CN103609595A - Biological inoculant for cotton growth promotion, premature senility resistance and boll shedding resistance of cotton, and preparation method and application thereof - Google Patents
Biological inoculant for cotton growth promotion, premature senility resistance and boll shedding resistance of cotton, and preparation method and application thereof Download PDFInfo
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- CN103609595A CN103609595A CN201310515077.8A CN201310515077A CN103609595A CN 103609595 A CN103609595 A CN 103609595A CN 201310515077 A CN201310515077 A CN 201310515077A CN 103609595 A CN103609595 A CN 103609595A
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Abstract
The invention provides a biological inoculant for growth promotion, premature senility resistance and boll shedding resistance of cotton. The biological inoculant comprises the following materials: a bacterial strain ACCC30352, a bacterial strain ACCC30334 and a bacterial strain ACCC31052. The biological inoculant provided by the invention generates secondary compounds, which can promote the growth of cotton, solve the problem of slow growth of cotton seedling at early stage, resist premature senility of cotton, enhance stress resistance and disease resistance of cotton, as well as protect flower and bud bolls, prevent shedding of cotton bolls and substantially increase the yield of cotton. The biological inoculant provided by the invention can be continuously sprayed (dripped) since seedling emergence of cotton every other 7-10 days, wherein obvious effects can be shown for continuous usage for more than 5 times. The biological inoculant provided by the invention is simple for usage, convenient, low-cost, safe and environment-friendly, and has high cost performance.
Description
Technical field
The invention belongs to microbial bacterial agent technical field, be specifically related to a kind of bacteria agent for cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls.
Background technology
Xinjiang region cotton in seedling stage is because temperature is lower, cause poor growth, the way that general employing sprays gibberellin continuously solves this problem, but because the use of gibberellin can cause cell transition to elongate the rear early ageing phenomenon producing, at cotton knot a large amount of fruit abscission rate of bell phase, can make cotton Severe Reduction more than 30%.The leader of the Nong Liu teacher institute of agricultural sciences and scientific research personnel are studying always and are exploring for many years biotechnology and product and solving this problem.
Summary of the invention
In order to solve problems of the prior art, the invention provides a kind of bacteria agent, can effectively solve Xinjiang region cotton growth slowly, early ageing and in the problem of cotton knot bell phase fruit abscission rate, for this reason, the present invention also provides preparation method and its application in cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls of this bacteria agent.
The invention provides a kind of bacteria agent for cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls, described bacteria agent comprises following material: strains A CCC30352, strains A CCC30334 and strains A CCC31052.
Above-mentioned three kinds of combinations of substances are together time, because the characteristic of three kinds of bacterial strains can be used in cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls.
Preferably, in described bacteria agent, the mass ratio of strains A CCC30352, strains A CCC30334 and strains A CCC31052 is 1:1:1.
When above-mentioned three kinds of bacterial strain mass ratioes are 1:1:1, better to cotton growth promotion, premature senescence resistance, anti-shedding of cotton bolls effect.
Preferably, described bacteria agent is comprised of each component of following quality percentage composition: the zymotic fluid 20% of strains A CCC30352, the zymotic fluid 20% of strains A CCC30334, the zymotic fluid 20% of strains A CCC31052, oxyenadenine 0.3%, isopentennyladenine 0.3%, humic acid potassium or nitro khm 10%, potassium alginate 10%, pH value is adjusted to 6.0, and surplus is water.
The three kinds of bacterial strains of take are active component, are aided with the bacteria agent that various auxiliary materials are made according to the above ratio, and the effect of cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls is reached to best.
Second object of the present invention is to provide the preparation method of above-mentioned bacteria agent, is each component of formula ratio is mixed, and adjust pH to 6.0 obtains.
Preferably, the preparation method of the zymotic fluid of described strains A CCC30352 is: strains A CCC30352 is accessed in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, access in the fermentation tank that sterilizing cooled liquid fermentation medium are housed and carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput starts at 1:1.2, cultivate and increase to 1:1.5 after 24 hours, after 48 hours, be down to 1:1, fermentation is complete to fermentation in 72 hours, when the bacterium amount of ACCC30352 reaches hundred million/ml of 20-30 in process microscopy zymotic fluid, obtain zymotic fluid.
