CN103585462A - Anti-coccidiosis traditional Chinese medicine mixture and preparation method thereof - Google Patents
Anti-coccidiosis traditional Chinese medicine mixture and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of veterinary medicine pharmacy, and particularly relates to an anti-coccidiosis traditional Chinese medicine mixture and a preparation method thereof. The anti-coccidiosis traditional Chinese medicine mixture mainly comprises the traditional Chinese medicine raw materials in parts by weight as follows: 5-20 parts of cooked rhubarb, 5-20 parts of common bletilla pseudobulb, 5-20 parts of garden burnet, 15-40 parts of hairyvein agrimony, 1-10 parts of roots of Chinese pulsatilla and 1-10 parts of antifebrile dichroa. The anti-coccidiosis traditional Chinese medicine mixture and the preparation method thereof have the characteristics of reasonable formula, capacities of increasing medicine absorptivity of poultry and improving the medicine efficacy, small toxic and side effect, low medicine residue, environment friendliness and the like.
Description
technical field
The invention belongs to herbal medicine pharmaceutical technology field, particularly a kind of anticoccidial herb mixture and preparation method thereof.
background technology
The most serious protozoacide of chicken coccidiosis Shi Dui world aviculture harm, a kind of by unicellular parasite Eimeria(Amy ear) cause.Parasite breeds and causes tissue injury at double in small intestinal, reduces the absorbance of nutrient in feed intake and feedstuff, causes dehydration and blood loss.The death loss of coccidiosis in commercialization poultry farming, more than 20%, become a very large threat of poultry cultivation.
Control to chicken coccidiosis, mainly relies on chemosynthesis class or ionophore class coccidiostat at present, but such medicine easily causes worm strain to produce drug resistance, and easily occurs residual; In addition, because live vaccine easily occurs, virulence is anti-by force, DNA vaccination research is complete not to the utmost, therefore both not yet promote the use of.
summary of the invention
For above problem, anticoccidial herb mixture that the object of the present invention is to provide a kind of good effect, have no side effect, has no drug resistance and preparation method thereof.
The present invention is by the following technical solutions:
A herb mixture, is mainly made by the raw material of Chinese medicine of following parts by weight: 5～20 parts of Radix Et Rhizoma Rhei, 5～20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 5～20 parts of Radix Sanguisorbaes, 15～40 parts of Herba Agrimoniaes, 1～10 part of the Radix Pulsatillae, 1～10 part of Radix Dichroae.
Further preferably, described raw material of Chinese medicine and weight portion thereof are: 10～20 parts of Radix Et Rhizoma Rhei, 10～20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 5～15 parts of Radix Sanguisorbaes, 20～40 parts of Herba Agrimoniaes, 1～5 part of the Radix Pulsatillae, 5～10 parts of Radix Dichroaes.
Further preferred, described raw material of Chinese medicine and weight portion thereof are: 15 parts of Radix Et Rhizoma Rhei, 20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 12 parts of Radix Sanguisorbaes, 35 parts of Herba Agrimoniaes, 8 parts of the Radix Pulsatillaes, 10 parts of Radix Dichroaes.
The method of preparing anticoccidial herb mixture, pulverizes raw material of Chinese medicine, decoct and extract rear concentrating under reduced pressure, then, after membrance separation clarification, adds antiseptic, dilution to obtain.
Preparation process specifically comprises the following steps: get in proportion each raw material of Chinese medicine, pulverize as coarse powder, add coarse powder weight 10-12 water doubly, soak 30-60min, decoct 60-120min and extract, extracting solution is cooling, centrifugal, to filter, filtrate is concentrated into the gross weight of former raw material of Chinese medicine, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid successively, in clarification medicinal liquid, add antiseptic and deionized water.
The consumption of antiseptic is 0.05～0.20% of clarification medicinal liquid weight, and the consumption of deionized water is identical with clarification medicinal liquid weight.
The present invention follows Traditional Chinese medical theory, and a kind of anticoccidial herb mixture is provided, and the raw material of anticoccidial herb mixture has been used a series of raw material of Chinese medicine, wherein, and Radix Et Rhizoma Rhei, purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation.Pseudobulbus Bletillae (Rhizoma Bletillae), astringing to arrest bleeding, detumescence and promoting granulation.Radix Sanguisorbae, cooling blood for hemostasis, heat-clearing and toxic substances removing, detumescence sore.Herba Agrimoniae, astringing to arrest bleeding, dysentery relieving, parasite killing.The Radix Pulsatillae, heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, removing dampness and killing parasites.Radix Dichroae, expectorant, preventing the attack (or recurrence) of malaria, treating malaria disease, scrofula.Each Chinese medicinal components reasonable compatibility, complements each other above, plays altogether the effect of anthelmintic, promoting blood circulation and hemostasis, invigorating qi and benefiting blood, heat-clearing and toxic substances removing, by oral administration, easy to use, is beneficial to absorption, and effect is remarkable.
