CN103549252A - Preparation method and application of sugarcane polysaccharide extract - Google Patents
Preparation method and application of sugarcane polysaccharide extract Download PDFInfo
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- CN103549252A CN103549252A CN201310498983.1A CN201310498983A CN103549252A CN 103549252 A CN103549252 A CN 103549252A CN 201310498983 A CN201310498983 A CN 201310498983A CN 103549252 A CN103549252 A CN 103549252A
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- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 268
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 268
- 150000004676 glycans Chemical class 0.000 title claims abstract description 199
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 199
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 199
- 238000002360 preparation method Methods 0.000 title claims abstract description 82
- 239000000284 extract Substances 0.000 title claims abstract description 49
- 238000000605 extraction Methods 0.000 claims abstract description 85
- 235000013361 beverage Nutrition 0.000 claims abstract description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims description 65
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 238000000034 method Methods 0.000 description 50
- 239000012141 concentrate Substances 0.000 description 30
- 239000000047 product Substances 0.000 description 23
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 17
- 229930006000 Sucrose Natural products 0.000 description 17
- 239000005720 sucrose Substances 0.000 description 17
- 238000011084 recovery Methods 0.000 description 15
- 238000001514 detection method Methods 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 241000068415 Cynanchum bungei Species 0.000 description 10
- 241000590988 Danainae Species 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000011156 evaluation Methods 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 9
- 239000002994 raw material Substances 0.000 description 9
- 238000001035 drying Methods 0.000 description 7
- 239000000706 filtrate Substances 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 6
- 235000019634 flavors Nutrition 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000000205 computational method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000009413 insulation Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000012856 packing Methods 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 238000012797 qualification Methods 0.000 description 5
- 230000003068 static effect Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000000259 anti-tumor effect Effects 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000003292 diminished effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002218 hypoglycaemic effect Effects 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 239000012445 acidic reagent Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 150000002772 monosaccharides Chemical group 0.000 description 2
- 238000004506 ultrasonic cleaning Methods 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000798443 Hornodermoporus martius Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of plant extraction and discloses a preparation method and application of a sugarcane polysaccharide extract. The preparation method of the sugarcane polysaccharide extract provided by the invention comprises the following steps of: collecting sugarcane leaves and/or sugarcane tips to obtain a first product; mixing the first product with water in the mass ratio of 1: (10-50), extracting for 60-150 min at a temperature ranging from 70 to 100 DEG C, and collecting the extract to obtain the sugarcane polysaccharide extract. The preparation method of the sugarcane polysaccharide extract provided by the invention is few in steps and simple in preparation process; the sugarcane leaf and sugarcane tip resources are utilized sufficiently, and the obtained sugarcane polysaccharide extract contains rich sugarcane polysaccharides and can be applied to preparing sugarcane polysaccharide-containing beverages.
Description
Technical field
The invention belongs to technical field of plant extraction, particularly the preparation method and application of sugarcane polysaccharide extraction liquid.
Background technology
Sugarcane is a kind of annual or perennial root Perenniporia martius herbaceous plant, is the important source material of manufacturing sucrose, and cultivated area is wide and have a higher economic worth.Sugarcane stem is upright, sturdy succulence, and white powder is often draped over one's shoulders on surface, and leaf is alternate, the little sharp mouth shape of edge tool, Hua Sui is compound raceme.Sugarcane taste is sweet, puckery, and property is flat, nontoxic, wherein contains the materials such as abundant sugar, moisture, multivitamin, protein, calcium, iron, has the effect of clearing heat and promoting fluid, is quite subject to liking of people.In China, sugarcane essentialspecies is implanted in the ground such as Guangxi and Yunnan, and annual production reaches more than one hundred million tons, in a large amount of sugarcane of results, has also produced many sugarcane tops and sugarcane toppers every year.Sugarcane top is one of important feature of sugarcane, by carrying out the growth that photosynthesis is sugarcane, provides energy and material.Sugarcane toppers, also claims sugarcane tail, cuts down the general designation of 2～3 the tender joints in top and dark green blade while being results sugarcane.Sugarcane top and/or sugarcane toppers are the byproducts of sugarcane, wherein contain abundant sugar, protein, vitamin etc.Yet, at present to the application of sugarcane top and/or sugarcane toppers except part is for animal feeding-stuff, all the other major parts often go out of use, burn, and waste resource contaminated environment again.
