CN102178080A - Feeding method capable of promoting growth and increasing immunity and stress resistance of Cyprinus carpio var. jian - Google Patents
Feeding method capable of promoting growth and increasing immunity and stress resistance of Cyprinus carpio var. jian Download PDFInfo
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- CN102178080A CN102178080A CN2011100905908A CN201110090590A CN102178080A CN 102178080 A CN102178080 A CN 102178080A CN 2011100905908 A CN2011100905908 A CN 2011100905908A CN 201110090590 A CN201110090590 A CN 201110090590A CN 102178080 A CN102178080 A CN 102178080A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a feeding method capable of promoting growth and increasing immunity and stress resistance of Cyprinus carpio var. jian, which is to feed the Cyprinus carpio var. jian with a compound feed (A) for Cyprinus carpio var. jian and a compound feed (B) for Cyprinus carpio var. jian alternately. Many experiments are carried out, and the result of the experiments prove that the feeding method can promote the growth of Cyprinus carpio var. jian and the utilization of feed, improve the protein content in muscle, improve the deposition of protein for promoting growth and improve the antioxidizing capacity and immunity of the Cyprinus carpio var. jian; and after being squeezed and stressed, the Cyprinus carpio var. jian can relieve the influence of stress by changing blood sugar, cortisol and serum heat shock protein (HSP)70. The feeding method disclosed by the invention increases the growth speed of the Cyprinus carpio var. jian, disease resistance and stress resistance.
Description
Technical field
The invention belongs to aquaculture field, exactly is the method for breeding that promotes the jian carp growth, strengthens immunity and anti-stress ability.
Background technology
Jian carp is the more stable good fish culture new varieties of the 1st inhereditary feature of China that China Aquatic Science Research Academy Fresh Water Fishery Research Center manually breeds.Select red carp of maternal pocket and male parent Yuanjiang River carp, filial generation is as the breeding underlying group.The jian carp fast growth, the long build of jian carp, cinerous, the meat meat flavour is good, the feed conversion rate height, warm in nature suitable, easily to raise and train, easily rise and catch, adaptability and premunition are strong, being fit to multiple mode in all parts of the country cultures, obviously be better than domestic existing carp and external introduced variety, account for 50% of national carp culture total output, become one of topmost carp culture kind of China.
Jian carp promoted for two more than ten years in China, and along with continuous progress in science and technology, the research of jian carp feed is gradually improved, and did not almost have breakthrough in recent years, had entered bottleneck stage.
Summary of the invention
The mixed feed that the purpose of this invention is to provide a kind of jian carp.
Jian carp mixed feed A, its prescription composition by weight/%:
Domestic fish meal 46.5～52.5, glutamine dipeptide (Ala-Gln) 0.45～0.65, alanine 0.30～0.40, fish oil 1.25～1.50, corn oil 1.35～1.55, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.50～7.50, inferior powder 28.50～35.50, swelling scholar 2.35～2.65, alphalise starch 1.0;
Be preferably:
Domestic fish meal 48.50, glutamine dipeptide (Ala-Gln) 0.55, alanine 0.36, fish oil 1.42, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.74, inferior powder 33.58, bentonite 2.65, alphalise starch 1.0;
Or domestic fish meal 52.50, glutamine dipeptide (Ala-Gln) 0.45, alanine 0.35, fish oil 1.25, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, dextrin 7.50, flour 29.40, cellulose 2.35, adhesive 1.0;
Or on the domestic fish meal 46.5, glutamine dipeptide (Ala-Gln) 0.65, alanine 0.40, fish oil 1.50, corn oil 1.55, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.50, inferior powder 35.50, swelling 2.65, alphalise starch 1.0;
Described vitamin premix (mg/kg feed): thiamine hydrochloride, 64; Riboflavin, 195; Folic acid, 18; Puridoxine hydrochloride, 45; Niacin, 820; Calcium pantothenate, 285; Inositol, 418; Biotin, 6.5; Vit E, 420; Vit B
12, 0.15; Vit A, 1.5; Vit D
3, 0.06; Vit C, 285;
Described inorganic salts premix (g or mg/kg feed): epsom salt, 1.8g; Ironic citrate, 0.9g; White vitriol, 0.5g; Cupric sulfate pentahydrate, 5mg; Six water alchlors, 2.5mg; KI, 2.8mg; Sodium selenite, 0.15mg; Sulfuric acid monohydrate is violent, 3.8mg; CoCL2,1.9mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g;
Jian carp mixed feed (B), its prescription composition by weight/%:
Domestic fish meal 51.50, alanine 0.90, fish oil 1.44, corn oil 1.43, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.8, calcium dihydrogen phosphate 0.50, wheat bran 6.25, inferior powder 31.45, bentonite 2.18, alphalise starch 1.1.
