CN103517919B - 在重组fviii的生产中提高真核细胞生产率的方法 - Google Patents

在重组fviii的生产中提高真核细胞生产率的方法 Download PDF

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CN103517919B
CN103517919B CN201280021390.XA CN201280021390A CN103517919B CN 103517919 B CN103517919 B CN 103517919B CN 201280021390 A CN201280021390 A CN 201280021390A CN 103517919 B CN103517919 B CN 103517919B
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cell
cells
rfviii
suspending liquid
culture
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CN103517919A (zh
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皮特·埃扎娃
伊瑞丽·埃基科维斯特
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Octapharma AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280021390.XA 2011-05-13 2012-05-14 在重组fviii的生产中提高真核细胞生产率的方法 Active CN103517919B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11166071 2011-05-13
EP11166071.8 2011-05-13
US201161489406P 2011-05-24 2011-05-24
US61/489,406 2011-05-24
PCT/EP2012/058899 WO2012156356A1 (en) 2011-05-13 2012-05-14 A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii

Publications (2)

Publication Number Publication Date
CN103517919A CN103517919A (zh) 2014-01-15
CN103517919B true CN103517919B (zh) 2016-11-16

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US (1) US9115381B2 (enExample)
EP (3) EP3626737B1 (enExample)
JP (1) JP6178786B2 (enExample)
KR (1) KR101980164B1 (enExample)
CN (1) CN103517919B (enExample)
AU (1) AU2012257736B2 (enExample)
BR (1) BR112013028006A2 (enExample)
CA (1) CA2835053C (enExample)
DK (1) DK3626737T3 (enExample)
ES (1) ES2970057T3 (enExample)
IL (1) IL229058A (enExample)
RU (1) RU2600886C2 (enExample)
WO (1) WO2012156356A1 (enExample)

Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
US6591609B2 (en) 1997-07-15 2003-07-15 New Power Concepts Llc Regenerator for a Stirling Engine
ES2909630T3 (es) 2014-06-04 2022-05-09 Amgen Inc Métodos de recolección en cultivos de células de mamífero
CN107287265B (zh) * 2016-03-31 2018-09-14 正大天晴药业集团南京顺欣制药有限公司 一种制备重组人凝血因子ⅷ的方法
CN112469821B (zh) * 2018-07-26 2021-12-14 正大天晴药业集团股份有限公司 一种制备重组人凝血因子ⅷ的方法
WO2023242238A1 (en) * 2022-06-15 2023-12-21 UCB Biopharma SRL Cell culture processes
CN120005720B (zh) * 2025-04-21 2025-08-08 深圳市茵冠生物科技有限公司 一种人工智能的细胞培养监测方法及系统

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2006103258A1 (en) * 2005-03-29 2006-10-05 Octapharma Ag Method for improved isolation of recombinantly produced proteins
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法

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US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6358703B1 (en) * 1998-12-10 2002-03-19 Bayer Corporation Expression system for factor VIII
NZ516400A (en) * 1999-07-13 2004-02-27 Biovitrum Ab Stable factor VIII compositions
EP1460131A3 (en) 2000-03-22 2005-06-01 Octagene GmbH Production of recombinant blood clotting factors in human cell lines
CN104593277A (zh) * 2004-03-05 2015-05-06 帝斯曼知识产权资产管理有限公司 用于通过连续灌注和交互切向流来培养细胞的方法
JP2006296423A (ja) * 2005-03-24 2006-11-02 Hitachi Ltd 培養槽の制御装置及び培養装置
JP2008534559A (ja) * 2005-04-01 2008-08-28 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 血液凝固ｆｖｉｉｉ類似体
EP1739179A1 (en) 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
PT2634243T (pt) 2006-07-14 2017-12-18 Patheon Holdings I B V Método para melhorar a cultura de células
WO2009090787A1 (ja) * 2008-01-15 2009-07-23 Toray Industries, Inc. 生理活性ペプチドまたはタンパク質製造方法、および組換え動物細胞
SI2300497T1 (sl) * 2008-06-24 2013-02-28 Octapharma Ag Postopek za äťiĺˇäťenje koagulacijskega faktorja viii
AU2009284113B2 (en) 2008-08-21 2015-05-14 Octapharma Ag Recombinantly produced human factor VIII and IX
DE102008049120A1 (de) 2008-09-26 2010-04-01 Bayer Technology Services Gmbh Verfahren zur Reduzierung von Ablagerungen bei der Kultivierung von Organismen
EP2216395A1 (en) 2009-02-09 2010-08-11 Lonza Biologics plc. Bioreactor for the cultivation of mammalian cells
US8580554B2 (en) 2009-07-31 2013-11-12 Baxter International Inc. Method of producing a polypeptide or virus of interest in a continuous cell culture

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Publication number Priority date Publication date Assignee Title
WO2006103258A1 (en) * 2005-03-29 2006-10-05 Octapharma Ag Method for improved isolation of recombinantly produced proteins
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法

Non-Patent Citations (3)

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Engineering Considerations for Process Development in Mammalian Cell Cultivation;Hu Zhang，et al.;《Current Pharmaceutical Biotechnology》;20100131;第11卷(第1期);第103-112页 *
Microwell Engineering Characterization for Mammalian Cell Culture Process Development;Timothy A. Barrett，et al.;《Biotechnology and Bioengineering》;20100201;第105卷(第2期);摘要，第267页表IV,第269页左栏第1段，第270页表V *
Optimisation of the Factor VIII yield in mammalian cell cultures by reducing the membrane bound fraction;Mille Petersen Kolind，et al.;《Journal of Biotechnology》;20110220;第151卷(第4期);第357-362页 *

Also Published As

Publication number Publication date
CN103517919A (zh) 2014-01-15
US20140051123A1 (en) 2014-02-20
US9115381B2 (en) 2015-08-25
AU2012257736B2 (en) 2015-12-10
RU2600886C2 (ru) 2016-10-27
EP3626737B1 (en) 2023-11-29
KR20140021009A (ko) 2014-02-19
EP4353743A2 (en) 2024-04-17
ES2970057T3 (es) 2024-05-24
IL229058A (en) 2017-03-30
EP4353743A3 (en) 2024-07-17
WO2012156356A1 (en) 2012-11-22
KR101980164B1 (ko) 2019-05-20
JP2014513545A (ja) 2014-06-05
JP6178786B2 (ja) 2017-08-09
IL229058A0 (en) 2013-12-31
BR112013028006A2 (pt) 2016-09-06
EP2707387B1 (en) 2019-12-25
RU2013155382A (ru) 2015-06-20
DK3626737T3 (da) 2024-02-05
EP3626737A1 (en) 2020-03-25
EP2707387A1 (en) 2014-03-19
CA2835053C (en) 2020-07-07
CA2835053A1 (en) 2012-11-22
AU2012257736A1 (en) 2013-10-10

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