CN103503886A

CN103503886A - Compound bactericide containing pyrimorph

Info

Publication number: CN103503886A
Application number: CN201310483994.2A
Authority: CN
Inventors: 赵邦斌
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-10-16
Filing date: 2013-10-16
Publication date: 2014-01-15

Abstract

The invention provides a compound bactericide containing pyrimorph. The effective components of the compound bactericide comprise pyrimorph and tebuconazole; the mass ratio of pyrimorph to tebuconazole is (1:80) to (80:1). Pyrimorph and tebuconazole are compounded for killing the common bacteria of crops such as rice and cucumber; due to the difference of action mechanisms of pyrimorph and tebuconazole, pyrimorph and tebuconazole are mutually compounded to extend the sterilizing range and improve the sterilizing effect, and meanwhile, to improve the yield of the crops greatly and reduce the use level per mu and the use cost.

Description

A kind of composite bactericide containing pyrimorph

Technical field

The present invention relates to composition pesticide technical field, be specifically related to a kind of composite bactericide containing pyrimorph.

Background technology

Pyrimorph (Pyrimorph) molecular formula is C22H25N2C1O2, and chemical name is (E)-3-(2-chloropyridine-4-yl)-3-(4-tert-butyl-phenyl)-acryloyl morpholine.Pyrimorph is one of novel pesticide of China's independent intellectual property right, be a kind of special ovicidal Gammaproteobacteria fungi bactericide, its mechanism of action is by changing the permeability of cell wall, causes protoplasm seepage in cell, suppress mycelium protein matter synthetic simultaneously, thereby suppress mycelial growth.By affecting the formation of cell envelope and suppressing cell respiration, particularly responsive to the formation stages of oomycetes sporangiophore and egg spore, it has unique mode of action to the fungi of the Peronosporaceae of phycomycetes and Phytophthora.Medication before fungal spore forms, can suppress Sporulation completely.

Tebuconazole is a kind of efficient, wide spectrum, interior absorption triazole type sterilization pesticide, is sterol demethylation inhibitor, to the mechanism of action of germ, for suppressing the demethylation of ergosterol on its cell membrane, makes germ cannot form cell membrane, thus kill pathogens.Tebuconazole has protection, treats, roots out three large functions; fungicidal spectrum is wide, the lasting period is long; be mainly used in the seed treatment of Important Economic crop or the efficient germicide of foliage spray; can effectively prevent and treat multiple rust, powdery mildew, net blotch, root rot, the head blight of cereal crop, smut and kind pass zonate spot and Rice shealth blighy etc.

Because the former medicine of pyrimorph is still in the patent phase, so its use cost is higher, limited to a great extent its application at home, particularly in some economically less developed regions, be difficult to promote; And the manufacturer of Tebuconazole is many, low price, but because of its service life for a long time, multiple germ has been produced to certain resistance.In addition, pyrimorph is different from the mechanism of action of Tebuconazole, thus they are mutually compositely had to good synergistic effect within the specific limits, but composite about pyrimorph and Tebuconazole at present there is not yet relevant report at home and abroad.

Summary of the invention

Object of the present invention will solve above-mentioned deficiency exactly, and the composite bactericide containing pyrimorph that a kind of cost is low, bactericidal effect is good is provided.

In order to achieve the above object, a kind of composite bactericide containing pyrimorph of the present invention, the effective ingredient of described composite bactericide is comprised of pyrimorph and Tebuconazole, and the mass ratio of described pyrimorph and Tebuconazole is 1:80-80:1.

Further, the mass ratio of described pyrimorph and Tebuconazole is 1:40-40:1.

Further, the mass ratio of described pyrimorph and Tebuconazole is 1:5-19:1.

Further, described effective ingredient quality accounts for the 10-85% of composite bactericide gross mass.

Further, described composite bactericide is made suspending agent, oil-suspending agent, wetting powder or dry suspending agent.

In composite bactericide of the present invention, can comprise carrier and/or thinner, carrier and/or thinner can be any addible components, as filler, solvent, surfactant etc.Also can add other functional aids such as antifreeze, thickener, stabilizing agent, defoamer, disintegrant if desired.Active ingredient and at least one carrier and/or thinner are mixed with the preparations such as each application target final products, particularly suspending agent, oil-suspending agent, wetting powder or dry suspending agent.

Wherein carrier or filler are white carbon, precipitated calcium carbonate, potter's clay, attapulgite, kaolin, diatomite ,Peng ravine soil, sepiolite, zeolite, river sand, quartz sand, talcum powder, can be used alone, also can two kinds or above mixing use.

Described solvent, cosolvent are toluene, dimethylbenzene, chlorobenzene, solvent naphtha, alpha-methyl-naphthalene, turpentine oil, carrene, chloroform, methyl alcohol, ethanol, isopropyl alcohol, butanols, isoamyl alcohol, cyclohexanone, isophorone, acetophenone, ethyl acetate, butyl acetate, N.N-dimethyl formamide, dimethyl sulfoxide (DMSO), methyl cellosolve, ethyl cellosolve etc., and the use of the mixing between different solvents is regular.

