CN103497984A - Preparation method of rice enzymolysis selenium polypeptides with immune activity - Google Patents
Preparation method of rice enzymolysis selenium polypeptides with immune activity Download PDFInfo
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- CN103497984A CN103497984A CN201310386081.9A CN201310386081A CN103497984A CN 103497984 A CN103497984 A CN 103497984A CN 201310386081 A CN201310386081 A CN 201310386081A CN 103497984 A CN103497984 A CN 103497984A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 63
- 235000009566 rice Nutrition 0.000 title claims abstract description 63
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 57
- 239000011669 selenium Substances 0.000 title claims abstract description 57
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 30
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 26
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000005965 immune activity Effects 0.000 title abstract 2
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 62
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 23
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 238000005238 degreasing Methods 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 12
- 238000001556 precipitation Methods 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 12
- 239000003513 alkali Substances 0.000 claims description 11
- 238000003760 magnetic stirring Methods 0.000 claims description 11
- 238000012856 packing Methods 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000003531 protein hydrolysate Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 12
- 210000002540 macrophage Anatomy 0.000 abstract description 8
- 239000006227 byproduct Substances 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 2
- 230000036737 immune function Effects 0.000 abstract 2
- 206010057249 Phagocytosis Diseases 0.000 abstract 1
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 235000015872 dietary supplement Nutrition 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 abstract 1
- 238000000227 grinding Methods 0.000 abstract 1
- 235000013402 health food Nutrition 0.000 abstract 1
- 230000008782 phagocytosis Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 239000011573 trace mineral Substances 0.000 abstract 1
- 235000013619 trace mineral Nutrition 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 229940091258 selenium supplement Drugs 0.000 description 43
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 4
- 230000000242 pagocytic effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 229960002718 selenomethionine Drugs 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010006464 Hemolysin Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101710157860 Oxydoreductase Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 206010039921 Selenium deficiency Diseases 0.000 description 1
- 102000008114 Selenoproteins Human genes 0.000 description 1
- 108010074686 Selenoproteins Proteins 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021112 essential micronutrients Nutrition 0.000 description 1
- 239000003228 hemolysin Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000005462 in vivo assay Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- MYVIATVLJGTBFV-UHFFFAOYSA-M thiamine(1+) chloride Chemical compound [Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N MYVIATVLJGTBFV-UHFFFAOYSA-M 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Cereal-Derived Products (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the processing field of a selenium-rich rice by-product, in particular to a preparation method of rice enzymolysis selenium polypeptides with immune activity. According to the method, selenium-rich rice processing by-product broken rice is used to prepare degreased selenium-rich rice powder by grinding, sieving and degreasing, a supernatant is collected after alkaline extraction and centrifugation by using a sodium hydroxide solution, a dilute hydrochloric acid solution is used for acid precipitation, and after centrifugation, a precipitate is taken for washing and then is frozen-dried. The obtained rice proteins, after enzymolysis by using trypsase, are frozen-dried to obtain the rice enzymolysis polypeptides with the selenium content of 0.3-0.4mg/ kg. In a cell immune function experiment, the rice enzymolysis selenium polypeptides have good capacity for promotion of cell proliferation and neutral red phagocytosis of macrophage. The rice enzymolysis selenium polypeptides are natural in nutrition, rich in trace element selenium, and suitable to use as auxiliary food materials, nutrient supplements or health food for enhancing immune functions of bodies, and the preparation method is simple and reliable.
Description
One, technical field
The present invention relates to a kind of selenium-enriched rice byproduct manufacture field, particularly a kind of preparation method with immunocompetent rice enzymolysis selenium polypeptide, belong to the functional foodstuff manufacture field.
Two, background technology
Selenium is the essential micronutrient element of a kind of human and animal, has become the focus of domestic and international concern.In recent years, some scholars find that selenium has certain restraining effect to tumor growth, and the generation of selenium deficiency and some disease is closely related.Research shows, selenium is also some active centre that participates in the selenoenzyme of cell antioxygenation, as Selenoperoxidase, oxydo-reductase etc.Selenium in rice exists mainly with the combining form of albumen selenium greatly, has the scholar to pass through the morphological analysis research of selenium, finds that in selenium-rich rice, selenium mainly exists with selenomethionine (SeMet) form.As selenium, the main existence form in the life system has very high biological activity to SeMet, and SeMet has the bioavailability higher than one times of Sodium Selenite.