Described vitamin premix (mg/kg feed): thiamine hydrochloride, 65; Riboflavin, 205; Folic acid, 15; Puridoxine hydrochloride, 40; Niacin, 800; Calcium pantothenate, 280; Inositol, 400; Biotin, 5; Vit E, 415; Vit B
12, 0.11; VitA, 1.3; Vit D
3, 0.05; Vit C, 200.
Described inorganic salts premix (g or mg/kg feed): epsom salt, 1.6g; Ironic citrate, 0.8g; White vitriol, 0.4g; Cupric sulfate pentahydrate, 6mg; Six water alchlors, 3mg; KI, 3mg; Sodium selenite, 0.2mg; Sulfuric acid monohydrate is violent, 4mg; CoCL2,2mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g.
Another object of the present invention is the method for breeding that promotes the jian carp growth, strengthens immunity and anti-stress ability, and it comprises:
1) artificial incubation one age the carp fingerling, the jian carp mixed feed B 15 days of throwing something and feeding is satiated with food;
2) throw something and feed jian carp mixed feed (A) 1-4 after week, throw something and feed again jian carp mixed feed (B) 1-4 week;
3) set by step 2) rule continues alternately to throw something and feed jian carp mixed feed (A), (B);
After being preferably 2 weeks of jian carp mixed feed (A) of throwing something and feeding, feeding method is alternately thrown at the interval in 2 weeks of jian carp mixed feed (B) of throwing something and feeding again.
The method for breeding of promotion jian carp provided by the invention growth, enhancing immunity and anti-stress ability, adopt jian carp mixed feed (A) and jian carp mixed feed (B) alternately to throw at interval and raise jian carp, and carried out multinomial experiment, experimental result shows, method for breeding of the present invention can promote growth, the feed conversion of jian carp, also improves Protein content in the muscle simultaneously, promote the deposition of protein and improve growth, improved oxidation resistance and the immunity of jian carp; After crowded the coercing, jian carp can be alleviated by the variation of blood sugar, blood cortisol and serum HSP70 and coerces the influence that causes.Method for breeding of the present invention has improved the speed of growth of jian carp, has strengthened premunition and the stress ability of jian carp.
The specific embodiment
Embodiment 1 jian carp mixed feed (A), its prescription composition by weight/%:
Domestic fish meal 48.50, glutamine dipeptide (Ala-Gln) 0.55, alanine 0.36, fish oil 1.42, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.74, inferior powder 33.58, bentonite 2.65, alphalise starch 1.0.
Feedstuff weighs, adopts the method that enlarges step by step evenly to mix through pulverizing 60 mesh sieves by prescription, and being processed into diameter is the 1.0mm particle, kept dry.
Vitamin premix (mg/kg feed): thiamine hydrochloride, 64; Riboflavin, 195; Folic acid, 18; Puridoxine hydrochloride, 45; Niacin, 820; Calcium pantothenate, 285; Inositol, 418; Biotin, 6.5; Vit E, 420; Vit B
12, 0.15; VitA, 1.5; Vit D
3, 0.06; Vit C, 285.
Inorganic salts premix (g or mg/kg feed): epsom salt, 1.8g; Ironic citrate, 0.9g; White vitriol, 0.5g; Cupric sulfate pentahydrate, 5mg; Six water alchlors, 2.5mg; KI, 2.8mg; Sodium selenite, 0.15mg; Sulfuric acid monohydrate is violent, 3.8mg; CoCL2,1.9mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g.
Embodiment 2 jian carp mixed feeds (A), its prescription composition by weight/%:
Domestic fish meal 52.50, glutamine dipeptide (Ala-Gln) 0.45, alanine 0.35, fish oil 1.25, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 7.50, inferior powder 29.40, bentonite 2.35, alphalise starch 1.0.