Surfactant can be emulsifier, dispersant, wetting agent or bleeding agent.Surfactant can be nonionic with ionic, can be also two or more compound uses.Described emulsifier is alkylphenol-polyethenoxy (polyoxypropylene) ether, Ben-zylphenol Polyoxyethyl Ether, phenethyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, polyoxyethylene carboxylate, phenethyl phenol polyethenoxy polyethenoxy ether, castor oil ethylene oxide adduct, alkylphenol polyoxyethylene formaldehyde condensation products, and the phosphate of these compounds, alkyl benzene sulphonate (sodium salt or calcium salt) or directly use commercially available emulsifier monomer and composite product.Concrete example as agriculture breast 500# series, 600# is serial, 1600# is serial, NP is serial, OP is serial, AEO is serial, 700#, 400# series and composite kind 2201,0203B, 2021B, 4103 etc., these are all emulsifier productions well known in the art, and market is on sale.

Composite bactericide of the present invention is mainly used in preventing and treating rice green smut, rice sheath blight disease, rice blast; Cucumber, downy mildew of garpe; Capsicum, tomato blight; Sigatoka; Apple tree scab; Mandarin tree shot hole etc.Application process mainly adopts and adds water spray and make cauline leaf and process, and its amount of application changes with weather condition and crop state, can reach by the formulation with suitable and prevent and treat object.

The present invention is pyrimorph and the composite use of Tebuconazole, and because the two sterilization mechanism has difference, both are mutually mixed and can produce conflict effect, have expanded on the contrary bactericidal range, and have suitably reduced mu consumption and use cost.

Embodiment

The present invention is elaborated with following specific embodiment, but the present invention only limits to absolutely not these examples.In all formulas, percentage is all weight percentage.The processing technology of the various preparations of the present composition and equipment are known.

Formulation Example 1(suspending agent): pyrimorph 5%, Tebuconazole 25%;

Formulation Example 2(wetting powder): pyrimorph 20%, Tebuconazole 55%;

Formulation Example 3(oil-suspending agent): pyrimorph 15%, Tebuconazole 15%;

Formulation Example 4(suspending agent): pyrimorph 17.5%, Tebuconazole 12.5%;

Formulation Example 5(dry suspending agent): pyrimorph 45%, Tebuconazole 5%;

Formulation Example 6(wetting powder): pyrimorph 38%, Tebuconazole 2%;

Using above-mentioned Formulation Example 1-5 as test medicine, verify that the multiple acid of pyrimorph and Tebuconazole measures the toxicity of cucumber downy mildew germ, for field control effectiveness test application and the practical application of this medicament provides scientific basis, its method and effect are as follows:

Will above each medicament dilution be mixed with 1000,500,200,100, a 50mg/kg5 series concentration, now with the current.

Bacterial classification expands to be cultivated: cut-off footpath is the sterilizing culture dish of 9 cm, each pours respectively 20mlPDA medium into, after condensation, get each fritter of the fresh-keeping bacterial classification of cucumber downy mildew germ and put into respectively in the middle of culture dish, be placed under 28 ℃ of constant temperatures and cultivate, standby when mycelia is covered with culture dish edge.

Experimental scheme is processed: the liquid preparing is put in sterilizing culture dish with sterilizing pipette, extract 1 ml, then with the medium that 10ml pipette, extract 9ml dissolves, puts into ware and mix immediately, every concentration arranges 3 repetitions, take and does not add liquid as contrast.The stainless steel card punch that is 5cm with diameter is beaten and is got the cultured examination germ that supplies, and uses oese picking, puts into cooling mixed medicine medium center, is placed at 28 ℃ and cultivates a couple of days.

Result observation and appraisal: grow to soon Min edge and be as the criterion to contrast mycelia, periodic measurement colony diameter, calculates each concentration colony diameter mean value, represents with straight length, obtains inhibition percentage.Then with concentration logarithm---inhibition percentage probit value is obtained regression beeline equation.According to equation, obtain EC50 value, relatively virulence is big or small, and by each mixture co-toxicity coefficient of EC50 pH-value determination pH.According to toxicity index, theoretical toxicity index, actual toxicity index, 4 indexs of co-toxicity coefficient, its observed result is carried out to comprehensive assay.

The content (%) of toxicity index * B of content (the %)+B of toxicity index * A of theoretical toxicity index=A in mixture in mixture

Take Tebuconazole as standard medicament, and result of the test sees the following form shown in 1:

Table 1: pyrimorph, Tebuconazole and the inhibition percentage of different proportioning to cucumber downy mildew germ thereof

As can be seen from Table 1, each mixture preparation of pyrimorph Tebuconazole all has good inhibitory action to cucumber downy mildew, 1000mg/kg concentration to the inhibition percentage of cucumber downy mildew germ all more than 85%.

