CN103416208B - Cultivation method of cold-resistant palms - Google Patents
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Abstract
The invention relates to the technical field of seedling cultivation, in particular to a cultivation method of cold-resistant palms, which is characterized by comprising the steps of seed collection, seed selection, seed cold-resistant treatment, pregermination, sowing, seedling management, cold acclimation and cold-resistant palm haploid breeding. The method has the beneficial effects that the seedling emergence rate of palm seeds during planting in the processes of seed collection and seed selection is ensured; the stability of cell membranes of the palm seeds and the activity of antioxidant enzyme in cells are improved by performing cold-resistant treatment on the palm seeds, so that the cold resistance of palm seedlings erupted after the palm seeds are germinated is improved; due to the adoption of the cold acclimation process, the palm seedlings gradually obtain better cold-resistant exercise, the palm seedlings with better cold-resistant ability are screened out, a foundation is provided for the cultivation of cold-resistant palms with trunks and leaves which are thicker and stronger than those of common palms, the ability of resisting external severe environment is enhanced, and the draught resistance ability is also improved while the cold resistance is enhanced.
Description
Technical field
The present invention relates to stock growing technical field, particularly the breeding method of a kind of cold-resistant palm.
Background technology
Palm (Trachycarpusfortune) originates in China, and existing all have cultivation all over the world, is one of the most cold-resistant in the world Palmae (Palmae) plant.Palm is mainly distributed in the Qinling Mountains in China, on the south the Yangtze river basin warm and moist area of heavy rainfull, and maximum with Sichuan, Yunnan, Guizhou, Hunan, Hubei, Shaanxi, vertical distribution is 300～1500 meters of height above sea level, and southwest can reach 2700 meters.The weather of palm property happiness warm and moist, extremely cold-resistant, more resistance to the moon, becomes grade drought-enduring, only can not support and be subject to the too large day and night temperature difference.
Cultivating soil requires draining good, fertile.Palm root system is more shallow, without main root, should not be excessively dark when kind, and after planting, cave face will keep plate shape.Palm is to stronger resistances of multiple pernicious gas tool such as flue dust, sulphur dioxide, hydrogen fluoride, and has absorbing capacity, is suitable for air shed establishing in large scale.In addition, palm also has many purposes, and the densification of trunk texture is soft, moisture resistance anticorrosion in outer heavily fortified point, is good building materials; Leaf sheath fiber can broom processed, hairbrush, straw rain cape, bolster, mattress, water tower screen pack etc.; Palm fibre micromicro rope making rope; Palm leaf can be used as rain-proof shelter lid; Flower, really, brown root and phyllopodium Petiolus Trachycarpi can process and be used as medicine, and cures mainly the various diseases such as metal-inflicted wound, mange, band collapse, have blood in stool, dysentery; Seed wax coating for a big pill can extract the high melting-point wax of industrial use; Plant benevolence and contain abundant starch and protein, after abrasive dust, can make animal feeding-stuff; It is edible that the petal of not blooming also can be done vegetables.
Dropping in production over a large area and the phenomenon such as dead usually appears that cold resistance is poor or causes in palm " south plant north draw " or breeding cultivation.
In view of foregoing description, urgently there is the appearance of the breeding method of a kind of cold-resistant palm, the cold resistance of common palm is promoted greatly, thereby can make palm extensively be planted in the land of Beijing area.
Summary of the invention
The object of this invention is to provide the breeding method of a kind of cold-resistant palm, thereby can make cold-resistant palm extensively be planted in the land of Beijing area.
For solving the problems of the technologies described above, the present invention realizes by the following technical solutions.