Embodiment 3 jian carp mixed feeds (A), its prescription composition by weight/%:
Domestic fish meal 46.5, glutamine dipeptide (Ala-Gln) 0.65, alanine 0.40, fish oil 1.50, corn oil 1.55, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.50, inferior powder 35.50, bentonite 2.65, alphalise starch 1.0.
Embodiment 4 jian carp mixed feeds (B), its prescription composition by weight/%:
Domestic fish meal 51.50, alanine 0.90, fish oil 1.44, corn oil 1.43, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.8, calcium dihydrogen phosphate 0.50, wheat bran 6.25, inferior powder 31.45, bentonite 2.18, alphalise starch 1.1;
Vitamin premix (mg/kg feed): thiamine hydrochloride, 65; Riboflavin, 205; Folic acid, 15; Puridoxine hydrochloride, 40; Niacin, 800; Calcium pantothenate, 280; Inositol, 400; Biotin, 5; Vit E, 415; Vit B
12, 0.11; VitA, 1.3; Vit D
3, 0.05; Vit C, 200.
Inorganic salts premix (g or mg/kg feed): epsom salt, 1.6g; Ironic citrate, 0.8g; White vitriol, 0.4g; Cupric sulfate pentahydrate, 6mg; Six water alchlors, 3mg; KI, 3mg; Sodium selenite, 0.2mg; Sulfuric acid monohydrate is violent, 4mg; CoCL2,2mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g.
Above-mentioned raw materials through pulverizing 60 mesh sieves, is mixed, and being processed into diameter is the 1.0mm particle, kept dry.
Embodiment 5
The test fish from a collection of artificial incubation one age the carp fingerling.Support temporarily in indoor aquarium before the test, select stalwartness, uniform juvenile fish 1000 tails of specification; Put in the indoor aquarium and tame, the jian carp mixed feed (B) of the throwing something and feeding 15d that throws something and feeds that is satiated with food; Feeding experiment schedules to last 8w; According to 5 processed group of experimental design (seeing Table 1), every group of 3 repetitions, before on-test, stop the 1d that throws something and feeds, select then physically fit, the uniform carp of specification (33.52 ± 0.17g), put in a suitable place to breed at random in indoor aquarium, cultivation density is 20g/L, day rate of throwing something and feeding is the 2.5-4.0% of body weight, looks fish body size, water temperature and the situation of ingesting and does suitably to adjust.Throwing something and feeding every day, twice (9:00,17:00), collecting residual bait, weigh in the back of throwing something and feeding, calculates food ration.The duration of test water temperature is 23-30 ℃, and pH is 7.1 ± 0.1, and dissolved oxygen is greater than 5mgL
-1, ammonia nitrogen is less than 0.4mgL
-1
Table 1 experimental design
Embodiment 6
Raise 8w, the 24h that stops eating before the sampling, each repeats to weigh respectively.Calculate specific growth rate (SGR), feed grazing rate (FI) and feed efficiency etc.Get 9 tail fishes (every repetition 3 tail fishes) in addition for every group, blot the moisture of fish body surface after, get the white muscle that side line is above, dorsal fin is following, measure its nutritional labeling.The body weight and the body of every group of each replication 10 tail are long, calculate coefficient of condition.Specifically be calculated as follows.
Specific growth rate (%d
-1)=(lnW
t-lnW
0) * 100/t
Feed coefficient=I/ (W
t-W
0)
Feed grazing rate (%d
-1)=100 * I/[W
0+ W
t]/2 * t
Protein efficiency ratio, PER (PER)=body weight gains/protein intake
Dirty body is than (VI)=internal organ quality (g) * 100/ fish physique amount (g)
Coefficient of condition (CF)=(W/L
3) * 100
I (g) is for taking in the dry weight of feed, W
0(g) and W
t(g) be the gross weight of first last fish body, t (d) is the fate of test, and it is long that W (g) and L (cm) are respectively body weight and the body of every tail fish.
Test data is represented with mean value ± standard error, adopts SPSS (16.0) software to carry out one-way analysis of variance, during significant difference, uses Duncan ' multiple ratio, P＜0.05 expression significant difference.It the results are shown in Table 2,3 and 4.
