CN112933289A - Preparation method of sodium alginate grafted tea polyphenol antibacterial dressing - Google Patents
Preparation method of sodium alginate grafted tea polyphenol antibacterial dressing Download PDFInfo
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- CN112933289A CN112933289A CN202110134899.6A CN202110134899A CN112933289A CN 112933289 A CN112933289 A CN 112933289A CN 202110134899 A CN202110134899 A CN 202110134899A CN 112933289 A CN112933289 A CN 112933289A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0038—Gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The invention provides a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol, which comprises the steps of covalently grafting the tea polyphenol with sodium alginate, then flatly spreading and solidifying a mixed solution of a sodium alginate grafted tea polyphenol copolymer, gelatin and glycerol in a metal calcium ion solution, and finally further solidifying a gel film in an organic acid solution of chitosan to prepare the antibacterial dressing of the sodium alginate grafted tea polyphenol. The antibacterial dressing has the advantages of strong antibacterial performance, good air permeability, biodegradability, simple operation, easy mass production and capability of providing guidance for the research of medical gel dressings.
Description
Technical Field
The invention belongs to the technical field of preparation of medical gel dressings, and particularly relates to a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol.
Background
Skin wounds are common clinical diseases, and medical dressings are covered on the surfaces of various wounds and wounds temporarily to avoid bacterial infection. The common clinical dressing mainly comprises gauze, medical absorbent cotton and other materials, has low cost, wide raw material sources and simple preparation, but has poor hemostatic effect, does not have wound moisturizing effect, is easy to cause bacterial infection and the like caused by secondary injury. Therefore, it is necessary to develop a medical dressing with good moisture retention effect and excellent antibacterial performance.
Sodium alginate (sodium alginate) is used as polyanionic electrolyte, calcium ions are added into the aqueous solution of the polyanionic electrolyte to carry out ion exchange reaction, and the calcium alginate is converted into calcium alginate gel with strength and elasticity. The alginate dressing is used as a natural plant wound repair material, can form a wet gel film on the surface of a wound, has high hygroscopicity and easy removability, and has good biocompatibility; has the biodegradability; the hemostatic and wound healing accelerating effects are achieved while the bacteria isolation effect is ensured. Tea polyphenols are phenolic compounds extracted from tea leaves, and have been widely used in various industries because of their natural safety and good antibacterial and antioxidant activities. The antibacterial performance of the sodium alginate can be improved by grafting the tea polyphenol to the surface of the sodium alginate. Meanwhile, chitosan is inserted into the sodium alginate-based gel to be used as a wound dressing, so that the effects of stopping bleeding, promoting tissue regeneration and the like can be achieved.
CN101816802A provides a chitosan-based medical dressing, which is composed of chitosan, chitosan oligosaccharide and reinforced macromolecules, wherein the chitosan has an antibacterial effect, but the problem of weak antibacterial performance exists. Therefore, the research of the medical dressing which takes the sodium alginate as the substrate and has excellent antibacterial and moisturizing performances has practical significance.
Disclosure of Invention
In order to solve the problems of weak antibacterial performance, poor air permeability and weak water absorption of the conventional medical dressing, the invention provides a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol.
The invention is realized by the following technical scheme:
a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol comprises the following steps:
(1) preparation of sodium alginate grafted tea polyphenol copolymer (TP/SA)
Dissolving sodium alginate in acetic acid solution, stirring at room temperature to dissolve; and then weighing tea polyphenol, dissolving in water, quickly adding into the solution, mechanically stirring at 80-90 ℃ for reaction for 10-12 h, and cooling to room temperature to obtain a sodium alginate grafted tea polyphenol copolymer (TP/SA) solution.
The mass volume ratio of the sodium alginate to the acetic acid solution is 1 g: (50-70 mL); wherein the mass fraction of the acetic acid solution is 5-8%.
The mass ratio of the sodium alginate to the tea polyphenol is 1 g: 1.7-2.5 g.
