CN109717354A - A kind of method of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis - Google Patents
A kind of method of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis Download PDFInfo
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- CN109717354A CN109717354A CN201910175562.2A CN201910175562A CN109717354A CN 109717354 A CN109717354 A CN 109717354A CN 201910175562 A CN201910175562 A CN 201910175562A CN 109717354 A CN109717354 A CN 109717354A
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- cordyceps sinensis
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Abstract
The present invention relates to a kind of methods of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis, belong to cordyceps sinensis technical field of preservation of fresh.Method provided by the invention obtains the cordyceps sinensis of package soil the following steps are included: the cordyceps sinensis of fresh acquisition 1) is superscribed soil；2) cordyceps sinensis for the package soil that step 1) obtains is placed in cool-bag or immersion liquid nitrogen flash freezer device, liquid nitrogen, quick-frozen 5~30min is added；3) cordyceps sinensis of the step 2) after quick-frozen is placed in -18~-86 DEG C of refrigerators to save, or continues to be placed in liquid nitrogen and saves.Method provided by the invention is easy to operate, equipment degree of dependence is low, without ancillary additions matter, it is fresh cordyceps sinensis source area preservation method that can be fresh-keeping for a long time, for the high-value-use and new-product development of valuable medicinal cordyceps sinensis fresh products, provides the support of raw material preservation technology.
Description
Technical field
The present invention relates to cordyceps sinensis technical field of preservation of fresh, and in particular to a kind of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis
Method.
Background technique
The growth of natural cordyceps is extremely harsh to ecological geographical conditions, can only high and cold grass more than height above sea level 3000m
The growth of pasture area.Due to lacking effective fresh cordyceps sinensis place of production preservation technology, limited by Habitat producing condition, in order to reduce
Loss of the fresh cordyceps sinensis in the transportational process from the place of production to factory, fresh cordyceps sinensis are at source processed in a manner of natural drying etc.
At dry cordyceps sinensis, then transports to factory and carry out advanced treating.
The missing of place of production preservation method technology, causes:
1, product form is single: cordyceps sinensis product is based on dry products, lack of diversity, is not able to satisfy people to the winter
The demand of worm summer grass product form.
2, effective component is destroyed, pharmacological activity decline: pharmaceutical research shows compared with dry cordyceps sinensis, fresh
Cordyceps sinensis all has better effects in terms of the pharmacological actions such as immunological regulation, antitumor, anti-oxidant.
3, transport loss is excessively high: the limitation of Habitat producing condition, the dry processing of primary, dry product transport, secondary operation etc.
Process leads to " disconnected grass ", and rate increases, and the transport of fresh cordyceps sinensis will lead to and rot.
Currently, being concentrated mainly on preservation by low temperature, quick-freezing fresh-keeping, controlled atmosphere for the research of cordyceps sinensis store and processing
Deng.The principle of cordyceps sinensis preservation by low temperature is mainly the respiration for inhibiting living cells, the metabolism for reducing cell, and low temperature is protected
The temperature for depositing cordyceps sinensis is normally controlled in -3~-10 DEG C.The temperature of quick-freezing fresh-keeping is normally controlled in -80 DEG C.Controlled atmosphere
Principle is to obtain the regulating gas different from normal atmosphere component, suppression by various regulative modes in certain closed system
System leads to the activity of corrupt physiological and biochemical procedure and microorganism.In addition, deepening continuously with the fresh-keeping research of fresh cordyceps sinensis, mesh
The generation of the preceding intelligent fresh-keeping system for having had been reported that fresh cordyceps sinensis is simultaneously applied in practice, mainly passes through intelligent program
Regulate and control cold storing and fresh-keeping temperature, time, the parameters such as humidity, from cell level keep fresh cordyceps sinensis fresh vitality and nutrition at
Point.But above-mentioned preservation technology is higher to the dependence of freshness preserving equipment, operation is relatively complicated, and it is fresh-keeping and new to be unsuitable for source area
Fresh cordyceps sinensis cannot save for a long time.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis.It is provided by the invention
Method is easy to operate, equipment degree of dependence is low, no ancillary additions matter, is that fresh cordyceps sinensis that can be fresh-keeping for a long time originates in
Ground preservation method provides raw material fresh-keeping skill the high-value-use and new-product development of valuable medicinal cordyceps sinensis fresh products
Art is supported.
