CN103436443A - Integrated zero-leakage gas positive-negative pressure compatible microorganism isolation operation cabinet - Google Patents
Integrated zero-leakage gas positive-negative pressure compatible microorganism isolation operation cabinet Download PDFInfo
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- CN103436443A CN103436443A CN201310399337XA CN201310399337A CN103436443A CN 103436443 A CN103436443 A CN 103436443A CN 201310399337X A CN201310399337X A CN 201310399337XA CN 201310399337 A CN201310399337 A CN 201310399337A CN 103436443 A CN103436443 A CN 103436443A
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Abstract
The invention provides an integrated zero-leakage gas positive-negative pressure compatible microorganism isolation operation cabinet which comprises a positive pressure cabin with a positive pressure fan and a sterile filter at the top and a glass sliding door, wherein the positive pressure cabin is surrounded by a glass front partition plate and a glass side partition plate to form a negative pressure cabin; two sealed isolation sleeves formed by overlapping and interacting an outer partition soft plate and an inner partition soft plate are arranged on the glass front partition plate; on a working table of the negative pressure cabin, a circle of negative-pressure air grid ports are communicated with a negative pressure filtering cabin with a gas filtering material; and the negative pressure fan is arranged in a fan chamber communicated on the lower side of the negative pressure filtering cabin to extract the filtered waste gas to an exhaust connector to be discharged. The zero-leakage gas positive-negative pressure compatible microorganism isolation operation cabinet provided by the invention has the beneficial effects that the device can ensure that the cells and bacteria cultured in a microorganism experiment process are free from the contamination and interference of other bacteria in a sterile positive pressure environment, and harmful microorganisms and gases inside can be isolated and shielded by the negative pressure cabin so as to prevent leakage and consequent environmental pollution and occupational injury; and the device is widely applied to the scientific research fields such as environmental protection, medical scientific research, military security and the like.
Description
Technical field
The present invention relates to the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet.
Background technology
In recent years in environment protection, military security, bio-science, medical and health, animals and plants is learned, carry out in a large number bacterium in breathing and toxicologic study, the experiment of cell cultures and drug intervention effect, at cell, in microbial culture and scientific experiment, need one can guarantee that cell survives normally, the cleaning of growth and experiment, dustless, aseptic gas positive pressure environment, bacterium meanwhile, cell itself has certain infection may, particularly carrying out some obnoxious flavour aerosols needs again some poisonous and hazardous materials aerosolized for tests such as cell exposures, be easy to diffusion operating environment of pollution, even the operator is damaged, the aerosol that for example cultivation of respiratory infectious microorganism and effect of drugs are intervened exposes experiment, not only need in aseptic positive pressure environment, cultivate good respiratory infectious microorganism and can not have other bacteriums to disturb but also must under the negative pressure isolation, strictly control this kind of microorganism and leak and cause environmental pollution and employment injuries for one exactly, this just needs the gnotobasis that an inside is malleation to cultivate and experimental cell, the single microorganisms such as bacterium, the all gas that can collect discharge in positive ballasting of outside encirclement, negative pressure cab without some leakages, and have the convenient operation experiments at any time of seal isolation oversleeve, independent malleation aseptic technique cabinet and independent negative pressure isolated operation cabinet have been arranged in the industry at present or the positive/negative-pressure compatible operations cabinet do not contained mutually be installed together has side by side been arranged, but also do not have to surround with the isolation negative pressure cab microorganism isolated operation cabinet that aseptic positive ballasting guarantees zero leakage gas specially, guarantee cultured cells in the Experiment on Microbiology process, thereby bacterium etc. in aseptic positive pressure environment, be not subject to interference that other living contaminantses cause can rely on again the isolation negative pressure shield harmful microorganism or obnoxious flavour for environment and operator produce injury satisfactorily complete this type of scientific research that this type of test restricted deeply and development, therefore designing and developing the gnotobasis that an inside is malleation cultivates and the single microorganisms such as experimental cell, bacterium, the all gas that can collect discharge in positive ballasting of outside encirclement, without the negative pressure cab of some leakages, and the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet device that has a convenient operation experiments at any time of seal isolation oversleeve just becomes current urgent needs.