Preferably, the preparation method of the zymotic fluid of described strains A CCC30334 and ACCC31052 is: bacterial strain is accessed respectively in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, access in the fermentation tank that sterilizing cooled liquid fermentation medium are housed and carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput starts at 1:1, cultivate and increase to 1:1.2 after 24 hours, after 48 hours, continue to increase to 1:1.5; Fermentation to 72 hour fermentation is complete, when bacterium amount reaches hundred million/ml of 20-30 in process microscopy zymotic fluid, obtains zymotic fluid.
Preferably, the preparation method of described liquid fermentation medium is: mass percent 20% potato juice-1000ml, glucose-20g, KH
2pO
4-3g, MgSO
47H
2o-1.5g, thiamine-0.05g, oxyenadenine-0.3g, isopentennyladenine-0.3g, xanthans-3g, agar-5g, mixes above-mentioned each component, and pH value is adjusted to 6.0.
The 3rd object of the present invention is to provide the application of above-mentioned bacteria agent in cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls.
Preferably, described application is to adopt the method for drip irrigation that bacteria agent is applied to cotton root, from seedling stage to the cotton growth later stage, every about 7-10 days, uses once.
Preferably, the whole process of growth in cotton does not need to spray gibberellin again.
The present invention is with 3 strains A CCC30352: Fusorium moniliforme Sheldon
fusarium monilifformesheldon, ACCC30334: Fusorium moniliforme Sheldon
fusarium monilifformesheldon and ACCC31052: Gibberella zeae
gibberella zeae(Schw.) these 3 bacterial classifications of Petch(all obtain and are purchased from Chinese agriculture microorganism fungus kind preservation administrative center) zymotic fluid as the active component of cotton growth promotion, premature senescence resistance, slip-off preventing bacteria agent, be aided with natural carrier material (humic acid potassium: humic acid potassium possesses rich carboxyl, hydroxyl, phenolic hydroxyl group, quinonyl, methoxyl group isoreactivity group, apply after earth, can react through complexing, chelating, suction-operated and earth mineral, improvement clay structure, activation earth nutrient, fertilizer conservation, the water retention capacity of raising earth.Improved effect to low-yield land, acid soil, white muddy soil, saline land, husky alkali ground, sandy bare land is obvious, more outstanding is the content that can improve earth available potassium (particularly water-soluble potassium), in minimizing earth, potassium element is fixing, promote the release of slightly solubility potassium, the supply situation of improvement earth potassium element, increases the absorption of crop to potassium.After the element chemical combination such as the required nitrogen of it and plant, phosphorus, can become multi-effect compound fertilizer material, and can make earth modifying agent, plant growth stimulating and fertilizer efficiency promoting agent, and crop alimentary spray liquid.), (oxyenadenine, alkene acyl adenine are a kind of new and effective plant growth regulator to growth-promoting agent.Can cause cell division, the growth of the formation of induced bud and promotion bud, has the cell of promotion to expand, improve fruit-setting rate, the effect of Delaying Leaf-Senescence can also promote crop to take root and also can delay the degradation speed of chlorophyll and protein under low concentration, prevent Senescence in Detached Leaves, protect green.), protectant (potassium alginate content: >=25%, contain macroelement, in, trace element, alginic acid, the basic element of cell division, auxin precursor, enzyme, hydrolyzed protein, betain; Function: the photosynthesis that strengthens fruit tree; Promote root cell division, stimulate growth and development of fruit tree; Promote fruit growth, maturation; Strengthen disease-resistant, the drought resisting of plant self, the anti-adversity ability such as cold-resistant, disease-resistant yield-increasing successful) and pH value conditioning agent (citric acid, sodium bicarbonate) be mixed with the bacteria agent that is exclusively used in cotton growth promotion, premature senescence resistance, anti-bud drop.
The prepared bacteria agent of the present invention, the secondary substance of generation can promote cotton growth, solves the poky problem of cotton seedling in early stage; can resist cotton premature senescence again, improve the degeneration-resistant and disease resistance of cotton, can also protect flower and protect cotton buds and bolls; coming off of prevention cotton cotton buds and bolls, significantly improves the output of cotton.
