CN109985275A - Shaping DBM self-bone grafting self-curing bone-grafting material and preparation method thereof - Google Patents
Shaping DBM self-bone grafting self-curing bone-grafting material and preparation method thereof Download PDFInfo
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/42—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
- A61L27/425—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of phosphorus containing material, e.g. apatite
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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Abstract
The invention belongs to biomedical materials fields, and in particular to a kind of shaping DBM self-bone grafting self-curing bone-grafting material and preparation method thereof.The bone-grafting material is made of powder and solidify liquid according to the ratio of 1:0.8~1.5, and each component of the powder is by mass percentage are as follows: calcium sulfate salt 20%~40%, synthos 5%~40%, organic additive 2%~15% and DBM bone meal 20%~60%；The calcium sulfate salt is half-H 2 O calcium sulphate and calcium sulphate dihydrate；The synthos are type alpha tricalcium phosphate, bata-tricalcium phosphate or hydroxyapatite.Bone-grafting material of the present invention has outstanding osteoinductive, osteoconductive and anti-collapsibility performance, can solidify in vivo, so that DBM is fixed on bone defect area, while DBM being made slowly to degrade, sustained release growth factor；The application can be prepared into different dosage forms by controlling liquid-solid ratio, can meet the various demands of patient.
Description
Technical field
The invention belongs to biomedical materials fields, and in particular to a kind of shaping DBM self-bone grafting self-curing bone-grafting material
And preparation method thereof.
Background technique
In the world, bone collection is the second largest tissue transplantation for being only second to blood transfusion.Currently, for treating bone defect
Three kinds of materials are mainly autologous bone, homogeneous allogenic bone and artificial bone.Wherein autologous bone is used as the goldstandard of bone collection always, controls
Therapeutic effect is best, but surgical procedure is complex, needs to take bone from patient body, leads to postoperative pain and complication.It is of the same race
Allograph bone will not additionally cause suffering to people such as decalcified bone matrix (DBM), while have preferable osteoinductive, promote skeletonization
Cell growth, but material source is insufficient, and is difficult to meet minimally-invasive treatment, needs to realize minimally invasive control by carrier of other materials
It treats.Artificial bone has from a wealth of sources, and the advantage of product diversification is, it can be achieved that minimally-invasive treatment, in conjunction with artificial bone and homogeneous allogenic bone
Advantage, a kind of shaping DBM self-bone grafting self-curing bone-grafting material can be prepared.
Calcium sulfate has good biocompatibility, osteoconductive and injectable as a kind of traditional bone renovating material
Property, more excellent achievement is clinically obtained.But that there are degradation times is too fast for calcium sulfate class bone renovating material, lacks bone
The disadvantages of induced activity.Calcium phosphate has good bioactivity, biocompatibility and syringeability, is capable of providing Human osteoblast
Required calcium ion and phosphate anion；But calcium phosphate bone-grafting material degradability is poor, hinders the growth of bone tissue；And
The prevalent means of this disadvantage are solved as production porous bio-ceramic, growing into and adsorbing conducive to osteoblast and growth factor,
But this kind of product mechanical strength is poor, while being difficult to meet minimally-invasive treatment.By the artificial bone of homogenous material preparation due to itself
The defect of material, it tends to be difficult to meet various requirements.So selecting two or more material, compound material is each
The advantage of aspect, the shortcomings that avoiding material, the requirement for reaching clinically various aspects jointly is just particularly important.
Bond material various aspects advantage, it is main that Wright company, the U.S., which has developed one kind with α-half-H 2 O calcium sulphate and DBM,
The product ALLOMATRIX Putty of raw material in order to overcome calcium sulfate to degrade too fast defect, and then has developed a kind of α-half water
Calcium sulfate, β-TCP and DBM are the product PRO-STIM of primary raw material.Such product must rely on α-sulfate hemihydrate of medical grade
Calcium, otherwise its mechanical property and degradation property can all be affected, meanwhile, wherein β-TCP can not self-curing, Jin Jinqi
To degradation speed is slowed down, the effect of calcium source phosphorus source is provided, so after calcium sulfate degradation, and what bone defect did not healed also completely
In the case of, lack the effect of mechanical support.
Summary of the invention
The present invention is in view of the deficiencies of the prior art, and it is an object of the present invention to provide a kind of shaping DBM self-bone grafting self-curing bone grafting material
Material and preparation method thereof.
