CN103598095A - Rapid seedling growing method for phlomis younghusbandii - Google Patents
Rapid seedling growing method for phlomis younghusbandii Download PDFInfo
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- CN103598095A CN103598095A CN201310563342.XA CN201310563342A CN103598095A CN 103598095 A CN103598095 A CN 103598095A CN 201310563342 A CN201310563342 A CN 201310563342A CN 103598095 A CN103598095 A CN 103598095A
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Abstract
The invention discloses a rapid seedling growing method for phlomis younghusbandii. The rapid seedling growing method comprises the following steps: selecting full phlomis younghusbandii seeds, immersing the phlomis younghusbandii seeds in 1000 times of 75% chlorothalonil diluting solution for 30 minutes, then immersing in clean water for 24 hours, and finally, washing by clean water for future use; putting the immersed phlomis younghusbandii seeds on a tray on which a wet non-woven fabric is laid in advance, putting the tray into a constant temperature box at 25-35 DEG C for culturing the seeds for 48 hours, and adding water into the tray to keep wet; preparing a culture medium from sediments, sheep manure and halite in a volume ratio of 5 to 3 to 2 and laying the culture medium into small holes of a hole disc; after carrying out the constant temperature culture for 48 hours, dibbling germinated seeds into the seedling growing hole disc which is fully filled with the medium, and covering the surface of the hole disc with one layer of thin soil after the seeds are sowed; and putting the hole disc into a seedling growing greenhouse for culturing the seeds after the dibbling is finished, supplementing water into the hole disc during the culturing period and transplanting seedlings with soil after the seeds grow into the seedlings after 25-30 days. According to the rapid seedling growing method for the phlomis younghusbandii, the survival rate of germchit is improved, the seedling growing process is simple, the seedling growing cost is low and the seedling growing period is short.
Description
Technical field
The invention belongs to younghusband jerusalemsage root seedling growing process field, relate in particular to a kind of fast seedling-cultivating method of younghusband jerusalemsage root.
Background technology
Younghusband jerusalemsage root is Labiatae Paraphlomis herbaceos perennial, is conventional Tibetan medicine.The develop rapidly of biotechnology in recent years, has driven the development of medical biotechnology industry.It is modern state-of-the-art plant propagation technology that tissue is cultivated, and at present, the mode that younghusband jerusalemsage root breeding is mainly cultivated by tissue is bred.
Younghusband jerusalemsage root is organized incubation step:
1, set up sterile system: the younghusband jerusalemsage root seed of selecting full seed, after alcohol with 70% is processed, with 0.1% mercuric chloride, carry out surface sterilization again, remove thimerosal, aseptic water washing 5-6 time, be seeded in MS medium and be placed in the 12h photoperiod, in the illumination cultivation chamber that 1500LX light intensity is lower 23/14 ℃, cultivate, through 25-30d aseptic seedling, grow up to.
2, the propagation of younghusband jerusalemsage root aseptic seedling is cultivated: for screening best proliferated culture medium, the younghusband jerusalemsage root aseptic seedling after seed sprouting is inoculated in the MS medium of additional variable concentrations to statistics propagation multiple after 30d.
3, the culture of rootage of younghusband jerusalemsage root test-tube plantlet: aseptic seedling is inoculated into and takes root induction period in the MS medium that adds variable concentrations NAA, observe its situation of taking root after 30d.
The shortcoming of prior art:
1, prior art exists test tube survival rate low;
2, prior art program is complicated, and operation easier is larger, and large batch of production operability is not strong;
3, prior art is cultivated for tissue, and equipment and instrument is had relatively high expectations;
4, prior art growing-seedling period is longer, needs more than February from aseptic seedling Establishing to last hardening;
5, prior art, because equipment requirement is high, growing-seedling period is long, operation easier is large, program is complicated, low its cost that causes of survival rate of seedling is higher, is therefore not suitable for large-scale production.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of fast seedling-cultivating method of younghusband jerusalemsage root, is intended to solve that existing younghusband jerusalemsage root grows seedlings that the survival rate that exists is low, complicated operation, long problem of cycle.
