CN103424559A - Detection cell system used for full-automatic free radical detector - Google Patents
Detection cell system used for full-automatic free radical detector Download PDFInfo
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- CN103424559A CN103424559A CN2012101608509A CN201210160850A CN103424559A CN 103424559 A CN103424559 A CN 103424559A CN 2012101608509 A CN2012101608509 A CN 2012101608509A CN 201210160850 A CN201210160850 A CN 201210160850A CN 103424559 A CN103424559 A CN 103424559A
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Abstract
The invention relates to a detection cell system used for a full-automatic free radical detector. The detection cell system comprises a control device, a sample loading device, a heating device, a detection device, a cleaning device and a reaction container. The control device is in circuit connection with the sample loading device, the heating device, the detection device and the cleaning device respectively. The sample loading device is in pipeline connection with the reaction container. The cleaning device and the heating device are both arranged in the wall of the reaction container. The detection device is arranged and inserted in the reaction container. A drain hole is arranged at the bottom of the reaction container. The detection cell system is designed skillfully, can realize full automation of sample loading, incubation, detection and cleaning. The detection cell system solves problems of complex and tedious sample preparation and being unable to realize full automation and the like for free radical detection before by employing a photochemical luminescence method, and is suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to free radical detector technical field, particularly full-automatic free radical detector technical field, specifically refer to a kind of for the detection cell system on full-automatic free radical detector.
Background technology
Free radical is the atom that contains unpaired electron, molecule or atomic group, it is that mitochondria produces in the process of electronics transmission, under normal circumstances, the radicals scavenging system of body can make the generation of free radical and elimination remain on extremely low mobile equilibrium level, to body, be favourable, but when the body accumulation excessive free radicals, it can damage cell, cause the oxidation of animal tissue or cell, lipid on the infringement cell membrane and protein etc., thereby the cell membrane viscosity is increased, mobility descends, the antioxidase loss of activity, cause the inside and outside ion-exchange disorder of film etc., cause the intracellular calcium overload, xanthine oxidase activity raises, further accelerate again the generation of free radical.For improving the diagnosis efficiency of radical pair animal body damage, the free radical detection method develops rapidly in pathology, biology in recent years, and is widely applied.Detection method commonly used has following several:
1, the mensuration of free radical relevant enzyme: the mensuration of the mensuration of (1) superoxide dismutase, (2) nitrite forming method, (3) polarographic oxygen electrode method, (4) catalatic mensuration, (5) glutathione peroxidase;
2, chemical luminescent detecting;
3, the intermediate product of fluorescence spectrophotometry free radical;
4, fluorescence method;
5, vapor-phase chromatography.
The most free radical detectors of existing market are used following method of operating: manual operation adds reaction utensil by blood sample, reagent, mix and be incubated to by hand the temperature of reaction regulation, by photomultiplier, detect, after detection, discarded one-time reaction ware or use cleaning fluid cleaning reaction ware, prepare test next time.
Therefore, the cleaning in the free radical detector, detect at present, hatch entirely and complete by manual work, complicated tediously long sample preparation, whether reaction vessel cleans up, reagent filling precision, consuming time wait links due to handwork the accuracy on assay bring impact.
Therefore, need to provide a kind of for the detection cell system on full-automatic free radical detector, thereby can realize application of sample, hatch, detect, clean full-automation, and solve the complicated tediously long sample preparation of free radical detector needs in the past, can't realize the limitation such as full-automatic.
Summary of the invention
In order to solve the problem of above-mentioned existence, the purpose of this invention is to provide a kind of for the detection cell system on full-automatic free radical detector, should be ingenious for the detection cell system on full-automatic free radical detector, can realize application of sample, hatch, detect, clean full-automation, and employing photochemiluminescence method has solved free radical in the past and has detected the sample preparation that needs complexity tediously long, can't realize the limitation such as full-automatic, be suitable for large-scale promotion application.
To achieve these goals, of the present invention for the detection cell system on full-automatic free radical detector, be characterized in, comprise control device, sample adding device, heating arrangement, pick-up unit, cleaning device and reaction vessel, described control device circuit respectively connects described sample adding device, described heating arrangement, described pick-up unit and described cleaning device, described sample adding device pipeline connects described reaction vessel, described cleaning device and described heating arrangement all are arranged in the wall of described reaction vessel, described pick-up unit is plugged in described reaction vessel, described reaction container bottom is provided with osculum.
