CN103417753A - Vegetable oil composition with heart and cerebral vessel protecting function, as well as preparation method and application of vegetable oil composition - Google Patents
Vegetable oil composition with heart and cerebral vessel protecting function, as well as preparation method and application of vegetable oil composition Download PDFInfo
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Abstract
The invention discloses a vegetable oil composition with a heart and cerebral vessel protecting function, as well as a preparation method of the vegetable oil composition. The vegetable oil composition comprises the following raw materials by weight: 1 to 30 parts of safflower seed oil, 5 to 50 parts of artemisia ordosica seed oil, 1 to 30 parts of spina date seed oil, 5 to 50 parts of purple perilla seed oil, 5 to 50 parts of linseed oil, 1 to 30 parts of grape seed oil and 1 to 20 parts of boxthorn seed oil. The vegetable oil composition is prepared by screening through a great amount of experiments, adopts natural traditional vegetable oil used as both medicine and food, has multiple health-care effects, is healthy and safe, and has no toxic and side effects; experiment results show that the vegetable oil composition can effectively regulate human blood fat, prevent arteriosclerosis, and soften blood vessels, has the heart and cerebral vessel protecting function, and can be extensively applied to hyperlipemia crowd caused by high cholesterol or high triglyceride.
Description
Technical field
The present invention relates to a kind of natural plants fluid composition, particularly relate to a kind of vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel and preparation method thereof.
Background technology
China's cardiovascular and cerebrovascular disease sickness rate, mortality rate and risk factor ascendant trend linearly still over nearly 10 years, and western countries have been mild downward trend.Cardiovascular and cerebrovascular disease forms the mortality rate (No.1 killer) of China 40.72%, for compatriots have caused huge health threat and financial burden.In the process of cardiovascular and cerebrovascular disease genesis, dyslipidemia is its major incentive and the form of expression.Dyslipidemia is commonly called as hyperlipidemia, refers to the disease that in blood, one or more lipid components increase extremely, and the concentration that main manifestations is the lipid compositions such as plasma cholesterol, triglyceride, total fat surpasses arm's length standard.The arteriosclerosis caused by hyperlipidemia is the pathologic basis that causes cardiovascular and cerebrovascular disease.So the arteriosclerosis that the control hyperlipidemia causes is the essential measure that the control cardiovascular and cerebrovascular disease occurs.
Prior art treatment hyperlipidemia mainly relies on blood lipid-lowering medicine and dietary adjustments.At present the clinical medicine that is usually used in blood fat reducing mainly contains fibrate (as fenofibrate, gemfibrozil, bezafibrate etc.), Statins (as lovastatin, simvastatin etc.), nicotinic acid class (as acipimox), and pantethine, clofibrate etc.But said medicine is in blood fat reducing, can causes blood sugar increasing, feel sick, the untoward reaction such as abdominal distention, diarrhoea and liver function injury.In recent years, domestic medical worker focuses on Chinese medicine preparation by the main target of blood lipid-lowering medicine exploitation, and has developed a plurality of products.Though the Reducing Blood Lipid by Chinese medicine goods show hypotoxicity, it belongs to medicine after all, can have side effects, and is not suitable for long-term taking, and the taste of Chinese medicine number too much can make treatment cost higher, and ordinary consumer is beyond affordability.Therefore, exploitation is made with medicine and food raw materials, can have no side effect by long-term taking, and has the nutraceutical of hypolipemic function, exists a large amount of market demands, also becomes the focus of those skilled in the art's research.
Summary of the invention
Goal of the invention: the object of the present invention is to provide the effect of a kind of protection cardiovascular and cerebrovascular vessel strong, safe, the vegetable oil composition had no adverse reaction.Another object of the present invention is to provide the preparation method and application of above-mentioned composition.
Technical scheme: in order to realize above purpose, the technical scheme that the present invention takes is:
A kind of vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel, it is made by the raw material of following parts by weight: safflower oil 1-30 part, 5～50 parts of sand sagebrush seed oils; 1～30 part of Semen Ziziphi Spinosae oil, 5～50 parts of Oleum Perillae, 5～50 parts of Semen Lini oil; 1～30 part of Oleum Vitis viniferae, 1～20 part of boxthorn seed oil.