This anticoccidial herb mixture is the liquid of rufous clear, first that medical material is broken in preparation process, is conducive to effective ingredient stripping; Adopt traditional handicraft to decoct and form, guaranteed, between each herbal medicine, various useful biochemistry and chemical reaction at high temperature occur, guaranteed the effectiveness of medicine; Continuous circumfluence extraction, energy savings, has guaranteed the preservation of effective ingredient; Adopt the remove impurity of membrane filtration isolation technics, than conventional alcohol deposition method, retained effective ingredient, guarantee the stability of product, and can enhance productivity, shorten the production cycle, reduce production costs.
Generally, reasonable recipe of the present invention, by technological transformation, removes impurity, increased fowl drug absorption rate, improved the curative effect of medicine; By drinking-water administration, be conducive to intensive, large-scale cultivation; Raw material Chinese medicine majority is from natural, and chemical composition is various, and cause of disease is difficult for producing drug resistance, the clinical verification that curative effect process is permanent, and there is the features such as toxic and side effects is little, drug residue is low, environmentally friendly.
the specific embodiment
With specific embodiment, technical scheme of the present invention is described below, but protection scope of the present invention is not limited to this:
Embodiment 1
Take medical material: Radix Et Rhizoma Rhei 15g, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 g, Radix Sanguisorbae 12 g, Herba Agrimoniae 35 g, the Radix Pulsatillae 8 g, Radix Dichroae 10 g, above-mentioned crude drug is ground into coarse powder, add 1000g water, soak 60 minutes, decoct and extract 60 minutes; It is centrifugal 10 minutes of 15000rpm by high speed centrifuge rotating speed that extracting solution is cooled to room temperature, after filtering, filtrate is concentrated into 100g, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid again, the potassium sorbate that adds clarification medicinal liquid weight 0.05%, add and deionized water that clarification medicinal liquid etc. is heavy, canned and get final product.
Embodiment 2
Take medical material: Radix Et Rhizoma Rhei 5 g, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 g, Radix Sanguisorbae 20 g, Herba Agrimoniae 40 g, the Radix Pulsatillae 10 g, Radix Dichroae 5 g, above-mentioned crude drug is ground into coarse powder, add 1200g water, soak 45 minutes, decoct and extract 120 minutes; It is centrifugal 15 minutes of 10000rpm by high speed centrifuge rotating speed that extracting solution is cooled to room temperature, after filtering, filtrate is concentrated into 100g, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid again, the potassium sorbate that adds clarification medicinal liquid weight 0.05%, add and deionized water that clarification medicinal liquid etc. is heavy, canned and get final product.
Embodiment 3
Take medical material: Radix Et Rhizoma Rhei 20g, Pseudobulbus Bletillae (Rhizoma Bletillae) 15g, Radix Sanguisorbae 20g, Herba Agrimoniae 35g, Radix Pulsatillae 1g, Radix Dichroae 9g, above-mentioned crude drug is ground into coarse powder, add 1000g water, soak 60 minutes, decoct and extract 1h; It is centrifugal 30 minutes of 8000rpm by high speed centrifuge rotating speed that extracting solution is cooled to room temperature, after filtering, filtrate is concentrated into 100g, medicinal liquid filters and obtains clarifying medicinal liquid through ceramic micro filter film, hollow fiber ultrafiltration membrane again, the potassium sorbate that adds clarification medicinal liquid weight 0.1%, add and deionized water that clarification medicinal liquid etc. is heavy, canned and get final product.
Embodiment 4
Take medical material: Radix Et Rhizoma Rhei 5g, Pseudobulbus Bletillae (Rhizoma Bletillae) 15g, Radix Sanguisorbae 5g, Herba Agrimoniae 40g, Radix Pulsatillae 10g, Radix Dichroae 10g, above-mentioned crude drug is ground into coarse powder, add 1000g water, soak 30 minutes, decoct and extract 30 minutes; It is centrifugal 30 minutes of 8000rpm by high speed centrifuge rotating speed that extracting solution is cooled to room temperature, after filtering, filtrate is concentrated into 85g, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid again, the potassium sorbate that adds clarification medicinal liquid weight 0.05%, add and deionized water that clarification medicinal liquid etc. is heavy, canned and get final product.
