CN106620872A - Formula and preparation method of engineered bone scaffold - Google Patents
Formula and preparation method of engineered bone scaffold Download PDFInfo
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- CN106620872A CN106620872A CN201710036720.7A CN201710036720A CN106620872A CN 106620872 A CN106620872 A CN 106620872A CN 201710036720 A CN201710036720 A CN 201710036720A CN 106620872 A CN106620872 A CN 106620872A
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- Prior art keywords
- polyvinyl alcohol
- calcium phosphate
- bone
- biphasic calcium
- powder
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000009472 formulation Methods 0.000 title description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 47
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 47
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 32
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 32
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 32
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 32
- 239000000843 powder Substances 0.000 claims abstract description 31
- 210000001519 tissue Anatomy 0.000 claims abstract description 18
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 16
- 239000011575 calcium Substances 0.000 claims abstract description 16
- 239000000084 colloidal system Substances 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 15
- 239000012620 biological material Substances 0.000 claims abstract description 14
- 230000011164 ossification Effects 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 11
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 11
- 230000002051 biphasic effect Effects 0.000 claims description 43
- 230000007547 defect Effects 0.000 claims description 24
- 239000013521 mastic Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 8
- 238000010257 thawing Methods 0.000 claims description 8
- 238000006136 alcoholysis reaction Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- UUVBYOGFRMMMQL-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca].OP(O)(O)=O UUVBYOGFRMMMQL-UHFFFAOYSA-N 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 13
- 239000003102 growth factor Substances 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000013329 compounding Methods 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000010146 3D printing Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 239000002131 composite material Substances 0.000 description 7
- 229920002521 macromolecule Polymers 0.000 description 4
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 150000002085 enols Chemical class 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 3
- 150000002894 organic compounds Chemical class 0.000 description 3
- 230000004072 osteoblast differentiation Effects 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000011337 individualized treatment Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000000399 orthopedic effect Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 108010048734 sclerotin Proteins 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Abstract
The invention discloses a formula and a preparation method of an engineered bone scaffold. The formula comprises polyvinyl alcohol powder, deionized water and two-phase calcium phosphate. Firstly, 0.15 g/ml of polyvinyl alcohol colloid is prepared by dissolving polyvinyl alcohol in deionized water, then two-phase calcium phosphate-polyvinyl alcohol paste tissue is formed by compounding two-phase calcium phosphate and polyvinyl alcohol colloid, the paste as a raw material is printed by a biological material printer, and a two-phase calcium phosphate-polyvinyl alcohol tissue engineered bone scaffold material with inducing osteogenesis capacity is prepared. The bone tissue engineering scaffold can combine growth factors and medicines, has a slow release effect and has effective potential clinical use value.
Description
Technical field
The invention belongs to pharmaceutical technology field, more particularly to the formula and preparation method of Engineering Bone support.
Background technology
Cranial defect, bone does not connect on a large scale, are very common problems in curative activity.Be common in wound, tumor, infection,
Congenital or developmental character disease etc..Which is always a global problem of field of orthopaedics, not yet obtains effectively solving so far.Mesh
Front conventional restorative procedure has autologous bone transplanting, allogenic bone transplantation, synthetic substitute etc., but these methods in having for area
Sex-limited, more complication and sequela, antigenicity, biocompatibility, biological activity, biodegradability and with host bone
The aspects such as mechanical matching, service life all have respective shortcoming, limit its clinical practice, it is difficult to reach satisfied effect.Group
Knit the proposition of engineering, set up and development be to tissue in surgical field, organ defect and dysfunction traditional therapy and
The revolution of pattern.It is brand-new Therapeutic mode using tissue engineering ways and means repair tissue defect, with wide
Application prospect, be the hot issue of current medical circle research；Its final purpose is that preferable offer, wide material sources, answers
The bioactivity osseous tissue determined with easy, curative effect, bone tissue engineer are wherein particularly important with the neck for representing meaning
Domain, for the therapeutic effect of the diseases such as Cranial defect that various clinical common causes are caused fundamentally is improved, osseous tissue work
Journey breaches conventional Therapeutic mode, there is provided brand-new thinking and method.