As preferred version; the above-described vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel; it is made by the raw material of following parts by weight: safflower oil 5-20 part; 10～30 parts of sand sagebrush seed oils; 5～20 parts of Semen Ziziphi Spinosae oil, 8～25 parts of Oleum Perillae, 10～30 parts of Semen Lini oil; 5～20 parts of Oleum Vitis viniferaes, 3～10 parts of boxthorn seed oil.
As more preferably scheme, the described vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel, it is made by the raw material of following parts by weight: 10 parts of safflower oils; 25 parts of sand sagebrush seed oils, 10 parts of Semen Ziziphi Spinosae oil, 15 parts of Oleum Perillae; 25 parts of Semen Lini oil, 10 parts of Oleum Vitis viniferaes, 5 parts of boxthorn seed oil.
Preparation method with vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect of the present invention, it comprises the following steps:
(1) get Semen Flos Carthami through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain safflower oil;
(2) get the Folium et Cacumen Artemisiae Halodendri seed through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain sand sagebrush seed oil;
(3) get Semen Ziziphi Spinosae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Semen Ziziphi Spinosae oil;
(4) get Fructus Perillae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Oleum Perillae;
(5) get Semen Lini, through cold-press, expression separation obtains Semen Lini oil;
(6) get Semen Vitis viniferae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Oleum Vitis viniferae;
(7) get seed of Fructus Lycii through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain boxthorn seed oil;
(8) get respectively the safflower oil of step (1) gained by the described parts by weight of formula ratio, the sand sagebrush seed oil of step (2) gained, the Semen Ziziphi Spinosae oil of step (3) gained, the Oleum Perillae of step (4) gained, the Semen Lini oil of step (5) gained, the Oleum Vitis viniferae of step (6) gained, the boxthorn seed oil of step (7) gained, be placed in stainless cylinder of steel and mix and stir, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, the CO of step (1) safflower oil
2The condition of supercritical fluid extraction is: extracting pressure 40MPa, 50 ℃ of extraction temperature, extraction time 60min.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, step (2) sand sagebrush seed oil CO
2The condition of supercritical fluid extraction is: extracting pressure 42MPa, 48 ℃ of extraction temperature, extraction time 60min.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, step (3) Semen Ziziphi Spinosae oil CO
2The condition of supercritical fluid extraction is: extracting pressure 39MPa, 53 ℃ of extraction temperature, extraction time 60min.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, step (4) Oleum Perillae CO
2The condition of supercritical fluid extraction is: extracting pressure 41MPa, 52 ℃ of extraction temperature, extraction time 60min.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, step (6) Oleum Vitis viniferae CO
2The condition of supercritical fluid extraction is: extracting pressure 44MPa, 40 ℃ of extraction temperature, extraction time 60min.
As preferred version, the above has the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, step (7) boxthorn seed oil CO
2The condition of supercritical fluid extraction is: extracting pressure 40MPa, 51 ℃ of extraction temperature, extraction time 60min.
The present invention is according to the physicochemical property of active plant oil and the physicochemical property of other impurity in Semen Flos Carthami, Folium et Cacumen Artemisiae Halodendri seed, Semen Ziziphi Spinosae, Fructus Perillae, Semen Lini, Semen Vitis viniferae and seed of Fructus Lycii, screen extraction process by great many of experiments, wherein Semen Flos Carthami, Folium et Cacumen Artemisiae Halodendri seed, Semen Ziziphi Spinosae, Fructus Perillae, Semen Vitis viniferae and seed of Fructus Lycii all adopt the CO of optimization process parameter
2The supercritical fluid extraction method is extracted, and has an extraction efficiency high, and extraction temperature is low, does not need the steps such as conventional extracting method heating is concentrated, can not destroy active component in vegetable oil, and the advantage such as the impurity component dissolution rate is low.Semen Lini squeezes vegetable oil through cold-press, without heating, can guarantee that its active component is not destroyed.
Through lot of experiments, show; natural plants fluid composition provided by the invention can effectively reduce cholesterol, triglyceride and the low-density lipoprotein white level in hyperlipemia rat serum; improve hdl level; show that vegetable oil composition provided by the invention has obvious inhibitory action to the hyperlipidemia of rat, can be applicable to medicine or health product that preparation has the effect of protection cardiovascular and cerebrovascular vessel.As preferred scheme, the application of vegetable oil composition in preparation control hyperlipidemia disease and arteriosclerosis disease medicine with the effect of protection cardiovascular and cerebrovascular vessel provided by the invention.
