CN103463184A - Traditional Chinese medicine microecology preparation for treating diarrhea caused by dysbacteriosis - Google Patents
Traditional Chinese medicine microecology preparation for treating diarrhea caused by dysbacteriosis Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine microecology preparation for treating diarrhea caused by dysbacteriosis. The preparation comprises the effective components: 20%-50% of a dosage of ultramicro qiweibaizhu powder, 0.2-0.5*10<10> debaryomyces hansenii, that is to say, 20%-50% of the dosage of the ultramicro qiweibaizhu powder and 0.2-0.5*10<10> debaryomyces hansenii are mixed uniformly and adsorbed, and can reach the curative effect of 1 dosage of a qiweibaizhu powder; each dosage of the qiweibaizhu powder comprises 6 g of ginseng, 6 g of radix aucklandiae, 10 g of white poria, 10 g of roasted rhizoma atractylodis macrocephalae, 10 g of agastache leaf, 10 g of radix puerariae and 3 g of glycyrrhiza uralensis, and each dosage of the ultramicro qiweibaizhu powder is prepared from 1 dosage of the qiweibaizhu powder by a traditional Chinese medicine ultramicro technology. The compatibility of the ultramicro qiweibaizhu powder with debaryomyces hansenii has important significance for development and utilization of novel traditional Chinese medicine preparations.
Description
Technical field
The invention belongs to Chinese medicine microbial ecological agent field, is exactly a kind of medicament of suffering from diarrhoea that Chinese medicine compound and yeast compatibility are prevented and treated due to dysbacteriosis.
Background technology
It is in recent years, antibiotic that to be widely used the phenomenon that causes dysbacteriosis even to produce drug resistance day by day serious.The seven flavor Rhizoma Atractylodis Macrocephalaes shed from money second key to Therapeutics of Children's Diseases, cure mainly diarrhea due to hypofunction of the spleen, Radix Ginseng, Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI in side, Poria, Herba Pogostemonis, the Radix Aucklandiae and middle removing dampness are regulated the flow of vital energy, Radix Puerariae the lucid yang sending up gas, and its cube is rigorous, compatibility is precise and appropriate, and Quan Fangrong benefit, fortune, ascending, descending are integrated, tonify without causing stagnation.Clinical practice and modern pharmacological research prove, seven the flavor Rhizoma Atractylodis Macrocephalaes are loose that the multiple intestinal tract diseases such as Infant deferred diarrhea, fungal enteritis, ulcerative colitis, damp abundance due to splenic asthenia type diarrhoea are had to good therapeutic effect.Li Chunying has carried out the loose research that adds flavor treatment antibiotic-associated diarrhea of the seven flavor Rhizoma Atractylodis Macrocephalaes, the loose flavor that adds of the proof seven flavor Rhizoma Atractylodis Macrocephalae has remarkable therapeutical effect to antibiotic-associated diarrhea, Tan Zhoujin etc. have confirmed that the loose compound recipe of the seven flavor Rhizoma Atractylodis Macrocephalaes has obvious regulating and controlling effect to enteric microorganism and enzymatic activity, and loose 50% dosage of the ultra micro seven flavor Rhizoma Atractylodis Macrocephalae can reach the curative effect of traditional decoction pieces full dose, can the saving Chinese medicaments material.
Micro-Ecological regulation microbial inoculum settles down, breeds in intestinal, to promote and to maintain microecological balance in intestinal, adjust the physiological equilibrium, pathogen in intestinal is directly exerted an influence or by intestinal microecology structure or immune adjustment are exerted an influence indirectly, thereby can get rid of pathogenic bacterium and conditioned pathogen invasion and attack.At present, a plurality of bacterial strains of Lactobacillus, Bifidobacterium, spore mushroom and Saccharomycodes have been applied to clinical as microecologic regulator.Yeast can produce a large amount of vitamin B group and aminoacid, both can help digest, and promotes the absorption of nutrient substance, stimulates the growth of probiotics, can be again animal protein is provided.Yeast can be participated in the formation of biological barrier directly, and the antagonism of performance to pathogenic bacterium and conditioned pathogen promotes the dominant population growth, adjusts microecological balance.The intestinal yeast also can carry out the micro-Ecological regulation in intestinal by its metabolite, thus the immunity of enhancing body and premunition.
