CN103609225A - Preservation method for plant orthodox seeds - Google Patents
Preservation method for plant orthodox seeds Download PDFInfo
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- CN103609225A CN103609225A CN201310573409.8A CN201310573409A CN103609225A CN 103609225 A CN103609225 A CN 103609225A CN 201310573409 A CN201310573409 A CN 201310573409A CN 103609225 A CN103609225 A CN 103609225A
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Abstract
The invention discloses a preservation method for plant orthodox seeds and belongs to the technical field of biology. The method comprises the following steps: washing seeds; removing impurities and rinsing cleanly; then naturally drying the seeds on a shady and ventilated place; drying the seeds under a freezing vacuum condition; filling the dried seeds into an ampoule pipe and plugging a cotton plug; and vacuumizing the ampoule pipe and hotly sealing. According to the preservation method, the seeds are preserved by the ampoule so that the service life of the seeds is prolonged effectively and the storage life of the seeds is prolonged; the method is low in cost, low in cost and small in occupying space; the seeds are long in storage time and are easy to manage and transport.
Description
Technical field
The present invention relates to biological technical field, particularly a Plants normality seed store method.
Background technology
Preservation of plant germplasin is conducive to protective plant resource diversity and genetic diversity.According to different objects, the method for Preservation of plant germplasin is broadly divided into original place preservation, preserve in strange land and facility is preserved three kinds.Seed is the main body of plant germplasm resource, sets up to take the most reliable easily method of row that Preservation of plant germplasin that seed germplasm is main body is acknowledged as conservation of plant resources of collecting.At present, the store method of seed mainly contains dry Tibetan and wets and hide two kinds.Before the dry seed warehouse-in of hiding, must measure water content and disinfect, the seed high to water content will be dried, and the seed demand of long-term storage in bulk is set up low temperature storehouse.Yet the construction investment in low temperature storehouse is larger, specification requirement is high, and running cost is expensive throughout the year.Wet Tibetan is applicable to the seed that safe water content is higher more.Existing store method takes up room greatly mostly, high to seed moisture content and environmental temperature requirement, when seed moisture content is too high, physiological activity strengthens, and discharges mixing steam, produces amount of heat simultaneously, make seed easily go mouldy and rot, cause seed quality to decline; When water content is too low, seed longevity is exerted an influence; In storage environment, during excess Temperature, seed enzymic activity strengthens, and has strengthened equally seed physiology movable, reduces seed quality; When reserve temperature is too low, high to equipment requirement, management is restricted, and the cost of storage increases.In addition, due to the impact of microorganism and plague of rats insect pest, seed easily goes mouldy and pollutes, and storage effect is not good enough.
Seed is in storage, and seed moisture content and storage environment temperature are the Main Factors that affects the seed storage life-span.Aspect the preservation of matrimony vine germ plasm resource, store method expense is high at present, takes up room large, is difficult to management and transportation.
Summary of the invention
In order to solve prior art, to preserve the expense of seed high, take up room large, and the problem that is difficult to management and transportation, the embodiment of the present invention provides a Plants normality seed store method.Described technical scheme is as follows:
Wash out seed, after removal of impurities rinsing are clean, be placed in the natural seasoning of shady and cool ventilation place;
Described seed is dried under freezing vacuum condition;
Pack described dried seed into ampulla pipe, and jam-pack tampon;
By the vacuum pumping of described ampulla pipe sealing by fusing.
The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is:
The present invention preserves seed by ampulla, effectively extends seed longevity, improves the storage life of seed, and the method cost is low, and expense is few, take up room little, storage time is long, is easy to management and transportation, is having its unique advantage and use value aspect germ plasm resource preservation.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, embodiment of the present invention is described further in detail.
Fig. 1 shows the realization flow of a Plants normality seed store method of embodiment of the present invention proposition, and details are as follows:
In step S101, wash out seed, after removal of impurities rinsing are clean, be placed in the natural seasoning of shady and cool ventilation place;
In an embodiment of the present invention, preferred, only take and from Chinese wolfberry fruit, wash out seed and describe as example.
As a preferred embodiment of the present invention, washing out seed, after removal of impurities rinsing are clean, after being placed in the natural seasoning of shady and cool ventilation place, also comprise:
In described seed, add the skim milk through sterilizing.By skim milk sterilizing 30min under 0.05Mpa condition, for preventing from destroying its nutrient component, do not do heat treated.
In step S102, seed is predrying under liquid nitrogen or-80 ℃ of condition of ultralow temperature;
Ultralow temperature is that structure, the structural material of protection seed is not destroyed.
In step S103, seed is dried under freezing vacuum condition;
In an embodiment of the present invention, profit son being carried out under freezing vacuum condition to dry method has and comprises: the seed packet that matrimony vine is equipped with to matrimony vine seed immerses that in liquid nitrogen, to reach snap frozen dry; Be placed on-80 ℃ of freezing 4～5hr of ultra low temperature freezer.Through the dry seed of processing of above-mentioned two kinds of snap frozen, further pass through again drying under reduced pressure.Its principle is first that matrimony vine seed is freezing, then under reduced pressure, utilizes distillation phenomenon to remove moisture, makes seed moisture content content below 4%.In fact, remove after most of moisture from seed, the physiological activity of cell will weaken, and therefore can reach the object of long term maintenance life state.