Vegetable oil composition with protection cardiovascular and cerebrovascular vessel effect of the present invention prevents the application in the hyperlipidemia disease in preparation, vegetable oil composition and pharmaceutically acceptable carrier is made to the medicine of soft capsule, hard capsule, tablet, drop pill, pill, micropill, powder, syrup, mixture, soft extract or extractum dosage form.
When the vegetable oil composition with protection cardiovascular and cerebrovascular vessel effect provided by the invention is made soft capsule, vegetable oil composition directly or with mixing diluents evenly after, take gelatin, glycerol etc. is capsule casing material, uses encapsulating machine to be pressed into soft capsule.
When the vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel provided by the invention is made hard capsule; vegetable oil composition and lactose or corn starch mix homogeneously; or with lactose or corn starch, mix homogeneously again after the use cyclodextrin inclusion compound; through granulate, the encapsulated hard capsule of making then.
When the vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel provided by the invention is made tablet; vegetable oil composition and lactose or corn starch mix homogeneously; or with lactose or corn starch, mix homogeneously again after the use cyclodextrin inclusion compound; add magnesium stearate lubricant while needing, then tabletting is made tablet.
During the vegetable oil composition granulation agent with protection cardiovascular and cerebrovascular vessel effect provided by the invention; vegetable oil composition and lactose or corn starch mix homogeneously, or with lactose or corn starch, mix homogeneously again after using cyclodextrin inclusion compound, granulate; drying, the granulation agent.
When vegetable oil composition provided by the invention is made other dosage form, can prepare by the pharmacy conventional method.
Beneficial effect: the vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel provided by the invention; according to Chinese medicine health preserving, health care theory and modern pharmacological research achievement; screen and obtain by great many of experiments, take safflower oil, sand sagebrush seed oil, Semen Ziziphi Spinosae oil, Oleum Perillae, Semen Lini oil, Oleum Vitis viniferae and seed of Fructus Lycii wet goods medicinal and edible Chinese medicine to make as primary raw material.Wherein, safflower oil for separating through modern crafts the vegetable oil obtained from the medicinal and edible Chinese medicine Semen Carthami, sand sagebrush seed oil is the vegetable oil extracted through modern crafts from natural edible-plant Folium et Cacumen Artemisiae Halodendri seed, the two all is rich in unsaturated fatty acid, wherein the linoleic acid average content all can reach 80% left and right, and the two all goes back rich in vitamin E in addition.The effect that linoleic acid has blood fat reducing, removes blood vessel deposit and blood pressure lowering, and effect is obviously.Vitamin E has vessel softening, anti-aging effects, and the vitamin E in Semen Flos Carthami and sand sagebrush seed oil not only content is high, and its permeability is extremely strong, very easily is absorbed by the body.The two is worked in coordination with has lipid concentration in the blood of reduction, the atherosis effect of prevention of arterial.Semen Ziziphi Spinosae oil is the vegetable oil extracted through modern crafts from the medicinal and edible Chinese medicine Semen Ziziphi Spinosae, except containing a large amount of unsaturated fatty acids, still contain a large amount of Squalenes, reach the triterpene constituents, there is the raising hypoxia-bearing capability, promote conveying and the cell utilization of blood oxygen, contribute to allaying tiredness, the control headache, migraine, improve sleep, the effect of purifying the blood.Oleum Perillae be from Fructus Perillae through modern crafts separating obtained vegetable oil, its ɑ-linolenic acid content can reach 70%, is to find at present the vegetable oil that ɑ-linolenic acid content is the highest; Semen Lini oil is the vegetable oil extracted through modern crafts from the natural edible-plant Semen Lini, its main component is that ɑ-linolenic acid content can reach more than 60%, and contain and enrich the mineral elements such as flavonoid class composition and potassium, zinc, and prepare through cold-press, without heating, guaranteed that to greatest extent original composition is not destroyed.Modern medicine proof ɑ-linolenic acid, be the fatty acid of needed by human, and human body self can not synthesize, and it has multiple significant health care to human body, has the blood fat of adjusting, reduces the effects such as blood viscosity, vessel softening.In addition, alpha-linolenic acid