Table 2 jian carp mixed feed (A), (B) throw something and feed mode to the carp growth Effect on Performance
Annotate: indicate different lowercase persons in the table in the same column and represent group difference significantly (P＜0.05), following table 3-6 together.
Table 3 jian carp mixed feed (A), (B) influence of mode of throwing something and feeding to the carp morphological indexes
Table 4 jian carp mixed feed (A), (B) influence of mode of throwing something and feeding to the carp feed conversion
Embodiment 7
Raise 8w, the 24h that stops eating before the sampling gets fish 10 tails at random from every cylinder, gets blood with MS-222 (200mg/L) anesthesia from the tail vein, places 24h for 4 ℃, avoids vibration, prevents haemolysis, 4 ℃ of centrifuging and taking serum, and-20 ℃ of preservations are to be measured.The test fish of getting blood is dissected immediately, taken out liver pancreas, spleen and head-kidney ,-20 ℃ of preservations are to be measured.During mensuration, sample is thawed, liver pancreas, spleen and head-kidney respectively under condition of ice bath, homogenate in the glass homogenizer, homogenate 6000r/min, 4 ℃ of centrifugal 20min, supernatant make the mensuration that different indexs are carried out in suitably dilution.
The active mensuration of superoxide dismutase (SOD) adopts xanthine oxidase, and the SOD unit definition is that to reach 50% o'clock pairing SOD amount be a SOD unit of activity (U) to every milligram of histone SOD inhibiting rate in the 1mL reactant liquor.Two sulfo-dinitrobenzoic acid methods are measured serum glutathione peroxidase activity (glutathione peroxidase, GSH-Px), GSH-Px active unit is defined as every milligram of protein, the effect of per minute deduction non-enzyme reaction, making in the reaction system GSH concentration reduce by 1 μ g/L is 1 unit of activity.Turbidimetry is adopted in lysozyme (LZM) determination of activity.Nitric oxide (NO) adopts nitrate reductase method, and the Coomassie brilliant blue method is measured the protein content of tissue.Kit all builds up bio-engineering research institute from Nanjing.
It the results are shown in Table 5 and 6.
Table 5 jian carp mixed feed (A), (B) influence of mode of throwing something and feeding to SOD and the GSH-Px of carp
Table 6 jian carp mixed feed (A), (B) influence of mode of throwing something and feeding to LZM and the NO of carp
Tab 6Effect of feeding modes of Ala-Gln on LZM and NO of Cyprinus carpio
Embodiment 8
Acute crowded coercing when testing the culture experiment end respectively organized fish with low-density and transferred in the Plastic Drum that diameter is 50cm, and every barrel adds 10L water, and making its density is 110gL
-1The test fish is coerced 1h in the environment of crowded and restriction, be put back into then in the original cultivating system, and adjust to the cultivation density of 20g/L, do three repetitions equally, respectively before coercing beginning (contrast) and coerce afterwards 0,1,3,6,12,24,48,72h takes a sample, every group of each time period got 5 tail fishes, before the sampling, fish for juvenile fish at random fast, dropping into concentration immediately is 200mgL
-1MS.222 in do fast deep anesthesia, get blood with the Dispoable medical syringe from the tail vein, 4 ℃ leave standstill 24h after, the centrifugal 10min of 2500rpm, separation of serum ,-20 ℃ of preservations are standby.Stress during not bait throwing in, stress finish between convalescence normal bait throwing in.
Measure before and after anti-crowded the coercing anti-stress index adopt the glucose oxidase-peroxidase method to measure blood sugar, adopt liquid equilibrium competition radiommunoassay (RIA) method to measure serum cortisol, kit builds up biological study institute from Nanjing.Adopt enzyme-linked immune analytic method to measure heat shock protein-70 (HSP70) in the serum, kit is from the biological Co., Ltd of source, Shanghai leaf.The results are shown in Table 7,8 and 9.
Table 7 glutamine dipeptide is thrown something and fed mode to acute crowded influence of coercing carp blood sugar
Tab7Effect?of?feeding?modes?of?Ala-Gln?on?serum?glucose?of?Cyprinus?carpio?subjected?toacute?crowding?stress
Annotate: indicate different lowercase persons in the table among the colleague and represent group difference significantly (P＜0.05), following table 8-9 together.
