CN103549448A - Preparation method of amino acid nutrient solution - Google Patents

Preparation method of amino acid nutrient solution Download PDF

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Publication number
CN103549448A
CN103549448A CN201310518763.0A CN201310518763A CN103549448A CN 103549448 A CN103549448 A CN 103549448A CN 201310518763 A CN201310518763 A CN 201310518763A CN 103549448 A CN103549448 A CN 103549448A
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China
Prior art keywords
amino acid
preparation
amino
protein complex
nutrient solution
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CN201310518763.0A
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Chinese (zh)
Inventor
黄俊逸
葛家福
孙荣鑫
虞翔
陈珍
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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Priority to CN201310518763.0A priority Critical patent/CN103549448A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/02Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/30Meat extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention relates to a preparation method of an amino acid nutrient solution. The efficient amino acid nutrient solution prepared by the preparation method is derived from the meat of a newborn bull and contains 18 amino acids (include 8 amino acids essential for a human body) needed for human growth, development, tissue repair and anti-aging, 15 trace elements, B vitamins and other nutrients. The efficient amino acid nutrient solution is in a liquid form and uniform in color and luster, has a color of brown orange, tastes slightly sweet and sour, is free of foreign objectionable odor, contains more than 2% of protein and 2.5% of total sugar, cannot be influenced by gastrointestinal function, and is easily directly absorbed and utilized by the human body.

