CN106344214A - Method for preparing implantable bone grafts by de-organification and 3D printing of animal bones - Google Patents
Method for preparing implantable bone grafts by de-organification and 3D printing of animal bones Download PDFInfo
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- CN106344214A CN106344214A CN201610737279.0A CN201610737279A CN106344214A CN 106344214 A CN106344214 A CN 106344214A CN 201610737279 A CN201610737279 A CN 201610737279A CN 106344214 A CN106344214 A CN 106344214A
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- Prior art keywords
- bone
- graft
- printing
- implantable
- organise
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/28—Bones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
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- Health & Medical Sciences (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Cardiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a method for preparing implantable bone grafts by de-organification and 3D printing of animal bones. The method comprises the steps of de-organification, adhesive injection and reinforcement as well as 3D molding printing. Besides, the method is simple and easy, suitable for orthopedic bone defect repair, bone tumor, spine graft fusion surgery and other areas requiring bone graft materials. Also, the bone graft is characterized by wide range of raw materials, low cost, micro-structure similar to human skeleton, good features for promotion of osteogenesis, osteoinductivity and osteoconductivity, complete degradation in human body as well as no rejection, and consequentlyenjoys a bright market perspective.
Description
Technical field
The invention belongs to tissue biomaterial technical field is and in particular to one kind goes, using animal skeleton, the 3d printing that organises
The method preparing implantable bone graft.
Background technology
Bone, as the maximum histoorgan of human body, carries the important duty of vital movement, is but easiest to cause defect, often
There are about millions of osseous tissue defect patients year needs to be treated surgically.In China it is estimated that annual because of vehicle accident and life
Caused by product security incident, trauma fracture, spinal column degenerative disease and the orthopaedic disease such as bone tumor, bone tuberculosis, cause Cranial defect or work(
Impaired patients can reach millions of people.Bone collection has become the graft having the call being only second to transfuse blood.How to find more
How preferably osteanagenesiss repair materials, it has also become what the numerous scientist in the whole world was common pursues dynamic with diligent research
Power.
Bone graft needs there is promotion skeletonization, self-bone grafting, the characteristic of bone conduction.Current bone graft source mainly has
Nature bone and artificial bone two big class.Nature bone mainly has autologous bone, homogeneous allogenic bone and bone- xenograft.Artificial bone is mainly hydroxyl
Apatite, Calcium Carbonate and Corallium Japonicum Kishinouye bone etc..Autologous bone, homogeneous allogenic bone major defect face it is impossible to fully meet for limited source
Bed needs.The problems such as degradation rate in human body for the artificial bone, degradation speed, is not also fully solved.Bone- xenograft is mainly animal bone
Bone, it is similar in the composition of inorganic matters, content etc. to skeleton, and its micro structure is similar to skeleton, has good rush
Enter the characteristic of skeletonization, self-bone grafting, bone conduction, can be degradable in human body, substituted by itself osseous tissue, and its source is wide
General, with low cost.So being used allograph bone to have broad prospects as bone graft.
It is fabricated to fritter or little strip the bone xenograft thing using at present more, tend not in Clinical practice well
The shape adapting to patient's needs bone grafting region is it is impossible to be sufficient filling with defect area.With the development of 3d printing technique, various complexity
The making of stereochemical structure is possibly realized.
For this reason, it is proposed that a kind of manufacture method of new allogenic bone transplantation material, it comprises the process of animal skeleton, glues
The use of mixture and 3d printing and making.
Content of the invention
It is an object of the invention to provide a kind of prepare implantable bone graft using animal skeleton 3d printing of going to organise
Method, the method can meet the labyrinth in different patient's difference Cranial defect regions, and what replacement used at present can not be in human body
Other degradable bone grafts, promote the reparation of patient's osseous tissue.
The present invention is achieved through the following technical solutions the object of the invention:
Go to organise using animal skeleton calcining, original skeleton is fabricated to diameter 1 thin by organic matter removal in skeleton simultaneously
Following little particle；Binding agent injection reinforcing process in, using biological adhesive (include shitosan, collagen, Fibrin Glue,
Polylactic acid) with front step go to organise after osseous granuleses carry out in proportion being mixed into the mixture of certain viscosity；By in the preoperative
Patient is needed implant part carry out 3-D scanning, draw the stereomodel of bone graft using mapping software, then adopt 3d
Printer prints corresponding implantable bone graft.
Specifically comprise the following steps that
(1) take fresh animal bone to remove soft tissue, cleaned with normal saline, be placed at 1000 DEG C -1100 DEG C and carry out calcining 3-4h
Afterwards, pulverize and osseous granuleses are obtained；
(2) binding agent is heated to 50 DEG C -70 DEG C and is allowed to be formed pasty state, then enter with osseous granuleses 1:3-1:5 ratio in mass ratio
Row mixing；
(3) utilize mapping software to draw the stereomodel of bone graft, be printed as painting by step (2) mixture using 3d printer
The stereomodel of system, obtains final product implantable bone graft after cooling.