Oneself is acknowledged as the method for comparatively applicable extraction rice protein the methods such as prepared by alkaline process extraction and enzyme process, and wherein, it is modal a kind of in protein extracting method that alkaline process extracts.The rice protein that surpasses 80% can be extracted by this method, its principle is mainly with dilute alkaline soln, water-fast rice protein is dissolved and makes it at isoelectric precipitation with acid solution subsequently.It is to be conceived to destroy hydrogen bond, amido linkage, hydrophobic interaction etc. in large oryzenin that alkaline process extracts, and makes protein molecular weight reduce and improves protein extracting ratio.Recently have and report that this extracting method has improved the degree of digested of rice protein in vitro and in vivo, this is relevant with the modification that protein agent structure, amino acid are formed.
All will be significantly higher than the not rice protein of enzymolysis no matter the amino-acid residue that rice protein generates after enzymolysis or small-molecular peptides are activity in vivo or external activity, a lot of research all utilizes external enzymolysis to obtain to have more active albumen.External enzymolysis has mild condition, does not destroy the advantages such as nutritive ingredient, and specific proteases can produce multiple have anti-oxidant activity or immunocompetent peptide to the Degradation of peptide chain in rice protein.The data that the rice protein biological activity is relevant is also few, but existing analysis and research are enough to confirmation, and the rice protein zymolyte has the characteristics such as anti-oxidant, antiviral and adjusting immunization.Cereal peptide immunocompetence measuring method also can be divided into two kinds of in vivoassay and external tests, external test method has: splenocyte multiplication capacity mensuration, macrophage phagocytic ability mensuration etc., the immunocompetence measuring method comprises in antibody-producting cell assay, serum hemolysin HC50 pH-value determination pH etc. in body, the impact of cereal peptide on the human immune system that had the scholar even to study.
Rich selenium is cracked rice as the selenium-rich rice by product, fails to be fully used, and causes the wasting of resources, therefore, extracts rich selenium and cracks rice and have containing seleno-protein, preparation the comprehensive utilization that immunocompetent selenium polypeptide is more conducive to selenium-rich rice.
Three, summary of the invention
Technical problem
The purpose of this invention is to provide a kind of preparation method with immunocompetent rice enzymolysis selenium polypeptide, effectively utilized selenium-enriched rice processing byproduct---rich selenium is cracked rice, can be used for food auxiliary material or nutrition-fortifying agent.
Technical scheme
Preparation of the present invention is a kind of preparation method with immunocompetent rice enzymolysis polypeptide realize by following steps:
(1) raw materials pretreatment: get fresh rich selenium and crack rice, grind, cross 100 mesh sieves;
(2) degreasing: use normal hexane, solid-liquid ratio is 1: 3～1: 6, in the upper degreasing twice of magnetic stirring apparatus (79-1, Changzhou Guohua Electric Appliance Co., Ltd.), and each 4h, centrifugal, precipitation is placed on to an air-dry night in stink cupboard;
(3) alkali is carried: the NaOH solution that is 0.05mol/L～0.07mol/L by concentration, and solid-liquid ratio 1: 10～1: 20,35～45 ℃ of temperature, time 2h～4h, alkali is carried 2 times, centrifugal, merges supernatant liquor;
(4) acid is heavy: the gained protein hydrolyzate is placed on magnetic stirring apparatus, is adjusted to iso-electric point (4.5～5.0) with dilute hydrochloric acid solution while stirring, treat Precipitation, more centrifugal, wash 2 times;
(5) freeze-drying: above-mentioned gained protein is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put freeze drier freeze-drying (FD-1C, Spain Telstar company) 24 hours, the valve bag of packing into after taking-up into.
(6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 8～1: 10, concentration of substrate 50～70g/L, pH8.5～9.0, enzymolysis 2h～3h under the condition that temperature is 40～50 ℃, centrifugal collection supernatant liquor;
(7) freeze-drying: above-mentioned gained enzymolysis solution is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
Beneficial effect
(1) the present invention has effectively utilized the selenium-enriched rice processed side product---and rich selenium is cracked rice, and not only avoids waste, and has prepared and have immunocompetent rice selenium polypeptide.
(2) to prepare gained rice selenium polypeptide selenium content higher than the content of general rice protein zymolyte in the present invention, can be used as nutrition accessory or nutrition-fortifying agent, has higher utility value.Table 1 is general rice, rich selenium crack rice raw material, rice selenium polypeptide selenium content.
The protein of table 1 rice and Peptides and selenium content
(3) the present invention prepares gained rice selenium polypeptide and has the immunocompetence higher than the general rice protein zymolyte, has higher utility value.In concentration 10-100 μ g/mL scope, the macrophage proliferation rate of rice selenium polypeptide and macrophage phagocytic rate all are significantly higher than general rice protein zymolyte (P<0.05).When 100 μ g/mL, the macrophage proliferation rate of rice selenium polypeptide and macrophage phagocytic rate reach respectively 60% and 76% (seeing Fig. 1 and Fig. 2).