For achieving the above object, the technical scheme adopted by the invention is as follows:
A kind of shaping DBM self-bone grafting self-curing bone-grafting material, by powder and solidify liquid according to the ratio of 1:0.8~1.5
Composition, each component of the powder is by mass percentage are as follows: calcium sulfate salt 20%~40%, has synthos 5%~40%
Machine additive 2%~15% and DBM bone meal 20%~60%.
In above scheme, the solidify liquid is phosphate solution, sterilized water for injection or physiological saline.
In above scheme, the calcium sulfate salt is half-H 2 O calcium sulphate and calcium sulphate dihydrate.
In above scheme, the half-H 2 O calcium sulphate is α-half-H 2 O calcium sulphate or β-half-H 2 O calcium sulphate.
In above scheme, the mass ratio of the half-H 2 O calcium sulphate and calcium sulphate dihydrate is 1:0.01~1.
In above scheme, the synthos are type alpha tricalcium phosphate, bata-tricalcium phosphate or hydroxyapatite.
In above scheme, the organic additive is sodium alginate, hyaluronic acid, chitosan, cellulose, gelatin and poly- cream
One or more of acid.
In above scheme, the DBM bone meal is allogeneic bone meal, having a size of 100 μm~900 μm.
The preparation method of above-mentioned shaping DBM self-bone grafting self-curing bone-grafting material, includes the following steps:
(1) calcium sulfate salt, synthos, organic additive are weighed, mechanical lapping is uniformly mixed at normal temperature, obtains powder
Body；
(2) a certain amount of powder and solidify liquid are taken, is mixed evenly, is starched according to solid-liquid mass ratio 1:0.8~1.5
Material；
(3) DBM particle is added in slurry uniformly mixed obtained by step (2), continues to stir, so that DBM bone meal exists
It is uniformly dispersed in mud, obtains shaping DBM self-bone grafting self-curing bone-grafting material.
In above scheme, the time of step (1) the mechanical lapping mixing is 10~120min.
In above scheme, injection-type product, pureed product or preforming can be prepared using different solid-liquid mass ratios
Product, the preformed product are by the way that by bone-grafting material, curing molding, freeze-drying are obtained under conditions of constant temperature and humidity.
The bone-grafting material that the present invention obtains is applied to Bone Defect Repari field, shaping, can be made as injection-type, pureed and pre-
Shaped article, DBM is coated in inorganic phase after product solidification.Since inorganic phase is the calcium sulfate being evenly distributed and apatite two-phase
Structure, degradation speed is slow compared with calcium sulfate, DBM can be made slowly to degrade, sustained release growth factor, induced osteogenesis.
Bone-grafting material during degradation, wherein meet preferential degradation by calcium sulfate, provides a large amount of calcium source, is formed simultaneously
A large amount of gap, promotion skeletonization and osteoblast are grown into, and at the same time, apatite can mutually play certain mechanical support
Effect, while providing calcium source and phosphate anion in the later period, guarantees the presence of calcium source during skeletonization, in this way can be to avoid
Calcium sulfate degrade too fast or apatite degraded it is slow and the shortcomings that be unfavorable for skeletonization.
Beneficial effects of the present invention: being compared to existing product, and calcium sulfate used in the present invention is not limited to medical grade α-half
H 2 O calcium sulphate has widened raw material sources, while the bone-grafting material also has the advantage that 1) there is superior bio compatibility,
Osteoconductive and osteoinductive；2) bone-grafting material being capable of successive induction skeletonization；3) the application can be prepared by liquid-solid ratio
At different dosage forms, wherein injection-type can be used for minimally invasive, pureed product can random-shaping, the various demands of patient can be met；4)
The injectable time of injection-type product of the present invention is greater than 20min, can satisfy clinical demand, and mix simply, conveniently faces
Bed operation；5) injection-type product syringeability of the present invention is good, has preferable anti-collapsibility performance；6) bone grafting of the present invention
Material energy in-situ solidifying plays the role of certain mechanical support, and later period calcium sulfate preferential degradation, is capable of forming porous structure,
On the one hand bone conduction effect can be played, on the other hand facilitates growing into for osteoblast, rapid bone formation.
