CN103919824B - The plant extract of a kind of blood fat reducing and the application in preparing medicine or health product thereof - Google Patents
The plant extract of a kind of blood fat reducing and the application in preparing medicine or health product thereof Download PDFInfo
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Abstract
The invention belongs to plant extract field, relate to a kind of Chrysosplenium nudicaule seed extract with effect for reducing blood fat.This extract is adopted and is prepared with the following method: Chrysasplenium nudicaule is pulverized by (1), after putting drying in oven, adds 70%～90% acetone, and in water-bath reflux, extract, extracting solution reclaims acetone, obtains Chrysasplenium nudicaule total extract extractum；(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under water bath condition, filter, after filtrate concentrating under reduced pressure in a water bath, carry out chromatography；(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7～9, loading volume 6BV～8BV, washing 4BV～6BV remove impurity, with ethyl acetate: the eluant 7BV～9BV eluting of acetone=100:1, flow velocity is 2BV/h～4BV/h, collect eluent, eluent concentrates, dry, to obtain final product.
Description
Technical field
The invention belongs to plant extract field, relate to a kind of Chrysosplenium nudicaule seed extract with effect for reducing blood fat.
Background technology
Blood fat is the general name of contained lipid in human plasma, including cholesterol, triglyceride, cholesterol ester, beta lipoprotein, phospholipid, not esterified fat acid etc..When serum cholesterol exceedes normal value 230 milligrams/100 milliliters, triglyceride more than 140 milligrams/100 milliliters, beta lipoprotein more than 390 milligrams/more than 100 milliliters time, hyperlipidemia can be referred to as.
The cause of disease of hyperlipidemia:
(1) Primary hyperlipemia, it is simply that originally without any other disease, hyperlipemia occurs, is usually because of inherited genetic factors.
(2) Secondary Hyperlipidemia, it is simply that due to the hyperlipemia that a variety of causes causes, such as diabetes, hypothyroidism, nephrotic syndrome, renal transplantation, biliary obstruction etc..
(3) Bad Eating Habit hyperlipemia together, as eating and drinking too much at one meal, be addicted to drink, monophagia, diet are irregular.
(4) certain drug induced hyperlipemia of long-term taking, such as contraceptive, hormone medicine etc..
(5) hyperlipemia that Nervous and Mental Factors causes, it is simply that due to long-term psychentonia, cause endocrine and metabolic disorders, forms hyperlipemia year in and year out.
The mode of prevention hyperlipemia:
(1) reasonable diet: reduce the absorption level of metabolism of lipid and cholesterol, controls body weight, it is prevented that or correct obesity, diuresis row's sodium, regulate blood volume, protect the heart, brain, Renal vascular systemic-function.Adopt low fat low cholesterol, low sodium, homovitamin, appropriate protein and energy diets.
(2) select suitable sports events: according to own situation, select rational sports events.
(3) exercise intensity is grasped: exercise heart rate is the 60～70% of my maximum heart rate, is approximately equivalent to the maximal oxygen uptake of 50～60%.Within general 40 years old, Rate control is at 140 beats/min；50 years old 130 beats/min；Within more than 60 years old, it is advisable within 120 beats/min.
(4) suitable motion frequency: middle-aged and elderly people, particularly old people reduce due to organism metabolism level, the time lengthening recovered after fatigue, therefore motion frequency can optionally increase and decrease, and is generally advisable for 3～4 times weekly.
(5) suitable movement time: movement time controls at 30～40 minutes every time, afternoon, motion was best, and should adhere to motion exercise all the year round.
Chrysasplenium nudicaule, source: Saxifragaceae Chrysasplenium nudicaule ChrysospleniumnudicauleBunge, with all herbal medicine.Perennial herb is high 4.5～10 centimetres.Stem dredges raw brown pubescence or nipple projection, generally without leaf.Basal leaf tool long handle, blade keratin, kidney shape, it is about 9 millimeters, wide about 13 millimeters, edge has shallow tooth, and (tooth is oblate, is about 3 millimeters, wide about 4 millimeters, first concave end and tool 1 wart point, generally mutually folded knot), two sides is without hair, between cog sinus place tool brown pubescence or nipple projection；Petiole length 1～7.5 centimetre, raw brown pubescence is dredged in bottom.Distributed areas: Qinghai, Gansu, Yunnan, Xinjiang, Tibet.Nature and flavor: micro-hardship, cold.Function cures mainly: function of gallbladder promoting, preventing or arresting vomiting.Treatment jaundice and multiple gallbladder disease, tell yellow fluid.