Preferably, described sample adding device comprises sampling probe, sample-adding pump, the application of sample delivery pipe, the first reagent stopple coupon, the first reagent transfer pump, the first reagent delivery pipe, the second reagent stopple coupon, the second reagent transfer pump and the second reagent delivery pipe, sampling probe is connected described reaction vessel by described sample-adding pump with described application of sample delivery pipe pipeline successively, described the first reagent stopple coupon is connected described reaction vessel by described the first reagent transfer pump with described the first reagent delivery pipe pipeline successively, described the second reagent stopple coupon is connected described reaction vessel by described the second reagent transfer pump with described the second reagent delivery pipe pipeline successively, described sampling probe is connected with described control device, described control device circuit respectively connects described sample-adding pump, described the first reagent transfer pump and described the second reagent transfer pump.
More preferably, described sampling probe, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe all adopt titanium alloy material to make, described titanium alloy material be take Titanium as main, and in described titanium alloy material, the weight percentage scope of Titanium is 50%～99.9%.
More preferably, the internal diameter of described sampling probe, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe is 0.8mm – 1.1mm, and external diameter is 1.5mm – 1.8mm.
More preferably, describedly for the detection cell system on full-automatic free radical detector, also comprise reagent trough, described reagent trough respectively with described the first reagent stopple coupon and described the second reagent stopple coupon fluid connection.
More preferably, described cleaning device comprises ultrasonic cleaning agency, rinse bath and cleaning water source, described ultrasonic cleaning agency is arranged in the wall of described reaction vessel, described rinse bath is provided with into water slotted eye and water outlet slotted eye, described cleaning water supply line connects described water inlet slotted eye, described control device circuit connects described ultrasonic cleaning agency, and described sampling probe and described water outlet slotted eye are equipped with for be plugged in described water outlet slotted eye or described sampling probe and described water inlet slotted eye when cleaning and are equipped with for be plugged in described water inlet slotted eye when cleaning.
Further, described ultrasonic cleaning agency comprises ultrasonic transducer and ultrasonic probe, and described ultrasonic probe is arranged on described ultrasonic transducer bottom and wears in described reaction vessel, and described control device circuit connects described ultrasonic transducer.
Further, the top of described water inlet slotted eye or described water outlet slotted eye is the heparin tube placement section.
Further, described ultrasonic cleaning agency becomes 30 degree-90 degree to arrange with described reaction vessel.
Preferably, described heating arrangement is heating element.
Preferably, described pick-up unit comprises silica fibre and photomultiplier, and described silica fibre is plugged in described reaction vessel and by described photomultiplier circuit and connects described control device.
Preferably, describedly for the detection cell system on full-automatic free radical detector, also comprise vibroseis, described vibroseis is arranged on the wall of described reaction vessel and circuit connects described control device.
More preferably, described vibroseis is cam motor.
The present invention is applicable to full-automatic free radical detector, and due to the employing of present technique, the present invention has following beneficial effect:
(1) adopted the photochemiluminescence method, repeatability is better, and Measuring Time is short, with spectroscopic methodology, chromatography commonly used, good comparability is arranged.
(2) do not need complicated tediously long sample preparation, cleaning, detection, incubation are realized robotization fully.
The accompanying drawing explanation
Fig. 1 is the sectional perspective schematic diagram of the specific embodiment for the detection cell system on full-automatic free radical detector of the present invention.
Fig. 2 is that the part master of the specific embodiment shown in Fig. 1 looks schematic diagram.
Fig. 3 is the cross-sectional schematic of the A-A position of structure shown in Fig. 2.
Embodiment
In order more clearly to understand technology contents of the present invention, the spy enumerates following specific embodiment and describes in detail.Yet specific embodiment is only used for illustrating, rather than limitation of the present invention.