Summary of the invention
For solving the problem existed in above-mentioned technology, the purpose of this invention is to provide the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet.
For achieving the above object, technical scheme of the present invention is to provide the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet, comprise that a top is equipped with the positive ballasting of positive pressure fan and sterilizing filter, the gnotobasis that is used for producing internal positive pressure is cultivated and experimental cell, the single microorganisms such as bacterium, inner uv steriliser and ozonateur are being installed on the rear wall of positive ballasting can be aligned at any time ballasting inside and carry out disinfection to guarantee gnotobasis, there is glass slide moving up and down its front portion, keep apart be enclosed in malleation out of my cabin section can collect all gas of discharge in positive ballasting, the negative pressure cab formed by dividing plate before the glass without some leakages and glass side dividing plate, and before the glass in negative pressure cab right on dividing plate parallel be equipped with two by outside every soft board with interiorly every the mutual juxtaposition of soft board, form the convenient operation experiments at any time of seal isolation oversleeve, have a circle negative pressure air grid mouth on the work top of negative pressure cab and be communicated with the inner negative pressure filtration cabin that gas filtering material is housed, negative-pressure air fan in following seal fan cabin, negative pressure filtration cabin discharges the Abgasabsaugung after filtering to effluxing interface.
The invention has the beneficial effects as follows: the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet device is provided, utilize the gnotobasis that an inside is malleation to cultivate and experimental cell, the single microorganisms such as bacterium, the all gas that can collect discharge in positive ballasting of outside encirclement, negative pressure cab without some leakages, and have the convenient device of operation experiments at any time of seal isolation oversleeve, both solved cultured cells in assurance Experiment on Microbiology process, the interference that bacterium etc. are not caused by other living contaminantses in aseptic positive pressure environment has solved again and can rely on the isolation negative pressure to shield harmful microorganism or obnoxious flavour produces the scientific research difficult problem of injury for environment and operator, filled up technological gap in this respect, in environment protection, military security, bio-science, medical and health, animals and plants is learned, widespread use in breathing and toxicologic study field.
The accompanying drawing explanation
Accompanying drawing one and figure bis-are the schematic diagram of structure of the present invention.
In figure:
1, dividing plate 4, negative pressure air grid mouth before tackleblock 2, glass slide 3, glass
5, work top 6, efflux interface 7, glass side dividing plate 8, positive pressure fan
9, sterilizing filter 10, sealed strip 11, negative pressure cab 12, manual pressure handle
13, seal isolation oversleeve 14, outward every soft board 15, negative-pressure air fan 16, negative pressure filtration cabin
17, positive ballasting 18, counterweight post 19, sliding rope 20, uv steriliser
21, interior every soft board 22, ozonateur 23, blower fan cabin.
Embodiment
In conjunction with figure mono-, figure bis-and embodiment, the present invention is illustrated.
