CN103549114B - Application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days - Google Patents
Application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days Download PDFInfo
- Publication number
- CN103549114B CN103549114B CN201310516794.2A CN201310516794A CN103549114B CN 103549114 B CN103549114 B CN 103549114B CN 201310516794 A CN201310516794 A CN 201310516794A CN 103549114 B CN103549114 B CN 103549114B
- Authority
- CN
- China
- Prior art keywords
- wheat gluten
- gluten protein
- protein hydrolysate
- preparation
- hydrolysate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to the application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days, preparation method comprises: taking wheat gluten protein as raw material, add protease hydrolytic, the enzyme that goes out, oxidation processes, concentrated, spraying is dry. The invention has the advantages that: the wheat gluten hydrolysate that (1) adopts the present invention to prepare can significantly improve production performance, the raising intestine of young pigs lactic acid bacteria content of piglet and reduce Escherichia coli quantity; (2) wheat gluten protein hydrolysate low price, addition is unrestricted; (3) technique of the present invention is simply easy to industrialization production, and cost is low.
Description
Technical field
The present invention relates to pig starter feed preparation of batch technology and application thereof, be specifically related to have improve in early days and breakThe preparation method of the wheat gluten protein hydrolysate of milk weaned piglets, reduction diarrhea rate and disconnected in early daysApplication in milk pig starter feed.
Technical background
Large-scale pig farm generally adopts early weaning piglet technology, although this technology has plurality of advantages, earlyWeanling pig not yet grew and improved and the impact of ablactation stress due to self physiological Mechanism phase, generally produced diarrhoeaEarly weaning syndrome with physiological function disorders such as growth retardations. In order to help piglet to obtain effective nutritionSupply need to be added multiple nutrients material and function factor, as probio, egg easy to digest in piglet dietWhite material etc. The raw bacterium of bacterium can play a role by maintaining microecological balance in enteron aisle, therefore comes with probioReplace antibiotic to regulate the balance of gastrointestinal micro-flora, improve breeding performonce fo animals and become animal battalionSupport one of study hotspot. U.S. FDA specifies to allow probio and the Ministry of Agriculture of China of feeding to ratify for 1999The probio kind using comprises: bacillus, streptococcus fecalis, Bifidobacterium, Bacillus acidi lactici, newborn hammerBacterium etc. and brewer's yeast. Protein substance raw material easy to digest comprises whey powder, plasma protein, yeast, fermentation beansThe dregs of rice, fermented fish slurry etc. The protein raw materials not only degradable ANFs that ferments, and can produce little peptide,Lactic acid, vitamin, probio and unknown somatomedin. Invent a kind of feed that improves intestines function of piglingsAdditive and application thereof (application number 201210573776) disclose a kind of raising of intestines function of piglings of improvingFeed additives and application. It is by chitosan copper, chitosan-zn, chitosan ferrous, four kinds of things of chitosan seleniumTwo or more material in matter by a certain percentage compatibility processes, and in pig starter feed without addingAdd two or more the inorganic microelement in this compatibility, in pig starter feed, add 0.05%～2%The feed addictive of raising intestines function of piglings of the present invention, can significantly reduce the diarrhea rate of piglet, improves dayWeightening finish, reduces material anharmonic ratio, strengthens piglet disease-resistant power. Invention weanling pig feed (application number201210545393) disclose a kind of weanling pig feed, this feed specifically consists of by weight percentage:Corn 50.5～63%, dregs of beans 8～13.5%, fermentation soybean 4.5～7.5%, plasma protein 1.5～3%,Fish meal 4～6%, expanded soybean 7.5～13%, whey powder 3～6.5%, inferior powder 0.5～1.5%, vegetable oil0.25～0.85%, premix 1.25～4.15%, wherein premix is made up of additive and pre-mixing agent, addsAgent specifically consists of by weight percentage: trace element 0.35～0.4%, multidimensional 0.03～0.04%, 10%Colistin sulfate 0.05～0.06%, complex enzyme 0.01～0.02%, acidulant 0.01～0.02%, sodium bicarbonate0.05～0.06%, lysine 0.1～0.2%, methionine 0.05～0.2%, choline 0.25～0.3%, premixAgent specifically consists of by weight percentage: stone flour 0.7～1%, calcium monohydrogen phosphate 1.3～1.5%, salt 0.25～0.35%. This invention feed can be strengthened the nutrition of weanling pig, improves the immunity of weanling pig. InventionA kind of additive for feed for piglets (application number 201110247556) discloses a kind of additive for feed for piglets,By hawthorn 20-40 gram, Fructus Hordei Germinatus 20-40 gram, Radix Isatidis 10-20 gram, dried orange peel 10-20 gram, rhizoma atractylodis 10-20Gram, sealwort 10-20 gram, Chinese bulbul 10-20 gram, garlic 5-10 gram, garden burnet 5-10 gram, ginger 5-10Gram, after drying by above proportioning combination, grind into powder and mix, in diet, add in the ratio of diet weight 1%Can. Up to the present there is not yet the report that protein hydrolysate is applied in weanling pig feed.
