CN103613677A - Extraction method of imperatoria polysaccharide - Google Patents
Extraction method of imperatoria polysaccharide Download PDFInfo
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- CN103613677A CN103613677A CN201310578245.8A CN201310578245A CN103613677A CN 103613677 A CN103613677 A CN 103613677A CN 201310578245 A CN201310578245 A CN 201310578245A CN 103613677 A CN103613677 A CN 103613677A
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Abstract
The invention discloses an extraction method of imperatoria polysaccharide. The extraction method comprises the following steps: drying and crushing imperatoria; adding distilled water and imperatoria powder according to a certain liquid-material ratio into a water bath for extraction and filtration; metering the volume of a filtrate; by taking glucose standard substance liquor as reference and adopting a phonel-sulfate method to measure the content of crude polysaccharide, and carrying out ethanol precipitation and acetone washing to the extraction liquor, thus finally obtaining the imperatoria polysaccharide powder. The extraction method disclosed by the invention has the specific beneficial effects that by considering influence of various factors on polysaccharide extraction and adopting an orthogonal experimental method to strictly control proportion of an extracting agent water and imperatoria powder, extraction duration, extraction time and extraction temperature in the extraction process, the whole extraction process is simple to operate, simplified in program, accurate in extraction and good in repeatability, and the extraction rate of imperatoria polysaccharide is improved.
Description
Technical field
The present invention relates to the extracting method with a kind of imperatoria chemical composition, relate to specifically a kind of extracting method of imperatoria polysaccharide.
Background technology
Imperatoria (Latin formal name used at school Pennisetum purpureum K.Scbumacb, P.typhoideum Rich), Chinese another name emperor grass (English another name Chinese sugarcane, japanese cane, king grass, north Indian sugarcane, uva cane), for Poales Gramineae Pennisetum plant, it is a kind of good tropical pasture, extensively cultivate in domestic Hainan, Guangdong, Guangxi, Jiangsu, Fujian, Yunnan, Hunan introducing culture.In being abroad mainly distributed in, South America.Perennial growing thickly property high strawed rice crop grass.Well developed root system.Cane likeness in form sugarcane.Leaf is elongated.The close life of panicle is spike.Caryopsis fusiform, light yellow, glossy.The weather condition of happiness warm and moist, not cold-resistant, drought-resistant, fire-resistant burning, strong stress resistance.Feeding value is higher, and general using method is for cradling rear Direct-fed animal or being modulated into silage.
Vegetable polysaccharides has multiple bioactive functions, comprises immunomodulatory, antitumor, hypoglycemic, reducing blood-fat etc.Imperatoria contains abundant polysaccharide component, extracts polysaccharide from imperatoria, to widening imperatoria, utilizes the economic benefit of channel and raising plantation imperatoria significant.At present, it is reported and retrieve, about the vegetable polysaccharides of imperatoria, extracting and not yet have relevant report and document to record.
Summary of the invention
The deficiency existing in order to overcome above technology, the present invention aims to provide a kind of extracting method of imperatoria polysaccharide.
The technical scheme that technical solution problem of the present invention adopts is: a kind of extracting method of imperatoria polysaccharide, the method comprises the following steps:
(1) the imperatoria raw material of fresh imperatoria cauline leaf is cut into segment, in baking oven, toasts and be dried to constant weight in 46～50 hours, then with pulverizer, be ground into powder, excessively standby after 30～50 mesh sieves;
(2) in imperatoria powder, add the distilled water of 10～30 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 60～90 ℃, extraction time is 20～50 minutes, repeats said extracted process 1～3 time, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution;
(4) in extracting solution, add 95% ethanolic soln and shake up, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with acetone, fully wash, again filter or centrifugal, the product obtaining is imperatoria polysaccharide after lyophilize.
Preferably, the extracting method of described a kind of imperatoria polysaccharide, is characterized in that:
(1) described imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 15 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 80 ℃, extraction time is 30 minutes, repeats said extracted process 2 times, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution;
(4) by with extracting liquid volume than adding 95% ethanolic soln and shake up for 1:1, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with 2 times of volume acetone of throw out, fully wash, filtration or centrifugal again, the throw out obtaining is imperatoria polysaccharide after lyophilize.
The concrete beneficial effect of the present invention is: considered the impact that many factors extracts polysaccharide, adopt orthogonal test method strictly to control the ratio of extraction agent water in leaching process and imperatoria powder, extraction time, extraction time and extraction temperature, the extraction yield that whole leaching process is simple to operate, program simplification, extraction are accurate, reproducible, improved imperatoria polysaccharide.