can change into 20 carbon 5 olefin(e) acids (EPA) and 22 carbon 6 olefin(e) acids (DHA) after taking in human body under the effect of enzyme in human body, has the effect that improves memory.Oleum Vitis viniferae is the vegetable oil extracted through modern crafts from the natural edible-plant Semen Vitis viniferae, except containing a large amount of linoleic acids, also contains the abundant procyanidin constituents with antioxidant activity.Boxthorn seed oil is to extract through modern crafts the crude vegetal obtained from Chinese medicine Fructus Lycii seed, mainly contain a large amount of unsaturated fatty acids, comprise linoleic acid, alpha-linolenic acid, gamma-Linolenic acid, still contain in addition carotenoid, there is defying age, the vessel softening effect.The above natural traditional drugs food dual purpose plant oil with multiple health care is combined; can supplement simultaneously the multiple active component with cardiovascular and cerebrovascular vessel protective effect such as the necessary linoleic acid of human body, alpha-linolenic acid, gamma-Linolenic acid, vitamin E, flavonoid, procyanidin class and carotenoid, can be from the collaborative reduction blood fat that reaches of a plurality of links; the prevention of arterial sclerosis; the effects such as vessel softening, thus reach the effect of protecting cardiovascular and cerebrovascular vessel.The present invention screens the proportioning of each component by great many of experiments, scientific and reasonable; And whole preparation technology, automaticity is high, and preparation efficiency is high, can retain well the effective ingredient in raw material, does not bring any impurity into, has no side effect, and is applicable to the people with hyperlipidemia that hypercholesterolemia or high triglyceride cause.
The specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment only is not used in and limits the scope of the invention for the present invention is described, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
1 one kinds of preparation methoies with vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect of embodiment, comprise the steps:
(1) get Semen Flos Carthami, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 40MPa, 50 ℃ of extraction temperature, extraction time 60min, through separating to obtain safflower oil.
(2) get the Folium et Cacumen Artemisiae Halodendri seed, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 42MPa, 48 ℃ of extraction temperature, extraction time 60min, through separating to obtain sand sagebrush seed oil.
(3) get Semen Ziziphi Spinosae, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 39MPa, 53 ℃ of extraction temperature, extraction time 60min, through separating to obtain Semen Ziziphi Spinosae oil.
(4) get Fructus Perillae, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 41MPa, 52 ℃ of extraction temperature, extraction time 60min, through separating to obtain Oleum Perillae.
(5) get Semen Lini, through the standby Semen Lini oil of cool press for extracting juice legal system.
(6) get Semen Vitis viniferae, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 44MPa, 40 ℃ of extraction temperature, extraction time 60min, through separating to obtain Oleum Vitis viniferae.
(7) get seed of Fructus Lycii, pulverized 40 mesh sieves, charging, sealing, CO
2Supercritical fluid extraction, extracting pressure 40MPa, 51 ℃ of extraction temperature, extraction time 60min, through separating to obtain boxthorn seed oil.
(8) get respectively the safflower oil 100g of step (1) gained, the sand sagebrush seed oil 250g of step (2) gained, the Semen Ziziphi Spinosae oil 100g of step (3) gained, the Oleum Perillae 150g of step (4) gained, the Semen Lini oil 250g of step (5) gained, the Oleum Vitis viniferae 100g of step (6) gained, the boxthorn seed oil 50g of step (7) gained, be placed in stainless cylinder of steel and mix and stir, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain.
2 one kinds of vegetable oil compositions with the effect of protection cardiovascular and cerebrovascular vessel of embodiment
(1) treating excess syndrome is executed for example safflower oil 150g of step (1) gained respectively, the sand sagebrush seed oil 200g of step (2) gained, the Semen Ziziphi Spinosae oil 150g of step (3) gained, the Oleum Perillae 200g of step (4) gained, the Semen Lini oil 300g of step (5) gained, the Oleum Vitis viniferae 100g of step (6) gained, the boxthorn seed oil 100g of step (7) gained, being placed in stainless cylinder of steel mixes and stirs, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain the natural plants fluid composition.
(2) get the natural plants fluid composition made in step (1), take gelatin, glycerol etc. is capsule casing material, uses encapsulating machine to be pressed into soft capsule, every 0.5g.