Refer to shown in Fig. 1-Fig. 3, of the present inventionly for the detection cell system on full-automatic free radical detector, comprise the control device (not shown), sample adding device 1, heating arrangement 2, pick-up unit 3, cleaning device 4 and reaction vessel 5, described control device circuit respectively connects described sample adding device 1, described heating arrangement 2, described pick-up unit 3 and described cleaning device 4, described sample adding device 1 pipeline connects described reaction vessel 5, described cleaning device 4 and described heating arrangement 2 all are arranged in the wall of described reaction vessel 5, described pick-up unit 3 is plugged in described reaction vessel 5, described reaction vessel 5 bottoms are provided with osculum 51.
Described sample adding device 1 can be any suitable structure, specific design according to specific needs, shown in Figure 1, in specific embodiments of the invention, described sample adding device 1 comprises sampling probe 11, sample-adding pump 12, application of sample delivery pipe (not shown), the first reagent stopple coupon (not shown), the first reagent transfer pump 13, the first reagent delivery pipe (not shown), the second reagent stopple coupon (not shown), the second reagent transfer pump 14 and the second reagent delivery pipe (not shown), sampling probe 11 is connected described reaction vessel 5 by described sample-adding pump 12 with described application of sample delivery pipe pipeline successively, described the first reagent stopple coupon is connected described reaction vessel 5 by described the first reagent transfer pump 13 with described the first reagent delivery pipe pipeline successively, described the second reagent stopple coupon is connected described reaction vessel 5 by described the second reagent transfer pump 14 with described the second reagent delivery pipe pipeline successively, described sampling probe 11 is connected with described control device, described control device circuit respectively connects described sample-adding pump 12, described the first reagent transfer pump 13 and described the second reagent transfer pump 14.
In order to make application of sample more accurate, in specific embodiments of the invention, described sampling probe 11, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe all adopt titanium alloy material to make, described titanium alloy material be take Titanium as main, and in described titanium alloy material, the weight percentage scope of Titanium is 50%～99.9%.Described titanium alloy material is 1/10000000th through cleaning residual rate, and the delivery pipe that traditional material is made is titanium alloy delivery pipe more than thousand times of residual rate after cleaning cleaning residual rate later.
The internal diameter of described sampling probe 11, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe and external diameter are determined as required, in specific embodiments of the invention, the internal diameter of described sampling probe 11, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe is 0.8mm – 1.1mm, and external diameter is 1.5mm – 1.8mm.
In order to improve automaticity, in specific embodiments of the invention, describedly for the detection cell system on full-automatic free radical detector, also comprise the reagent trough (not shown), described reagent trough respectively with described the first reagent stopple coupon and described the second reagent stopple coupon fluid connection.
Described cleaning device 4 can adopt any suitable structure, refer to shown in Fig. 1-Fig. 3, in specific embodiments of the invention, described cleaning device 4 comprises ultrasonic cleaning agency 41, rinse bath 42 and cleaning water source (not shown), described ultrasonic cleaning agency 41 is arranged in the wall of described reaction vessel 5, described rinse bath 42 is provided with into water slotted eye (not shown) and water outlet slotted eye (not shown), described cleaning water supply line connects described water inlet slotted eye, described control device circuit connects described ultrasonic cleaning agency 41, described sampling probe 11 is equipped with for be plugged in described water outlet slotted eye or described sampling probe 11 and described water inlet slotted eye when cleaning and is equipped with for be plugged in described water inlet slotted eye when cleaning with described water outlet slotted eye.
Described ultrasonic cleaning agency 41 can adopt any suitable structure, shown in refer to-Fig. 3, in specific embodiments of the invention, described ultrasonic cleaning agency 41 comprises ultrasonic transducer 43 and ultrasonic probe 44, described ultrasonic probe 44 is arranged on described ultrasonic transducer 43 bottoms and wears in described reaction vessel 5, and described control device circuit connects described ultrasonic transducer 43.
In order to be convenient to place heparin tube simultaneously, preferably, the top of described water inlet slotted eye or described water outlet slotted eye is the heparin tube placement section.In specific embodiments of the invention, the top of described water outlet slotted eye is the heparin tube placement section.Certainly, also the heparin tube placement section can be set in addition.
Described ultrasonic cleaning agency 41 can become any suitable angle setting with described reaction vessel 5, and preferably, 5 one-tenth 30 degree of described ultrasonic cleaning agency 41 and described reaction vessel-90 degree arrange.Refer to shown in Fig. 1 and Fig. 3, in specific embodiments of the invention, 5 one-tenth 90 degree of described ultrasonic cleaning agency 41 and described reaction vessel arrange.