Description

The preparation method of amino-acid nutrition liquid
Technical field
The present invention relates to a kind of preparation method of amino-acid nutrition liquid.
Background technology
China is milk cattle cultivating big country, has abundant little bull calf resource, and approximately 1,400 ten thousand of national existing milk cow quantity, can produce approximately 3,000,000 of little bull calfs every year.The resource huge to this, applies the progressively in addition comprehensive exploitation application of modern mature science and technology, has very profound significance.If bull calf is raised as beef cattle, because its production cycle is long, herbage and feed is in short supply, breeding technology lags, production cost is high, therefore the economic benefit producing is just very poor.Therefore after calf birth, major part is eliminated, and sells to and butchers processing enterprise, makes this valuable resource fail to obtain sufficient Application and Development.This team finds in research and development of products, and little bull calf whole body is all precious, as the meat of little bull calf contains rich in protein and active material, through modern biotechnology and abstraction technique, can extract the elite in beef, makes effective amino-acid nutrient solution ,can meet the demand of more crowd to nutrition and health care.
Root according to data, due to features such as amino acid whose trophism, nourishing, health, security, high-absorbables, have different physiological roles, therefore containing the product of amino acids, obtained abroad attention and the research and development of height, the exploitation of You Yimei, two countries and product sold are maximum.Its major product has amino acid nutrient transfusion, amino acid sports drink, high-energy beverage, cold drink etc.While having for human body emerency request as the U.S. or when abilities of digestive and absorption reduces " the amino acid nutrient replenishers " of essential nutritional supplementation.Have and take essential amino acid as main amino acid mixing transfusion; Have and be applied to hypotensive preparation and anti-virus formulation etc., the commodity that also have the various formulations such as tablet, capsule are commercially available.
Amino acid energy beverage and sports drink's " amino acid biogen " of Japan aginomoto company Development and Production, contain 12 seed amino acids centered by leucine, isoleucine, 3 kinds of branched chain amino acids of valine and glutamic acid, arginine in product.These 3 kinds of branched chain amino acids have the muscle of formation, strengthening liver function, alleviate the effects such as muscular fatigue, and sportsman's muscle protein in vigorous exercise can be decomposed, and drinks the decomposition that can suppress protein in muscle before motion.This product improves endurance to sportsman and produces effect very much.Japan Suntory Ltd., kylin company, Mingzhi drugmaker, health hall company etc. all develop containing products such as all kinds of amino acid whose beverages, high to this type of product evaluation in the world.The U.S. " also there are a lot of these series products in Coca-Cola ”Deng company, " A Geli is happy " as by name in product sports drink, and 250ml-2L, containing L arginine 0.02%, in gymnasium, sports ground consumption is very large.The U.S. also researches and develops the new product " amino acid life beverage " of releasing after improving, containing amino acid 2200mg, the effect of there is explosive force, reacting at a high speed, this product is being allocated on leucine, valine, isoleucine, glutamic acid, arginic basis, add 6 kinds of glucogenic amino acids of rapid switching energy in vivo, they are alanine, serine, proline, cysteine, aspartic acid, threonine again.
The advanced technologies such as that Japan adopts is concentrated, enzyme reaction and biochemistry, Biological Technology, biotechnology, the refining novel foodstuff forming has reached more than 4000 s of 2 Wan Duozhong, manufacturing enterprises, and annual sales amount reaches tens billion of dollars.China's application modern science and technology, production has health care and also has development fast with the food cause of trophic function, and manufacturing enterprise has surpassed thousands of families, nearly ten thousand kinds of the kinds of production.Enter for many years the amino acid product in market, Shanghai, most employing vegetable proteins or at a low price animal protein are done raw material hydrolysis and are formed, and their amino acid contained kinds are incomplete, mutual ratio is also undesirable, having plenty of cooperation and form, also do not list constant and micronutrient levels, is a defect.Product identical with " effective amino-acid nutrient solution " content on market is not also found.
After the food that our feed contains protein, all must be through the hydrolysis of pipe intestinal digesting enzyme, just can be absorbed after dividing ten million " amino acid ", and then be synthesized the protein of needed by human body.Wherein having eight seed amino acids is that human body can not synthesize, must be obtained by food supply, be called as " essential amino acid ", when liver utilizes amino acid amalgamation to manufacture protein, all need complete " amino acid ", comprise can manufacture and eight kinds of external " essential amino acids " own, for want of if a certain " amino acid ", just can not manufacture this kind of protein, and cause the generation of disease, therefore amino acid is the compound that a class has special significance, it is the essential base substance of vital movement, protein synthetic muscle in vivo, skin, various internal organs, enzyme, the necessary constituent such as immune antiboidy, it is the requisite material of human body.
Modern nutriology man studies discovery, the mankind ingest protein after the hydrolysis of alimentary canal enzymatic, separation and purification obtains " amino acid ", " polypeptide " mixture and (2-3 amino acid or 3, with upper amino acid, consists of, less than protein molecule quality, simple in structure, the material that physiologically active is strong is called as small-molecular peptides and polypeptide).Scientist studies discovery, the amino acid of little peptide specific ionization is more easily absorbed by body, some little peptide can not only provide people's bulk-growth, grow needed nutriment, but also there is unique biological function, can prevent and treat thrombus, high fat of blood, hypertension, delay senility, resisting fatigue, raising immunity of organisms etc., be the outer the most popular research topic of food nutrition expert of Present Domestic.Therefore, amino-acid nutrition liquid and biological polypeptide technology will become the focus of 21 century biotechnology, become modern high technology industry to the ground zero of darker field development.
Since nearly over half a century, life science has obtained development extremely rapidly, makes people further recognize dietetic nutrition with healthy relation and how by supplementing with science diet of nutrient, to remove to regulate body function, carries out the prevention of disease.And effective amino-acid nutrient solution contains abundant nutrient, so this product of active development has very important meaning to improving each age group crowd's health, surely can be welcomed by the people, and market prospects are fine, have a high potential.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of amino-acid nutrition liquid.
For achieving the above object, the present invention adopts following technical scheme:
A preparation method for amino-acid nutrition liquid, is characterized in that the concrete steps of the method are:
a. the raw material of nutrient solution and quality percentage composition thereof are:
Nascent bull calf beef 85%--90%
Acidic protein complex enzyme 0.5%--1.2%
Basic protein complex enzyme 0.5%--1.2%
Caramel 3.0%--4.0%
Honey 3.0%--5.0%
Pure water surplus
B. the bull calf beef of coming into being adds pure water to make uniform slurry after rubbing, regulating pH is 4.5~6.5, at 40~50 ℃ of temperature, adds acidic protein complex enzyme to carry out enzymolysis, then to regulate pH be 7.5~8, at 40~50 ℃ of temperature, add basic protein complex enzyme to carry out enzymolysis; Add again all the other components to allocate, after filtration and ultrafiltration, concentrated, sterilizing, obtain amino-acid nutrition liquid.
Above-mentioned acidic protein complex enzyme is: bromelain and papain.
Above-mentioned basic protein complex enzyme is: trypsase and papain.
The prepared effective amino-acid nutrient solution of the application is containing nutrients such as people's bulk-growth, growth, tissue repair, the 18 required seed amino acids that delay senility (8 seed amino acids that comprise needed by human), 15 kinds of trace elements and B family vitamins; Product be color and luster evenly, the liquid state of brown color, taste micro-sour-sweet, without external bad smell, protein content higher than 2%, total sugar content is higher than 2.5%.This product is not affected by gastrointestinal function, easily directly by human body, is absorbed and utilizes.
The specific embodiment
Embodiment mono-:
1] process of production:
Take 10 kilograms, safe little bull calf beef of originating, be twisted into meat gruel, put into the appropriate water of 45 ℃, stir; With acetic acid, regulate its pH value to 5, then add the acidic protein complex enzyme of 0.1 kilogram, after stirring, be hydrolyzed 6 hours; With sodium bicarbonate, regulate its pH value to reach 8 subsequently, add the basic protein complex enzyme of 0.1 kilogram, after stirring, be hydrolyzed 6 hours.
Its hydrolyzate, 10,000 * g high speed centrifugation 20 minutes, is removed residue.Supernatant regulates near its pH value to 6 with acetic acid again, adds 3 kilograms of caramel and honey kilogram, with ultra-pure water constant volume to 100 liter.After filtration and ultrafiltration, its filtrate was 121 ℃ of sterilizings 20 minutes, and packing, obtains required amino-acid nutrition liquid.
We invent effective amino-acid nutrient solutionthe mean value of the various content 1-3 that sees the following form.
Figure 827343DEST_PATH_IMAGE001
Production overall process, carries out according to rules such as People's Republic of China's health legislation and quality safeties completely.Whole production procedure, theory and the technology of having applied the subjects such as modern biotechnology, Food Science, nutrition, physiology collaborate utilization, strictly control material quality, science enzymolysis, adopt modern abstraction technique, extraction nutrition elite, carries out the operation standards such as science preparation, concentrated, sterilizing, filling, quality inspection and produces by product requirement.
2] product sensory index;
Product color: color and luster uniformity, is brown color
Flavour and smell: taste is sweet, without external bad smell
Structural state: be uniform liquid, foreign, deposit-free
3] product physical and chemical index;
Protein %≤2
Total reducing sugar (in reduced sugar) %≤2.5
pH 5—6.5
4] packaging material and specification
Effective amino-acid nutrient solution packaging material: vial
Packing specification: 10/box of every/15ml
5] main feature of product;
1. domestic breast bull calf aboundresources, cheap;
2. pollution-free, the pure natural of raw material;
3. product is without hormone, non-pigment, drug residue free, containing anticorrisive agent;
4. product is containing high nutrient, low calorie, no antigen, easily absorption;
5. production process is without waste gas, waste sludge discharge, and noiselessness affects;
6. product energy adjustment human body metabolism, strengthens immunity of organisms, promotes child and teenagers healthy growth, improves athletic locomitivity, to oldaged physically weak person, can build up health, and improves the quality of living, delays senility, promotes longevity;
7. produce market prospect is good, reduced investment, instant effect.
The present invention, by the needs to the different content of amino acid and trace element according to different crowd, produces series of products.