The invention provides a kind of Chrysosplenium nudicaule seed extract treating hyperlipidemia, research proves that this extract has obvious effect for reducing blood fat.
Summary of the invention
It is an object of the invention to provide a kind of Chrysosplenium nudicaule seed extract.
The preparation method that it is a further object of the present invention to provide this extract.
The present invention also provides for the application in preparation treatment hyperlipidemia and vascular hypertension medicine of this extract.
It is an object of the invention to be accomplished by:
A kind of Chrysosplenium nudicaule seed extract with effect for reducing blood fat, this Chrysosplenium nudicaule seed extract is adopted and is prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 40 DEG C～50 DEG C dry 3h～5h after, add 15～25 times amount 70%～90% acetone, in 60 DEG C～70 DEG C water-bath reflux, extract, 2 times～4 times, each 70min～80min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 40 DEG C～50 DEG C water bath condition, filtering, filtrate carries out chromatography after concentrating under reduced pressure in 40 DEG C～50 DEG C water-baths；
(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7～9, loading volume 6BV～8BV, washing 4BV～6BV remove impurity, with ethyl acetate: the eluant 7BV～9BV eluting of acetone=100:1, flow velocity is 2BV/h～4BV/h, collect eluent, eluent concentrates, dry, to obtain final product.
Described Chrysosplenium nudicaule seed extract is preferably adopted and to be prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 40 DEG C dry 5h after, add 15 times amount 90% acetone, in 60 DEG C of water-bath reflux, extract, 4 times, each 70min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 50 DEG C of water bath condition, filtering, filtrate carries out chromatography in 50 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7, loading volume 8BV, washes 4BV remove impurity, with ethyl acetate: the eluant 9BV eluting of acetone=100:1, flow velocity is 2BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
Described Chrysosplenium nudicaule seed extract can also preferably employ following method to be prepared:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 50 DEG C dry 3h after, add 25 times amount 70% acetone, in 70 DEG C of water-bath reflux, extract, 2 times, each 80min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 40 DEG C of water bath condition, filtering, filtrate carries out chromatography in 40 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:9, loading volume 6BV, washes 6BV remove impurity, with ethyl acetate: the eluant 7BV eluting of acetone=100:1, flow velocity is 4BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
Described Chrysosplenium nudicaule seed extract can also preferably employ following method to be prepared:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 45 DEG C dry 4h after, add 20 times amount 80% acetone, in 65 DEG C of water-bath reflux, extract, 3 times, each 75min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 45 DEG C of water bath condition, filtering, filtrate carries out chromatography in 45 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:8, loading volume 7BV, washes 5BV remove impurity, with ethyl acetate: the eluant 8BV eluting of acetone=100:1, flow velocity is 3BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
Described Chrysosplenium nudicaule seed extract, it is characterised in that this Chrysosplenium nudicaule seed extract adopts pharmaceutical methods conventional in pharmacy to prepare into oral formulations.
Described oral formulations is preferably prepared for tablet, pill, hard capsule, granule, oral liquid.
Described Chrysosplenium nudicaule seed extract can be prepared as follows as hard capsule: takes Chrysosplenium nudicaule seed extract, is ground into fine powder, adds adjuvant, mixing, loads hard capsule, to obtain final product.
Described Chrysosplenium nudicaule seed extract can be prepared as follows as tablet: takes Chrysosplenium nudicaule seed extract, is ground into fine powder, adds adjuvant, mixing, makes granule, dry, and tabletted to obtain final product.
Chrysosplenium nudicaule seed extract of the present invention can be used at preparation treatment hyperlipidemia medicine or health product.
Chrysosplenium nudicaule seed extract of the present invention can be additionally used at preparation treatment vascular hypertension medicine or health product.
