CN105557608B - A kind of aquatic animal toxicological experiment system and application - Google Patents

A kind of aquatic animal toxicological experiment system and application Download PDF

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Publication number
CN105557608B
CN105557608B CN201510973722.XA CN201510973722A CN105557608B CN 105557608 B CN105557608 B CN 105557608B CN 201510973722 A CN201510973722 A CN 201510973722A CN 105557608 B CN105557608 B CN 105557608B
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blowdown
cultivation unit
split tunnel
aquatic animal
unit
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CN105557608A (en
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吴玲玲
黄清辉
陈玲
郜洪文
李倩
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Tongji University
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Tongji University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/045Filters for aquaria
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of aquatic animal toxicological experiment system and applications, the experimental group formed including at least one by cultivation unit and filtering inflation unit, cultivation unit is made of multiple breeding troughs and a blowdown split tunnel, the lower part of each breeding trough is connected with blowdown split tunnel, filtering inflation unit includes water pumping mechanism, inflation mechanism and the cyclic water tank being connected to blowdown split tunnel, filter medium is equipped with inside cyclic water tank, the sewage for cultivating unit discharge passes through filter media, after inflation mechanism inflation, cultivation unit is recycled by the cultivation unit water inlet pipe connecting with water pumping mechanism;The system can be applied in terms of organic pollutant breeds aquatic animal toxicological experiment, physical chemical factor difference to the experiment of aquatic animal physiological effect and aquatic animal.Compared with prior art, the present invention can high quality realize that the liquid in the processing of more parallel groups is balanced and automatic pollutant removal, while reducing cost of labor and human interference.

Description

A kind of aquatic animal toxicological experiment system and application
Technical field
The present invention relates to aquatic animal exposure experimental provision and applications, more particularly, to a kind of aquatic animal toxicological experiment System and application.
Background technique
1. being had at present using the method that zebra fish carries out organic matter toxicological test is that partial volume device hydrostatic is raised, parallel group is arranged (same concentrations group) and control group, residual bait, excrement is sucked out in timing, and replaces testing liquid.2. using the flowing water group for dividing parallel group It attaches together and sets raising, every parallel group independently filters residual bait, excrement, and replaces testing liquid.But the above method is 1. in test process Soil pick-up is time-consuming: when test, Partial Species organic matter volatilizees from solution in test process or is adsorbed in excrement, residual bait, container Wall etc., each container difference soil pick-up of parallel group, the difference for replacing solution amount respectively etc., will lead to molten in each parallel group container Large variation occurs for liquid concentration, causes test solution concentration in each container of parallel group to form difference, serious reduce repeats test effect Fruit.2. pollutant is easy to volatilize from solution or be adsorbed in excrement, residual bait, chamber wall etc., put down the above method during the test Each container of row group absorbs residual bait excrement respectively, and each container of parallel group cannot carry out solution equilibrium very well, equally cause parallel group each Solution concentration fluctuates in container, reduces and repeats test effect, and residual bait excrement heavy workload is sucked out.If volatile contaminant Toxicity test, 1. 2. pollutant volatilization be easy to cause indoor pollution to method with method in experimentation, threatens experimenter's body Health.
Chinese patent CN201821759U discloses the aquatic organism cultivating system that a kind of drip water-feeding type is used for toxicological experiment, Including bracket, supporting layer, cultivation flume, return flume, purification tank for liquid waste, filtering cloth jar and water-supply-pipe with multilayer support layer And control valve;It is equipped with water storage box in cradle top, the dribble-feed water inlet pipe of cultivation flume is made up of hydraulic pipeline and control valve Road;Unidirectional cultivation water system is constituted further through drainpipe outside cultivation flume and breeding trough and purification tank for liquid waste;In the support Layer is equipped with the water bath of water temperature in controllable breeding trough, and the cultivation flume is placed in water bath;In addition, described Water bath by the water bath water inlet, the water bath overflow plate that are arranged on water bath, and with the matched water bath of water bath Backflash, water bath return pipe, filtering cloth jar constitute temperature control circulator bath system;It is furnished with charge valve in the cultivation flume top. Water system in the patent is connection water-supply-pipe and control valve group by being connected on each cultivation flume at from top to bottom Water system, the solution concentration of toxicological experiment is then consistent, and cannot achieve parallel exposure in groups.And this system is without dirt Object collector unit, the cleaning that not can guarantee exposed environments are stablized, and the breeding of aqueous bio is unfavorable for.
