CN103417685A - Traditional Chinese medicine granules for controlling gill-rot diseases of prawns and preparation method thereof - Google Patents
Traditional Chinese medicine granules for controlling gill-rot diseases of prawns and preparation method thereof Download PDFInfo
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Abstract
The invention discloses traditional Chinese medicine granules for controlling gill-rot diseases of prawns and a preparation method of the traditional Chinese medicine granules. The traditional Chinese medicine granules are composed of Chinese pulsatilla roots, rheum officinale, radix scutellariae, cape jasmine, kalmegh and radix glycyrrhizae. The traditional Chinese medicine granules comprise the following components, by weight, 3-6 parts of Chinese pulsatilla roots, 2-4 parts of rheum officinale, 1-2 parts of radix scutellariae, 1-2 parts of cape jasmine, 1-2 parts of kalmegh and 1-2 parts of radix glycyrrhizae. The traditional Chinese medicine granules are prepared by conducting decoction and then adding cane sugar and starch. The traditional Chinese medicine materials are reasonably combined in the traditional Chinese medicine granules for controlling the gill-rot diseases of the prawns, therefore, the function of controlling the gill-rot diseases of the prawns can be achieved, the traditional Chinese medicine granules capable of controlling the gill-rot diseases of the prawns are provided for the aquaculture industry, the traditional Chinese medicine granules have the advantages of being rapid in effectiveness, definite in curative effect and free of medicine residues and any toxic and side effect, and the preparation method is simple and capable of meeting the requirements for large-scale industrial production.
Description
Technical field
The present invention relates to a kind of Chinese medicine granules and preparation method thereof, particularly relate to a kind of Chinese medicine granules of preventing and treating gill and preparation method thereof.
Background technology
Cause of disease is vibrio, pseudomonas and Aeromonas.The ill shrimp gill filament is canescence, swelling, becomes fragile, and then from tip, to base portion, festers.The downright bad part of festering can cause prawn death.The gill rot prawn seldom survives.Aquatic medicine is controlled stricter, and Western medicine abuse of antibiotics situation is serious, and the disease treatment difficulty strengthens, and the drug residue problem is more and more heavier, threatens to the food safety problem.Primary disease there is no effective Therapeutic Method at present.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine granules of preventing and treating gill.
Another technical problem that the present invention will solve is to provide the preparation method of the Chinese medicine granules of above-mentioned control gill.
For realizing that the technical scheme that purpose of the present invention adopts is:
A kind of Chinese medicine granules of preventing and treating gill, the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, consist of, it counts 3～6 parts of the Radix Pulsatillaes, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, 1～2 part, Radix Glycyrrhizae by weight.
Preferably, the Chinese medicine granules of above-mentioned control gill is obtained by following method:
(1) taking by weight 1～2 part of 3～6 parts of the Radix Pulsatillae, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, Radix Glycyrrhizae is raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Preferably, the Chinese medicine granules of above-mentioned control gill, by 4～6 parts of its parts by weight Radix Pulsatillaes, 3～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, 1～2 part, Radix Glycyrrhizae.
Preferably, the Chinese medicine granules of above-mentioned control gill, by 5 parts of its parts by weight Radix Pulsatillaes, 4 parts of Radix Et Rhizoma Rhei, 2 parts of Radix Scutellariaes, 2 parts of Fructus Gardeniaes, 2 parts of Herba Andrographis, 2 parts, Radix Glycyrrhizae.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) taking by weight 1～2 part of 3～6 parts of the Radix Pulsatillae, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, Radix Glycyrrhizae is raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Compared with prior art, the invention has the beneficial effects as follows:
The Chinese medicine granules of above-mentioned control gill, by the Chinese medicine reasonable formula, make up the shortage of existing medicine, the single defect of preparation formulation, function with control gill, for providing a kind of, culture fishery can prevent and treat Chinese medicine granules of gill and preparation method thereof, determined curative effect, and no drug residue, problem has no side effect, has no drug resistance; Its preparation method is simple, is applicable to the needs that large-scale industrial is produced.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 3kg, Radix Et Rhizoma Rhei 2kg, Radix Scutellariae 1kg, Fructus Gardeniae 1kg, Herba Andrographis 1kg, Radix Glycyrrhizae 1kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 3kg, Radix Et Rhizoma Rhei 2kg, Radix Scutellariae 1kg, Fructus Gardeniae 1kg, Herba Andrographis 1kg, Radix Glycyrrhizae 1kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Embodiment 2
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 2kg, Fructus Gardeniae 2kg, Herba Andrographis 2kg, Radix Glycyrrhizae 2kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 2kg, Fructus Gardeniae 2kg, Herba Andrographis 2kg, Radix Glycyrrhizae 2kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Embodiment 3
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 4kg, Radix Et Rhizoma Rhei 3kg, Radix Scutellariae 1kg, Fructus Gardeniae 1kg, Herba Andrographis 1kg, Radix Glycyrrhizae 1kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 4kg, Radix Et Rhizoma Rhei 3kg, Radix Scutellariae 1kg, Fructus Gardeniae 1kg, Herba Andrographis 1kg, Radix Glycyrrhizae 1kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Embodiment 4
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 3kg, Radix Scutellariae 1kg, Fructus Gardeniae 2kg, Herba Andrographis 1kg, Radix Glycyrrhizae 2kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 3kg, Radix Scutellariae 1kg, Fructus Gardeniae 2kg, Herba Andrographis 1kg, Radix Glycyrrhizae 2kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Embodiment 5
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 6kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 2kg, Fructus Gardeniae 1kg, Herba Andrographis 2kg, Radix Glycyrrhizae 1kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 6kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 2kg, Fructus Gardeniae 1kg, Herba Andrographis 2kg, Radix Glycyrrhizae 1kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
Embodiment 6
A kind of Chinese medicine granules of preventing and treating gill, be comprised of the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, wherein Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 1kg, Fructus Gardeniae 2kg, Herba Andrographis 1kg, Radix Glycyrrhizae 2kg.
