WO2018199205A1 - Feed for aquatic animals - Google Patents

Abstract

The present invention addresses the problem of providing a feed which contributes to the improvement of the growth of aquatic animals. The present invention also addresses the problem of providing a feed by which the onset of disease injury can be suppressed during rearing or culturing aquatic animals. A feed comprising a nucleic acid such as RNA is prepared and fed for various purposes, for example, increasing feed intake, increasing feed conversion efficiency and improving biophylactic function.

Description

Aquatic animal feed

The present invention relates to a feed suitable for breeding or aquaculture of aquatic animals.

The world population is steadily increasing. It is estimated that there are currently 7.3 billion people, but that it will exceed 9 billion people in 2050. Given the projected population growth, there are concerns about a serious shortage of food in the near future.

７ 70% of the earth is occupied by the ocean, and food production utilizing the ocean has been reviewed to make up for food shortages. Looking at the changes in global fishery production, the catch reached a peak at around 900,000 tons after the late 1980s, whereas aquaculture production began to increase after the 1980s, It has increased rapidly, reaching about 600,000 tons in 2012 (for example, the World Fisheries and Aquaculture White Paper 2014). Thus, the importance of aquaculture is increasing in food production using the ocean.

As described above, aquaculture and fisheries are becoming popular, but the catch of fish-based feed ingredients has been decreasing, making it difficult to obtain sufficient quantities, and the aquaculture industry due to rising prices. Issues such as squeezing the management of the company have arisen.

Also, if a disease occurs in the fish and shellfish to be cultivated, a large loss will occur. For this reason, drugs such as antibiotics are sometimes used, but such drugs are often expensive and contribute to an increase in production costs. Antibiotics may lose their effectiveness as viruses and bacteria acquire drug resistance as they continue to be used. Furthermore, there is a concern about the effects on humans when fish and shellfish, which are human foods, are mixed with antibiotics and fed in large quantities. Therefore, although antibiotics are useful as a disease countermeasure, it is required to keep them to the minimum necessary.

As a countermeasure not using antibiotics or other chemicals, it has been proposed to use a fermented lactic acid bacterium obtained by culturing in a culture medium containing a mushroom waste bed as an immunostimulator for fish and shellfish (for example, patent document) 1).

Japanese Patent Laying-Open No. 2015-172019

In view of the above situation, an object of the present invention is to provide a feed that contributes to improving the growth of aquatic animals. Moreover, this invention makes it a subject to provide the feed which can suppress the disease generation | occurrence | production in breeding or aquaculture of aquatic animals.

As a result of intensive studies, the present inventors have found that the above problems can be solved by the following means, and have completed the present invention.
[1] A feed for aquatic animals containing a nucleic acid component.
[2] The aquatic animal feed according to [1] above, wherein the nucleic acid component contains ribonucleic acid and / or nucleotides.
[3] The aquatic animal feed according to claim 1 or 2, wherein the addition amount of the nucleic acid component is 0.01 to 5.0% (W / W).
[4] The feed for aquatic animals according to any one of the above [1] to [3], which is for enhancing the feeding ability of aquatic animals.
[5] The aquatic animal feed according to any one of [1] to [4] above, which is for improving feed conversion efficiency.
[6] The aquatic animal feed according to any one of the above [1] to [5], which is used for enhancing the body defense ability of aquatic animals.
[7] The aquatic animal feed according to any one of [1] to [6] above, wherein the aquatic animal is an edible seafood.
[8] A method for producing aquatic animals, comprising feeding the aquatic animal feed according to any one of [1] to [7] above.
[9] Feeding in combination with a first feed containing one or more selected from the group consisting of plant-derived feed, raw fish feed, fish meal, and fish oil, and a second feed containing a nucleic acid component The second feed is administered such that the added amount of the nucleic acid component is 0.01 to 5.0% (W / W) with respect to the first feed. , Aquatic animal production method.

