CN103621444A - Zebra fish temperature-sensitive behavior track monitor and detection method for effects of cold-heat drug natures of trace ingredients of traditional Chinese medicines - Google Patents
Zebra fish temperature-sensitive behavior track monitor and detection method for effects of cold-heat drug natures of trace ingredients of traditional Chinese medicines Download PDFInfo
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- CN103621444A CN103621444A CN201310634582.4A CN201310634582A CN103621444A CN 103621444 A CN103621444 A CN 103621444A CN 201310634582 A CN201310634582 A CN 201310634582A CN 103621444 A CN103621444 A CN 103621444A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a zebra fish temperature-sensitive behavior track monitor and a detection method for the effects of the cold-heat natures of trace ingredients of traditional Chinese medicines. The monitor comprises a warm water chamber, a normal-temperature water chamber and a cold water chamber, the warm water chamber is isolated from the normal-temperature water chamber by a vertical partition, the normal-temperature water chamber is isolated from the cold water chamber by a vertical partition, the warm water chamber, the normal-temperature water chamber and the cold water chamber are respectively divided into an upper water chamber and a lower water chamber by a horizontal partition, the horizontal partitions in the warm water chamber, the normal-temperature water chamber and the cold water chamber are respectively provided with through holes which communicate the upper water chamber with the lower water chamber, thermostats which can display measured temperature and keep water temperature constant are respectively mounted in the lower water chambers of the warm water chamber, the normal-temperature water chamber and the cold water chamber, the vertical partitions located in the upper water chambers are respectively provided with a notch through which zebra fishes can pass, and the zebra fish temperature-sensitive behavior track monitor also comprises a camera for shooting the behaviors of the zebra fishes. The monitor and the detection method realize the recording of the movement tracks of the zebra fishes and the monitoring of the cold-heat effects of the trace ingredients of traditional Chinese medicines.
Description
technical field
The present invention relates to a kind of zoopery instrument equipment, be specifically related to the temperature sensitive behavior track monitoring of a kind of zebra fish instrument, also relate to a kind of detection method of Chinese medicine micro constitutent fever and chills property of medicine effect.
background technology
There is no at present the temperature sensitive behavior track monitoring of zebra fish instrument, there is no the detection method of Chinese medicine micro constitutent fever and chills property of medicine effect yet.
summary of the invention
The technical problem to be solved in the present invention is to provide the temperature sensitive behavior track monitoring of a kind of zebra fish instrument.
In order to solve the problems of the technologies described above, technical scheme of the present invention is:
The temperature sensitive behavior track monitoring of a kind of zebra fish instrument, comprise warm hydroecium, normal temperature hydroecium and cold and cool hydroecium, warm hydroecium and normal temperature hydroecium are separated by vertical partition plate, normal temperature hydroecium and cold and cool hydroecium are separated by vertical partition plate, warm hydroecium, normal temperature hydroecium and cold and cool hydroecium are divided into upper and lower two-layer hydroecium by horizontal baffle, warm hydroecium, horizontal baffle in normal temperature hydroecium and cold and cool hydroecium all has the through hole of linking up levels hydroecium, warm hydroecium, in lower floor's hydroecium of normal temperature hydroecium and cold and cool hydroecium, be all equiped with and possess observed temperature demonstration and keep the constant thermostat of water temperature, the vertical partition plate that is positioned at upper strata hydroecium all offers the breach passing through for zebra fish, the temperature sensitive behavior track monitoring of this zebra fish instrument also comprises the camera device of taking zebra fish behavior.
The temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument, also comprises for showing and control the control panel of thermostat and camera device.
The temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument, camera device is set up in the top of the temperature sensitive behavior track monitoring of zebra fish instrument by support.
The temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument, the through hole of horizontal baffle is circular hole and semicircle orifice.
The temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument, also comprises the cover plate that covers warm hydroecium, normal temperature hydroecium and cold and cool hydroecium.
The temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument, is equipped with liquid level graduation mark on warm water's locular wall, normal-temperature water locular wall and cold and cool hydroecium wall.
