CN103392634A - Molecular and genetic engineering technology for cultivating colorful fluorescent pearls - Google Patents
Molecular and genetic engineering technology for cultivating colorful fluorescent pearls Download PDFInfo
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- CN103392634A CN103392634A CN2013102536451A CN201310253645A CN103392634A CN 103392634 A CN103392634 A CN 103392634A CN 2013102536451 A CN2013102536451 A CN 2013102536451A CN 201310253645 A CN201310253645 A CN 201310253645A CN 103392634 A CN103392634 A CN 103392634A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a molecular and genetic engineering technology for cultivating colorful fluorescent pearls, and relates to a technology for cultivating bright-colored pearls and fluorescent pearls. The preparing technology includes the steps that a chemical self-assembled technology is used, natural dye molecules (algas rich in natural dye molecules are selected to be used as feed) are led in during the process of pearl forming, self-assembling is achieved on organic matrixes of pearl layers, and therefore the purpose of dying the pearls which are finally formed is achieved. Natural dye is used for conducting dyeing on the pearl layers, so that various colorful pearls can be achieved in a controllable mode. Through the modern genetic technology, fluorescent protein which is used widely in the biological chemistry scientific researches is applied to pearl oyster mantle tissue, and therefore the pearls with various fluorescent effects can be produced.
Description
Technical field
What the present invention relates to is a kind of technique of cultivating multiple bright-colored pearl and fluorescence pearl, is specifically related to a kind of molecule and technique for gene engineering and cultivates colored and fluorescence pearl.
Background technology
The color of pearl is relatively dull, calcium carbonate (aragonite structure) crystallization of the basic structure of natural peral on albumen substrate, and the Essential colour of aragonite is exactly white, so the color of pearl can not taken off the keynote with Bai Weizhu.Even if the inserted sheet of development in recent years is cultivated the colored pearl new technology, resulting pearl color is also partially light, and seriously relies on the original color of film-making nacre, is difficult to produce new color.In addition, also have the cultivation time long, running cost is high, the shortcomings such as formality trouble.It is all that some are with active mineral that modern science is verified most so-called legendary luminous pearls, and often contact is to healthy unfavorable.
Summary of the invention
For the deficiency that exists on prior art, the present invention seeks to be to provide a kind of molecule and technique for gene engineering to cultivate colored and fluorescence pearl,
To achieve these goals, the present invention realizes by the following technical solutions: molecule and technique for gene engineering are cultivated colored and fluorescence pearl, its preparation technology is: utilize the Chemical self-assembly technology, in the process that pearl forms, introduce natural dye molecule (can select to be rich in the algae of natural dye molecule as feed), on the organic substrate of nacre, realize self assembly, thereby the pearl of final formation is reached to the purpose of dyeing.
According to above-mentioned molecule and technique for gene engineering, cultivate colored and fluorescence pearl, wherein, in described colour and fluorescence pearl, also be added with fluorescence protein gene, by genetic modification, this fluorescin can go down in heredity, and the offspring continues to keep producing the ability of fluorescence pearl.
Dyeing controllably realizes diversified colored pearl to nacre thereby the present invention utilizes natural dye.And, by modern gene technology, in pearl shell mantle tissue, just can produce the pearl of various fluorescent effects to the fluorescent protein expression that has extensively utilized in the biochemistry scientific research.
The accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, describe the present invention in detail;
Fig. 1 is the molecular structure of the luteole in the present invention.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with embodiment, further set forth the present invention.
With reference to Fig. 1, this embodiment is by the following technical solutions: molecule and technique for gene engineering are cultivated colored and fluorescence pearl, its preparation technology is: utilize the Chemical self-assembly technology, in the process that pearl forms, introduce natural dye molecule (can select to be rich in the algae of natural dye molecule as feed), on the organic substrate of nacre, realize self assembly, thereby the pearl of final formation is reached to the purpose of dyeing.
It should be noted that in described colour and fluorescence pearl and also be added with fluorescence protein gene, by genetic modification, this fluorescin can go down in heredity, and the offspring continues to keep producing the ability of fluorescence pearl.
The concrete technology flow process of this embodiment is: at first pearl food diatom commonly used is improved, change over to and can synthesize carotin, lycopene, luteole, the gene of the natural organic molecule such as fluorescin and the gene of other modification groups enter in the genome of diatom, these natural dye groups with can with the polynary cyclic group of calcium ion chelating and the modification of close carboxylic group.And then with the diatom that these genetic modifications are crossed, feed the pearl freshwater mussel in the suitable time of pearl freshwater mussel normal development, when allowing the pearl freshwater mussel can absorb the diatom nutriment, digested the breaking of diatom, discharge the natural organic dye molecule of modified, in pearl freshwater mussel body, form high local concentrations natural dye molecule environment, these natural dye molecules will contain amino acid whose pearl surface self assembly, suction-operated by relevant group is being paid on the pearl surface that is just gradually growing up, or is formed on simultaneously pearl surface with the organic calcium inorganic calcium.This process constantly repeats, and will generate enough sizes, the uniform pearl of natural dye distribution of color.