Four, accompanying drawing explanation
To be gained selenium-rich rice selenium polypeptide of the present invention contrast figure with the general rice Peptides to the impact of macrophage proliferation rate to Fig. 1;
To be gained selenium-rich rice selenium polypeptide of the present invention contrast figure with the general rice Peptides to the impact of macrophage phagocytic toluylene red ability to Fig. 2.
The embodiment of invention
Embodiment 1
(1) raw materials pretreatment: same batch of rich selenium is cracked rice and ground with pulverizer, cross afterwards 100 mesh sieves.
(2) degreasing: use normal hexane, solid-liquid ratio is 1: 5, and on magnetic stirring apparatus, degreasing twice, and each 3h is centrifugal, and precipitation is placed on to an air-dry night in stink cupboard.
(3) alkali is carried: by degreasing selenium-rich rice powder, by NaOH concentration, be 0.07mol/L, and solid-liquid ratio 1: 15,40 ℃ of temperature, time 3h, alkali is carried 2 times, centrifugal, merges supernatant liquor.
(4) acid is heavy: the gained protein hydrolyzate is placed on magnetic stirring apparatus, is adjusted to iso-electric point (4.5) with dilute hydrochloric acid solution while stirring, treat Precipitation, more centrifugal, wash 2 times.
(5) freeze-drying: above-mentioned gained protein is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
(6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 8, concentration of substrate 50g/L, pH9.0, enzymolysis 3h under the condition that temperature is 40 ℃, centrifugal collection supernatant liquor;
(7) freeze-drying: above-mentioned gained enzymolysis solution is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
Embodiment 2
(1) raw materials pretreatment: same batch of rich selenium is cracked rice and ground with pulverizer, cross afterwards 100 mesh sieves.
(2) degreasing: use normal hexane, solid-liquid ratio is 1: 6, and on magnetic stirring apparatus, degreasing twice, and each 4h is centrifugal, and precipitation is placed on to an air-dry night in stink cupboard.
(3) alkali is carried: by degreasing selenium-rich rice powder, by NaOH concentration, be 0.07mol/L, and solid-liquid ratio 1: 10,40 ℃ of temperature, time 4h, alkali is carried 2 times, centrifugal, merges supernatant liquor.
(4) acid is heavy: the gained protein hydrolyzate is placed on magnetic stirring apparatus, is adjusted to iso-electric point (4.5) with dilute hydrochloric acid solution while stirring, treat Precipitation, more centrifugal, wash 2 times.
(5) freeze-drying: above-mentioned gained protein is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
(6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 9, concentration of substrate 60g/L, pH9.0, enzymolysis 3h under the condition of temperature 45 C, centrifugal collection supernatant liquor;
(7) freeze-drying: above-mentioned gained enzymolysis solution is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
Embodiment 3
(1) raw materials pretreatment: same batch of rich selenium is cracked rice and ground with pulverizer, cross afterwards 100 mesh sieves.
(2) degreasing: use normal hexane, solid-liquid ratio is 1: 5, and on magnetic stirring apparatus, degreasing twice, and each 3h is centrifugal, and precipitation is placed on to an air-dry night in stink cupboard.
(3) alkali is carried: by degreasing selenium-rich rice powder, by NaOH concentration, be 0.07mol/L, and solid-liquid ratio 1: 20,40 ℃ of temperature, time 4h, alkali is carried 2 times, centrifugal, merges supernatant liquor.
(4) acid is heavy: the gained protein hydrolyzate is placed on magnetic stirring apparatus, is adjusted to iso-electric point (4.5) with dilute hydrochloric acid solution while stirring, treat Precipitation, more centrifugal, wash 2 times.
(5) freeze-drying: above-mentioned gained protein is installed and puts into refrigerator with culture dish, and (at one night of ℃ lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up-20.
