CN103446621B - Preparation method of nano-silver-containing sodium alginate based antibacterial medical dressing - Google Patents
Preparation method of nano-silver-containing sodium alginate based antibacterial medical dressing Download PDFInfo
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Abstract
The invention provides a preparation method of a nano-silver-containing sodium alginate based antibacterial medical dressing, and relates to an antibacterial medical dressing with sodium alginate as a main raw material, solving the problems that the air permeability is poor, the wettability is low, the antibacterial performance is not realized and the like in the existing dressing and a preparation method thereof. The medical dressing provided by the invention is composed of silver nanoparticles, sodium alginate and an interpenetrating polymer network film. The preparation method provided by the invention comprises the following steps: I, preparing nano-silver loaded sol; II, preparing a sodium alginate solution loaded with silver nanoparticles; III, preparing a crosslinked polymer solution; IV, preparing a mixing solution used for processing the dressing; V, preliminarily processing and forming the dressing; and VI, preparing the nano-silver-containing sodium alginate based antibacterial medical dressing. The dressing is able to quickly control major bleeding, has antibacterial performance, is outstanding in moisture absorption performance, high in moisture-vapor transmission and biocompatibility, and biodegradable, has the capacity of promoting wound healing and is low in price, simple and convenient to operate, and suitable for mass production.
Description
Technical field
The present invention relates to a kind of take sodium alginate as the antibacterial medical dressing of primary raw material.
Background technology
Skin trauma is clinical common disease, and for avoiding the impact suffering bacteriological infection and other foeign element, protection is wound healing, promotion tissue repair also, and various wound of being everlasting, wound surface cover medical dressing temporarily.Clinical conventional dressing is main mainly with the material such as gauze, medical absorbent cotton, although cost is low, raw material sources are extensive, make simple, there is stronger absorbability, but haemostatic effect is not good enough, without wound surface moisture-keeping function, easily cause secondary damage, the hydrops of wound face easily causes bacteriological infection etc.
Sodium alginate (sodiumalginate) is a kind of linear anionic natural polysaccharide extracted from Sargassum, is polymerized by (1-4) glycosidic bond by beta-D-mannuronic acid (M) and α-L-guluronic acid (G).As a kind of polyanion electrolyte, in its aqueous solution, add Ca
2+, Ba
2+after bivalent cation, the Na on G unit
+with divalent ion generation ion-exchange reactions, G group is piled up and is formed cross-linked network structure, thus changes existing strength character resilient calcium alginate gel again into.Alginate dressing is as a kind of natural plant repair in trauma material, and the wet gel film that can be formed in wound surface, has high-hygroscopicity, ease of removal, have good biocompatibility; There is biodegradability; While guarantee is to antibacterial insulating effect, play the effect of hemostasis and acceleration wound healing.
Chitosan chitosan is the natural polymer straight-chain polysaccharide be prepared from through deacetylation by chitin; chemical name is (1-4)-2-amino-2-deoxidation-callose; it is alkaline polysaccharide unique in natural polysaccharide; because it has good biocompatibility; minimum toxicity, has been widely used in organizational project, artificial skin, slow releasing carrier of medication, gene delivery etc.Chitosan, as wound dressing, has lot of advantages, as the effect, promotion tissue regeneration etc. of hemostasis.
Chinese patent application publication No. is that the patent of CN101816802A provides a kind of chitosan-based medical dressing, is made up of chitosan, oligochitosan and enhancing macromolecule, and what wherein have bacteriostasis is chitosan, but it exists the not strong problem of anti-microbial property.There is the problem that poor mechanical property, permeability are not good and wettability is not strong in existing chitosan-based medical dressing and preparation method thereof.
Summary of the invention
The object of the invention is to there is anti-microbial property and wettability is poor, the strong problem of breathability and mechanical property to solve existing chitosan-based medical dressing and preparation method thereof, and provide a kind of preparation method of the alginic acid sodio antibacterial medical dressing containing nanometer silver.
A kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of the present invention is made up of nano silver particles and interpenetrating network membrane (ipn); Wherein, described interpenetrating network membrane (ipn) is made up of sodium alginate and macromolecule, and described nano silver particles and the mol ratio of sodium alginate are 1:10 ~ 50; Nano silver particles and high molecular mol ratio are 1 ~ 10:60.
The preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of the present invention is carried out according to following steps:
One, preparation is loaded with nano silver colloidal sol: by silver nitrate deionized water dissolving, obtain the silver nitrate aqueous solution that molar concentration is 10 ~ 100mM; Joined by sodium alginate in silver nitrate aqueous solution, sodium alginate and silver nitrate molar concentration rate are 1 ~ 5:1, stirring at room temperature 1 hour, must contain the silver nitrate solution of sodium alginate; Joined by reducing agent in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
Two, preparation is loaded with the sodium alginate soln of nano silver particles: joined by sodium alginate in the obtained nano silver colloidal sol of step one, obtain the sodium alginate nano silver colloidal sol that concentration is 1 ~ 3.5%W/V, stirring at room temperature is after 4 hours, room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
Three, prepare high molecular crosslink solution: by macromolecule dissolution with solvents, obtaining concentration is 1 ~ 15%W/V macromolecular solution, is joined by glutaraldehyde in macromolecular solution, and secure satisfactory grades sub-crosslinker solution; Wherein, the volume ratio of glutaraldehyde and high molecular crosslink solution is 1 ~ 8:100;
Four, the mixed solution of formulated dressing: the sodium alginate soln containing nano silver particles adding step 2 gained in the high molecular crosslink solution of step 3 gained, after stirring, must process the mixed solution of dressing; Wherein, high molecular crosslink solution is 1:1 ~ 10 with the volume ratio of the sodium alginate soln containing nano silver particles;
Five, dressing initial processing molding: the mixed solution of the processing dressing of four gained is poured in culture dish, drying and forming-film;
Six, prepare interpenetrating network membrane (ipn): by the film of five gained, being immersed in mass percentage is in 3% ~ 8% calcium chloride solution, crosslinking curing 15 ~ 30 time, obtains interpenetrating network membrane (ipn); After deionized water rinsing, drying at room temperature, obtains the alginic acid sodio antibacterial medical dressing containing nanometer silver;
Wherein, the sodium alginate described in step one and silver nitrate mol ratio are 1 ~ 5:1, and reducing agent is 0.5 ~ 3:1 with the mol ratio of the silver nitrate solution containing sodium alginate.
Of the present inventionly comprise following beneficial effect:
Dressing of the present invention has carried out morphology observation test, and dressing pore diameter range is at 100-800 micron.
Dressing good toughness of the present invention, can bend arbitrarily, to be suitable for different parts wound.
When antibacterial medical dressing of the present invention contacts with wound, the calcium ion of release can and induced platelet activation, produce hemostasis somatomedin, play hemostasis and accelerate the effect of wound healing, control rapidly severe haemorrhage efficiently, there is certain pliability and intensity; Be combined with wound surface and not with wound surface adhesion, avoid secondary damage.
Dressing of the present invention has carried out air penetrability test, and air penetrability is 85-120g/ (m
2h), the air penetrability of desirable dressing is met.
Dressing of the present invention has carried out transmission scan test, and the nano silver particles size be loaded with is at 20-100nm, and the nano silver particles of this rank has higher bactericidal activity, has obvious inhibitory action to most of mushroom.
Dressing of the present invention has carried out mechanical stretch test, and maximum disruptive force and hot strength are respectively 40N and 100MPa;
Dressing good biocompatibility of the present invention, biodegradable;
Dressing of the present invention has stronger water absorbing capacity, and moisture pick-up properties is excellent;
Dressing good stability of the present invention, easy and simple to handle, cheap, be suitable for large-scale production.
Detailed description of the invention
Detailed description of the invention one: a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of present embodiment is made up of nano silver particles and interpenetrating network membrane (ipn); Wherein, described interpenetrating network membrane (ipn) is made up of sodium alginate and macromolecule, and described nano silver particles and the mol ratio of sodium alginate are 1:10 ~ 50; Nano silver particles and high molecular mol ratio are 1 ~ 10:60.
The dressing of present embodiment has carried out morphology observation test, and dressing pore diameter range is at 100-800 micron.
The dressing good toughness of present embodiment, can bend arbitrarily, to be suitable for different parts wound.
When the antibacterial medical dressing of present embodiment contacts with wound, the calcium ion of release can and induced platelet activation, produce hemostasis somatomedin, play hemostasis and accelerate the effect of wound healing, control severe haemorrhage efficiently rapidly, there is certain pliability and intensity; Be combined with wound surface and not with wound surface adhesion, avoid secondary damage.
The dressing of present embodiment has carried out air penetrability test, and air penetrability is 85-120g/ (m
2h), the air penetrability of desirable dressing is met.
