CN103599138B - Cordyceps militaris (L.) Link. extract lozenge - Google Patents
Cordyceps militaris (L.) Link. extract lozenge Download PDFInfo
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- CN103599138B CN103599138B CN201310592011.9A CN201310592011A CN103599138B CN 103599138 B CN103599138 B CN 103599138B CN 201310592011 A CN201310592011 A CN 201310592011A CN 103599138 B CN103599138 B CN 103599138B
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Abstract
The present invention relates to a kind of Cordyceps militaris (L.) Link. extract lozenge, comprise following component by weight percentage: cordycepin 5-30%, tapioca 0-30%, maltodextrin 0-50%, sucralose 0-2%, micropowder silica gel 0-1%.Cordyceps militaris (L.) Link. extract lozenge of the present invention is except cordycepin content significantly improves, and other effective ingredient are retained, and buccal tablet remains without hazardous solvent, green, healthy.Buccal tablet of the present invention introduces index---the cordycepin that can quantize to detect, and in the buccal tablet made, the cordycepin content of every a slice is fixed as certain value in 5%-30%.
Description
Technical field
The invention belongs to the field of deep of edible medicinal fungi, be specifically related to the buccal tablet that a kind of Cordyceps militaris (L.) Link. extract makes.
Background technology
The content of cordycepin in wild cordyceps is atomic, and the cordycepin of present institute generally all extracts from Cordyceps militaris (L.) Link., has antiviral, antitumor action.Cordycepin, as a kind of novel broad-spectrum antibiotic, with its distinctive antimicrobial antiviral activity, has caused the great attention of global scientific and technological developed country.It can suppress DNA and RNA of z tumor cell to synthesize; All inhibitory action is had to bacillus subtilis and mycobacterium tuberculosis avium; Also lethal effect is had to HIV-I type virus; Especially very strong inhibitory action is had to multiple solid malignant.Therefore, it can be used as a kind of broad-spectrum new drug to develop is inexorable trend.Existing cordyceps product and Cordyceps militaris (L.) Link. goods, mostly do not carry out refining processing, cannot remove the heavy metal in Cordyceps, topmost, and in these products, composition and content cannot accurate quantitative analysis, also do not seek unity of standard.
Summary of the invention
The object of this invention is to provide a kind of green, healthy, can the Cordyceps militaris (L.) Link. extract lozenge of quantification cordycepin content.
Cordyceps militaris (L.) Link. extract lozenge of the present invention, comprises following component by weight percentage: cordycepin 5-30%, tapioca 0-30%, maltodextrin 0-50%, sucralose 0-2%, micropowder silica gel 0-1%.
Described cordycepin takes from the dry cream of Cordyceps militaris (L.) Link. extract be made up of following method:
(1) Cordyceps militaris (L.) Link.sporophore is dry and pulverize;
(2) starting material with water after pulverizing is extracted, Extracting temperature 50-70 DEG C;
(3) extracting solution being concentrated to proportion at 50-70 DEG C is 1.1-1.5g/cm
3thick paste, add the ethanol of 2-5 times of thick paste volume, hold over night gets supernatant;
(4) diluted by supernatant and carry out purification with macroreticular resin, desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h;
(5) eluent is obtained the Cordyceps militaris (L.) Link. extract dry cream of cordycepin content at 15%-30% at 50-70 DEG C of concentrate drying.
Cordyceps militaris (L.) Link. extract lozenge of the present invention is the cordycepin product with physiologically active cordycepin, except cordycepin content significantly improves, other effective ingredient such as protein, aminoacid and trace element are also retained, and adopt purification with macroreticular resin to eliminate heavy metal in Cordyceps militaris (L.) Link. and harmful components simultaneously, buccal tablet remains without hazardous solvent, green, healthy.After cordycepin content improves, can to the health care of human body onset faster, for the treatment of tumour patient and the rehabilitation effect of some chronic diseases faster.Buccal tablet originality of the present invention is that first time introduces index---the cordycepin that can quantize to detect, and in the buccal tablet made, the cordycepin content of every a slice is fixed as certain value in 5%-30%, and can duplicate detection, takes relieved like this, rapid-action, taking convenience.
Accompanying drawing explanation
Accompanying drawing 1 is that the HPLC of the present invention's Cordyceps militaris (L.) Link. extract component used detects figure.