Due to being presented irregularly shaped Cranial defect shape, external preforming bone-grafting material is difficult in adapt to Cranial defect shape more,
Interval is formed between normal sclerotin, so as to affect tissue engineered bone support conduction, the appearance of 3D printing technique can be fine
Solution individualized treatment this difficult problem.3D printing technique is referred to as " idea factories on desktop ".First will be produced with related software
Product drawing is converted into 3D rendering data and uploads computer, after being put into corresponding material, printing head will according to view data by
Thing is printed by layer, then is stacked and is become a stereo article.Due to adopting " addition manufacturing technology " (additive
Manufacturing raw material and artificial), therefore is more saved, and the article for coming in every shape can be made.In theory, as long as
The moulding that computer can be designed that, 3D printer can print out.Tele-medicine, personalized medicine are the development of World Medical
Trend.3D printing technique can produce various artificial organs and tissue using different " ink " materials, can meet clinical trouble
Person is individual to be required.Metal, resin, Gypsum Fibrosum, ceramics, macromolecule, biomacromolecule etc. be creatively applied to 3D printing with
Production biological product.With medical skill individualized development, technique is widely used for making accurately suffers from bone model, spends body
The bone defect healing body of individuation customized.Biomaterial printer is state-of-the-art without silk printing technique using the world of original creation, can
The parameters such as Cranial defect size, shape and internal voids according to Orthopedic Clinical patient, accurately customize out physicochemical characteristicses and outward appearance knot
The bionical biodegradable artificial bone of structure.
Bone tissue engineering stent material composite growth factor or medicine can improve implantation timbering material Bone Defect Repari effect,
But in clinical case, the bony defect of required reparation is often larger, defect perilesional is susceptible to infection, and single is given
Give the somatomedin or medicine of heavy dose to be difficult to play preferable effect, or even be harmful to body.So, in addition it is also necessary to growth
The factor or medicine carry out slow release.Existing result of study shows that polyvinyl alcohol colloid has a good slow release effect, and
The application having in terms of slow release, therefore available polyethylene alcohol colloid prepares tissue engineered bone support as binding agent and slow-released carrier is combined
Somatomedin and medicine implantation Cranial defect local play a role.
Therefore, develop and can print via 3D printer and bionical have by the imaging data of Cranial defect patient
The biodegradable artificial bone of the sustained release growth factor and medicine, and the bone tissue engineering stent material of supporting role is produced with weight
Want meaning.
The content of the invention
The technical problem to be solved is：The formula and preparation method of Engineering Bone support, the present invention are provided
Cranial defect individual is solved well treats this difficult problem.
To solve above-mentioned technical problem, the technical scheme is that：The formula of Engineering Bone support, it is characterised in that：Adopt
Use formula as below：Using formula as below：Pva powder, deionized water, biphasic calcium phosphate powder, described polyvinyl alcohol powder
Last alcoholysis degree is 99+%.
2nd, the preparation method of Engineering Bone support according to claim 1, it is characterised in that：Carry out according to the following steps：
(1) it is made into：Pva powder is dissolved in into the polyvinyl alcohol colloid that deionized water is made into 0.15g/ml first；
(2) mix：Polyvinyl alcohol colloid in (1) is mixed with biphasic calcium phosphate powder；
(3) stir：Complex in (2) is stirred, biphasic calcium phosphate and polyvinyl alcohol mastic line and staff control is obtained；
(4) it is loaded into：Patient bone defect model three dimensional file is loaded into into biomaterial printer；
(5) print：Biphasic calcium phosphate will be obtained and biomaterial printer will be poured into polyvinyl alcohol mastic line and staff control, then
Printed by biomaterial printer and the biphasic calcium phosphate-polyvinyl alcohol tissue engineered bone with induced osteogenesis ability is obtained
Frame material.
(6) freeze thawing：The biphasic calcium phosphate that (5) are drawn-polyvinyl alcohol bone tissue engineering scaffold carries out subzero 20
Degree, the frost of 10～20 hours or so, then the room temperature defrosting of 10～20 hours.