As shown in figure mono-and figure bis-, the invention provides the compatible microorganism isolated operation of a kind of integrated zero leakage gas positive-negative pressure cabinet, comprise a positive ballasting 17 that produces the internal positive pressure gnotobasis, positive ballasting 17 tops are equipped with positive pressure fan 8 and sterilizing filter 9, positive ballasting 17 is by corrosion-resistant, smooth surface, the material of sterilization easy to clean forms, inner strict seal manufacture, top directly over positive ballasting 17 is equipped with efficient sterilizing filter 9 and the positive pressure fan 8 that can filter bacterium and dust impurity, extraneous air is blowed in positive ballasting 17 through filtering the aseptic uncontaminated air formed, form from top to bottom, air-flow from inside to outside, positive ballasting 17 bottom surfaces are work tops 5, positive ballasting 17 left side walls and the right wall are smooth seamless, guarantee cabin body sealing, right in positive ballasting 17 only has a glass slide 2, round-the-clock air-flow from inside to outside can guarantee in positive ballasting 17 not have outside bacterium and enter in positive ballasting 17, inner uv steriliser 20 and ozonateur 22 are being installed on the rear wall of positive ballasting 17 can be aligned at any time ballasting 17 inside and carry out disinfection to guarantee gnotobasis, there is glass slide 2 moving up and down positive ballasting 17 front portions, glass slide 2 is plane valve that a transparent glass is made and can easy on and off moves, glass slide 2 is equipped with the position that manual pressure handle 12 handled easily personnel improve or reduce glass slide 2 below, the sealed strip 10 that isolated gas is installed below of manual pressure handle 12, glass slide 2 also is equipped with the doorframe surrounding sealed strip 10 that completely cuts off gas, in the time of can guaranteeing 2 slip of glass slide, air seal is not revealed, glass slide 2 tops are connecting a tackleblock 1, tackleblock 1 is restricted and 19 is connected a counterweight post 18 by cunning, the steady state of glass slide 2 in the time of can keeping operating at any time, make the nonvoluntary slip of glass slide 2, glass slide 2 can keep apart be enclosed in positive ballasting 17 outsides by glass before the negative pressure cab 11 that forms of dividing plate 3 and glass side dividing plate 7, negative pressure cab 11 be one from front portion, three directions in the left side and the right surround the cavity of the sealing of positive ballasting 17, dividing plate 3 before its right on is equipped with the glass that transparent material makes, on the left and the right also are separately installed with the glass side dividing plate 7 that transparent material is made, negative pressure cab 11 is not revealed with the positive ballasting 17 whole sealings in connection portion, before the glass in negative pressure cab 11 right ons on dividing plate 3 parallel be equipped with two by outside every soft board 14 with interiorly every the mutual juxtaposition of soft board 21, form the convenient operation experiments at any time of seal isolation oversleeve 13, seal isolation oversleeve 13 is seal frames, framework be with outward a circle can with dividing plate before glass 3 on the rubber guard ring that is sealed and matched of perforate, inner ring is the oversleeve mouth that can the handled easily human arm puts in release, the oversleeve mouth respectively is equipped with up and down one and accounts for the outer every soft board 14 and interior every the mutual juxtaposition sealing of soft board 21 installation of most areas, airtight at ordinary times can gas leakage, when stretch into operation during arm outside every soft board 14 and interior every gap of the softened formation of soft board 21, although some gas leakage but can be not a lot, simultaneously can be to outward leakage because so the interior suction function of negative pressure cab 11 can only have extraneous air to the interior inflow of negative pressure cab 11, have a circle negative pressure air grid mouth 4 and be communicated with the negative pressure filtration cabin 16 that gas filtering material is equipped with in the following inside of work top 5 on the work top 5 of negative pressure cab 11, negative pressure filtration cabin 16 is enclosed housings of being made by corrosion resistant material, the work top 5 that is fixedly installed to negative pressure cab 11 is following, be communicated to negative pressure cab 11 by negative pressure air grid mouth 4, its inside has been full of gac etc. and has been conducive to the material that gas absorption is filtered, from all gas of positive ballasting 17 and 13 inflows of seal isolation oversleeve, all from negative pressure air grid mouth 4, gush, the following blower fan cabin 23 that connecting of the enclosed housing in negative pressure filtration cabin 16, negative-pressure air fan 15 in the blower fan cabin 23 of sealing can produce certain negative pressure-pumping ability while working, by in negative pressure cab 11 the waste exhaust gases suction filtration after be sent to and efflux interface 6 discharges.