ADVANTAGE OF THE INVENTION According to this invention, the feed which contributes to the growth improvement of aquatic animals can be provided.
Moreover, according to this invention, the feed which can suppress the disease generation | occurrence | production in breeding or aquaculture of aquatic animals can be provided. By using the aquatic animal feed of the present invention, the amount of antibiotic used can be suppressed.

Hereinafter, embodiments of the present invention will be described.
The aquatic animal feed of the present invention contains a nucleic acid component. In the present invention, the term “aquatic animal” means an animal that can live in water. In the present invention, the term “aquatic animal” includes seafood, amphibians and aquatic mammals, and includes both freshwater and marine animals. In the present invention, the term “fish and shellfish” includes fish, shellfish, and crustaceans.

The aquatic animal feed of the present invention is suitable as a feed for seafood. Preferred seafood to be fed with the feed for aquatic animals of the present invention include, for example, sea fish such as yellowtail, yellowtail, amberjack, hiramasa, thailand, tuna, pufferfish, flounder, horse mackerel, mackerel, grouper, cucumber, salmon, Examples include freshwater fish such as ayu, char, eel, carp, perch, crucian carp, trout, trout, smelt, goldfish, medaka, tilapia, sturgeon, and other shrimps and crabs.

Moreover, the feed for aquatic animals of the present invention can be suitably used as a feed for edible or ornamental aquatic animals.

The nucleic acid component contained in the aquatic animal feed of the present invention can be a nucleic acid or a nucleotide that is a constituent unit thereof. The type of sugar constituting the nucleic acid component may be either deoxyribose or ribose. That is, the nucleic acid component may be either deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). The types of bases constituting the nucleic acid component mainly include adenine, guanine, thymine, cytosine, and uracil. That is, the types of nucleosides that constitute the nucleic acid component include adenosine, guanosine, cytidine, uridine, and thymidine. It is done. The phosphoric acid constituting the nucleotide may be monophosphoric acid or plural phosphoric acids. Commercially available products may be used as the nucleic acid component. Nucleic acid components may be blended singly or in combination of a plurality of types.

The origin of the nucleic acid component is not particularly limited, and may be artificially synthesized or derived from a natural product. For example, those extracted or purified from microorganisms such as yeast may be used. The nucleic acid component synthesized, extracted or purified in this way can be in a form that is easily absorbed when fish eats. Biological resources made into waste materials, for example, yeast sugar and other microorganisms are grown using woody sugar contained in wood made into waste materials, nucleic acid components are obtained, and blended and used as feed of the present invention. We can convert what we had into useful substances and contribute to the formation of a sustainable recycling society.

The aquatic animal feed of the present invention may use a nucleic acid component as an active ingredient alone, or may be a mixture of a nucleic acid component and other feed components. The nucleic acid component may be (1) added to other feed components as described above and fed as a mixed feed, or (2) the nucleic acid component and the other feed components are directly used. It is also possible to adopt a form in which the nucleic acid component and other feed component are administered separately and fed together without mixing, without being mixed. In the present specification, when it is simply referred to as “combination” without particular limitation, it is used as a term including both forms (1) and (2). In addition, for convenience, the other feed (or feed component) is referred to as the first feed, and the feed or nucleic acid purified product mainly composed of the nucleic acid component used in addition to the first feed is used as the second feed. Sometimes called feed. As other feed components (first feed), feeds usually used for breeding aquatic animals can be used. Examples of the feed for seafood include plant-derived feeds such as wheat flour and soybean oil residue, live feed such as raw fish fillets, fish meal, fish oil, and mixtures thereof. In the feed for aquatic animals of the present invention, if necessary, moisture, oil, pH adjuster, antioxidant, preservative, coloring material, fragrance, excipient, vitamins, hormones, amino acids, antibiotics Etc. may be blended.

The feed for aquatic animals of the present invention may be in any form as long as it is in a form that can be ingested by aquatic animals. For example, it can be in the form of powder, granules or pellets.

The aquatic animal feed of the present invention is suitable as a feed for breeding or aquaculture of aquatic animals, but can also be suitably used for specific purposes in breeding or aquaculture. In the following description, unless otherwise specified, “breeding or aquaculture” is simply referred to as “breeding or the like”.