Detailed description of the invention
The present invention's dry cream of Cordyceps militaris (L.) Link. extract used is made up of following method:
Cordyceps militaris (L.) Link.sporophore (cordycepin content is at 0.1-0.5%) is dry, pulverize with 10-30 object screen cloth; By the raw material after pulverizing, take water as Extraction solvent, material-water ratio is 1:5-20, Extracting temperature 50-70 DEG C, and extraction time is 2-10h; Be 1.1-1.5g/cm by the extracting solution obtained with being concentrated to proportion
3thick paste, add the ethanol of 2-5 times of thick paste volume, hold over night is got supernatant and is carried out dilution process, and in order to carrying out purification with macroreticular resin, controlling load solution cordycepin mass concentration during upper prop is 0.6-2.0mg/mL, loading volume 2-10BV, loading flow velocity 1-3BV/h; Desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h, desorption solvent volume 4-10BV used; Eluent is concentrated into thick paste state, is then dried and obtains the Cordyceps militaris (L.) Link. extract dry cream of cordycepin content at 15%-30%.
In the embodiment of the present invention, the dry cream of cordycepin used is specifically made up of following method:
(1) pulverize: artificial cordyceps militaris fruiting body is dried to moisture below 5% at 50 DEG C, cross 20 eye mesh screens and pulverize;
(2) with water extraction: throw in the extraction pot of 3 tons by the Cordyceps militaris (L.) Link.sporophore that 150kg pulverizes, inject purified water 3000kg, solid-liquid ratio 1:20, and open stirring, temperature controls at 65-70 DEG C, and each soak extraction time is 10 hours, repeats soak extraction three times;
(3) concentrated precipitate with ethanol: by the extracting solution of three times mixing also, (proportion is 1.5g/cm to be evaporated to the thick paste of 80kg
3), wherein thickening temperature 50-70 DEG C, concentrated pressure-0.005 is to-0.009Mpa.In thick paste, add 95% ethanol of 5 times of thick paste volumes, it is for subsequent use that hold over night gets supernatant;
(4) macroporous adsorbent resin eluting: the content detecting cordycepin in supernatant after precipitate with ethanol, according to the volume of DM-130 resin, calculates the content of its absorption cordycepin; Then dilution process is carried out to supernatant, control load solution cordycepin mass concentration 0.2mg/mL, loading volume 10BV, loading flow velocity 3BV/h; Eluting solvent is 25% ethanol water, elution flow rate 2BV/h, elution volume 4BV.Eluent sampling detects with HPLC and quantitatively, wavelength 260nm, is shown in accompanying drawing 1.
(5) concentrate drying: by the eluent vacuum concentration eluted, is concentrated into thick paste under pressure is the-0.005Mpa state that extremely-0.009Mpa, temperature are 50-70 DEG C.Then proceeded to microwave drying oven, temperature and pressure is all the same with time concentrated, obtain after drying cordycepin content 15.6% the dry cream of Cordyceps militaris (L.) Link. extract (water content 5%).
Embodiment one
Get the dry cream 100g of Cordyceps militaris (L.) Link. extract of above-mentioned obtained cordycepin content 15.6%, add tapioca 80g, maltodextrin 100g, sucralose 2g, micropowder silica gel 2g, mix homogeneously, micronizing is to 20 μm, granulate, dry, namely tabletting obtains the Cordyceps militaris (L.) Link. extract lozenge that cordycepin content is 5.5% content.
Embodiment two
Get the dry cream 100g of Cordyceps militaris (L.) Link. extract of above-mentioned obtained cordycepin content 15.6%, add tapioca 50g, maltodextrin 150g, sucralose 6g, micropowder silica gel 3g, mix homogeneously, micronizing is to 20 μm, granulate, dry, namely tabletting obtains the Cordyceps militaris (L.) Link. extract lozenge that cordycepin content is 5% content.