embodiment:
During use, at first the interface 6 use pipelines connections that efflux of the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure cabinet are led to outside laboratory, unlatching is arranged on the negative-pressure air fan 15 in seal fan cabin 23, have a circle negative pressure air grid mouth 4 and be communicated with the negative pressure filtration cabin 16 that gas filtering material is equipped with in the following inside of work top 5 on the work top 5 of negative pressure cab 11, it is following that negative pressure filtration cabin 16 is fixedly installed to the work top 5 of negative pressure cab 11, its inside has been full of gac etc. and has been conducive to the material that gas absorption is filtered, the following blower fan cabin 23 that connecting of the enclosed housing in negative pressure filtration cabin 16, negative-pressure air fan 15 in seal fan cabin 23 can produce certain negative pressure-pumping ability while working, by in negative pressure cab 11 the waste exhaust gases suction filtration after be sent to and efflux interface 6 discharges, by hand stretch into seal isolation oversleeve 13 will be outward every soft board 14 with interiorly every soft board 21, strut and form a gap, although some gas leakage but can be not a lot, simultaneously can be to outward leakage because so the interior suction function of negative pressure cab 11 can only have extraneous air to the interior inflow of negative pressure cab 11, pull the glass slide 2 of 17 front portions, malleation cabin downwards, connect uv steriliser 20 and ozonateur 22 power supplys installed on the rear wall of positive ballasting 17, align ballasting 17 sterilizing that carries out disinfection, guarantee that positive ballasting 17 inside are gnotobasiss, unlatching subsequently is arranged on the efficient sterilizing filter that can filter bacterium and dust impurity 9 and the positive pressure fan 8 directly over positive ballasting 17, extraneous air is blowed in positive ballasting 17 through filtering the aseptic uncontaminated air formed, form from top to bottom, air-flow from inside to outside, then glass slide 2 just can move up, to shift to an earlier date ready experiment material and cell, the article such as bacterium are put on the work top 5 in the positive ballasting 17 in the glass slide 2 upwardly opened by seal isolation oversleeve 13, carry out the series of experiments operation, can press at any time the position that the following manual pressure handle of glass slide 2 12 reduces glass slides 2 when do not operated, the sealed strip 10 that isolated gas is installed below of manual pressure handle 12, when the sealed strip 10 that glass slide 2 and doorframe surrounding also are equipped with isolated gas can guarantee that glass slide 2 slides, air seal is not revealed, glass slide 2 tops are connecting a tackleblock 1 and counterweight post 18, the nonvoluntary slip of steady state in the time of can keeping operating at any time, when handle is extracted out from seal isolation oversleeve 13, the oversleeve mouth respectively is equipped with up and down one and accounts for the outer every soft of most areas, 14 and interior can gas leakage every the sealing of the mutual juxtaposition of soft board 21, because negative pressure cab 11 be one from front portion, three directions in the left side and the right surround the cavity of the sealing of positive ballasting 17, dividing plate 3 before its right on is equipped with the glass that transparent material makes, on the left and the right also are separately installed with the glass side dividing plate 7 that transparent material is made, glass slide 2 be can see through and the interior all experimental implementation processes of positive ballasting 17 and article situation clearly observed, can stretch into experiment material and the cell that arm has taken out successively from seal isolation oversleeve 13 after completing all tests, the article such as bacterium, close successively positive pressure fan 8, press the position that manual pressure handle 12 that glass slide 2 is equipped with below reduces glass slides 2 downwards, finally close negative-pressure air fan 15 and complete all experiments were.