The feed for aquatic animals of the present invention is suitable, for example, as a feed for promoting the intake of aquatic animals. By mixing or using a nucleic acid component and other feed components such as fish meal in combination, the feeding property of aquatic animals targeted for breeding can be improved.

When used for feeding, the lower limit of the amount of nucleic acid component added is preferably 0.01% (W / W) or more, more preferably 0.03% (W / W) relative to other feeds to be fed. ) Or more, more preferably 0.05% (W / W) or more. From the viewpoint of enhancing feeding ability, the upper limit of the amount of nucleic acid component added is not particularly limited, but is preferably 5.0% (W / W) or less, more preferably 3.0% (W / W) or less. Preferably, it is 1.5% (W / W) or less. If it exceeds 5.0% (W / W), there may be a tendency that it becomes difficult to obtain an effect corresponding to the increase in the amount of addition.

Also, the aquatic animal feed of the present invention is suitable as a feed for improving feed conversion efficiency. Nucleic acid components and other feed components such as fish meal can be combined or used together to improve feed conversion efficiency in aquatic animals targeted for breeding.

When used for improving feed conversion efficiency, the lower limit of the amount of nucleic acid component added is preferably 0.01% (W / W) or more, more preferably 0.03% (W / W) relative to other feeds to be fed. W) or more, more preferably 0.05% (W / W) or more. From the standpoint of improving feed conversion efficiency, the upper limit of the amount of nucleic acid component added is not particularly limited, but for nucleotides exceeding 5.0% (W / W), the effect corresponding to the increase in the amount added Tend to be difficult to obtain.

Since the feeding property and feed conversion efficiency can be improved as described above, the weight, body length, etc. of aquatic animals can be more efficiently enhanced by the aquatic animal feed of the present invention. Moreover, the feed for aquatic animals of the present invention can contribute to growing aquatic animals sufficiently large while suppressing the total amount of feed to be fed by improving feed conversion efficiency.

The feed for aquatic animals of the present invention tends to decrease the specific liver weight when bred using this, and the given feed is not stored as an energy source in the liver and contributes to the growth of the body as it is. It is estimated that. Therefore, also from this viewpoint, the aquatic animal feed of the present invention can be suitably used for culturing edible fish and shellfish, and can perform aquaculture with good feed efficiency.

The feed for aquatic animals of the present invention is also suitable as a feed for enhancing the body defense ability of aquatic animals. The feed for aquatic animals of the present invention improves the functions related to biodefense ability such as phagocytosis, complement value, lysozyme activity of leukocytes in aquatic animals targeted for breeding by feeding, and against pathogenic bacteria, etc. Resistance can be improved.

From the viewpoint of improving the phagocytosis or complement value of leukocytes, it is preferable to use RNA as the nucleic acid component.
From the viewpoint of improving leukocyte phagocytosis or lysozyme activity, it is preferable to use nucleotides.

From the viewpoint of improving the phagocytosis of leukocytes, the lower limit of the amount of RNA added is preferably 0.1% (W / W) or more, more preferably 0.3% (W / W) or more, more preferably 0.5% (W / W) or more. On the other hand, the upper limit of the addition amount of RNA is not particularly limited, but exceeds 5.0% (W / W). And the tendency to become difficult to obtain the effect according to the increase in the added amount may appear.

From the viewpoint of improving the phagocytosis of leukocytes, the lower limit of the nucleotide addition amount is preferably 0.1% (W / W) or more, more preferably 0.3% (W On the other hand, the upper limit of the addition amount of nucleotides is preferably 5.0% (W / W) or less, more preferably 3.0% (W / W) or less.

From the viewpoint of improving complement value, it is preferable to use RNA, and the lower limit of the amount of RNA to be added is preferably 0.1% (W / W) or more, more preferably relative to other feed to be fed 0.3% (W / W) or more, more preferably 0.5% (W / W) or more. On the other hand, the upper limit of the addition amount of RNA is not particularly limited, but 5.0% ( If it exceeds (W / W), there is a possibility that a tendency to make it difficult to obtain the effect corresponding to the increase in the amount added may appear.