3 one kinds of vegetable oil compositions with the effect of protection cardiovascular and cerebrovascular vessel of embodiment
(1) get respectively the safflower oil 50g of embodiment 1 step (1) gained, the sand sagebrush seed oil 300g of step (2) gained, the Semen Ziziphi Spinosae oil 50g of step (3) gained, the Oleum Perillae 200g of step (4) gained, the Semen Lini oil 300g of step (5) gained, the Oleum Vitis viniferae 50g of step (6) gained, the boxthorn seed oil 30g of step (7) gained, be placed in stainless cylinder of steel and mix and stir, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain.Obtain the natural plants fluid composition.
(2) get the natural plants fluid composition made in step (1), mix homogeneously with lactose again after using cyclodextrin inclusion compound, through granulate, the encapsulated hard capsule of making then.
4 one kinds of vegetable oil compositions with the effect of protection cardiovascular and cerebrovascular vessel of embodiment
(1) get respectively the safflower oil 100g of embodiment 1 step (1) gained, the sand sagebrush seed oil 250g of step (2) gained, the Semen Ziziphi Spinosae oil 100g of step (3) gained, the Oleum Perillae 150g of step (4) gained, the Semen Lini oil 250g of step (5) gained, the Oleum Vitis viniferae 100g of step (6) gained, the boxthorn seed oil 50g of step (7) gained, being placed in stainless cylinder of steel mixes and stirs, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain the natural plants fluid composition.
(2) get the natural plants fluid composition made in step (1), take gelatin, glycerol etc. is capsule casing material, uses encapsulating machine to be pressed into soft capsule, every 0.5g.
5 one kinds of vegetable oil compositions with the effect of protection cardiovascular and cerebrovascular vessel of embodiment
(1) get respectively the safflower oil 200g of embodiment 1 step (1) gained, the sand sagebrush seed oil 100g of step (2) gained, the Semen Ziziphi Spinosae oil 50g of step (3) gained, the Oleum Perillae 250g of step (4) gained, the Semen Lini oil 300g of step (5) gained, the Oleum Vitis viniferae 200g of step (6) gained, the boxthorn seed oil 50g of step (7) gained, being placed in stainless cylinder of steel mixes and stirs, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain the natural plants fluid composition.
(2) get natural plants fluid composition lactose, the starch mix homogeneously made in step (1), add magnesium stearate lubricant, then tabletting is made tablet.
6 one kinds of vegetable oil compositions with the effect of protection cardiovascular and cerebrovascular vessel of embodiment
Get respectively the safflower oil 150g of embodiment 1 step (1) gained, the sand sagebrush seed oil 200g of step (2) gained, the Semen Ziziphi Spinosae oil 100g of step (3) gained, the Oleum Perillae 200g of step (4) gained, the Semen Lini oil 150g of step (5) gained, the Oleum Vitis viniferae 50g of step (6) gained, the boxthorn seed oil 50g of step (7) gained, being placed in stainless cylinder of steel mixes and stirs, mixed oil liquid grinds 2～3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain the natural plants fluid composition.
Embodiment 7 lipid-lowering test researchs
One, experiment material and medicine
1. medicine and reagent
T-CHOL (TC) is measured test kit, and triglyceride (TG) is measured test kit, and HDL-C (HDL-C) is measured test kit, all purchased from Nanjing, builds up bio-engineering research institute.JIANGZHINING KELI (Luoyang Junshan Pharmaceutical Co., Ltd.'s production).High lipid food formula: cholesterol 4%, Adeps Sus domestica 10%, methylthiouracil 0.2%, cholate 0.5%%, normal feedstuff 85%.
2. experimental apparatus
Enzyme linked immunological instrument (U.S. Bio-Tek company); UV-2000 type ultraviolet spectrophotometer (Beijing Lai Baitaike instrument company limited); BT125 type electronic balance (Sai Duolisi scientific instrument company limited); KQ-250E type ultrasonic cleaner (Kunshan standing grain wound ultrasonic instrument company limited); AnkeGL-16GII type centrifuge (Anting Scientific Instrument Factory, Shanghai).
3. laboratory animal
Clean level male ICR mouse, body weight 18～22g, provided by Zhejiang Province's Experimental Animal Center, quality certification SCXK(Zhejiang) 2008-0033.