(6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 10, concentration of substrate 60g/L, pH8.5, enzymolysis 3h under the condition of temperature 50 C, centrifugal collection supernatant liquor;
(7) freeze-drying: above-mentioned gained enzymolysis solution is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
Claims (4)
1. the preparation method with immunocompetent rice enzymolysis polypeptide is characterized in that comprising the steps and processing condition:
(1) raw materials pretreatment: get fresh rich selenium and crack rice, grind, cross 100 mesh sieves;
(2) degreasing: use normal hexane, solid-liquid ratio is 1: 3～1: 6, in the upper degreasing twice of magnetic stirring apparatus (79-1, Changzhou Guohua Electric Appliance Co., Ltd.), and each 4h, centrifugal, precipitation is placed on to an air-dry night in stink cupboard;
(3) alkali is carried: the NaOH solution that is 0.05mol/L～0.07mol/L by concentration, and solid-liquid ratio 1: 10～1: 20,35～45 ℃ of temperature, time 2h～3h, alkali is carried 2 times, centrifugal, merges supernatant liquor;
(4) acid is heavy: the gained protein hydrolyzate is placed on magnetic stirring apparatus, is adjusted to iso-electric point (4.5～5.0) with dilute hydrochloric acid solution while stirring, treat Precipitation, more centrifugal, wash 2 times;
(5) freeze-drying: above-mentioned gained protein is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put freeze drier freeze-drying (FD-1C, Spain Telstar company) 24 hours, the valve bag of packing into after taking-up into;
(6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 8～1: 10, concentration of substrate 50～70g/L, pH8.5～9.0, enzymolysis 2h～3h under the condition that temperature is 40～50 ℃, centrifugal collection supernatant liquor;
(7) freeze-drying: above-mentioned gained enzymolysis solution is installed and puts into refrigerator with culture dish, and-20 ℃ of one nights of lower pre-freeze, second day is put the freeze drier freeze-drying into 24 hours, the valve bag of packing into after taking-up.
2. rice selenium polypeptide preparation method according to claim 1, it is characterized in that (2) degreasing: use normal hexane, solid-liquid ratio is 1: 3～1: 6, and on magnetic stirring apparatus, degreasing twice, each 4h is centrifugal, and precipitation is placed on to an air-dry night in stink cupboard.
3. rice selenium polypeptide preparation method according to claim 1 is characterized in that (3) alkali carries: by degreasing selenium-rich rice powder, by NaOH concentration, be 0.05～0.07mol/L, and solid-liquid ratio 1: 10～1: 20,35～45 ℃ of temperature, time 2～4h.
4. rice selenium polypeptide preparation method according to claim 1, it is characterized in that (6) enzymolysis: by above-mentioned gained albumen dry powder at trypsinase with concentration of substrate than 1: 8～1: 10, concentration of substrate 50～70g/L, pH8.5～9.0, enzymolysis 2h～3h under the condition that temperature is 40～50 ℃.
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| CN108651995A (en) * | 2017-04-01 | 2018-10-16 | 新疆喀什昆仑翠翎鸽业有限责任公司 | One kind is rich in active peptides dove Blood piece agent and preparation method thereof of enriching blood |
| CN108753721A (en) * | 2018-06-23 | 2018-11-06 | 淮安诺康生物科技有限公司 | The Activiation method of LAK LAK |
| CN114287506A (en) * | 2021-11-05 | 2022-04-08 | 安徽中志科技有限公司 | Preparation method of easy-to-absorb edible fungus protein powder with high organic selenium content |
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| CN104480165A (en) * | 2014-11-24 | 2015-04-01 | 南京农业大学 | Method for producing selenium-enriched maltose by treating selenium-enriched rice in enzymatic method |
| CN104472851A (en) * | 2014-11-24 | 2015-04-01 | 南京农业大学 | Method for producing selenium-enriched protein powder by treating selenium-enriched rice in enzymatic method |
| CN104472851B (en) * | 2014-11-24 | 2017-10-10 | 南京农业大学 | It is a kind of to be enzymatically treated the method that selenium-rich rice produces selenium rich of egg white powder |
| CN108651995A (en) * | 2017-04-01 | 2018-10-16 | 新疆喀什昆仑翠翎鸽业有限责任公司 | One kind is rich in active peptides dove Blood piece agent and preparation method thereof of enriching blood |
| CN108753721A (en) * | 2018-06-23 | 2018-11-06 | 淮安诺康生物科技有限公司 | The Activiation method of LAK LAK |
| CN114287506A (en) * | 2021-11-05 | 2022-04-08 | 安徽中志科技有限公司 | Preparation method of easy-to-absorb edible fungus protein powder with high organic selenium content |
| CN115989779A (en) * | 2023-03-13 | 2023-04-21 | 辽宁大学 | Organic quantitative selenium-rich cultivation method for northeast single garlic |
| CN116370605A (en) * | 2023-05-15 | 2023-07-04 | 山东博森医学工程技术有限公司 | Medicine for improving proliferation and phagocytic capacity of immune cells |
| CN116370605B (en) * | 2023-05-15 | 2023-09-12 | 山东博森医学工程技术有限公司 | Medicine for improving proliferation and phagocytic capacity of immune cells |
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