CN103598203B - Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech - Google Patents
Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech Download PDFInfo
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- CN103598203B CN103598203B CN201310560782.XA CN201310560782A CN103598203B CN 103598203 B CN103598203 B CN 103598203B CN 201310560782 A CN201310560782 A CN 201310560782A CN 103598203 B CN103598203 B CN 103598203B
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Abstract
The invention discloses a selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech. According to the technical scheme provided by the invention, the selective compound pesticide comprises 2% of sophocarpidine, 25% of exorista sorbillans destorying emulsion and water in a mass ratio of 1 to (1.8-2.2) to (1800-2200); the selective compound pesticide is sprayed on leaf surfaces of mulberries according to a dosage of 45-55 kilograms on each 667 square meters and has no toxin to people and livestock; the safety interval to domestic silkworms is 8 hours. The selective compound pesticide is simple to prepare and low in price, is made from the easily available materials, has low toxin, low residue and no pollution, is convenient to use and can be directly sprayed on the leaf surfaces of the mulberries. A test shows that the control rate is up to about 80%; the control rate of other pests including phthonandria atrilineata butler, ascotis selenaria, spilarctia subcarnea walker, euproctis similis fuessly, acronicta major bremer and the like is maintained to be about 90%; the selective compound pesticide is popularized and applied in about one hectare of mulberry field in Teyong Town of Sheyang County of Yancheng City and about one hectare of mulberry field of Xinfeng Town of Dafeng City, and obtains satisfied control effects.
Description
Technical field
Patent of the present invention relates to a kind of selectivity Recompounded pesticide preventing and treating two kinds of common insect pests diaphanias and Bombyx mandarina in mulberry field.
Background technology
Along with the use of high-toxic pesticide and the change of environmental condition, the pesticide resistance of most of Mulberry pest there occurs change, traditional prevention and controls of employing, has been difficult to effectively to control it and has caused harm.Constantly saw the report having all parts of the country " Mulberry pest outburst is caused harm, and is eaten up by mulberry leaf rear without mulberry leaf hello silkworm " in recent years.Vehicles Collected from Market can be bought, and for preventing and treating the conventional chemical pesticide of Mulberry pest, the enemy enemy as 80% feeds 1500 times, the phoxim 1500 times etc. of 50%, and drug effect is single, only has the control efficiency of about 30 ~ 60%.In addition to the high poison of people and animals, high residue, particularly summer the mulberry field height of tree, sky heat, stuffy, careless slightly, very easily there is people and animals and silkworm intoxication accident.Cai state auspicious grade (2011) paper " botanical pesticide matrine is to the virulence of part Mulberry pest and field control effect " and Chinese patent CN102523995A disclose from the novel pesticide producing popularization for this reason, what filter out is short to silkworm Residual time, to mulberry tussock moth, mulberry geometrid (Hemerophila atrilineata), cotton large bridging worm, herba aristolochiae millissimae, white tiger moth, Phellinus linteus, Tetranychus cinnabarinus has this selective pesticide of botanical pesticide matrine of good control efficiency, but due to this medicament to diaphania and these two kinds of important pests of wild silkworm insensitive, cause control efficiency poor, thus limit matrine agricultural chemicals Mulberry pest prevent and treat using of aspect and promote.
Summary of the invention
For overcoming the deficiencies in the prior art, inventor by laboratory test, outdoor investigation, filters out the optimization of C/C composites of the special selectivity Recompounded pesticide in mulberry field, improves control efficiency, expand controlling object scope, reduce cost accounting in basis of background technology.