Chinese patent CN102830202A discloses a kind of closed static aquatic organism toxicity experiment device, including transparent Experiment container, oxygen supply container, faecal collecting device, the transparent experiment container upper surface are openable and closable cover board, It is equipped with oxygen supply container in the transparent experiment container, is that funnel-form connects in the middle part of the transparent experiment container lower surface Component, the funnel-form connecting component lower end connect faecal collecting device.Although the experimental provision can the row of collection at any time Object is let out, bottom center is funnel-shaped, and lower end connects faecal collecting device, but needs manually opened discharge/collection, and exposes body It is that solution will also be flowed out with discharge/collection process, such as exposure experiment continues, then needs again to return to solution after being separated by solid-liquid separation In exposure container.
Therefore, the prior art cannot achieve aquatic animal toxicological test device automatic pollutant removal and parallel exposure etc. in groups is crucial Problem.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide one kind being capable of high quality reality Now the liquid equilibrium in more parallel group processing and automatic pollutant removal, while cost of labor and human interference are reduced, it can effectively reduce Pollutant, which volatilizees, leads to the aquatic animal toxicological experiment system of indoor pollution, and it is real which can be used for aquatic animal toxicity It tests, physical chemical factor difference is to the experiment of aquatic animal physiological effect and aquatic animal breeding cultivation etc..
The purpose of the present invention can be achieved through the following technical solutions:
A kind of aquatic animal toxicological experiment system is formed including at least one by cultivation unit and filtering inflation unit Experimental group,
The cultivation unit is made of multiple breeding troughs and a blowdown split tunnel, the lower part of each breeding trough with row Dirty split tunnel is connected,
The filtering inflation unit includes water pumping mechanism, inflation mechanism and the recirculated water being connected to blowdown split tunnel Case, cyclic water tank inside are equipped with filter medium, and the sewage of cultivation unit discharge is by filter media, inflation mechanism inflation Afterwards, cultivation unit is recycled by the cultivation unit water inlet pipe connecting with water pumping mechanism.
The front side of each breeding trough is arranged in multiple breeding trough horizontal alignments arrangement, blowdown split tunnel.
The blowdown split tunnel is by being arranged in the blowdown split tunnel partition and cultivation unit foreboard that cultivate in unit Composition forms the breeding trough of multiple horizontal alignment arrangements by partition between blowdown split tunnel partition and cultivation unit back plate, Gap is formed on the bottom of the blowdown split tunnel partition and cultivation unit so that the lower part of each breeding trough separated with blowdown it is logical Road is connected.
The bottom plate of the box for breeding is tilted to the side of blowdown split tunnel.
Cyclic water tank is divided into filtering module and the mould that draws water by the vertical partition of setting in the cyclic water tank, the partition Block, the bottom of partition and the bottom of box form gap, and gap location is equipped with filter medium, and the filtering module separates logical with blowdown Road connection, the water pumping mechanism and gas supply mechanism are arranged in pumping module side.
The filter medium includes blocky or film-form filtering material, and the water pumping mechanism is water pump, air feeder Structure is gas charging stone, and gas charging stone is connected with oxygen charging pump.
The cultivation unit water inlet pipe is located at the top of breeding trough, and water outlet is equipped with valve;The cultivation upper box part Equipped with the sliding door for feeding bait;The cyclic water tank top is equipped with the sliding door for fluid infusion.
The filtering module is connect by inverted U-shaped siphon pipe with one end of blowdown split tunnel, system work When filtering module side liquid level be lower than blowdown split tunnel liquid level.
The cyclic water tank is mounted on the right side of box for breeding, and the bottom plate of cyclic water tank is lower than the bottom of cultivation unit Portion.
The system includes multiple experimental groups formed by cultivation unit and filtering inflation unit, and each experimental group is in vertical direction On be sequentially arranged.
Toxicological experiment, physics of a kind of aquatic animal toxicological experiment system in organic pollutant to aquatic animal Application of the chemokines difference to the physiological effect experiment of aquatic animal and the breeding cultivation aspect of aquatic animal.
The physical chemical factor includes the physical and chemical factors such as pharmacology, temperature, salinity, pH value or nutrition.
When experiment, the number of the number of experimental group and the breeding trough (horizontal group) of each experimental group is set as needed, it will Experimental liquid is packed into the system, opens water pumping mechanism and inflation mechanism, makes experimental liquid in cultivation unit and filtering inflation unit Between recycle, adjust cultivation unit water inlet pipe correspond to each breeding trough water outlet valve, keep cultivation unit liquid level height Degree be greater than filtering inflation unit, with guarantee inverted u-shaped siphon pipe can work well, system stablize after, by water body to be investigated Biology is put into each breeding trough, corresponding sliding door is opened when needing to feed bait and liquid feeding, usually sliding door is closed with maximum journey Degree reduces indoor pollution caused by pollutant volatilization.