From the viewpoint of improving lysozyme activity, it is preferable to use nucleotides as the nucleic acid component, and the lower limit of the amount of nucleotides added is preferably 0.1% (W / W) or more, relative to other feeds to be fed. Preferably it is 0.3% (W / W) or more, more preferably 0.5% (W / W) or more. On the other hand, the upper limit of the addition amount of nucleotides is preferably 5.0% (W / W). Or less, more preferably 3.0% (W / W) or less.

As described above, from the viewpoint of enhancing the defense ability of the body, there are slight differences in efficacy depending on the type and concentration of the ingredients, but there are comprehensive applications for enhancing feeding ability, for improving feed conversion efficiency, and for enhancing the defense ability of the body. As a preferable example of the addition amount of the nucleic acid component, for example, 0.01% (W / W) to 3.0%, 0.03% (W / W) to 2.0% (W / W) or less, or 0.05% (W / W) or more and 1.5% (W / W) or less.

It is possible to produce aquatic animals by feeding the aquatic animal feed of the present invention. Production of aquatic animals may be performed in a tank containing fresh water or seawater, or in a pond, a lake, or the ocean. The number of administrations, dosage, timing of administration, etc. of the feed for aquatic animals of the present invention depend on various conditions such as the type of aquatic animals, the growth stage, the growth environment (the size of the growth place, the number and density of the aquatic animals, the water temperature, etc. Etc.) and the like can be adjusted as appropriate. The aquatic animal feed of the present invention may be used in combination with other feeds, additives, drugs such as antibiotics, or antibacterial materials such as antibacterial fine particles or nanoparticles.

By feeding the aquatic animal feed of the present invention, it is possible to breed aquatic animals while promoting the growth efficiency of aquatic animals and suppressing infection with pathogenic bacteria. Moreover, since the biodefense ability of aquatic animals can be improved by using the feed for aquatic animals of the present invention, the use of antibiotics can be stopped or used. In addition, the fish and shellfish produced using the aquatic animal feed of the present invention has no particular problem in taste, and the aquatic animal feed of the present invention can be suitably used as a feed for culturing edible fish and shellfish.

Hereinafter, the present invention will be specifically described with reference to examples, but the technical scope of the present invention is not limited to the following examples.

<Examples 1 to 6>
RNA or nucleotide was added to the base feed so as to have a predetermined addition amount, and the feeds of Examples 1 to 6 and the control feed were prepared.

As RNA added to the base feed, RNA-M (manufactured by Nippon Paper Industries Co., Ltd.), which is a nucleic acid derived from yeast, was used.
As nucleotides added to the base feed, NPC nucleotides (manufactured by Nippon Paper Industries Co., Ltd.), which are nucleic acids derived from yeast, were used.
As a base feed, a feed having a composition according to a commercial feed (Nisshin Marubeni Feed Co., Ltd.) was used.

The amount of nucleotide and RNA added to the base feed component in each feed is as shown in Table 1. Moreover, the composition of the base feed is as shown in Table 2. Each feed used was formed into pellets having a diameter of about 2 mm and / or 3 mm depending on the size of the test fish.

<Growth test 1>
(1) Test fish and breeding method The rainbow trout fry was raised using Examples 1 to 6 and the control feed as feed, and the growth was tested. The base feed shown in Table 2 was used as the control feed.

40 rainbow trouts were housed in a 100 L FRP (Fiberglass Reinforced Plastic) water tank to form one test zone. In the aquariums of each test section, satiation feeding was performed twice a day while performing aeration by flowing water type (24 rotations a day), and reared for 90 days under the condition of an average water temperature of 20 ° C. ± 3 ° C. As a standard for satiety feeding, the feeding ability of rainbow trout was clearly reduced, and a small amount of residual food settled on the bottom of the aquarium was regarded as satiety feeding.

(2) Measurement of feeding amount, body weight and length, and calculation of feed conversion efficiency The weight of feed was measured before and after all feedings, and the daily feeding amount was calculated and recorded daily. The average feeding amount was used as an index of food intake.