Two, experimental technique
1. the foundation of high blood lipid model
After the healthy mice adaptability is fed to 3d, random packet, be respectively blank group, hyperlipidemia model group, positive controls and the basic, normal, high concentration dose group of tested vegetable oil composition, and 8 every group, to weigh and keep a record, each organizes the different not statistically significant of the mesosome method of double differences.Experiment mice is except the blank group, and all the other respectively organize the feeding high lipid food to induce the formation of hyperlipidemia.After 20d, respectively extract 4 mices from blank group and high fat group at random, pluck eyeball and get hematometry T-CHOL (TC), triglyceride (TG) and HDL-C (HDL-C) level, to determine the foundation of hyperlipidemia model.
2. hyperlipidemia experimental technique
The common Mus grain of blank group feeding, the mice of built vertical hyperlipidemia continues the feeding high lipid food, and animal freely ingests and drinks water.The high, medium and low dosage group of vegetable oil composition every day that the embodiment of the present invention 1 and embodiment 4 are prepared is respectively with 16g/kg, 8g/kg, 4g/kg, the corresponding tested material of gavage, blank group and hyperlipidemia model group gavage equivalent distilled water, the positive drug group is with the JIANGZHINING KELI gavage of 0.25g/kg, and morning every day, gavage was 1 time.Continuous gavage 20d, rear fasting 24h, pluck eyeball and get blood and liver after weighing, measure the concentration of TC, TG and HDL-C indices according to reagent packed meal description.
3. statistical procedures
All experimental datas all adopt the SPSS11.5 statistical processing software to carry out statistical procedures, result with
Mean, group difference adopts the t check, and there is statistical significance P<0.05 for difference.
Three, experimental result
1. blood fat reducing result of the test
After high lipid food is fed mice 20d, the variation of lipid of mice level is measured in sampling, and concrete outcome is in Table 1.Mice is after high lipid food is fed mice 20d as can be seen from Table 1, and mice serum TC, TG content significantly raise (P<0.01), and HDL-C content significantly reduces (P<0.01), shows that the hyperlipemia in mice model is successfully established.
After the vegetable oil composition that mice prepares to respectively positive drug and embodiment 1 and embodiment 4, measure the content of TC, TG and HLD-C in its serum.Result is as shown in table 2, high lipid food group and blank group are relatively, nursing through 40d, pathological model group mice serum TC and TG content are all apparently higher than blank group (P<0.01), HLD-C content all is starkly lower than blank group (P<0.01), illustrates that the hyperlipidemia animal model in this experiment is successfully.Compare with high fat pathological model group, give embodiment 1 and the embodiment high, medium and low dosage group of 4 gained vegetable oil composition and positive controls and all reduced mice serum TC and TG content, HLD-C content has raise.Wherein combine object height, middle dosage group is compared with the hyperlipidemia model group, TC, TG and HLD-C level difference have statistical significance (P<0.05), show that vegetable oil composition provided by the present invention has good effect for reducing blood fat.
The foundation of table 1 hyperlipemia mice model
| Group | Number of animals | TC | TG | HDL-C |
| The blank group | 4 | 2.23±0.19 | 1.06±0.14 | 2.11±0.39 |
| The hyperlipidemia model group | 4 | 4.49±0.57 ## | 2.80±0.42 ## | 1.24±0.15 ## |
Annotate:
##P<0.01, mean to compare with the blank group.
The impact of table 2 vegetable oil composition on high blood lipid model mice TC, TG and HDL-C content
| Group | Dosage (g/kg) | TC(mmol/L) | TG(mmol/L) | HDL-C(mmol/L) |
| The blank group | 0 | 2.873±0.34 | 1.25±0.22 | 2.13±0.25 |
| The hyperlipidemia model group | 0 | 4.63±0.38 ## | 2.78±0.44 ## | 1.53±0.19 ## |
| The positive drug group | 0.25 | 3.69±0.39** | 1.69±0.27** | 2.01±0.33** |
| Embodiment 1 low dose group | 4 | 4.32±0.41* | 2.36±0.45 | 1.62±0.21 |
| Dosage group in embodiment 1 | 8 | 4.01±0.45** | 2.25±0.12* | 1.80±0.17* |
| Embodiment 1 high dose group | 16 | 3.71±0.34** | 1.93±0.15** | 1.91±0.20* |
| Embodiment 4 low dose group | 4 | 4.42±0.25* | 2.51±0.31 | 1.60±0.18 |
| Dosage group in embodiment 4 | 8 | 4.13±0.31** | 2.27±0.17* | 1.82±0.17* |
| Embodiment 4 high dose group | 16 | 3.92±0.38** | 1.82±0.19** | 1.87±0.15* |
Annotate:
##P<0.01, mean to compare with the blank group; *: P<0.05, * * P<0.01, mean to compare with model group.