The technique effect of the present invention is verified by following experimentation:
Experiment one: the experimentation of Chrysosplenium nudicaule seed extract effect for reducing blood fat
1 experiment material
1.1 medicine Chrysosplenium nudicaule seed extracts (prepared by the method described in description embodiment 1 of the present invention)；XUEZHIKANG JIAONANG (Beijing WBL Peking University Biotech Co., Ltd).
1.2 reagent triglyceride determination test kits, T-CHOL measures test kit, low-density LP determination reagent box, high-density LP determination reagent box.
1.3 animal Kunming mouses, male and female half and half, body weight 18～22g.
1.4 instrument B-260 type thermostat water baths, TDL80-2B type low speed centrifuge, DG5033A type enzyme-linked immunosorbent assay instrument.
2 experimental techniques
The preparation of 2.1 test samples
2.1.1 the medicinal liquid of high dose group is prepared precision and is weighed Chrysosplenium nudicaule seed extract sample 3g, and adding 100mL concentration is 0.5%CMC-Na, is configured to the medicinal liquid that concentration is 0.03g/mL, standby.
2.1.2 the medicinal liquid of low dose group is prepared precision and is weighed Chrysosplenium nudicaule seed extract sample 1.5g, ibid method, is configured to the medicinal liquid that concentration is 0.015g/mL, standby.
2.1.3 the medicinal liquid of positive controls is prepared precision and is weighed Xuezhikang content 5g, ibid method, is configured to the medicinal liquid that concentration is 0.05g/mL, standby.
Egg yolk liquid 75mL is drawn in the preparation of 2.2 egg-nog solution, puts in the graduated cylinder of 100mL, with normal saline dilution to 100mL, is made into the egg-nog homogeneous solution of 75%.
2.3 packets take 50 Kunming mouses with administration, male and female half and half, body weight is within the scope of 18～22g, it is randomly divided into 5 groups, often group 10, it is respectively as follows: Normal group, hyperlipidemia model group, positive controls, high dose Chrysosplenium nudicaule seed extract administration group and low dosage Chrysosplenium nudicaule seed extract administration group, gastric infusion.It is 0.5%CMC-Na solution that Normal group and hyperlipidemia model group give concentration every day, and giving volume is 0.2mL/10g；Positive controls, Chrysosplenium nudicaule seed extract high dose administration group, Chrysosplenium nudicaule seed extract low dosage administration group gives relative medicine respectively, and dosage is 1g/kg, 0.6g/kg and 0.3g/kg respectively.Being administered 14 days, last two hours after administration, except Normal group, all the other respectively organize equal lumbar injection 75% egg-nog solution 0.02mL/g modeling, after modeling 20 hours, take blood from eyeball, by blood sample in 3000rpm min-1Centrifugal 10min, separation obtains serum, operates according to T-CHOL, triglyceride, low density lipoprotein, LDL and high density lipoprotein test kit description, measures the concentration of T-CHOL (TC), triglyceride (TG), low density lipoprotein, LDL (LDL-C) and high density lipoprotein (HDL-C).
2.4 statistical procedures the data obtained SPSS10.0 statistical softwares carry out statistical analysis, analyze result with± s represents, statistically significant with P < 0.05.
3 experimental results
After various dose Chrysosplenium nudicaule seed extract is administered 14 days, measuring the concentration of the T-CHOL (TC) in each group of experiment mice serum, triglyceride (TG), low density lipoprotein, LDL (LDL-C) and high density lipoprotein (HDL-C) respectively, experiment and analysis result are in Table 1.
Table 1 various dose Chrysosplenium nudicaule seed extract administration group on the impact of the corresponding blood lipids index of mice (± s)
Note: compare with Normal group, ##P < 0.01；Compare with hyperlipidemia model group, * * P < 0.01, * P < 0.05
Experimental result shows, the water average specific Normal group of TC, TG, LDL-C of hyperlipidemia model group significantly raises (P < 0.01), and the level of HDL-C decreases than Normal group (P < 0.01), it was shown that induced Hyperlipidemia in Mice model modeling success；Compared with model group, Chrysosplenium nudicaule seed extract high and low dose administration group all can significantly reduce TC, TG, LDL-C level (P < 0.01) of mice, can raise the level (P < 0.05) of HDL-C, and effect of high dosage becomes apparent from.
