CN103461233A - Stress resistance measuring device and use method thereof - Google Patents
Stress resistance measuring device and use method thereof Download PDFInfo
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- CN103461233A CN103461233A CN2013103447760A CN201310344776A CN103461233A CN 103461233 A CN103461233 A CN 103461233A CN 2013103447760 A CN2013103447760 A CN 2013103447760A CN 201310344776 A CN201310344776 A CN 201310344776A CN 103461233 A CN103461233 A CN 103461233A
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- outer container
- inner core
- test
- resistance determinator
- stress resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a stress resistance measuring device and a use method thereof, which aims at measuring the stress resistance under adverse conditions by effectively adjusting and controlling the living environment of Chinese mitten crabs according to the test requirements. The stress resistance measuring device comprises an inner cylinder and an outer box, wherein the inner cylinder is arranged in the outer box, the outer box is provided with a top cover, a water inlet and a water outlet, the inner cylinder is provided with a support framework, the support frame is attached with separating nets, and culture layers and culture chambers are formed through the separating nets. The stress resistance measuring device has the advantages that the inner cylinder adopts the integral design, and the structure is simple; a crab is placed in each culture chamber, the mutual interference is avoided, and the smooth testing is ensured; fifteen crabs can be placed in the stress resistance measuring device, so the test requirement is met; the outer box adopts the transparent design, so the testing condition can be observed at any time; and the five culture layers are arranged in the inner cylinder, so the land occupation area for testing is saved; during water injection, a drug reagent can be fed into the water according to the testing requirement; and a plurality of testing concentrations can be simultaneously measured.
Description
[technical field]
The present invention relates to resistance determination techniques technical field, specifically, is a kind of for Eriocheir sinensia resistance determinator and using method thereof.
[background technology]
Eriocheir sinensia on taxonomy in Arthropoda, Crustachia, Decapoda, Grapsidae, Eriocheir, its growth is fast, individual large, delicious meat, have higher economic worth.The cultivation of Eriocheir sinensia mainly is divided into indoor culture and pond culture, and this breeding way can only provide breeding environment generally, measures the resistance of Eriocheir sinensia under different condition also inadequate.
Chinese patent literature CN:201210391039.1 discloses a kind of small-sized enclosure Eriocheir sinensis technology.Chinese patent literature CN:201010608511.3 discloses a kind of high flux acute toxic test method of fish early life stage.But resistance testing arrangement and using method thereof about Eriocheir sinensia yet there are no report.
At present about Eriocheir sinensia of the present invention, at the resistance determinator of various extreme environments, yet there are no report.
[summary of the invention]
The objective of the invention is for deficiency of the prior art, the device that provides a kind of Eriocheir sinensia resistance to measure, in order to study the resistance of Eriocheir sinensia under the varying environment condition.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
A kind of resistance determinator, comprise inner core and outer container, and described inner core is placed in outer container, it is characterized in that, described inner core is provided with support frame, and on described support frame, with separate mesh, described outer container is provided with top cover, water inlet and delivery port.Described support frame is cylindrical.
Described outer container is cylindrical, and the bottom surface radius is 15cm, and height is 65cm.
The upper and lower two ends of described support frame are provided with handle and support, and there is a main shaft at center.
Described handle, support and main shaft, its material is stainless steel.
Described separate mesh is divided into five cultivation layers by inner core.
Described cultivation layer is divided into three culturing chambers by separate mesh.
Described culturing chamber outer wall respectively is provided with a wicket.
Described separate mesh and wicket adopt the stainless (steel) wire material to make.
The lucite that described outer container and top cover material are processed for anti-ultraviolet.
Described water inlet pipe is positioned at the outer container sidewall, apart from outer container bottom surface 5cm.
Described outlet pipe is positioned on top cover, apart from top cover edge 5cm.