It it is critical only that:
In selection and process first to animal skeleton, condition is: using the Os Bovis seu Bubali close with people's skeleton, Os Caprae seu Ovis or Os Sus domestica etc.
Material, thoroughly removes soft tissue, is then cleaned with normal saline；
Secondly in the preparation process to bone meal granule, entered high temperature furnace, by being linearly heated up to 1000 DEG C -1100 DEG C, formed
Diameter is less than 1 millimeter of granule, in the process it is noted that monitoring to temperature, if too high can lead to granule carbonization；
Injection process to binding agent again, (includes shitosan, collagen, Fibrin Glue, polylactic acid etc. multiple using binding agent
Can material selection), binding agent is heated to 50 DEG C -70 DEG C and is allowed to be formed pasty state, then press 1:3-1:5 with the bone meal granule after calcining
Ratio is mixed, and this material is carried out with wire drawing process simultaneously, is formed long thread, prints phase using 3d printer after cooling
The implantable bone graft answered.
The advantage of the inventive method and technique effect:
Method is simple it is adaptable to orthopaedics bone defect healing, bone tumor, spine graft fusion operation etc. need to use bone collection
The field of thing material, and raw material sources are extensively, with low cost, it is similar in the composition of inorganic matters, content etc. to skeleton, its
Micro structure is similar to skeleton, has good promotion skeletonization, self-bone grafting, the characteristic of bone conduction, can drop completely in human body
Solution, no rejection, there is good market prospect.
Specific embodiment
Below by embodiment, the present invention is described in further detail, but protection scope of the present invention be not limited to described
Content.
Embodiment 1: this is gone to organise the method that 3d printing prepares implantable bone graft using animal skeleton, particular content
As follows:
(1) take fresh Os Bovis seu Bubali to remove soft tissue, cleaned with normal saline, be placed at 1000 DEG C after carrying out calcining 4h, pulverize and be obtained
Particle diameter is less than 1mm osseous granuleses；
(2) polylactic acid is heated to 70 DEG C and is allowed to be formed pasty state, then mixed with osseous granuleses 1:3 ratio in mass ratio；
(3) carry out 3-D scanning by the preoperative patient is needed with implant part, draw the vertical of bone graft using mapping software
Rendering architecture is printed by body Model using 3d printer, and implantable bone graft is obtained after cooling.
Embodiment 2: this is gone to organise the method that 3d printing prepares implantable bone graft using animal skeleton, particular content
As follows:
(1) take fresh Os Caprae seu Ovis to remove soft tissue, cleaned with normal saline, be placed at 1100 DEG C after carrying out calcining 3h, pulverize and be obtained
Particle diameter is less than 1mm osseous granuleses；
(2) shitosan, Fibrin Glue are heated to 70 DEG C and are allowed to be formed pasty state, then binding agent (shitosan: Fibrin Glue
=mass ratio 1:1) mixed with osseous granuleses 1:5 ratio in mass ratio；
(3) carry out 3-D scanning by the preoperative patient is needed with implant part, draw the vertical of bone graft using mapping software
Rendering architecture is printed by body Model using 3d printer, and implantable bone graft is obtained after cooling.
Embodiment 3: this is gone to organise the method that 3d printing prepares implantable bone graft using animal skeleton, particular content
As follows:
(1) take fresh Os Sus domestica to remove soft tissue, cleaned with normal saline, be placed at 1000 DEG C after carrying out calcining 4h, pulverize and be obtained
Particle diameter is less than 1mm osseous granuleses；
(2) shitosan is heated to 60 DEG C and is allowed to be formed pasty state, then mixed with osseous granuleses 1:4 ratio in mass ratio；
(3) carry out 3-D scanning by the preoperative patient is needed with implant part, draw the vertical of bone graft using mapping software
Body Model, carries out wire drawing process to above-mentioned material, is formed long thread, after cooling, is printed rendering architecture using 3d printer,
Implantable bone graft is obtained after cooling.
After this product: 1, bone fusion probability can be improved further；2nd, by the growth of osseous tissue, this product can be completely
Be substituted for skeleton, solve the artificial bone-grafting material such as hydroxy phosphorus Calx in vivo can not replacement completely shortcoming；3rd, this product
Product are printed by 3d, can meet and be unable to the three-dimensional shape of position bone grafting in human body and require, make operation technique easier, shorten operation
Time, reduce operation risk；4th, it is printed as the three-dimensional shape needing by 3d, making of metallic in-laid thing in the patient can be reduced
With, the use of " titanium cage " inner matter in such as spinal operation, reduces the risk causing in metallic in-laid thing Preserving time body.