The dressing of present embodiment has carried out transmission scan test, and the nano silver particles size be loaded with is at 20-100nm, and the nano silver particles of this rank has higher bactericidal activity, has obvious inhibitory action to most of mushroom.
The dressing of present embodiment has carried out mechanical stretch test, and maximum disruptive force and hot strength are respectively 40N and 100MPa;
The dressing good biocompatibility of present embodiment, biodegradable;
The dressing of present embodiment has stronger water absorbing capacity, and moisture pick-up properties is excellent;
The dressing good stability of present embodiment, easy and simple to handle, cheap, be suitable for large-scale production.
Detailed description of the invention two: present embodiment and detailed description of the invention one unlike: macromolecule be in gelatin, polyvinyl alcohol or chitosan one or more by the mixture of any combination.Other is identical with detailed description of the invention one.
Detailed description of the invention three: present embodiment and detailed description of the invention one or two unlike: the mol ratio of nano silver particles and sodium alginate is 1 ~ 5:10.Other is identical with detailed description of the invention one or two.
Detailed description of the invention four: one of present embodiment and detailed description of the invention one to three unlike: the mol ratio of nano silver particles and macromolecule interpenetrating network membrane (ipn) is 1::10 ~ 60.Other is identical with one of detailed description of the invention one to three.
Detailed description of the invention five: the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of present embodiment, concrete operation step is as follows:
One, preparation is loaded with nano silver colloidal sol: by silver nitrate deionized water dissolving, obtain the silver nitrate aqueous solution that molar concentration is 10 ~ 100mM; Joined by sodium alginate in silver nitrate aqueous solution, sodium alginate and silver nitrate molar concentration rate are 1 ~ 5:1, stirring at room temperature 1 hour, must contain the silver nitrate solution of sodium alginate; Joined by reducing agent in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
Two, preparation is loaded with the sodium alginate soln of nano silver particles: joined by sodium alginate in the obtained nano silver colloidal sol of step one, obtain the sodium alginate nano silver colloidal sol that concentration is 1 ~ 3.5%W/V, stirring at room temperature is after 4 hours, room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
Three, prepare high molecular crosslink solution: by macromolecule dissolution with solvents, obtaining concentration is 1 ~ 15%W/V macromolecular solution, is joined by glutaraldehyde in macromolecular solution, and secure satisfactory grades sub-crosslinker solution; Wherein, the volume ratio of glutaraldehyde and high molecular crosslink solution is 1 ~ 8:100;
Four, the mixed solution of formulated dressing: the sodium alginate soln containing nano silver particles adding step 2 gained in the high molecular crosslink solution of step 3 gained, after stirring, must process the mixed solution of dressing; Wherein, high molecular crosslink solution is 1:1 ~ 10 with the volume ratio of the sodium alginate soln containing nano silver particles;
Five, dressing initial processing molding: the mixed solution of the processing dressing of four gained is poured in culture dish, drying and forming-film;
Six, prepare interpenetrating network membrane (ipn): by the film of five gained, being immersed in mass percentage is in 3% ~ 8% calcium chloride solution, crosslinking curing 15 ~ 30 time, obtains interpenetrating network membrane (ipn); After deionized water rinsing, drying at room temperature, obtains the alginic acid sodio antibacterial medical dressing containing nanometer silver;
Wherein, the sodium alginate described in step one and silver nitrate mol ratio are 1 ~ 5:1, and reducing agent is 0.5 ~ 3:1 with the mol ratio of the silver nitrate solution containing sodium alginate.
The dressing of present embodiment has carried out morphology observation test, and dressing pore diameter range is at 100-800 micron.
The dressing good toughness of present embodiment, can bend arbitrarily, to be suitable for different parts wound.
When the antibacterial medical dressing of present embodiment contacts with wound, the calcium ion of release can and induced platelet activation, produce hemostasis somatomedin, play hemostasis and accelerate the effect of wound healing, control severe haemorrhage efficiently rapidly, there is certain pliability and intensity; Be combined with wound surface and not with wound surface adhesion, avoid secondary damage.
The dressing of present embodiment has carried out air penetrability test, and air penetrability is 85-120g/ (m
2h), the air penetrability of desirable dressing is met.
The dressing of present embodiment has carried out transmission scan test, and the nano silver particles size be loaded with is at 20-100nm, and the nano silver particles of this rank has higher bactericidal activity, has obvious inhibitory action to most of mushroom.