Claims (6)
1. the compatible microorganism isolated operation of an integrated zero leakage gas positive-negative pressure cabinet, it is characterized in that: comprise that a top is equipped with the positive ballasting (17) of positive pressure fan (8) and sterilizing filter (9), be used for producing the internal positive pressure gnotobasis, inner uv steriliser (20) and ozonateur (22) are being installed on the rear wall of positive ballasting (17) can be aligned at any time ballasting (17) inside and carry out disinfection to guarantee gnotobasis, there is glass slide moving up and down (2) positive ballasting (17) front portion, glass slide (2) is kept apart and is enclosed in the outside negative pressure cab (11) formed by dividing plate before glass (3) and glass side dividing plate (7) of positive ballasting (17), and before the glass in negative pressure cab (11) right on dividing plate (3) upper parallel be equipped with two by outside every soft board (14) and interior every soft board (21) seal isolation oversleeve (13) convenience that juxtaposition forms mutually operation experiments at any time, have a circle negative pressure air grid mouth (4) on the work top (5) of negative pressure cab (11), be communicated with the following inside of work top (5) the negative pressure filtration cabin (16) of gas filtering material is being housed, the following interior negative-pressure air fan (15) in seal fan cabin (23) in negative pressure filtration cabin (16) discharges the Abgasabsaugung after filtering to effluxing interface (6).
2. the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure according to claim 1 cabinet, it is characterized in that: described positive ballasting (17) is by corrosion-resistant, smooth surface, the material of sterilization easy to clean forms, inner strict seal manufacture, top directly over positive ballasting (17) is equipped with efficient sterilizing filter (9) and the positive pressure fan (8) that can filter bacterium and dust impurity, extraneous air is blowed in positive ballasting (17) through filtering the aseptic uncontaminated air formed, form from top to bottom, air-flow from inside to outside, positive ballasting (17) bottom surface is work top (5), positive ballasting (17) left side wall and the right wall are smooth seamless, guarantee cabin body sealing, only have a glass slide (2) in right on, round-the-clock air-flow from inside to outside can guarantee in positive ballasting (17) that not having outside bacterium enters in positive ballasting (17).
3. the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure according to claim 1 cabinet, it is characterized in that: described glass slide (2) is the plane valve that a transparent glass is made and can easy on and off moves, glass slide (2) is equipped with the position that manual pressure handle (12) handled easily personnel improve or reduce glass slide (2) below, the sealed strip (10) that isolated gas is installed below of manual pressure handle (2), when the sealed strip (10) that glass slide (2) and doorframe surrounding also are equipped with isolated gas can guarantee that glass slide (2) slides, air seal is not revealed, glass slide (2) top is connecting a tackleblock (1), tackleblock (1) by cunning restrict (19) connect a counterweight post (18), the steady state of glass slide (2) in the time of can keeping operating at any time, make the nonvoluntary slip of glass slide (2).
4. the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure according to claim 1 cabinet, it is characterized in that: described negative pressure cab (11) be one from front portion, three directions in the left side and the right surround the cavity of the sealing of positive ballasting (17), dividing plate (3) before its right on is equipped with the glass that transparent material makes, on the left and the right also are separately installed with the glass side dividing plate (7) that transparent material is made, negative pressure cab (11) is not revealed with the positive whole sealings in ballasting (17) connection portion, have a circle negative pressure air grid mouth (4) on the work top (5) of negative pressure cab (11), from all gas of positive ballasting (17) and seal isolation oversleeve (13) inflow, all from negative pressure air grid mouth (4), gush.
5. the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure according to claim 1 cabinet, it is characterized in that: described seal isolation oversleeve (13) is a seal frame, framework is with the rubber guard ring that a circle can be sealed and matched with the upper perforate of dividing plate before glass (3) outward, inner ring is the oversleeve mouth that can the handled easily human arm puts in release, the oversleeve mouth respectively is equipped with up and down one and accounts for the outer every soft board (14) and interior every soft board (21) juxtaposition sealing installation mutually of most areas, airtight at ordinary times can gas leakage, when stretch into operation during arm outside every soft board (14) and interior every gap of the softened formation of soft board (21), although some gas leakage but can be not a lot, simultaneously can be to outward leakage so interior suction function can only have extraneous air to interior inflow of negative pressure cab (11) due to negative pressure cab (11).