Every 30 days, the body weight and length of all rainbow trouts in each test group were measured under anesthesia with 2-phenoxymethanol (manufactured by Wako Pure Chemical Industries, Ltd.), and the average value for each test group was determined.
Moreover, the feed conversion efficiency (increased body weight / feeding amount) × 100 for each test section throughout the entire test period was determined from the above feeding amount and fish weight.

(3) Liver weight and specific liver weight value The control test group and the test group of Example 5 after 18 days from the end of the above 90-day rearing, the test group of Examples 2, 3, 4 and 6 after 20 days, and 21 days Later, for all the test fish in the test section of Example 1, the fish body weight and standard body length were measured under ice-cold anesthesia, and then the rainbow trout whole blood was collected with a heparinized syringe and then dissected. All organs except for were removed. All the extracted organs and the weight of the liver were measured. Liver weight relative to body weight per individual was calculated as specific liver weight value (%).

(4) Test results Tables 3 to 8 show the results of feeding amount, fish body weight, fish body length, feed conversion efficiency, liver weight, and specific liver weight values obtained as described above.

The average feed amount, fish body weight, and fish body length tended to be approximately the same or significantly higher in all test groups of Examples 1 to 6 than in the control test group.
Regarding the feed conversion efficiency, all the test groups of Examples 1 to 6 in the Examples showed higher values than the control test group.

Regarding the liver weight, in each of the test groups of Examples 1, 3, and 4, there was a tendency to significantly increase with respect to the control test group, and also in the test groups of Examples 2 and 5, compared to the control test group. Equivalent or higher value. However, only the test group of Example 6 resulted in a significant decrease compared to the control test group, suggesting that there is an upper limit on the preferred dose from the viewpoint of increasing the liver weight.

Regarding the specific liver weight value, in any of the test groups of Examples 1 to 6, the test group of Example 2 showed a significantly lower value than the control test group (1.05 ± 0.40%). A low 0.8 ± 0.1 (more specifically 0.79 ± 0.11%) was shown. The fish liver is generally known to play a role as an energy storage organ (Hideo Akiyoshi et al., 2001, “Comparative Morphological Study on the Behavior of Marine Fish and Histo-Biochemical Correlation”, Shimane University Bioresources Research As shown in the report No. 6) and the results of the growth test described above, the feed rate of Examples 1 to 6 was also improved because the growth rate when the feed containing nucleic acid was satiety fed daily was improved. It was speculated that it was used for growth without storing energy.

<Bioprotection test>
(1) Test fish and rearing method Tests were conducted on biological defense ability using rainbow trout using Examples 2, 3, 5, 6 and control feed as feed.

The test fish was prepared as follows. Rainbow trout larvae were bred in a 1.1 t FRP aquarium for about 2 months until the start of this test, three times a day in the morning, noon, and evening satiation. Thereafter, for 3 months until the start of the test, a satiety feeding was performed once a day in a 1.1 t FRP water tank. The standard for satiety feeding is the same as in the growth test. Under the anesthesia with 2-phenoxyethanol (manufactured by Wako Pure Chemical Industries, Ltd.) at the start of the test, the fish weight and standard length of the test fish were measured, and 35 fish per tank were divided into 6 500L FRP water tanks. The test section was set up.

One type of each feed of the feeds of Examples 2, 3, 5, and 6 was assigned to each test group. Two test groups of control feed were provided, which were designated as control test groups 1 and 2, respectively.
In each test group, 2% of the fish weight was fed once a day and reared for 5 weeks. However, food was stopped the day before sampling.

(2) Sampling Sampling was carried out for 7 animals in each test group 1, 3, and 5 weeks after the start of feeding. The sampled test fish was measured for fish weight and standard body length under anesthesia with 2-phenoxyethanol (manufactured by Wako Pure Chemical Industries, Ltd.), and after collecting whole blood from the caudal part using a heparinized syringe Aseptically dissected and the head kidney was removed. The collected blood was dispensed in a 1.5 mL small centrifuge tube, taking care not to hemolyze, and then centrifuged at 831 × g for 15 minutes at 4 ° C., and the resulting plasma was collected at −80 ° C. Stored frozen until use.