Table 8 glutamine dipeptide is thrown something and fed mode to acute crowded influence of coercing the carp serum cortisol
Tab?8Effect?of?feeding?modes?of?Ala-Gln?on?serum?cortisol?of?Cyprinus?carpio?su?bjected?toacute?crowding?stress
Table 9 glutamine dipeptide is thrown something and fed mode to acute crowded influence of coercing carp serum HSP70
Tab9Effect?of?feeding?modes?of?Ala-Gln?on?serum?HSP70of?Cyprinus?carpio?subjected?toacute?crowding?stress
Show from above-mentioned experimental result, jian carp mixed feed (A) and jian carp mixed feed (B) were alternately thrown at interval and are raised jian carp in 2 weeks, can promote growth, the feed conversion of jian carp, simultaneously can improve Protein content in the muscle, promote the deposition of protein and improve growth, and oxidation resistance of jian carp and immunity; After crowded the coercing, jian carp can be alleviated by the variation of blood sugar, blood cortisol and serum HSP70 and coerces the influence that causes.Method for breeding of the present invention has improved the speed of growth of jian carp, has strengthened premunition and stress ability.
Claims (9)
1. jian carp mixed feed (A), its prescription composition by weight/%:
Domestic fish meal 46.5～52.5, glutamine dipeptide (Ala-Gln) 0.45～0.65, alanine 0.30～0.40, fish oil 1.25～1.50, corn oil 1.35～1.55, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.50～7.50, inferior powder 28.50～35.50, bentonite 2.35～2.65, alphalise starch 1.0.
2. jian carp mixed feed according to claim 1 (A) is characterized in that, the prescription composition by weight/%:
Domestic fish meal 48.50, glutamine dipeptide (Ala-Gln) 0.55, alanine 0.36, fish oil 1.42, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.74, inferior powder 33.58, bentonite 2.65, alphalise starch 1.0.
3. jian carp mixed feed according to claim 1 (A) is characterized in that, the prescription composition by weight/%:
Domestic fish meal 52.50, glutamine dipeptide (Ala-Gln) 0.45, alanine 0.35, fish oil 1.25, corn oil 1.45, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 7.50, inferior powder 29.40, bentonite 2.35, alphalise starch 1.0.
4. jian carp mixed feed according to claim 1 (A) is characterized in that, the prescription composition by weight/%:
Domestic fish meal 46.5, glutamine dipeptide (Ala-Gln) 0.65, alanine 0.40, fish oil 1.50, corn oil 1.55, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.80, calcium dihydrogen phosphate 0.50, wheat bran 6.50, inferior powder 35.50, bentonite 2.65, alphalise starch 1.0.
5. jian carp mixed feed according to claim 1 and 2 (A) is characterized in that:
Described vitamin premix (mg/kg feed): thiamine hydrochloride, 64; Riboflavin, 195; Folic acid, 18; Puridoxine hydrochloride, 45; Niacin, 820; Calcium pantothenate, 285; Inositol, 418; Biotin, 6.5; Vit E, 420; Vit B
12, 0.15; VitA, 1.5; Vit D
3, 0.06; Vit C, 285; Described inorganic salts premix (g or mg/kg feed): epsom salt, 1.8g; Ironic citrate, 0.9g; White vitriol, 0.5g; Cupric sulfate pentahydrate, 5mg; Six water alchlors, 2.5mg; KI, 2.8mg; Sodium selenite, 0.15mg; Sulfuric acid monohydrate is violent, 3.8mg; CoCL2,1.9mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g.