6. the compatible microorganism isolated operation of integrated zero leakage gas positive-negative pressure according to claim 1 cabinet, it is characterized in that: described negative pressure filtration cabin (16) is an enclosed housing of being made by corrosion resistant material, the work top (5) that is fixedly installed to negative pressure cab (11) is following, be communicated to negative pressure cab (11) by negative pressure air grid mouth (4), its inside has been full of gac etc. and has been conducive to the material that gas absorption is filtered, the following blower fan cabin (23) that connecting at enclosed housing, negative-pressure air fan (15) in the blower fan cabin (23) of sealing can produce certain negative pressure-pumping ability while working, efflux interface (6) discharge by being sent to after the waste exhaust gases suction filtration in negative pressure cab (11).
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105858578A (en) * | 2016-05-26 | 2016-08-17 | 深圳市廖氏原生物医学科技有限公司 | Specimen stationary liquid taking instrument |
| CN107126907A (en) * | 2016-02-26 | 2017-09-05 | 南京中硼联康医疗科技有限公司 | Pharmaceutical synthesis device |
| CN107412818A (en) * | 2015-09-11 | 2017-12-01 | 洛阳佳嘉乐农产品开发股份有限公司 | A kind of efficient, the intensive ozone workplace of planting edible mushroom sterilizing |
| CN107523496A (en) * | 2017-10-18 | 2017-12-29 | 安徽理工大学 | A kind of installation for fermenting of comprehensive environmental experiment |
| CN107714358A (en) * | 2017-10-30 | 2018-02-23 | 潍坊潍医医院 | A kind of helium oxygen mixing automatic feeding system |
| CN114768914A (en) * | 2022-05-12 | 2022-07-22 | 江苏中慧元通生物科技股份有限公司 | Quadrivalent influenza virus subunit vaccine safety experiment table for research and development |
| CN116273251A (en) * | 2023-02-14 | 2023-06-23 | 北京赛赋医药研究院有限公司 | Sterile operation device for stem cell preparation and application method thereof |
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| CN203440380U (en) * | 2013-09-05 | 2014-02-19 | 天津开发区合普工贸有限公司 | Integrated zero-gas-leakage microorganism isolation operation cabinet with positive and negative pressure |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107412818A (en) * | 2015-09-11 | 2017-12-01 | 洛阳佳嘉乐农产品开发股份有限公司 | A kind of efficient, the intensive ozone workplace of planting edible mushroom sterilizing |
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| CN105858578A (en) * | 2016-05-26 | 2016-08-17 | 深圳市廖氏原生物医学科技有限公司 | Specimen stationary liquid taking instrument |
| CN105858578B (en) * | 2016-05-26 | 2018-08-28 | 深圳市廖氏原生物医学科技有限公司 | A kind of sample fixer takes liquid instrument |
| CN107523496A (en) * | 2017-10-18 | 2017-12-29 | 安徽理工大学 | A kind of installation for fermenting of comprehensive environmental experiment |
| CN107714358A (en) * | 2017-10-30 | 2018-02-23 | 潍坊潍医医院 | A kind of helium oxygen mixing automatic feeding system |
| CN114768914A (en) * | 2022-05-12 | 2022-07-22 | 江苏中慧元通生物科技股份有限公司 | Quadrivalent influenza virus subunit vaccine safety experiment table for research and development |
| CN114768914B (en) * | 2022-05-12 | 2022-10-21 | 江苏中慧元通生物科技股份有限公司 | Quadrivalence influenza virus subunit vaccine safety experiment table for research and development |
| CN116273251A (en) * | 2023-02-14 | 2023-06-23 | 北京赛赋医药研究院有限公司 | Sterile operation device for stem cell preparation and application method thereof |
| CN116273251B (en) * | 2023-02-14 | 2023-08-25 | 北京赛赋医药研究院有限公司 | Sterile operation device for stem cell preparation and application method thereof |
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| CN103436443B (en) | 2015-11-04 |
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