The embodiment of the present invention is achieved in that a kind of fast seedling-cultivating method of younghusband jerusalemsage root, and the fast seedling-cultivating method of this younghusband jerusalemsage root comprises the following steps:
Step 1, soaks seed, and chooses the younghusband jerusalemsage root seed of full seed, soaks 30 minutes, then soak 24 hours with clear water with 1000 times of 75% tpn dilution, rinses once standby more afterwards with clear water;
Step 2, insulating box vernalization, is placed in soaked younghusband jerusalemsage root seed in the insulating box that is put into again 25 ℃～35 ℃ in the pallet of completing in advance moistening nonwoven, cultivates 48 hours, to pallet, adds water to keep moistening;
Step 3, the configuration of seedling culture hole plate matrix, by silt, sheep excrement, pearl salt volume ratio, according to 5:3:2 proportional arrangement medium, silt sheep excrement pearl salt is placed in cubing container and according to the volume ratio of volume ratio 5:3:2 and is weighed, after they being stirred with instrument, fully mix, finally paving fills in the dish aperture of cave again;
Step 4, the seed program request of sprouting, will be after 48 hours constant temperature culture, and the seed program request of sprouting is to filling up in the seedling culture hole plate of matrix, and the program request degree of depth is 1cm～1.5cm, and 1, every cave seed covers one deck thin soil in cave panel surface after broadcasting;
Step 5, seedling cultivation greenhouse is cultivated: it is that 25 ℃～35 ℃, the seedling cultivation greenhouse of illumination 15000LUX are cultivated that the cave dish that completes program request is placed in to temperature, in the training period cave dish is carried out to moisturizing, and after 25 days～30 days, seedling grows up to, and can be with soil to transplant.
Further, in step 2, every 8 hours～12 hours, to pallet, add water to keep moistening.
Further, in step 5, every 3 days～5 days, need in 1 meter of eminence, to cave dish, carry out moisturizing with 10000 order shower nozzles in the training period.
The fast seedling-cultivating method of younghusband jerusalemsage root provided by the invention, passes through following steps:
Soak seed, choose the younghusband jerusalemsage root seed of full seed, with 1000 times of 75% tpn dilution, soak 30 minutes, then soak 24 hours with clear water, finally with clear water, rinse once standby;
Insulating box vernalization, is placed in soaked younghusband jerusalemsage root seed in the insulating box that is put into again 25 ℃～35 ℃ in the pallet of completing in advance moistening nonwoven, cultivate 48 hours, during every 8 hours～12 hours, to pallet, add a small amount of water to keep moistening;
The configuration of seedling culture hole plate matrix, uses silt, sheep excrement, pearl salt volume ratio according to 5:3:2 proportional arrangement medium, and paving fills in the dish aperture of cave;
The seed program request of sprouting, will be after 48 hours constant temperature culture, and the seed program request of sprouting is to filling up in the seedling culture hole plate of matrix, and the program request degree of depth is 1cm～1.5cm, 1, every cave seed.After broadcasting, in cave panel surface, cover one deck thin soil;
Seedling cultivation greenhouse is cultivated: it is that 25 ℃～35 ℃, the seedling cultivation greenhouse of illumination 15000LUX are cultivated that the cave dish that completes program request is placed in to temperature, every 3 days～5 days, need in 1 meter of eminence, to cave dish, carry out moisturizing with 10000 order shower nozzles in the training period, after 25 days～30 days, seedling grows up to, and can be with soil to transplant.
The present invention has improved kind shoot survival percent greatly, and seedling raising process is simple, seedling cost is low, specification requirement is low, workable, growing-seedling period is short, equipment requirement is low, can realize large-scale production.
Accompanying drawing explanation
Fig. 1 is the flow chart of the fast seedling-cultivating method of the younghusband jerusalemsage root that provides of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, the fast seedling-cultivating method of the younghusband jerusalemsage root of the embodiment of the present invention comprises the following steps:
S101: soak seed, choose the younghusband jerusalemsage root seed of full seed, soak 30 minutes with 1000 times of 75% tpn dilution, then soak 24 hours with clear water, finally rinse standby with clear water;
S102: insulating box vernalization, soaked younghusband jerusalemsage root seed is placed in the insulating box that is put into again 25 ℃～35 ℃ in the pallet of completing in advance moistening nonwoven, cultivate 48 hours, during every 8 hours～12 hours, to pallet, add a small amount of water to keep moistening;
S103: the configuration of seedling culture hole plate matrix, use silt, sheep excrement, pearl salt volume ratio according to 5:3:2 proportional arrangement medium, and paving fills in the dish aperture of cave;
S104: the seed program request of sprouting, will be after 48 hours constant temperature culture, the seed program request of sprouting is to filling up in the seedling culture hole plate of matrix, and the program request degree of depth is 1cm～1.5cm, 1, every cave seed.After broadcasting, in cave panel surface, cover one deck thin soil;
S105: seedling cultivation greenhouse is cultivated: it is that 25 ℃～35 ℃, the seedling cultivation greenhouse of illumination 15000LUX are cultivated that the cave dish that completes program request is placed in to temperature, every 3 days～5 days, need in 1 meter of eminence, to cave dish, carry out moisturizing with 10000 order shower nozzles in the training period, after 25 days～30 days, seedling grows up to, and can be with soil to transplant.
