Background technology
Cholecystitis is the gallbladder inflammatory lesion that bacterial infection or chemical irritation (bile component change) cause, is the commonly encountered diseases of gallbladder.In abdominal surgery, its sickness rate is only second to appendicitis, and primary disease is more common in the middle age of 35~55 years old, and women's morbidity is many compared with male, is especially more common in the women of obesity and multiple pregnancies.According to the clinical performance of anxious mitigation of morbidity, can be divided into acute and chronic two classes, all belong to gallbladder system and infect category.The symptom of acute cholecystitis, mainly contains upper right abdomen pain, feels sick, vomiting and heating etc.Acute cholecystitis can cause right Upper abdominal pain, pain and biliary colic are closely similar at the beginning, but its lasting time of the stomachache that acute cholecystitis causes is often longer, breathe and change position usually to make pain increase the weight of, therefore patient likes reposing to the right more, to alleviate abdomen pain.Some patient has nausea and vomiting, but vomiting is generally inviolent.Most patient is also with heating, and body temperature is conventionally between 38.0 ℃~38.5 ℃, and Gao Re and shiver with cold are actually rare.A few patients also has the white of the eye and skin slightly to turn to be yellow.When doctor checks patient's abdominal part, can find that right upper quadrant of the abdomen has tenderness, and have tension of abdominal muscle, in 1/3 patient, can also touch the gallbladder of enlargement greatly.Chemical examination patient's blood, can find numeration of leukocyte and neutrophilia in most human bloods.Ultrasound diagnosis can find that gallbladder enlargement, cyst wall thicken, and visible calculus gets lodged in the cervical region of gallbladder.According to above symptom, physical examination and various auxiliary examination, doctor generally can make the diagnosis of acute cholecystitis in time.
Western medical treatment divides expectant treatment and operative treatment.Expectant treatment, to lie up, is strengthened nutrition, and medicine adopts antibiotics intravenous drip more, and indometacin is oral, though obtained the good effect of short-term, easily repeatedly; Operative treatment is excision cholecystitis, but the mortality rate 0.5-3% of operative therapy, jaundice case mortality is 10-15%, and gerontal case mortality rate is up to 13.5-16%, and operation intercurrent disease is about 15%.Therefore operative therapy is unsafty as the basic therapy for the treatment of cholecystitis.The Chinese medicine for the treatment of cholecystitis mainly contains at present, Decoction of Gentiana for Purging the Liver-fire, depressed liver-energy dispersing and function of gallbladder promoting soup, soothing liver-QI Stomach-Regulating Decoction, stomach invigorating circulation of qi promoting soup, XIAOYAN LIDAN PIAN, depressed liver-energy dispersing and function of gallbladder promoting cream, clearing gallbladder circulation of qi promoting soup, clearing away heat-damp and promoting diuresis soup, clearing gallbladder pathogenic fire purging soup, bupleurum root decoction, Herba Artemisiae Scopariae soup adjust that five a kind of reed mentioned in ancient books are loose, Herba Artemisiae Scopariae and CHENGQI TANG, Daxianxiong Tang etc., although these medicines have certain curative effect, but still can not be satisfactory.
Chrysosplenium nudicaule Bunge is saxifragaceae plant Chrysasplenium nudicaule Chrysosplenium nudicaule Bge. and the dry herb that belongs to several plants together.Gather autumn, removes dead leaf, cleans, and dries.Record in < < Drug Standard of Ministry of Public Health of the Peoples Republic of China Tibetan medicine (first) > >, standard number: WS3-BC-0061-95.Cold in nature, bitter in the mouth.There is clearing heat secreting bile, cathartic purgation effect.For gallbladder-heat disease, fever, headache, cholecystitis, cholelithiasis.For rushing down gallbladder-heat disease common drug.For China, hide the common drug of the minority areas such as illiteracy.But the basic research of relevant Chrysosplenium nudicaule Bunge is still very limited, the follow-up promotion and application of this medical material are restricted.Modern study rarely has report about chemical composition and the pharmacological action of Herba of Artemisiae admsii.
Domestic patent search result, has no Chrysosplenium nudicaule Bunge Patents.
Above-mentioned document and patent etc., there is not yet Chrysosplenium nudicaule Bunge method for preparing extractive and preparing the relevant report for the treatment of the application in acute cholecystitis medicine.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and and the application in preparation treatment acute cholecystitis medicine of Chrysosplenium nudicaule Bunge extract.
