CN103563887B - Culture medium formula for animal sperm in-vitro normal temperature preservation and transportation and production technology thereof - Google Patents
Culture medium formula for animal sperm in-vitro normal temperature preservation and transportation and production technology thereof Download PDFInfo
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- CN103563887B CN103563887B CN201310479937.7A CN201310479937A CN103563887B CN 103563887 B CN103563887 B CN 103563887B CN 201310479937 A CN201310479937 A CN 201310479937A CN 103563887 B CN103563887 B CN 103563887B
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Abstract
The invention discloses a culture medium formula for animal sperm in-vitro normal temperature preservation and transportation. The culture medium formula comprises the following components in parts by weight: 30-40 parts of anhydrous dextrose, 3-10 parts of sodium citrate, 1-2 parts of ethylenediamine tetraacetic acid disodium salt, 0.5-2 parts of sodium bicarbonate, 1-2 parts of sodium chloride and 0.5-2 parts of incomycin hydrochloride. The culture medium is prepared by using a preparation device of the culture medium for animal sperm in-vitro normal temperature preservation and transportation through mixing. The culture medium formula for animal sperm in-vitro normal temperature preservation and transportation is capable of prolonging the in-vitro preservation and transportation time and the survival rate of animal sperms and ensuring the power of animal sperm life extension.
Description
Technical field
The present invention relates to animal sperm preservation technology field, be specifically related to the external normal temperature of a kind of animal sperm and preserve transport medium formula and production technology thereof.
Background technology
In livestock breeding, animal mating all adopts natural mating mode mostly, and this mode then defect is that feed cost is high, and natural mating transmission risk is high.In order to avoid these problems, livestock breeding field starts to adopt the mode of external artificial insemination to breed, and replaces animal natural mating, reduces transmission, expanding propagation ability.And to carry out the most important link of artificial insemination be exactly for the time-to-live of animal sperm in animal culture in vitro, animal sperm is longer at animal survival time in vitro, is more conducive to improving animal breeding capacity, improves premium animal gene extension ability.Usually containing having an appointment 10 in the fresh essence of animal
4~ 10
5individual/ml bacterium, great majority are G
-bacterium, these bacteriums may affect the semen at normal temperature holding time, and much virus finds or reports to infertile or to reduce fertility relevant in infected pig semen.The animal semen of artificial collection wants to preserve, transport and semen deposition, must add suitable sperm in vitro and survive and the nutrient solution keeping sperm capacitation in the seminal fluid gathered.In the process of cryopreservation of sperm, if preserve improper, the vigor of sperm, membrane integrity and acrosomal integnity can be affected.
The external normal temperature of current exploitation animal sperm preserves transport medium, reduces production cost further, improves the quality of products, and improve animal improved seeds fertility, reducing production cost is manufacturer's problem demanding prompt solution.Current some relevant products that occurred, but these products and production technology are difficult to reach quantification production, can not active and effective raising animal improved seeds fertility.
Summary of the invention
For overcoming the defect of prior art, of the present invention being provides the external normal temperature of a kind of animal sperm to preserve transport medium formula, and the time of prolongation animal sperm storage in vitro, transport and survival rate, ensure the power of animal sperm life continuation.
Another object of the present invention is to provide the external normal temperature of a kind of animal sperm to preserve transport medium to fill a prescription its production technology, realize suitability for industrialized production, improve product quality and production efficiency.
The technical solution adopted in the present invention is as follows for achieving the above object:
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 30-40 part
Sodium citrate 3-10 part
Disodium ethylene diamine tetraacetate 1-2 part
Sodium bicarbonate 0.5-2 part
Sodium chloride 1-2 part
Lincomycin Hydrochloride 0.5-2 part.
In preferred scheme, the external normal temperature of described animal sperm is preserved transport medium formula and is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 34.5-36.5 part
Sodium citrate 4-6 part
Disodium ethylene diamine tetraacetate 1-1.5 part
Sodium bicarbonate 1-1.5 part
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1-1.5 part.
Further in preferred scheme, the external normal temperature of described animal sperm is preserved transport medium formula and is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 35 parts
Sodium citrate 5 parts
Disodium ethylene diamine tetraacetate 1.5 parts
Sodium bicarbonate 1 part
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1 part.
The external normal temperature of animal sperm preserves a production technology for transport medium formula, and its step is as follows:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, above-mentioned raw materials mixes by High Rotation Speed mixing tank;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue High Rotation Speed mixing tank, mix.
In said method, the rotating speed of described High Rotation Speed is 180-250r/min.
As the preferred scheme of one of the present invention, in said method, the time of the mixing tank High Rotation Speed described in step 1) is 15-25 minute.