Summary of the invention
The object of the invention is to overcome prior art above shortcomings, a kind of wheat gluten protein is providedThe preparation method of hydrolysate and application thereof. The wheat gluten hydrolysate that adopts the present invention to prepare can significantly improveProduction performance, raising intestine of young pigs lactic acid bacteria content and the reduction Escherichia coli quantity of piglet.
For achieving the above object, the technical solution used in the present invention is as follows:
A preparation method for wheat gluten protein hydrolysate, taking wheat gluten protein as raw material, by controllingEnzymolysis, the enzyme that goes out, oxidation processes, spraying is dry to be prepared.
In the preparation method of above-mentioned wheat gluten protein hydrolysate, comprise the following steps:
(1) wheat gluten protein is mixed with 5-10 times of weight water, be warming up to 40-60 DEG C, add proteaseIt is 25-35% that stirring is hydrolyzed into degree of hydrolysis, is warming up to 80-90 DEG C and keeps the 30-60min enzyme that goes out, and obtains wheat glutenProtein hydrolyzate;
(2) wheat gluten protein hydrolyzate is cooled to 30-40 DEG C, adds wheat gluten protein hydrolysis liquid-solidThe sugar of shape thing weight 1-3%, carries out oxidation processes with filtrated air;
(3) the wheat gluten protein hydrolyzate after oxidation processes is crossed to colloid mill, concentrated, spraying is dry,Wheat gluten protein hydrolysate.
Further optimize, the described protease of step (2) is in papain, bromelain or pancreatinAny one.
Further optimize, described sugar is any one in glucose, sucrose and fructose.
Further improved, described oxidation processes is that 1L wheat gluten protein enzymolysis liquid passes intoThe filtrated air of 200ml/min-1000ml/min, keeps 1h-6h.
The wheat gluten protein hydrolysate being made by above-mentioned preparation method, with 0.1-50%(wt/wt) add toIn pig starter feed, can significantly improve production performance, the raising intestine of young pigs lactic acid bacteria content of piglet and reduce large intestineBacillus quantity.
The present invention adopts above-mentioned technique, possesses following advantage and remarkable technique effect with respect to prior art:
(1) adopt the wheat gluten hydrolysate prepared of the present invention can significantly improve piglet production performance,Improve intestine of young pigs lactic acid bacteria content and reduce Escherichia coli quantity;
(2) wheat gluten protein hydrolysate low price, addition is unrestricted;
(3) technique of the present invention is simply easy to industrialization production, and cost is low.
Detailed description of the invention
Below in conjunction with implementing, enforcement of the present invention is described further, but enforcement of the present invention and protection modelEnclose and be not limited to this.
Choose 80 of the heavy weanling pigs of 5-6kg, approach by body weight, parity, the principle of sex half and half is divided at randomBecome 4 groups, every group of 20 piglets. Experimental period is 30 days. 4 groups of test piglet basal diet compositions and nutrition are equalIdentical, concrete mass percent is as follows: the content dregs of beans 9.00% of corn 55.00%, 46% protein, send outFerment dregs of beans 5.50%, expanded soybean 4.50%, swelling soya dreg 6%, whey powder 6%, glucose 3%, fish meal 5.00%,Sucrose 2.00%, soybean oil 1.00%, complex vitamin premix 3.00%.