Detailed description of the invention
Fig. 1 is Histological section's figure of bone-grafting material dystopy induced osteogenesis of the present invention experiment, wherein (a) is bone grafting material
Material is implanted into 3 weeks Histological section's figures (HE × 40) in rat muscle, and 6 weeks groups (b) are implanted into rat muscle for bone-grafting material
It knits and learns slice map (HE × 40), (c) be implanted into 12 weeks Histological sections in rat muscle for bone-grafting material and scheme (HE × 40).
Fig. 2 is that the Histological section of bone-grafting material Rat calvarial implantation experiment of the present invention schemes, wherein (a) is rat cranium
The picture at bone implant (6 weeks) position is (b) Histological section of implant site, (c) schemes for the Histological section of HE × 40.
Fig. 3 is that bone-grafting material of the present invention is injected into the pattern in physiological saline, wherein (a) is to be injected into physiological saline
In, it is (b) pattern after placement 1 day.
Fig. 4 is that bone-grafting material of the present invention is degraded the XRD curve graph after different time in physiological saline.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment content that the present invention is furture elucidated, but it is of the invention
Content is not limited solely to the following examples.
Embodiment 1
A kind of shaping DBM self-bone grafting self-curing bone-grafting material (injection-type), is prepared via a method which method: by β-half
20 parts of H 2 O calcium sulphate, 10 parts of calcium sulphate dihydrate, 30 parts of α-TCP, 5 parts of sodium carboxymethylcellulose, by mechanical lapping 20min, make
Powder is uniformly mixed, and is then added 2wt% disodium phosphate soln according to solid-to-liquid ratio 1:1, is stirred into pureed, then adds 35 parts
DBM, the DBM bone meal is allogeneic bone meal, having a size of 100 μm~900 μm, continues to stir 1min, DBM is made to be uniformly dispersed.
It transfers the sample into syringe, is injected into physiological saline, placement one day not defeated and dispersed；It is injected into mold, is then put into perseverance
Curing molding in constant temperature and humidity case (37 DEG C of temperature, humidity 98%), after solidifying 3d, compression strength 3.2MPa.
Embodiment 2
A kind of shaping DBM self-bone grafting self-curing bone-grafting material (face chamotte mould), is prepared via a method which method: by α-half
39 parts of H 2 O calcium sulphate, 1 part of calcium sulphate dihydrate, 20 parts of β-TCP, 5 parts of hydroxypropyl methyl cellulose, by mechanical lapping 15min,
It is uniformly mixed powder, then water for injection is added according to solid-to-liquid ratio 1:0.9, stirs into pureed, then adds 35 parts of DBM, institute
Stating DBM bone meal is allogeneic bone meal, having a size of 100 μm~900 μm, continues to stir 1min, DBM is made to be uniformly dispersed.By sample
It is transferred in syringe, is injected into physiological saline, placement one day not defeated and dispersed；It is injected into mold, is then put into constant temperature and humidity
Curing molding in case (37 DEG C of temperature, humidity 98%), after solidifying 3d, compression strength 3.0MPa.
Embodiment 3
A kind of shaping DBM self-bone grafting self-curing bone-grafting material (preforming), is prepared via a method which method: by β-half
20 parts of H 2 O calcium sulphate, 10 parts of calcium sulphate dihydrate, 30 parts of α-TCP, 5 parts of sodium carboxymethylcellulose, by mechanical lapping 20min, make
Powder is uniformly mixed, and is then added 2wt% disodium phosphate soln according to solid-to-liquid ratio 1:1, is stirred into pureed, then adds 35 parts
DBM, the DBM bone meal is allogeneic bone meal, having a size of 100 μm~900 μm, continues to stir 1min, DBM is made to be uniformly dispersed.
It fills a sample into mold, the curing molding in climatic chamber (37 DEG C of temperature, humidity 98%), it is chilled after solidifying 3d
It is dry, obtain preformed product, compression strength 3.9MPa.