The present invention is achieved through the following technical solutions:
The present invention is raw materials used: Chrysosplenium nudicaule Bunge is little saxifragaceae plant Chrysasplenium nudicaule Chrysosplenium nudicaule Bge. and the dry herb that belongs to several plants together.
the present inventionthe preparation method of extract and the application in preparation treatment acute cholecystitis medicine thereof, described Chrysosplenium nudicaule Bunge method for preparing extractive is:
(1) Chrysosplenium nudicaule Bunge, soaks 4-12 hour, adopts extraction by steam distillation volatile oil, extraction time 1-4 hour, and extraction time 1-3 time, amount of water is 8-15 times of Chrysosplenium nudicaule Bunge weight, collects volatile oil, obtains Chrysosplenium nudicaule Bunge extract A; Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(2) step (1) being obtained to medicinal residues A is solvent with concentration 50%-95% ethanol, heating and refluxing extraction, extraction time is 1-3 time, each extraction time is 1-3 hour, and the 8-15 that each solvent load is Chrysosplenium nudicaule Bunge weight doubly, filters, extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(3) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.10-1.20, filters, and medicinal liquid, by nonpolar macroporous adsorption resin, first washes with water, and water elution liquid is directly by polar macroporous resin; Use the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 30%-70% again, collect different concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting polar macroporous adsorption resin of concentration 50%-95% again, collect different concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4), by above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D, wherein one or both or three kinds or four kinds mix by a certain percentage, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
A kind of Chrysosplenium nudicaule Bunge method for preparing extractive is:
(1) Chrysosplenium nudicaule Bunge, soaks 4-12 hour, adopts extraction by steam distillation volatile oil, extraction time 1-4 hour, and extraction time 1-3 time, amount of water is 8-15 times of Chrysosplenium nudicaule Bunge weight, collects volatile oil, obtains Chrysosplenium nudicaule Bunge extract A; Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(2) step (1) being obtained to medicinal residues A is solvent with concentration 50%-95% ethanol, heating and refluxing extraction, extraction time is 1-3 time, each extraction time is 1-3 hour, and the 8-15 that each solvent load is Chrysosplenium nudicaule Bunge weight doubly, filters, extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(3) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.10-1.20, filters, and medicinal liquid, by nonpolar macroporous adsorption resin, first washes with water, and water elution liquid is directly by polar macroporous resin; Use the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 30%-70% again, collect different concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting polar macroporous adsorption resin of concentration 50%-95% again, collect different concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4) above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D are mixed, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
Preferred a kind of Chrysosplenium nudicaule Bunge method for preparing extractive is:
(1) Chrysosplenium nudicaule Bunge, soaks 6 hours, adopts extraction by steam distillation volatile oil, 2 hours extraction times, and extraction time 2 times, amount of water is 12 times of Chrysosplenium nudicaule Bunge weight, collects volatile oil, obtains Chrysosplenium nudicaule Bunge extract A; Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(2) step (1) being obtained to medicinal residues A is solvent with concentration 60% ethanol, heating and refluxing extraction, and extraction time is 2 times, each extraction time is 2 hours, each solvent load is 12 times of Chrysosplenium nudicaule Bunge weight, filters, and extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(3) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.12, filters, and medicinal liquid, by D101 nonpolar macroporous adsorption resin, first washes the polar macroporous resin of DM130 that water elution liquid directly passes through with water; Use the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 60% again, collect 60% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting DM130 polar macroporous adsorption resin of concentration 75% again, collect 75% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4) above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D are mixed, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
The preparation method of a kind of Chrysosplenium nudicaule Bunge extract of the present invention, is characterized in that: the nonpolar macroporous adsorption resin adopting is D101 macroporous adsorbent resin, AB-8; The polar macroporous adsorption resin adopting is DM130 macroporous adsorbent resin, ADS-17 macroporous adsorbent resin.
The preparation method of a kind of Chrysosplenium nudicaule Bunge extract of the present invention and application, the described application being applied as in preparation treatment acute cholecystitis medicine.
The treatment acute cholecystitis medicine that Chrysosplenium nudicaule Bunge extract of the present invention and chemical drugs or Chinese medicine or natural drug form.
Chrysosplenium nudicaule Bunge extract A of the present invention, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D, the application in preparation treatment acute cholecystitis medicine.
