CN103599138A - Cordyceps militaris extract lozenge - Google Patents

Abstract

The invention relates to a cordyceps militaris extract lozenge, comprising the following components by weight percent: 5-30% of cordycepin, 0-30% of cassava starch, 0-50% of maltodextrin, 0-20% of sucralose and 0-1% of superfine silica powder. By adopting the cordyceps militaris extract lozenge provided by the invention, the other effective components are reserved, the cordycepin content is also greatly improved; the lozenge is free of harmful solvent residue, green and healthy. An index, namely the cordycepin, which can be quantitatively detected, is led to the lozenge disclosed by the invention; the cordycepin content in each prepared lozenge is fixed at a fixed value between 5% and 30%.

Description

Cordyceps militaris (L.) Link. extract lozenge

Technical field

The invention belongs to the deep processing field of edible medicinal fungi, be specifically related to a kind of buccal tablet of making of Cordyceps militaris (L.) Link. extract.

Background technology

The content of cordycepin in wild cordyceps is atomic, and the cordycepin that institute is used now generally all extracts from Cordyceps militaris (L.) Link., has antiviral, antitumor action.Cordycepin, as a kind of novel broad-spectrum antibiotic, with its distinctive antimicrobial antiviral activity, has caused the great attention of global scientific and technological developed country.DNA and RNA that it can suppress z tumor cell synthesize; Bacillus subtilis and mycobacterium tuberculosis avium are all had to inhibitory action; HIV-I type virus is also had to lethal effect; Especially multiple solid malignant is had to very strong inhibitory action.Therefore using it, as a kind of broad-spectrum new drug exploitation, be, inexorable trend.Existing cordyceps product and Cordyceps militaris (L.) Link. goods, do not carry out refining processing mostly, cannot remove the heavy metal in Cordyceps, topmost, and in these products, composition and content cannot accurate quantitative analysis, unified standard not yet.

Summary of the invention

The object of this invention is to provide a kind of green, health, Cordyceps militaris (L.) Link. extract lozenge that can quantification cordycepin content.

Cordyceps militaris (L.) Link. extract lozenge of the present invention, comprises following component by weight percentage: cordycepin 5-30%, tapioca 0-30%, maltodextrin 0-50%, sucralose 0-2%, micropowder silica gel 0-1%.

Described cordycepin is taken from the dry cream of Cordyceps militaris (L.) Link. extract of being made by following method:

(1) Cordyceps militaris (L.) Link.sporophore is dried and is pulverized;

(2) by the raw material water extraction after pulverizing, extract temperature 50-70 ℃;

(3) extracting solution being concentrated to proportion at 50-70 ℃ is 1.1-1.5g/cm ³thick paste, add the ethanol of 2-5 times of thick paste volume, standing over night is got supernatant;

(4) supernatant diluted and carry out purification with macroreticular resin, desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h;

(5) eluent is obtained to cordycepin content at the dry cream of Cordyceps militaris (L.) Link. extract of 15%-30% at 50-70 ℃ of concentrate drying.

Cordyceps militaris (L.) Link. extract lozenge of the present invention is the cordycepin product with physiologically active cordycepin, except cordycepin content significantly improves, other effective ingredient are also retained as protein, aminoacid and trace element, and adopt purification with macroreticular resin to remove heavy metal and the harmful components in Cordyceps militaris (L.) Link. simultaneously, buccal tablet is residual without hazardous solvent, green, healthy.After cordycepin content improves, can be to the health care of human body onset faster, faster for the treatment of tumour patient and the rehabilitation effect of some chronic diseases.Buccal tablet originality of the present invention has been to introduce for the first time and can have quantized index---the cordycepin that detects, and in the buccal tablet of making, the cordycepin content of every a slice is fixed as certain value in 5%-30%, and can duplicate detection, take so relieved, rapid-action, taking convenience.

Accompanying drawing explanation

The HPLC that accompanying drawing 1 is the present invention's Cordyceps militaris (L.) Link. extract component used detects and schemes.