(7) it is dried；By the biphasic calcium phosphate after (6) freeze thawing-polyvinyl alcohol bone tissue engineering scaffold lyophilization
Machine is dried 2～5 hours, i.e. Engineering Bone rack forming.
Further, in described (2) blend step, biphasic calcium phosphate powder and polyvinyl alcohol colloid are according to 1g:1.5g ratio
It is compounded to form biphasic calcium phosphate-polyvinyl alcohol mastic tissue.
Pva powder：Organic compound, extremely safe macromolecule organic, with good biocompatibility.
Deionized water：For fusion agent, high-purity water.
Biphasic calcium phosphate powder：For bone alternate material, with good biocompatibility.
The formula and preparation method of the Engineering Bone support, it is empty by the Cranial defect size of clinical patients, shape and inside
The parameters such as gap, accurately customize out the bionical biodegradable artificial bone of physicochemical characteristicses and surface structure, prepare with ossification
3D printing biphasic calcium phosphate-polyvinyl alcohol bone tissue engineering scaffold, and the tissue engineered bone support can composite growth because
Son and medicine simultaneously reach the effect of slow release, it has been investigated that the 3D printing biphasic calcium phosphate-poly- second built according to this component ratio
Enol bone tissue engineering scaffold has significant rush ossification, can surrounding be produced into mesenchymal stem cells MSCs
Change is acted on, and can induce these cell Osteoblast Differentiation, finally plays ossification, and can with composite growth factor and medicine,
The effect of slow release is reached, the present invention can be very good solution Cranial defect individual and treat this difficult problem.
Description of the drawings
Fig. 1 is preparation flow figure of the present invention
Specific embodiment
Hereinafter, various specific details are elaborated, to provide the saturating of the concept to constituting described embodiment basis
Thorough understanding.However, it will be apparent to those skilled in the art that described embodiment can not have these specific details
In it is some or all in the case of putting into practice.In other cases, well-known process step is not specifically described.
Embodiment 1
The formula of the Engineering Bone support that the 1st, the present embodiment is enumerated, is made up of the raw material of following weight：Pva powder, go
Ionized water, biphasic calcium phosphate powder, described pva powder alcoholysis degree are 99+%.
2nd, the preparation method of Engineering Bone support according to claim 1, it is characterised in that：Carry out according to the following steps：
(1) it is made into：Pva powder is dissolved in into the polyvinyl alcohol colloid that deionized water is made into 0.15g/ml first；
(2) mix：Polyvinyl alcohol colloid in (1) is mixed with biphasic calcium phosphate powder；
(3) stir：Complex in (2) is stirred, biphasic calcium phosphate and polyvinyl alcohol mastic line and staff control is obtained；
(4) it is loaded into：Patient bone defect model three dimensional file is loaded into into biomaterial printer；
(5) print：Biphasic calcium phosphate will be obtained and biomaterial printer will be poured into polyvinyl alcohol mastic line and staff control, then
Printed by biomaterial printer and the biphasic calcium phosphate-polyvinyl alcohol tissue engineered bone with induced osteogenesis ability is obtained
Frame material.
(6) freeze thawing：The biphasic calcium phosphate that (5) are drawn-polyvinyl alcohol bone tissue engineering scaffold carries out subzero 20
Degree, the frost of 10 hours or so, then the room temperature defrosting of 10 hours.
(7) it is dried；By the biphasic calcium phosphate after (6) freeze thawing-polyvinyl alcohol bone tissue engineering scaffold lyophilization
Machine is dried 2 hours, i.e. Engineering Bone rack forming.
Further, in described (2) blend step, biphasic calcium phosphate powder and polyvinyl alcohol colloid are according to 1g:1.5g ratio
It is compounded to form biphasic calcium phosphate-polyvinyl alcohol mastic tissue.
Pva powder：Organic compound, extremely safe macromolecule organic, with good biocompatibility.
Deionized water：For fusion agent, high-purity water.
Biphasic calcium phosphate powder：For bone alternate material, with good biocompatibility.