The technical solution adopted for the present invention to solve the technical problems is:
The matrine of 2%, the silkworms tachinia files eardication of 25%, water, the proportioning (mass ratio) of three is: 1: 1.8 ~ 2.2: 1800 ~ 2200; With the consumption of every 667 square metres 45 ~ 55 kilograms, spray on the blade face of mulberry tree, nontoxic to people and animals, be 8 hours to the safety interval of silkworm.Its preparation of the present invention is simple, raw material sources wide, cheap, low toxicity, low-residual, pollution-free, easy to use, can directly spray on the blade face of mulberry tree.The results showed, its control efficiency reaches about 80%, to other insect as the control efficiency such as mulberry geometrid (Hemerophila atrilineata), cotton large bridging worm, white tiger moth, mulberry tussock moth, herba aristolochiae millissimae are stabilized in about 90%, apply in each about 1 hectare mulberry field, Xinfeng Town of Te Yong town, Yancheng City Sheyang County and Dafeng City, all achieve satisfied control efficiency.
The medicament related in the present invention and biomaterial are:
For examination agricultural chemicals 2% aqueous matrine solution, for Tianjin Hengyuan Biological Technology Development Co., Ltd.'s product, the interim registration card number of agricultural chemicals is LS20090197, production licence number is HNP12058-K0497, product standard number is Q/12NY 0821-2007, the registration crop of test sample agricultural chemicals is brassicaceous vegetable, and registration controlling object is cabbage caterpillar.For examination agricultural chemicals 25% silkworm fly repellant oil, for Tou Zaocan pharmaceutical factory, Dongtai City produces, authentication code: veterinary drug word (2007) 100749827, contrast agricultural chemicals 50% phoxim emulsifiable concentrate, for Qingdao Hailir Pesticides and Chemical Co., Ltd. of Shandong Province product, card number stepped on by agricultural chemicals: PD20098424.
For examination mulberry tree breed for educating 7-11, life in 6 ~ 7 years.For examination Mulberry Pests diaphania (Diaphaniapyloalis Walker), Bombyx mandarina (Bombyx mandarina Leech), mulberry geometrid (Hemerophila atrilineata) (Phthonandria atrilineata Butler), mulberry tussock moth (Euproctis similes Fuessly), cotton large bridging worm (Ascotis selenaria Schiff.et Denis), white tiger moth (Spilarctia subcarnea Walker), the examination insect that supplies of herba aristolochiae millissimae (Acronicta major Bremer) and different developmental phases thereof all gathers from mulberry field, northeast part of China village, Te Yong town, Sheyang County of Jiangsu Province, the insect wherein carrying out agricultural chemicals toxicity test gathers chalaza all outdoor in mulberry field and returns indoors artificial and raise 4 instar larvaes for examination.Examination cultivated silkworm breed variety is supplied to be SuJu×MingHu.
The test method that the present invention adopts is
4 ages more than are got for each 15 ~ 20 of examination insect diaphania in the 1 each experimental plot of Recompounded pesticide recipe determination laboratory test, artificial feeding is covered with mulberry leaf, is lined with in the double ware (diameter 12cm) of blotting paper below above, by experimental scheme, the dilute liquid medicine of preparation different agents, variable concentrations, evenly be sprayed on mulberry leaf by hand-held atomizer, to squirt blade for degree, the Mortality of insect of each experimental plot of 24h " Invest, Then Investigate ".Wherein, insect with guttation, be motionlessly designated as death; Normally creep and be designated as survival; Dizzy fan, be slow in action and charge to death by 0.5.Often kind of insect small plot experiment repeats for 3 times.Commonly use medicament 50% phoxim 2000 times of dilutions with mulberry field to compare, separately establish blank.
2 medicaments will establish 5 the concentration gradient process of 4000,2000,1000,500,250 times of dilutions for examination 2% matrine to the Toxicity Determination of insect, the silkworms tachinia files eardication of 25% establishes 5 the concentration gradient process of 2000,1000,500,250,125 times of dilutions, Recompounded pesticide is composite rear by 5 the concentration gradient process of 4000,2000,1000,500,250 times of dilutions with the silkworms tachinia files eardication 1: 2 of 2% matrine and 25%, according to the lethality of diaphania and Bombyx mandarina two kinds of insects after the method investigation 24h of " 1 ", calculate virulence regression equation, the lethal concentration of 50 (LC
50), 95% confidence interval and co-toxicity coefficient (CTC), analyze different agents to the virulence for examination insect.Data analysis is undertaken by the method recorded in textbook " chemical protection of plant ".