(3) Measurement of phagocytic activity of head kidney-derived leukocytes After collecting whole blood from the test fish, the head kidney was aseptically removed immediately and tweezers were placed in a 1.5 mL small centrifuge tube containing 500 μL of RPMI-1640 medium. Was used to extrude and disperse white blood cells. After standing for 10 minutes, 100 μL of a layer containing a large amount of white blood cells was collected, added to a small centrifuge tube containing 100 μL of opsonized zymosan, and cultured at 23 ° C. for 1 hour. Thereafter, the mixture was centrifuged at 277 × g for 10 minutes at 4 ° C., the supernatant was removed, and the suspension was suspended in 100 μL of inactivated fetal bovine serum. This suspension was dropped onto a slide glass and dried, and then stained with Maygrunwald solution (manufactured by SIGMA) for 5 minutes and 50% diluted Maygrunwald solution for 10 minutes. Further, after washing twice with Giemsa staining solution [Gimsa (manufactured by MERCK): distilled water = 1: 20], staining was performed with the same solution for 15 minutes. After dyeing, washing with tap water and drying were followed by microscopic observation (x1,000). In each test group, only 300 neutrophils and macrophages capable of phagocytosis were counted, and the ratio of the number of cells phagocytosing zymozan was taken as the phagocytosis rate. The test results of the phagocytosis rate are shown in Table 9.

(4) Measurement of complement value 25 μL each of plasma collected from the test fish and red blood cells of sheep (Japan Lamb) were added and reacted at room temperature for 1 hour. After completion of the reaction, 50 μL each of EGTA · Mg ²⁺ · GGVB (a buffer solution in which 10 mM glycol ether diamine tetraacetic acid and 40 mM MgCl ₂ were added) was added at 3,000 × g for 3 minutes at 4 ° C. And centrifuged. The supernatant was transferred to a 96-well plate, and the absorbance at a wavelength of 492 nm was measured with a microplate reader. From the measured absorbance, the hemolysis rate y is determined as {y = [absorbance of specimen−absorbance of CB (mechanical hemolysis, red blood cell 25 μL + buffer 75 μL)] / [absorbance of 100% hemolysis−absorbance of CB]}. It was set as the value. The test results of complement value are shown in Table 10.

(5) Measurement of lysozyme activity The suspension of Micrococcus lysodeikticus (manufactured by SIGMA) was adjusted so that the absorbance at a wavelength of 492 nm was 0.8 using PBS 7.0 (manufactured by Nissui) at pH 7.0. did. After 120 μL of this suspension was added to each well of a 96-well plate (manufactured by IWAKI), 10 μL of plasma was added and reacted for 60 minutes. The lysozyme activity was determined as a decrease in absorbance by measuring absorbance at a wavelength of 492 nm immediately after mixing and after reaction with a microplate reader. The test results of lysozyme activity are shown in Table 11.

The results shown in Tables 9 to 11 indicate that the effects of phagocytosis, complement value, and lysozyme activity, which are indicators of innate immunity, differ between RNA and nucleotide, and the effects may vary depending on the concentration of the compound It was.

<Taste test and nutrition analysis>
(1) Test fish and breeding method For the test fish, rainbow trout, a year-old fish purchased from a seedling producer was used. The test fish was pre-bred for about two months in a 1.1t FRP tank in the freshwater breeding ridge, and then 40 rainbow trouts were accommodated in each of the seven 100L tanks. And was reared. Thereafter, it was confirmed that there were no abnormal fish or dead fish 2 weeks before the start of the test, and this test was started. The amount of water supply in each water tank was adjusted to rotate 24 times in calculation per day. The water temperature during the test period was 17.8 to 19.8 ° C. Feeding was carried out by feeding the following test feed twice a day at 9:00 and 15:00, and after 16:00, the remaining feed and feces were removed.