(2) Preparation of sodium alginate grafted tea polyphenol antibacterial dressing
Adding gelatin and glycerol into the tea polyphenol grafted sodium alginate complex (TP/SA) solution prepared in the step (1), degassing and casting at 35-40 ℃, and then adding calcium chloride metal solution to solidify for 2-3 h to obtain the formed gel film. And then dissolving chitosan in an organic acid solvent at the temperature of 40-42 ℃ to form a chitosan acid solution, wherein the pH value is 4.0-4.5. And finally, soaking the gel film in chitosan acid liquor for 8-10 hours to form the solid sodium alginate grafted tea polyphenol antibacterial dressing.
The addition of the glycerol can effectively improve the moisturizing performance of the antibacterial dressing; the chitosan acid solution is soaked in the gel film, so that the tensile property of the film can be improved, and the hemostatic and antibacterial capabilities of the gel dressing are enhanced.
The mass ratio of the tea polyphenol grafted sodium alginate complex (TP/SA), gelatin and glycerol is (4-6) g: (3-5) g: 1g of the total weight of the composition.
The degassing casting process comprises the following steps: degassing at vacuum degree of not less than-0.090 MPa; and pouring the formed slurry onto a 20 cm-by-20 cm dry and clean glass dish for casting to form a film, wherein the thickness of the slurry is controlled to be 0.05-0.1 cm.
The mass fraction of the calcium chloride metal solution is 2-3%.
The mass-volume ratio of the chitosan to the organic acid is 1 g: 20-40 mL; wherein the organic acid can be one of acetic acid, citric acid and malic acid.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, natural biomass sodium alginate is used as a substrate, and tea polyphenol is introduced into the dressing through graft modification, so that the dressing material has excellent antibacterial performance.
2. The raw materials of the invention are all green reagents, have no pollution to the environment and have the characteristic of environmental protection.
3. The preparation method is simple, does not need any harmful reagent, and is easy for large-scale production and application.
Drawings
Fig. 1 is a photograph showing the appearance of the antibacterial dressing of sodium alginate grafted tea polyphenol prepared in example.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
example 1:
a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol comprises the following steps:
(1) preparation of sodium alginate grafted tea polyphenol copolymer (TP/SA)
Weighing 5g of sodium alginate, dissolving the sodium alginate in 250mL of 5% acetic acid solution, and stirring and dissolving the sodium alginate at room temperature; then weighing 8.5g of tea polyphenol, dissolving in 20mL of water, quickly adding into the solution, mechanically stirring at 90 ℃ for reaction for 10h, and cooling to room temperature to obtain a sodium alginate grafted tea polyphenol copolymer (TP/SA) solution.
(2) Preparation of sodium alginate grafted tea polyphenol antibacterial dressing
And (2) adding 5g of gelatin and 1g of glycerol into 50mL of the tea polyphenol grafted sodium alginate complex (TP/SA) solution prepared in the step (1), degassing at 40 ℃, pouring the solution onto a dry and clean glass dish of 20cm multiplied by 20cm for casting to form a film, and controlling the thickness of the slurry to be 0.05 cm. Then adding 50mL of 2% calcium chloride metal solution to solidify for 3h to obtain the formed gel film. Then 1g of chitosan was dissolved in 20mL of acetic acid at 40 ℃ to form a chitosan acid solution, pH 4.0. And finally, taking out the gel film, and soaking in chitosan acid liquor for 10 hours to form the solid sodium alginate grafted tea polyphenol antibacterial dressing.
Example 2:
a preparation method of an antibacterial dressing of sodium alginate grafted tea polyphenol comprises the following steps:
(1) preparation of sodium alginate grafted tea polyphenol copolymer (TP/SA)
Weighing 4g of sodium alginate, dissolving the sodium alginate in 200mL of 8% acetic acid solution, and stirring and dissolving at room temperature; then weighing 8.0g of tea polyphenol, dissolving in 20mL of water, quickly adding into the solution, mechanically stirring at 80 ℃ for reaction for 12h, and cooling to room temperature to obtain a sodium alginate grafted tea polyphenol copolymer (TP/SA) solution.