The present invention provides a kind of methods of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis, comprising the following steps:
1) cordyceps sinensis of fresh acquisition is superscribed into soil, obtains the cordyceps sinensis of package soil；
2) cordyceps sinensis for the package soil that step 1) obtains is placed in cool-bag or immersion liquid nitrogen flash freezer device
In, liquid nitrogen, quick-frozen 5~30min is added；
3) cordyceps sinensis of the step 2) after quick-frozen is placed in -18~-86 DEG C of refrigerators to save, or continues to be placed in liquid nitrogen and protects
It deposits.
Preferably, the step 1) soil includes the soil of cordyceps sinensis locality.
Preferably, soil package with a thickness of 1~10mm.
Preferably, the step 2) cool-bag includes thermos flask, vacuum cup, insulation barrel, foam insulation box, Dewar bottle
Or liquid nitrogen container.
Preferably, step 2) the immersion liquid nitrogen flash freezer device includes liquid nitrogen deep cryogenic box.
Preferably, the cordyceps sinensis of step 2) the package soil and the mixing mass ratio of liquid nitrogen are 1:(2~20).
Preferably, the quick-frozen operating time is collected no more than 12h from fresh.
The present invention provides a kind of methods of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis.The present invention is former for cordyceps sinensis
Natural conditions and facility, the fresh-keeping demand of appointed condition and fresh cordyceps sinensis in the place of production, obtained method is easy to operate, equipment
Degree of dependence is low, no ancillary additions matter, is a kind of fresh cordyceps sinensis source area preservation method that can be fresh-keeping for a long time, right
In the high-value-use and new-product development of valuable medicinal cordyceps sinensis fresh products, the support of raw material preservation technology is provided.
Method provided by the invention has the advantage that
(1) chilling rate is fast (freezing speed is 30~40 times about faster than general freezing method): using liquid nitrogen flash freezer, can make to eat
Product run through 0 DEG C~5 DEG C maximum ice-crystal growth bands.
(2) without ancillary additions matter, holding food quality: this method does not add the ancillary additions matter such as antistaling agent, and
Since the time of liquid nitrogen flash freezer is short, the cordyceps sinensis through liquid nitrogen flash freezer can keep to the maximum extent color under fresh state, perfume (or spice),
Taste and nutritive value.
(3) material drying loss is small: the drying loss loss late generally freezed is 3~6%, and liquid nitrogen flash freezer may decrease to 0.25~
0.5%.
(4) easy to operate, equipment degree of dependence is low: the prior art is more demanding to control of temperature and humidity, and fresh-keeping freezing
Equipment needs continued power, is unsuitable for cordyceps sinensis source area facility, appointed condition, and this method is not necessarily to rely on equipment, and is not required to
Continued power, cool-bag of the invention or immersion liquid nitrogen flash freezer device are wanted, can be used for unregulated power environment.
(5) fresh keeping time is long: compared with the conventional method, the method for the invention is more suitable for the long-term guarantor of fresh cordyceps sinensis
Fresh, especially fresh keeping time is longer, and the method for the invention advantage is more obvious.
Specific embodiment
The present invention provides a kind of methods of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis, comprising the following steps:
1) cordyceps sinensis of fresh acquisition is superscribed into soil, obtains the cordyceps sinensis of package soil；
2) cordyceps sinensis for the package soil that step 1) obtains is placed in cool-bag or immersion liquid nitrogen flash freezer device
In, liquid nitrogen, quick-frozen 5~30min is added；
3) cordyceps sinensis of the step 2) after quick-frozen is placed in -18~-86 DEG C of refrigerators to save, or continues to be placed in liquid nitrogen and protects
It deposits.
The cordyceps sinensis of fresh acquisition is superscribed soil by the present invention, obtains the cordyceps sinensis of package soil.In the present invention
In, the soil includes the soil of cordyceps sinensis locality.In the present invention, the thickness of soil package be preferably 1~
10mm, more preferably 3~5mm, most preferably 4mm.Soil is preferably equably wrapped in the worm summer in winter of fresh acquisition by the present invention
On grass.