The technical problem to be solved in the present invention is to provide a kind of detection method of Chinese medicine micro constitutent fever and chills property of medicine effect.
In order to solve the problems of the technologies described above, technical scheme of the present invention is:
A kind of detection method of Chinese medicine micro constitutent fever and chills property of medicine effect, to feed micro-cold composition or micro-hot composition or normal zebra fish and put into the temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument and monitor, the video data being obtained by camera device will be carried out to behavior analysis.
The present invention is for studying temperature sensitive behavioural characteristic and the rule thereof of zebra fish, and the cold tendency that warms up under fever and chills physique, pathologic condition, and the impact of the fever and chills property of medicine that can be used for observing Chinese medicine and chemical composition thereof on the temperature sensitive behavior of zebra fish, and then Study of Traditional Chinese Medicine and composition fever and chills property of medicine effect thereof, the material base of the checking Chinese medicine fever and chills property of medicine.The present invention has and makes simple and easy, convenient and practically, has especially realized the movement track that records zebra fish, has realized the monitoring to Chinese medicine micro constitutent fever and chills effect, its visual result, and data analysis is simple, judge objective, conclusion science.
accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is described in more detail:
Fig. 1 is structural representation of the present invention.
In figure: 1 support, 2 warm hydroeciums, 3 vertical partition plates, 4 camera devices, 5 normal temperature hydroeciums, 6 cold and cool hydroeciums, 7 cover plates, 8 liquid level graduation marks, 9 control panels, 10 horizontal baffles, 11 circular holes, 12 semicircle orifices, 13 thermostats.
Embodiment
As shown in Figure 1, the temperature sensitive behavior track monitoring of zebra fish instrument comprises warm hydroecium 2, normal temperature hydroecium 5 and cold and cool hydroecium 6, and warm hydroecium and normal temperature hydroecium are separated by vertical partition plate 3, and normal temperature hydroecium and cold and cool hydroecium are separated by vertical partition plate.Warm hydroecium, normal temperature hydroecium and cold and cool hydroecium are divided into upper and lower two-layer hydroecium by horizontal baffle 10, and the horizontal baffle in warm hydroecium, normal temperature hydroecium and cold and cool hydroecium all has the through hole of linking up levels hydroecium.In lower floor's hydroecium of warm hydroecium, normal temperature hydroecium and cold and cool hydroecium, be all equiped with and possess observed temperature demonstration and keep the constant thermostat 13 of water temperature.The vertical partition plate that is positioned at upper strata hydroecium all offers the breach passing through for zebra fish.The temperature sensitive behavior track monitoring of this zebra fish instrument also comprises the camera device 4 of taking zebra fish behavior, to obtain video data, in order to gather behavior and the trace information thereof of zebra fish.
Adopting zebra fish and do not adopt rat, is because rat can not be observed the fever and chills effect of Chinese medicine micro constitutent, cannot verify the material base of the Chinese medicine fever and chills property of medicine.
Zebra fish fever and chills behavioural trend detector also comprises for showing and control the control panel 9 of thermostat and camera device, to facilitate whole detector real-time operation.
Particularly, camera device is set up in the top of zebra fish fever and chills behavioural trend detector by support 1.
Particularly, the through hole of horizontal baffle is circular hole 11 and semicircle orifice 12.
Zebra fish fever and chills behavioural trend detector also comprises the cover plate 7 that covers warm hydroecium, normal temperature hydroecium and cold and cool hydroecium.
On warm water's locular wall, normal-temperature water locular wall and cold and cool hydroecium wall, be equipped with liquid level graduation mark 8.
A kind of detection method of Chinese medicine micro constitutent fever and chills property of medicine effect, to feed micro-cold composition or micro-hot composition or normal zebra fish puts into the temperature sensitive behavior track monitoring of above-mentioned zebra fish instrument and monitors, to obtain video data by camera device, gather behavior and the trace information thereof of zebra fish, then the video data obtaining is carried out to behavior analysis, to obtain the reflex action rule and characteristic of zebra fish in different temperatures water environment.