Plant polyose, is called again plant polysaccharide, is that plant metabolism produces, and it is formed by connecting by glycosidic bond by more than 10 or 10 monosaccharide groups conventionally, has straight sugar chain or a sugar chain structure.There is bioactive plant polyose, often by 100 above even several thousand monosaccharide groups form, there is clear and definite three-dimensional structure, and together with polypeptide, albumen or contaminated with lipid.Research is found all to contain abundant plant polyose in sugarcane top and sugarcane toppers, belongs to sugarcane polysaccharide, and it has the immunity of organisms of enhancing, the effect such as antibiotic, antitumor and hypoglycemic.
At present, need to be through immersion the steps such as extraction, alcohol precipitation, separation about the extraction of sugarcane polysaccharide in sugarcane top and/or sugarcane toppers, its complicated operation, complex steps, is not suitable for suitability for industrialized production.So at present,, about the report of sugarcane top and/or the rational industrial applications of sugarcane toppers, the sugarcane polysaccharide resource in sugarcane top and/or sugarcane toppers is not fully utilized.
Summary of the invention
In view of this, the invention provides a kind of preparation method and application of sugarcane polysaccharide extraction liquid.Preparation method's step of this sugarcane polysaccharide extraction liquid is few, preparation technology is simple, takes full advantage of sugarcane top and sugarcane toppers resource, and gained sugarcane polysaccharide extraction liquid contains abundant sugarcane polysaccharide, can be applied to preparation containing the beverage of sugarcane polysaccharide.
In order to realize object of the present invention, the present invention adopts following technical scheme:
The preparation method who the invention provides a kind of sugarcane polysaccharide extraction liquid, comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
Indication sugarcane toppers of the present invention, also claims sugarcane tail, cuts down the general designation of 2～3 the tender joints in top and dark green blade while being results sugarcane.
By percentage to the quality, in the sugarcane polysaccharide extraction liquid being prepared into according to this preparation method, the content of sugarcane polysaccharide is 0.022%～0.118%.
Preparation method provided by the present invention, preparation technology is simple, and the sugarcane polysaccharide extraction liquid being prepared into contains abundant sugarcane polysaccharide.Because having, sugarcane polysaccharide strengthens immunity of organisms, the effect such as antibiotic, antitumor and hypoglycemic, so the sugarcane polysaccharide extraction liquid that preparation method provided by the invention is prepared into also has, strengthen immunity of organisms, the effect such as antibiotic, antitumor and hypoglycemic, can be applied to preparation and there is immunity of organisms, antibiotic, antitumor and hypoglycemic medicine or the beverage of enhancing.Meanwhile, in the sugarcane polysaccharide extraction liquid that this preparation method is prepared into, also contain the materials such as some protein, vitamin, these materials make sugarcane polysaccharide extraction liquid when abundant sugarcane polysaccharide is provided, have also produced unique local flavor.
Preferably, in preparation method provided by the invention, the mass ratio of the first product and water is 1:15～25.
More preferably, in preparation method provided by the invention, the mass ratio of the first product and water is 1:20.
In some embodiments of the invention, when in preparation method provided by the invention, the mass ratio of the first product and water is 1:20, by percentage to the quality, in the sugarcane polysaccharide extraction liquid being prepared into according to this preparation method, the content of sugarcane polysaccharide is 0.047%.
Preferably, the temperature of extracting in preparation method provided by the invention is 85 ℃.
The time of preferably, extracting in preparation method provided by the invention is 90min～120min.
The time of more preferably, extracting in preparation method provided by the invention is 120min.
In some embodiments of the invention, preparation method provided by the invention also comprises concentrated step after collecting extract.
In some embodiments of the invention, in preparation method provided by the invention, before collecting sugarcane top and/or sugarcane toppers, also comprise:
Sugarcane top and/or sugarcane toppers are cleaned, residual for fear of chemical reagent in sugarcane polysaccharide extraction liquid, cleaning method of the present invention is preferably Ultrasonic Cleaning.The sugarcane top that collection cleans up and/or sugarcane toppers, obtain the first product.