Compared with prior art, the invention has the advantages that:
1, inner core adopts integrated design, compact conformation;
2, respectively put a test crab in each receptacle, do not have each other interference, guarantee test carries out smoothly;
3, a resistance determinator can be put 15 crabs altogether, can meet the demand of test data statistics;
4, outer container adopts transparent design, viewing test situation at any time;
5, inner core is provided with five cultivation layers and saves the test floor space;
6, can add according to test requirements document medicine and reagent during water filling in water;
7, can be simultaneously by a plurality of devices mensuration of carrying out a plurality of experimental concentration in parallel.
[accompanying drawing explanation]
Accompanying drawing 1 is the overall schematic of resistance determinator inner core of the present invention and outer container.
Accompanying drawing 2 is support frame structure figure of resistance determinator of the present invention.
Accompanying drawing 3 is separate mesh structure charts of resistance determinator of the present invention.
The Reference numeral and the part that in accompanying drawing, relate to are as follows:
1. inner core 2. outer containers
11. support frame 12. separate mesh
21. oral siphon 22. outlet pipes
23. top cover 111. main shafts
112. support 113. handles
121. cultivation layer 122. culturing chamber
123. wicket
[embodiment]
Below in conjunction with accompanying drawing, embodiment provided by the invention is elaborated.
Embodiment 1:
Refer to Fig. 1, accompanying drawing 1 is the overall schematic of resistance determinator of the present invention.Described resistance determinator is provided with inner core 1 and outer container 2, and described inner core 1 is placed in outer container 2, and described outer container 2 is provided with top cover 23, and described top cover 23 is cylindrical, and described outer container 2 is connected to water inlet pipe 21 and outlet pipe 22.Described oral siphon 21 is placed on described outer container 2 walls, and described outlet pipe 22 is placed on described top cover 23.
Refer to Fig. 2, accompanying drawing 2 is structure charts of the support frame 11 of inner core in resistance determinator of the present invention.Described inner core 1 inside is provided with support frame 11, and there is a main shaft 111 at described support frame 11 centers.Described support frame 11 tops are handle 113.Described support frame 11 bottoms are support 112.
Refer to Fig. 3, accompanying drawing 3 is separate mesh 12 structure charts of resistance determinator of the present invention.Described separate mesh 12 is divided into five cultivation layers 121 by inner core (not marking in the drawings), Figure 3 shows that one of them described cultivation layer 121.Described cultivation layer 121 is divided into three culturing chambers 122 by described separate mesh 12, and described culturing chamber 122 outer walls respectively have a wicket 123.
It should be noted that, described outer container 2 and top cover 23 materials are lucite; Described support frame 11, main shaft 111, the material of handle 113 and support 112 is stainless steel; Described separate mesh 12 and wicket 123 materials are stainless (steel) wire.Outer container described in apparatus of the present invention 2 is cylindrical, and the bottom surface radius is 15cm, and height is 65cm, and the support frame 11 in described inner core 1 and outer container 2 are all cylindrical, and support frame 11 bottom surface radiuses are 10cm, high 60cm.Described top cover 23 bottom surface radiuses are 16cm, and height is 5cm, than the large 0.8-1.8cm of outer container radius all in tolerance interval.Resistance determinator described in apparatus of the present invention also can adopt other sizes and shape, as the shape of the support frame 11 in the shape of outer container 2 and inner core 1 is chosen as rectangle or triangle; The optional 10cm-25cm of outer container 2 bottom surface radius, the high optional 50cm-80cm of outer container, the optional 5cm-20cm of support frame 11 bottom surface radius in inner core 1, high 45cm-75cm; Oral siphon 21 apart from described outer container 2 bottom surface 5cm(3cm-8cm all can), outlet pipe 22 apart from described top cover 23 edge 5cm(3cm-8cm all can); The high 5cm(3cm-8cm of described support frame 11 top handle 113 all can), the high 5cm(3cm-8cm of described support frame 11 bottom bracket 112 all can); The quantity of cultivation layer 121, culturing chamber 122 is depending on the bodily form specification of test crab.Concrete numerical value too much do not mention in the present embodiment, but the modification of the shape based on apparatus of the present invention, material, size, layout is not limited to above showed embodiment all within protection scope of the present invention.