Claims (7)
1. the temperature sensitive behavior track monitoring of a zebra fish instrument, it is characterized in that: comprise warm hydroecium, normal temperature hydroecium and cold and cool hydroecium, warm hydroecium and normal temperature hydroecium are separated by vertical partition plate, normal temperature hydroecium and cold and cool hydroecium are separated by vertical partition plate, warm hydroecium, normal temperature hydroecium and cold and cool hydroecium are divided into upper and lower two-layer hydroecium by horizontal baffle, warm hydroecium, horizontal baffle in normal temperature hydroecium and cold and cool hydroecium all has the through hole of linking up levels hydroecium, warm hydroecium, in lower floor's hydroecium of normal temperature hydroecium and cold and cool hydroecium, be all equiped with and possess observed temperature demonstration and keep the constant thermostat of water temperature, the vertical partition plate that is positioned at upper strata hydroecium all offers the breach passing through for zebra fish, the temperature sensitive behavior track monitoring of this zebra fish instrument also comprises the camera device of taking zebra fish behavior.
2. the temperature sensitive behavior track monitoring of zebra fish according to claim 1 instrument, is characterized in that: also comprise for showing and control the control panel of thermostat and camera device.
3. the temperature sensitive behavior track monitoring of zebra fish according to claim 1 instrument, is characterized in that: camera device is set up in the top of the temperature sensitive behavior track monitoring of zebra fish instrument by support.
4. the temperature sensitive behavior track monitoring of zebra fish according to claim 1 instrument, is characterized in that: the through hole of horizontal baffle is circular hole and semicircle orifice.
5. the temperature sensitive behavior track monitoring of zebra fish according to claim 1 instrument, is characterized in that: also comprise the cover plate that covers warm hydroecium, normal temperature hydroecium and cold and cool hydroecium.
6. the temperature sensitive behavior track monitoring of zebra fish according to claim 1 instrument, is characterized in that: on warm water's locular wall, normal-temperature water locular wall and cold and cool hydroecium wall, be equipped with liquid level graduation mark.
7. the detection method of a Chinese medicine micro constitutent fever and chills property of medicine effect, it is characterized in that: by feeding micro-cold composition or micro-hot composition or normal zebra fish, put into the arbitrary described temperature sensitive behavior track monitoring of the zebra fish instrument of claim 1-6 and monitor, the video data being obtained by camera device is carried out to behavior analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310634582.4A CN103621444A (en) | 2013-12-03 | 2013-12-03 | Zebra fish temperature-sensitive behavior track monitor and detection method for effects of cold-heat drug natures of trace ingredients of traditional Chinese medicines |
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| CN201310634582.4A CN103621444A (en) | 2013-12-03 | 2013-12-03 | Zebra fish temperature-sensitive behavior track monitor and detection method for effects of cold-heat drug natures of trace ingredients of traditional Chinese medicines |
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| CN103621444A true CN103621444A (en) | 2014-03-12 |
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Cited By (3)
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| CN104221970A (en) * | 2014-09-25 | 2014-12-24 | 山东省科学院生物研究所 | Device for researching learning and memory ability of zebra fish |
| CN105796109A (en) * | 2016-03-08 | 2016-07-27 | 山东中医药大学 | Mouse coldness and heat approach behavior tester |
| CN106719415A (en) * | 2016-12-27 | 2017-05-31 | 浙江海洋大学 | A kind of detection means and method for judging yellow volume box tortoise learning ability |
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| CN104221970B (en) * | 2014-09-25 | 2016-08-24 | 山东省科学院生物研究所 | A kind of device for studying Brachydanio rerio ability of learning and memory |
| CN105796109A (en) * | 2016-03-08 | 2016-07-27 | 山东中医药大学 | Mouse coldness and heat approach behavior tester |
| CN106719415A (en) * | 2016-12-27 | 2017-05-31 | 浙江海洋大学 | A kind of detection means and method for judging yellow volume box tortoise learning ability |
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Application publication date: 20140312 |