3 Recompounded pesticides are investigated the safety testing Residual time of silkworm: Recompounded pesticide by 2% matrine and 25% silkworms tachinia files eardication 1: 2 composite after, mulberry tree is sprayed respectively with the dilution of 2 000,1 500,1 000 times, 1 mulberry leaf feeding 3 ~ 4 silkworm in age is adopted respectively every 4h, observe the reaction of silkworm, record active situation, until silkworm normally takes food.On the physical signs of silkworm and the impact investigation of economic indicator: personal distance after date is normally plucked mulberry leaf and fed silkworm, (each household investigates 30 silkworms to the sericulture achievement of random inspection 3 test peasant household, average), compare to use 2000 times of 50% phoxim dilution, separately establish blank, survey item comprised for 4 ages through (h), 4 hibernating worms weight (g), 5 ages are through (h), matured silkworm body weight (g), cocoon weight (g), cocoon shell weight (g), Cocoon layer ratio (%) etc., wherein developmental duration passes through as benchmark with more than 95% astogeny.
The mulberry geometrid (Hemerophila atrilineata) (Phthonandria atrilineata Butler) that 4 Recompounded pesticides will collect in outdoor mulberry field the control efficiency laboratory test of other Mulberry Pests, mulberry tussock moth (Euproctis similes Fuessly), cotton large bridging worm (Ascotis selenaria Schiff.et Denis), white tiger moth (Spilarctia subcarnea Walker), the larva of herba aristolochiae millissimae (Acronicta major Bremer) etc. is manually watched to support and is covered with mulberry leaf above, be lined with in the double ware (diameter 12cm) of blotting paper below, again by agricultural chemicals by 2% matrine and 25% silkworms tachinia files eardication 1: 2 composite after be diluted to 2000 times of solution, mulberry leaf are squirted with manual sprayer, the Mortality of insect of each experimental plot of 24h " Invest, Then Investigate ", same chemicals treatment, repeating for three times. the standard that calculates is with reference to the same.
The field controling test test of 5 insects is carried out in by the end of September, 2012 in, and test site is located at Te Yong town, Yancheng City Sheyang County of Jiangsu Province and Xinfeng Town, Dafeng City, and the mulberry field area of each point is 1 ~ 2hm
2(about 5 ~ 10 peasant households).Before test and after dispenser 24h, each test site random inspection 3 peasant households, 5, the mulberry field sampling of each peasant household, every sampling point investigates 15 strain mulberry treies, records the insect population number of various insect, and calculating insect population decreases rate.Two test sites all stay 1 ~ 2 family not execute any medicament, and spray clear water compares.For the investigation method of worm amount before and after the test of Phellinus linteus and Tetranychus cinnabarinus 2 kinds of microbody insects be: each sampling point every strain mulberry tree random inspection branch, record the worm amount of this root branch 5th ~ 6 proper mature (ripe) leaf backs from top to down.Relate to the survey data of percentage, all through arcsine conversion (Sin
-1√ X) after statistical analysis again.Data analysing method is undertaken by the method for textbook " agriculture Mathematical Statistics Analysis " this bibliography.
The relevant calculating formulas related in specification:
Insect population to decrease before rate=(before control after average worm amount-control average worm amount)/control on average worm amount × 100%;
Lethality=dead borer population/investigate total borer population × 100%.
