CN103467771B - A kind of preparation method and its usage of marine bioactivity bleeding-stopping dressing - Google Patents
A kind of preparation method and its usage of marine bioactivity bleeding-stopping dressing Download PDFInfo
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- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of preparation method and its usage of marine bioactivity bleeding-stopping dressing, that the acetum of the aqueous solution containing HACC and/or chitosan is added the sodium alginate soln of equivalent as 1 liquid when stirring, using the emulsion containing paraffin as 2 liquid, 1 liquid and 2 liquid are become stable emulsification system according to the ratio mixing and stirring of 2 ~ 3:5, add linking agent calcium chloride solution and make its crosslinking curing, then be drying to obtain by washing dehydration.The material that the microspheres product that the present invention makes adopts has good biocompatibility and biodegradability, and antibacterial, hemostasis, the short specific function such as more, the product of crosslinking curing is when contacting with the surface of a wound, moisture in the quick draw blood of energy, Platelet Concentrate and thrombin become viscogel shape, thus the hemorrhage surface of a wound of effective sealing, enhance the anthemorrhagic performance of micro-sphere material, because of but a kind of stable, efficiently, the biological activity bleeding-stopping dressing of environmental protection.
Description
Art
The present invention relates to a kind of bleeding-stopping dressing, particularly relate to a kind of preparation method and its usage of marine bioactivity bleeding-stopping dressing.
Background technology
Chitosan is that content is only second to cellulosic second largest native biopolymer material, there is nontoxic, good biocompatibility, anti-inflammatory, anti, antibacterial, wound healing and moisture-absorbing moisture-keeping and easy many excellent properties such as degraded, can be degraded by the enzyme lysozyme as the available monose of body, show good biodegradability, and play Blood clotting by number of ways.Quaternary Ammonium Salt of Chitosan is a kind of cats product, positively charged in water, can adsorb and antimicrobial surface, form micelle, and progressively infiltrate cytoplasmic class lipid layer and protein, thus change membrane passage, be that entocyte leaks, cause microbial death; Also can make the structure generation sex change of albumen and enzyme, the metabolism of destroy microorganisms, kills microorganism simultaneously.Lalgine is the natural polyanions polysaccharide extracted in brown alga, itself and chitosan have similar performance, after sodium alginate and calcium chloride are cross-linked, alginate calcium can be generated, after the alginate calcium generated contacts with the purulence blood on wound, meeting and the sodium ion generation ion-exchange in body fluid, be converted into the gel of stickiness while discharging calcium ion.Large quantity research shows, after chitosan and alginate calcium are interacted by certain physics or chemical mode, can be formed urgees to heal acts on more by force, antibacterial effect is better, haemostatic effect is better.The composite marine biomaterial that mechanical property is more excellent.
Wound hemorrhage is one of important illness of harm humans health and lives safety, no matter War And Peace period, and unmanageable profuse bleeding causes wounded's main causes of death.The war wound caused owing to losing blood in war is cut personnel larger proportion, and the person killed in action of about 50% is before delivering to dressing station according to statistics, dead in several minutes because of severe loss of blood.Injured hemorrhage also very common in peacetime various unexpected incidents.China's traffic accident and the casualties are continuous ascendant trend is exactly typical case.Simply, fast and effectively Hemostasis to minimizing disability and injures and deaths significant.Traditional dressing is as the dressing of all kinds of gauze type, and major function prevents wound infection, the protection surface of a wound, but to promoting that the effect of wound healing is little, even also can affect wound healing, and the effect surface of a wound that can not be fully effective.Therefore novel hemostatic material and apparatus are wound hemostasis urgent problems, are subject to the great attention of countries in the world medical science and military medicine.Along with going deep into researching wound healing, use the object of dressing far from order to flap coverage, hemostatic and antibacterial and Promotive union have become the certainty of hemostatic material development.Existing hemostatic material is confined to simple hemostasis more, but to anti-infective, promoting healing, promotion tissue repair, that prevention is adhered, reduces the research of the aspects such as scar is also fewer.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation method of marine bioactivity bleeding-stopping dressing, and this bleeding-stopping dressing has efficient anthemorrhagic performance to the surface of a wound, and have antibacterial, promote tissue repair, prevention be adhered, biodegradability.
For solving the problems of the technologies described above, the preparation method of this marine bioactivity bleeding-stopping dressing is:
The acetum of the aqueous solution containing HACC and/or chitosan is added the sodium alginate soln of equivalent as 1 liquid when stirring, using the emulsion containing paraffin as 2 liquid, 1 liquid and 2 liquid are become stable emulsification system according to the ratio mixing and stirring of 2 ~ 3:5, add linking agent calcium chloride solution and make its crosslinking curing, then be drying to obtain by washing dehydration.