Described heating arrangement 2 can adopt any suitable structure, refers to shown in Fig. 1-Fig. 3, and in specific embodiments of the invention, described heating arrangement 2 is heating elements.
Described pick-up unit 3 can adopt any suitable structure, shown in refer to-Fig. 3, in specific embodiments of the invention, described pick-up unit 3 comprises silica fibre 31 and photomultiplier (not shown), and described silica fibre 31 is plugged in described reaction vessel 5 and by described photomultiplier circuit and connects described control device.
For the reactant in abundant mixed reaction vessel 5, refer to shown in Fig. 1-Fig. 3, in specific embodiments of the invention, describedly for the detection cell system on full-automatic free radical detector, also comprise vibroseis 6, described vibroseis 6 is arranged on the wall of described reaction vessel 5 and circuit connects described control device.
Described vibroseis 6 can be any suitable parts, refers to shown in Fig. 1-Fig. 3, and in specific embodiments of the invention, described vibroseis 6 is cam motors.
In specific embodiments of the invention, reaction vessel 5 inside can be reaction utensil, and top and silica fibre 31 1 ends are fixed, and form the darkroom structure.Silica fibre 31 other ends connect photomultiplier fixing.
Use procedure of the present invention is roughly as follows: the top that heparin tube 7 is placed on to the water outlet slotted eye, sample-adding pump 12 work, the control device for example Single-chip Controlling sampling probe on the level detection plate 11 inserts heparin tubes 7 and is sampled, serum is transferred in reaction vessel 5, application of sample finishes, remove heparin tube 7, control device for example Single-chip Controlling sampling probe 11 automatically explores downward in the water outlet slotted eye and is cleaned.The first reagent transfer pump 13 and the second reagent transfer pump 14 are taked respectively antioxidant and luminescent solution from reagent trough, transfer in reaction vessel 5.Incubation is carried out in heating element work in the wall of reaction vessel 5; Cam motor on the wall of reaction vessel 5 produces vibrations by the interior liquid blending of reaction vessel 5.Be fixed on silica fibre 31 photon transmission in reaction vessel 5 to photomultiplier, then convert light signal to electric signal and reach control device, then be delivered in computing machine and detect analysis, draw data, detect free-radical contents in human body.The liquid that reaction vessel 5 will detect is discharged from the osculum 51 of bottom.Sampling probe 11 explores downward in the water outlet slotted eye automatically, and sample-adding pump 12 work are drawn pure water, by pipeline, pure water injected to reaction vessel 5 automatically, and sampling probe 11 inside and outside walls are rinsed totally simultaneously.Ultrasonic cleaning agency 41 work, ultrasonic probe 44 enters the pure water that is arranged in reaction vessel 5 and carries out ultrasonic cleaning.The detection cell system is carried out next cycle detection.
To sum up, of the present invention ingenious for the detection cell system on full-automatic free radical detector, can realize application of sample, hatch, detect, clean full-automation, and employing photochemiluminescence method has solved free radical in the past and has detected the sample preparation that needs complexity tediously long, can't realize the limitation such as full-automatic, be suitable for large-scale promotion application.
In this instructions, the present invention is described with reference to its specific embodiment.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, instructions and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (13)
1. one kind for the detection cell system on full-automatic free radical detector, it is characterized in that, comprise control device, sample adding device, heating arrangement, pick-up unit, cleaning device and reaction vessel, described control device circuit respectively connects described sample adding device, described heating arrangement, described pick-up unit and described cleaning device, described sample adding device pipeline connects described reaction vessel, described cleaning device and described heating arrangement all are arranged in the wall of described reaction vessel, described pick-up unit is plugged in described reaction vessel, described reaction container bottom is provided with osculum.
