CN103599570B - Injectable fibrin-calcium phosphate composite structure with osteogenesis effect - Google Patents
Injectable fibrin-calcium phosphate composite structure with osteogenesis effect Download PDFInfo
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- CN103599570B CN103599570B CN201310517958.3A CN201310517958A CN103599570B CN 103599570 B CN103599570 B CN 103599570B CN 201310517958 A CN201310517958 A CN 201310517958A CN 103599570 B CN103599570 B CN 103599570B
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- calcium phosphate
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- fibrinogen solution
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Abstract
The invention relates to an injectable fibrin-calcium phosphate composite structure with an osteogenesis effect. The composite structure contains three components, namely fibrous protein raw-powder, a phosphate buffer and a nanometer self-solidifying calcium phosphate powder. When in use, the fibrous protein raw-powder is firstly dissolved in the phosphate buffer to prepare 100mg/ml of a fibrinogen solution; and then, the nanometer self-solidifying calcium phosphate powder and the fibrinogen solution are combined according to the ratio of 1:1 (g/ml) to form the injectable composite structure with an inductive osteogenesis capability. The structure is injectable, and can be percutaneously injected to a bone defect part through an injection needle and solidified within 30 minutes to perform a dual function of filling and supporting. Finally, the composite component can generate an osteogenesis promotion effect on the bone defect part by chemotaxis and spatial effect, and the bone defect can be restored successfully.
Description
Technical field
The invention belongs to field of medicine production, relate to a kind of injectable type composite construction of short ossification, be particularly useful for the invasive treatment of Cranial defect.
Background technology
One for the treatment of difficult problem being still field of orthopaedics of Cranial defect caused by various wound, infection and osteopathia, bone collection is still the best approach by surgical intervention, repairing bone defect.Be regarded as bone collection " goldstandard " although autologous bone transplanting safety, effectively, for the case needing a large amount of bone grafting, limited autologous bone source still can not meet operation needs.Allosome or bone xenograft supplement because the problems such as immunologic rejection, transmitted virus, limited source and ethics custom are difficult to become the strong of bone graft source.Organizational project theory and technology brings new hope for building " safe and effective bone graft substitute ", but present stage exogenous seed cell immunogenicity, the difficult problem such as potential oncogenicity and great number cost limits the extensive use of tissue engineering product.
Composite growth factor (as bone morphogenetic protein, transforming growth factor etc.) is the common experimental method improving embedded material Bone Defect Repari effect.But in clinical practice, the bony defect of required reparation is often larger.Once throw in somatomedin to be difficult to satisfy the demand.And Cranial defect perilesional usually has infection tendency, somatomedin usually needs competence exertion effect after infection control.So, also need to carry out slow release to somatomedin.And body fluid, temperature and pH value etc. all can affect exogenous growth factor long-term preservation in vivo.In addition, somatomedin use cost is high, requires harsh, can not meet the needs of routine clinical treatment to links such as sterilization, transport and storages.Therefore, need to find a kind of stable, effectively facilitate bone method to carry out alternative somatomedin playing a role in implantation local.
Because how irregular Cranial defect shape is, sometimes also there is sidewalk for visually impaired people and dead space.External preforming bone-grafting material is difficult to adapt to Cranial defect shape, and normally certainly will form interval between sclerotin, not only affects the bone conduction effect of material.Owing to being difficult between some defects effectively fill bone-grafting material, also causing subsides around bone grafting affects repairing effect.Therefore, external preforming bone grafting product in use, often needs the tissue around to implant bed appear widely and peel off, and certainly will destroy widely the soft tissue of defect and blood for causing.Soft tissue around the destruction implant bed that research discovery is too much and blood confession, also can significantly reduce Bone Defect Repari effect.
Therefore, development one can be implanted by Wicresoft, and freely fills in Cranial defect, and within a certain period of time according to defect shape curing molding and to produce the bone grafting structure of supporting role significant.
Summary of the invention
The object of the invention is to the shortcoming overcoming above-mentioned prior art, a kind of injectable type fibrin-calcium phosphate composite construction with short ossification is provided, this structure can be injected by transdermal topical Wicresoft, reduce over the destruction that many peeling operations supply soft tissue and blood to produce, and effectively can fill defect, play Bone Defect Repari effect.
The object of the invention is to solve by the following technical programs:
This injectable type fibrin-calcium phosphate composite construction with ossification, containing Fibrinogen powder, phosphate buffer and nanometer self setting calcium phosphate powder three kinds of compositions, first Fibrinogen powder is dissolved in the fibrinogen solution that phosphate buffer is made into 100mg/ml, then, again by nanometer self setting calcium phosphate powder and fibrinogen solution compound, form the fibrin-calcium phosphate composite construction with induced osteogenesis ability; Wherein add the nanometer self setting calcium phosphate powder of 1g in every milliliter of fibrinogen solution.