Chinese medicine and microbial ecological agent coupling have become a current important research field.Cui's method newly utilizes Baitouweng Tang and Gancao Ejiao Tang and microbial ecological agent golden bifid coupling treatment to obtain effect preferably to the invalid Pediatric bacterial dysentery of antibiotic, there are some researches show that Chinese medicine and microbial ecological agent coupling can significantly improve the SOD in serum of broilers, the activity of GSH-Px, reduce the content of MDA in serum, obviously improve the oxidation resistance of body.The applicant has studied the loose effect with Hansenula yeast bacterium therapeutic alliance mice dysbacteria diarrhea of the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes, also inquired into the loose best compatibility with the Hansenula yeast bacterium of relation between its curative effect and intestinal microbial population and the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes, the present invention adopts the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes to fall apart and Hansenula yeast bacterium therapeutic alliance dysbacteria diarrhea mice first, and has obtained comparatively ideal effect.
Summary of the invention
Purpose of the present invention aims to provide a kind of Chinese medicine microbial ecological agent, and diarrhoea due to dysbacteriosis is had to good therapeutic effect, and saving Chinese medicaments material effectively, economizes on resources, and further reduces antibiotic use.
A kind of Chinese medicine microbial ecological agent of suffering from diarrhoea for the treatment of due to dysbacteriosis is prepared from by following mode:
1) the seven flavor Rhizoma Atractylodis Macrocephalaes with Radix Ginseng 6g, Radix Aucklandiae 6g, Poria 10g, Rhizoma Atractylodis Macrocephalae (parched) 10g, Radix agastaches 10g, Radix Puerariae 10g, 1 dosage of Radix Glycyrrhizae 3g composition are loose, get the loose 20-50% of the seven flavor Rhizoma Atractylodis Macrocephalaes of 1 dosage, then micronizing becomes the powder of size 0.01-500 μ m, obtains ultramicro decoction piece;
2) by the inferior Dbaly yeast bacterium activation of the Chinese, be inoculated into the potato sucrose fluid medium, after 28 ℃ 150 rev/mins shaking table shaken cultivation 36h, the centrifugal 10min of 3000rpm, abandon supernatant, and precipitation repeated washing 1-2 time, obtain the yeast thalline;
The loose decoction pieces of the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes of the 20%-50% dosage 3) prepared by step 1) adds step 2) the yeast thalline 0.2-0.5 that obtains * 10
10individual, mix homogeneously absorption, obtain solid Chinese medicine microbial ecological agent.
The Chinese medicine microbial ecological agent of suffering from diarrhoea due to described treatment dysbacteriosis, preferentially select the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes of 25% dosage to fall apart+0.25 * 10
10individual yeast mixing and absorption prepares the Chinese medicine microbial ecological agent.
The applicant is carrying out state natural sciences fund (81173214; 81070295) in the process of research, find that Radix Ginseng and Radix Glycyrrhizae have produced inhibitory action to the intestinal yeast, wherein the minimum inhibitory concentration of Radix Ginseng is 0.7936g/ml, and the minimum inhibitory concentration of Radix Glycyrrhizae is 0.3949g/ml; Saccharomycetic growth has produced facilitation to intestinal for Poria, the Rhizoma Atractylodis Macrocephalae, Radix Puerariae, and Radix Puerariae is best to the facilitation effect of intestinal Yeast Growth, and Poria takes second place; And the Radix Aucklandiae and Herba Pogostemonis do not show obvious inhibition or facilitation to saccharomycetic being grown in this research of intestinal.Under low concentration, to intestinal, saccharomycetic growth has produced facilitation to the loose compound recipe of the seven flavor Rhizoma Atractylodis Macrocephalaes, and, by comparative analysis, the loose ultramicro decoction piece of the seven flavor Rhizoma Atractylodis Macrocephalaes is better than traditional decoction pieces to the facilitation effect of intestinal Yeast Growth.In this way, we have studied, and the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes are loose treats the problem of dysbacteria diarrhea with the yeast compatibility, and has obtained content of the present invention.The present invention adopts the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes to fall apart and Hansenula yeast bacterium therapeutic alliance dysbacteria diarrhea mice first, and has obtained comparatively ideal effect.
