CN103421835A - Application of transcription factor gene RrMYB11 in regulating and controlling rose types - Google Patents
Application of transcription factor gene RrMYB11 in regulating and controlling rose types Download PDFInfo
- Publication number
- CN103421835A CN103421835A CN2012105403495A CN201210540349A CN103421835A CN 103421835 A CN103421835 A CN 103421835A CN 2012105403495 A CN2012105403495 A CN 2012105403495A CN 201210540349 A CN201210540349 A CN 201210540349A CN 103421835 A CN103421835 A CN 103421835A
- Authority
- CN
- China
- Prior art keywords
- gene
- rrmyb11
- rose
- plant
- transcription factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of plant genetic engineering, and particularly relates to separation, clone, functional identification and application of a rose DNA fragment. The rose DNA fragment comprises a rose transcription factor gene RrMYB11, wherein the nucleotide sequence of the rose transcription factor gene RrMYB11is shown in a sequence list SEQ ID NO: 1, and the corresponding protein sequence is shown in SEQ ID NO: 2. The gene fragment can change the flower type of a plant, and can be transferred into the plant directly to change the flower type of the transgenic plant.
Description
Technical field
The present invention relates to the plant gene engineering technology field.Be specifically related to a kind of separating clone, functional verification and application of rose gene fragment.Described gene is grown relevant with plant petals.The rear general plant materials that directly proceeds to is combined in the complete translation district of this gene with cauliflower mosaic virus promoter, the petal of transfer-gen plant is grown and the flower type changes.
Background technology
Transcription factor also claims trans-acting factor, refer to can with the interactional DBP of cis-acting elements generation specificity in gene promoter region, by between them and and other associated protein between interaction activate or suppress (the Zhang Chunyu that transcribes of some gene, dragon is gorgeous, Feng Ji, regulation and control and the biological significance thereof of Meng Jinling plant gene on transcriptional level. hereditary .2007,29 (7) 793-799).
Myb gene family is one of maximum plant transcription factor gene family, and they all have the DNA of high conservative in conjunction with territory-MYB structural domain.Each MYB structural domain is by approximately 52 amino-acid residues and intervening sequence form, every one, 18-19 amino acid interval tryptophan residue, these spatially regularly arranged amino-acid residue make the MYB structural domain be folded into spiral-corner one spiral (Helix-Turn-Helix, HTH) structure, MYB albumen inserts target DNA molecule major groove by this structure is combined (Frampton J with target DNA, eds.Myb transcription factors:their role in growth, differentiation and disease, Kluwer academic publishers, Springer, Netherlands, pp.6-8, 2004).Number according to contained MYB structural domain, myb transcription factor in plant simply can be divided into to single MYB domain protein (R1/2, R3), 2RMYB albumen (R2R3), 3RMYB albumen (R1R2R3) and 4RMYB (R1R2R3R4) (Stracke R, Werber M, and Weisshaar B.The R2R3-MYB gene family in Arabidopsis thaliana.Curr.Opin.Plant Biol, 2001,4:447-456).In plant materials, most of MYB albumen has the MYB structural domain (R2R3) of 2 repetitions.
In plant, as far back as 1987, Paz-Ares just successfully found the MYBC1 gene (Paz-Ares et al., 1987) relevant to pigment synthesis from corn.Subsequently, in various plants as Common Snapdragon (Waites et al., 1998), petunia (Walker et al1999), soybean (Miyake et al., 2003), Arabidopis thaliana (Chen et al., 2006), grape (Deluc et al., 2006) and in apple (Takos et al., 2006) etc. also isolation identification go out the MYB albumen of Various Functions.
MYB class transcription factor has almost participated in all respects of development of plants and metabolism.They can with other transcription factor family member transcription factor interaction, the formation of the mode regulating plant cell by combinatorial regulation and pattern formation, the particularly phenylalanine metabolism of regulating plant secondary metabolism, and environment, hormone and disease and pest etc. are made and should be beaten with a stick to external world.
Rose is the important economic plants of China, is also important viewing and admiring and one of production amphitypy flowers in the world.Up to the present, also there is no the report about myb transcription factor in rose.
Summary of the invention
Purpose of the present invention provides a kind of application of transcription factor gene RrMYB11 in regulation and control rose of separation, and this gene is relevant to the formation of the shape of petal.
