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and description, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.

Claims (1)

1. the test-tube method of goat, is characterized in that: comprises the following steps,

The selection of step 1, stud ram:

1.1, meet required kind, the ram age is in 3-4 year, the solid stalwartness of physique, and testicualr development is normal, the stud ram that libido is vigorous especially;

1.2, stud ram need to be looked after and guided before breeding, so that discharge the old essence in body, and strengthens it and produces smart ability, facilitates later semen collection.Concrete method is to carry out 2-3 semen collection every day, each interval 30-40 minute;

Semen collection operation:

2.1, the preparation before semen collection:

2.1.1, the cleaning and sterilizing of semen collection utensil: first, with the dust on appropriate detergent washing semen collection utensil, then rinse well with clear water, then it is put into baking oven, at 110-118 ℃ of sterilization 25-35 minute;

2.1.2, instruction ram: inject the third Testosterone 1-2ml to stud ram, then regularly massage testis with hands, to improve its libido, be convenient to carry out semen collection operation;

2.2, semen collection operation:

2.2.1, semen collection operation: semen collection person squats at stud ram back, when ram is made mounting action, when treating that it stretches out penis, semen collection person should be inserted into its penis in artificial vagina rapidly, when ram has ejaculated, should rapidly artificial vagina be taken out, then take off collection essence bottle;

2.2.2, when ram carries out mounting for the first time, it will be pulled down, and when its mounting for the second time, regather seminal fluid, the quality that so not only can improve seminal fluid can also collect more seminal fluid;

2.3, the processing of seminal fluid:

2.3.1, should avoid sunlight direct projection after seminal fluid collecting, avoid the stimulation of bad smell in environment;

2.3.2, dilution seminal fluid: the diluent of isothermal is gently injected to seminal fluid, shake gently, shake up, be generally doubly advisable with dilution 2-4;

2.3.3, will utilize in time after semen dilution, can not deposit too for a long time, generally surpassing 4 hours motility of sperm will decline, and the conception rate of she-goat will reduce;

2.4, semen quality inspection:

2.4.1, semen volume: the each ejaculum of stud ram is 0.5-1.5ml, and sperm count has hundred million of 20-50, and such requirement is qualified seminal fluid;

2.4.2, color and luster and abnormal smells from the patient: normal semen presents milky, and the concentration of seminal fluid is higher, and milky is heavier, and with sheep smell of mutton;

2.4.3, also to carry out at laboratory the inspection of sperm motility rate and sperm concentration;

Step 3, semen deposition operation:

3.1, the selection of time: the estrus duration of goat is 24-48 hour, ovulation is oestrusing the later stage, therefore semen deposition in 12-17 hour after oestrusing;

3.2, generally an estrus cycle, adopt twice semen deposition, every minor tick 8-12 hour, indivedual longer ewes of estrus duration need to carry out 3 semen depositions;

3.3, Baoding of ewe: between two lower limbs, two handss firmly catch ewe to retreat by the head-clamp of ewe, Baoding does not well allow sheep moving, with stupe, ewe pudendum is cleaned simultaneously, treats semen deposition;

3.4, semen deposition: semen deposition personnel insert 0.4-0.6cm place in cervical orifice by seme-deposition device and carry out semen deposition.After semen deposition, with have gentle hands, pat the afterbody of ewe, make seminal fluid flow into intravaginal completely.