Compared with prior art, the invention has the following advantages that
(1) present invention can high quality realizes that pollutant concentration is equal in necessary more parallel group processing during scientific experiment Weighing apparatus, can be improved the science of experiment and examines the repeatability of experimental result, quality of experiments is greatly improved.Each experimental group packet The parallel group that multiple breeding troughs are constituted is included, and the blowdown split tunnel partition bottom of each breeding trough is separated with box for breeding bottom Gap so that the lower part of each breeding trough is connected with blowdown split tunnel, while making the amount ratio of the liquid of each breeding trough It is more consistent, the sewage in each breeding trough is discharged with water flow by blowdown split tunnel, dirt enters filtering inflation with water flow Then unit is re-circulated into each breeding trough through filtering inflation, can guarantee to the full extent in the same experimental group in this way Each breeding trough in liquid various physical chemical factors consistency.
(2) it using inverted U-shaped siphon pipe connection blowdown split tunnel and filtering inflation unit, realizes in cultivation unit The discharge of sewage, sewage require no the power-equipments such as pump, are not in the damage caused by power device of the dirt in sewage, It runs relatively reliable;Matched, the bottom plate of cyclic water tank is lower than the bottom plate of box for breeding, to facilitate realization siphon.
(3) bottom of each breeding trough of cultivation unit of the invention is tilted to blowdown split tunnel, and residual bait and excrement etc. are dirty Object is easier to concentrate the discharge for facilitating dirt to lateral access by circulating water flow.
(4) disturbance that residual bait excrement etc. is sucked out to experimental animal can be greatly reduced in the present invention, and blowdown process is automatic Carry out, do not need human intervention, reduce cost of labor and human interference, fluid infusion be also by filtering inflation unit, so as to To solve the problems, such as that fluid infusion is skimble-scamble.
(5) present invention especially adapts to organic pollutant to aquatic animal toxicological test, cultivates unit and filtering inflation unit It is all made of cabinet, and is provided with sliding door, it is only necessary to open in bait throwing in material and fluid infusion, be waved so as to effectively reduce pollutant Indoor pollution caused by sending out.
(6) present invention can also apply to the physical and chemical factors differences such as pharmacology, temperature, salinity, pH value, nutrition to aquatic animal Physiological effect test, it can also be used to aquatic animal breeding cultivation.It is suitble to the experiment of zebra fish, medaka, Bellamya Aeruginosa Reeve, if Large-scale aquatic animal can suitably be increased in size.
Detailed description of the invention
Fig. 1 is schematic view of the front view of the invention;
The position Fig. 2 side structure schematic view of the invention;
In figure, 1 is cultivation unit, and 101 be knob, and 102 be the first breeding trough, and 103 be the second breeding trough, and 104 be third Breeding trough, 105 be breeding trough partition, and 106 be blowdown split tunnel partition, and 107 be cultivation unit foreboard, and 108 be cultivation unit Back plate, 109 be cultivation unit baseboard, and 110 be cultivation unit sliding door, and 111 be cultivation unit liquid level, and 112 be blowdown split tunnel, 2 be filtering inflation unit, and 201 be cyclic water tank, and 202 be blocky filtering material, and 203 be cyclic water tank partition, and 204 be water pump, 205 be gas charging stone, and 206 pull for cyclic water tank, and 3 be inverted u-shaped siphon pipe, and 4 is single for cultivation for cultivation unit water inlet pipe, 401 First water inlet pipe water outlet, 5 be the first experimental group, and 6 be the second experimental group, and 7 be third experimental group, and 8 be the 4th experimental group, and 9 be the Five experimental groups.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
A kind of aquatic animal toxicological experiment system, as shown in Fig. 1~2, including arrange vertically five by cultivation unit 1 The experimental group formed with filtering inflation unit 2, the present embodiment (are suitble to minitype aquatic animal by high 2.8m × long 2m × wide 0.5m Such as zebra fish) glass aquarium in be arranged glass partition be separated into five cultivation units, pass through one group high 40cm × length Five glass boxs of 30m × wide 0.5m form five filtering inflation units, are separately mounted to the right side of cultivation unit being attached thereto Side.