4 conclusions
This experimentation shows that the reduction of TC, TG, LDL-C is had obvious effect by Chrysosplenium nudicaule seed extract, HDL-C is had obvious rising effect, Chrysosplenium nudicaule seed extract has the definite effect reducing laboratory animal blood fat, and it has the exploitation prospect for blood lipid-lowering medicine.
Research is it is also shown that the Chrysosplenium nudicaule seed extract of the present invention also has good hypotensive effect, it is possible to treatment vascular hypertension.
Detailed description of the invention:
Embodiment 1:
(1) Chrysasplenium nudicaule is pulverized 20kg, put in baking oven 45 DEG C dry 4h after, add 20 times amount 80% acetone, in 65 DEG C of water-bath reflux, extract, 3 times, each 75min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 45 DEG C of water bath condition, filtering, filtrate carries out chromatography in 45 DEG C of water-baths after concentrating under reduced pressure；
(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:8, loading volume 7BV, washing 5BV remove impurity, with ethyl acetate: the eluant 8BV eluting of acetone=100:1, flow velocity is 3BV/h, collect eluent, eluent concentrates, dry, obtains Chrysosplenium nudicaule seed extract 10.4g.
Embodiment 2:
(1) Chrysasplenium nudicaule is pulverized 20kg, put in baking oven 40 DEG C dry 5h after, add 15 times amount 90% acetone, in 60 DEG C of water-bath reflux, extract, 4 times, each 70min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 50 DEG C of water bath condition, filtering, filtrate carries out chromatography in 50 DEG C of water-baths after concentrating under reduced pressure；
(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7, loading volume 8BV, washing 4BV remove impurity, with ethyl acetate: the eluant 9BV eluting of acetone=100:1, flow velocity is 2BV/h, collect eluent, eluent concentrates, dry, obtains Chrysosplenium nudicaule seed extract 10.2g.
Embodiment 3:
(1) Chrysasplenium nudicaule is pulverized 20kg, put in baking oven 50 DEG C dry 3h after, add 25 times amount 70% acetone, in 70 DEG C of water-bath reflux, extract, 2 times, each 80min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 40 DEG C of water bath condition, filtering, filtrate carries out chromatography in 40 DEG C of water-baths after concentrating under reduced pressure；
(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:9, loading volume 6BV, washing 6BV remove impurity, with ethyl acetate: the eluant 7BV eluting of acetone=100:1, flow velocity is 4BV/h, collect eluent, eluent concentrates, dry, obtains Chrysosplenium nudicaule seed extract 9.8g.
Embodiment 4: tablet
Take Chrysosplenium nudicaule seed extract, add appropriate amount of starch, mixing, make granule, dry, tabletted, coating, to obtain final product.
Embodiment 5: tablet
Take Chrysosplenium nudicaule seed extract, add appropriate dextrin, mixing, tabletting, to obtain final product.
Embodiment 6: hard capsule
Take Chrysosplenium nudicaule seed extract, add appropriate dextrin, mixing, make granule, load capsule, make hard capsule, to obtain final product.
Claims (9)
1. a Chrysosplenium nudicaule seed extract with effect for reducing blood fat, it is characterised in that this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 40 DEG C～50 DEG C dry 3h～5h after, add 15～25 times amount 70%～90% acetone, in 60 DEG C～70 DEG C water-bath reflux, extract, 2 times～4 times, each 70min～80min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 40 DEG C～50 DEG C water bath condition, filtering, filtrate carries out chromatography after concentrating under reduced pressure in 40 DEG C～50 DEG C water-baths；
(3) AB-8 type macroporous adsorbent resin is selected to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7～9, loading volume 6BV～8BV, washing 4BV～6BV remove impurity, with ethyl acetate: the eluant 7BV～9BV eluting of acetone=100:1, flow velocity is 2BV/h～4BV/h, collect eluent, eluent concentrates, dry, to obtain final product.