Fibrin-calcium phosphate composite construction after above-mentioned nanometer self setting calcium phosphate powder and fibrinogen solution compound has syringeability, can by injection needle apparatus percutaneous injection to Cranial defect position.
Behind above-mentioned fibrin-calcium phosphate composite construction injection Cranial defect position, whole defect can be filled up according to defect shape, and solidify in 30 minutes, produce the dual function of filling and supporting.
Injectable type fibrin-calcium phosphate the composite construction with ossification of the present invention according to the actual needs of defect size, effectively can fill defect, plays Bone Defect Repari effect.
Meanwhile, because composite construction of the present invention is in a liquid state in early days in injection, it effectively can be filled according to defect shape, effectively can support again, reach certain mechanical strength after it solidifies completely to sclerotin around defect.Research finds that the complex built according to this component ratio has significant short ossification, and it not only can produce chemotaxis to implanting pre-osteoblast around, and can induce these cell Osteoblast Differentiation, finally plays ossification.Because complex constituent does not relate to somatomedin, avoid many drawbacks.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Wherein: 1 is Fibrinogen powder; 2 is phosphate buffer; 3 is nanometer self setting calcium phosphate powder; 4 is fibrinogen solution.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail:
See Fig. 1, injectable type fibrin-calcium phosphate the composite construction with ossification of the present invention, first Fibrinogen powder (1) is dissolved in the fibrinogen solution (4) that phosphate buffer (2) is made into 100mg/ml, then, again by nanometer self setting calcium phosphate powder (3) and fibrinogen solution (4) according to 1:1(g/ml) ratio is compounded to form a kind of injectable type composite construction with induced osteogenesis ability, namely adds the nanometer self setting calcium phosphate powder (3) of 1g in every milliliter of fibrinogen solution (4).Fibrin of the present invention-this structure of calcium phosphate composite construction has injectable type, by injection needle apparatus percutaneous injection to Cranial defect position, and can solidify in 30 minutes, produces the dual function of filling and supporting.Finally, composite parts can produce short ossification by chemotaxis and steric effect in Cranial defect local, and successful repairing bone defect.
In sum, injectable type fibrin-calcium phosphate the composite construction with ossification that the present invention proposes can meet the needs of bone defect healing in clinical practice, the repairing effect of effective raising Cranial defect, reduces the destruction of routine operation to implant site, significantly saves use cost.
Claims (1)
1. one kind has the preparation method of the injectable type fibrin-calcium phosphate composite construction of ossification, it is characterized in that: by Fibrinogen powder (1), phosphate buffer (2) becomes to be grouped into (3) three kinds, nanometer self setting calcium phosphate powder, first Fibrinogen powder (1) is dissolved in the fibrinogen solution (4) that phosphate buffer (2) is made into 100mg/ml, then, again nanometer self setting calcium phosphate powder (3) is mixed with fibrinogen solution (4), form the fibrin-calcium phosphate composite construction with induced osteogenesis ability, wherein add the nanometer self setting calcium phosphate powder (3) of 1g in every milliliter of fibrinogen solution (4), fibrin-calcium phosphate composite construction after described nanometer self setting calcium phosphate powder (3) and fibrinogen solution (4) compound has syringeability, can by injection needle apparatus percutaneous injection to Cranial defect position, behind described fibrin-calcium phosphate composite construction injection Cranial defect position, whole defect can be filled up according to defect shape, and solidify in 30 minutes, produce the dual function of filling and supporting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310517958.3A CN103599570B (en) | 2013-10-28 | 2013-10-28 | Injectable fibrin-calcium phosphate composite structure with osteogenesis effect |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310517958.3A CN103599570B (en) | 2013-10-28 | 2013-10-28 | Injectable fibrin-calcium phosphate composite structure with osteogenesis effect |
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| Publication Number | Publication Date |
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| CN103599570A CN103599570A (en) | 2014-02-26 |
| CN103599570B true CN103599570B (en) | 2015-04-22 |
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| CN201310517958.3A Expired - Fee Related CN103599570B (en) | 2013-10-28 | 2013-10-28 | Injectable fibrin-calcium phosphate composite structure with osteogenesis effect |
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0166263A1 (en) * | 1984-05-31 | 1986-01-02 | Green Cross Corporation | Filler composition for filling in defect or hollow portion of bone and kit or set for the preparation of the filler composition |
| CN101066473B (en) * | 2007-05-29 | 2010-10-06 | 浙江大学 | Preparation process of fibrin gel-nanometer Ca and P particle composite rack |
| CN102335456A (en) * | 2010-07-21 | 2012-02-01 | 苏州大学附属第一医院 | Calcium phosphate bone cement and preparation method thereof |
| CN102552985B (en) * | 2012-01-20 | 2014-03-19 | 苏州大学 | Silk fibroin/calcium phosphate bone cement-based porous composite material and preparation method thereof |
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