The invention provides gene coded sequence and the function thereof of the transcription factor RrMYB11 of MYB family expressed in rose, specifically comprise: the clone of the nucleotide coding sequence of RrMYB11 gene, expression vector establishment, and transform in this gene and tobacco, carry out Molecular Identification and Phenotypic Observation.
At first the present invention clones a kind of new MYB family transcription factor gene from rose, by its called after RrMYB11.This gene fragment is the DNA molecular with particular sequence, and its open reading frame is 669bp, and its nucleotide sequence is as shown in sequence table SEQ ID NO:1.
The present invention also provides this rose RrMYB11 albumen coded sequence, and it has 222 amino-acid residues, and its corresponding aminoacid sequence is shown in SEQ ID NO.1, and the aminoacid sequence of the protein that it is corresponding is as shown in SEQ ID NO:2.
The present invention also provides a pair of nucleotide primer that obtains rose sample gene RrMYB11 for transferring.This primer, according to gene RrMYB11 design, is used this to primer pair roseleaf sample cDNA, to carry out the gene fragment that pcr amplification can obtain 727bp.The DNA sequence dna of this primer pair is as follows:
P1 forward primer: 5 ' GCAAGAAATCACTGAGGAATAGGG3 ' (seeing sequence table SEQ ID NO:3)
P2 reverse primer: 5 ' GCTTTAACTACAAACTGAAACGAGGC3 ' (seeing sequence table SEQ ID NO:4)
The nucleotide primer that the present invention also provides a pair of detection rose RrMYB11 gene to express in transgene tobacco.Whether this primer, according to gene RrMYB11 design, is used this tobacco sample cDNA that primer pair is transformed to this gene to carry out the RT-PCR amplification, detect this gene and express in transgene tobacco, can obtain the gene fragment of 564bp.The DNA sequence dna of this primer pair is:
P3 forward primer: 5 ' CAAAGCAGGTCTAAACCGATGTGG3 ' (seeing sequence table SEQ ID NO:5)
P4 reverse primer: 5 ' GCTTTAACTACAAACTGAAACGAGGC3 ' (seeing sequence table SEQ ID NO:6)
Can utilize any carrier that can guide foreign gene to express in plant, by directly delivered DNA, electricity lead, the conventional biotechnological means such as agriculture bacillus mediated imports vegetable cell or tissue by the encoding gene of RrMYB11 provided by the invention, and the plant tissue of conversion cultivated into to plant.While using gene fragment of the present invention to be building up in plant expression vector, can add that in its transcription initiation Nucleotide front any one strengthens promotor or inducible promoter.For the ease of transgenic plant cells or plant are identified and are screened, can be processed used carrier, there is the antibiotic marker thing (such as kantlex or Totomycin etc.) of resistance as added.The host who is converted is the various plants that comprises tobacco, cultivates the floristics of the type that do not suit.
The accompanying drawing explanation
Sequence table SEQ ID NO:1 is the DNA fragmentation that includes the RrMYB11 gene coding region (wherein 63-731bp is CDS) of separating clone of the present invention.The sequence total length is 721bp, 222 amino acid of encoding.
Sequence table SEQ ID NO:2 is the aminoacid sequence of protein corresponding to above-mentioned RrMYB11 transcription factor gene fragment.
Sequence table SEQ ID NO:3, SEQ ID NO:4 is the DNA sequence dna of the primer pair of the above-mentioned roseleaf sample cDNA of amplification.
Sequence table SEQ ID NO:5, SEQ ID NO:6 is the DNA sequence dna that detects expressing gene fragment (564b) primer pair in transgene tobacco.
Fig. 1: be existing plant expression vector pCAMBIA2300s structural representation.
Fig. 2: be the recombinant plasmid pCAMBIA2300s-RrMYB11 structural representation that the present invention builds.
Fig. 3: the colored type comparison diagram of the tobacco (transgenosis) of contrast wild-type tobacco (non-transgenic) and conversion RrMYB11 gene.In figure: Fig. 3 A is the colored type that contrasts not genetically modified plant (tobacco); Fig. 3 B is the colored type of transgenic plant of the present invention (tobacco).