Cultivation unit 1 therein is made of multiple breeding troughs and a blowdown split tunnel 112, by cultivating unit back plate 108, two parallel breeding troughs of the blowdown split tunnel partition 106 parallel with cultivation unit back plate 108, connection said two devices Partition 105, cultivation unit left side plate and cultivation unit baseboard 109 form the first breeding trough 102, the second breeding trough 103 and the The breeding trough of three breeding troughs 104 totally three horizontal alignments arrangement, constitutes the parallel group in an experimental group, blowdown split tunnel every The gap that width is 1cm is equipped between plate 106 and cultivation unit foreboard 107, which forms blowdown split tunnel 112, blowdown The bottom of split tunnel partition 106 is not contacted with cultivation unit baseboard 109, is spaced the distance of 0.3~0.5cm therebetween, from And each breeding trough lower part is connected to blowdown split tunnel, and cultivate unit baseboard 109 and incline to 112 direction of blowdown split tunnel Tiltedly.
Filtering inflation unit 2 therein includes water pumping mechanism, inflation mechanism cyclic water tank 201, setting in cyclic water tank 201 Vertical cyclic water tank partition 203, the partition divide cyclic water tank 201 for filtering module and pumping module, cyclic water tank partition 203 bottom and the bottom of cyclic water tank 201 are separated with gap, and gap location is equipped with blocky filtering material 202 and is used as filter medium, should The filtering module of cyclic water tank 201 is connected to blowdown split tunnel 112 by inverted u-shaped siphon pipe 3, inverted u-shaped siphon pipe 3 one End is located at blowdown split tunnel right end close to bottom, and dirt is facilitated to enter inverted u-shaped siphon pipe 3 with water flow, real in order to be easier Existing siphon, the bottom of cyclic water tank is lower than the cultivation about 10cm of unit baseboard 109, when work, keeps cultivation unit liquid level 111 high Liquid level in 201 filtering module side of cyclic water tank, water pump 204 are arranged as water pumping mechanism, gas charging stone 205 as inflation mechanism Pumping module side, gas charging stone 205 are connected with oxygen charging pump, the sewage that cultivation unit 1 is discharged by the blocky filtering of filtering material 202, After the gas charging stone 205 connecting with oxygen charging pump is inflated, cultivation is recycled by the cultivation unit water inlet pipe 4 connecting with water pump 204 Unit 1, cultivation unit water inlet pipe 4 are located at the top of each breeding trough, pipe equipped with cultivation unit corresponding with each breeding trough into Water pipe water outlet 401, cultivation unit water inlet pipe water outlet 401 are equipped with valve, and cultivation 1 top of unit is equipped with for feeding bait Unit sliding door 110 is cultivated, cultivation unit sliding door 110 is equipped with knob 101, and it is convenient that cultivation unit sliding door 110 is pushed and pulled, it controls Make its opening and closing.201 top of cyclic water tank is equipped with the cyclic water tank sliding door 206 for fluid infusion.
The aquatic animal toxicological experiment system of the present embodiment can be used for organic pollutant to aquatic animal toxicological experiment And physical chemical factor difference, to aquatic animal physiological effect experiment etc., zebra fish, which has, is easy raising, in vitro fertilization, embryo The advantages that tire is transparent, genetics technology is mature has become one of important model animal of ecotoxicology research in recent years, selects its work To test aqueous bio used.When experiment, the first experimental group 5, the second experimental group 6, third experimental group 7 is set as needed, 4th experimental group 8 and the 5th experimental group 9 totally five experimental groups, each experimental group is by the first breeding trough 102, the second breeding trough 103 and third breeding trough 104 totally three breeding troughs form horizontal group, experimental liquid is packed into the system, open water pumping mechanism and Inflation mechanism recycles experimental liquid between cultivation unit and filtering inflation unit, and it is corresponding every to adjust cultivation unit water inlet pipe 4 The valve of the water outlet of a breeding trough keeps the height of the liquid level 111 of cultivation unit to be greater than the liquid level of filtering inflation unit, to protect Demonstrate,prove inverted u-shaped siphon pipe can work well, system stablize after, aqueous bio to be investigated is put into each breeding trough, need Opening corresponding sliding door when feeding bait and liquid feeding, usually sliding door is closed utmostly to reduce room caused by pollutant volatilization Interior pollution is changed liquid or fluid infusion and is completed via filtering inflation unit.
Embodiment 2
The present embodiment is substantially the same manner as Example 1, the difference is that the present embodiment passes through in blowdown split tunnel 112 1 The hole being connected to is bored to realize that sewage is filled to filtering with the corresponding site of the filtering module side of cyclic water tank 201 in the lower part at end Gas unit 2 flows.