Claims (3)

1. a preparation method for amino-acid nutrition liquid, is characterized in that the concrete steps of the method are:
A. the raw material of nutrient solution and quality percentage composition thereof are:
Nascent bull calf beef 85%--90%
Acidic protein complex enzyme 0.5%--1.2%
Basic protein complex enzyme 0.5%--1.2%
Caramel 3.0%--4.0%
Honey 3.0%--5.0%
Pure water surplus
B. the bull calf beef of coming into being adds pure water to make uniform slurry after rubbing, regulating pH is 4.5~6.5, at 40~50 ℃ of temperature, adds acidic protein complex enzyme to carry out enzymolysis, then to regulate pH be 7.5~8, at 40~50 ℃ of temperature, add basic protein complex enzyme to carry out enzymolysis; Add again all the other components to allocate, after filtration and ultrafiltration, concentrated, sterilizing, obtain amino-acid nutrition liquid.
2. the preparation method of amino-acid nutrition liquid according to claim 1, is characterized in that described acidic protein complex enzyme is: bromelain and papain.
3. the preparation method of amino-acid nutrition liquid according to claim 1, is characterized in that described basic protein complex enzyme is: trypsase and papain.
CN201310518763.0A 2013-10-29 2013-10-29 Preparation method of amino acid nutrient solution Pending CN103549448A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724683A (en) * 2005-07-15 2006-01-25 中国海洋大学 Method of preparing peptide having trypsin inhibition activity by using complex enzyme enzymolysis oyster
CN101982114A (en) * 2010-09-26 2011-03-02 广东海洋大学 Method for producing anti-fatigue oral liquids by using Pinctada martensii dunker meats
CN101982113A (en) * 2010-09-26 2011-03-02 广东海洋大学 Preparation method of crassostrea gigas meat antifatigue nutrient solution
CN102415546A (en) * 2011-12-09 2012-04-18 广州市味研生物工程科技有限公司 Production process for seafood extract

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724683A (en) * 2005-07-15 2006-01-25 中国海洋大学 Method of preparing peptide having trypsin inhibition activity by using complex enzyme enzymolysis oyster
CN101982114A (en) * 2010-09-26 2011-03-02 广东海洋大学 Method for producing anti-fatigue oral liquids by using Pinctada martensii dunker meats
CN101982113A (en) * 2010-09-26 2011-03-02 广东海洋大学 Preparation method of crassostrea gigas meat antifatigue nutrient solution
CN102415546A (en) * 2011-12-09 2012-04-18 广州市味研生物工程科技有限公司 Production process for seafood extract

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Application publication date: 20140205