Selectivity Recompounded pesticide recipe determination result of the present invention is as table 1, and wherein No. 12 process is routine administration, is Routine control district, and No. 13 is treated to clear water contrast, and No. 10, No. 11 is single dose treatment region.Can find out that No. 4 treatment region Mortality of insect is significantly higher than other treatment region from investigation result, medicament effective ingredient be 2% matrine, 25% silkworms tachinia files eardication, water, the proportioning of three is: 1: 2: 2000.
Table 1: selectivity Recompounded pesticide formulation screening test
Note: A is 2% matrine, and B is 25% silkworms tachinia files eardication, and C is water, and D is Routine control medicament 50% phoxim, and " * " is remarkable with conventional dose contrast difference, and " * * " is extremely remarkable with conventional dose contrast difference, and following form and table are noted same.
Selectivity Recompounded pesticide of the present invention is to diaphania and Bombyx mandarina two kinds of insect toxicity test results as table 2, and co-toxicity coefficient is respectively 477.0 and 479.6, and by the criterion of textbook " chemical protection of plant ", synergistic effect is obvious.
Table 2: selectivity Recompounded pesticide is to the toxicity test of diaphania and Bombyx mandarina two kinds of insects
4 instar larvaes are for examination insect.
Selectivity Recompounded pesticide of the present invention measures determination data as table 3 to the safety interval of silkworm, from table 3, record result find out, 2000 times of dilution safety interval only 8h of Recompounded pesticide, 1500 times of dilution safety intervals are 16h, 1000 times of dilution safety intervals are 32h, even if the large generation of sericulture production period insect, if with 2000 times of dilution pest controls, substantially do not affect production.
Table 3: selectivity Recompounded pesticide measures the safety interval of silkworm
For examination silkworm be 4 age glutonous stage larva, grey shading illustrates these stage food refusal mulberry leaf.
Selectivity Recompounded pesticide of the present invention on the impact of silkworm major economic indicators and physical signs as table 4, record result from table 4,5 ages of silkworm, matured silkworm body weight significantly increased through significant prolongation, cocoon weight and cocoon shell weight also obviously increase, but 4 ages of Cocoon layer ratio and silkworm are not obvious through increasing.Illustrate that selectivity Recompounded pesticide is safe to silkworm.
Table 4: family nibbles lower selectivity Recompounded pesticide process mulberry leaf to growing and the impact of amount of cocoon crop
Selectivity Recompounded pesticide of the present invention, is found out from table 5 data in table 5 control efficiency of other Mulberry Pests, selectivity Recompounded pesticide to the control efficiency of diaphania and wild silkworm two kinds of insects far above the matrine of single dose 2%, the silkworms tachinia files eardication of 25%.But it is close to the single matrine of the control efficiency such as other insect mulberry geometrid (Hemerophila atrilineata), cotton large bridging worm, white tiger moth, mulberry tussock moth, herba aristolochiae millissimae and 2%.
Selectivity Recompounded pesticide field experiment result of the present invention is as table 6, as can be seen from Table 6, the control efficiency of selectivity Recompounded pesticide to diaphania and wild silkworm two kinds of insects is stabilized in about 80%, about 90% is stabilized in control efficiency such as other insect mulberry geometrid (Hemerophila atrilineata), cotton large bridging worm, white tiger moth, mulberry tussock moth, herba aristolochiae millissimaes, this and laboratory test results are substantially identical, although can think the slightly biased height of Recompounded pesticide cost accounting, but due to people and animals and silkworm low toxicity, in production, spread is feasible.
Table 5: selectivity Recompounded pesticide records table (24h lethality-%) to other pest controling effect indoor experimental data of mulberry field
Table 6 selectivity Recompounded pesticide is to the field control effect of several Mulberry Pests (insect population decrease rate/-%)
Te Yong point medication area is 1.05 hectares, and Xinfeng point medication area is 0.92 hectare, and clear water contrast is special mediocre, test site, Xinfeng two area is 0.25 hectare.