Fig. 4: the expression amount situation map of RrMYB11 gene in transfer-gen plant.In figure: the positive contrast of P, Water is the distilled water contrast, CK is that (non-transgenic, as the contrast of transgene tobacco, all the other are transgenosis independence transfer-gen plant to Nicotiana gossei.
Embodiment
Embodiment 1: separating clone RrMYB11 gene
(again claim " No. one, Pingyin " to rich colored rose early stage of the present invention, http://tc.cctv.com/20100412/103621.shtml, the seed selection document of rich colored rose is published, see: Lv Chuanrun (Pingyin rose institute), New Variety of Rugosa-Rose-Feng flower rose and cultivation technique, the Shandong forestry science and technology, 2007,5:77; Gardening forestry institute of Hua Zhong Agriculture University gardening plant biology flowers Practical Teaching Base is introduced a fine variety from Pingying County Pingyin, Shandong Province rose institute) flower of different times carried out transcribing group order-checking (transcribing the group order-checking is completed by Shenzhen Huada Genetic Technology Co., Ltd), obtained the full length sequence (seeing GenBank accession number: FR828544.1, http://www.ncbi.nlm.nih.gov/nuccore/FR828544.1) of RrMYB11 gene in transcribing the group sequencing result.According to the known sequence of this gene transcript sequencing RrMYB11 designed specific primers P1 (see sequence listing SEQ, ID, NO: 3), forward primer 5'GCAAGAAATCACTGAGGAATAGGG3 'and P2 (see sequence listing SEQ, ID, NO: 4) reverse primer 5'GCTTTAACTACAAACTGAAACGAGGC3 ', will sequencing of 31-757bp from the rose varieties' amplified Floribunda' (ie Floribunda Rose) petals RNA was reverse transcribed in cDNA fragment was amplified as follows:GCAAGAAATCACTGAGGAATAGGGAAGTGGCAATGGTGCCATTAAGCACTAGAAGTTACAAGAACAAGGAAGTAAACAGAGGGTCTTGGACAGCAGAAGAAGATCAGAGACTGGCTCAAGTTATTGAAGTCCATGGCCCAAGAAAGTGGAAGTCTGTTGCAACCAAAGCAGGTCTAAACCGATGTGGGAAGAGTTGCAGACTGAGATGGATGAACTATCTTAGACCAAATATTAAGAGAGGCAATATATCAGACCAAGAAGAGGACTTGATACTCAGGCTTCATAAACTCCTTGGAAACAGGTGGTCATTGATTGCCGGAAGACTGCCTGGTCGAACAGACAACGAGATTAAGAATTACTGGAACTCTCATTTGAGCAAGAAGATCAAGCATAACGAGAGATCACAAAAACAAAACAGACTACTTTCTGCATCATCAAATCCAGCAGCACAAGAGTTGTCCTCCTCGTCCGAGCCTCAGAATGTTGAGTTAATGGAGAACCATGCTGTACCAATTGGAATAGAAGATGACAGCTCGAAACGCGAGGAGAACTACTTCTTCAAGTCCATGAGCTATAATGGATCATCTGGAGATGAGTTCTTGTTTGATGGCTCAACTGCTGACGAGGGGCCTTTGAATTTGGAGTGGATGAATAGATTTCTAGAAATGGATGAGAGTTGGTTCACTCTGCATGACATTTGAGCCTCGTTTCAGTTTGTAGTTAAAGC
32bp-701bp in amplified production is exactly sequence of the present invention.Concrete steps are:
1, adopt CTAB method commonly used (reference: " plant genetic engineering ", Wang Guanlin, Fang Hongjun chief editor) to extract the total RNA of petal from rose variety ' rich flower ', concrete steps are as follows:
1) add CTAB (cetyl trimethylammonium bromide) Extraction buffer (2% (W/V) CTAB in centrifuge tube, NaCl1.4mol/L, EDTA (ethylenediamine tetraacetic acid (EDTA)) 20mmol/L, TrisCl 100mmol/L, 2% (W/V) pvp) and 10% beta-mercaptoethanol, preheating in water-bath.