6. jian carp mixed feed (B), its prescription composition by weight/%:
Domestic fish meal 51.50, alanine 0.90, fish oil 1.44, corn oil 1.43, Choline Chloride 0.50, vitamin premix 0.95, inorganic salts premix 1.8, calcium dihydrogen phosphate 0.50, wheat bran 6.25, inferior powder 31.45, bentonite 2.18, alphalise starch 1.1.Described vitamin premix (mg/kg feed): thiamine hydrochloride, 65; Riboflavin, 205; Folic acid, 15; Puridoxine hydrochloride, 40; Niacin, 800; Calcium pantothenate, 280; Inositol, 400; Biotin, 5; Vit E, 415; Vit B
12, 0.11; VitA, 1.3; Vit D
3, 0.05; Vit C, 200; Described inorganic salts premix (g or mg/kg feed): epsom salt, 1.6g; Ironic citrate, 0.8g; White vitriol, 0.4g; Cupric sulfate pentahydrate, 6mg; Six water alchlors, 3mg; KI, 3mg; Sodium selenite, 0.2mg; Sulfuric acid monohydrate is violent, 4mg; CoCL2,2mg; Sodium dihydrogen phosphate, 7.5g; Potassium chloride, 2.5g.
7. promote the method for breeding of jian carp growth, enhancing immunity and anti-stress ability, it comprises:
1) artificial incubation one age the carp fingerling, the jian carp mixed feed (B) 15 days of throwing something and feeding of being satiated with food;
2) throw something and feed jian carp mixed feed (A) 1-4 after week, throw something and feed again jian carp mixed feed (B) 1-4 week;
3) set by step 2) rule continues alternately to throw something and feed jian carp mixed feed (A), (B).
8. the method for breeding of promotion jian carp according to claim 6 growth, enhancing immunity and anti-stress ability is characterized in that: after 2 weeks of jian carp mixed feed (A) of throwing something and feeding, throw something and feed 2 weeks of jian carp mixed feed (B) again.
9. the method for breeding of promotion jian carp according to claim 6 growth, enhancing immunity and anti-stress ability, it is characterized in that: jian carp mixed feed (A) is the described jian carp mixed feed of claim 2 (A).
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| CN102524573A (en) * | 2012-01-06 | 2012-07-04 | 四川农业大学 | Feed additive for jian carp |
| CN102613426A (en) * | 2012-04-19 | 2012-08-01 | 广东海洋大学 | Vitamin additive for juvenile jian carps |
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| CN103262951A (en) * | 2012-04-05 | 2013-08-28 | 连云港市金陵饲料有限公司 | Feed suitable for breeding early carp |
| CN103431202A (en) * | 2013-07-17 | 2013-12-11 | 宁波大学 | Anti-stress reinforcing agent for portunus trituberculatus and application thereof |
| CN104206907A (en) * | 2014-09-11 | 2014-12-17 | 中国科学院水生生物研究所 | Juvenile gobiocypris rarus feed and preparation method thereof |
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| CN102524573A (en) * | 2012-01-06 | 2012-07-04 | 四川农业大学 | Feed additive for jian carp |
| CN102524573B (en) * | 2012-01-06 | 2013-12-18 | 四川农业大学 | Feed additive for jian carp |
| CN103262951A (en) * | 2012-04-05 | 2013-08-28 | 连云港市金陵饲料有限公司 | Feed suitable for breeding early carp |
| CN103262951B (en) * | 2012-04-05 | 2014-07-23 | 连云港市金陵饲料有限公司 | Feed suitable for breeding early carp |
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| CN103431202A (en) * | 2013-07-17 | 2013-12-11 | 宁波大学 | Anti-stress reinforcing agent for portunus trituberculatus and application thereof |
| CN104206907A (en) * | 2014-09-11 | 2014-12-17 | 中国科学院水生生物研究所 | Juvenile gobiocypris rarus feed and preparation method thereof |
| CN104605207A (en) * | 2015-02-11 | 2015-05-13 | 通威股份有限公司 | Feed combined feeding method for carp culture |
| CN104605207B (en) * | 2015-02-11 | 2017-10-03 | 通威股份有限公司 | A kind of feed combinations feeding method of carp culture |
| CN105028978A (en) * | 2015-06-12 | 2015-11-11 | 盐城天邦饲料科技有限公司 | Feed additive capable of improving intestinal function of grass carps and application of feed additive |
| CN106359196A (en) * | 2016-09-29 | 2017-02-01 | 通威股份有限公司 | Treatment method for performing acute stress on megalobrama amblycephala |
| CN114885868A (en) * | 2022-05-30 | 2022-08-12 | 中国海洋大学 | Novel compound feed for replacing fish meal with non-daily-ration protein source for large yellow croakers and feeding method of novel compound feed |
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