Embodiment 4
A kind of shaping DBM self-bone grafting self-curing bone-grafting material (injection-type), is prepared via a method which method: by α-half
15 parts of H 2 O calcium sulphate, 10 parts of calcium sulphate dihydrate, 25 parts of α-TCP, 1 part of Sodium Hyaluronate, 6 parts of hydroxypropyl methyl cellulose, pass through
Mechanical lapping 20min is uniformly mixed powder, then adds 3wt% disodium phosphate soln, stirring according to solid-to-liquid ratio 1:1.1
At pureed, 43 parts of DBM are then added, the DBM bone meal is that allogeneic bone meal continues to stir having a size of 100 μm~900 μm
1min makes DBM be uniformly dispersed.It transfers the sample into syringe, is injected into physiological saline, placement one day not defeated and dispersed；Injection
Into mold, it is then put into curing molding in climatic chamber (37 DEG C of temperature, humidity 98%), after solidifying 3d, compression strength is
2.5MPa。
Embodiment 5
A kind of shaping DBM self-bone grafting self-curing bone-grafting material (injection-type), is prepared via a method which method: by α-half
20 parts of H 2 O calcium sulphate, 15 parts of calcium sulphate dihydrate, 29 parts of α-TCP, 6 parts of hydroxypropyl methyl cellulose, by mechanical lapping 20min,
It is uniformly mixed powder, then 3wt% disodium phosphate soln is added according to solid-to-liquid ratio 1:0.9, stirs into pureed, then add
30 parts of DBM, the DBM bone meal is allogeneic bone meal, having a size of 100 μm~900 μm, continues to stir 1min, keeps DBM dispersion equal
It is even.It transfers the sample into syringe, is injected into physiological saline, placement one day not defeated and dispersed；It is injected into mold, is then put into
Curing molding in climatic chamber (37 DEG C of temperature, humidity 98%), after solidifying 3d, compression strength 4.8MPa.
Fig. 1 is Histological section's figure of bone-grafting material dystopy induced osteogenesis of the present invention experiment, wherein (a) is bone grafting material
Material is implanted into 3 weeks Histological section's figures (HE × 40) in rat muscle, and 6 weeks groups (b) are implanted into rat muscle for bone-grafting material
It knits and learns slice map (HE × 40), (c) be implanted into 12 weeks Histological sections in rat muscle for bone-grafting material and scheme (HE × 40).From figure
In it can be seen that implantation 3 weeks after, osteoblast can be observed；After being implanted into 6 weeks, osteocyte and marrow, bone-grafting material can be observed
Skeletonization is obvious；After being implanted into 12 weeks, observable cartilage cell illustrates that material being capable of successive induction skeletonization.
Fig. 2 is skull implant site picture and the Histological section of bone-grafting material Rat calvarial implantation experiment of the present invention
Figure, wherein (a) is the picture that Rat calvarial is implanted into (6 weeks) position, it can be seen that material is completely and calvarial fusion, skeletonization effect are aobvious
It writes, (b) is the Histological section of implant site, it will also be seen that material merges completely with skull, it (c) is the tissue of HE × 40
Slice map is learned, it can be observed that a large amount of osteoblast and osteocyte, embody good skeletonization effect.
Fig. 3 is the pattern (b) after bone-grafting material of the present invention is injected into (a) in physiological saline and places 1 day, Cong Tuzhong
As can be seen that sample can be injected uniformly, do not have defeated and dispersed after placing one day, there is good anti-collapsibility performance.
Fig. 4 is that bone-grafting material described in the invention patent is degraded the XRD curve graph after different time in physiological saline, from figure
In as can be seen that calcium sulfate is degradable at 28 days, be only left synthos.
Bone-grafting material of the present invention has good self-bone grafting ability, promotes skeletonization due to containing DBM.Calcium sulfate and
On the one hand the presence of calcium phosphate can provide calcium source and phosphate anion, help skeletonization；On the other hand play the role of osteoacusis,
Calcium sulfate meeting preferential degradation, forms a large amount of gaps, promotes growing into for osteoblast；Meanwhile remaining phosphate can not only continue
DBM is coated, the degradation speed of DBM is slowed down, makes its slow release growth factor, successive induction skeletonization can also continue to provide calcium source
And phosphate anion, prevent the too fast formation hole of material degradation.
The application is prepared into different dosage forms by adjusting liquid-solid ratio, and wherein injection-type can be used for minimally invasive, and pureed product can appoint
Meaning moulding, can meet the various demands of patient；The curing time of injection-type product of the present invention is greater than 20min, can satisfy
Clinical demand, and mix simply, facilitate clinical manipulation；Injection-type product syringeability of the present invention is good, has preferable anti-
Collapsibility performance.