2. the calculating of atherogenic index (AI)
Atherogenic index (AI) can reflect the distribution situation of lipoprotein cholesterol in animal or human's body, and when the AI value increases, atherosclerosis is dangerous just to be increased.
AI=(TC﹣HDL-C)/HDL-C
Modern study proves, high TC and LDL-C, and low HDL-C is atherosclerotic important risk factor.Therefore, at atherogenic index AI commonly used medically, mean atherosclerotic danger, atherosclerotic probability increase occurs in the AI value prompting body that raises.
As can be seen from Table 3, the AI value of each group is starkly lower than high fat contrast class value, all reaches significant level (P<0.05).Show that vegetable oil composition provided by the present invention has good preventive effect to atherosclerosis.
The impact of table 3 vegetable oil composition on high blood lipid model mice atherogenic index (AI)
| Group | Dosage (g/kg) | Atherogenic index (AI) |
| The blank group | 0 | 0.38±0.12 |
| The hyperlipidemia model group | 0 | 2.13±0.35 ## |
| The positive drug group | 0.25 | 0.88±0.24** |
| Embodiment 1 low dose group | 4 | 1.59±0.31* |
| Dosage group in embodiment 1 | 8 | 1.35±0.30** |
| Embodiment 1 high dose group | 16 | 1.15±0.18** |
| Embodiment 4 low dose group | 4 | 1.73±0.20* |
| Dosage group in embodiment 4 | 8 | 1.48±0.29** |
| Embodiment 4 high dose group | 16 | 1.21±0.11** |
Annotate:
##P<0.01, mean to compare with the blank group; *: P<0.05, * * P<0.01, mean to compare with model group.
Above experimental result shows; natural plants fluid composition provided by the invention can effectively reduce T-CHOL and the triglyceride levels of high blood lipid model mice; the high density lipoprotein increasing cholesterol levels; and can reduce atherogenic index; show that natural plants fluid composition provided by the invention has good effect for reducing blood fat, can reach the effect of protection cardiovascular and cerebrovascular vessel.And with existing medicine, compare, said composition be take the natural traditional drugs food dual purpose plant oil with multiple health care and is raw material, safer, has no adverse reaction.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. one kind has the vegetable oil composition of protecting the cardiovascular and cerebrovascular vessel effect; it is characterized in that; it is made by the raw material of following parts by weight: safflower oil 1-30 part; 5 ~ 50 parts of sand sagebrush seed oils; 1 ~ 30 part of Semen Ziziphi Spinosae oil, 5 ~ 50 parts of Oleum Perillae, 5 ~ 50 parts of Semen Lini oil; 1 ~ 30 part of Oleum Vitis viniferae, 1 ~ 20 part of boxthorn seed oil.
2. the vegetable oil composition with protection cardiovascular and cerebrovascular vessel effect according to claim 1; it is characterized in that; it is made by the raw material of following parts by weight: safflower oil 5-20 part; 10 ~ 30 parts of sand sagebrush seed oils; 5 ~ 20 parts of Semen Ziziphi Spinosae oil, 8 ~ 25 parts of Oleum Perillae, 10 ~ 30 parts of Semen Lini oil; 5 ~ 20 parts of Oleum Vitis viniferaes, 3 ~ 10 parts of boxthorn seed oil.
3. the vegetable oil composition with protection cardiovascular and cerebrovascular vessel effect according to claim 2; it is characterized in that; it is made by the raw material of following parts by weight: 10 parts of safflower oils; 25 parts of sand sagebrush seed oils; 10 parts of Semen Ziziphi Spinosae oil, 15 parts of Oleum Perillae, 25 parts of Semen Lini oil; 10 parts of Oleum Vitis viniferaes, 5 parts of boxthorn seed oil.