A breeding method for cold-resistant palm, is characterized in that, comprises the following steps:
1) collect seed: while selecting wild cold-resistant palm to start to have seed shedding, start to collect seed, reject near the described cold-resistant palm fruit that has granule worm excrement the outside and carpopodium of cold-resistant palm fruit, pluck intact described cold-resistant palm fruit, for subsequent use;
2) seed selection: will be described after collecting seed cold-resistant palm fruit immersion 36～48h, by the cold-resistant palm fruit emerging drag for need not, select good described cold-resistant palm fruit the threshing of being dried in the shade of sinking to obtain cold-resistant palm seed;
3) the cold-resistant processing of seed: be first that 0.2～0.5% potassium permanganate is to described cold-resistant palm seed disinfection 15～20min with mass percent concentration, sterilization is rear with cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, the magnesium chloride solution that described cold-resistant palm seed is placed in to 30～40mg/L soaks 12～18h, afterwards described cold-resistant palm seed sand is buried to 36～48h temperature and remain on 35～45 DEG C, finally use cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, for subsequent use;
4) vernalization: before sowing,, with the water seed soaking 2～3h of 40～50 DEG C, the water seed soaking 20～24h of 20～30 DEG C, pulls described cold-resistant palm seed out, moisture dries in the shade, soak 10min with 3～5% glycerites afterwards, pull the described cold-resistant palm seed moisture that dries in the shade out, for subsequent use;
5) sowing: first assorted soil excrement, yellow ground and cultivation Soil tillage were evenly made to Nutrition Soil before sowing after, nitrolim is evenly spread fertilizer over the fields on described Nutrition Soil and carries out sterilization treatment to described Nutrition Soil, described Nutrition Soil after sterilizing fertilising is installed in plant planting bag, afterwards 2～3, described cold-resistant palm seed after treatment described vernalization is seeded in 1 described plant planting bag, on described cold-resistant palm seed, thickness of earth covering is 18～22cm;
6) seedling management: after emerging, watered once every 1～2 day, sprayed 5～7%666 liquids every 2～3 weeks, after emerging one month, the described plant planting bag of not emerging is removed, by cold-resistant palm seedling splitting plantation in the described planting bag of 2～3 of emerging;
7) domestication by low temperature: in the time that cold-resistant palm seedling grows tall 30～50cm, described cold-resistant palm seedling is planted and cultivated in canopy soil, spacing in the rows is 0.8～1.0m, and described cultivation temperature of shed is adjusted into 4～15 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-5～10 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-15～0 DEG C, cultivate six months;
8) cold-resistant palm haploid breeding: the described cold-resistant palm seedling that described domestication by low temperature is finished to rear survival was transplanted in natural environment in spring, grow up to after ripe cold-resistant palm until described cold-resistant palm seedling, after staminiferous plant in described cold-resistant palm blooms, gathering its pollen and carry out vitro anther culture and obtain monoploid seedling, is that 0.4～0.6% colchicine obtains palm seedling and is purebred described cold-resistant palm to spraying mass percent concentration on described monoploid seedling; Female plant in described cold-resistant palm carries out Plant Tissue Breeding and obtains a large amount of described cold-resistant palms.
Preferably, described step 2) described cold-resistant palm fruit immersion 48h.
Preferably, described step 3) described cold-resistant palm seed is placed in the magnesium chloride solution of 35mg/L.
Preferably, described step 4) sow the water seed soaking 2h with 45 DEG C before described cold-resistant palm seed.
Preferably, described step 5) on cold-resistant palm seed, thickness of earth covering is 20cm described in when sowing.
Preferably, described step 5) soil assorted excrement, described yellow ground and described cultivation soil volume ratio 1～2:5～7:12～13 described in Nutrition Soil described in when sowing.
Preferably, described step 5) plant planting bag specification is 15 × 20cm described in when sowing.
Preferably, described step 8) described colchicine concentration is 0.4～0.6%.
Vitro anther culture is a kind of tissue culture technique, and its process is: 1. pollen development to the flower pesticide of certain phase, by aseptic technique, be seeded in and on synthetic medium, carry out cultured in vitro; 2. under the specified conditions that pollen provides at medium, can occur repeatedly to divide, form similar embryo's structure (embryoid) or callus; 3. evoked callus differentiation is sprouted and root, finally grows up to plant.
Haploid breeding is a kind of breeding method, and its process is: on the basis of vitro anther culture, continue to process monoploid seedling with colchicine, chromosome number is doubled, again revert to diploidy number.Because their diploidy number chromosome itself is doubled by haploidy number chromosome, so be all pure lines, can there is not proterties and separate in self progeny, therefore in breeding, have very high using value.
The process that the invention has the beneficial effects as follows seed collecting and seed selection can be rejected the palm seed of being attacked by damage by disease and insect and there is no supermature palm seed, thus the emergence rate can ensure the plantation of palm seed time; The cold-resistant processing of palm seed can improve the stability of palm seed cell film and improve the activity of antioxidase in cell, thereby the cold resistance of the palm seedling growing after palm seed germination is improved; During low temperature cycling was can make palm seedling obtain gradually better Cold Acclimation, and good cold tolerance palm seedling can be screened, basis is provided to the cultivation of cold-resistant palm seedling, cold-resistant palm seedling has the trunk more sturdy than common palm and leaf, its cold resistance is increased, resist outside adverse circumstances ability and strengthen, and can also improve drought-resistant ability in the cold resistant while of increase.