Obviously, above-described embodiment is only intended to clearly illustrate made example, and is not the limitation to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation or change therefore amplified
It moves within still in the protection scope of the invention.
Claims (9)
1. a kind of shaping DBM self-bone grafting self-curing bone-grafting material, which is characterized in that by powder and solidify liquid according to 1:0.8 ~
1.5 ratio composition, each component of the powder is by mass percentage are as follows: and calcium sulfate salt 20% ~ 40%, synthos 5% ~
40%, organic additive 2% ~ 15% and DBM bone meal 20% ~ 60%.
2. shaping DBM self-bone grafting self-curing bone-grafting material according to claim 1, which is characterized in that the solidify liquid
For phosphate solution, water for injection or physiological saline.
3. shaping DBM self-bone grafting self-curing bone-grafting material according to claim 1, which is characterized in that the calcium sulfate
Salt is half-H 2 O calcium sulphate and calcium sulphate dihydrate.
4. shaping DBM self-bone grafting self-curing bone-grafting material according to claim 1, which is characterized in that the calcium phosphate
Salt is type alpha tricalcium phosphate, bata-tricalcium phosphate or hydroxyapatite.
5. shaping DBM self-bone grafting self-curing bone-grafting material according to claim 1, which is characterized in that described organic to add
Adding agent is one or more of sodium alginate, hyaluronic acid, chitosan, cellulose, gelatin and polylactic acid.
6. shaping DBM self-bone grafting self-curing bone-grafting material according to claim 1, which is characterized in that the DBM bone meal
For allogeneic bone meal, having a size of 100 μm ~ 900 μm.
7. the preparation method of any shaping DBM self-bone grafting self-curing bone-grafting material, feature exist in claim 1 ~ 6
In including the following steps:
(1) calcium sulfate salt, synthos, organic additive are weighed；Mechanical lapping is uniformly mixed at normal temperature, obtains powder；
(2) a certain amount of powder and solidify liquid are taken, is mixed evenly according to solid-liquid mass ratio 1:0.8 ~ 1.5, obtains slurry；
(3) DBM particle is added in slurry obtained by step (2), continues to stir, so that DBM bone meal is uniformly dispersed in the slurry,
Obtain shaping DBM self-bone grafting self-curing bone-grafting material.
8. preparation method according to claim 7, which is characterized in that the time of step (1) mechanical lapping mixing is
10~120min。
9. preparation method according to claim 7, which is characterized in that can be made into injection-type production using different solid-to-liquid ratios
Product, pureed product or preformed product, the preformed product are by solidifying bone-grafting material under conditions of constant temperature and humidity
Molding, freeze-drying obtain.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110339394A (en) * | 2019-08-01 | 2019-10-18 | 陶合体科技(苏州)有限责任公司 | A kind of material preparing bone defect repair support and preparation method |
| CN112023120A (en) * | 2020-09-10 | 2020-12-04 | 湖北联结生物材料有限公司 | Injectable pre-filled bone repair particle and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091351A (en) * | 2011-01-14 | 2011-06-15 | 北京大清生物技术有限公司 | Injectable bone repair material |
| US20140342013A1 (en) * | 2013-03-14 | 2014-11-20 | Skeletal Kinetics, Llc | Calcium phosphate cement compositions that set into high strength porous structures |
| CN108379652A (en) * | 2018-04-28 | 2018-08-10 | 湖北联结生物材料有限公司 | Have both induced degradation characteristic bone cement and preparation method thereof |
-
2019
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091351A (en) * | 2011-01-14 | 2011-06-15 | 北京大清生物技术有限公司 | Injectable bone repair material |
| US20140342013A1 (en) * | 2013-03-14 | 2014-11-20 | Skeletal Kinetics, Llc | Calcium phosphate cement compositions that set into high strength porous structures |
| CN108379652A (en) * | 2018-04-28 | 2018-08-10 | 湖北联结生物材料有限公司 | Have both induced degradation characteristic bone cement and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110339394A (en) * | 2019-08-01 | 2019-10-18 | 陶合体科技(苏州)有限责任公司 | A kind of material preparing bone defect repair support and preparation method |
| CN112023120A (en) * | 2020-09-10 | 2020-12-04 | 湖北联结生物材料有限公司 | Injectable pre-filled bone repair particle and preparation method and application thereof |
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Application publication date: 20190709 |