The sugarcane top adopting due to preparation method provided by the invention and/or the cleaning of sugarcane toppers are preferably Ultrasonic Cleaning, and in leaching process, solvent for use is water, whole process is not added any chemical reagent, has fully guaranteed residual without chemical reagent of gained sugarcane polysaccharide extraction liquid.
The present invention also provides a kind of sugarcane polysaccharide extraction liquid to contain the application in the beverage of sugarcane polysaccharide in preparation, and the preparation method of this sugarcane polysaccharide extraction liquid comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
The present invention also provides a kind of beverage, and it comprises acceptable food additives on sugarcane polysaccharide extraction liquid and beverage, and the preparation method of this sugarcane polysaccharide extraction liquid comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
Preferably, by percentage to the quality, in beverage provided by the invention, the content of sugarcane polysaccharide is 0.044%～0.236%.
In some embodiments of the invention, the raw material of this beverage is sugarcane polysaccharide extraction liquid provided by the invention, water and sucrose.In the preparation process of beverage, can first to sugarcane polysaccharide extraction liquid provided by the invention, concentrate, obtain sugarcane polysaccharide and extract concentrate, by percentage to the quality, the content that gained sugarcane polysaccharide extracts sugarcane polysaccharide in concentrate is 0.22%～1.18%; In this preparation method of beverage, beverage provided by the invention is comprised of sugarcane polysaccharide extraction concentrate, the water of 53～75 weight portions and the sucrose of 5～10 weight portions of 15～25 weight portions;
The preparation method of this sugarcane polysaccharide extraction liquid comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
In some embodiments of the invention, the raw material of this beverage is sugarcane polysaccharide extraction liquid provided by the invention, water and sucrose.In the preparation process of beverage, can first to sugarcane polysaccharide extraction liquid provided by the invention, concentrate, obtain sugarcane polysaccharide and extract concentrate, by percentage to the quality, the content that gained sugarcane polysaccharide extracts sugarcane polysaccharide in concentrate is 0.22%～1.18%; In this preparation method of beverage, beverage provided by the invention is comprised of sugarcane polysaccharide extraction concentrate, the water of 53.25～54.75 weight portions and the sucrose of 5.25～6.75 weight portions of 15 weight portions;
The preparation method of this sugarcane polysaccharide extraction liquid comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
In some embodiments of the invention, the raw material of this beverage is sugarcane polysaccharide extraction liquid provided by the invention, water and sucrose.In the preparation process of beverage, can first to sugarcane polysaccharide extraction liquid provided by the invention, concentrate, obtain sugarcane polysaccharide and extract concentrate, by percentage to the quality, the content that gained sugarcane polysaccharide extracts sugarcane polysaccharide in concentrate is 0.22%～1.18%; In this preparation method of beverage, beverage provided by the invention is comprised of sugarcane polysaccharide extraction concentrate, the water of 36 weight portions and the sucrose of 4 weight portions of 10 weight portions;
The preparation method of this sugarcane polysaccharide extraction liquid comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
The invention provides a kind of preparation method and application of sugarcane polysaccharide extraction liquid.The preparation method of this sugarcane polysaccharide extraction liquid is: collect sugarcane top and/or sugarcane toppers, obtain the first product; This first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.Preparation method's step provided by the invention is few, preparation technology is simple, takes full advantage of sugarcane top and sugarcane toppers resource, and contains abundant sugarcane polysaccharide in gained sugarcane polysaccharide extraction liquid, can be applied to preparation containing the beverage of sugarcane polysaccharide.Beverage provided by the invention comprises the sugarcane polysaccharide extraction liquid that preparation method provided by the invention makes, and this makes both to have contained appropriate sugarcane polysaccharide in this beverage, has again the distinctive local flavor of sugarcane, has utilized cleverly sugarcane polysaccharide extraction liquid.
The specific embodiment
The invention discloses a kind of preparation method and application of sugarcane polysaccharide extraction liquid.Those skilled in the art can implement this preparation method with reference to this paper content, obtain sugarcane polysaccharide extraction liquid, realize its application.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in the present invention.Preparation method of the present invention and application are described by preferred embodiment, related personnel obviously can change this paper preparation method and application or suitably change and combination within not departing from content of the present invention, spirit and scope, realizes and apply the technology of the present invention.
