CN103614434B - A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide - Google Patents
A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide Download PDFInfo
- Publication number
- CN103614434B CN103614434B CN201310445200.3A CN201310445200A CN103614434B CN 103614434 B CN103614434 B CN 103614434B CN 201310445200 A CN201310445200 A CN 201310445200A CN 103614434 B CN103614434 B CN 103614434B
- Authority
- CN
- China
- Prior art keywords
- herba eichhorniae
- oligosaccharide
- xylo
- add
- potassium hydroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a kind of Herba Eichhorniae and extract the method preparing xylo-oligosaccharide, comprise the following steps: the pre-treatment of Herba Eichhorniae; The extraction of xylo-oligosaccharide.The present invention has the following advantages: take full advantage of Herba Eichhorniae resource, turn waste into wealth, and solves the problem of environmental pollution that Herba Eichhorniae too much causes; The method preparation technology is simple, easy handling, easy suitability for industrialized production; It is high that the present invention extracts xylo-oligosaccharide yield, has been up to 7.8%.
Description
Technical field
The present invention relates to xylo-oligosaccharide preparation field, be specifically related to a kind of Herba Eichhorniae and extract the method preparing xylo-oligosaccharide.
Background technology
Herba Eichhorniae itself has very strong ability of purifying waste water, but a large amount of Herba Eichhorniaes covers river surface, easily causes water quality deterioration, affects the growth of benthos.Herba Eichhorniae reproduction speed is exceedingly fast, and can consume a large amount of dissolved oxygen during growth, has almost become the synonym of " pollution "., all there is the situation that Herba Eichhorniae overflows, and expended huge fund and also cannot effect a radical cure in the famous water bodys such as Dian Chi, Taihu Lake, Huangpu River and Wuhan East Lake.Herba Eichhorniae is one of classical case of invasive species harm to the damnous case in Dian Chi.The eighties in 20th century, the desirable water tourism route of grand sight river-Dian Chi-Western Hills has been built up in Kunming, and visitor can go for a sail Dian Chi, Western Hills from city.But the beginning of the nineties, Herba Eichhorniae overgrowing in grand sight river and Dian Chi is caused disaster, and covers the water surface of whole river surface and part Dian Chi, causes this tourism route to be forced to cancel, the supporting Tourist facilities built in two sides, grand sight river have to be discarded or change and do other purposes, and subterranean stream is also reconstructed in grand sight river.These are direct financial loss, and the Ecological Loss caused by Herba Eichhorniae is but difficult to estimate.
On the one hand, for all events of excessive Herba Eichhorniae environmental pollution, on the other hand, there is complex process in the extraction of xylo-oligosaccharide, the problem that extraction yield is extremely low.
Summary of the invention
Goal of the invention: the object of the invention is a kind of Herba Eichhorniae and extract the method preparing xylo-oligosaccharide, not only Herba Eichhorniae is turned waste into wealth, and the extraction process of this xylo-oligosaccharide is simple, output is high.
Technical scheme: in order to solve the problems of the technologies described above, the invention provides a kind of Herba Eichhorniae and extracts the method preparing xylo-oligosaccharide, comprise the following steps:
1) pre-treatment of Herba Eichhorniae: collection Herba Eichhorniae is placed in electric heating revolution steamer, add alkali, boiling temperature is 100 ~ 150 DEG C, and cooking time is that 1 ~ 3h obtains extracting solution.
2) extraction of xylo-oligosaccharide: be put into whizzer centrifugal 10 ~ 15min under 10000 ~ 12000r/m rotating speed after 2 ~ 3 to obtain supernatant liquor by adding acid for adjusting pH in extracting solution, by supernatant liquor with 3% potassium hydroxide solution regulate pH to be neutrality after, add xylanase on shaking table, to carry out enzymolysis obtain mixed solution, centrifugal rear supernatant is xylo-oligosaccharide.
Alkali in described step 1) is potassium hydroxide, and the add-on of described potassium hydroxide accounts for 2% ~ 10% of Herba Eichhorniae weight.
Described acid is formic acid.
Described step 2) in the add-on of xylanase be 100 ~ 600U/ml, hydrolysis temperature is 50 ~ 60 DEG C, and enzymolysis time is 10 ~ 15h, and shaking speed is 100 ~ 150r/min.
Described step 2) after enzymolysis, 3ml trichloromethane will be added in mixed solution before centrifugal, and boil at 100 DEG C.
Beneficial effect: the present invention has the following advantages:
1) take full advantage of Herba Eichhorniae resource, turn waste into wealth, solve the problem of environmental pollution that Herba Eichhorniae too much causes.
2) the method preparation technology is simple, easy handling, easy suitability for industrialized production.