4. the described preparation method with vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect of claim 1 ~ 3 any one, is characterized in that, comprises the following steps:
(1) get Semen Flos Carthami through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain safflower oil;
(2) get the Folium et Cacumen Artemisiae Halodendri seed through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain sand sagebrush seed oil;
(3) get Semen Ziziphi Spinosae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Semen Ziziphi Spinosae oil;
(4) get Fructus Perillae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Oleum Perillae;
(5) get Semen Lini, through cold-press, expression separation obtains Semen Lini oil;
(6) get Semen Vitis viniferae through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain Oleum Vitis viniferae;
(7) get seed of Fructus Lycii through vacuum outgas, then adopt CO
2Supercritical fluid extraction, separate and obtain boxthorn seed oil;
(8)) get respectively the safflower oil of step (1) gained by the described parts by weight of claims 1 to 3, the sand sagebrush seed oil of step (2) gained, the Semen Ziziphi Spinosae oil of step (3) gained, the Oleum Perillae of step (4) gained, the Semen Lini oil of step (5) gained, the Oleum Vitis viniferae of step (6) gained, the boxthorn seed oil of step (7) gained, be placed in stainless cylinder of steel and mix and stir, mixed oil liquid grinds 2 ~ 3 times through colloid mill, make to be mixed into uniform liquid, be down to room temperature, obtain.
5. there is according to claim 4 the preparation method of the vegetable oil composition of protection cardiovascular and cerebrovascular vessel effect, it is characterized in that step (1) CO
2The condition of supercritical fluid extraction is: extracting pressure 40MPa, 50 ° of C of extraction temperature, extraction time 60 min; Step (2) CO
2The condition of supercritical fluid extraction is: extracting pressure 42MPa, 48 ° of C of extraction temperature, extraction time 60 min; Step (3) CO
2The condition of supercritical fluid extraction is: extracting pressure 39MPa, 53 ° of C of extraction temperature, extraction time 60 min; Step (4) CO
2The condition of supercritical fluid extraction is: extracting pressure 41MPa, 52 ° of C of extraction temperature, extraction time 60 min; Step (6) CO
2The condition of supercritical fluid extraction is: extracting pressure 44MPa, 40 ° of C of extraction temperature, extraction time 60 min; Step (7) CO
2The condition of supercritical fluid extraction is: extracting pressure 40MPa, 51 ° of C of extraction temperature, extraction time 60 min.
6. the described application of vegetable oil composition in preparation control hyperlipidemia disease and arteriosclerosis disease medicine with the effect of protection cardiovascular and cerebrovascular vessel of claims 1 to 3.
7. the vegetable oil composition with the effect of protection cardiovascular and cerebrovascular vessel according to claim 6 is prevented and treated the application in the high fat of blood disease drug in preparation; it is characterized in that, vegetable oil composition and pharmaceutically acceptable carrier are made to the medicine of soft capsule, hard capsule, tablet, drop pill, pill, micropill, powder, syrup, mixture, soft extract or extractum dosage form.
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| CN105232802A (en) * | 2015-11-05 | 2016-01-13 | 南京中医药大学 | Procyanidine composition with blood fat reducing function and preparation method and application thereof |
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| CN103931797A (en) * | 2014-04-11 | 2014-07-23 | 宁夏吴忠市国海粮油有限公司 | Nutritional health-care flax blend oil |
| CN104207295A (en) * | 2014-09-30 | 2014-12-17 | 秦富英 | Wild jujube seed oil compound antioxidant |
| CN104207295B (en) * | 2014-09-30 | 2016-04-13 | 桂林融通科技有限公司 | A kind of spine date seed oil composite antioxidant |
| CN105708897A (en) * | 2014-12-01 | 2016-06-29 | 李玉明 | Applications of omega-3 aliphatic acid-containing plant seeds in drug preparation, health care products, and functional food with health care effect, and preparation method |
| CN105232802A (en) * | 2015-11-05 | 2016-01-13 | 南京中医药大学 | Procyanidine composition with blood fat reducing function and preparation method and application thereof |
| CN105232802B (en) * | 2015-11-05 | 2020-02-14 | 南京中医药大学 | Procyanidine composition with blood fat reducing effect and preparation method and application thereof |
| CN105617038A (en) * | 2016-02-01 | 2016-06-01 | 云南一尧科技开发有限公司 | Rhizoma gastrodiae and Ganoderma lucidum composition with healthcare function |
| CN107149629A (en) * | 2017-05-15 | 2017-09-12 | 中国科学院西北高原生物研究所 | A kind of matrimony vine fat capsule with anti-oxidation efficacy and preparation method thereof |
| CN109420156A (en) * | 2017-08-26 | 2019-03-05 | 杨振峰 | A kind of drug of thrombolysis lipid-lowering row bolt |
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Application publication date: 20131204 |