After obtaining the cordyceps sinensis of package soil, the cordyceps sinensis for wrapping up soil is placed in cool-bag or sunk by the present invention
In immersion liquid nitrogen flash freezer device, liquid nitrogen, quick-frozen 5~30min is added.In the present invention, the quick-frozen time be preferably 5~
15min, more preferably 10min.In the present invention, the cool-bag preferably includes thermos flask, vacuum cup, insulation barrel, foam
Incubator, Dewar bottle or liquid nitrogen container.In the present invention, the immersion liquid nitrogen flash freezer device preferably includes liquid nitrogen deep low temperature
Case.The present invention does not have special restriction to the source of the liquid nitrogen deep cryogenic box, using conventional commercial product, such as Xi'an
The liquid nitrogen deep cryogenic box of Heng Mao power Science and Technology Ltd. production.In the present invention, it is described package soil cordyceps sinensis with
The mixing mass ratio of liquid nitrogen is 1:(2~20), more preferably 1:5~10, most preferably 1:8.
In the present invention, the quick-frozen operating time is collected no more than 12h, more preferably no more than 2h from fresh.
After quick-frozen, the present invention will be quick-frozen after cordyceps sinensis be placed in -18~-86 DEG C of refrigerators and save, or continue to be placed in liquid nitrogen
Middle preservation.
Combined with specific embodiments below to a kind of method of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis of the present invention
It is further described in detail, technical solution of the present invention includes but is not limited to following embodiment.
Embodiment 1
By the cordyceps sinensis 0.5kg of fresh acquisition, locality soil, soil thickness 10mm are uniformly wrapped up.Soil will be wrapped up
Fresh cordyceps sinensis be placed in foam insulation box with cover, be added 10kg liquid nitrogen, quick-frozen 30min.Cordyceps sinensis after quick-frozen
It is put into -18 DEG C of low temperature refrigerator preservations.
Embodiment 2
By the cordyceps sinensis 0.12kg of fresh acquisition, locality soil, soil thickness 1mm are uniformly wrapped up.Soil will be wrapped up
Fresh cordyceps sinensis be placed in vacuum thermosbottle, be added 0.6kg liquid nitrogen, quick-frozen 5min.Cordyceps sinensis after quick-frozen is put into
- 40 DEG C of ultra low temperature freezer preservations.
Embodiment 3
By the cordyceps sinensis 1kg of fresh acquisition, locality soil, soil thickness 5mm are uniformly wrapped up.The new of soil will be wrapped up
Fresh cordyceps sinensis is placed in liquid nitrogen container, and 2kg liquid nitrogen, quick-frozen 5min is added.Cordyceps sinensis continuation after quick-frozen is protected in liquid nitrogen
It deposits.
Embodiment 4
By the cordyceps sinensis 0.3kg of fresh acquisition, locality soil, soil thickness 7mm are uniformly wrapped up.Soil will be wrapped up
Fresh cordyceps sinensis is placed in insulation barrel, and 3kg liquid nitrogen, quick-frozen 15min is added.Cordyceps sinensis after quick-frozen is put into ultralow temperature ice
- 86 DEG C of case preservations.
Embodiment 5
By the cordyceps sinensis 0.8kg of fresh acquisition, locality soil, soil thickness 8mm are uniformly wrapped up.Soil will be wrapped up
Fresh cordyceps sinensis is placed in foam insulation box, and 7.5kg liquid nitrogen, quick-frozen 20min is added.Cordyceps sinensis after quick-frozen is put into low
- 20 DEG C of temperature refrigerator preservations.
Reference examples 1
Chinese patent (CN 106720232A) discloses a kind " storing and fresh-keeping method of fresh cordyceps sinensis ", which uses
The methods of Low-temperature cleaning, low temperature classification, classification preservation, entire technique carries out at low ambient temperatures, makes the fresh-keeping of fresh cordyceps sinensis
Time greatly prolongs.In 5~10 DEG C of washings, classification, -3~-10 DEG C, save under the conditions of humidity 60~75%, fresh cordyceps sinensis
Fresh keeping time can extend to 75 days.
Chinese patent (CN 102178226A) discloses " a kind of fresh processing technique of cordyceps sinensis ", which, which includes, adopts
With ultrasonic cleaning, sterilizing is sealed, and fresh-keeping in ultralow temperature refrigerating equipment, the methods of storage, fresh cordyceps sinensis is under zero degree
Preservation by low temperature state prevents the heat-sensitive substance contained in cordyceps sinensis from denaturation or devitalized occurs.
Chinese patent (CN 103740592A) discloses " a kind of preservation method of cordyceps sinensis ", which includes using stream
Water cleaning, 0.9% acidic sodium chloride solution impregnate, quickly cooling under conditions of -75 DEG C, controlled atmosphere cold storage at a temperature of -5 DEG C~-25 DEG C, makes
Obtain the nutritional ingredient of cordyceps sinensis and the basic free of losses of activity after saving for a long time.