As the preferred scheme of one of the present invention, in said method, step 2) described in time of mixing tank High Rotation Speed be 25-35 minute.
In such scheme, the external normal temperature of animal sperm of the present invention preserves device disclosed in the utility model patent (application number is CN201220712455.2) applied for before transport medium preparing device is the application.Its concrete structure is as follows.
The external normal temperature of a kind of animal sperm preserves transport medium preparing device, and it comprises stainless steel " V " type mixing tank, base and transmission mechanism; The top of described stainless steel " V " type mixing tank is respectively equipped with charging aperture and vacuum extractor, is provided with discharging opening bottom it; Described base comprises the first support and the second support, one rotating shaft is through the bucket chamber of stainless steel " V " type mixing tank, its two ends are rotatably connected on the upper end of the first support and the second support respectively, one end connection for transmission mechanism of rotating shaft, transmission mechanism drives axis of rotation, the rotating shaft being positioned at barrel chamber is provided with some stirring pieces, and the bucket cavity wall of described stainless steel " V " type mixing tank extends some ganoid protrusions by inwall, and described protrusion is hemispherical or strip; Described section that is hemispherical or strip protrusion is semicircle; Described transmission mechanism comprises the drive link that electromotor is connected with electromotor with one end, and the other end of drive link is connected with rotating shaft; It also comprises sampler, and sampler comprises body, push rod and outlet, and the bucket wall that described body one end is welded on stainless steel " V " type mixing tank communicates with bucket chamber, and its other end is blind end; Push rod is positioned at body, its front end arranges a baffle plate and gos deep in bucket chamber, the mouth of pipe of baffle plate lucky energy closed tube and junction, bucket chamber, the top that push rod stretches out the part of body is provided with end cap, one spring housing stretches out in the part of body at push rod, on the blind end that the two ends of spring are separately fixed at body and on end cap, outlet is positioned at the bottom of body.
Beneficial effect of the present invention is compared to existing technology:
1. the of the present invention kind of external normal temperature of animal sperm preserves transport medium formula to contain pharmaceutical grade DEXTROSE ANHYDROUS, food grade sodium citrate for primary raw material, wherein active ingredient DEXTROSE ANHYDROUS can provide life continuation energy for animal sperm, departs from the nutrition outside animal body and defencive function;
2. to preserve transport medium formulation stability strong for the external normal temperature of of the present invention kind of animal sperm, watch for animals sperm in vitro survive for days still possess the fertilizing ability of more than 95%;
3. the external normal temperature of of the present invention kind of animal sperm is preserved transport medium formula and can be extended Boar spermatozoa and survive in vitro 3-5 days and do not lose fertilizing ability.
Below in conjunction with concrete embodiment, the present invention is described in further detail.
Embodiment
It is below specific embodiment of the present invention.
Embodiment 1
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 30 parts
Sodium citrate 10 parts
Disodium ethylene diamine tetraacetate 2 parts
Sodium bicarbonate 2 parts
1 part, sodium chloride
Lincomycin Hydrochloride 0.5 part; And be prepared from through following methods:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, rotate mixing tank with the rotating speed of 180r/min above-mentioned raw materials is mixed;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue to rotate mixing tank with the rotating speed of 180r/min, mix.
Embodiment 2
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 40 parts
Sodium citrate 3 parts
Disodium ethylene diamine tetraacetate 1 part
Sodium bicarbonate 2 parts
2 parts, sodium chloride
Lincomycin Hydrochloride 0.5-2 part; And be prepared from through following methods:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, rotate mixing tank with the rotating speed of 200r/min above-mentioned raw materials is mixed;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue to rotate mixing tank with the rotating speed of 200r/min, mix.
Embodiment 3
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 35 parts
Sodium citrate 5 parts
Disodium ethylene diamine tetraacetate 1.5 parts
Sodium bicarbonate 1 gram of part
Sodium chloride 1.5 grams of parts
Lincomycin Hydrochloride 1 part; And be prepared from through following methods:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, rotate mixing tank with the rotating speed of 250r/min above-mentioned raw materials is mixed;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue to rotate mixing tank with the rotating speed of 250r/min, mix.
Embodiment 4
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 34.5 parts
Sodium citrate 6 parts
Disodium ethylene diamine tetraacetate 1.5 parts
Sodium bicarbonate 1 part
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1.5 parts; And be prepared from through following methods:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, rotate mixing tank with the rotating speed of 200r/min above-mentioned raw materials is mixed;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue to rotate mixing tank with the rotating speed of 200r/min, mix.