2. Chrysosplenium nudicaule seed extract as claimed in claim 1, it is characterised in that this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 40 DEG C dry 5h after, add 15 times amount 90% acetone, in 60 DEG C of water-bath reflux, extract, 4 times, each 70min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 50 DEG C of water bath condition, filtering, filtrate carries out chromatography in 50 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:7, loading volume 8BV, washes 4BV remove impurity, with ethyl acetate: the eluant 9BV eluting of acetone=100:1, flow velocity is 2BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
3. Chrysosplenium nudicaule seed extract as claimed in claim 1, it is characterised in that this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 50 DEG C dry 3h after, add 25 times amount 70% acetone, in 70 DEG C of water-bath reflux, extract, 2 times, each 80min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 40 DEG C of water bath condition, filtering, filtrate carries out chromatography in 40 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:9, loading volume 6BV, washes 6BV remove impurity, with ethyl acetate: the eluant 7BV eluting of acetone=100:1, flow velocity is 4BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
4. Chrysosplenium nudicaule seed extract as claimed in claim 1, it is characterised in that this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method:
(1) Chrysasplenium nudicaule is pulverized, put in baking oven 45 DEG C dry 4h after, add 20 times amount 80% acetone, in 65 DEG C of water-bath reflux, extract, 3 times, each 75min, united extraction liquid, reclaim acetone, obtain Chrysasplenium nudicaule total extract extractum；
(2) by the Chrysasplenium nudicaule total extract extractum that obtains with chloroform: the mixed solution of methanol=50:1 is stirring and dissolving under 45 DEG C of water bath condition, filtering, filtrate carries out chromatography in 45 DEG C of water-baths after concentrating under reduced pressure；
(3) selecting AB-8 type macroporous adsorbent resin to carry out column chromatography for separation, resin column blade diameter length ratio is 1:8, loading volume 7BV, washes 5BV remove impurity, with ethyl acetate: the eluant 8BV eluting of acetone=100:1, flow velocity is 3BV/h, collects eluent, eluent concentrates, dry, to obtain final product.
5. the Chrysosplenium nudicaule seed extract as described in Claims 1 to 4 any one, it is characterised in that this Chrysosplenium nudicaule seed extract adopts pharmaceutical methods conventional in pharmacy to prepare into oral formulations.
6. Chrysosplenium nudicaule seed extract as claimed in claim 5, it is characterised in that this Chrysosplenium nudicaule seed extract adopts pharmaceutical methods conventional in pharmacy to prepare into tablet, pill, hard capsule, granule, oral liquid.
7. Chrysosplenium nudicaule seed extract as claimed in claim 6, it is characterised in that the hard capsule of this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method: take Chrysosplenium nudicaule seed extract, is ground into fine powder, adds adjuvant, mixing, loads hard capsule, to obtain final product.
8. Chrysosplenium nudicaule seed extract as claimed in claim 6, it is characterised in that the tablet of this Chrysosplenium nudicaule seed extract is adopted and prepared with the following method: takes Chrysosplenium nudicaule seed extract, is ground into fine powder, add adjuvant, mixing, make granule, dry, tabletted, to obtain final product.
9. the application in preparation treatment hyperlipidemia medicine or health product of the Chrysosplenium nudicaule seed extract as described in Claims 1 to 4 any one.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103585206A (en) * | 2013-10-31 | 2014-02-19 | 济南星懿医药技术有限公司 | Preparation method of Chrysosplenium extract and its application |
| CN103585205A (en) * | 2013-10-31 | 2014-02-19 | 济南星懿医药技术有限公司 | Application of Chrysosplenium extract in preparation of drugs treating diabetes |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103585206A (en) * | 2013-10-31 | 2014-02-19 | 济南星懿医药技术有限公司 | Preparation method of Chrysosplenium extract and its application |
| CN103585205A (en) * | 2013-10-31 | 2014-02-19 | 济南星懿医药技术有限公司 | Application of Chrysosplenium extract in preparation of drugs treating diabetes |
Non-Patent Citations (1)
| Title |
|---|
| 藏药金腰草中黄酮诱导K562细胞凋亡及其分子机制研究;王玉平等;《实用癌症杂志》;20050731;第20卷(第4期);374-376 * |
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