During test, the test crab is put into to the culturing chamber 122 of inner core 1, inner core 1 is placed in to outer container 2, cover top cover 23.Water is passed into to outer container 2 and inner core 1 by oral siphon 21, and unnecessary water can be discharged from outlet pipe 22, makes the water yield in inner core 1 remain certain.By controlling water level and the flow velocity of the whole device of oral siphon 21 flow-control, flow control is advisable at the 0.3-0.6 meter per second.Can add by test requirements document medicine and reagent before entering water in water.During as a plurality of experimental concentration of need, can adopt the use in parallel of a plurality of devices simultaneously.
Embodiment 2: to the toxotest of ammonia nitrogen
In the average specification of Eriocheir sinensia for larger, (be less than 5% in the wide basically identical situation of body, if the i.e. test crab of the wide 8cm of selective body, body wide regions of other test crabs should be controlled in 7.6cm-8.4cm), use the tolerance of apparatus of the present invention test Eriocheir sinensia to ammonia nitrogen.Concrete steps: 1, will test the cell in the crab charging apparatus, every chamber is placed one, assembles complete device; 2, add in blank group of test (adding without ammonia nitrogen) water 5 hours, treat that its enteron aisle is emptying and adapt to new environment; The water of each concentration gradient that 3, will prepare is in advance progressively changed former blank water by water inlet pipe, with instrument monitoring water outlet ammonia nitrogen concentration, whether reaches test requirements document; 4, whole duration of test keeps miniflow water (2cm/s) to maintain the stable of enough dissolved oxygens and ammonia nitrogen concentration; 5, observe the death condition (or coordinating video camera work) of river crab.Benefit: its test operation and traditional toxicity test device are compared, and save 80% floor space; Save a large amount of time, because can regulate at any time the concentration of ammonia nitrogen; Save a large amount of labor costs, by 3 people, be reduced to 1 people (as used video camera, more can save laboratory technician's time at the scene); Be compared to traditional error 15%, device experiment data repeatability is high, and error is in 7% left and right.Can carry out many group tests simultaneously.Test period can be passed through transparent window, holds flexibly.Transparent window can be observed the behavioral activity under its different ammonia nitrogen concentrations at any time, as tentatively judging from now on poisoning sign foundation.
Embodiment 2: to the toxotest of hypoxemia
In the long average specification of Eriocheir sinensia body, be 5 ± 0.1 mm, (be less than 5% in the wide basically identical situation of body, if the i.e. test crab of the wide 8cm of selective body, body wide regions of other test crabs should be controlled in 7.6cm-8.4cm), use apparatus of the present invention to test its hypoxemia tolerance.Concrete operation step can be with reference to step described in embodiment 1.Benefit: its test operation and traditional experimental rig are compared, and can effectively avoid the mutual fierce fight of river crab; Save test 80% floor space; Simple to operate because installing, saved for 50% test operation time; Reduce 10% than the conventional method error.Can once carry out 5, the even more test of polyoxy concentration simultaneously.Simultaneously, transparent window can be observed the action behavior under its hypoxemia at any time.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the inventive method; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection scope of the present invention.
Claims (10)
1. a resistance determinator, comprise inner core and outer container, and described inner core is placed in outer container, and described outer container is provided with top cover, water inlet and delivery port, and described inner core is provided with support frame, it is characterized in that, has separate mesh on described support frame.
2. resistance determinator according to claim 1, is characterized in that, the upper and lower two ends of described support frame are provided with handle and support, and there is a main shaft at center.
3. resistance determinator according to claim 1, is characterized in that, described separate mesh is divided into five cultivation layers by inner core.
4. resistance determinator according to claim 3, is characterized in that, described cultivation layer is divided into three culturing chambers by separate mesh.
5. resistance determinator according to claim 4, is characterized in that, described culturing chamber outer wall respectively is provided with a wicket.
6. resistance determinator according to claim 1, is characterized in that, the lucite that described outer container and top cover material are processed for anti-ultraviolet.