After 2 hours, taking just starting weight and last weight in on-test with end empty stomach in morning on the same day, is list by groupFeed consumption is calculated in position. Concrete formula is as follows:
Weightening finish (kg/ head)=average end heavy (kg/ head)-average initial weight (kg/ head)
Average daily gain (kg/ head)=average weight gain/test number of days
Diarrhea rate (%)=(a diarrhoea number * diarrhoea number of days)/(test pig's head is counted * test number of days) * 100%
Select 6 and butcher at random from every group of piglet, under aseptic condition, gather respectively duodenum,Jejunum, ileum, caecum and colonic contents 5g, for intestinal colony quantitative measurement. Wherein lactic acid bacteria is surveyedSurely adopt MRS agar medium, plate count method is measured. Coil determination adopts Mai KangkaiqiongFat culture medium, plate count method is measured.
Table 1 is the impact of wheat gluten protein hydrolysate on Production Performance of Weaning Pigs
Table 2 is the impact of wheat gluten protein hydrolysate on weanling pig Microflora
Embodiment 1
(1) wheat gluten protein is mixed with 5 times of weight water, be warming up to 40 DEG C, add pancreatin and stir waterSolution to degree of hydrolysis is 25%, is warming up to 80 DEG C and keeps the 60min enzyme that goes out, and obtains wheat gluten protein hydrolyzate;
(2) wheat gluten protein hydrolyzate is cooled to 40 DEG C, adds wheat gluten protein hydrolyzate solidThe glucose of thing weight 1%, carries out oxidation processes with filtrated air; Described oxidation processes is 1L wheat glutenProtein enzymatic hydrolyzate passes into the filtrated air of 200ml/min, keeps 6h.
(3) the wheat gluten protein hydrolyzate after oxidation processes is crossed to colloid mill, concentrated, spraying is dry,No. 1, wheat gluten protein hydrolysate.
No. 1, wheat gluten protein hydrolysate and wheat gluten protein hydrolysate are added respectively to 5% He No. 210%(wt/wt) to piglet basal diet, control group add respectively 5% and 10%(wt/wt) wheyPowder. Specific experiment data are in table 1 and table 2.
From table 1, to add after wheat gluten protein hydrolysate, the daily gain of weanling pig obviously increases,And diarrhea rate is with the increase of wheat gluten protein hydrolysate addition, on a declining curve.
From table 2, add after wheat gluten protein hydrolysate, duodenum in weanling pig, jejunum,In ileum, caecum and colon, lactic acid bacterium number is in rising trend, and Escherichia coli quantity is on a declining curve. ThisAlong with the increase of wheat gluten protein hydrolysate addition, duodenum in weanling pig, jejunum, return outward,In intestines, caecum and colon, lactic acid bacterium number increase trend is more obvious.
Table 1
Table 2
Embodiment 2
(1) wheat gluten protein is mixed with 8 times of weight water, be warming up to 50 DEG C, add bromelainIt is 30% that stirring is hydrolyzed into degree of hydrolysis, is warming up to 85 DEG C and keeps the 40min enzyme that goes out, and obtains wheat gluten protein hydrolysisLiquid;
(2) wheat gluten protein hydrolyzate is cooled to 35 DEG C, adds wheat gluten protein hydrolyzate solidThe sucrose of thing weight 2%, carries out oxidation processes with filtrated air. Described oxidation processes is 1L wheat gluten eggWhite enzymolysis liquid passes into the filtrated air of 500ml/min, keeps 3h.
(3) the wheat gluten protein hydrolyzate after oxidation processes is crossed to colloid mill, concentrated, spraying is dry,No. 2, wheat gluten protein hydrolysate.