(2) Preparation of sodium alginate grafted tea polyphenol antibacterial dressing
And (2) adding 4g of gelatin and 1g of glycerol into 50mL of the tea polyphenol grafted sodium alginate complex (TP/SA) solution prepared in the step (1), degassing at 40 ℃, pouring the solution onto a dry and clean glass dish of 20cm multiplied by 20cm for casting to form a film, and controlling the thickness of the slurry to be 0.1 cm. Then adding 50mL of 2% calcium chloride metal solution to solidify for 3h to obtain the formed gel film. Then 1g of chitosan was dissolved in 20mL of citric acid at 40 ℃ to form a chitosan acid solution with a pH of 4.0. And finally, taking out the gel film, and soaking in chitosan acid liquor for 10 hours to form the solid sodium alginate grafted tea polyphenol antibacterial dressing.
Comparative example:
a preparation method of sodium alginate-based antibacterial dressing, in the comparative example, no sodium alginate graft tea polyphenol copolymer is synthesized, and the preparation method comprises the following steps:
(1) preparation of sodium alginate solution
5g of sodium alginate is weighed and dissolved in 250mL of 5% acetic acid solution, and the solution is stirred and dissolved at room temperature to obtain sodium alginate solution.
(2) Preparation of sodium alginate based antibacterial dressing
And (2) adding 5g of gelatin and 1g of glycerol into 50mL of sodium alginate solution prepared in the step (1), degassing at 35-40 ℃, pouring the mixture onto a dry and clean glass dish of 20cm multiplied by 20cm for casting to form a film, and controlling the thickness of the slurry to be 0.05 cm. Then adding 50mL of 2% calcium chloride metal solution to solidify for 3h to obtain the formed gel film. Then 1g of chitosan was dissolved in 20mL of acetic acid at 40 ℃ to form a chitosan acid solution, pH 4.0. And finally, taking out the gel film, and soaking the gel film in chitosan acid liquor for 10 hours to form the solid sodium alginate-based antibacterial dressing.
Test example 1:
and (3) testing the appearance of the antibacterial dressing of the sodium alginate grafted tea polyphenol.
Test samples: the sodium alginate grafted tea polyphenol antibacterial dressing prepared in the example.
And (3) morphology analysis: fig. 1 is a photograph showing the appearance of the antibacterial dressing of sodium alginate grafted tea polyphenol prepared in the example. The appearance of the prepared sodium alginate grafted tea polyphenol antibacterial dressing is semitransparent gel, the surface is smooth and light, and the skin can be easily attached.
Test example 2:
and testing the tensile property of the antibacterial dressing of the sodium alginate grafted tea polyphenol.
Test samples: the antibacterial dressing of sodium alginate grafted tea polyphenol prepared in step (2) in examples 1 and 2 and the antibacterial dressing of sodium alginate base prepared in step (2) in the comparative example.
The breaking elongation of the sodium alginate-grafted tea polyphenol antibacterial dressings prepared in examples and the sodium alginate-based antibacterial dressings prepared in comparative examples was measured, samples of the antibacterial dressings were cut into a rectangular shape of 1cm × 5cm, the thickness of each sample was measured using a micrometer screw, and the average thickness was calculated. The elongation at break (EB,%) of the film samples was measured using a texture analyzer with an initial length set at 3cm and a test speed set at 25 mm/min. The specific test results are shown in table 1.
Table 1 elongation at break test data table
The breaking elongation of the antibacterial dressing prepared by the sodium alginate grafted tea polyphenol in the embodiment and the breaking elongation of the antibacterial dressing prepared by the sodium alginate-based tea polyphenol in the comparative example are compared, and the result shows that the breaking elongation of the antibacterial dressing prepared by the sodium alginate grafted tea polyphenol in the embodiment is higher, so that the tensile property of the antibacterial dressing can be effectively enhanced through the graft modification of the tea polyphenol.
Test example 3:
and (3) testing the antibacterial performance of the antibacterial dressing of the sodium alginate grafted tea polyphenol.
Test samples: the antibacterial dressing of sodium alginate grafted tea polyphenol prepared in step (2) in examples 1 and 2 and the antibacterial dressing of sodium alginate base prepared in step (2) in the comparative example.