Embodiment 5
The external normal temperature of a kind of animal sperm preserves transport medium formula, and it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 36.5 parts
Sodium citrate 4 parts
Disodium ethylene diamine tetraacetate 1 part
Sodium bicarbonate 1.5 parts
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1 part; And be prepared from through following methods:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, rotate mixing tank with the rotating speed of 200r/min above-mentioned raw materials is mixed;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue to rotate mixing tank with the rotating speed of 200r/min, mix.
Performance Detection
To physical behavior, density, acid number, acidity, heavy metal, the lead of the product in above-described embodiment 1-5, female, water is not tolerant, moisture, PH, granularity, color and luster lamp behaviour measure according to the method in GB/6782-2009, GB/T640-1997, and measurement result is in table 1.
Table 1
According to the result of table 1, the product in embodiment of the present invention 1-5 is high-class product.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.
Claims (7)
1. the external normal temperature of animal sperm preserves a transport medium formula, it is characterized in that it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 30-40 part
Sodium citrate 3-10 part
Disodium ethylene diamine tetraacetate 1-2 part
Sodium bicarbonate 0.5-2 part
Sodium chloride 1-2 part
Lincomycin Hydrochloride 0.5-2 part.
2. the external normal temperature of animal sperm according to claim 1 preserves transport medium formula, it is characterized in that it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 34.5-36.5 part
Sodium citrate 4-6 part
Disodium ethylene diamine tetraacetate 1-1.5 part
Sodium bicarbonate 1-1.5 part
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1-1.5 part.
3. the external normal temperature of animal sperm according to claim 1 preserves transport medium formula, it is characterized in that it is made up of the following component with parts by weight:
DEXTROSE ANHYDROUS 35 parts
Sodium citrate 5 parts
Disodium ethylene diamine tetraacetate 1.5 parts
Sodium bicarbonate 1 part
1.5 parts, sodium chloride
Lincomycin Hydrochloride 1 part.
4. the external normal temperature of animal sperm as claimed in claim 1 preserves a production technology for transport medium formula, it is characterized in that its step is as follows:
1) according to formula ratio, sodium citrate, disodium ethylene diamine tetraacetate, sodium bicarbonate, sodium chloride, Lincomycin Hydrochloride are loaded the external normal temperature of animal sperm and preserve in the mixing tank of transport medium preparing device, under the drive of transmission mechanism, above-mentioned raw materials mixes by High Rotation Speed mixing tank;
2) in the mixed raw material of step 1), add DEXTROSE ANHYDROUS, continue High Rotation Speed mixing tank, mix.
5. the external normal temperature of animal sperm according to claim 4 preserves the production technology of transport medium formula, it is characterized in that: the rotating speed of described High Rotation Speed is 180-250r/min.
6. the external normal temperature of animal sperm according to claim 5 preserves the production technology of transport medium formula, and it is characterized in that: in step 1), the time of mixing tank High Rotation Speed is 15-25 minute.
7. the external normal temperature of animal sperm according to claim 5 preserves the production technology of transport medium formula, it is characterized in that: step 2) in, the time of mixing tank High Rotation Speed is 25-35 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310479937.7A CN103563887B (en) | 2013-10-14 | 2013-10-14 | Culture medium formula for animal sperm in-vitro normal temperature preservation and transportation and production technology thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310479937.7A CN103563887B (en) | 2013-10-14 | 2013-10-14 | Culture medium formula for animal sperm in-vitro normal temperature preservation and transportation and production technology thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103563887A CN103563887A (en) | 2014-02-12 |
| CN103563887B true CN103563887B (en) | 2015-05-06 |
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|---|---|---|---|
| CN201310479937.7A Expired - Fee Related CN103563887B (en) | 2013-10-14 | 2013-10-14 | Culture medium formula for animal sperm in-vitro normal temperature preservation and transportation and production technology thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349797A (en) * | 2000-09-11 | 2002-05-22 | Imv技术公司 | Attenuant for preserving pig's sperm |
| CN101613677A (en) * | 2009-07-30 | 2009-12-30 | 广西大学 | A kind of method of separating pig X sperm and y sperm |
| CN203018017U (en) * | 2012-12-20 | 2013-06-26 | 杨辉文 | Preparation device for animal sperms in-vitro normal temperature preservation and transportation culture medium |
-
2013
- 2013-10-14 CN CN201310479937.7A patent/CN103563887B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349797A (en) * | 2000-09-11 | 2002-05-22 | Imv技术公司 | Attenuant for preserving pig's sperm |
| CN101613677A (en) * | 2009-07-30 | 2009-12-30 | 广西大学 | A kind of method of separating pig X sperm and y sperm |
| CN203018017U (en) * | 2012-12-20 | 2013-06-26 | 杨辉文 | Preparation device for animal sperms in-vitro normal temperature preservation and transportation culture medium |
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| CN103563887A (en) | 2014-02-12 |
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