2) roseleaf is ground by cooled with liquid nitrogen, add in extracting solution, mix, 65 ℃ of water-baths 10 minutes
3) add isopyknic chloroform: primary isoamyl alcohol (volume ratio 24: 1) mixed solution, put upside down and mix, standing 10min, 4 ℃ of centrifugal 10min of lower 12000g
4) get supernatant, repeating step 3)
5) get supernatant, add the LiCl that final concentration is 2mol/L, ice bath 10-12 hour, 12000g, 4 ℃ centrifugal 15 minutes, abandon supernatant, by 75% ethanol washing and precipitating twice, be dissolved in appropriate DEPC (diethylpyrocarbonate) and process in water stand-by.
6) take from rose variety ' rich flower ' (being rich colored rose) that to extract the total RNA of petal be template, utilize ThermoScript II (purchased from precious biotechnology Dalian company limited) by the synthetic cDNA article one chain of its reverse transcription, reaction conditions is: 65 ℃ of 5min, 42 ℃ of 50min, 70 ℃ of 10min.
7), according to special primer P1 (the seeing sequence table SEQ ID NO:3) 5 ' GCAAGAAATCACTGAGGAATAGGG3 ' and P2 (the seeing sequence table SEQ ID NO:4) 5 ' GCTTTAACTACAAACTGAAACGAGGC3 ' that transcribe the sequences Design in order-checking, the cDNA that RrMYB11 is obtained from rose variety ' rich flower ' petal RNA reverse transcription, amplification out.
Reaction conditions: 94 ℃ of denaturation 4min; 94 ℃ of 30sec, 60 ℃ of 30sec, 72 ℃ of 1min, 37 circulations; 72 ℃ are extended 10min.The PCR product that amplification is obtained is connected into
Carrier (purchased from precious biotechnology Dalian company limited), screening positive clone order-checking, obtain required full-length gene.This clone is named as
Plasmid.
The structure of embodiment 2:RrMYB11 gene overexpression carrier, transform
MYB class transcription factor has almost participated in all respects of development of plants and metabolism, in order to illustrate better the function of this gene, applies for its overexpression in tobacco is verified from the phenotype of transfer-gen plant.Concrete steps are: at first by the positive colony obtained in embodiment 1
BamH I and Sal I double digestion for plasmid, reclaim the purpose fragment; Simultaneously, the enzyme that uses the same method is cut the genetic transformation carrier pCAMBIA2300s (this genetic transformation carrier builds and gives from the Wuhan City, Hubei Province State Key Laboratory of Crop Genetic Improvent) that carries two tobacco mosaic disease virus promoter 35S.Enzyme cuts complete, and pCAMBIA2300s (Fig. 1) carrier of cutting with the endonuclease bamhi that comprises the RrMYB11 gene and enzyme is done ligation, transforms bacillus coli DH 5 alpha (coli strain is purchased from precious biotechnology Dalian company limited).Cut screening positive clone by enzyme, obtain recombinant plasmid (or claiming conversion carrier), by its called after pCAMBIA2300s-RrMYB11 (seeing Fig. 2).
By agriculture bacillus mediated tobacco genetic transforming method, it is imported in tobacco, through infecting, cultivate altogether, Transformation of tobacco seedling that screening has kalamycin resistance, then, by taking root, practice the conventional steps such as transplantation of seedlings, obtain transfer-gen plant.
The key step of genetic transformation of the present invention and application reagent are as described below:
(1) reagent and solution abbreviation
In the present invention, the abbreviation of substratum plant hormone used is expressed as follows: 6-BA (6-BenzylaminoPurine, 6-benzyl aminopurine); NAA (Naphthalene acetic acid, naphthylacetic acid); Kan (Kanamycin, kantlex); Cef (Cefotaxime, cephamycin)
(2) for the culture medium prescription of tobacco genetic transformation
Table 1 has been listed composition and the consumption thereof of various substratum of the present invention.
The design of table 1 Transformation of tobacco substratum
Annotate: 1/2MS, the preparation of MS substratum is referring to Murashige T.and F.Skoog.Physiol.Plant, the method for 1962,15:473-497 report.
Kan (Kanamycin in table 1, kantlex), Cef (Cefotaxime, cephamycin), adopt 0.45 μ m membrane filtration method sterilizing, above-mentioned except the substratum Kan and Cef composition after conventional 121 ℃ of high pressure steam sterilization 20min, when substratum is cooled to 50-60 ℃, on Bechtop, add.