Take Tebuconazole as standard medicament, record the co-toxicity coefficient of each mixture in Table 2:

Table 2: pyrimorph, Tebuconazole and different proportioning thereof the toxicity test result to cucumber downy mildew germ

From table 2, Toxicity Determination result shows: pyrimorph and Tebuconazole are composite has greater activity to cucumber downy mildew, the co-toxicity coefficient of each mixture preparation is all greater than 120, have obvious synergistic effect, the co-toxicity coefficient that wherein pyrimorph and Tebuconazole are mixed with 15:15 is up to 172.60.

field control effectiveness test example one,

The conventional late rice disease-preventing and yield-increasing of 30% fourth pyrrole penta oil-suspending agent (Formulation Example 4:17.5:12.5) single cropping test data is in Table 3,30% fourth pyrrole penta oil-suspending agent of the present invention reaches 93.5% to the protection effect of late rice germ, and the growth of paddy rice is brought to remarkable effect, compare with blank, its rate of growth reaches 6%.It is all apparently higher than the protection effect and the rate of growth that contrast medicament jinggangmeisu, benzene first propiconazole.

Table 3: conventional late rice disease-preventing and yield-increasing application form

test of pesticide effectiveness example two:

30% fourth pyrrole penta oil-suspending agent (Formulation Example 3:15:15) single cropping Late-cropping Indica Hybrid Rice disease-preventing and yield-increasing test data is in Table 4, penta 40 grams/acre of 30% fourth pyrroles are 87.53%, to the preventive effect of false smut, are 65.08% the preventive effect of banded sclerotial blight, and rice per mu yield rate of growth is reached to 28.19%.It is apparently higher than preventive effect and the rate of growth of 20 milliliters of 20 milliliters+love of contrast medicament thifluzamide seedlings.

Table 4: to banded sclerotial blight, false smut preventive effect and output application form

test of pesticide effectiveness example three,

The different dispenser of 50% fourth pyrrole penta dry suspending agent (Formulation Example 5:45:5) prevents false smut test in Table 5 period, and general drug effect highlights about ten days, reaches the highest control efficiency.

Table 5: different times medication is to the comparison of single cropping late hybrid rice false smut control efficiency

test of pesticide effectiveness example four,

30% fourth pyrrole penta oil-suspending agent is to the integrated control effect of Main Rice Diseases banded sclerotial blight, false smut and Effects of Increasing Yield:

Experimental field is located in Da Bie Mountain area, is rice green smut, banded sclerotial blight Chang Faqu.Experimental field sowing on May 1st, 2011, May 25 transplanted, and cultivation management is consistent with the local level of production.

Experimental pharmacy: 30% fourth pyrrole penta oil-suspending agent, preparation consumption is respectively 40g/ mu, 50g/ mu, 60g/ mu;

Contrast medicament: 30% benzene first propiconazole, consumption is 20g/ mu;

5% jinggangmeisu, consumption is 100g/ mu;

Administration time: on August 5th, 2011, August 16, paddy rice cut phase+full heading time;

Application method: add water 45KG, foliar spray; Repeat 4 times.

Table 6:30% fourth pyrrole penta oil-suspending agent control rice green smut, banded sclerotial blight experimental result

As can be seen from Table 6,30% fourth pyrrole penta oil-suspending agent of the present invention is up to 88.75% to the preventive effect of Main Rice Diseases banded sclerotial blight, the highest preventive effect to false smut reaches 89.93%, all apparently higher than the integrated control effect that contrasts medicament 30% benzene first propiconazole and 5% jinggangmeisu.It is up to 11.38% to the effect of increasing production of paddy rice in Table the rate of growth of the 60g/ mu of 7,30% fourth pyrrole penta OF.

Table 7:30% fourth pyrrole penta oil-suspending agent increasing production of rice effect experiment

Above described in be only that the preferred embodiment of the present invention is described; not scope of the present invention is limited; design under spiritual prerequisite not departing from the present invention; various distortion and improvement that the common engineers and technicians in this area make technical solution of the present invention, all should fall in the definite protection domain of claims of the present invention.

Claims

1. containing a composite bactericide for pyrimorph, it is characterized in that: the effective ingredient of described composite bactericide is comprised of pyrimorph and Tebuconazole, the mass ratio of described pyrimorph and Tebuconazole is 1:80-80:1.

2. the composite bactericide containing pyrimorph according to claim 1, is characterized in that: the mass ratio of described pyrimorph and Tebuconazole is 1:40-40:1.

3. the composite bactericide containing pyrimorph according to claim 1, is characterized in that: the mass ratio of described pyrimorph and Tebuconazole is 1:5-19:1.

4. the composite bactericide containing pyrimorph according to claim 1, is characterized in that: described effective ingredient quality accounts for the 10-85% of composite bactericide gross mass.

5. according to the composite bactericide containing pyrimorph described in claim 1-4 any one, it is characterized in that: described composite bactericide is made suspending agent, oil-suspending agent, wetting powder or dry suspending agent.