Embodiment 3
(1) wheat gluten protein is mixed with 10 times of weight water, be warming up to 60 DEG C, add papainIt is 25-35% that stirring is hydrolyzed into degree of hydrolysis, is warming up to 80-90 DEG C and keeps the 30-60min enzyme that goes out, and obtains wheat glutenProtein hydrolyzate;
(2) wheat gluten protein hydrolyzate is cooled to 30-40 DEG C, adds wheat gluten protein hydrolysis liquid-solidThe fructose of shape thing weight 3%, carries out oxidation processes with filtrated air; Described oxidation processes is 1L wheat glutenProtein enzymatic hydrolyzate passes into the filtrated air of 1000ml/min, keeps 6h.
(3) the wheat gluten protein hydrolyzate after oxidation processes is crossed to colloid mill, concentrated, spraying is dry,Wheat gluten protein hydrolysate, its effect and embodiment 1, embodiment 2 approach.
Claims (3)
1.A kind of early-weaned piglets feed of being used forThe preparation method of wheat gluten protein hydrolysate, is characterized in that taking wheat gluten protein as raw material, by controlled enzymatic hydrolysis, and the enzyme that goes out, oxidation processes, spraying is dry to be prepared, and specifically comprises the following steps:
(1) wheat gluten protein is mixed with the water of 5-10 times of wheat gluten protein weight, be warming up to 40-60 DEG C, add protease and stir that to be hydrolyzed into degree of hydrolysis be 25%-35%, be warming up to 80-90 DEG C and keep the 30-60min enzyme that goes out, obtain wheat gluten protein hydrolyzate;
(2) wheat gluten protein hydrolyzate is cooled to 30-40 DEG C, adds the sugar of wheat gluten protein hydrolyzate solid content weight 1%-3%, carry out oxidation processes with filtrated air; Described protease is bromelain; Described sugar is glucose or fructose; Described oxidation processes is the filtrated air that 1L wheat gluten protein enzymolysis liquid passes into 200ml/min-600ml/min, keeps 1h-6h;
(3) the wheat gluten protein hydrolyzate after oxidation processes is crossed to colloid mill, concentrated, spraying is dry, obtains wheat gluten protein hydrolysate.
2. the wheat gluten protein hydrolysate being made by preparation method described in claim 1, can improve production performance, the raising intestine of young pigs lactic acid bacteria content of piglet and reduce Escherichia coli quantity.
3. the wheat gluten protein hydrolysate application in weanling pig feed in early days described in claim 2, it is characterized in that adding 0.1%-50%(wt/wt in weanling pig feed) described wheat gluten protein hydrolysate, can significantly improve piglet production performance, improve intestine of young pigs lactic acid bacteria content and reduce Escherichia coli quantity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310516794.2A CN103549114B (en) | 2013-10-28 | 2013-10-28 | Application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310516794.2A CN103549114B (en) | 2013-10-28 | 2013-10-28 | Application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103549114A CN103549114A (en) | 2014-02-05 |
| CN103549114B true CN103549114B (en) | 2016-05-25 |
Family
ID=50003619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310516794.2A Expired - Fee Related CN103549114B (en) | 2013-10-28 | 2013-10-28 | Application in wheat gluten protein hydrolysate and preparation method thereof and weanling pig feed in early days |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103549114B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186921B (en) * | 2014-09-23 | 2017-03-08 | 福州大学 | A kind of method of modifying of wheat gluten protein and the application in Yoghourt product |
| CN106978459A (en) * | 2017-04-12 | 2017-07-25 | 山西双林富农林开发有限公司 | A kind of shiny-leaved yellowhorn protein peptides and preparation method thereof, protein peptide health product |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3898345A (en) * | 1971-01-12 | 1975-08-05 | Mars Ltd | Method for preparing meat-like protein food |
| CN1844159A (en) * | 2006-04-07 | 2006-10-11 | 华南理工大学 | Method for graft polymerization of enzymolyzed wheat mucedin and polysaccharide |
| CN101156645A (en) * | 2007-11-06 | 2008-04-09 | 江苏大学 | A wheat gluten proteolysis matter and method for making same as well as its usage |