(2) Taste test Using 10 non-smoker Kochi University students (male: female = 4: 6) as a panel, the cooked rainbow trout was eaten and the taste was evaluated. Appearance, aroma, taste, and texture were evaluated as items of taste.

As test fish, those in the test group using the feed of Examples 1, 2, 4, and 5 and those in the control test group were used. In order to provide food, we don't give preconceptions to the panel. As for the order of provision, we decided to order Meunier, Nanbanzuke and Kanro-ni, taking into consideration that the deep seasoning of the previous dish does not affect the next dish.

According to the evaluation of the panel, there is no evaluation that the taste of the rainbow trout dish obtained from the test section of the example is inferior to that of the test section, especially the taste of fish bred using the feed of the present invention. It was confirmed that there was no problem.

(3) Nutritional analysis The content of nutritional components in rainbow trout fish meat was analyzed by commissioning the Japan Food Functional Analysis Laboratory and the Japan Food Analysis Center to compare Examples 2 and 4 and the control test area. . As a specimen, 800 g of fish meat was used for each test section. The analysis results are shown in Table 12.

As shown in Table 12, in the test group of the example and the control test group, all of the nutrient components were almost the same value, but the test group of the example had a lipid content as compared with the control test group. And docosahexaenoic acid (DHA) content tended to be high.

<Examples 7 and 8>
A feed of Example 7 was prepared in the same manner as the feed of Example 1 except that the amount of RNA added was 0.1% by weight. Further, a feed of Example 8 was prepared in the same manner as the feed of Example 4 except that the amount of nucleotide added was 0.1% by weight.

<Growth test 2>
The test was conducted using yellowtail, a saltwater fish, instead of rainbow trout, a freshwater fish.

(1) Test fish and rearing method For each test section using the feed of Example 1, Example 2, Example 4, Example 5 and the control feed, and the feed of Examples 7 and 8 as feed Of fry.

60 litters were housed in a 100 L FRP water tank, and one test zone was prepared for each feed. In the aquarium of each test area, a satiety feeding was performed twice a day while performing aeration by flowing water (24 rotations a day), and the mice were raised for 9 weeks under the condition of 23 ° C. ± 3 ° C. As a standard for satiety feeding, the feeding ability of the yellowtail was clearly reduced, and a state where a small amount of residual food settled on the bottom of the aquarium was regarded as satiety feeding.

(2) Liver weight After 6 weeks and 9 weeks of breeding, 30 fish were sampled in each test area, and after measuring the body weight and standard length of the fish under ice-cooled anesthesia, The whole blood was collected and then dissected, and all organs except the kidney were removed. All the extracted organs and the weight of the liver were measured. Table 13 shows the measurement results.

Regarding the liver weight, in each of the test groups of Examples 1, 2, 4 and 7, there was a tendency to significantly increase with respect to the control test group, and also in the test groups of Examples 5 and 8, compared with the control test group. Almost the same value.

Claims (9)

1. Aquatic animal feed containing nucleic acid components.
2. The feed for aquatic animals according to claim 1, wherein the nucleic acid component comprises ribonucleic acid and / or nucleotides.
3. The feed for aquatic animals according to claim 1 or 2, wherein the addition amount of the nucleic acid component is 0.01 to 5.0% (W / W).
4. The aquatic animal feed according to any one of claims 1 to 3, which is used for enhancing the feeding ability of aquatic animals.
5. The aquatic animal feed according to any one of claims 1 to 4, which is used for improving feed conversion efficiency.
6. The aquatic animal feed according to any one of claims 1 to 5, which is used for enhancing the body defense ability of aquatic animals.
7. The aquatic animal feed according to any one of claims 1 to 6, wherein the aquatic animal is an edible seafood.
8. A method for producing aquatic animals, wherein the aquatic animal feed according to any one of claims 1 to 7 is fed.
9. Aquatic animals that are fed together with a first feed containing one or more selected from the group consisting of plant-derived feed, raw fish feed, fish meal, and fish oil, and a second feed containing nucleic acid components The second feed is an aquatic animal administered such that the amount of the nucleic acid component added is 0.01 to 5.0% (W / W) relative to the first feed. Production method.