(1) agriculture bacillus mediated genetic transformation step
1) cultivation of Agrobacterium
At first, in the upper preculture Agrobacterium EHA10548 hour of the solid LB substratum (10g/L peptone+5g/L yeast extract+10g/L sodium-chlor+Kan100mg/L+ agar 1.5g/L) of selecting with corresponding resistance, 28 ℃ of culture temperature; The single bacterium colony of picking preculture Agrobacterium, be inoculated in the liquid LB substratum (10g/L peptone+5g/L yeast extract+10g/L sodium-chlor+Kan100mg/L) of corresponding resistance selection, in 28 ℃ of 200rpm shaking table overnight incubation, to bacterial concentration OD
600Value is approximately 0.6.
2) leaf disc transformation method
A. the young leaflet tablet that launch fully on clip tobacco aseptic seedling top, be cut into 0.8cm * 0.8cm size fritter by blade, puts into aseptic beaker;
B. pour ready bacterium liquid into beaker, jiggle beaker.Blade soaks 10min in bacterium liquid;
C. the blade in step b is taken out, be transferred on the filter paper of the bacterium of having gone out and blot; Then be placed on dark the cultivation three days on culture medium altogether as above, culture temperature is 28 ℃;
D. after three days, blade is proceeded to as described in Table 1 sprouting and select on substratum, illumination and dark alternately (the intensity of illumination 1000-1500lx that cultivates, light application time: 16h/d, interlunation: 8h/d) the lower cultivation, carry out the screening differentiation of Kam resistant buds, culture temperature is 28 ℃;
E. after resistant buds forms, it is cut, proceed to and select on substratum strong sprout as above, in illumination and dark the cultivation, replace (intensity of illumination 1000-1500lx, light application time: 16h/d, interlunation: 8h/d) the lower cultivation, carry out the screening of Kam resistance seedling, culture temperature is 28 ℃;
F. resistance seedling screening obtained proceeds to as above taking root and selects on substratum, it to be taken root, and in illumination, with dark the cultivation alternately, (interlunation: 8h/d) lower cultivation, culture temperature is 28 ℃ for intensity of illumination 1000-1500lx, light application time: 16h/d.
2) transplant
Wash the residual substratum on the transgenic tobacco plant root off, the seedling that will have good root system proceeds to greenhouse, within an initial week, keeps moisture moistening simultaneously.
Result obtains the positive T that proceeds to recombinant plasmid pCAMBIA2300s-RrMYB11 (referring to Fig. 2) of PCR detected result of 20 strains altogether
0For transgene tobacco.
Embodiment 3:RrMYB11 gene transgenic T
0The phenotype observation of generation in field detects with RT-PCR
After transplanting under transgenic tobacco plant, until flowering period, the colored type of the colored type of transgene tobacco and unconverted tobacco is compared, the colored type that discovery turns the tobacco of RrMYB11 gene changes: there is no the pentagon of tobacco corolla for launching transformed, have no the substantial petal that splits (Fig. 3 A); The tobacco petal that turns the RrMYB11 gene caves in and forms the pentagram type, 5 film clips lobe exterior features of obvious drastic crack occur, and petal tail point is heart type (Fig. 3 B).