3) to extract xylo-oligosaccharide yield high in the present invention, has been up to 7.8%.
embodiment:
Embodiment 1:
Herba Eichhorniae extracts the method preparing xylo-oligosaccharide, comprises the following steps:
1) pre-treatment of Herba Eichhorniae: collection Herba Eichhorniae is placed in electric heating revolution steamer, add potassium hydroxide, the add-on of described potassium hydroxide accounts for 2% of Herba Eichhorniae weight, and boiling temperature is 100 DEG C, and cooking time is that 1h obtains extracting solution.
2) extraction of xylo-oligosaccharide: be put into whizzer centrifugal 15min under 10000r/m rotating speed after 2 to obtain supernatant liquor by adding first acid for adjusting pH in extracting solution, by supernatant liquor with 3% potassium hydroxide solution regulate pH to be neutrality after, add xylanase on shaking table, to carry out enzymolysis obtain mixed solution, the add-on of described xylanase is 600U/ml, hydrolysis temperature is 50 DEG C, enzymolysis time is 10h, shaking speed is 100r/min, after enzymolysis, 3ml trichloromethane will be added in mixed solution, and boil at 100 DEG C, centrifugal rear supernatant is xylo-oligosaccharide.The analysis of xylo-oligosaccharide solution draws, poly-pentose yield is 7.2%.
Embodiment 2
Herba Eichhorniae extracts the method preparing xylo-oligosaccharide, comprises the following steps:
1) pre-treatment of Herba Eichhorniae: collection Herba Eichhorniae is placed in electric heating revolution steamer, add potassium hydroxide, the add-on of described potassium hydroxide accounts for 10% of Herba Eichhorniae weight, and boiling temperature is 150 DEG C, and cooking time is that 3h obtains extracting solution.
2) extraction of xylo-oligosaccharide: be put into whizzer centrifugal 10min under 12000r/m rotating speed after 3 to obtain supernatant liquor by adding first acid for adjusting pH in extracting solution, by supernatant liquor with 3% potassium hydroxide solution regulate pH to be neutrality after, add xylanase on shaking table, to carry out enzymolysis obtain mixed solution, the add-on of described xylanase is 100U/ml, hydrolysis temperature is 60 DEG C, enzymolysis time is 15h, shaking speed is 150r/min, after enzymolysis, 3ml trichloromethane will be added in mixed solution, and boil at 100 DEG C, centrifugal rear supernatant is xylo-oligosaccharide.The analysis of xylo-oligosaccharide solution draws, poly-pentose yield is 7.3%.
Embodiment 3
Herba Eichhorniae extracts the method preparing xylo-oligosaccharide, comprises the following steps:
1) pre-treatment of Herba Eichhorniae: collection Herba Eichhorniae is placed in electric heating revolution steamer, add potassium hydroxide, the add-on of described potassium hydroxide accounts for 6% of Herba Eichhorniae weight, and boiling temperature is 130 DEG C, and cooking time is that 2h obtains extracting solution.
2) extraction of xylo-oligosaccharide: be put into whizzer centrifugal 13min under 11000r/m rotating speed after 2.5 to obtain supernatant liquor by adding first acid for adjusting pH in extracting solution, by supernatant liquor with 3% potassium hydroxide solution regulate pH to be neutrality after, add xylanase on shaking table, to carry out enzymolysis obtain mixed solution, the add-on of described xylanase is 350U/ml, hydrolysis temperature is 55 DEG C, enzymolysis time is 13h, shaking speed is 175r/min, after enzymolysis, 3ml trichloromethane will be added in mixed solution, and boil at 100 DEG C, centrifugal rear supernatant is xylo-oligosaccharide.The analysis of xylo-oligosaccharide solution draws, poly-pentose yield is 7.8%.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. Herba Eichhorniae extracts the method preparing xylo-oligosaccharide, it is characterized in that, comprises the following steps:
1) pre-treatment of Herba Eichhorniae: collection Herba Eichhorniae is placed in electric heating revolution steamer, add alkali, boiling temperature is 100 ~ 150 DEG C, and cooking time is that 1 ~ 3h obtains extracting solution;
2) extraction of xylo-oligosaccharide: adding acid for adjusting pH in extracting solution is put into whizzer centrifugal 10 ~ 15min under 10000 ~ 12000r/min rotating speed after 2 ~ 3 to obtain supernatant liquor, by supernatant liquor with 3% potassium hydroxide solution regulate pH to be neutrality after, add xylanase on shaking table, to carry out enzymolysis obtain mixed solution, centrifugal rear supernatant is xylo-oligosaccharide;
Alkali in described step 1) is potassium hydroxide, and the add-on of described potassium hydroxide accounts for 2% ~ 10% of Herba Eichhorniae weight;