The specific embodiment
Following examples are intended to illustrate the present invention rather than limitation of the invention further.
Embodiment 1
The clean level of 1 animal kunming mice, male and female half and half, weight 20 ± 2g, reaching the laboratory animal company limited by Hunan Si Laike scape provides.
Loose each amounts of components of the 2 medicine 1 dosage seven flavor Rhizoma Atractylodis Macrocephalae is: Radix Ginseng 6g, Radix Aucklandiae 6g, Poria 10g, Rhizoma Atractylodis Macrocephalae (parched) 10g, Radix agastaches 10g, Radix Puerariae 10g, Radix Glycyrrhizae 3g; While making the loose ultramicro decoction piece of the seven flavor Rhizoma Atractylodis Macrocephalaes of 50% dosage, 25% dosage, 12.5% dosage, every kind of 50% amount, 25% amount or 12.5% amount that component is all only got each plant demand namely, mixing becomes size 0.01-500 μ m powder by micronizing, make ultramicro decoction piece, save backup in 4 ℃ of refrigerators.
The inferior Dbaly yeast bacterium of the Chinese: by the inferior Dbaly yeast bacterium activation of the Chinese, be inoculated into the potato sucrose fluid medium, after 28 ℃ 150 rev/mins shaking table shaken cultivation 36h, the centrifugal 10min of 3000rpm, abandon supernatant, precipitation repeated washing (washing with sterilized water) 1-2 time (each centrifugal 10min of 3000rpm), obtain the yeast thalline.In 4 ℃ of refrigerators, save backup.
After raising 4d, 3 animal grouping mice adaptabilities are divided at random 7 groups, i.e. normal group, model group, the loose group of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalae, the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.125 * 10
10individual yeast group, the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10individual yeast group, 12.5% dosage ultra micro decoction+0.5 * 10
10individual yeast group, 10
10individual yeast group.Every group 6, male and female half and half, sub-cage rearing.Feedstuff and place are provided by Hunan University of Traditional Chinese Medicine's zoopery center.
4 modeling methods are made into the antibiotic mixed liquor that concentration is 62.5g/L (6 gentamycins (2mL/ props up)+3 cephalo (0.25g/ grain) is mixed with mixed liquor) by Cefradine Capsules and gentamicin injection liquid with physiological saline solution, in 4 ℃ of refrigerators, save backup.It is only inferior that normal group gives physiological saline solution 0.35mL/() gavage, all the other each groups adopt antibiotic mixed liquor 0.35mL/ (only inferior) gavage.Every day 2 times, continuous 5d, feces become " rare wet " be the modeling success.
After 5 medications and dosage modeling success, every day, gastric infusion was 2 times, continuously 3d.Normal group and model group give physiological saline solution, and all the other each groups are by the loose traditional decoction pieces full dose clinical medicine dose equivalence administration of the seven flavor Rhizoma Atractylodis Macrocephalaes.
6 effect analysis method modeling successes are rear, treatment takes respectively Mouse Weight after finishing, the diet activity of observation animal, mental status, feces dilution factor, death condition etc., and calculate the body weight change rate, computing formula is as follows: body weight change rate (%) after treatment=(body weight after final body weight-modeling)/final body weight * 100.And after treatment finishes, the mice of execution is put on superclean bench immediately, aseptic collection is respectively organized the intestinal contents of jejunum to ileal segment, collects mouse intestinal content on the same group and is mixed by sterile working.Take a certain amount of intestinal contents and put into the aseptic water bottle that bead is housed by organizing respectively sterile working, mixed bacterium method counting, antibacterial, coliform are counted bacterium colony cultivate 24h in 37 ℃ of incubators after, fungus is counted bacterium colony cultivate 48h in 30 ℃ of incubators after, lactobacillus, bacillus bifidus are counted bacterium colony cultivate 48h in 37 ℃ of anaerobic culture boxes after, each dilution factor is done 3 repetitions, asks its meansigma methods and calculates the contained bacterium number of every gram intestinal contents.With DPS v7.05 software data processing, each gained continuous data that divides into groups adopts mean+SD
mean, between two groups, relatively, with the t check, there is statistical significance P<0.05 to mean.