Chinese patent (CN 108835502A) discloses a kind " fresh cordyceps sinensis product and preparation method thereof ", which includes
Using the methods of soaking in Mel, smearing, fresh cordyceps sinensis fresh keeping product is prepared, fresh-keeping effect is preferable under the conditions of room temperature, energy
Enough utmostly retain the original nutritional ingredient of fresh cordyceps sinensis and form, long shelf-life, mouthfeel are good.
Compared with above-mentioned 4 patented methods, the method for the present invention at fresh-keeping to the 75th day, fresh cordyceps sinensis weight-loss ratio,
Soluble protein content, polyphenol oxidase activity etc. are superior to the fresh-keeping cordyceps sinensis of other methods.Comparative experiments and result
It is as follows:
Fresh cordyceps sinensis 270 of 0.5~1.0g specification are taken, 9 groups are randomly divided into, respectively according to patent CN
The method processing and sheet of 106720232A, patent CN 102178226A, patent CN 103740592A, patent CN108835502A
The method of inventive embodiments 1~5 is handled, and each group name that handles is respectively preservation by low temperature group 1~4 and fresh-keeping group 1~5 of liquid nitrogen.It surveys
When being scheduled on preservation the 75th day, each group cordyceps sinensis sample is relative to the weight-loss ratio before Preservation Treatment, soluble protein content decline
Rate, polyphenol oxidase activity growth rate.
Sample weight loss rate is according to " Wang Jingbo waits influence [J] of .3 kind plants essential oil suffocating treatment to nectarine fresh-keeping effect
Nuclear agricultural science report, 2018,32 (5): the measurement of 933-940. " method, soluble protein content rate of descent is according to " Deng Lili waits to examine horse
This bright trip's method measurement condition optimizing [J] the Food Science of apple tissue trace soluble egg from content, 2012, (24): 185-
The measurement of 189. " methods, polyphenol oxidase activity growth rate is according to " north postharvest fruit and vegetable bio-chemical characteristics guidance [M] is waited in Jiankang
Capital: China Light Industry Press, the measurement of 2007:59-154. " method, the results are shown in Table 1.When saving the 75th day, the present invention
The fresh-keeping cordyceps sinensis of method, in terms of weight-loss ratio, soluble protein content rate of descent and polyphenol oxidase activity growth rate,
It is fresh-keeping better than Chinese patent CN106720232A, CN 102178226A, CN 103740592A and CN 108835502A method
Cordyceps sinensis.Meanwhile the fresh-keeping effect of the method for the invention is more obvious with the extension advantage of fresh keeping time.
Table 1 saves the 75th day, the weight-loss ratio of each processing group cordyceps sinensis sample, soluble protein content decline
Rate and polyphenol oxidase activity growth rate measurement result
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of method of liquid nitrogen immersion quick-freezing fresh-keeping cordyceps sinensis, comprising the following steps:
1) cordyceps sinensis of fresh acquisition is superscribed into soil, obtains the cordyceps sinensis of package soil；
2) cordyceps sinensis for the package soil that step 1) obtains is placed in cool-bag or immersion liquid nitrogen flash freezer device, is added
Enter liquid nitrogen, quick-frozen 5~30min；
3) cordyceps sinensis of the step 2) after quick-frozen is placed in -18~-86 DEG C of refrigerators to save, or continues to be placed in liquid nitrogen and saves.
2. the method according to claim 1, wherein the step 1) soil includes the mud of cordyceps sinensis locality
Soil.
3. method according to claim 1 or 2, which is characterized in that soil package with a thickness of 1~10mm.
4. the method according to claim 1, wherein the step 2) cool-bag include thermos flask, vacuum cup,
Insulation barrel, foam insulation box, Dewar bottle or liquid nitrogen container.
5. the method according to claim 1, wherein step 2) the immersion liquid nitrogen flash freezer device includes liquid nitrogen
Deep cooling cryogenic box.
6. the method according to claim 1, wherein the cordyceps sinensis and liquid nitrogen of step 2) the package soil
Mixing mass ratio is 1:(2~20).
7. the method according to claim 1, wherein collecting the quick-frozen operating time no more than 12h from fresh.
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Cited By (1)
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| CN111990578A (en) * | 2020-08-26 | 2020-11-27 | 尹浩 | Fresh freezing storage method of natural cordyceps sinensis |
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