Claims (1)
1. a Cordyceps militaris (L.) Link. extract lozenge, comprises following component by weight percentage: cordycepin 5-30%, tapioca 0-30%, maltodextrin 0-50%, sucralose 0-2%, micropowder silica gel 0-1%;
Described cordycepin takes from the dry cream of Cordyceps militaris (L.) Link. extract be made up of following method:
(1) Cordyceps militaris (L.) Link.sporophore is dry and pulverize;
(2) starting material with water after pulverizing is extracted, Extracting temperature 50-70 DEG C;
(3) extracting solution being concentrated to proportion at 50-70 DEG C is 1.1-1.5g/cm
3thick paste, add the ethanol of 2-5 times of thick paste volume, hold over night gets supernatant;
(4) diluted by supernatant and carry out purification with macroreticular resin, desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h;
(5) eluent is obtained the Cordyceps militaris (L.) Link. extract dry cream of cordycepin content at 15%-30% at 50-70 DEG C of concentrate drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310592011.9A CN103599138B (en) | 2013-11-22 | 2013-11-22 | Cordyceps militaris (L.) Link. extract lozenge |
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| CN201310592011.9A CN103599138B (en) | 2013-11-22 | 2013-11-22 | Cordyceps militaris (L.) Link. extract lozenge |
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| Publication Number | Publication Date |
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| CN103599138A CN103599138A (en) | 2014-02-26 |
| CN103599138B true CN103599138B (en) | 2015-12-09 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585733B (en) * | 2014-12-31 | 2016-08-24 | 台州康晶生物科技有限公司 | Cordyceps oral tablet and preparation method thereof |
| CN105361173A (en) * | 2015-12-14 | 2016-03-02 | 梁文成 | Bighead atractylodes rhizome cordyceps sinensis buccal tablets and making method thereof |
| CN106465932A (en) * | 2016-03-15 | 2017-03-01 | 曾建平 | The compound method of Cordycepses Fructus Lycii buccal tablet |
| CN105902577A (en) * | 2016-04-10 | 2016-08-31 | 台州康晶生物科技有限公司 | Low-sugar Cordyceps militaris oral tablet and preparation method thereof |
| CN108606311A (en) * | 2017-02-08 | 2018-10-02 | 王润蛟 | High cordycepin Cordyceps sinensis buccal tablets production new technology |
| CN107114779A (en) * | 2017-05-26 | 2017-09-01 | 吉林省华惠生物科技有限公司 | The chewable tablets and preparation method of the extract of Cordyceps militaris containing ginseng stem and leave general saponin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294133A (en) * | 2000-11-06 | 2001-05-09 | 吉林东北虎药业股份有限公司 | Process for extracting cordycepin and its preparation |
| CN101928316A (en) * | 2010-07-23 | 2010-12-29 | 上海国宝企业发展中心 | Preparation method of high-purity plate-like cordycepin crystal |
| CN103071015A (en) * | 2012-12-11 | 2013-05-01 | 上海浦东高星生物技术研究所 | Compound Cistanche deserticola tablet |
| CN103156025A (en) * | 2011-12-15 | 2013-06-19 | 王润蛟 | Production technology of cordyceps sinensis nanometer powder capsule eating tea with high cordycepin content |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432656B (en) * | 2011-12-23 | 2014-07-02 | 辽宁省农业科学院大连生物技术研究所 | Method for extracting and purifying cordycepin from Cordyceps militaris sporophore |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294133A (en) * | 2000-11-06 | 2001-05-09 | 吉林东北虎药业股份有限公司 | Process for extracting cordycepin and its preparation |
| CN101928316A (en) * | 2010-07-23 | 2010-12-29 | 上海国宝企业发展中心 | Preparation method of high-purity plate-like cordycepin crystal |
| CN103156025A (en) * | 2011-12-15 | 2013-06-19 | 王润蛟 | Production technology of cordyceps sinensis nanometer powder capsule eating tea with high cordycepin content |
| CN103071015A (en) * | 2012-12-11 | 2013-05-01 | 上海浦东高星生物技术研究所 | Compound Cistanche deserticola tablet |
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Effective date of registration: 20170113 Address after: Tairan Industrial Zone 518000 Guangdong city of Shenzhen province Futian District Che Kung Temple Industrial Zone 503 building seventh layer 704 (office only) Patentee after: Shenzhen Dacheng grass Biotechnology Co., Ltd. Address before: 315800 Mount Lu, Ningbo, Zhejiang, South China 167-4 Patentee before: NINGBO GUANGFA WENBO BIO-SCIENCE TECHNOLOGY CO., LTD. |
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