The 7 ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes fall apart and the curative effect of yeast coupling to the mice dysbacteria diarrhea
In Table 1.Before and after the mice treatment, stool colour, water content, viscosity etc. all change, and treat the 1st, and mice fresh excreta color is still all black, and more moistening; Treat the 2nd, all stool in mice treat the 1st day rarer, may be the delayed response of antibiotic diarrhea inducing, treatment group mice fresh excreta color starts flavescence, the model group stool in mice is still black; Treat the loose group of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalae, the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.125 * 10 the 3rd
10individual yeast group, the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10individual yeast group stool in mice is become a cadre, and color is thin out, and model group, 12.5% dosage ultra micro decoction+0.5 * 10
10individual yeast group, 10
10individual yeast group stool in mice is still rarer wet, and color is darker.
Table 1 ultra micro seven is distinguished the flavor of, and the Rhizoma Atractylodis Macrocephalaes fall apart and the effect of yeast Synergistic treatment mice dysbacteria diarrhea
Annotate :+meaning the feces drying ,-expression loose and watery stool is wet.
The 8 ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes fall apart and the collaborative impact on the dysbacteria diarrhea Mouse Weight of yeast
As shown in Table 2, after modeling, administration group and model group weight of mice rate are lower than normal group, after gavage finishes, administration group and model group body weight gain rate be higher than normal group, but and the more equal not statistically significant of Normal group (P > 0.05), in this and therapeutic process, each administration group diarrhea of mouse symptom is constantly alleviated, and the model group mice is also in continuous self-recovery, and each mental status of organizing mice takes a turn for the better, food-intake increases relevant, and the seven flavor Rhizoma Atractylodis Macrocephalaes are loose not remarkable on the Mouse Weight impact with the yeast coupling.
The table 2 ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes are loose works in coordination with the impact on the dysbacteria diarrhea Mouse Weight with yeast
The 9 ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes fall apart and the collaborative impact on the microorganism of dysbacteria diarrhea mouse intestinal of yeast
As shown in Table 3, after treatment finishes, model group coliform count, fungus number are apparently higher than normal group (P<0.01 or P<0.05), total number of bacteria, bacillus bifidus number and lactic acid bacteria number are significantly lower than normal group (P<0.01 or P<0.05), and the loose group of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalae, the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes fall apart+0.125 * 10
10individual yeast group, the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10individual yeast group, the 12.5% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.5 * 10
10individual yeast group and 10
10the coliform count of individual yeast group all significantly reduces (P<0.05) than model group, wherein the and 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10 loose through the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes
10coliform count and normal group after the treatment of individual yeast are the most approaching, but difference remarkable (P>0.05).Each treatment group bacterial population, bacillus bifidus number, lactic acid bacteria number and fungus number all increase (P<0.01 or P<0.05) to some extent than model group, wherein the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10the approaching the most of the bacterial population of individual yeast group and normal group (P>0.05); The 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.125 * 10
10individual yeast group and the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10the bacillus bifidus number of individual yeast group and lactic acid bacteria number and the loose group of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes approach, and difference is (P>0.05) not significantly.The bacillus bifidus number of yeast group, lactic acid bacteria number and fungus number are all higher than other groups (P<0.01 or P<0.05).Therefore, the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10individual yeast is the most approaching to the regulating and controlling effect of dysbacteria diarrhea mouse intestinal microorganism and the loose curative effect of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes, and 10
10individual yeast has remarkable facilitation (p<0.01 or p<0.05) to intestinal beneficial bacterium bacillus bifidus and lactobacillus propagation.