Embodiment
Embodiment 1
1) collect seed: while selecting wild cold-resistant palm to start to have seed shedding, start to collect seed, reject near the described cold-resistant palm fruit that has granule worm excrement the outside and carpopodium of cold-resistant palm fruit, pluck intact described cold-resistant palm fruit, for subsequent use;
2) seed selection: will be described after collecting seed cold-resistant palm fruit immersion 36h, by the cold-resistant palm fruit emerging drag for need not, select good described cold-resistant palm fruit the threshing of being dried in the shade of sinking to obtain cold-resistant palm seed;
3) the cold-resistant processing of seed: be first that 0.2% potassium permanganate is to described cold-resistant palm seed disinfection 15min with mass percent concentration, sterilization is rear with cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, the magnesium chloride solution that described cold-resistant palm seed is placed in to 30mg/L soaks 12h, and described cold-resistant palm seed sand is embedded in to 36h at 35～45 DEG C, finally use cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, for subsequent use;
4) vernalization: before sowing,, with the water seed soaking 2h of 40～50 DEG C, the water seed soaking 20h of 20 DEG C, pulls described cold-resistant palm seed out, the moisture that dries in the shade, afterwards with 3% glycerite immersion 10min, pulls the described cold-resistant palm seed moisture that dries in the shade out, for subsequent use;
5) sowing: first assorted soil excrement, yellow ground and cultivation Soil tillage were evenly made to Nutrition Soil before sowing after, nitrolim is evenly spread fertilizer over the fields on described Nutrition Soil and carries out sterilization treatment to described Nutrition Soil, described Nutrition Soil after sterilizing fertilising is installed in plant planting bag, afterwards 2～3, described cold-resistant palm seed after treatment described vernalization is seeded in 1 described plant planting bag, on described cold-resistant palm seed, thickness of earth covering is 18cm;
6) seedling management: after emerging, watered once every 1 day, sprayed 5%666 liquids every 2 weeks, after emerging month, the described plant planting bag of not emerging is removed, by cold-resistant palm seedling splitting plantation in the described planting bag of 2 of emerging;
7) domestication by low temperature: in the time that cold-resistant palm seedling grows tall 30cm, described cold-resistant palm seedling is planted and cultivated in canopy soil, spacing in the rows is 0.8m, and described cultivation temperature of shed is adjusted into 4～15 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-5～10 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-15～0 DEG C, cultivate six months;
8) cold-resistant palm haploid breeding: the described cold-resistant palm seedling that described domestication by low temperature is finished to rear survival was transplanted in natural environment in spring, grow up to after ripe cold-resistant palm until described cold-resistant palm seedling, after staminiferous plant in described cold-resistant palm blooms, gathering its pollen and carry out vitro anther culture and obtain monoploid seedling, is that 0.4% colchicine obtains palm seedling and is purebred described cold-resistant palm to spraying mass percent concentration on described monoploid seedling; Female plant in described cold-resistant palm carries out Plant Tissue Breeding and obtains a large amount of described cold-resistant palms.
Embodiment 2
1) collect seed: while selecting wild cold-resistant palm to start to have seed shedding, start to collect seed, reject near the described cold-resistant palm fruit that has granule worm excrement the outside and carpopodium of cold-resistant palm fruit, pluck intact described cold-resistant palm fruit, for subsequent use;
2) seed selection: will be described after collecting seed cold-resistant palm fruit immersion 48h, by the cold-resistant palm fruit emerging drag for need not, select good described cold-resistant palm fruit the threshing of being dried in the shade of sinking to obtain cold-resistant palm seed;
3) the cold-resistant processing of seed: be first that 0.5% potassium permanganate is to described cold-resistant palm seed disinfection 20min with mass percent concentration, sterilization is rear with cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, the magnesium chloride solution that described cold-resistant palm seed is placed in to 40mg/L soaks 18h, and described cold-resistant palm seed sand is embedded in to 48h at 45 DEG C, finally use cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, for subsequent use;
4) vernalization: before sowing,, with the water seed soaking 3h of 50 DEG C, the water seed soaking 24h of 30 DEG C, pulls described cold-resistant palm seed out, the moisture that dries in the shade, afterwards with 5% glycerite immersion 10min, pulls the described cold-resistant palm seed moisture that dries in the shade out, for subsequent use;
5) sowing: first assorted soil excrement, yellow ground and cultivation Soil tillage were evenly made to Nutrition Soil before sowing after, nitrolim is evenly spread fertilizer over the fields on described Nutrition Soil and carries out sterilization treatment to described Nutrition Soil, described Nutrition Soil after sterilizing fertilising is installed in plant planting bag, afterwards 3, described cold-resistant palm seed after treatment described vernalization is seeded in 1 described plant planting bag, on described cold-resistant palm seed, thickness of earth covering is 22cm;
6) seedling management: after emerging, watered once every 2 days, sprayed 7%666 liquids every 3 weeks, after emerging month, the described plant planting bag of not emerging is removed, by cold-resistant palm seedling splitting plantation in the described planting bag of 2～3 of emerging;
7) domestication by low temperature: in the time that cold-resistant palm seedling grows tall 30～50cm, described cold-resistant palm seedling is planted and cultivated in canopy soil, spacing in the rows is 0.8～1.0m, and described cultivation temperature of shed is adjusted into 4～15 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-5～10 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-15～0 DEG C, cultivate six months;
8) cold-resistant palm haploid breeding: the described cold-resistant palm seedling that described domestication by low temperature is finished to rear survival was transplanted in natural environment in spring, grow up to after ripe cold-resistant palm until described cold-resistant palm seedling, after staminiferous plant in described cold-resistant palm blooms, gathering its pollen and carry out vitro anther culture and obtain monoploid seedling, is that 0.6% colchicine obtains palm seedling and is purebred described cold-resistant palm to spraying mass percent concentration on described monoploid seedling; Female plant in described cold-resistant palm carries out Plant Tissue Breeding and obtains a large amount of described cold-resistant palms.