The preparation method of the Chinese medicine granules of above-mentioned control gill, concrete preparation process is as follows:
(1) take Radix Pulsatillae 5kg, Radix Et Rhizoma Rhei 4kg, Radix Scutellariae 1kg, Fructus Gardeniae 2kg, Herba Andrographis 1kg, Radix Glycyrrhizae 2kg are raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
The described Chinese crude drug of one of embodiment 1-6 is commercially available prod, and each amounts of components is increased or reduces according to same ratio, and the parts by weight relation of each component of gained all belongs to protection scope of the present invention.
The described Chinese crude drug of embodiment 1-6 all meets " Chinese veterinary pharmacopoeia " version two ministerial standards in 2010.
The composition principle of Chinese medicine granules of the present invention is: Radix Pulsatillae heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery; Radix Et Rhizoma Rhei purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body; Radix Scutellariae heat clearing and damp drying, eliminating fire and detoxication; Fructus Gardeniae eliminating fire and detoxication, clearing away heat and promoting diuresis; Herba Andrographis laryngopharynx swelling and pain, damp-heat diarrhea; Radix Glycyrrhizae cushion toxicity, strong.All medicines share, and mutual Synergistic has the effect of anti-inflammation, enhancing immunity, remarkably productive to the control gill, especially adopts the described proportioning of embodiment 2, and the effect for the treatment of gill is very remarkable, particularly is applicable to the treatment of control gill.
The clinical trial example:
The usage of the Chinese medicine granules of above-mentioned control gill is: the ratio in 10～15g/100kg body weight is mixed pharmaceutical chemistry, makes prawn oral, is used in conjunction 3～5 days.Reach the purpose for the treatment of disease after oral by intestinal absorption.
On August 7th, 2011,200 mu of Chinese prawn morbidities that raise at animal Community in Zhanjiang, Guangdong Culture of Penaeus Chinensis family, clinical symptoms: sick shrimp is bubbled through the water column, moves about slowly, bradykinesia, anorexia, the visible gill filament of microscopy festers, dead rapidly.Through pathogen identification, it is vibrio.Then use embodiment 2 Drug therapys, in the ratio of 15g/100kg body weight, mix pharmaceutical chemistry, make prawn oral, medication is after 5 hours, and the state of an illness is clearly better, and moves about and recovers to some extent, and appetite increases, and mortality rate is by a day reduction, and after 3 days, mortality rate is 0, and sick shrimp is almost recovered.
Above-mentioned detailed description of this kind of Chinese medicine granules of preventing and treating gill and preparation method thereof being carried out with reference to embodiment; illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under general plotting of the present invention, within should belonging to protection scope of the present invention.
Claims (5)
1. a Chinese medicine granules of preventing and treating gill, it is characterized in that: the Radix Pulsatillae, Radix Et Rhizoma Rhei, Radix Scutellariae, Fructus Gardeniae, Herba Andrographis and Radix Glycyrrhizae, consist of, it counts 3～6 parts of the Radix Pulsatillaes, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, 1～2 part, Radix Glycyrrhizae by weight.
2. the Chinese medicine granules of control gill according to claim 1 is characterized in that: by following method, obtained:
(1) taking by weight 1～2 part of 3～6 parts of the Radix Pulsatillae, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, Radix Glycyrrhizae is raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
3. the Chinese medicine granules of control gill according to claim 1 and 2, is characterized in that: by 4～6 parts of its parts by weight Radix Pulsatillaes, 3～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, 1～2 part, Radix Glycyrrhizae.
4. the Chinese medicine granules of control gill according to claim 1 and 2, is characterized in that: by 5 parts of its parts by weight Radix Pulsatillaes, 4 parts of Radix Et Rhizoma Rhei, 2 parts of Radix Scutellariaes, 2 parts of Fructus Gardeniaes, 2 parts of Herba Andrographis, 2 parts, Radix Glycyrrhizae.
5. the preparation method of the Chinese medicine granules of control gill claimed in claim 1 is characterized in that: concrete preparation process is as follows:
(1) taking by weight 1～2 part of 3～6 parts of the Radix Pulsatillae, 2～4 parts of Radix Et Rhizoma Rhei, 1～2 part of Radix Scutellariae, 1～2 part of Fructus Gardeniae, 1～2 part of Herba Andrographis, Radix Glycyrrhizae is raw material;
(2) to the water that adds 4 times of amounts of medical material gross weight in above-mentioned Six-element Chinese medicine, decoct 2 times, decoct for the first time 2 hours, decoct for the second time 100 minutes, collecting decoction, filter, and gets supernatant standing 12 hours, and supernatant is obtained to concentrated extract under 80 ℃ of conditions;
(3) step (2) gained concentrated extract is added to dehydrated alcohol to containing alcohol, measuring 60%(v/v), standing 12 hours, filter, under 70 ℃ of conditions, filtrate is concentrated into to relative density 1.38, and recovery ethanol, count by weight every part and add 2 portions of sucrose, 3 parts of starch mix homogeneously after concentrated in filtrate, with 85%(v/v) ethanol granulation cross 10 mesh sieves, 60 ℃ of oven dry, after 10 mesh sieves and get final product.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257812A (en) * | 2014-09-29 | 2015-01-07 | 浙江海洋学院 | Fish medicine for treating branchiomycosis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257812A (en) * | 2014-09-29 | 2015-01-07 | 浙江海洋学院 | Fish medicine for treating branchiomycosis |
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Application publication date: 20131204 |