Described HACC and chitosan solution have following general formula:
or
Wherein, polymerization degree n is between 300 ~ 2000, and relative molecular mass is 8.4 × 10
4~ 5.6 × 10
5between;
The general structure of described Lalgine sodium salt is as follows:
Wherein, polymerization degree n is between 180-930, and relative molecular mass is 3.2 × 10
4~ 2.5 × 10
5between.
Described HACC and the compound method of chitosan-acetic acid solution are: be dissolved in distilled water by the HACC of equivalent, be mixed with the aqueous solution that mass percentage concentration is 1.6 ~ 2.4%, chitosan being joined volume ratio is in 1 ~ 2:100 acetum, stirs and is mixed with the colloidal solution that mass percentage concentration is 1.6 ~ 2.4%.
The mass percentage concentration of described sodium alginate soln is 1 ~ 2%, is to enter sodium alginate in distilled water during preparation, is mixed with micro-yellow milky solution.
The compound method of the emulsified soln of described paraffin is: class 80 of department, tween 80 and stearic acid are mixed into emulsifying agent according to the mass ratio of 9:9:1 ~ 3, and emulsifying agent and whiteruss are mixed according to the mass ratio of 12 ~ 13:125, at 72 ~ 78 DEG C, stir into O(water)/W(oil) emulsion.
The massfraction of linking agent calcium chloride is 1.6% ~ 3.2%, and it joins in emulsification system liquid according to the volume ratio with emulsification system 1:12 ~ 18, and the temperature of reaction is 70 ~ 80 DEG C, and the reaction times is 50 ~ 60min.
Described washing is washings washing, and described washings is Virahol, and except de-emulsifier and paraffin, washing gel translucent microballoon carries out serial dehydration with alcohol again and becomes silt shape solid-state-microspherical, then carries out vacuum-drying and become powder.
Purposes of the present invention: for the various traumatic surface of a wound, the antibacterial anti hemorrhagic of the surface of a wound such as mucous membrane, the uterine neck surface of a wound, empyrosis wound surface, operative incision, ulcer, bedsore, promotion organization healing, prevention is adhered and reduces scar.
Tool of the present invention has the following advantages:
1. chitosan has the biological activity such as good biocompatibility and anti-inflammatory, anti, antibacterial, wound healing, can be degraded by the enzyme lysozyme as the available monose of body, show good biodegradability, and play Blood clotting by number of ways.The chitosan quaternary ammonium salt sterilization effect added is better than chitosan, and biocompatibility is better, adds the antibacterial ability of particulate.
2. generate Ah-ACMS complex microsphere after emulsification and cross linked, and alginate calcium has good biocompatibility and biodegradability, and antibacterial, hemostasis, short more, the specific function such as lessen scar formation.After Lalgine calcium salt contacts with the purulence blood on wound, Lalgine calcium salt with the sodium ion generation ion-exchange in body fluid, can be converted into the gel of stickiness while discharging calcium ion, and a large amount of assemblies of wound surface calcium ion can accelerate surface of a wound hemostasis.
3. the microballoon be prepared from after emulsification and cross linked, particle diameter is tiny, there is larger specific surface area, increase the contact area of material and blood, viscogel shape can be become by water absorption and swelling fast, be attached to the surface of a wound, the capillary vessel that the shutoff surface of a wound breaks, enhance the performance of the promotion erythrocyte aggregation of micro-sphere material itself, keep or expand biomaterial anthemorrhagic performance inherently, and form water gel at the surface of a wound after hemostasis, energy wound healing, simultaneously soft, moistening gelinite provides moist repairing environment for wound, accelerate the growth of granulation tissue and the formation of epithelium, thus make this dressing more effect more short than other similar dressing stronger, antibacterial effect is better, haemostatic effect is better, mechanical property is more excellent.
4. the preparation technology of biological hemostatic dressing of the present invention is simple, and material is easy to get, and is applicable to carry out suitability for industrialized production, has a extensive future.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail:
Embodiment 1
Take class of 18.1g department 80,18.1g tween 80,2.0g stearic acid join in 500ml whiteruss, at 72 DEG C, stir into O(water)/(oil) W emulsion.Getting 0.8g HACC is added in 50ml distilled water, and it is in the acetum of 1% that 0.8g chitosan is dissolved in 50ml volume fraction, both mixings.1.2g sodium alginate is added in 100ml distilled water, and the salts solution of chitosan is joined sodium alginate soln fully stirs into stable colloidal solution, then join in the emulsion of paraffin, rapid stirring 25 minutes, stirring velocity 800-1000 rev/min.Add the calcium chloride solution 40ml that massfraction is 1.6% again, maintain the temperature at 72 DEG C, stirring at low speed 50 minutes, stirring velocity 80-100 rev/min.By mixed solution vacuum filtration in core.By 100ml washed with isopropyl alcohol 3 times, then use 30%, 50%, 70%, 100% gradient alcohol dehydration respectively, vacuum-drying becomes powder.