The antibacterial performance of the sodium alginate grafted tea polyphenol antibacterial dressing prepared in the example and the sodium alginate-based antibacterial dressing prepared in the comparative example are tested, staphylococcus aureus (S.aureus) and escherichia coli (E.coil) are used as tested bacteria, are dropwise and uniformly distributed on an antibacterial film, and the number of surviving colonies is recorded after the antibacterial dressing is cultured for 24 hours at 37 ℃. The formula for calculating the Antibacterial Ratio (AR) is as follows:
AR＝(N0-N)/N0×100％
in the formula, N0The initial average number of tested bacteria on the corn straw cellulose antibacterial film is obtained; n is the average number of tested bacteria on the corn stalk cellulose antibacterial film. Each sample was tested in 3 groups and averaged. The test results are shown in Table 2 by referring to GB/T21510-2008.
TABLE 2 antibacterial Property test data sheet
As seen from the data in Table 2, the antibacterial dressing of the sodium alginate grafted tea polyphenol prepared in the example has excellent antibacterial performance; the overall result shows that the antibacterial dressing prepared by the preparation method has excellent antibacterial performance, and the highest sterilization rate of the dressing to escherichia coli can reach 99.4%; the highest sterilization rate to staphylococcus aureus can reach 98.4%.
Claims (7)
1. A preparation method of sodium alginate grafted tea polyphenol antibacterial dressing is characterized by comprising the following steps:
(1) preparation of sodium alginate grafted tea polyphenol copolymer
Dissolving sodium alginate in acetic acid solution, stirring at room temperature to dissolve; then weighing tea polyphenol, dissolving in water, quickly adding into the solution, mechanically stirring at 80-90 ℃ for reaction for 10-12 h, and cooling to room temperature to obtain a sodium alginate grafted tea polyphenol copolymer solution;
(2) preparation of sodium alginate grafted tea polyphenol antibacterial dressing
Adding gelatin and glycerol into the tea polyphenol grafted sodium alginate complex solution prepared in the step (1), degassing and casting at 35-40 ℃, and then adding calcium chloride metal solution to solidify for 2-3 hours to obtain a formed gel film; then dissolving chitosan in an organic acid solvent at 40-42 ℃ to form a chitosan acid solution, wherein the pH value is 4.0-4.5; and finally, soaking the gel film in chitosan acid liquor for 8-10 hours to form the solid sodium alginate grafted tea polyphenol antibacterial dressing.
2. The preparation method of the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the mass-to-volume ratio of the sodium alginate to the acetic acid solution in step (1) is 1 g: (50-70 mL); wherein the mass fraction of the acetic acid solution is 5-8%.
3. The preparation method of the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the mass ratio of sodium alginate to tea polyphenol in step (1) is 1 g: 1.7-2.5 g.
4. The preparation method of the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the mass ratio of the tea polyphenol-grafted sodium alginate complex, gelatin and glycerol in step (2) is (4-6) g: (3-5) g: 1g of the total weight of the composition.
5. The method for preparing the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the degassing casting process in the step (2) is as follows: degassing at vacuum degree of not less than-0.090 MPa; and pouring the formed slurry onto a 20 cm-by-20 cm dry and clean glass dish for casting to form a film, wherein the thickness of the slurry is controlled to be 0.05-0.1 cm.
6. The preparation method of the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the mass fraction of the calcium chloride metal solution in the step (2) is 2-3%.
7. The preparation method of the sodium alginate-grafted tea polyphenol antibacterial dressing as claimed in claim 1, wherein the mass-to-volume ratio of the chitosan to the organic acid in the step (2) is 1 g: 20-40 mL; wherein the organic acid can be one of acetic acid, citric acid and malic acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116949031A (en) * | 2023-09-20 | 2023-10-27 | 南京信息工程大学 | Application of straw efficient decomposition microbial inoculum in straw degradation |
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|---|---|---|---|---|
| WO2012085881A1 (en) * | 2010-12-23 | 2012-06-28 | Danisco A/S | Microbicidal composition |
| CN103446621A (en) * | 2013-05-07 | 2013-12-18 | 哈尔滨工程大学 | Nano-silver-containing sodium alginate based antibacterial medical dressing and preparation method thereof |
| CN103463667A (en) * | 2013-05-07 | 2013-12-25 | 哈尔滨工程大学 | Preparation method of nanosilver-carried calcium alginate antibiotic medical dressing |
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| CN116949031A (en) * | 2023-09-20 | 2023-10-27 | 南京信息工程大学 | Application of straw efficient decomposition microbial inoculum in straw degradation |
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