| CN101331935A (en) * | 2007-06-25 | 2008-12-31 | 北京中融百鸣科技有限责任公司 | Method for preparing meat essence using oxygen heating reaction |
| CN101627793A (en) * | 2009-07-31 | 2010-01-20 | 郑州新威营养技术有限公司 | Industrial production method of hydrolyzed wheat protein for feeding |
| CN101669568A (en) * | 2009-08-24 | 2010-03-17 | 天津春发食品配料有限公司 | Preparation method of modified solubilizing isolated soy protein |
-
2013
- 2013-10-28 CN CN201310516794.2A patent/CN103549114B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3898345A (en) * | 1971-01-12 | 1975-08-05 | Mars Ltd | Method for preparing meat-like protein food |
| CN1844159A (en) * | 2006-04-07 | 2006-10-11 | 华南理工大学 | Method for graft polymerization of enzymolyzed wheat mucedin and polysaccharide |
| CN101331935A (en) * | 2007-06-25 | 2008-12-31 | 北京中融百鸣科技有限责任公司 | Method for preparing meat essence using oxygen heating reaction |
| CN101156645A (en) * | 2007-11-06 | 2008-04-09 | 江苏大学 | A wheat gluten proteolysis matter and method for making same as well as its usage |
| CN101627793A (en) * | 2009-07-31 | 2010-01-20 | 郑州新威营养技术有限公司 | Industrial production method of hydrolyzed wheat protein for feeding |
| CN101669568A (en) * | 2009-08-24 | 2010-03-17 | 天津春发食品配料有限公司 | Preparation method of modified solubilizing isolated soy protein |
Non-Patent Citations (1)
| Title |
|---|
| 谷朊粉酶解物对断奶仔猪生产性能、腹泻及消化率的影响;王石，等;《粮食与饲料工业》;20111231(第2期);第55-57、60页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103549114A (en) | 2014-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102366030B (en) | Mixed composite microbial feed for freshwater fish and production method thereof | |
| CN107927384A (en) | A kind of dustless creep mixed feed for piglets of nonreactive and preparation method thereof | |
| CN105341397A (en) | Fattening pig feed containing Chinese herbal medicines | |
| CN105325719A (en) | Growth promoting fodder special for growing and fattening pigs | |
| CN102178091B (en) | Baking type suckling pig feed before weaning and preparation method thereof | |
| CN103704487A (en) | Compound feed additive for piglet growth promotion and immunity enhancement | |
| CN104509746A (en) | Preparation method of lactating sow feed | |
| CN102125163A (en) | Feed ferment and method for producing fermented feed by utilizing same | |
| CN106417964A (en) | Nutrient-rich green broiler feed and preparation method thereof | |
| CN103652452A (en) | Novel biological feed for prawn and application thereof | |
| CN103798553A (en) | Environment-friendly pig feed additive and preparation method thereof | |
| CN104664106A (en) | Composite probiotic preparation product for piglets, and preparation method of composite probiotic preparation product | |
| CN102499325B (en) | Piglet microbial fermentation antibiotic-free creep feed | |
| CN105104786A (en) | Pigling Chinese herbal medicine probiotics anti-diarrhoea complete formula feed and preparation method therefor | |
| CN105639090A (en) | Preparation method of livestock and poultry acidification and glycolysis feed | |
| CN103315147A (en) | Piglet puffed compound feed and preparation method thereof | |
| CN105076862A (en) | Anti-stress premix capable of promoting growth of piglets and preparation method of anti-stress premix | |
| CN104431560A (en) | Pig feed additive with efficacies of protecting intestinal tracts and improving food calling function | |
| CN104397409A (en) | Biological antimicrobial-free complete feed for medium and big pigs and preparation method of biological antimicrobial-free complete feed | |
| CN108077629A (en) | A kind of nourishing the stomach shield rice noodle roll grain mixed type feed for weaned and preparation method thereof | |
| CN106490364A (en) | A kind of compound feed for piglets and preparation method thereof | |
| CN105053566A (en) | Cornus walteri seed proteinase feed additive and preparation method thereof | |
| CN105815549A (en) | Amino acid high protein powder feed additive and preparation method thereof | |
| CN101497910A (en) | Method for preparing enzymolysis oligopeptide protein | |
| CN101589771A (en) | The preparation method of bio-additive premixed feed special for pigs and bioactive substance carrier thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160525 Termination date: 20201028 |