Embodiment 5
Take medical material: Radix Et Rhizoma Rhei 15g, Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Radix Sanguisorbae 20g, Herba Agrimoniae 20g, Radix Pulsatillae 10g, Radix Dichroae 10g, above-mentioned crude drug is ground into coarse powder, add 960g water, soak 30 minutes, decoct and extract 30 minutes; It is centrifugal 10 minutes of 15000rpm by high speed centrifuge rotating speed that extracting solution is cooled to room temperature, after filtering, filtrate is concentrated into 80g, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid again, the potassium sorbate that adds clarification medicinal liquid weight 0.1%, add and deionized water that clarification medicinal liquid etc. is heavy, canned and get final product.
Effect experiment:
1. test grouping and processing
When test chicken 9 age in days, select 250 of the close persons of body weight, be divided at random 5 groups, 10 every group, establish 5 repetitions.Wherein A group is for healthy group; B～E group when 16 age in days, peroral infection Sporulated Eimeria Tenella (8 * 10
4individual/plumage) carry out coccidiosis case and copy, wherein B group is model group, not medication after infection; C group is the embodiment of the present invention 1 anticoccidial herb mixture, and 96h after infection, adds 4 g/L in drinking-water, be used in conjunction 4d; During D, E group are respectively, western medicine group, 96h after infection, adds respectively chicken coccidia powder (purchased from Taian Shandong Hai Na pharmaceutcal corporation, Ltd) 6 g/L and Embazin (Baoding Jizhong Pharmaceutical Co., Ltd.) 0.5g/L in drinking-water, be used in conjunction 4d.After four days, start to observe.
2. detect index
(1) clinical symptoms: duration of test is observed the performance of feces state, hemafecia degree and chickling every day, the promoting the circulation of blood of going forward side by side of noting down is just scored.
(2) egg capsule is discharged situation: duration of test 4th～8d, and the feces of draining in each group 1d is fully mixed, and take 2g at random, adopt Cecil McMaster method to carry out a gram feces egg capsule counting.
(3) survival rate: duration of test, to each group because of the dead chicken counting of chicken coccidiosis.Each group survival chicken number and each are organized total chicken and are counted ratio and be survival rate.
Survival rate (%)=
* 100%
(4) pathological changes value: test the 9th day, each organizes chicken by the execution of only weighing, and cuts open and gets caecum, carries out caecum lesion and scores.
Table 1 pathological changes value standards of grading
| Mark | Performance |
| 4 minutes | In caecum, be full of a large amount of blood and intestinal core and caecum enlargement contains excrement slag or do not contain in intestinal core; Dead |
| 3 minutes | In caecum, have volume blood and have caecum core (the caseous Banana Type block of blood clot or canescence), caecum wall is obviously plump, and in caecum, feces content is few |
| 2 minutes | Pathological changes quantity is more, and cecal content is obviously with blood, and caecum wall slightly thickens, and content is normal |
| 1 minute | Caecum wall has the petechiae that seldom amount is dispersed in, and intestinal wall does not thicken, and content is normal |
| 0 minute | Without naked eyes pathological changes |
(5) egg capsule value: test the 9th day, each organizes chicken by the execution of only weighing, takes out cecal content, adopts McMaster ' s method to calculate gram feces egg sac number of cecal content, and than number, judges egg capsule value according to egg capsule.
Egg capsule than number (%)=
* 100%
Table 2 egg capsule value standards of grading
| Mark | Performance |
| 0 | Egg capsule is 0%～1% than number |
| 5 | Egg capsule is 1%～25% than number |
| 10 | Egg capsule is 26%～50% than number |
| 20 | Egg capsule is 51%～75% than number |
| 40 | Egg capsule is 76%～100% than number |
(6) the relative weight gain rate: before test 1d, test the 9th day, each organizes chicken by only on an empty stomach weighing,
The relative weight gain rate (%)=infected group average weight gain/Health survey group average weight gain.
(7) criterion calculation of anticoccidial index ，Yi Merck company, that is:
Anticoccidial index=(the relative weight gain rate+survival rate)-(pathological changes value+egg capsule value).
Efficacy determination: be greater than at 180 o'clock, be judged to be curative effect outstanding; Be 160～180 o'clock, be judged to be curative effect good; Be 120～160 o'clock, be judged to be curative effect medium; Be less than at 120 o'clock, be judged to be weak curative effect.
experimental result:
Anticoccidial index
Table 3 is respectively organized anticoccidial index
| Group | The relative weight gain rate (%) | Survival rate (%) | Egg capsule value | Pathological changes value | Anticoccidial index |
| A group | 100 | 100 | 0 | 0 | 200 |
| B group | 57.43 | 93 | 40 | 25.0 | 84.92 |
| C group | 92.13 | 100 | 0 | 13.5 | 176.20 |
| D group | 74.73 | 91 | 10 | 21.7 | 134.56 |
| E group | 80.50 | 100 | 0 | 21.0 | 161.28 |
Experimental studies results shows, anticoccidial herb mixture drinking-water of the present invention medicine for treatment has significant therapeutic effect to chicken coccidiosis, and anticoccidial index has reached 176.2; Can significantly alleviate the infringement of coccidiosis to kidney, liver, caecum and fabricius bursa.