Bacteria agent of the present invention just can be executed by continuous spraying (dripping) after emerging from cotton, every 7-10 days, uses once, uses continuously 5 above effects remarkable.That bacteria agent of the present invention is used is simple, convenient, with low cost, safety and environmental protection, cost performance are high.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Strains A CCC30352 is Fusorium moniliforme Sheldon
fusarium monilifformesheldon; Strains A CCC30334 is Fusorium moniliforme Sheldon
fusarium monilifformesheldon;
Strains A CCC31052 is Gibberella zeae
gibberella zeae(Schw.) Petch;
Above-mentioned 3 kinds of bacterial strains are all purchased from Chinese agriculture microorganism fungus kind preservation administrative center;
Liquid fermentation medium formula: mass percent 20% potato juice-1000ml, glucose-20g, KH
2pO
4-3g, MgSO
47H
2o-1.5g, thiamine-0.05g, oxyenadenine-0.3g, isopentennyladenine-0.3g, xanthans-3g, agar-5g, mixes above-mentioned each component, and adjust pH to 6.0, obtains liquid fermentation medium.
embodiment 1
The preparation method of bacteria agent of the present invention is as follows:
One, the zymotic fluid of preparing three kinds of bacterial strains
(1) prepare the zymotic fluid of strains A CCC30352: the liquid fermentation medium for preparing the required quality of fermentation, strains A CCC30352 is accessed in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, sterilizing is equipped with in access, and (autoclaving, sterilising temp is 121 ℃ ~ 125 ℃, pressure 0.103Mpa ~ 0.168Mpa, sterilization time 0.5h ~ 1.0h.) and the fermentation tank of cooled liquid fermentation medium in carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput starts the (v/v.m at 1:1.2, represent per minute interior by the volume of air ratio of unit volume culture fluid), cultivate and after 24 hours, increase to 1:1.5 (v/v.m), after 48 hours, be down to 1:1 (v/v.m), fermentation is complete to fermentation in 72 hours, when in process microscopy zymotic fluid, the bacterium amount of ACCC30352 reaches hundred million/ml of 20-30, is qualified bacterium liquid.
(2) prepare the zymotic fluid of strains A CCC30334: the liquid fermentation medium for preparing the required quality of fermentation, then strains A CCC30334 is accessed in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, sterilizing is equipped with in access, and (autoclaving, sterilising temp is 121 ℃ ~ 125 ℃, pressure 0.103Mpa ~ 0.168Mpa, sterilization time 0.5h ~ 1.0h.) and the fermentation tank of cooled liquid fermentation medium in carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput start 1: 1 (v. v. m), cultivate after 24 hours, increase to 1:1.2 (v. v. m), after 48 hours, continue to increase to 1: 1.5 (v. v. m); Fermentation to 72 hour fermentation is complete, when in process microscopy zymotic fluid, the bacterium amount of ACCC30334 reaches hundred million/ml of 20-30, is qualified bacterium liquid.(3) prepare the zymotic fluid of strains A CCC31052: this preparation method is identical with the preparation method of the zymotic fluid of strains A CCC30334 in step (2).
Two, prepare bacteria agent
By the zymotic fluid 200g of the strains A CCC30352 of above-mentioned preparation, the zymotic fluid 200g of strains A CCC30334, the zymotic fluid 200g of strains A CCC31052, with oxyenadenine 3g, isopentennyladenine 3g, humic acid potassium 100g, potassium alginate 100g, with a small amount of citric acid or sodium bicarbonate, pH value is adjusted to 6.0, surplus is uniformly dissolved with clear water polishing to mix and blend after 1000g, makes bacteria agent of the present invention.
embodiment 2 antagonistic effects
Strains A CCC30352, ACCC30334, ACCC31052, on PDA medium, are placed to mutual spacing 3cm at 3, and each combines in triplicate, intersects antagonistic effect, and result shows that three does not exist antagonism each other, can grow jointly.
embodiment 3 measures the biologically active of bacteria agent of the present invention
Apply respectively microbial inoculum ACCC30352, ACCC30334, ACCC31052 and bacteria agent of the present invention and carry out biologically active comparison, and contrast with clear water, carry out the test of growth promotion, premature senescence resistance, slip-off preventing three aspects:.Wherein growth promotion experiment: above-mentioned microbial inoculum and clear water compare the increase ratio of cotton seedling plant height; Premature senescence resistance experiment: the incidence of more above-mentioned microbial inoculum early ageing cotton plant; Slip-off preventing experiment: the ratio of more above-mentioned microbial inoculum fruit abscission rate, result is as shown in table 1.
Table 1
From the every data of table 1, can find out, no matter bacteria agent of the present invention is at growth promotion, premature senescence resistance, or in the experiment of slip-off preventing, the biologically active of bacteria agent of the present invention all will significantly be better than the biologically active of single creature microbial inoculum.