Embodiment 3
1) collect seed: while selecting wild cold-resistant palm to start to have seed shedding, start to collect seed, reject near the described cold-resistant palm fruit that has granule worm excrement the outside and carpopodium of cold-resistant palm fruit, pluck intact described cold-resistant palm fruit, for subsequent use;
2) seed selection: will be described after collecting seed cold-resistant palm fruit immersion 48h, by the cold-resistant palm fruit emerging drag for need not, select good described cold-resistant palm fruit the threshing of being dried in the shade of sinking to obtain cold-resistant palm seed;
3) the cold-resistant processing of seed: be first that 0.5% potassium permanganate is to described cold-resistant palm seed disinfection 20min with mass percent concentration, sterilization is rear with cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, the magnesium chloride solution that described cold-resistant palm seed is placed in to 35mg/L soaks 18h, afterwards described cold-resistant palm seed sand is buried to 48h temperature and remain on 40 DEG C, finally use cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, for subsequent use;
4) vernalization: before sowing,, with the water seed soaking 2.5h of 45 DEG C, the water seed soaking 24h of 30 DEG C, pulls described cold-resistant palm seed out, the moisture that dries in the shade, afterwards with 5% glycerite immersion 10min, pulls the described cold-resistant palm seed moisture that dries in the shade out, for subsequent use;
5) sowing: first assorted soil excrement, yellow ground and cultivation Soil tillage were evenly made to Nutrition Soil before sowing after, nitrolim is evenly spread fertilizer over the fields on described Nutrition Soil and carries out sterilization treatment to described Nutrition Soil, described Nutrition Soil after sterilizing fertilising is installed in plant planting bag, afterwards 2, described cold-resistant palm seed after treatment described vernalization is seeded in 1 described plant planting bag, on described cold-resistant palm seed, thickness of earth covering is 20cm;
6) seedling management: after emerging, watered once every 1 day, sprayed 7%666 liquids every 3 weeks, after emerging month, the described plant planting bag of not emerging is removed, by cold-resistant palm seedling splitting plantation in the described planting bag of 2 of emerging;
7) domestication by low temperature: in the time that cold-resistant palm seedling grows tall 30～50cm, described cold-resistant palm seedling is planted and cultivated in canopy soil, spacing in the rows is 0.8～1.0m, and described cultivation temperature of shed is adjusted into 4～15 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-5～10 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-15～0 DEG C, cultivate six months;
8) cold-resistant palm haploid breeding: the described cold-resistant palm seedling that described domestication by low temperature is finished to rear survival was transplanted in natural environment in spring, grow up to after ripe cold-resistant palm until described cold-resistant palm seedling, after staminiferous plant in described cold-resistant palm blooms, gathering its pollen and carry out vitro anther culture and obtain monoploid seedling, is that 0.5% colchicine obtains palm seedling and is purebred described cold-resistant palm to spraying mass percent concentration on described monoploid seedling; Female plant in described cold-resistant palm carries out Plant Tissue Breeding and obtains a large amount of described cold-resistant palms.
The various embodiments described above result is as follows:
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in specification and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment describing.