The specific embodiment

The present invention's dry cream of Cordyceps militaris (L.) Link. extract used is made by following method:

Cordyceps militaris (L.) Link.sporophore (cordycepin content is at 0.1-0.5%) is dry, with 10-30 object screen cloth, pulverize; By the raw material after pulverizing, take water as extracting solvent, material-water ratio is 1:5-20, extracts temperature 50-70 ℃, extraction time is 2-10h; By the extracting solution obtaining, with being concentrated to proportion, be 1.1-1.5g/cm ³thick paste, the ethanol that adds 2-5 times of thick paste volume, standing over night is got supernatant and is carried out dilution process, and in order to carrying out purification with macroreticular resin, during upper prop, controlling loading solution cordycepin mass concentration is 0.6-2.0 mg/mL, loading volume 2-10BV, loading flow velocity 1-3BV/h; Desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h, desorption solvent volume 4-10BV used; Eluent is concentrated into thick paste state, is then dried and obtains cordycepin content at the dry cream of Cordyceps militaris (L.) Link. extract of 15%-30%.

In the embodiment of the present invention, the dry cream of cordycepin used is specifically made by following method:

(1) pulverize: artificial cordyceps militaris fruiting body is dried to moisture below 5% at 50 ℃, crosses 20 eye mesh screens and pulverize;

(2) with water extraction: the Cordyceps militaris (L.) Link.sporophore that 150kg is pulverized is thrown in the extraction pot of 3 tons, injects purified water 3000kg, solid-liquid ratio 1:20, and open and stir, temperature is controlled at 65-70 ℃, and soak extraction time is 10 hours at every turn, repeats to soak and extracts three times;

(3) concentrated precipitate with ethanol: the extracting solution of three times is mixed also, and (proportion is 1.5g/cm to be evaporated to the thick paste of 80kg ³), thickening temperature 50-70 ℃ wherein, concentrated pressure-0.005 is to-0.009Mpa.95% ethanol that adds 5 times of thick paste volumes in thick paste, it is standby that standing over night is got supernatant;

(4) macroporous adsorbent resin eluting: the content of cordycepin in supernatant after detection precipitate with ethanol, according to the volume of DM-130 resin, calculate the content of its absorption cordycepin; Then supernatant is carried out to dilution process, control loading solution cordycepin mass concentration 0.2 mg/mL, loading volume 10BV, loading flow velocity 3BV/h; Eluting solvent is 25% ethanol water, elution flow rate 2BV/h, elution volume 4BV.Eluent sampling detects with HPLC and quantitatively, wavelength 260nm, is shown in accompanying drawing 1.

(5) concentrate drying: by the eluent vacuum concentration eluting, be to be concentrated into thick paste under the state of 50-70 ℃ to-0.009Mpa, temperature for-0.005Mpa at pressure.Then proceeded to microwave drying oven, temperature and pressure is all the same when concentrated, obtains cordycepin content after dry at 15.6% the dry cream of Cordyceps militaris (L.) Link. extract (water content 5%).

Embodiment mono-

Get the dry cream 100g of Cordyceps militaris (L.) Link. extract of the above-mentioned cordycepin content making 15.6%, add tapioca 80g, maltodextrin 100g, sucralose 2g, micropowder silica gel 2g, mix homogeneously, micronizing is to 20 μ m, granulate, dry, tabletting obtains the Cordyceps militaris (L.) Link. extract lozenge that cordycepin content is 5.5% content.

Embodiment bis-

Get the dry cream 100g of Cordyceps militaris (L.) Link. extract of the above-mentioned cordycepin content making 15.6%, add tapioca 50g, maltodextrin 150g, sucralose 6g, micropowder silica gel 3g, mix homogeneously, micronizing is to 20 μ m, granulate, dry, tabletting obtains the Cordyceps militaris (L.) Link. extract lozenge that cordycepin content is 5% content.

Claims (2)

1. a Cordyceps militaris (L.) Link. extract lozenge, comprises following component by weight percentage: cordycepin 5-30%, tapioca 0-30%, maltodextrin 0-50%, sucralose 0-2%, micropowder silica gel 0-1%.

2. Cordyceps militaris (L.) Link. extract lozenge according to claim 1, is characterized in that described cordycepin takes from the dry cream of Cordyceps militaris (L.) Link. extract of being made by following method:

(1) Cordyceps militaris (L.) Link.sporophore is dried and is pulverized;

(2) by the raw material water extraction after pulverizing, extract temperature 50-70 ℃;

(3) extracting solution being concentrated to proportion at 50-70 ℃ is 1.1-1.5g/cm ³thick paste, add the ethanol of 2-5 times of thick paste volume, standing over night is got supernatant;

(4) supernatant diluted and carry out purification with macroreticular resin, desorption solvent is 20-50% ethanol water, elution flow rate 2-5BV/h;

(5) eluent is obtained to cordycepin content at the dry cream of Cordyceps militaris (L.) Link. extract of 15%-30% at 50-70 ℃ of concentrate drying.

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