The formula and preparation method of the Engineering Bone support, it is empty by the Cranial defect size of clinical patients, shape and inside
The parameters such as gap, accurately customize out the bionical biodegradable artificial bone of physicochemical characteristicses and surface structure, prepare with ossification
3D printing biphasic calcium phosphate-polyvinyl alcohol bone tissue engineering scaffold, and the tissue engineered bone support can composite growth because
Son and medicine simultaneously reach the effect of slow release, it has been investigated that the 3D printing biphasic calcium phosphate-poly- second built according to this component ratio
Enol bone tissue engineering scaffold has significant rush ossification, can surrounding be produced into mesenchymal stem cells MSCs
Change is acted on, and can induce these cell Osteoblast Differentiation, finally plays ossification, and can with composite growth factor and medicine,
The effect of slow release is reached, the present invention can be very good solution Cranial defect individual and treat this difficult problem.
Embodiment 2
The formula of the Engineering Bone support that the 1st, the present embodiment is enumerated, is made up of the raw material of following weight：Pva powder, go
Ionized water, biphasic calcium phosphate powder, described pva powder alcoholysis degree are 99+%.
2nd, the preparation method of Engineering Bone support according to claim 1, it is characterised in that：Carry out according to the following steps：
(1) it is made into：Pva powder is dissolved in into the polyvinyl alcohol colloid that deionized water is made into 0.15g/ml first；
(2) mix：Polyvinyl alcohol colloid in (1) is mixed with biphasic calcium phosphate powder；
(3) stir：Complex in (2) is stirred, biphasic calcium phosphate and polyvinyl alcohol mastic line and staff control is obtained；
(4) it is loaded into：Patient bone defect model three dimensional file is loaded into into biomaterial printer；
(5) print：Biphasic calcium phosphate will be obtained and biomaterial printer will be poured into polyvinyl alcohol mastic line and staff control, then
Printed by biomaterial printer and the biphasic calcium phosphate-polyvinyl alcohol tissue engineered bone with induced osteogenesis ability is obtained
Frame material.
(6) freeze thawing：The biphasic calcium phosphate that (5) are drawn-polyvinyl alcohol bone tissue engineering scaffold carries out subzero 20
Degree, the frost of 20 hours or so, then the room temperature defrosting of 20 hours.
(7) it is dried；By the biphasic calcium phosphate after (6) freeze thawing-polyvinyl alcohol bone tissue engineering scaffold lyophilization
Machine is dried 5 hours, i.e. Engineering Bone rack forming.
Further, in described (2) blend step, biphasic calcium phosphate powder and polyvinyl alcohol colloid are according to 1g:1.5g ratio
It is compounded to form biphasic calcium phosphate-polyvinyl alcohol mastic tissue.
Pva powder：Organic compound, extremely safe macromolecule organic, with good biocompatibility.
Deionized water：For fusion agent, high-purity water.
Biphasic calcium phosphate powder：For bone alternate material, with good biocompatibility.
The formula and preparation method of the Engineering Bone support, it is empty by the Cranial defect size of clinical patients, shape and inside
The parameters such as gap, accurately customize out the bionical biodegradable artificial bone of physicochemical characteristicses and surface structure, prepare with ossification
3D printing biphasic calcium phosphate-polyvinyl alcohol bone tissue engineering scaffold, and the tissue engineered bone support can composite growth because
Son and medicine simultaneously reach the effect of slow release, it has been investigated that the 3D printing biphasic calcium phosphate-poly- second built according to this component ratio
Enol bone tissue engineering scaffold has significant rush ossification, can surrounding be produced into mesenchymal stem cells MSCs
Change is acted on, and can induce these cell Osteoblast Differentiation, finally plays ossification, and can with composite growth factor and medicine,
The effect of slow release is reached, the present invention can be very good solution Cranial defect individual and treat this difficult problem.
The present invention is not limited to above-mentioned specific embodiment, one of ordinary skill in the art from above-mentioned design,
Without performing creative labour, a variety of conversion made, it is within the scope of the present invention.