The treatment acute cholecystitis medicine that Chrysosplenium nudicaule Bunge extract A of the present invention, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D and chemical drugs or Chinese medicine or natural drug form.
Chrysosplenium nudicaule Bunge extract of the present invention, by the various adjuvants that add pharmaceutics to allow, makes the peroral dosage forms such as tablet on pharmaceutics, granule, capsule.
The specific embodiment
Below by specific experiment example and embodiment, the preparation method of a kind of Chrysosplenium nudicaule Bunge extract of the present invention and the application in preparation treatment acute cholecystitis medicine thereof are described further, but are not limited to the present invention.
Below by specific embodiment, be described in further detail.
Embodiment 1: the preparation of Chrysosplenium nudicaule Bunge extract
(1) Chrysosplenium nudicaule Bunge 18kg, soak 6 hours, adopt extraction by steam distillation volatile oil, 2 hours extraction times, extraction time 2 times, amount of water is 12 times of Chrysosplenium nudicaule Bunge weight, collect volatile oil, obtain Chrysosplenium nudicaule Bunge extract A, and Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(3) step (1) being obtained to medicinal residues A is solvent with concentration 60% ethanol, heating and refluxing extraction, and extraction time is 2 times, each extraction time is 2 hours, each solvent load is 12 times of Chrysosplenium nudicaule Bunge weight, filters, and extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(2) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.12, filters, and medicinal liquid, by D101 nonpolar macroporous adsorption resin, first washes the polar macroporous resin of DM130 that water elution liquid directly passes through with water; Use the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 60% again, collect 60% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting DM130 polar macroporous adsorption resin of concentration 75% again, collect 75% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4) above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D are mixed, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
Embodiment 2: the preparation of Chrysosplenium nudicaule Bunge extract
(1) Chrysosplenium nudicaule Bunge 22kg, soak 4 hours, adopt extraction by steam distillation volatile oil, 1 hour extraction time, extraction time 3 times, amount of water is 8 times of Chrysosplenium nudicaule Bunge weight, collect volatile oil, obtain Chrysosplenium nudicaule Bunge extract A, and Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(3) step (1) being obtained to medicinal residues A is solvent with concentration 50% ethanol, heating and refluxing extraction, and extraction time is 1 time, each extraction time is 3 hours, each solvent load is 8 times of Chrysosplenium nudicaule Bunge weight, filters, and extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(2) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.20, filters, and medicinal liquid, by AB-8 nonpolar macroporous adsorption resin, first washes with water, and water elution liquid is directly by the polar macroporous resin of ADS-17; Use the alcoholic solution eluting AB-8 nonpolar macroporous adsorption resin of concentration 30% again, collect 30% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting ADS-17 polar macroporous adsorption resin of concentration 50% again, collect 50% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4) above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D are mixed, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
Embodiment 3: the preparation of Chrysosplenium nudicaule Bunge extract
(1) Chrysosplenium nudicaule Bunge 21kg, soak 12 hours, adopt extraction by steam distillation volatile oil, 4 hours extraction times, extraction time 1 time, amount of water is 15 times of Chrysosplenium nudicaule Bunge weight, collect volatile oil, obtain Chrysosplenium nudicaule Bunge extract A, and Chrysosplenium nudicaule Bunge aqueous extract is filtered, obtain respectively medicinal liquid A and medicinal residues A;
(3) step (1) being obtained to medicinal residues A is solvent with concentration 95% ethanol, heating and refluxing extraction, and extraction time is 3 times, each extraction time is 1 hour, each solvent load is 15 times of Chrysosplenium nudicaule Bunge weight, filters, and extracting solution reclaims ethanol, concentrated, dry, obtain Chrysosplenium nudicaule Bunge extract B;
(2) medicinal liquid A step (1) being obtained, is concentrated into relative density d=1.10, filters, and medicinal liquid, by D101 nonpolar macroporous adsorption resin, first washes with water, and water elution liquid is directly by the polar macroporous resin of DM130; Use the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 70% again, collect 70% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract C; Use the alcoholic solution eluting DM130 polar macroporous adsorption resin of concentration 95% again, collect 95% concentration ethanol eluent, concentrate drying, obtains Chrysosplenium nudicaule Bunge extract D;
(4) above-mentioned Chrysosplenium nudicaule Bunge extract A, Chrysosplenium nudicaule Bunge extract B, Chrysosplenium nudicaule Bunge extract C, Chrysosplenium nudicaule Bunge extract D are mixed, obtain Chrysosplenium nudicaule Bunge extract of the present invention.