In step S104, pack dried seed into ampulla pipe, and jam-pack tampon;
In an embodiment of the present invention, ampulla pipe and tampon are through autoclaving.Ampulla pipe and tampon are put into pressure cooker sterilizing 30min.
In step S105. by the vacuum pumping of ampulla pipe sealing by fusing;
In an embodiment of the present invention, the step of the vacuum pumping of ampulla pipe sealing by fusing is specifically comprised:
By filling seed-bearing ampulla pipe, put into vacuum cooling drying bottle, by cooling water, make seed be frozen into solid;
By extracting vacuum freeze drying bottle air, make water sublimed, be then dried;
Take out ampulla pipe, and it is connected with vavuum pump, after vacuumizing, by ampulla pipe sealing by fusing.
Ampulla is put into the bell jar of vacuum freeze dryer, started shooting and open after cooling water, make seed quick freezing become solid, then vacuumize and make water sublimed, after 2 hours, can stop freezing and be dried at slightly high temperature, until absolutely dry.Finally take out ampulla pipe, and it is connected with vavuum pump, then vacuumize after (pressure-0.08Mpa), rapidly by ampulla pipe sealing by fusing.
In an embodiment of the present invention, the temperature of ampulla pipe sealing by fusing is 1500 ℃.
In step S106. the ampulla pipe after sealing by fusing is placed under normal temperature or low temperature to be preserved, and such as family expenses general refrigerator refrigerating chamber, temperature is generally 4 ℃ of left and right.
The embodiment of the present invention is preserved seed by ampulla, effectively extends seed longevity, improves the storage life of seed, and the method cost is low, and expense is few, take up room little, storage time is long, is easy to management and transportation, is having its unique advantage and use value aspect germ plasm resource preservation.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (6)
1. a Plants normality seed store method, is characterized in that, described method comprises:
Wash out seed, after removal of impurities rinsing are clean, be placed in the natural seasoning of shady and cool ventilation place;
Described seed is dried under freezing vacuum condition;
Pack described dried seed into ampulla pipe, and jam-pack tampon;
By the vacuum pumping of described ampulla pipe sealing by fusing.
2. method according to claim 1, is characterized in that, described in wash out seed, after removal of impurities rinsing are clean, also comprise after being placed in the natural seasoning of shady and cool ventilation place:
In described seed, add the skim milk through sterilizing.
3. method according to claim 1, is characterized in that, described ampulla pipe and tampon are through autoclaving.
4. method according to claim 1, is characterized in that, the described method by the vacuum pumping of described ampulla pipe sealing by fusing specifically comprises the steps:
By described, fill seed-bearing ampulla pipe and put into vacuum cooling drying bottle, by cooling water, make seed be frozen into solid;
By extracting described vacuum cooling drying bottle air, make water sublimed, be then dried;
Take out described ampulla pipe, and it is connected with vavuum pump, after vacuumizing, by described ampulla pipe sealing by fusing.
5. method according to claim 4, is characterized in that, the ampulla pipe after described sealing by fusing is placed under normal temperature or low temperature to be preserved.
6. method according to claim 1 or 5, is characterized in that, the temperature of described ampulla pipe sealing by fusing is 1500 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573409.8A CN103609225B (en) | 2013-11-14 | 2013-11-14 | Preservation method for plant orthodox seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573409.8A CN103609225B (en) | 2013-11-14 | 2013-11-14 | Preservation method for plant orthodox seeds |
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| Publication Number | Publication Date |
|---|---|
| CN103609225A true CN103609225A (en) | 2014-03-05 |
| CN103609225B CN103609225B (en) | 2015-03-25 |
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| CN201310573409.8A Expired - Fee Related CN103609225B (en) | 2013-11-14 | 2013-11-14 | Preservation method for plant orthodox seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
| CN112930747A (en) * | 2021-01-20 | 2021-06-11 | 苏州三亩良铺农业科技有限公司 | Crop seed micro-freezing method and application thereof |
| CN113875749A (en) * | 2021-11-11 | 2022-01-04 | 塔里木大学 | Ultralow temperature preservation method for tamarix seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039601A (en) * | 2013-01-16 | 2013-04-17 | 安徽省农业科学院水稻研究所 | Method for improving storage tolerance of aged seeds of plant |
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2013
- 2013-11-14 CN CN201310573409.8A patent/CN103609225B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039601A (en) * | 2013-01-16 | 2013-04-17 | 安徽省农业科学院水稻研究所 | Method for improving storage tolerance of aged seeds of plant |
Non-Patent Citations (1)
| Title |
|---|
| 胡晋等: "《种子贮藏加工学》", 31 December 2010 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
| CN112514751B (en) * | 2020-11-30 | 2022-06-17 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
| CN112930747A (en) * | 2021-01-20 | 2021-06-11 | 苏州三亩良铺农业科技有限公司 | Crop seed micro-freezing method and application thereof |
| CN112930747B (en) * | 2021-01-20 | 2022-03-11 | 苏州三亩良铺农业科技有限公司 | Crop seed micro-freezing method and application thereof |
| CN113875749A (en) * | 2021-11-11 | 2022-01-04 | 塔里木大学 | Ultralow temperature preservation method for tamarix seeds |
| CN113875749B (en) * | 2021-11-11 | 2022-07-15 | 塔里木大学 | Ultralow temperature preservation method for tamarix seeds |
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| CN103609225B (en) | 2015-03-25 |
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Granted publication date: 20150325 Termination date: 20161114 |