The table 3 ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes are loose works in coordination with the impact on the microorganism of dysbacteria diarrhea mouse intestinal with yeast
Annotate: with the normal group group relatively, a:P<0.01, A:P<0.05; With model group relatively, b:P<0.01, B P<0.05 is with loose the group relatively of the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes, c:P<0.01, C:P<0.05; With the 50% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.125 * 10
10individual yeast group compares, d:P<0.01, D:P<0.05; With the 25% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10
10individual yeast group is e:P<0.01 relatively, E:P<0.05; With the 12.5% dosage ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.5 * 10
10individual yeast group compares: f:P<0.01, F:P<0.05
The present invention confirms, the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10 of 25% dosage
10individual yeast mixing and absorption is best compatibility, the effect of its treatment dysbacteriosis diarrhea of mouse is suitable with loose 50% dosage of the ultra micro seven flavor Rhizoma Atractylodis Macrocephalae, can reach the curative effect of the loose traditional decoction pieces full dose of the seven flavor Rhizoma Atractylodis Macrocephalaes, for exploitation and the production of clinical treatment and new drug provides experimental basis.
Claims (2)
1. treat due to dysbacteriosis the Chinese medicine microbial ecological agent of suffering from diarrhoea for one kind, it is characterized in that, be prepared from by following mode:
1) the seven flavor Rhizoma Atractylodis Macrocephalaes with Radix Ginseng 6g, Radix Aucklandiae 6g, Poria 10g, Rhizoma Atractylodis Macrocephalae (parched) 10g, Radix agastaches 10g, Radix Puerariae 10g, 1 dosage of Radix Glycyrrhizae 3g composition are loose, get the loose 20-50% of the seven flavor Rhizoma Atractylodis Macrocephalaes of 1 dosage, then micronizing becomes the powder of size 0.01-500 μ m, obtains ultramicro decoction piece;
2) by the inferior Dbaly yeast bacterium activation of the Chinese, be inoculated into the potato sucrose fluid medium, after 28 ℃ 150 rev/mins shaking table shaken cultivation 36h, the centrifugal 10min of 3000rpm, abandon supernatant, and precipitation repeated washing 1-2 time, obtain the yeast thalline;
The loose decoction pieces of the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes of the 20%-50% dosage 3) prepared by step 1) adds step 2) the yeast thalline 0.2-0.5 that obtains * 10
10individual, mix homogeneously absorption, obtain solid Chinese medicine microbial ecological agent.
2. the Chinese medicine microbial ecological agent of suffering from diarrhoea due to treatment dysbacteriosis according to claim 1, is characterized in that, selects the ultra micro seven flavor Rhizoma Atractylodis Macrocephalaes loose+0.25 * 10 of 25% dosage
10individual yeast mixing and absorption prepares the Chinese medicine microbial ecological agent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403947A (en) * | 2018-04-17 | 2018-08-17 | 南宁学院 | A kind of Chinese medicine composition and preparation method thereof for treating bamboo rat diarrhea |
| CN108686172A (en) * | 2018-08-16 | 2018-10-23 | 深圳简体美健康信息咨询有限公司 | Composition, preparation method and applications method for improving fat metabolism equilibrium |
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| Title |
|---|
| 戴斌彬: "微生物中草药复合益生元的筛选及对AA肉鸡生长性能肠道微生物区系及血清抗氧化性能影响的研究", 《中国优秀硕士学位论文全文数据库》 * |
| 谭周进: "超微七味白术散对肠道微生物及酶活性的影响", 《生态学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403947A (en) * | 2018-04-17 | 2018-08-17 | 南宁学院 | A kind of Chinese medicine composition and preparation method thereof for treating bamboo rat diarrhea |
| CN108686172A (en) * | 2018-08-16 | 2018-10-23 | 深圳简体美健康信息咨询有限公司 | Composition, preparation method and applications method for improving fat metabolism equilibrium |
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