Whether relevant with the RrMYB11 gene proceeded to for the change of verifying transgene tobacco flower type, the present invention has adopted RT-PCR method commonly used to carry out detecting (the results are shown in Figure 3) to RrMYB11 genetic expression in the part transgenic tobacco plant.Concrete steps are as follows: adopt TRIZOL reagent (purchased from precious biotechnology Dalian company limited) to extract total RNA (extracting method operates according to above-mentioned TRIZOL reagent specification sheets) of flower from transgene tobacco 1-6 strain, utilize ThermoScript II (purchased from precious biotechnology Dalian company limited) by the synthetic cDNA article one chain of its reverse transcription, reaction conditions is 65 ℃ of 5min, 42 ℃ of 50min, 70 ℃ of 10min.First with the house-keeping gene eIF reported, (accession number: cDNA X79009) reverse transcription obtained detects and the concentration adjustment, sequences Design pair of primers P5 forward primer (5-GCAGCCGTGATCACACAGTATCT) and P6 reverse primer (5-ACACCCTTCCTTCCAAAACGT) according to house-keeping gene EIF, carry out the PCR detection, reaction conditions is: 94 ℃ of denaturation 4min; 94 ℃ of 30sec, 60 ℃ of 30sec, 72 ℃ of 30sec, 28 circulations; 72 ℃ are extended 10min.As shown in Figure 3, house-keeping gene EIF all can expand test-results in wild-type tobacco and transformation of tobacco, and brightness is consistent.Then, sequence according to the RrMYB11 gene, designing pair of primers P3 (seeing sequence table SEQ ID NO:3) forward primer (5-CAAAGCAGGTCTAAACCGATGTGG) and P4 (seeing sequence table SEQ ID NO:4) reverse primer (5-GCTTTAACTACAAACTGAAACGAGGC) near 3 ' end, carry out the RT-PCR detection, reaction conditions is: 94 ℃ of denaturation 4min; 94 ℃ of 30sec, 60 ℃ of 30sec, 72 ℃ of 1min, 35 circulations; 72 ℃ are extended 10min.Test-results shows, the expression of RrMYB11 gene all detected in 6 strain transgene tobaccos, and result as shown in Figure 3.In Fig. 3, show: 1: be the pcr amplification result of plasmid pCAMBIA2300s-RrMYB11,2: be the pcr amplification result of the wild-type tobacco that do not have to transform, 3: be the distilled water contrast, 4-9: be the pcr amplification result of the transgene tobacco that proceeds to recombinant plasmid pCAMBIA2300s-RrMYB11.
Claims (1)
1. the application of the transcription factor gene RrMYB11 of a separation in regulation and control rose, is characterized in that the nucleotide sequence of this gene is as shown in sequence table SEQ ID NO:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210540349.5A CN103421835B (en) | 2012-12-08 | 2012-12-08 | Application of transcription factor gene RrMYB11 in regulating and controlling rose types |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210540349.5A CN103421835B (en) | 2012-12-08 | 2012-12-08 | Application of transcription factor gene RrMYB11 in regulating and controlling rose types |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103421835A true CN103421835A (en) | 2013-12-04 |
| CN103421835B CN103421835B (en) | 2015-03-04 |
Family
ID=49647227
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210540349.5A Expired - Fee Related CN103421835B (en) | 2012-12-08 | 2012-12-08 | Application of transcription factor gene RrMYB11 in regulating and controlling rose types |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103421835B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103993019A (en) * | 2014-04-30 | 2014-08-20 | 华中农业大学 | Application of rosa rugosa RrDFR1 gene to regulate and control synthesis of plant anthocyanidin |
| CN107365778A (en) * | 2017-09-18 | 2017-11-21 | 合肥工业大学 | Regulate and control transcription factor gene and its application of lutein synthesis |
| CN109576282A (en) * | 2018-12-18 | 2019-04-05 | 中国农业大学 | Chinese rose transcription factor RhMYB4 and its development of floral organs regulation in application |
| CN111548400A (en) * | 2020-05-18 | 2020-08-18 | 扬州大学 | Rose fragrance regulatory gene RrMYB114 and application thereof |
| CN111961123A (en) * | 2020-07-09 | 2020-11-20 | 华中农业大学 | Rose RrMYB18 transcription factor and application thereof in promoting plant secondary wall biosynthesis and plant dwarfing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491962A (en) * | 2002-10-25 | 2004-04-28 | 中国科学院遗传与发育生物学研究所 | Myb transcription factor of cotton and its encoding gene and use |
| CN101928337A (en) * | 2010-08-18 | 2010-12-29 | 北京林业大学 | Chrysanthemum drought-tolerant gene and application thereof |
| CN101935663A (en) * | 2010-04-28 | 2011-01-05 | 中国科学院遗传与发育生物学研究所 | New wheat gene TaMYB3 for regulating synthetization and metabolization of anthocyanin |
-
2012