Claims (6)
1. an anticoccidial herb mixture, is characterized in that, mainly by the raw material of Chinese medicine of following parts by weight, is made: 5～20 parts of Radix Et Rhizoma Rhei, 5～20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 5～20 parts of Radix Sanguisorbaes, 15～40 parts of Herba Agrimoniaes, 1～10 part of the Radix Pulsatillae, 1～10 part of Radix Dichroae.
2. anticoccidial herb mixture as claimed in claim 1, is characterized in that, described raw material of Chinese medicine and weight portion thereof are: 10～20 parts of Radix Et Rhizoma Rhei, 10～20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 5～15 parts of Radix Sanguisorbaes, 20～40 parts of Herba Agrimoniaes, 1～5 part of the Radix Pulsatillae, 5～10 parts of Radix Dichroaes.
3. anticoccidial herb mixture as claimed in claim 1 or 2, is characterized in that, described raw material of Chinese medicine and weight portion thereof are: 15 parts of Radix Et Rhizoma Rhei, 20 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 12 parts of Radix Sanguisorbaes, 35 parts of Herba Agrimoniaes, 8 parts of the Radix Pulsatillaes, 10 parts of Radix Dichroaes.
4. the method for the anticoccidial herb mixture described in preparation claim 1-3 any one, is characterized in that, raw material of Chinese medicine is pulverized, decocted and extract rear concentrating under reduced pressure, then, after membrance separation clarification, adds antiseptic, dilution to obtain.
5. the method for preparing anticoccidial herb mixture as claimed in claim 4, is characterized in that, specifically comprises the following steps: get in proportion each raw material of Chinese medicine, pulverize as coarse powder, add coarse powder weight 10-12 water doubly, soak 30-60min, decoct 60-120min and extract, extracting solution is cooling, centrifugal, to filter, filtrate is concentrated into the gross weight of former raw material of Chinese medicine, through ceramic micro filter film, hollow fiber ultrafiltration membrane, filter and obtain clarifying medicinal liquid successively, in clarification medicinal liquid, add antiseptic and deionized water.
6. the method for preparing anticoccidial herb mixture as claimed in claim 5, is characterized in that, the consumption of antiseptic is 0.05～0.20% of clarification medicinal liquid weight, and the consumption of deionized water is identical with clarification medicinal liquid weight.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100788A (en) * | 2009-12-16 | 2011-06-22 | 青岛康地恩药业有限公司 | Chinese medicinal preparation for preventing and treating chicken coccidiosis |
| CN102319309A (en) * | 2011-08-11 | 2012-01-18 | 福建农林大学 | Traditional Chinese medicine preparation for treating chicken coccidiosis |
| CN102885914A (en) * | 2012-10-30 | 2013-01-23 | 天津生机集团股份有限公司 | Traditional Chinese medicine oral liquid for treating chicken coccidiosis and preparation method thereof |
| CN102988764A (en) * | 2012-12-19 | 2013-03-27 | 西安市昌盛动物保健品有限公司 | Chinese medicinal preparation for resisting coccidiosis and preparation method thereof |
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2013
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100788A (en) * | 2009-12-16 | 2011-06-22 | 青岛康地恩药业有限公司 | Chinese medicinal preparation for preventing and treating chicken coccidiosis |
| CN102319309A (en) * | 2011-08-11 | 2012-01-18 | 福建农林大学 | Traditional Chinese medicine preparation for treating chicken coccidiosis |
| CN102885914A (en) * | 2012-10-30 | 2013-01-23 | 天津生机集团股份有限公司 | Traditional Chinese medicine oral liquid for treating chicken coccidiosis and preparation method thereof |
| CN102988764A (en) * | 2012-12-19 | 2013-03-27 | 西安市昌盛动物保健品有限公司 | Chinese medicinal preparation for resisting coccidiosis and preparation method thereof |
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| CN103585462B (en) | 2016-01-13 |
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Denomination of invention: The invention relates to an anti coccidia traditional Chinese medicine mixture and a preparation method thereof Effective date of registration: 20211224 Granted publication date: 20160113 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd. Registration number: Y2021980016170 |
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Denomination of invention: A Chinese herbal medicine mixture for anti coccidiosis and its preparation method Granted publication date: 20160113 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980004821 |
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