2. according to claim 1 for the detection cell system on full-automatic free radical detector, it is characterized in that, described sample adding device comprises sampling probe, sample-adding pump, the application of sample delivery pipe, the first reagent stopple coupon, the first reagent transfer pump, the first reagent delivery pipe, the second reagent stopple coupon, the second reagent transfer pump and the second reagent delivery pipe, sampling probe is connected described reaction vessel by described sample-adding pump with described application of sample delivery pipe pipeline successively, described the first reagent stopple coupon is connected described reaction vessel by described the first reagent transfer pump with described the first reagent delivery pipe pipeline successively, described the second reagent stopple coupon is connected described reaction vessel by described the second reagent transfer pump with described the second reagent delivery pipe pipeline successively, described sampling probe is connected with described control device, described control device circuit respectively connects described sample-adding pump, described the first reagent transfer pump and described the second reagent transfer pump.
3. according to claim 2 for the detection cell system on full-automatic free radical detector, it is characterized in that, described sampling probe, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe all adopt titanium alloy material to make, described titanium alloy material be take Titanium as main, and in described titanium alloy material, the weight percentage scope of Titanium is 50%～99.9%.
4. according to claim 2 for the detection cell system on full-automatic free radical detector, it is characterized in that, the internal diameter of described sampling probe, described application of sample delivery pipe, described the first reagent stopple coupon, described the first reagent delivery pipe, described the second reagent stopple coupon and described the second reagent delivery pipe is 0.8mm – 1.1mm, and external diameter is 1.5mm – 1.8mm.
5. according to claim 2 for the detection cell system on full-automatic free radical detector, it is characterized in that, describedly for the detection cell system on full-automatic free radical detector, also comprise reagent trough, described reagent trough respectively with described the first reagent stopple coupon and described the second reagent stopple coupon fluid connection.
6. according to claim 2 for the detection cell system on full-automatic free radical detector, it is characterized in that, described cleaning device comprises ultrasonic cleaning agency, rinse bath and cleaning water source, described ultrasonic cleaning agency is arranged in the wall of described reaction vessel, described rinse bath is provided with into water slotted eye and water outlet slotted eye, described cleaning water supply line connects described water inlet slotted eye, described control device circuit connects described ultrasonic cleaning agency, described sampling probe and described water outlet slotted eye are equipped with for be plugged in described water outlet slotted eye or described sampling probe and described water inlet slotted eye when cleaning and are equipped with for be plugged in described water inlet slotted eye when cleaning.
7. according to claim 6 for the detection cell system on full-automatic free radical detector, it is characterized in that, described ultrasonic cleaning agency comprises ultrasonic transducer and ultrasonic probe, described ultrasonic probe is arranged on described ultrasonic transducer bottom and wears in described reaction vessel, and described control device circuit connects described ultrasonic transducer.
8. according to claim 6ly for the detection cell system on full-automatic free radical detector, it is characterized in that, the top of described water inlet slotted eye or described water outlet slotted eye is the heparin tube placement section.
9. according to claim 6ly for the detection cell system on full-automatic free radical detector, it is characterized in that, described ultrasonic cleaning agency becomes the setting of 30 degree-90 degree with described reaction vessel.
10. according to claim 1ly for the detection cell system on full-automatic free radical detector, it is characterized in that, described heating arrangement is heating element.
11. according to claim 1 for the detection cell system on full-automatic free radical detector, it is characterized in that, described pick-up unit comprises silica fibre and photomultiplier, and described silica fibre is plugged in described reaction vessel and by described photomultiplier circuit and connects described control device.
12. according to claim 1 for the detection cell system on full-automatic free radical detector, it is characterized in that, describedly for the detection cell system on full-automatic free radical detector, also comprise vibroseis, described vibroseis is arranged on the wall of described reaction vessel and circuit connects described control device.
13. according to claim 12, for the detection cell system on full-automatic free radical detector, it is characterized in that, described vibroseis is cam motor.
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| CN2012101608509A CN103424559A (en) | 2012-05-17 | 2012-05-17 | Detection cell system used for full-automatic free radical detector |
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| CN2012101608509A CN103424559A (en) | 2012-05-17 | 2012-05-17 | Detection cell system used for full-automatic free radical detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113227348A (en) * | 2018-12-20 | 2021-08-06 | 欧姆尼欧美公司 | Temperature control for analysis of nucleic acids and other analytes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113227348A (en) * | 2018-12-20 | 2021-08-06 | 欧姆尼欧美公司 | Temperature control for analysis of nucleic acids and other analytes |
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Application publication date: 20131204 |