Claims (5)
1. a kind of the method for implantable bone graft is prepared it is characterised in that by such as using animal skeleton 3d printing of going to organise
Lower step is carried out:
(1) take fresh animal bone to remove soft tissue, cleaned with normal saline, be placed at 1000 DEG C -1100 DEG C and carry out calcining 3-4h
Afterwards, pulverize and osseous granuleses are obtained；
(2) binding agent is heated to 50 DEG C -70 DEG C and is allowed to be formed pasty state, then enter with osseous granuleses 1:3-1:5 ratio in mass ratio
Row mixing；
(3) utilize mapping software to draw the stereomodel of bone graft, be printed as painting by step (2) mixture using 3d printer
The stereomodel of system, obtains final product implantable bone graft after cooling.
2. according to claim 1 gone to organise the method that 3d printing prepares implantable graft using animal skeleton, its
It is characterised by: step (1) is pulverized and is less than 1mm for particle diameter.
3. according to claim 1 gone to organise the method that 3d printing prepares implantable graft using animal skeleton, its
It is characterised by: binding agent is shitosan, collagen, Fibrin Glue, a kind of or arbitrarily than several in polylactic acid.
4. according to claim 1 gone to organise the method that 3d printing prepares implantable graft using animal skeleton, its
It is characterised by: animal bone is Os Bovis seu Bubali, Os Caprae seu Ovis or Os Sus domestica.
5. according to claim 1 gone to organise the method that 3d printing prepares implantable graft using animal skeleton, its
It is characterised by: be prepared into long thread after binding agent and osseous granuleses mixing, be used further to 3d and print.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610737279.0A CN106344214A (en) | 2016-08-29 | 2016-08-29 | Method for preparing implantable bone grafts by de-organification and 3D printing of animal bones |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610737279.0A CN106344214A (en) | 2016-08-29 | 2016-08-29 | Method for preparing implantable bone grafts by de-organification and 3D printing of animal bones |
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| CN106344214A true CN106344214A (en) | 2017-01-25 |
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| CN201610737279.0A Pending CN106344214A (en) | 2016-08-29 | 2016-08-29 | Method for preparing implantable bone grafts by de-organification and 3D printing of animal bones |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107823712A (en) * | 2017-12-13 | 2018-03-23 | 华中科技大学 | A kind of method for preparing imitative artificial bone of coral with cuttlebone and products thereof |
| CN110025406A (en) * | 2019-04-17 | 2019-07-19 | 上海黑焰医疗科技有限公司 | A kind of preparation method of 3D printing bone defect filler |
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| CN101020082A (en) * | 2007-03-09 | 2007-08-22 | 高心 | Bone repairing material and its prepn process and use |
| CN101954122A (en) * | 2010-09-13 | 2011-01-26 | 陕西瑞盛生物科技有限公司 | Preparation method of natural bone repairing material with pre-plasticity |
| CN103284815A (en) * | 2013-05-17 | 2013-09-11 | 中山大学 | 3D-printing fast forming method of nano composite degradable bone repair material |
| WO2015003140A1 (en) * | 2013-07-03 | 2015-01-08 | Wayne State University | Method of making water soluble injectable calcium polyphosphate gels |
| CN104984414A (en) * | 2015-07-29 | 2015-10-21 | 陕西博与再生医学有限公司 | Composite absorbable interface screw and preparation method thereof |
-
2016
- 2016-08-29 CN CN201610737279.0A patent/CN106344214A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101020082A (en) * | 2007-03-09 | 2007-08-22 | 高心 | Bone repairing material and its prepn process and use |
| CN101954122A (en) * | 2010-09-13 | 2011-01-26 | 陕西瑞盛生物科技有限公司 | Preparation method of natural bone repairing material with pre-plasticity |
| CN103284815A (en) * | 2013-05-17 | 2013-09-11 | 中山大学 | 3D-printing fast forming method of nano composite degradable bone repair material |
| WO2015003140A1 (en) * | 2013-07-03 | 2015-01-08 | Wayne State University | Method of making water soluble injectable calcium polyphosphate gels |
| CN104984414A (en) * | 2015-07-29 | 2015-10-21 | 陕西博与再生医学有限公司 | Composite absorbable interface screw and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107823712A (en) * | 2017-12-13 | 2018-03-23 | 华中科技大学 | A kind of method for preparing imitative artificial bone of coral with cuttlebone and products thereof |
| CN110025406A (en) * | 2019-04-17 | 2019-07-19 | 上海黑焰医疗科技有限公司 | A kind of preparation method of 3D printing bone defect filler |
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Application publication date: 20170125 |
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