The dressing of present embodiment has carried out mechanical stretch test, and maximum disruptive force and hot strength are respectively 40N and 100MPa;
The dressing good biocompatibility of present embodiment, biodegradable;
The dressing of present embodiment has stronger water absorbing capacity, and moisture pick-up properties is excellent;
The dressing good stability of present embodiment, easy and simple to handle, cheap, be suitable for large-scale production.
Detailed description of the invention six: present embodiment and detailed description of the invention five are unlike the reducing agent glucose described in step one or hydrazine hydrate.Other is identical with detailed description of the invention five.
Detailed description of the invention seven: present embodiment and detailed description of the invention five or six unlike: the silver nitrate aqueous solution concentration described in step one is 50mM.Other is identical with detailed description of the invention five or six.
Detailed description of the invention eight: one of present embodiment and detailed description of the invention five to seven unlike: the sodium alginate described in step one and silver nitrate mol ratio are 2.5:1.Other is identical with one of detailed description of the invention five to seven.
Detailed description of the invention nine: one of present embodiment and detailed description of the invention five to eight unlike: the sodium alginate nano silver colloidal sol described in step 2 is 1.5 ~ 3%W/V.Other is identical with one of detailed description of the invention five to eight.
Detailed description of the invention ten: one of present embodiment and detailed description of the invention five to nine unlike: the volume ratio of the glutaraldehyde described in step 3 and high molecular crosslink solution is 4:100.Other is identical with one of detailed description of the invention five to nine.
Detailed description of the invention 11: one of present embodiment and detailed description of the invention five to ten unlike: the macromolecular solution described in step 3 is 5 ~ 10%W/V.Other is identical with one of detailed description of the invention five to ten.
Detailed description of the invention 12: one of present embodiment and detailed description of the invention five to ten one are 1:3 unlike: the high molecular crosslink solution described in step 4 and the volume ratio of the sodium alginate soln containing nano silver particles.Other is identical with one of detailed description of the invention five to ten one.
Detailed description of the invention 13: one of present embodiment and detailed description of the invention five to ten two unlike: the macromolecule described in step 3 be in gelatin, polyvinyl alcohol or chitosan one or more by the mixture of any combination.Other is identical with one of detailed description of the invention five to ten two.
Detailed description of the invention 14: one of present embodiment and detailed description of the invention five to ten three unlike: the solvent described in step 3 is deionized water or acetic acid.Other is identical with one of detailed description of the invention five to ten three.
The present invention is further described to provide embodiments of the invention below, instead of limit the scope of the invention.
Embodiment 1
The preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of the present embodiment, concrete operation step is as follows:
(1) preparation is loaded with nano silver colloidal sol: by silver nitrate 3 grams, dissolves, obtain silver nitrate solution by 100 ml deionized water; By sodium alginate 2 grams, join in above-mentioned silver nitrate solution, stirring at room temperature 1 hour, the silver nitrate solution of sodium alginate must be contained; By reducing agent glucose 10 grams, join in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
(2) preparation is loaded with the sodium alginate soln of nano silver particles: by 2 grams of sodium alginates, join in step (1) nano silver colloidal sol, stirring at room temperature is after 4 hours, and room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
(3) prepare chitosan crosslinked solution: take chitosan 3 grams, get 100 milliliters of acetic acid, stirring at room temperature is to dissolving completely, and add 1 milliliter of glutaraldehyde, stirring at room temperature 12 hours, obtains chitosan crosslinked solution;
(4) the mixed solution of formulated dressing: the chitosan crosslinked solution getting 10 milliliters of step (3) gained, add the sodium alginate soln containing nano silver particles of 20 milliliters of step (2) gained, stirring at room temperature 1 hour, must process the mixed solution of dressing;
(5) dressing initial processing molding: the mixed solution of the processing dressing of (4) gained is poured in culture dish, at room temperature drying and forming-film;
(6) prepare the dressing of interpenetrating networks: by the film of (5) gained, put into 500 milliliters of 6%W/V calcium chloride solutions, crosslinking curing 30 minutes, rinses rear drying at room temperature repeatedly with deionized water;
(7) as required the material obtained in (6) is cut out or is processed into the medical accessory of different size;
(8) encapsulate, sterilizing.