In step S102, constant temperature vernalization technology: in order to break seed dormancy, we need provide an environmental condition that makes seed body endoenzyme activity excitation, impel its breaking dormancy, starts growth.
In step S104, the program request degree of depth: the program request degree of depth refers to that the seed of program request is apart from the vertical range of cave dish upper port, because different plant characteristics are different, when carrying out the dish sowing of seed cave, need sow according to selected its program request degree of depth of its plant characteristic, so just can reach best nursery effect.As: the vegetables program request degree of depth of leaf vegetables is 0.5cm～1.0cm, and the vegetables program request degree of depth of solanaceous vegetables is 1.5cm～2.0cm;
Before and after the present invention grows up to from vernalization to last seedling, only need one month, growing-seedling period is shorter; In whole seedling raising process, the comparatively meticulous instrument that uses only has an insulating box, thus for equipment require relatively low; Raw materials used is silt, sheep excrement, pearl salt etc., and price is all lower comparatively speaking, so seedling cost of the present invention is also relatively low; In operating process, do not relate to comparatively complicated and meticulous operation, now for ordinary people, operability is stronger, by very strong practicality and replicability; What adopt is current very popular point disk seedling growing mode, and the seedling after incubation can carry out balled transplanting, turns round and look at the survival rate that this can fully ensure transplanting.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. a fast seedling-cultivating method for younghusband jerusalemsage root, is characterized in that, the fast seedling-cultivating method of this younghusband jerusalemsage root comprises the following steps:
Step 1, soaks seed, and chooses the younghusband jerusalemsage root seed of full seed, soaks 30 minutes, then soak 24 hours with clear water with 1000 times of 75% tpn dilution, rinses once standby more afterwards with clear water;
Step 2, insulating box vernalization, is placed in soaked younghusband jerusalemsage root seed in the insulating box that is put into again 25 ℃～35 ℃ in the pallet of completing in advance moistening nonwoven, cultivates 48 hours, to pallet, adds water to keep moistening;
Step 3, the configuration of seedling culture hole plate matrix, by silt, sheep excrement, pearl salt volume ratio, according to 5:3:2 proportional arrangement medium, silt sheep excrement pearl salt is placed in cubing container and according to the volume ratio of volume ratio 5:3:2 and is weighed, after they being stirred with instrument, fully mix, finally paving fills in the dish aperture of cave again;
Step 4, the seed program request of sprouting, will be after 48 hours constant temperature culture, and the seed program request of sprouting is to filling up in the seedling culture hole plate of matrix, and the program request degree of depth is 1cm～1.5cm, and 1, every cave seed covers one deck thin soil in cave panel surface after broadcasting;
Step 5, seedling cultivation greenhouse is cultivated: it is that 25 ℃～35 ℃, the seedling cultivation greenhouse of illumination 15000LUX are cultivated that the cave dish that completes program request is placed in to temperature, in the training period cave dish is carried out to moisturizing, and after 25 days～30 days, seedling grows up to, and can be with soil to transplant.
2. the fast seedling-cultivating method of younghusband jerusalemsage root as claimed in claim 1, is characterized in that, in step 2, every 8 hours～12 hours, to pallet, adds water to keep moistening.
3. the fast seedling-cultivating method of younghusband jerusalemsage root as claimed in claim 1, is characterized in that, in step 5, need in 1 meter of eminence, to cave dish, carry out moisturizing with 10000 order shower nozzles in the training period every 3 days～5 days.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102614269A (en) * | 2012-04-12 | 2012-08-01 | 久美彭措 | Method for preparing traditional Tibetan medicine for treating pharyngitis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102614269A (en) * | 2012-04-12 | 2012-08-01 | 久美彭措 | Method for preparing traditional Tibetan medicine for treating pharyngitis |
Non-Patent Citations (3)
| Title |
|---|
| RYOJI KASAI ET AL: "Iridoid glycosides from Phlomis younghusbandii roots", 《PHYTOCHEMISTRY》, vol. 36, no. 4, 31 December 1994 (1994-12-31), pages 967 - 970, XP026631570, DOI: 10.1016/S0031-9422(00)90473-4 * |
| 曾钰婷等: "利用组织培养方法快速繁殖螃蟹甲技术", 《西藏农业科技》, vol. 31, no. 2, 31 December 2009 (2009-12-31), pages 26 - 28 * |
| 谭丽杰: "糙苏属植物螃蟹甲的研究进展", 《中国现代中药》, vol. 13, no. 7, 30 July 2011 (2011-07-30), pages 52 - 57 * |
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