Embodiment 2
Take class of 18.7g department 80,18.7g tween 80,4.2g stearic acid join in 500ml whiteruss, at 75 DEG C, stir into O(water)/(oil) W emulsion.Getting 1.0g HACC is added in 50ml distilled water, and it is in the acetum of 1% that 1.0g chitosan is dissolved in 50ml volume fraction, both mixings.1.5g sodium alginate is added in 100ml distilled water, and the salts solution of chitosan is joined sodium alginate soln fully stirs into stable colloidal solution, then join in the emulsion of paraffin, rapid stirring 30 minutes, stirring velocity 800-1000 rev/min.Add the calcium chloride solution 40ml that massfraction is 2.0% again, maintain the temperature at 75 DEG C, stirring at low speed 55 minutes, stirring velocity 80-100 rev/min.By mixed solution vacuum filtration in core.By 100ml washed with isopropyl alcohol 3 times, then use 30%, 50%, 70%, 100% gradient alcohol dehydration respectively, vacuum-drying becomes powder.
Embodiment 3
Take class of 18.4g department 80,18.4g tween 80,6.1g stearic acid join in 500ml whiteruss, at 78 DEG C, stir into O(water)/(oil) W emulsion.Getting 1.2g HACC is added in 50ml distilled water, and it is in the acetum of 1% that 1.2g chitosan is dissolved in 50ml volume fraction, both mixings.1.8g sodium alginate is added in 100ml distilled water, and the salts solution of chitosan is joined sodium alginate soln fully stirs into stable colloidal solution, then join in the emulsion of paraffin, rapid stirring 45 minutes, stirring velocity 800-1000 rev/min.Add the calcium chloride solution 40ml that massfraction is 2.4% again, maintain the temperature at 80 DEG C, stirring at low speed 1h, stirring velocity 80-100 rev/min.By mixed solution vacuum filtration in core.By 100ml washed with isopropyl alcohol 3 times, then use 30%, 50%, 70%, 100% gradient alcohol dehydration respectively, vacuum-drying becomes powder.
Embodiment 4
Take class of 18.7g department 80,18.7g tween 80,4.2g stearic acid join in 500ml whiteruss, at 75 DEG C, stir into O(water)/(oil) W emulsion.Get 2.0g HACC to be dissolved in 100ml distilled water and to mix, 1.5g sodium alginate is added in 100ml distilled water, and the salts solution of chitosan is joined sodium alginate soln fully stir into stable colloidal solution, join again in the emulsion of paraffin, rapid stirring 30 minutes, stirring velocity 800-1000 rev/min.Adding massfraction is again 2.0% calcium chloride solution 40ml, maintains the temperature at 75 DEG C, stirring at low speed 55 minutes, stirring velocity 80-100 rev/min.By mixed solution vacuum filtration in core.By 100ml washed with isopropyl alcohol 3 times, then use 30%, 50%, 70%, 100% gradient alcohol dehydration respectively, vacuum-drying becomes powder.
Embodiment 5
Take class of 18.7g department 80,18.7g tween 80,4.2g stearic acid join in 500ml whiteruss, at 75 DEG C, stir into O(water)/(oil) W emulsion.Getting 2.0g chitosan, to be added to 100ml volume fraction be in the acetum of 1%.1.5g sodium alginate is added in 100ml distilled water, and the salts solution of chitosan is joined sodium alginate soln fully stirs into stable colloidal solution, then join in the emulsion of paraffin, rapid stirring 30 minutes, stirring velocity 800-1000 rev/min.Adding massfraction is again 2.0% calcium chloride solution 40ml, maintains the temperature at 75 DEG C, stirring at low speed 55 minutes, stirring velocity 80-100 rev/min.By mixed solution vacuum filtration in core.By 100ml washed with isopropyl alcohol 3 times, then use 30%, 50%, 70%, 100% gradient alcohol dehydration respectively, vacuum-drying becomes powder.
HACC of the present invention and chitosan solution have following general formula:
or
Wherein, polymerization degree n is between 300 ~ 2000, and relative molecular mass is 8.4 × 10
4~ 5.6 × 10
5between.
The general structure of Lalgine sodium salt of the present invention is as follows:
Wherein, polymerization degree n is between 180-930, and relative molecular mass is 3.2 × 10
4~ 2.5 × 10
5between.
Purposes of the present invention: for the various traumatic surface of a wound, the antibacterial anti hemorrhagic of the surface of a wound such as mucous membrane, the uterine neck surface of a wound, empyrosis wound surface, operative incision, ulcer, bedsore, promotion organization healing, prevention is adhered and reduces scar.