In actual production, by above-mentioned, make after 1000 times of bacteria agent dilutions, adopt the way of drip irrigation to apply cotton root, from seedling stage to the cotton growth later stage, every about 7-10 days, use once, can effectively promote cotton growth, improve that Resistance Strain of Cotton is contrary, disease-resistant, the ability of premature senescence resistance, the bud drop shedding of cotton bolls phenomenon of effectively preventing cotton.Whole process of growth in cotton does not need to spray gibberellin again.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. for a bacteria agent for cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls, it is characterized in that: described bacteria agent comprises following material: strains A CCC30352, strains A CCC30334 and strains A CCC31052.
2. bacteria agent according to claim 1, is characterized in that: in described bacteria agent, the mass ratio of strains A CCC30352, strains A CCC30334 and strains A CCC31052 is 1:1:1.
3. bacteria agent according to claim 1 and 2, it is characterized in that: described bacteria agent is comprised of each component of following quality percentage composition: the zymotic fluid 20% of strains A CCC30352, the zymotic fluid 20% of strains A CCC30334, the zymotic fluid 20% of strains A CCC31052, oxyenadenine 0.3%, isopentennyladenine 0.3%, humic acid potassium or nitro khm 10%, potassium alginate 10%, pH value is adjusted to 6.0, and surplus is water.
4. the preparation method of the arbitrary described bacteria agent of claim 1-3, is that each component of formula ratio is mixed, and adjust pH to 6.0 obtains.
5. preparation method according to claim 4, it is characterized in that: the preparation method of the zymotic fluid of described strains A CCC30352 is: strains A CCC30352 is accessed in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, access in the fermentation tank that sterilizing cooled liquid fermentation medium are housed and carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput starts at 1:1.2, cultivate and increase to 1:1.5 after 24 hours, after 48 hours, be down to 1:1, fermentation is complete to fermentation in 72 hours, when the bacterium amount of ACCC30352 reaches hundred million/ml of 20-30 in process microscopy zymotic fluid, obtain zymotic fluid.
6. preparation method according to claim 4, it is characterized in that: the preparation method of the zymotic fluid of described strains A CCC30334 and ACCC31052 is: bacterial strain is accessed respectively in liquid fermentation medium under aseptic condition, under 25 ℃ of conditions on shaking table shaken cultivation after 72 hours, all reach 2.0 * 10 to living bacteria count
9individual/ml makes first order seed; Then by cultured first order seed 3% ratio in mass ratio, access in the fermentation tank that sterilizing cooled liquid fermentation medium are housed and carry out fermenting and producing, 25 ℃ of fermented and cultured temperature, throughput starts at 1:1, cultivate and increase to 1:1.2 after 24 hours, after 48 hours, continue to increase to 1:1.5; Fermentation to 72 hour fermentation is complete, when bacterium amount reaches hundred million/ml of 20-30 in process microscopy zymotic fluid, obtains zymotic fluid.
7. according to the preparation method described in claim 5 or 6, it is characterized in that: the preparation method of described liquid fermentation medium is: mass percent 20% potato juice-1000ml, glucose-20g, KH
2pO
4-3g, MgSO
47H
2o-1.5g, thiamine-0.05g, oxyenadenine-0.3g, isopentennyladenine-0.3g, xanthans-3g, agar-5g, mixes above-mentioned each component, and pH value is adjusted to 6.0.
8. the application of the arbitrary described bacteria agent of claim 1-3 in cotton growth promotion, premature senescence resistance, the anti-shedding of cotton bolls.
9. application according to claim 8, is characterized in that: described application is to adopt the method for drip irrigation that bacteria agent is applied to cotton root, from seedling stage to the cotton growth later stage, every about 7-10 days, uses once.
10. application according to claim 9, is characterized in that: the whole process of growth in cotton does not need to spray gibberellin again.
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| CN201310515077.8A CN103609595B (en) | 2013-10-28 | 2013-10-28 | A kind of for Cotton Gossypii growth promotion, premature senescence resistance, the bacteria agent and its preparation method and application of the anti-shedding of cotton bolls |
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| CN201310515077.8A CN103609595B (en) | 2013-10-28 | 2013-10-28 | A kind of for Cotton Gossypii growth promotion, premature senescence resistance, the bacteria agent and its preparation method and application of the anti-shedding of cotton bolls |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
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| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
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