In the preparation method and application of a kind of sugarcane polysaccharide extraction liquid provided by the invention, reagent used and raw material all can be buied by market.
In order to make those skilled in the art can understand better technical scheme of the present invention, below in conjunction with embodiment, further set forth the present invention:
The preparation of embodiment 1 sugarcane polysaccharide extraction liquid
The fresh sugarcane top of gathering, puts it in dehydrating drier and is dried to obtain the dry sugarcane top of 5kg, with chaffcutter, is cut into 1-2 centimetre fragment, collects fragment; Gained fragment is dropped into ultrasonic cleaner, and water cleans 2-3 time, collects the sugarcane top cleaning up; Sugarcane top is put into extraction pot, add the water of 75kg, stir, be heated to 80 ℃, insulation 120min, extracts.After extraction, gained mixture is carried out centrifugal, collect centrifugate, obtain sugarcane polysaccharide extraction liquid 75kg.Gained sugarcane polysaccharide extraction liquid is poured in vacuum concentration pot and concentrated 10 times, obtain sugarcane polysaccharide and extract concentrate 7.5kg.
Adopt following methods, structure to sugarcane polysaccharide in the sugarcane polysaccharide extraction liquid of the present embodiment gained is identified, and detect its content: the sugarcane polysaccharide of 7.5kg gained is extracted to concentrated 40 times of concentrate, to 95% ethanol that adds 10 times of volumes in gained concentrate, static spending the night, centrifuging and taking precipitation, weighs after drying precipitate, obtains the thick polysaccharide of 100g sugarcane.Adopt Molish reaction method to carry out the evaluation of sugarcane polysaccharide, the positive reaction of qualification result, shows that this product is glucide.Adopt phenolsulfuric acid reagent to measure the content of sugarcane polysaccharide in the thick polysaccharide of sugarcane, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result be sugarcane polysaccharide account for the thick polyoses content of sugarcane 60%.By percentage to the quality, in the sugarcane polysaccharide extraction liquid of the present embodiment gained, the content of sugarcane polysaccharide is 0.08%.
The recovery rate of the present embodiment sugarcane polysaccharide calculates in accordance with the following methods: quality * 100 of the quality/raw material of the sugarcane polysaccharide that recovery rate %=extracts.The recovery rate that adopts above-mentioned computational methods to obtain the sugarcane polysaccharide of preparation method's gained that the present embodiment provides is 1.2%.
The preparation of embodiment 2 sugarcane polysaccharide extraction liquids
Get the sugarcane top 5kg drying, with chaffcutter, cut into pieces; Gained fragment is dropped into ultrasonic cleaner, and water cleans 2-3 time, collects the sugarcane top cleaning up; Sugarcane top is put into extraction pot, add the water of 100kg, stir, soak 5h and be heated to 100 ℃ afterwards, insulation 60min, extracts.After extraction, gained mixture is carried out to press filtration, collect filtrate, obtain sugarcane polysaccharide extraction liquid 100kg.
Adopt following methods, sugarcane polysaccharide in the sugarcane polysaccharide extraction liquid of the present embodiment gained is carried out to content detection: by concentrated 320 times of the sugarcane polysaccharide extraction liquid of 100kg gained, to 95% ethanol that adds 10 times of volumes in gained concentrate, static spending the night, centrifuging and taking precipitation, after drying precipitate, weigh, obtain the thick polysaccharide of 90g sugarcane.Adopt Molish reaction method to carry out the evaluation of sugarcane polysaccharide, the positive reaction of qualification result, shows that this product is glucide.Adopt phenolsulfuric acid reagent to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result be sugarcane polysaccharide account for the thick polyoses content of sugarcane 52%.By percentage to the quality, in the sugarcane polysaccharide extraction liquid of the present embodiment gained, the content of sugarcane polysaccharide is 0.047%.
The recovery rate of the present embodiment sugarcane polysaccharide calculates in accordance with the following methods: quality * 100 of the quality/raw material of the sugarcane polysaccharide that recovery rate/%=extracts.The recovery rate that adopts above-mentioned computational methods to obtain the sugarcane polysaccharide of preparation method's gained that the present embodiment provides is 0.94%.
The preparation of embodiment 3 sugarcane polysaccharide extraction liquids
Sugarcane top and the new sugarcane toppers of plucking of getting new harvesting, dry naturally, obtains sugarcane top and the dry sugarcane toppers of 4kg that 1kg is dry, with chaffcutter, cuts into pieces; Gained fragment is dropped into ultrasonic cleaner, and water cleans 2-3 time, collects the sugarcane top and the sugarcane toppers that clean up; Sugarcane top and sugarcane toppers are put into extraction pot, add the water of 125kg, stir, soak 3h and be heated to 70 ℃ afterwards, insulation 150min, extracts.After extraction, gained mixture is carried out centrifugal, collect centrifugate, obtain sugarcane polysaccharide extraction liquid 125kg.
Adopt following methods, sugarcane polysaccharide in the sugarcane polysaccharide extraction liquid of the present embodiment gained is carried out to content detection: by concentrated 400 times of the sugarcane polysaccharide extraction liquid of 125kg gained, to 95% ethanol that adds 10 times of volumes in gained concentrate, static spending the night, centrifuging and taking precipitation, after drying precipitate, weigh, obtain the thick polysaccharide of 85g sugarcane.Adopt Molish reaction method to carry out the evaluation of sugarcane polysaccharide, the positive reaction of qualification result, shows that this product is glucide.Adopt phenolsulfuric acid reagent to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result be sugarcane polysaccharide account for the thick polyoses content of sugarcane 48.5%.By percentage to the quality, in the sugarcane polysaccharide extraction liquid of the present embodiment gained, the content of sugarcane polysaccharide is 0.033%.
The recovery rate of the present embodiment sugarcane polysaccharide calculates in accordance with the following methods: quality * 100 of the quality/raw material of the sugarcane polysaccharide that recovery rate/%=extracts.The recovery rate that adopts above-mentioned computational methods to obtain the sugarcane polysaccharide of preparation method's gained that the present embodiment provides is 0.82%.
The preparation of embodiment 4 sugarcane polysaccharide extraction liquids
Get fresh sugarcane toppers, put it in dehydrating drier and be dried, obtain the sugarcane toppers that 5kg is dry, with chaffcutter, cut into pieces; Gained fragment is dropped into ultrasonic cleaner, and water cleans 2-3 time, collects the sugarcane toppers cleaning up; Sugarcane toppers is put into extraction pot, add the water of 50kg, stir, soak 1h and be heated to 85 ℃ afterwards, insulation 120min, extracts.After extraction, gained mixture is centrifugal, collect centrifugate, obtain sugarcane polysaccharide extraction liquid 50kg.Gained sugarcane polysaccharide extraction liquid is poured in vacuum concentration pot and concentrated 10 times, obtain sugarcane polysaccharide and extract concentrate 5kg.
Adopt following methods, sugarcane polysaccharide in the sugarcane polysaccharide extraction liquid of the present embodiment gained is carried out to content detection: the sugarcane polysaccharide of 5kg gained is extracted to concentrated 40 times of concentrate, to 95% ethanol that adds 10 times of volumes in gained concentrate, static spending the night, centrifuging and taking precipitation, after drying precipitate, weigh, obtain the thick polysaccharide of 105g sugarcane.Adopt Molish reaction method to carry out the evaluation of sugarcane polysaccharide, the positive reaction of qualification result, shows that this product is glucide.Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result be sugarcane polysaccharide account for the thick polyoses content of sugarcane 56%.By percentage to the quality, in the sugarcane polysaccharide extraction liquid of the present embodiment gained, the content of sugarcane polysaccharide is 0.118%.
The recovery rate of the present embodiment sugarcane polysaccharide calculates in accordance with the following methods: quality * 100 of the quality/raw material of the sugarcane polysaccharide that recovery rate/%=extracts.The recovery rate that adopts above-mentioned computational methods to obtain the sugarcane polysaccharide of preparation method's gained that the present embodiment provides is 1.17%.
The preparation of embodiment 5 sugarcane polysaccharide extraction liquids
Get the sugarcane top 5kg drying, with chaffcutter, cut into pieces; Gained fragment is dropped into ultrasonic cleaner, and water cleans 2-3 time, collects the sugarcane top cleaning up; Sugarcane top is put into extraction pot, add the water of 250kg, stir, soak 2h and be heated to 100 ℃ afterwards, insulation 60min, extracts.After extraction, gained mixture is filtered, collect filtrate, obtain sugarcane polysaccharide extraction liquid 250kg.Gained sugarcane polysaccharide extraction liquid is poured in vacuum concentration pot and concentrated, concentrated 10 times, obtain sugarcane polysaccharide and extract concentrate 25kg.
Adopt following methods, sugarcane polyoses content in the sugarcane polysaccharide extraction liquid of the present embodiment gained is detected: the sugarcane polysaccharide of 25kg gained is extracted to concentrated 40 times of concentrate, to 95% ethanol that adds 10 times of volumes in gained concentrate, static spending the night, centrifuging and taking precipitation, after drying precipitate, weigh, obtain the thick polysaccharide of 106g sugarcane.Adopt Molish reaction method to carry out the evaluation of sugarcane polysaccharide, the positive reaction of qualification result, shows that this product is glucide.Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result be sugarcane polysaccharide account for the thick polyoses content of sugarcane 52%.By percentage to the quality, in the sugarcane polysaccharide extraction liquid of the present embodiment gained, the content of sugarcane polysaccharide is 0.022%.
In the present embodiment, the recovery rate of sugarcane polysaccharide calculates in accordance with the following methods: quality * 100 of the quality/raw material of the sugarcane polysaccharide that recovery rate/%=extracts.The recovery rate that adopts above-mentioned computational methods to obtain the sugarcane polysaccharide of preparation method's gained that the present embodiment provides is 1.10%.
The preparation of embodiment 6 beverages
The sugarcane polysaccharide that the preparation method that getting provides according to embodiment 1 makes extracts concentrate 1000g, water 3600g, edible sucrose 400g, mix, after adopting autoclave techniques sterilizing, carry out filtration under diminished pressure, the beverage containing sugarcane polysaccharide is packed and obtained to filtrate, according to the packing of 500g/ bottle.
Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result obtains the sugarcane polysaccharide that contains 800mg in every 500g beverage, and by percentage to the quality, in the beverage that the present embodiment provides, the content of sugarcane polysaccharide is 0.16%.Meanwhile, in every 500g beverage, also contain the sucrose of 40g, the heat of 160 kilocalories.
The preparation of embodiment 7 beverages
The sugarcane polysaccharide extraction liquid 10kg that the preparation method that getting provides according to embodiment 2 makes, be poured in vacuum concentration pot and concentrate 10 times, obtain sugarcane polysaccharide and extract concentrate 1000g, add wherein the water of 3600g, the edible sucrose of 400g, mix, after adopting the sterilizing of membrane filtration sterilization technique, the beverage containing sugarcane polysaccharide is packed and obtained to filtrate, according to the packing of 200g/ bottle.
Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result obtains the sugarcane polysaccharide that contains 468mg in every 500g beverage, and by percentage to the quality, in the beverage that the present embodiment provides, the content of sugarcane polysaccharide is 0.094%.Meanwhile, in every 500g beverage, also contain the sucrose of 40g, the heat of 160 kilocalories.
The preparation of embodiment 8 beverages
The sugarcane polysaccharide extraction liquid 10kg that the preparation method that getting provides according to embodiment 3 makes, be poured in vacuum concentration pot and concentrate 10 times, obtain sugarcane polysaccharide and extract concentrate 1000g, add wherein the water of 3550g, the edible sucrose of 450g, mix, after adopting autoclave techniques sterilizing, carry out filtration under diminished pressure, the beverage containing sugarcane polysaccharide is packed and obtained to filtrate, according to the packing of 1000g/ bottle.
Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result obtains the sugarcane polysaccharide that contains 330mg in every 500g beverage, and by percentage to the quality, in the beverage that the present embodiment provides, the content of sugarcane polysaccharide is 0.066%.Meanwhile, in every 500g beverage, also contain the sucrose of 45g, the heat of 180 kilocalories.
The preparation of embodiment 9 beverages
The sugarcane polysaccharide that the preparation method that getting provides according to embodiment 4 makes extracts concentrate 1000g, water 3600g, edible sucrose 400g, mix, after adopting autoclave techniques sterilizing, carry out filtration under diminished pressure, the beverage containing sugarcane polysaccharide is packed and obtained to filtrate, according to the packing of 500g/ bottle.
Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result obtains the sugarcane polysaccharide that contains 1180mg in every 500g beverage, and by percentage to the quality, in the beverage that the present embodiment provides, the content of sugarcane polysaccharide is 0.236%.Meanwhile, in every 500g beverage, also contain the sucrose of 40g, the heat of 160 kilocalories.
The preparation of embodiment 10 beverages
The sugarcane polysaccharide that the preparation method that getting provides according to embodiment 5 makes extracts concentrate 1000g, water 3650g, edible sucrose 350g, mix, adopt autoclave techniques to carry out sterilizing, carry out afterwards filtration under diminished pressure, the beverage containing sugarcane polysaccharide is packed and obtained to filtrate, according to the packing of 100g/ bottle.
Adopt reducing process to measure the wherein content of sugarcane polysaccharide, the measurement of the polysaccharide content method that the Measuring The Content of Polysaccharides In Radix Cynanchum Bungei By The Method of Phenol-vitril that concrete detection method is delivered on Journal of Shandong agri.Univ (natural science edition) with reference to (2004) such as Koryo monarchs is reported.Testing result obtains the sugarcane polysaccharide that contains 220mg in every 500g beverage, and by percentage to the quality, in the beverage that the present embodiment provides, the content of sugarcane polysaccharide is 0.044%.Meanwhile, in every 500g beverage, also contain the sucrose of 35g, the heat of 140 kilocalories.
Smell and the local flavor official of embodiment 11 beverages can evaluate
In national northwest, southwest, northeast, North China, the southeast, Central China, choose at random respectively 1200 altogether of each age group, every profession and trade personages, according to age, engaged in trade, be divided into 40 groups, every group of 30 surveyees, beverage prepared by the preparation method that the embodiment of the present invention 6 to 10 is provided carries out smell and local flavor official can evaluate, sense evaluation criterion is in Table 1, evaluation result in Table 2, table 3.
Table 1 official can evaluation criterion
The smell evaluation result of table 2 beverage
The flavor evaluation result of table 3 beverage
By investigation result, being shown, altogether in 1200 surveyees, is 91.7% to the smell satisfaction rate of beverage provided by the invention from national northwest, southwest, northeast, North China, the southeast, Central China's each age group, every profession and trade personage, and local flavor satisfaction rate is 95.0%.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a preparation method for sugarcane polysaccharide extraction liquid, is characterized in that, comprises the following steps:
Collect sugarcane top and/or sugarcane toppers, obtain the first product;
Described the first product is mixed 1:10～50 in mass ratio with water, at 70 ℃～100 ℃ temperature, extract 60min～150min, collect extract, obtain.
2. preparation method according to claim 1, is characterized in that, the mass ratio of described the first product and water is 1:15～25.
3. preparation method according to claim 1, is characterized in that, the mass ratio of described the first product and water is 1:20.
4. preparation method according to claim 1, is characterized in that, the temperature of described extraction is 85 ℃.
5. preparation method according to claim 1, is characterized in that, the time of described extraction is 90min～120min.
6. preparation method according to claim 1, is characterized in that, the time of described extraction is 120min.
7. preparation method according to claim 1, is characterized in that, after described collection extract, also comprises concentrated step.
8. the sugarcane polysaccharide extraction liquid that preparation method makes as claimed in any of claims 1 to 7 in one of claims contains the application in the beverage of sugarcane polysaccharide in preparation.
9. a beverage, is characterized in that, it comprises acceptable food additives on preparation method makes as described in any one in claim 1 to 7 sugarcane polysaccharide extraction liquid and beverage.
10. beverage according to claim 9, is characterized in that, by percentage to the quality, the content of its sugarcane polysaccharide is 0.044%～0.236%.
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| CN101549117A (en) * | 2009-05-13 | 2009-10-07 | 广西中医学院 | Bagasse (leaf) extract with anti-tumor effect and its application |
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101549117A (en) * | 2009-05-13 | 2009-10-07 | 广西中医学院 | Bagasse (leaf) extract with anti-tumor effect and its application |
| CN102911286A (en) * | 2012-11-22 | 2013-02-06 | 广西中医药大学 | Preparation method of sugarcane leaf polysaccharides |
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