Claims (3)
1. the preparation method of a marine bioactivity bleeding-stopping dressing, HACC is it is characterized in that to be dissolved in distilled water, be mixed with the aqueous solution that mass percentage concentration is 1.6 ~ 2.4%, chitosan with HACC equivalent being joined volume ratio is in the acetum of 1 ~ 2:100, be mixed with the chitosan solution that mass percentage concentration is 1.6 ~ 2.4%, stir, both are hybridly prepared into the colloidal solution that mass percentage concentration is 1.6 ~ 2.4%, sodium alginate is added in distilled water, be mixed with the micro-yellow milky solution that mass percentage concentration is 1 ~ 2%, then both balanced mix are incited somebody to action as 1 liquid, class 80 of department, tween 80 and stearic acid are mixed into emulsifying agent according to the mass ratio of 9:9:1 ~ 3, and emulsifying agent and whiteruss are mixed according to the mass ratio of 12 ~ 13:125, O/W emulsion is stirred at 72 ~ 78 DEG C, using the emulsion containing paraffin as 2 liquid, 1 liquid and 2 liquid are become stable emulsification system according to the ratio mixing and stirring of 2 ~ 3:5, add linking agent calcium chloride solution and make its crosslinking curing, then be drying to obtain by washing dehydration, described HACC and chitosan solution have following general formula:
or
Wherein, polymerization degree n is between 300 ~ 2000, and relative molecular mass is 8.4 × 10
4~ 5.6 × 10
5between;
The general structure of described Lalgine sodium salt is as follows:
Wherein, polymerization degree n is between 180-930, and relative molecular mass is 3.2 × 10
4~ 2.5 × 10
5between.
2. the preparation method of marine bioactivity bleeding-stopping dressing as claimed in claim 1, it is characterized in that the massfraction of linking agent calcium chloride is 1.6% ~ 3.2%, it joins in emulsification system liquid according to the volume ratio with emulsification system 1:12 ~ 18, the temperature of reaction is 70 ~ 80 DEG C, and the reaction times is 50 ~ 60min.
3. the preparation method of marine bioactivity bleeding-stopping dressing as claimed in claim 1, it is characterized in that described washing is washings washing, described washings is Virahol, except de-emulsifier and paraffin, washing gel translucent microballoon carries out serial dehydration with alcohol again and becomes silt shape solid-state-microspherical, then carries out vacuum-drying and become powder.
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| CN104998293A (en) * | 2015-08-17 | 2015-10-28 | 青岛卫辽医用生物材料有限公司 | Styptic powder |
| CN105031708B (en) * | 2015-08-25 | 2018-03-23 | 东莞市达庆医疗器械有限公司 | A kind of wound rapid hemostasis powder and preparation method thereof |
| CN105169460B (en) * | 2015-10-26 | 2018-09-25 | 广东泰宝医疗科技股份有限公司 | A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof |
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| CN108126236B (en) * | 2017-12-20 | 2021-01-01 | 浙江科技学院 | Medicine coating material and preparation method thereof |
| CN108744022A (en) * | 2018-07-06 | 2018-11-06 | 中国热带农业科学院农产品加工研究所 | A kind of functionality absorbable hemostatic powder and preparation method thereof |
| CN109364288B (en) * | 2018-11-26 | 2021-04-02 | 温州生物材料与工程研究所 | Application of hole-hole composite micro-nano structure polysaccharide microspheres in preparation of hemostatic dressing |
| CN110180018A (en) * | 2019-05-24 | 2019-08-30 | 武汉理工大学 | A kind of preparation method of calcic alginate/chitosan hemostatic microsphere |
| CN111632188A (en) * | 2020-06-09 | 2020-09-08 | 湖北精耕生物工程有限公司 | Seaweed polysaccharide composite biological matrix dressing and preparation method thereof |
| CN112755239A (en) * | 2020-12-08 | 2021-05-07 | 上海市肿瘤研究所 | Composite porous microsphere and preparation method and application thereof |
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| CN1850111A (en) * | 2006-02-28 | 2006-10-25 | 中国人民解放军第二军医大学 | Biodegradeable hemostasis powder |
| CN101735339A (en) * | 2010-01-12 | 2010-06-16 | 浙江理工大学 | Reactive chitosan derivative and preparation method thereof |
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| CN1850111A (en) * | 2006-02-28 | 2006-10-25 | 中国人民解放军第二军医大学 | Biodegradeable hemostasis powder |
| CN101735339A (en) * | 2010-01-12 | 2010-06-16 | 浙江理工大学 | Reactive chitosan derivative and preparation method thereof |
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