The invention provides a mulberry field selectivity Recompounded pesticide formula, its effective ingredient be 2% matrine, 25% silkworms tachinia files eardication, water, the proportioning of three is: 1: 2: 2000, is 8 hours, to diaphania and Bombyx mandarina LC through investigation to the safety interval of silkworm
50be respectively: 0.360 and 0.400, its co-toxicity coefficient is respectively 477.0 and 479.6, and synergistic effect is obvious, can reach 80%, also have good control efficiency to other insect of mulberry field to the control efficiency of diaphania and wild silkworm.Through rural area, small size is promoted the use of, Sericultural production is had no adverse effects, harmless to person poultry safety, environmentally safe, meet the industrial policy of country's " low-carbon (LC), environmental protection, ecology ", this optimizes mulberry field, rural area Mulberry pest control techniques to innovation, alleviates and produces control of insect in season and the contradiction of producing of breeding silkworms, have important realistic meaning.
Embodiment
Embodiment one:
Get the matrine 6ml of commercial 2%, 25% silkworms tachinia files eardication 12ml makes inner wrapping injection, as the application dosage of 167 square metres of Mulberry Pests controls, for for subsequent use.During use, converting pH value is the neutral water of 7.0 ~ 7.6 or the water 12KG of neutral slightly biased alkali, now with the current, directly sprays the blade face in mulberry tree.Safe picking leaves phase of spraying is 8 hours.
Claims (2)
1. the selectivity Recompounded pesticide for preventing and treating for diaphania and Bombyx mandarina two kinds of Mulberry pest, it is characterized in that: comprise matrine, silkworms tachinia files eardication and water, the proportioning (mass ratio) of the matrine of 2%, the silkworms tachinia files eardication of 25% and water three is 1:1.8 ~ 2.2:1800 ~ 2200, with the dosage of every 667 square metres 45 ~ 55 kilograms, spray on the blade face of mulberry tree, nontoxic to people and animals, be 8 hours to the safety interval of silkworm.
2. according to claim 1 a kind of for the selectivity Recompounded pesticide for diaphania and Bombyx mandarina two kinds of Mulberry pest controls, it is characterized in that: described water is water that is neutral or slightly biased alkalescence, and pH value is 7.0 ~ 7.6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310560782.XA CN103598203B (en) | 2013-11-04 | 2013-11-04 | Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech |
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| CN201310560782.XA CN103598203B (en) | 2013-11-04 | 2013-11-04 | Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech |
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| CN103598203B true CN103598203B (en) | 2015-04-29 |
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| CN104926554A (en) * | 2015-06-05 | 2015-09-23 | 柳州环山科技有限公司 | Pesticide spray solution for white grub pest control of fructus mori |
| CN105767021B (en) * | 2016-04-07 | 2018-10-16 | 河南城建学院 | Kill people's line dirt moths attracted by lamplight larva extracting solution and its preparation method and application |
| CN113796387B (en) * | 2021-09-27 | 2022-09-20 | 陆琳 | Method for preventing and treating myiasis of silkworms |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008091650A1 (en) * | 2007-01-23 | 2008-07-31 | Marrone Organic Innovations, Inc. | Pesticidal formulation containing oxymatrine or matrine |
| CN102523995A (en) * | 2010-12-28 | 2012-07-04 | 蔡国祥 | New method for controlling mulberry pests |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008091650A1 (en) * | 2007-01-23 | 2008-07-31 | Marrone Organic Innovations, Inc. | Pesticidal formulation containing oxymatrine or matrine |
| CN102523995A (en) * | 2010-12-28 | 2012-07-04 | 蔡国祥 | New method for controlling mulberry pests |
Non-Patent Citations (2)
| Title |
|---|
| 植物源农药苦参碱对部分桑园害虫的毒力及田间防治效果;蔡国祥 等;《蚕业科学》;20111231;第37卷(第3期);第538-543页 * |
| 陈继久.桑树病虫害的综合防治.《四川蚕业》.2006,(第1期),第21-23页. * |
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