Described step 2) in the add-on of xylanase be 100 ~ 600U/ml, hydrolysis temperature is 50 ~ 60 DEG C, and enzymolysis time is 10 ~ 15h, and shaking speed is 100 ~ 150r/min; Described step 2) in acid be formic acid;
Described step 2) after enzymolysis, in mixed solution, add 3ml trichloromethane before centrifugal, and boil at 100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310445200.3A CN103614434B (en) | 2013-09-27 | 2013-09-27 | A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310445200.3A CN103614434B (en) | 2013-09-27 | 2013-09-27 | A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103614434A CN103614434A (en) | 2014-03-05 |
| CN103614434B true CN103614434B (en) | 2016-03-23 |
Family
ID=50165149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310445200.3A Expired - Fee Related CN103614434B (en) | 2013-09-27 | 2013-09-27 | A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103614434B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646843B (en) * | 2020-12-31 | 2022-03-08 | 安徽丰原发酵技术工程研究有限公司 | Method for preparing lactic acid by using water hyacinth as raw material |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102076741A (en) * | 2008-04-30 | 2011-05-25 | 希乐克公司 | biomass processing |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI376419B (en) * | 2009-10-22 | 2012-11-11 | Atomic Energy Council | Method for improving the efficiency of xylose fermentation in lignocellulosic hydrolysate |
-
2013
- 2013-09-27 CN CN201310445200.3A patent/CN103614434B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102076741A (en) * | 2008-04-30 | 2011-05-25 | 希乐克公司 | biomass processing |
Non-Patent Citations (3)
| Title |
|---|
| Saccharification of agro-wastes by endoxylanase from Streptomyces sp om 09;Rina Rani Ray;《International Journal of life Sciences Biotechnology and Pharma Research》;20130430;第2卷(第2期);摘要，第165页左栏第1段至右栏第1段，第166页右栏第2-4段，第167页左栏第1段，第168页右栏第2段至第169页左栏第1段，表1，图4 * |
| 低聚木糖的研究进展;谢全喜等;《畜牧与饲料科学》;20120630;第33卷(第5-6期);摘要，第30页左栏第1段至第32页右栏第5段 * |
| 农作物秸秆的综合利用;韩学凤等;《北京农学院学报》;20030930;第18卷(第3期);摘要，第226页第1、4段，第227页第4-5段，第228页第5段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103614434A (en) | 2014-03-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104561190B (en) | A kind of two-step method method of utilizing bamboo biomass waste enzyme hydrolysis production fermentable sugars | |
| WO2009155675A3 (en) | Process for the recovery of water and energy from the processing of sugar cane in sugar and ethanol production mills | |
| MY176473A (en) | Method of producing sugar liquid | |
| CN104072370A (en) | Method for purifying cynarin by microwave and ultrasonic combination method | |
| CN103665195B (en) | A kind of method utilizing agar in Enzymatic Extraction fragrant plant mentioned in ancient texts | |
| CN204466467U (en) | A kind of irrigation system utilizing solar energy | |
| CN101597339A (en) | A kind of is the method for feedstock production tremella polysaccharide with white fungus, defective goods and ear pin | |
| CN103614434B (en) | A kind of Herba Eichhorniae extracts the method preparing xylo-oligosaccharide | |
| CN104479033A (en) | Method for comprehensive separation extraction of glycyrrhizic acid, licoflavone and glycyrrhiza polysaccharide from licorice root | |
| CN103305564B (en) | Method for converting icariin to herba epimedii | |
| CN104072554A (en) | Method for extracting paederia scandens from fevervine | |
| CN102321125B (en) | Process for extracting gallotannic acid from galla chinensis | |
| Ganguly et al. | Effect of alkali preteatment on water hyacinth biomass for production of ethanol | |
| CN103485232B (en) | A kind of utilize water hyacinth obtained paper and manufacture method | |
| CN103707551B (en) | Cycles, economized hot press | |
| CN103626883A (en) | Extraction method for hedyotis diffusa polysaccharide | |
| CN108623710A (en) | A kind of method of microwave-enzyme assisted extraction bagasse hemicellulose | |
| CN102558381B (en) | Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism | |
| CN102660619A (en) | Method for clean production of saponin from Dioscorea zingiberensis C. H. Wright | |
| CN202047262U (en) | Steam recycling system for production | |
| CN104313106B (en) | Method for preparing ethanol by using binary regulated aureobasidium pullulans and pichia pastoris fermented reed | |
| CN101245112B (en) | Polyoses extracted from pistacia chinensis meal and extracting method thereof | |
| CN106086086A (en) | A kind of reduce the method for formic acid concn in bagasse hydrolyzate | |
| CN203323589U (en) | Device for recycling condensate water produced in chinlon production process | |
| CN203403275U (en) | Dyeing condensate water and cooling water recycling system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160323 Termination date: 20200927 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |