CN105169371A - Composition, applications thereof and moisture retention preparation - Google Patents
Composition, applications thereof and moisture retention preparation Download PDFInfo
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- CN105169371A CN105169371A CN201510500408.XA CN201510500408A CN105169371A CN 105169371 A CN105169371 A CN 105169371A CN 201510500408 A CN201510500408 A CN 201510500408A CN 105169371 A CN105169371 A CN 105169371A
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Abstract
The invention relates to the biological technology field, and especially relates to a composition, applications thereof and a moisture retention preparation. The composition comprises mushrooms or mushroom extract products, cucumbers or cucumber extract products, collagen Mushroom polypeptides are employed as a characteristic raw material, natural cucumber extracting solution, collagen polypeptides and aloe extracting solution are added for blending, subpackaging is carried out and products are obtained. The provided product is suitable for people of all ages, and has obvious functions of moisture retention, whitening and the like.
Description
Technical field
The present invention relates to biological technical field, particularly composition and use thereof, moisturising preparations.
Background technology
Lentinus Edodes is second-biggest-in-the-world edible fungi, at China's title have " delicacy from mountain " among the people.According to analysis, the edible part of dried thin mushroom accounts for 72%, and every 100g eats in part containing albumen 13g.Lentinus Edodes contains lentinan, champignon polypeptide isoreactivity composition, there is very high medical value, domestic and international many experts and scholars focus on lentinan research very much, and in order to pursue highly purified lentinan, champignon polypeptide is often dropped waste in the process preparing lentinan.
Along with the rising of people's quality of life, people more and more pay attention to the Study and appliance of pure natural moisturizer.Champignon polypeptide system extraction separation and purification from fungus mushroom fruiting body obtain polypeptide.Peptide chain by aspartic acid, histidine etc. 18 seed amino acid form.Champignon polypeptide is the primary bioactivity material in Lentinus Edodes, does not also study the moisture-keeping functions of champignon polypeptide at present.
The polypeptide moisturizing research appeared in the newspapers at present from Eriocheir sinensis, shellfish, Sargassum or the source of fish, extracts polypeptide products carry out moisturizing, and product cost is single, and moistening effect is undesirable.
Therefore, a kind of moisture-keeping composition containing champignon polypeptide is provided to have important practical significance.
Summary of the invention
In view of this, the invention provides a kind of composition and use thereof, moisturising preparations.This product suits the taste of both old and young, and has obviously moisturizing, Bearberry Extract.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides a kind of compositions, comprise Lentinus Edodes or Lentinus Edodes extract, Fructus Cucumidis sativi or Fructus Cucumidis sativi extract, collagen protein, Fructus Cucumidis sativi or Fructus Cucumidis sativi extract.
In specific embodiments more of the present invention, compositions provided by the invention is made up of the raw material of following weight portion:
Containing a large amount of hydrophilic groups in champignon polypeptide chain, for the surface of hydrone, to skin, there is good moisture retention, and its moisture-keeping function is not by the impact that environment (temperature, humidity) changes.It is desirable wetting agent.
In specific embodiments more of the present invention, described in compositions provided by the invention, the preparation method of Lentinus Edodes extract comprises the steps:
Step 1: get Lentinus Edodes and pulverize, after mixing with water, heating, cooling;
Step 2: get the product of step 1 after ultrasonic tear reason, adjust ph is 8.5, adds protease, enzymolysis;
Step 3: the enzymatic hydrolysate getting step 2, adjust ph is 5.0, adds protease, enzymolysis, enzyme-deactivating, cooling;
Step 4: the pH value of the enzymatic hydrolysate of regulating step 3 is 7.0, heating, cooling, centrifugal filtration, collects filtrate;
Step 5: add decolorizing with activated carbon in the filtrate that step 4 obtains, filter, collect filtrate; Get described filtrate through ultrafilter membrane ultrafiltration, collect ultrafiltrate, obtain champignon polypeptide extract.
In specific embodiments more of the present invention, described in the preparation method step 1 of Lentinus Edodes extract described in compositions provided by the invention, the mass ratio of Lentinus Edodes and water is 1:10.
In specific embodiments more of the present invention, described in the preparation method step 1 of Lentinus Edodes extract described in compositions provided by the invention, the temperature of heating is 95 DEG C, and the time of described heating is 90min.
In specific embodiments more of the present invention, described in the preparation method step 1 of Lentinus Edodes extract described in compositions provided by the invention, be cooled to 15 ~ 35 DEG C.
In specific embodiments more of the present invention, described in the preparation method step 2 of Lentinus Edodes extract described in compositions provided by the invention, protease is alkaline protease, trypsin or papain.
In the preparation method step 2 of Lentinus Edodes extract described in compositions provided by the invention, every kg dried thin mushroom adds the described protease of 5.0 million international units Rate activity.
Described in the preparation method step 2 of Lentinus Edodes extract described in compositions provided by the invention, the temperature of enzymolysis is 50 DEG C, and the time of described enzymolysis is 180min.
In specific embodiments more of the present invention, described in the preparation method step 3 of Lentinus Edodes extract described in compositions provided by the invention, protease is alkaline protease, trypsin or papain.
In specific embodiments more of the present invention, in the preparation method step 3 of Lentinus Edodes extract described in compositions provided by the invention, every kg dried thin mushroom adds the described protease of 5.0 million international units Rate activity.
In specific embodiments more of the present invention, described in the preparation method step 3 of Lentinus Edodes extract described in compositions provided by the invention, the temperature of enzymolysis is 50 DEG C, and the time of described enzymolysis is 180min.
In specific embodiments more of the present invention, described in the preparation method step 3 of Lentinus Edodes extract described in compositions provided by the invention, be cooled to 15 ~ 35 DEG C.
In specific embodiments more of the present invention, described in the preparation method step 4 of Lentinus Edodes extract described in compositions provided by the invention, the temperature of heating is 95 DEG C, and the time of described heating is 180min.
In specific embodiments more of the present invention, described in the preparation method step 4 of Lentinus Edodes extract described in compositions provided by the invention, be cooled to 15 ~ 35 DEG C.
In specific embodiments more of the present invention, centrifugally in the preparation method step 4 of Lentinus Edodes extract described in compositions provided by the invention be specially 8000 turns, centrifugal 8 minutes.
In specific embodiments more of the present invention, in the preparation method step 5 of Lentinus Edodes extract described in compositions provided by the invention, the volume ratio of the filtrate that step 4 obtains and activated carbon is 100:0.5.
In specific embodiments more of the present invention, described in the preparation method step 5 of Lentinus Edodes extract described in compositions provided by the invention, the specification of ultrafilter membrane is 5000 dalton.
In specific embodiments more of the present invention, described in compositions provided by the invention, the preparation method of Fructus Cucumidis sativi extract comprises the steps:
Step 1: get Fructus Cucumidis sativi and squeeze the juice, collects Sucus Cucumidis sativi; Get Fructus Cucumidis sativi slag to mix with water, heating, cooling, adds plant composite hydrolytic enzyme, enzymolysis, enzyme-deactivating, filters, and collects filtrate;
Step 2: the Sucus Cucumidis sativi of combining step 1 and filtrate, add carrageenan, mixing, leave standstill, collected by centrifugation supernatant, obtains Fructus Cucumidis sativi extract.
In specific embodiments more of the present invention, in the preparation method step 1 of Fructus Cucumidis sativi extract described in compositions provided by the invention, the mass ratio of Fructus Cucumidis sativi slag and water is 1:5.
In specific embodiments more of the present invention, described in the preparation method step 1 of Fructus Cucumidis sativi extract described in compositions provided by the invention, the temperature of heating is 80 DEG C, and the time of described heating is 1h.
In specific embodiments more of the present invention, described in the preparation method step 1 of Fructus Cucumidis sativi extract described in compositions provided by the invention, be cooled to 15 ~ 35 DEG C.
In specific embodiments more of the present invention, in the preparation method step 1 of Fructus Cucumidis sativi extract described in compositions provided by the invention, every kg Fructus Cucumidis sativi slag adds the described plant composite hydrolytic enzyme of 3.0 million international units Rate activity.
In specific embodiments more of the present invention, described in the preparation method step 1 of Fructus Cucumidis sativi extract described in compositions provided by the invention, the temperature of enzymolysis is 50 DEG C, and the time of described enzymolysis is 1h.
In specific embodiments more of the present invention, the mixed liquor of Sucus Cucumidis sativi and filtrate described in the preparation method step 2 of Fructus Cucumidis sativi extract described in compositions provided by the invention and the mass ratio of carrageenan are 10000:5.
In specific embodiments more of the present invention, leaving standstill in the preparation method step 2 of Fructus Cucumidis sativi extract described in compositions provided by the invention is 4 DEG C of placements 2 hours.
In specific embodiments more of the present invention, described in compositions provided by the invention, the preparation method of collagen protein comprises the steps:
Step 1: get fish skin or fish scale, mix with water, micronizing, sieve;
Step 2: the product adjust ph of getting step 1 is 8.5, adds alkaline protease, enzymolysis, enzyme-deactivating, cooling;
Step 3: add decolorizing with activated carbon, collecting by filtration filtrate in the enzymatic hydrolysate that step 2 obtains, adjust ph is 6.0, to obtain final product.
In specific embodiments more of the present invention, described in the preparation method step 1 of collagen protein described in compositions provided by the invention, the mass ratio of fish skin or fish scale and water is 1:8.
In specific embodiments more of the present invention, sieving in the preparation method step 1 of collagen protein described in compositions provided by the invention is 200 orders.
In specific embodiments more of the present invention, described in the preparation method step 2 of collagen protein described in compositions provided by the invention, the solution of adjust ph is the alkali liquor of 10.0mol/L.
In specific embodiments more of the present invention, described in the preparation method step 2 of collagen protein described in compositions provided by the invention, the mass ratio of fish skin or fish scale and described alkaline protease is 100:0.1.
In specific embodiments more of the present invention, described in the preparation method step 2 of collagen protein described in compositions provided by the invention, the temperature of enzymolysis is 55 DEG C, and the time of described enzymolysis is 120min.
In specific embodiments more of the present invention, the temperature of enzyme-deactivating described in the preparation method step 2 of collagen protein described in compositions provided by the invention is 90 DEG C ~ 100 DEG C, and the time of described enzyme-deactivating is 10min.
In specific embodiments more of the present invention, described in the preparation method step 2 of collagen protein described in compositions provided by the invention, be cooled to 60 DEG C.
In specific embodiments more of the present invention, in the preparation method step 3 of collagen protein described in compositions provided by the invention, the mass ratio of the enzymatic hydrolysate that step 2 obtains and activated carbon is 100:0.5.
In specific embodiments more of the present invention, in the preparation method step 3 of collagen protein described in compositions provided by the invention, the temperature of decolouring is 60 DEG C, and the time of decolouring is 60min.
In specific embodiments more of the present invention, in the preparation method step 3 of collagen protein described in compositions provided by the invention, the solution of adjust ph is the citric acid solution of 10.0mol/L.
In specific embodiments more of the present invention, described in compositions provided by the invention, the preparation method of Aloe extract comprises the steps:
Step 1: get Folium Aloe, grinds pulping, adds plant composite hydrolytic enzyme, enzymolysis, enzyme-deactivating, cooling;
Step 2: the enzymatic hydrolysate getting step 1 adds decolorizing with activated carbon, centrifugal filtration, collects filtrate, obtains Aloe extract.
In specific embodiments more of the present invention, in the preparation method step 1 of Aloe extract described in compositions provided by the invention, every kg Folium Aloe adds the described plant composite hydrolytic enzyme of 3.0 million international units Rate activity.
In specific embodiments more of the present invention, described in the preparation method step 1 of Aloe extract described in compositions provided by the invention, the temperature of enzymolysis is 55 DEG C, and the time of described enzymolysis is 3h.
In specific embodiments more of the present invention, described in the preparation method step 1 of Aloe extract described in compositions provided by the invention, the temperature of enzyme-deactivating is 90 DEG C, and the time of described enzyme-deactivating is 10min.
In specific embodiments more of the present invention, described in the preparation method step 1 of Aloe extract described in compositions provided by the invention, be cooled to 60 DEG C.
In specific embodiments more of the present invention, in the preparation method step 2 of Aloe extract described in compositions provided by the invention, the mass ratio of the enzymatic hydrolysate that step 1 obtains and activated carbon is 100:0.5.
In specific embodiments more of the present invention, in the preparation method step 2 of Aloe extract described in compositions provided by the invention, the temperature of decolouring is 60 DEG C, and the time of decolouring is 30min.
In specific embodiments more of the present invention, centrifugally in the preparation method step 2 of Aloe extract described in compositions provided by the invention be specially 8000 turns, centrifugal 8 minutes.
Present invention also offers described compositions for the preparation of the purposes in the medicine of moisturizing, whitening, health product or cosmetics.
Present invention also offers a kind of moisturising preparations, comprise described compositions.
Concrete, the invention provides a kind of pure natural champignon polypeptide wetting agent, its feature raw material is champignon polypeptide, cucumber extract, collagen polypeptide, Aloe extraction solution, and product is sparkling and crystal-clear bright liquid.
Novel coconut water beverage of the present invention take champignon polypeptide as primary raw material, is below the preparation method of pure natural champignon polypeptide wetting agent:
Champignon polypeptide 30-50%, cucumber extract 15-20%, collagen polypeptide 15-20%, Aloe extraction solution 20-30%, product is sparkling and crystal-clear bright liquid.
Described champignon polypeptide preparation method is as follows:
1., by dried thin mushroom sporophore pulverize, add the water of 10 times of weight, stir, be heated to 95 DEG C, maintain 90min, be cooled to room temperature;
2., step 1. aqueous solution carry out ultrasonic Treatment, adjust pH to 8.5, add the protease of 5.0 million international units Rate activity by every kg dried thin mushroom, in 50 DEG C hydrolysis 180min;
3., regulating step 2. hydrolyzed solution pH value to 5.0, add the protease of 5.0 million international units Rate activity by every kg dried thin mushroom, 50 DEG C hydrolysis 360min; Enzyme-deactivating, is down to room temperature;
4., the hydrolyzed solution pH value to 7.0 of pacing suddenly 3. after deactivation, be heated to 95 DEG C, keep 180min, be down to room temperature, centrifugal filtration, collect filtrate;
5., be 0.5% add decolorizing with activated carbon by carbon weight and filtrate volume ratio in step 4. filtrate, filter, filtrate is faint yellow supernatant liquid; Filtrate carries out ultrafiltration through 5000 daltonian ultrafilter membranes, and collect ultrafiltrate, obtaining champignon polypeptide extracting solution, is basic material of the present invention.
Described cucumber extract preparation method is as follows:
1., get fresh cucumber, juice extractor is squeezed the juice, and collects Sucus Cucumidis sativi, what Fructus Cucumidis sativi slag added 5 times amount goes dried up stirring, and is heated to 80 DEG C, keeps 1 hour, be cooled to room temperature, the plant composite hydrolytic enzyme adding 3.0 million international units Rate activity by every kg Fructus Cucumidis sativi slag carries out enzymolysis, and hydrolysis temperature is 50 DEG C, enzymolysis time 1 hour, enzyme-deactivating is carried out after enzymolysis terminates, filter, get filtered solution, for subsequent use.
2., merge Sucus Cucumidis sativi and filtered solution, add the carrageenan of liquid weight 5/10000ths, fully stir evenly, place 2 hours for 4 DEG C, centrifugal, get clear liquor, be cucumber extract.
Described collagen polypeptide preparation method is as follows:
1., fetch water and produce processing fent fish skin and scale, clean, add the purified water of fish skin and scale weight 8 times, micronizing with tap water, after pulverizing, fish skin and scale can cross 200 mesh sieves, for subsequent use;
2., by the alkali liquor adjust ph to 8.5 of 10.0mol/L, maintain pH value 8.5, add alkaline protease (adding fish skin and scale weight 0.1% alkaline protease) enzymolysis 120min, hydrolysis temperature is 55 DEG C.Heat after enzymolysis terminates to more than 90 DEG C 10min, carry out enzyme-deactivating, be cooled to 60 DEG C, for subsequent use;
3., add 0.5% decolorizing with activated carbon, holding temperature 60 DEG C of 60min, stir decolouring, filter, adjust pH value to 6.0 with the citric acid solution of 10.0mol/L, be collagen polypeptide;
Described Aloe extraction solution preparation method is as follows:
Get fresh aloe leaf, clean peeling, grind pulping, the plant composite hydrolytic enzyme adding 3.0 million international units Rate activity by every kg fresh aloe leaf carries out enzymolysis, hydrolysis temperature is 55 DEG C, enzymolysis time is 3 hours, and enzymolysis terminates post-heating to 90 DEG C and carries out enzyme-deactivating in 10 minutes, lowers the temperature 60 DEG C, added for 0.5% decolorizing with activated carbon half an hour, centrifugal filtration, gets supernatant liquid, is Aloe extraction solution.
Present invention also offers pure natural champignon polypeptide wetting agent at moisture-keeping functions.
The invention provides a kind of compositions, pure natural wetting agent, its preparation method and the moisture-keeping functions thereof that are feature raw material with champignon polypeptide, cucumber extract, collagen polypeptide and Aloe extraction solution, relate to formula and the preparation method of pure natural wetting agent.Pure natural wetting agent is feature raw material with champignon polypeptide, adds the allotment of natural cucumber extracting solution, collagen polypeptide and Aloe extraction solution, subpackage and obtaining.This product suits the taste of both old and young, and has obviously moisturizing, Bearberry Extract.
Detailed description of the invention
The invention discloses a kind of composition and use thereof, moisturising preparations, those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope methods and applications as herein described are changed or suitably change with combination, realize and apply the technology of the present invention.
In composition and use thereof provided by the invention, moisturising preparations, raw materials used and reagent all can be buied by market.
Below in conjunction with embodiment, set forth the present invention further:
The preparation of embodiment 1 Lentinus Edodes extract
1., by dried thin mushroom sporophore pulverize, add the water of 10 times of weight, stir, be heated to 95 DEG C, maintain 90min, be cooled to room temperature;
2., step 1. aqueous solution carry out ultrasonic Treatment, adjust pH to 8.5, add the protease of 5.0 million international units Rate activity by every kg dried thin mushroom, in 50 DEG C hydrolysis 180min;
3., regulating step 2. hydrolyzed solution pH value to 5.0, add the protease (alkaline protease, trypsin or papain) of 5.0 million international units Rate activity by every kg dried thin mushroom, 50 DEG C hydrolysis 360min; Enzyme-deactivating, is down to room temperature;
4., the hydrolyzed solution pH value to 7.0 of pacing suddenly 3. after deactivation, be heated to 95 DEG C, keep 180min, be down to room temperature, centrifugal filtration, collect filtrate;
5., be 0.5% add decolorizing with activated carbon by carbon weight and filtrate volume ratio in step 4. filtrate, filter, filtrate is faint yellow supernatant liquid; Filtrate carries out ultrafiltration through 5000 daltonian ultrafilter membranes, and collect ultrafiltrate, obtaining champignon polypeptide extracting solution, is basic material of the present invention.
The preparation of embodiment 2 Fructus Cucumidis sativi extract
1., get fresh cucumber, juice extractor is squeezed the juice, and collects Sucus Cucumidis sativi, what Fructus Cucumidis sativi slag added 5 times amount goes dried up stirring, and is heated to 80 DEG C, keeps 1 hour, be cooled to room temperature, the plant composite hydrolytic enzyme adding 3.0 million international units Rate activity by every kg Fructus Cucumidis sativi slag carries out enzymolysis, and hydrolysis temperature is 50 DEG C, enzymolysis time 1 hour, enzyme-deactivating is carried out after enzymolysis terminates, filter, get filtered solution, for subsequent use.
2., merge Sucus Cucumidis sativi and filtered solution, add the carrageenan of liquid weight 5/10000ths, fully stir evenly, place 2 hours for 4 DEG C, centrifugal, get clear liquor, be cucumber extract.
The preparation of embodiment 3 collagen polypeptide
1., fetch water and produce processing fent fish skin and scale, clean, add the purified water of fish skin and scale weight 8 times, micronizing with tap water, after pulverizing, fish skin and scale can cross 200 mesh sieves, for subsequent use;
2., by the alkali liquor adjust ph to 8.5 of 10.0mol/L, maintain pH value 8.5, add alkaline protease (adding fish skin and scale weight 0.1% alkaline protease) enzymolysis 120min, hydrolysis temperature is 55 DEG C.Heat to 90 DEG C ~ 100 DEG C after enzymolysis terminates, 10min, carries out enzyme-deactivating, is cooled to 60 DEG C, for subsequent use;
3., add 0.5% decolorizing with activated carbon, holding temperature 60 DEG C of 60min, stir decolouring, filter, adjust pH value to 6.0 with the citric acid solution of 10.0mol/L, be collagen polypeptide.
The preparation of embodiment 4 Aloe extract
Get fresh aloe leaf, clean peeling, grind pulping, the plant composite hydrolytic enzyme adding 3.0 million international units Rate activity by every kg fresh aloe leaf carries out enzymolysis, hydrolysis temperature is 55 DEG C, enzymolysis time is 3 hours, and enzymolysis terminates post-heating to 90 DEG C and carries out enzyme-deactivating in 10 minutes, lowers the temperature 60 DEG C, added for 0.5% decolorizing with activated carbon half an hour, centrifugal filtration, gets supernatant liquid, is Aloe extraction solution.
Prepared by embodiment 5 champignon polypeptide wetting agent
Get Lentinus Edodes extract 30kg, cucumber extract 20kg, collagen polypeptide 20kg, Aloe extraction solution 30kg, above four kinds of solution are fully mixed, obtain pure natural champignon polypeptide wetting agent finished product.
Prepared by embodiment 6 champignon polypeptide wetting agent
Get Lentinus Edodes extract 50kg, cucumber extract 15kg, collagen polypeptide 15kg, Aloe extraction solution 20kg, above four kinds of solution are fully mixed, obtain pure natural champignon polypeptide wetting agent finished product.
Prepared by embodiment 7 champignon polypeptide wetting agent
Get Lentinus Edodes extract 40kg, cucumber extract 20kg, collagen polypeptide 20kg, Aloe extraction solution 20kg, above four kinds of solution are fully mixed, obtain pure natural champignon polypeptide wetting agent finished product.
Prepared by embodiment 8 champignon polypeptide wetting agent
Get Lentinus Edodes extract 40kg, cucumber extract 15kg, collagen polypeptide 15kg, Aloe extraction solution 30kg, above four kinds of solution are fully mixed, obtain pure natural champignon polypeptide wetting agent finished product.
Embodiment 9 champignon polypeptide wetting agent stability test
Sensory testing: the character of perusal product, sees if there is exception;
Heat resistant test: electro-heating standing-temperature cultivator 24h sample being put into (40 ± 1) DEG C, observes whether there is the phenomenon such as variable color, layering, with the heat resistance of judgement sample after recovering room temperature;
Low temperature resistant test: sample is put into (-5 ~-10) DEG C ± electric refrigerator 24h of 1 DEG C, observe whether there is the phenomenon such as variable color, layering, with the cold tolerance of judgement sample after recovering room temperature;
Centrifugal test: sample is placed in centrifuge, with the speed test 30min of (8000) r/min, observes the separation of sample, point layer status.
Experimental result is in table 1:
Table 1 stability test result
Inspection item | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Matched group |
Organoleptic indicator | Without exception | Without exception | Without exception | Without exception | Without exception |
Heat stability | Stable | Stable | Stable | Stable | Stable |
Freeze-stable | Stable | Stable | Stable | Stable | There is Precipitation |
Dewatering ability | Stable | Stable | Stable | Stable | Stable |
Embodiment 10 champignon polypeptide wetting agent performance of keeping humidity is tested
High frequency conductance determining instrument Skicon200 (IBSLtd) is adopted to measure keratodermatitis water content.Experimenter is healthy without dermopathic women, and experiment condition is that the temperature and humidity of test period requirement environment is constant, and temperature is (30 ± 2) DEG C, and relative humidity is 30% ~ 50%.Test zone is skin of forearm, and size is the region of 5cm × 5cm.
Performance of keeping humidity test result is in table 2:
Table 2 performance of keeping humidity test result
As can be seen from performance of keeping humidity result, the long-acting moistening effect of wetting agent prepared by the present invention clearly, compared with matched group, all has significant difference (P < 0.05).
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a compositions, is characterized in that, comprises Lentinus Edodes or Lentinus Edodes extract, Fructus Cucumidis sativi or Fructus Cucumidis sativi extract, collagen protein, Fructus Cucumidis sativi or Fructus Cucumidis sativi extract.
2. compositions according to claim 1, is characterized in that, it is made up of the raw material of following weight portion:
3. compositions according to claim 1 and 2, is characterized in that, the preparation method of described Lentinus Edodes extract comprises the steps:
Step 1: get Lentinus Edodes and pulverize, after mixing with water, heating, cooling;
Step 2: get the product of step 1 after ultrasonic tear reason, adjust ph is 8.5, adds protease, enzymolysis;
Step 3: the enzymatic hydrolysate getting step 2, adjust ph is 5.0, adds protease, enzymolysis, enzyme-deactivating, cooling;
Step 4: the pH value of the enzymatic hydrolysate of regulating step 3 is 7.0, heating, cooling, centrifugal filtration, collects filtrate;
Step 5: add decolorizing with activated carbon in the filtrate that step 4 obtains, filter, collect filtrate; Get described filtrate through ultrafilter membrane ultrafiltration, collect ultrafiltrate, obtain champignon polypeptide extract.
4. the compositions according to any one of claims 1 to 3, is characterized in that, described in the preparation method step 2 of described Lentinus Edodes extract, protease is alkaline protease, trypsin or papain;
In step 2, every kg dried thin mushroom adds the described protease of 5.0 million international units Rate activity;
The temperature of enzymolysis described in step 2 is 50 DEG C, and the time of described enzymolysis is 180min.
5. the compositions according to any one of Claims 1-4, is characterized in that, the preparation method of described Fructus Cucumidis sativi extract comprises the steps:
Step 1: get Fructus Cucumidis sativi and squeeze the juice, collects Sucus Cucumidis sativi; Get Fructus Cucumidis sativi slag to mix with water, heating, cooling, adds plant composite hydrolytic enzyme, enzymolysis, enzyme-deactivating, filters, and collects filtrate;
Step 2: the Sucus Cucumidis sativi of combining step 1 and filtrate, add carrageenan, mixing, leave standstill, collected by centrifugation supernatant, obtains Fructus Cucumidis sativi extract.
6. the compositions according to any one of claim 1 to 5, is characterized in that, in the preparation method step 1 of described Fructus Cucumidis sativi extract, every kg Fructus Cucumidis sativi slag adds the described plant composite hydrolytic enzyme of 3.0 million international units Rate activity;
The temperature of enzymolysis described in step 1 is 50 DEG C, and the time of described enzymolysis is 1h.
7. the compositions according to any one of claim 1 to 6, is characterized in that, the preparation method of described collagen protein comprises the steps:
Step 1: get fish skin or fish scale, mix with water, micronizing, sieve;
Step 2: the product adjust ph of getting step 1 is 8.5, adds alkaline protease, enzymolysis, enzyme-deactivating, cooling;
Step 3: add decolorizing with activated carbon, collecting by filtration filtrate in the enzymatic hydrolysate that step 2 obtains, adjust ph is 6.0, to obtain final product.
8. the compositions according to any one of claim 1 to 7, is characterized in that, the preparation method of described Aloe extract comprises the steps:
Step 1: get Folium Aloe, grinds pulping, adds plant composite hydrolytic enzyme, enzymolysis, enzyme-deactivating, cooling;
Step 2: the enzymatic hydrolysate getting step 1 adds decolorizing with activated carbon, centrifugal filtration, collects filtrate, obtains Aloe extract.
9. the compositions according to any one of claim 1 to 8 is for the preparation of the purposes in the medicine of moisturizing, whitening, health product or cosmetics.
10. a moisturising preparations, is characterized in that, comprises the compositions as described in any one of claim 1 to 8.
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CN105769648A (en) * | 2016-03-01 | 2016-07-20 | 太仓市荣德生物技术研究所 | Water-retention and water replenishment mask liquid |
CN106265239A (en) * | 2016-08-19 | 2017-01-04 | 福建绿宝食品集团有限公司 | A kind of mushrooms mosquito-driving liquid and preparation method thereof |
CN112725399A (en) * | 2020-12-29 | 2021-04-30 | 海南云皓生物科技有限公司 | Preparation method and application of lentinus edodes oligopeptide |
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CN1915351A (en) * | 2006-04-30 | 2007-02-21 | 杭州迪康生物技术有限公司 | Composition of Chinese traditional medicine in use for treating burn and scald, and product for resolving poxes, and preparation method |
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CN104313102A (en) * | 2014-11-06 | 2015-01-28 | 伍曾利 | Method of preparing collagen |
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CN105769648A (en) * | 2016-03-01 | 2016-07-20 | 太仓市荣德生物技术研究所 | Water-retention and water replenishment mask liquid |
CN106265239A (en) * | 2016-08-19 | 2017-01-04 | 福建绿宝食品集团有限公司 | A kind of mushrooms mosquito-driving liquid and preparation method thereof |
CN112725399A (en) * | 2020-12-29 | 2021-04-30 | 海南云皓生物科技有限公司 | Preparation method and application of lentinus edodes oligopeptide |
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