- 2012-12-08 CN CN201210540349.5A patent/CN103421835B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491962A (en) * | 2002-10-25 | 2004-04-28 | 中国科学院遗传与发育生物学研究所 | Myb transcription factor of cotton and its encoding gene and use |
| CN101935663A (en) * | 2010-04-28 | 2011-01-05 | 中国科学院遗传与发育生物学研究所 | New wheat gene TaMYB3 for regulating synthetization and metabolization of anthocyanin |
| CN101928337A (en) * | 2010-08-18 | 2010-12-29 | 北京林业大学 | Chrysanthemum drought-tolerant gene and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| NING.G.G.: "CCA29100", 《GENBANK》, 3 April 2011 (2011-04-03) * |
| 秦宏道: "玫瑰MYB基因的克隆及超量表达载体的构建", 《中国优秀硕士学位论文全文数据库》, 31 May 2012 (2012-05-31), pages 148 - 198 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103993019A (en) * | 2014-04-30 | 2014-08-20 | 华中农业大学 | Application of rosa rugosa RrDFR1 gene to regulate and control synthesis of plant anthocyanidin |
| CN103993019B (en) * | 2014-04-30 | 2016-05-04 | 华中农业大学 | The application of rose RrDFR1 gene in regulating plant anthocyanidin is synthetic |
| CN107365778A (en) * | 2017-09-18 | 2017-11-21 | 合肥工业大学 | Regulate and control transcription factor gene and its application of lutein synthesis |
| CN107365778B (en) * | 2017-09-18 | 2020-05-15 | 合肥工业大学 | Transcription factor gene for regulating and controlling lutein synthesis and application thereof |
| CN109576282A (en) * | 2018-12-18 | 2019-04-05 | 中国农业大学 | Chinese rose transcription factor RhMYB4 and its development of floral organs regulation in application |
| CN111548400A (en) * | 2020-05-18 | 2020-08-18 | 扬州大学 | Rose fragrance regulatory gene RrMYB114 and application thereof |
| CN111961123A (en) * | 2020-07-09 | 2020-11-20 | 华中农业大学 | Rose RrMYB18 transcription factor and application thereof in promoting plant secondary wall biosynthesis and plant dwarfing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103421835B (en) | 2015-03-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105441460B (en) | A kind of Ming River lily WRKY transcription factor genes LrWRKY1 and application | |
| JP2007507229A (en) | Method for enhancing stress tolerance in plants and method | |
| CA2680742A1 (en) | Improving cold- and salt-tolerant performance of plants with transcription factor gene snac2 from rice | |
| CN103421835B (en) | Application of transcription factor gene RrMYB11 in regulating and controlling rose types | |
| CN105624188B (en) | A kind of SPL18 gene is improving the application in plant products | |
| CN109384837B (en) | Poplar drought-resistant gene and application thereof | |
| CN110643618A (en) | Jatropha curcas MYB transcription factor JcMYB16 gene and application thereof in improving drought resistance of plants | |
| CN110938617A (en) | Lilium regale LrPAL-1 gene and application thereof | |
| CN101659699B (en) | Plant stress resistance-related protein GmSIK2 and coding gene and application thereof | |
| CN103172716B (en) | Heat-resistant plant gene and application thereof | |
| CN109750010B (en) | Salicornia europaea laccase and coding gene and application thereof | |
| CN109867715B (en) | Application of chloroplast protein and ATPase enzymatic activity mutant in improvement of stress resistance of plants | |
| CN115960189B (en) | Application of shinyleaf yellowhorn protein and encoding gene thereof in improving anthocyanin content in plant petals | |
| CN109354614B (en) | Application of OsCSLD4 protein in improving salt stress tolerance of plants | |
| CN103993019B (en) | The application of rose RrDFR1 gene in regulating plant anthocyanidin is synthetic | |
| CN101302523A (en) | Application of physiological clock regulation protein LHY to stress-tolerant plant cultivation | |
| CN103602688B (en) | Helianthus tuberosus L. Na<+>/H<+> reverse transport protein genes HtNHX1 and HtNHX2 and use thereof | |
| CN101883572A (en) | Sorghum aluminum tolerance gene, sbmate | |
| CN110862973B (en) | Rice thioredoxin gene OsNDU, protein, vector, host cell, molecular marking method and application | |
| CN114480414A (en) | Method for enhancing cold resistance of plants or cultivating plants with high cold resistance | |
| CN104673803B (en) | Application of gene methylation in regulation of gene expression | |
| CN104109682B (en) | A kind of pectin lyase BnPL gene and promoter thereof and application | |
| CN114085854A (en) | Rice drought-resistant and salt-tolerant gene OsSKL2 and application thereof | |
| CN108165555B (en) | Eggplant cultivation SmHQT gene core fragment, RNAi expression vector and application thereof | |
| CN107176983B (en) | Application of protein PpLEA3-3 in regulation and control of plant stress resistance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20191208 |