Embodiment 3
The device of the present embodiment is substantially the same manner as Example 1, the difference is that the present embodiment is numerous applied to aquatic animal It brings up and grows aspect.Human cost and human interference can be greatly reduced, exclude dirt in time, facilitate the breeding of aqueous bio.
Embodiment 4
The present embodiment is substantially the same manner as Example 1, the difference is that, the filter medium of the present embodiment is the mistake of film-form Filter material matter is arranged in the gap location of the bottom of cyclic water tank partition 203 and cyclic water tank 201, plays the role of filter sewage.

Claims (4)

1. a kind of aquatic animal toxicological experiment system, which is characterized in that inflated including at least one by cultivation unit and filtering The experimental group that unit is formed,
The cultivation unit is made of multiple breeding troughs and a blowdown split tunnel, and the lower part of each breeding trough is divided with blowdown It is connected from channel,
The filtering inflation unit includes water pumping mechanism, inflation mechanism and the cyclic water tank being connected to blowdown split tunnel, is followed Filter medium is equipped with inside ring water tank, the sewage of cultivation unit discharge passes through after filter media, inflation mechanism inflation The cultivation unit water inlet pipe connecting with water pumping mechanism is recycled into cultivation unit;
The front side of each breeding trough is arranged in multiple breeding trough horizontal alignments arrangement, blowdown split tunnel;
The blowdown split tunnel is made of the blowdown split tunnel partition being arranged in cultivation unit with cultivation unit foreboard, The breeding trough of multiple horizontal alignment arrangements is formed between blowdown split tunnel partition and cultivation unit back plate by partition, it is described Gap is formed on the bottom of blowdown split tunnel partition and cultivation unit, so that the lower part of each breeding trough is connected with blowdown split tunnel It is logical;
The bottom of the cultivation unit is tilted to the side of blowdown split tunnel;
Cyclic water tank is divided into filtering module and pumping module by the vertical partition of setting, the partition in the cyclic water tank, every The bottom of plate and the bottom of box form gap, and gap location is equipped with filter medium, and the filtering module and blowdown split tunnel connect Logical, the water pumping mechanism and gas supply mechanism are arranged in pumping module side;
The filtering module is connect by inverted U-shaped siphon pipe with one end of blowdown split tunnel;
The cyclic water tank is mounted on the right side of box for breeding, and the bottom plate of cyclic water tank is lower than the bottom of cultivation unit.
2. a kind of aquatic animal toxicological experiment system according to claim 1, which is characterized in that the filter medium Including blocky or film-form filtering material, the water pumping mechanism is water pump, and the gas supply mechanism is gas charging stone, gas charging stone It is connected with oxygen charging pump.
3. a kind of aquatic animal toxicological experiment system stated according to claim 1, which is characterized in that the cultivation unit into Water pipe is located at the top of breeding trough, and cultivation unit water inlet pipe is equipped with water outlet corresponding with each breeding trough, and water outlet is equipped with valve; The top of the cultivation unit is equipped with the sliding door for feeding bait;The cyclic water tank top is equipped with the drawing for fluid infusion Door.
4. a kind of aquatic animal toxicological experiment system as described in claims 1 to 3 is any is in organic pollutant to aquatic dynamic The toxicological experiment of object, physical chemical factor difference cultivate aspect to the physiological effect experiment and the breeding of aquatic animal of aquatic animal Application.
CN201510973722.XA 2015-12-21 2015-12-21 A kind of aquatic animal toxicological experiment system and application Active CN105557608B (en)

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CN110074037B (en) * 2019-05-22 2021-04-06 河海大学 Device for researching toxicological effect of pollutants on fishes
CN110208517A (en) * 2019-07-03 2019-09-06 上海海洋大学 A kind of Temperature Control Type Ecology chronic toxicity test device
CN110231449A (en) * 2019-07-03 2019-09-13 上海海洋大学 A kind of circulation Ecology acute toxicity test system
CN111323553A (en) * 2020-03-13 2020-06-23 浙江省海洋水产研究所 A pond of breeding fish for toxicological experiment
CN111838052B (en) * 2020-06-01 2022-10-28 北京爱生科技发展有限公司 Miniature-mode biological running water type experiment cup and liquid medicine cylinder for toxicology experiment

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CN102177867A (en) * 2011-02-24 2011-09-14 西南大学 Efficient and economical indoor circulating aquaculture system
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