Claims (3)
1. the formula of Engineering Bone support, it is characterised in that：Using formula as below：Pva powder, deionized water, two-phase phosphoric acid
Calcium powder, described pva powder alcoholysis degree are 99+%.
2. the preparation method of the Engineering Bone support described in claim 1 is prepared, it is characterised in that：Carry out according to the following steps：
(1) it is made into：Pva powder is dissolved in into the polyvinyl alcohol colloid that deionized water is made into 0.15g/ml first；
(2) mix：Polyvinyl alcohol colloid in (1) is mixed with biphasic calcium phosphate powder；
(3) stir：Complex in (2) is stirred, biphasic calcium phosphate and polyvinyl alcohol mastic line and staff control is obtained；
(4) it is loaded into：Patient bone defect model three dimensional file is loaded into into biomaterial printer；
(5) print：Biphasic calcium phosphate in (3) is poured into biomaterial printer with polyvinyl alcohol mastic line and staff control, Ran Houtong
Cross biomaterial printer and print prepared biphasic calcium phosphate-polyvinyl alcohol tissue engineered bone support with induced osteogenesis ability
Material.
(6) freeze thawing：The biphasic calcium phosphate that (5) are drawn-polyvinyl alcohol bone tissue engineering scaffold carries out minus 20 degrees, and 10
The frost of～20 hours or so, then the room temperature defrosting of 10～20 hours.
(7) it is dried；Biphasic calcium phosphate after (6) freeze thawing-polyvinyl alcohol bone tissue engineering scaffold is dry with freezer dryer
Dry 2～5 hours, i.e. Engineering Bone rack forming.
3. the preparation method of Engineering Bone support according to claim 2, it is characterised in that double in described (2) blend step
Calcium phosphate phase powder is with polyvinyl alcohol colloid according to 1g:1.5g ratios are compounded to form biphasic calcium phosphate-polyvinyl alcohol mastic group
Knit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710036720.7A CN106620872B (en) | 2017-01-18 | 2017-01-18 | The formula and preparation method of Engineering Bone bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710036720.7A CN106620872B (en) | 2017-01-18 | 2017-01-18 | The formula and preparation method of Engineering Bone bracket |
Publications (2)
| Publication Number | Publication Date |
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| CN106620872A true CN106620872A (en) | 2017-05-10 |
| CN106620872B CN106620872B (en) | 2019-07-19 |
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| CN201710036720.7A Expired - Fee Related CN106620872B (en) | 2017-01-18 | 2017-01-18 | The formula and preparation method of Engineering Bone bracket |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108478873A (en) * | 2018-04-25 | 2018-09-04 | 信阳师范学院 | A kind of preparation method of biphasic calcium phosphate plural gel |
| CN108478882A (en) * | 2018-04-25 | 2018-09-04 | 信阳师范学院 | A kind of preparation method of compound bone holder material |
| CN109133907A (en) * | 2018-08-16 | 2019-01-04 | 迈海新型材料科技（固安）有限公司 | A kind of artificial bone and preparation method thereof comprising hydroxyapatite crystal whisker and biphase calcium phosphor |
| CN115463254A (en) * | 2022-09-06 | 2022-12-13 | 点云生物(杭州)有限公司 | Spongy bone-like defect filler and preparation method thereof |
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| CN101721740A (en) * | 2008-10-16 | 2010-06-09 | 上海国睿生命科技有限公司 | Bone tissue engineering bracket material and preparation method and application thereof |
| WO2015072806A1 (en) * | 2013-11-18 | 2015-05-21 | 고려대학교산학협력단 | Double porous synthetic bone wedge and method for manufacturing same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109133907A (en) * | 2018-08-16 | 2019-01-04 | 迈海新型材料科技（固安）有限公司 | A kind of artificial bone and preparation method thereof comprising hydroxyapatite crystal whisker and biphase calcium phosphor |
| CN115463254A (en) * | 2022-09-06 | 2022-12-13 | 点云生物(杭州)有限公司 | Spongy bone-like defect filler and preparation method thereof |
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