Concrete embodiment
Embodiment mono-
An extracting method for imperatoria polysaccharide, the method comprises the following steps:
(1) imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 15 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 80 ℃, extraction time is 30 minutes, repeats said extracted process 2 times, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution, in the present embodiment, polysaccharide content is 9.20%;
(4) by with extracting liquid volume than adding 95% ethanolic soln and shake up for 1:1, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with 2 times of volume acetone of throw out, fully wash, filtration or centrifugal again, the throw out obtaining is imperatoria polysaccharide after lyophilize.
Embodiment bis-
An extracting method for imperatoria polysaccharide, the method comprises the following steps:
(1) imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 20 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 70 ℃, extraction time is 20 minutes, repeats said extracted process 1 time, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution, in the present embodiment, polysaccharide content is 6.32%;
(4) by with extracting liquid volume than adding 95% ethanolic soln and shake up for 1:1, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with 2 times of volume acetone of throw out, fully wash, filtration or centrifugal again, the throw out obtaining is imperatoria polysaccharide after lyophilize.
Embodiment tri-
An extracting method for imperatoria polysaccharide, the method comprises the following steps:
(1) imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 25 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 90 ℃, extraction time is 40 minutes, repeats said extracted process 3 times, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution, in the present embodiment, polysaccharide content is 6.78%;
(4) by with extracting liquid volume than adding 95% ethanolic soln and shake up for 1:1, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with 2 times of volume acetone of throw out, fully wash, filtration or centrifugal again, the throw out obtaining is imperatoria polysaccharide after lyophilize.
Above disclosed is only preferred embodiment of the present invention, certainly can not limit with this interest field of the present invention, and the equivalent variations of therefore doing according to the claims in the present invention, still belongs to the scope that the present invention is contained.
Claims (2)
1. an extracting method for imperatoria polysaccharide, the method comprises the following steps:
(1) imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 15～25 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 70～90 ℃, extraction time is 20～40 minutes, repeats said extracted process 1～3 time, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution;
(4) in extracting solution, add 95% ethanolic soln and shake up, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with acetone, fully wash, again filter or centrifugal, the product obtaining is imperatoria polysaccharide after lyophilize.
2. the extracting method of a kind of imperatoria polysaccharide according to claim 1, is characterized in that:
(1) described imperatoria raw material is fresh imperatoria cauline leaf, is cut into about 3cm segment, in 65 ℃ of baking ovens, toasts and is dried to constant weight in 48 hours, then with pulverizer, is ground into powder, excessively standby after 40 mesh sieves;
(2) in imperatoria powder, add the distilled water of 15 times of weight to pour in round-bottomed flask container, putting into thermostat water bath at the uniform velocity vibrates, extracting temperature is 80 ℃, extraction time is 30 minutes, repeats said extracted process 2 times, treats that solution is cooling after having extracted, double-deck middling speed qualitative filter paper filters, distilled water flushing filter residue, merging filtrate constant volume, to be measured;
(3) take dextrose standard sample solution as contrast, adopt phenol sulfuric acid process to measure the content of polysaccharide in extracting solution;
(4) by with extracting liquid volume than adding 95% ethanolic soln and shake up for 1:1, in 4 ℃ of refrigerators standing 24 hours, after filtration or after centrifugal, be precipitated thing, with 2 times of volume acetone of throw out, fully wash, filtration or centrifugal again, the throw out obtaining is imperatoria polysaccharide after lyophilize.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757045A (en) * | 2008-11-25 | 2010-06-30 | 天津太平洋制药有限公司 | Preparation method of pachyman |
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
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- 2013-11-18 CN CN201310578245.8A patent/CN103613677A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757045A (en) * | 2008-11-25 | 2010-06-30 | 天津太平洋制药有限公司 | Preparation method of pachyman |
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
Non-Patent Citations (3)
| Title |
|---|
| 刘昔辉 等: "甘蔗叶多糖的提取与含量测定", 《安徽农业科学》, vol. 35, no. 34, 31 December 2007 (2007-12-31), pages 10960 - 11035 * |
| 吴玉强 等: "多指标正交优选甘蔗叶多糖的提取工艺", 《中国实验方剂学杂志》, vol. 17, no. 19, 31 October 2011 (2011-10-31), pages 11 - 13 * |
| 桂意云 等: "甘蔗叶多糖的提取及体外抗氧化作用", 《西南农业学报》, vol. 25, no. 4, 31 December 2012 (2012-12-31), pages 1218 - 1221 * |
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Application publication date: 20140305 |