Claims (8)
1. a breeding method for cold-resistant palm, is characterized in that, comprises the following steps:
1) collect seed: while selecting wild cold-resistant palm to start to have seed shedding, start to collect seed, reject near the described cold-resistant palm fruit that has granule worm excrement the outside and carpopodium of cold-resistant palm fruit, pluck intact described cold-resistant palm fruit, for subsequent use;
2) seed selection: will be described after collecting seed cold-resistant palm fruit immersion 36～48h, by the cold-resistant palm fruit emerging drag for need not, select good described cold-resistant palm fruit the threshing of being dried in the shade of sinking to obtain cold-resistant palm seed;
3) the cold-resistant processing of seed: be first that 0.2～0.5% potassium permanganate is to described cold-resistant palm seed disinfection 15～20min with mass percent concentration, sterilization is rear with cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, the magnesium chloride solution that described cold-resistant palm seed is placed in to 30～40mg/L soaks 12～18h, afterwards described cold-resistant palm seed sand is buried to 36～48h temperature and remain on 35～45 DEG C, finally use cold-resistant palm seed described in distilled water flushing, dry in the shade after described cold-resistant palm the surface of the seed moisture, for subsequent use;
4) vernalization: before sowing,, with the water seed soaking 2～3h of 40～50 DEG C, the water seed soaking 20～24h of 20～30 DEG C, pulls described cold-resistant palm seed out, moisture dries in the shade, soak 10min with 3～5% glycerites afterwards, pull the described cold-resistant palm seed moisture that dries in the shade out, for subsequent use;
5) sowing: first assorted soil excrement, yellow ground and cultivation Soil tillage were evenly made to Nutrition Soil before sowing after, nitrolim is evenly spread fertilizer over the fields on described Nutrition Soil and carries out sterilization treatment to described Nutrition Soil, described Nutrition Soil after sterilizing fertilising is installed in plant planting bag, afterwards 2～3, described cold-resistant palm seed after treatment described vernalization is seeded in 1 described plant planting bag, on described cold-resistant palm seed, thickness of earth covering is 18～22cm;
6) seedling management: after emerging, watered once every 1～2 day, sprayed 5～7%666 liquids every 2～3 weeks, after emerging one month, the described plant planting bag of not emerging is removed, by cold-resistant palm seedling splitting plantation in the described planting bag of 2～3 of emerging;
7) domestication by low temperature: in the time that cold-resistant palm seedling grows tall 30～50cm, described cold-resistant palm seedling is planted and cultivated in canopy soil, spacing in the rows is 0.8～1.0m, and described cultivation temperature of shed is adjusted into 4～15 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-5～10 DEG C, cultivate and afterwards the described cold-resistant palm seedling of death is removed for six months, and described cultivation temperature of shed is adjusted into-15～0 DEG C, cultivate six months;
8) cold-resistant palm haploid breeding: the described cold-resistant palm seedling that described domestication by low temperature is finished to rear survival was transplanted in natural environment in spring, grow up to after ripe cold-resistant palm until described cold-resistant palm seedling, after staminiferous plant in described cold-resistant palm blooms, gathering its pollen and carry out vitro anther culture and obtain monoploid seedling, is that 0.4～0.6% colchicine obtains palm seedling and is purebred described cold-resistant palm to spraying mass percent concentration on described monoploid seedling; Female plant in described cold-resistant palm carries out Plant Tissue Breeding and obtains a large amount of described cold-resistant palms.
2. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 2) described cold-resistant palm fruit immersion 48h.
3. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 3) described cold-resistant palm seed is placed in the magnesium chloride solution of 35mg/L.
4. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 4) sow the water seed soaking 2h with 45 DEG C before described cold-resistant palm seed.
5. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 5) on cold-resistant palm seed, thickness of earth covering is 20cm described in when sowing.
6. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 5) soil assorted excrement, described yellow ground and described cultivation soil volume ratio 1～2:5～7:12～13 described in Nutrition Soil described in when sowing.
7. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 5) plant planting bag specification is 15 × 20cm described in when sowing.
8. the breeding method of cold-resistant palm as claimed in claim 1, is characterized in that, described step 8) described colchicine mass percent concentration is 0.5%.
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| CN104221684A (en) * | 2014-09-19 | 2014-12-24 | 郎溪庆林生态特色农业观光园有限公司 | Palm seeding and breeding method |
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| CN101669424B (en) * | 2008-09-13 | 2011-05-18 | 棕榈园林股份有限公司 | Method for protecting palms to pass winter in low temperature environment |
| MY185898A (en) * | 2010-03-23 | 2021-06-14 | Crop Microclimate Man Inc | Methods for increasing tolerance to abiotic stress in plants |
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