7. the using method of a resistance determinator, resistance determinator as claimed in claim 1, is characterized in that, comprises the following steps:
A. by the test crab of packing in inner core, put into outer container, cover top cover,
B. test water is passed into to outer container and inner core by oral siphon.
8. the using method of resistance determinator according to claim 7, is characterized in that, in described step B, can control water level and the flow velocity of whole device by controlling the oral siphon flow.
9. according to the using method of the described resistance determinator of claim 7 or 8, it is characterized in that, in described step B, described test water can be added medicine and reagent by test requirements document before injecting oral siphon.
10. the using method of resistance determinator according to claim 7, is characterized in that, described test is during as a plurality of experimental concentration of need, can adopt that a plurality of devices are in parallel to be used simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103447760A CN103461233A (en) | 2013-08-07 | 2013-08-07 | Stress resistance measuring device and use method thereof |
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| CN2013103447760A CN103461233A (en) | 2013-08-07 | 2013-08-07 | Stress resistance measuring device and use method thereof |
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| CN103461233A true CN103461233A (en) | 2013-12-25 |
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| CN2013103447760A Pending CN103461233A (en) | 2013-08-07 | 2013-08-07 | Stress resistance measuring device and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488790A (en) * | 2014-12-24 | 2015-04-08 | 江苏省淡水水产研究所 | Device for observing molt and growth of river crabs and application thereof |
Citations (9)
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|---|---|---|---|---|
| US3870019A (en) * | 1971-02-10 | 1975-03-11 | Douglas Mcnicol | Oyster culture basket |
| GB1399933A (en) * | 1972-08-08 | 1975-07-02 | George R | Holding assemblage for off-bottom culture of oysters and analogous shellfish |
| US4377987A (en) * | 1981-10-30 | 1983-03-29 | Satre Alf R | System for growing oysters |
| US5515813A (en) * | 1994-05-09 | 1996-05-14 | Wilkerson; Douglas D. | Aquatic cultivator |
| CN1993042A (en) * | 2004-09-27 | 2007-07-04 | 阿夸股份有限公司 | A basket for shellfish farming |
| CN101576554A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Dynamic running water type toxicity test device of fishes |
| CN101576555A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Static temperature controlled toxicity test device of fishes |
| CN101946741A (en) * | 2010-07-26 | 2011-01-19 | 浙江海洋学院 | Swimming crab culturing device |
| CN103053458A (en) * | 2013-01-16 | 2013-04-24 | 潘少波 | Ecological breeding method of scylla serrata |
-
2013
- 2013-08-07 CN CN2013103447760A patent/CN103461233A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3870019A (en) * | 1971-02-10 | 1975-03-11 | Douglas Mcnicol | Oyster culture basket |
| GB1399933A (en) * | 1972-08-08 | 1975-07-02 | George R | Holding assemblage for off-bottom culture of oysters and analogous shellfish |
| US4377987A (en) * | 1981-10-30 | 1983-03-29 | Satre Alf R | System for growing oysters |
| US5515813A (en) * | 1994-05-09 | 1996-05-14 | Wilkerson; Douglas D. | Aquatic cultivator |
| CN1993042A (en) * | 2004-09-27 | 2007-07-04 | 阿夸股份有限公司 | A basket for shellfish farming |
| CN101576554A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Dynamic running water type toxicity test device of fishes |
| CN101576555A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Static temperature controlled toxicity test device of fishes |
| CN101946741A (en) * | 2010-07-26 | 2011-01-19 | 浙江海洋学院 | Swimming crab culturing device |
| CN103053458A (en) * | 2013-01-16 | 2013-04-24 | 潘少波 | Ecological breeding method of scylla serrata |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488790A (en) * | 2014-12-24 | 2015-04-08 | 江苏省淡水水产研究所 | Device for observing molt and growth of river crabs and application thereof |
| CN104488790B (en) * | 2014-12-24 | 2017-01-11 | 江苏省淡水水产研究所 | Device for observing molt and growth of river crabs and application thereof |
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Application publication date: 20131225 |