Embodiment 4: the preparation of Chrysosplenium nudicaule Bunge extract tablet
Get embodiment 1 Chrysosplenium nudicaule Bunge extract 265g, add starch 60g, mix, granulate, dry, sieve, add microcrystalline Cellulose 15g, magnesium stearate 0.5g, mixes, and is pressed into 1000, obtains Chrysosplenium nudicaule Bunge extract tablet.
Embodiment 5: the preparation of Chrysosplenium nudicaule Bunge extract particles agent
Get embodiment 2 Chrysosplenium nudicaule Bunge extract 265g, add dextrin 100g, mix, granulate, dry, granulate, obtains the agent of Chrysosplenium nudicaule Bunge extract particles.
Embodiment 6: the preparation of Chrysosplenium nudicaule Bunge extract capsule
Get embodiment 3 Chrysosplenium nudicaule Bunge extract 245g, add starch 85g, mix, granulate, dry, granulate,, obtains Chrysosplenium nudicaule Bunge extract capsule by encapsulated 1000.
Experimental example 1: treatment acute cholecystitis
Case enters group standard
Though traditional medicine is without the name of disease of cholecystitis, but its sick disease and diagnosis are early recognized and are recorded, the clinical characters of primary disease is upper right abdomen and the side of body costalgia or epigastrium pit of the stomach place pain, heating or alternate attack of chill and fever or with jaundice often have epigastric distension and distress, feel sick, the symptom such as heating installation, distention and fullness in the abdomen.Therefore in the disease such as the hypochondriac pain of traditional medicine, gastropathy, irritability pain, incoordination between the liver and stomach, jaundice, blocked-up chest Shaoyang disease, gallbladder-distention, distension related to the liver, acute and chronic cholecystitis, cholangitis have been comprised.
Diagnostic criteria: the sudden pain of upper right abdomen, and take on back radiation to the right, and with heating, nauseating, vomiting, health check-up upper right abdominal pressure pain and flesh are defended, and murphy sign is positive, and numeration of leukocyte increases, and B ultrasonic shows gallbladder wall edema, can be diagnosed as acute cholecystitis.
Close case 40 examples of the standard of stating of selector, male's 20 examples wherein, women's 20 examples, 36 years old mean age.
therapeutic scheme:treatment group gives embodiment 4 Chrysosplenium nudicaule Bunge extract tablets, and it is a course for the treatment of that light disease is taken medicine 5-7 days, 2 courses for the treatment of of patient with severe symptoms.
the standard of curative effect evaluation
recovery from illness:the symptoms such as right Upper abdominal pain, nausea and vomiting disappear completely; Have a medical check-up upper right abdomen without tenderness, do not touch gallbladder-murphy sign negative; Ultrasound diagnosis gallbladder is normal, shrinks good.
take a turn for the better:right Upper abdominal pain obviously alleviates, occasionally has nauseating, but without vomiting, bitter taste, the light tenderness of upper right abdomen of having a medical check-up, can touch great gallbladder sometimes, and Boas' sign is negative; Numeration of leukocyte and neutrophilic granulocyte are normal; Ultrasound diagnosis gallbladder is bigger.
invalid:general Symptoms is without improvement.
therapeutic outcome:transference cure in light disease 7 days, transference cure in general 14 days of patient with severe symptoms, blood routine examination is normal.B ultrasonic shows that gallbladder wall edema disappears.Effective percentage 100% by statistics, cure rate 87.5%.
Model case:
1, king * *, female, 35 years old, autumn in 2011 was ill, when medical, the sudden pain of upper right abdomen, and take on back radiation to the right, sharp ache, defecates rare.Once quiet metronidazole, oral indometacin 3 days, within 1 day, through taking 7 of medicines of the present invention, pain relief also progressively disappears, recurrence so far again repeatedly in drug withdrawal.
2, open * *, man, 45 years old, main suit when medical: the sudden pain of upper right abdomen of repeatedly showing effect, companion feels sick, vomiting.Have a medical check-up: upper right abdominal pressure pain and flesh are defended, and murphy sign is positive, ultrasound diagnosis gallbladder wall edema, through taking 10 of medicines of the present invention, pain disappears, ultrasound diagnosis: gallbladder wall edema disappears, so far not recurrence.