The alginic acid sodio antibacterial medical dressing pore diameter range containing nanometer silver that the present embodiment obtains is at 100 ~ 800 microns); Air penetrability is 80 ~ 120g/ (m
2h); The nano silver particles size be loaded with is at 20 ~ 80nm; The maximum disruptive force of alginic acid sodio antibacterial medical dressing containing nanometer silver that the present embodiment obtains and hot strength are respectively 40N and 100MPa;
The dressing good biocompatibility of the present embodiment, biodegradable;
The dressing of the present embodiment has stronger water absorbing capacity, and moisture pick-up properties is excellent;
The dressing good stability of the present embodiment, easy and simple to handle, cheap, be suitable for large-scale production.
Embodiment 2
The preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of the present embodiment, concrete operation step is as follows:
(1) preparation is loaded with nano silver colloidal sol: by silver nitrate 3.4 grams, dissolves, obtain silver nitrate solution by 100 ml deionized water; Sodium alginate 2 grams is joined in above-mentioned silver nitrate solution, stirring at room temperature 1 hour, the silver nitrate solution of sodium alginate must be contained; By borane reducing agent sodium hydride 0.2 gram, join in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
(2) preparation is loaded with the sodium alginate soln of nano silver particles: by 4 grams of sodium alginates, join in step (1) nano silver colloidal sol, stirring at room temperature is after 4 hours, and room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
(3) prepare polyvinyl alcohol crosslinked solution: weighing polyvinyl alcohol 3 grams, get 100 ml deionized water, be stirred in 90 degrees Celsius and dissolve completely, add 6 milliliters of glutaraldehydes, stirring at room temperature 5 hours, obtains polyvinyl alcohol crosslinked solution;
(4) the mixed solution of formulated dressing: the polyvinyl alcohol crosslinked solution getting 10 milliliters of step (3) gained, add the sodium alginate soln containing nano silver particles of 20 milliliters of step (2) gained, stirring at room temperature 1 hour, must process the mixed solution of dressing;
(5) dressing initial processing molding: the mixed solution of the processing dressing of (4) gained is poured in culture dish, at room temperature drying and forming-film;
(6) prepare the dressing of interpenetrating networks: by the film of (5) gained, put into 500 milliliters of 6%W/V calcium chloride solutions, crosslinking curing 30 minutes, rinses rear drying at room temperature repeatedly with deionized water;
(7) as required the material obtained in (6) is cut out or is processed into the medical accessory of different size;
(8) encapsulate, sterilizing.
The alginic acid sodio antibacterial medical dressing pore diameter range containing nanometer silver that the present embodiment obtains is at 100 ~ 1000 microns; Air penetrability is 80 ~ 100g/ (m
2h); The nano silver particles size be loaded with is at 20 ~ 80nm; The maximum disruptive force of alginic acid sodio antibacterial medical dressing containing nanometer silver that the present embodiment obtains and hot strength are respectively 40N and 100MPa;
The dressing good biocompatibility of the present embodiment, biodegradable;
The dressing of the present embodiment has stronger water absorbing capacity, and moisture pick-up properties is excellent;
The dressing good stability of the present embodiment, easy and simple to handle, cheap, be suitable for large-scale production.
Embodiment 3
The preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver of the present embodiment, concrete operation step is as follows:
(1) preparation is loaded with nano silver colloidal sol: by silver nitrate 1.7 grams, dissolves, obtain silver nitrate solution by 100 ml deionized water; Sodium alginate 2 grams is joined in above-mentioned silver nitrate solution, stirring at room temperature 1 hour, the silver nitrate solution of sodium alginate must be contained; By borane reducing agent sodium hydride 0.2 gram, join in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
(2) preparation is loaded with the sodium alginate soln of nano silver particles: by 2 grams of sodium alginates, join in step (1) nano silver colloidal sol, stirring at room temperature is after 4 hours, and room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
(3) prepare gelatin crosslinker solution: take 3 grams, gelatin, get 100 ml deionized water, be stirred in 50 degrees Celsius and dissolve completely, add 0.1 milliliter of glutaraldehyde, stirring at room temperature 3 hours, obtains gelatin crosslinker solution;
(4) the mixed solution of formulated dressing: the gelatin crosslinker solution getting 10 milliliters of step (3) gained, add the sodium alginate soln containing nano silver particles of 20 milliliters of step (2) gained, stirring at room temperature 1 hour, must process the mixed solution of dressing;
(5) dressing initial processing molding: the mixed solution of the processing dressing of (4) gained is poured in culture dish, at room temperature drying and forming-film;
(6) prepare the dressing of interpenetrating networks: by the film of (5) gained, put into 500 milliliters of 5%W/V calcium chloride solutions, crosslinking curing 30 minutes, rinses rear drying at room temperature repeatedly with deionized water;
(7) as required the material obtained in (6) is cut out or is processed into the medical accessory of different size;
(8) encapsulate, sterilizing.
The alginic acid sodio antibacterial medical dressing pore diameter range containing nanometer silver that the present embodiment obtains is at 100 ~ 800 microns; Air penetrability is 80 ~ 120g/ (m
2h); The nano silver particles size be loaded with is at 20 ~ 100nm; The maximum disruptive force of alginic acid sodio antibacterial medical dressing containing nanometer silver that the present embodiment obtains and hot strength are respectively 40N and 100MPa;
The dressing good biocompatibility of the present embodiment, biodegradable;
The dressing of the present embodiment has stronger water absorbing capacity, and moisture pick-up properties is excellent;
The dressing good stability of the present embodiment, easy and simple to handle, cheap, be suitable for large-scale production.
Claims (7)
1., containing a preparation method for the alginic acid sodio antibacterial medical dressing of nanometer silver, it is characterized in that the preparation method of the described alginic acid sodio antibacterial medical dressing containing nanometer silver is carried out according to following steps:
One, preparation is loaded with nano silver colloidal sol: by silver nitrate deionized water dissolving, obtain the silver nitrate aqueous solution that molar concentration is 10 ~ 100mM; Joined by sodium alginate in silver nitrate aqueous solution, sodium alginate and silver nitrate molar concentration rate are 1 ~ 5:1, stirring at room temperature 1 hour, must contain the silver nitrate solution of sodium alginate; Joined by reducing agent in the silver nitrate solution containing sodium alginate, stirring at room temperature 30 minutes, obtains nano silver colloidal sol;
Two, preparation is loaded with the sodium alginate soln of nano silver particles: joined by sodium alginate in the obtained nano silver colloidal sol of step one, obtain the sodium alginate nano silver colloidal sol that concentration is 1 ~ 3.5%W/V, stirring at room temperature is after 4 hours, room temperature leaves standstill deaeration in 24 hours, must contain the sodium alginate soln of nano silver particles;
Three, prepare high molecular crosslink solution: by macromolecule dissolution with solvents, obtaining concentration is 1 ~ 15%W/V macromolecular solution, is joined by glutaraldehyde in macromolecular solution, and secure satisfactory grades sub-crosslinker solution; Wherein, the volume ratio of glutaraldehyde and high molecular crosslink solution is 1 ~ 8:100;
Four, the mixed solution of formulated dressing: the sodium alginate soln containing nano silver particles adding step 2 gained in the high molecular crosslink solution of step 3 gained, after stirring, must process the mixed solution of dressing; Wherein, high molecular crosslink solution is 1:1 ~ 10 with the volume ratio of the sodium alginate soln containing nano silver particles;
Five, dressing initial processing molding: the mixed solution of the processing dressing of four gained is poured in culture dish, drying and forming-film;
Six, prepare interpenetrating network membrane (ipn): by the film of five gained, being immersed in mass percentage is in 3% ~ 8% calcium chloride solution, and crosslinking curing 15 ~ 30 minutes, obtains interpenetrating network membrane (ipn); After deionized water rinsing, drying at room temperature, obtains the alginic acid sodio antibacterial medical dressing containing nanometer silver;
Wherein, the sodium alginate described in step one and silver nitrate mol ratio are 1 ~ 5:1, and reducing agent is 0.5 ~ 3:1 with the mol ratio of the silver nitrate solution containing sodium alginate; Macromolecule described in step 3 be in gelatin, polyvinyl alcohol or chitosan one or more by the mixture of any combination.
2. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the reducing agent described in step one is glucose, sodium borohydride or hydrazine hydrate.
3. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the silver nitrate aqueous solution concentration described in step one is 50mM.
4. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the sodium alginate described in step one and silver nitrate mol ratio are 2.5:1.
5. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the sodium alginate nano silver colloidal sol described in step 2 is 1.5 ~ 3%W/V.
6. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the solvent described in step 3 is deionized water or acetic acid.
7. the preparation method of a kind of alginic acid sodio antibacterial medical dressing containing nanometer silver according to claim 1, is characterized in that the macromolecular solution described in step 3 is 5 ~ 10%W/V.
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