Pearl in this embodiment is a kind of composite of natural organic/inorganic lamellar multilevel structure.Wherein volume accounts for 5% organic substrate (protein, polysaccharide, lipid etc.) composition template, and then by molecular recognition mechanism, inorganic molecule calcium carbonate deposits and forms sheet aragonite structure on template.These sheet aragonite structures present polygonal shape, and are cross layered, are piled into orderly 3-D solid structure.Utilize the Chemical self-assembly technology, in the process that pearl forms, introduce natural dye molecule (can select to be rich in the algae of natural dye molecule as feed), on the organic substrate of nacre, realize self assembly, thereby the pearl of final formation is reached to the purpose of dyeing.The selection of natural dye molecule can be a lot, such as the luteole of structure shown in Figure 1 (carotenoid a kind of) can show a kind of bright yellow.Various types of carotin extensively is present in the middle of various wholefoods, to human body and environmentally friendly, and also low price, easily obtain, be the good selection of dyeing pearl agent.By deleting, select the natural dye library of molecules, be not difficult to obtain the dyeing pearl agent molecule of other colors.
The present embodiment, can send the fluorescence with particular color with the biology of fluorescin under illumination.And through years of researches, scientists has been developed the fluorescin with red, yellow, blue etc. multiple color.Related gene and expression technology have become a kind of regular job of maturation.So we can utilize correlation technique, fluorescent protein expression in the pearl shell body, thereby realize having the pearl of multiple color fluorescence.By genetic modification, this fluorescin can go down in heredity, and the offspring continues to keep producing the ability of fluorescence pearl, is the technology of putting things right once and for all.
Dyeing controllably realizes diversified colored pearl to nacre thereby the present invention utilizes natural dye.And, by modern gene technology, in pearl shell mantle tissue, just can produce the pearl of various fluorescent effects to the fluorescent protein expression that has extensively utilized in the biochemistry scientific research.
Above demonstration and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (3)
1. molecule and technique for gene engineering are cultivated colored and fluorescence pearl, it is characterized in that, its preparation technology is: utilize the Chemical self-assembly technology, in the process that pearl forms, introduce the natural dye molecule, on the organic substrate of nacre, realize self assembly, thereby the pearl of final formation is reached to the purpose of dyeing.
2. molecule according to claim 1 and technique for gene engineering are cultivated colored and fluorescence pearl; it is characterized in that; colored and prepared by fluorescence pearl concrete technology flow process is: at first pearl food diatom commonly used is improved; change over to and can synthesize carotin; lycopene; luteole; the gene of the natural organic molecule such as fluorescin and the gene of other modification groups enter in the genome of diatom, these natural dye groups with can with the polynary cyclic group of calcium ion chelating and the modification of close carboxylic group; And then with the diatom that these genetic modifications are crossed, feed the pearl freshwater mussel in the suitable time of pearl freshwater mussel normal development; when allowing the pearl freshwater mussel can absorb the diatom nutriment; digested the breaking of diatom, discharge the natural organic dye molecule of modified; in pearl freshwater mussel body, form high local concentrations natural dye molecule environment; these natural dye molecules will contain amino acid whose pearl surface self assembly; suction-operated by relevant group is being paid on the pearl surface that is just gradually growing up, or is formed on simultaneously pearl surface with the organic calcium inorganic calcium; This process constantly repeats, and will generate enough sizes, the uniform pearl of natural dye distribution of color.
3. molecule according to claim 1 and technique for gene engineering are cultivated colored and fluorescence pearl, it is characterized in that, in described colour and fluorescence pearl, also be added with fluorescence protein gene, by genetic modification, this fluorescin can go down in heredity, and the offspring continues to keep producing the ability of fluorescence pearl.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548744A (en) * | 2013-11-26 | 2014-02-05 | 浙江长生鸟珍珠生物科技有限公司 | Method for cultivating color fresh water pearls with nucleuses |
| CN104737954A (en) * | 2015-04-09 | 2015-07-01 | 广东海洋大学 | Super-green seawater pearl cultivation method |
| CN104756917A (en) * | 2015-04-10 | 2015-07-08 | 绍兴文理学院 | Cultivating method of hyriopsis cumingii pearls |
| CN106577416A (en) * | 2016-12-08 | 2017-04-26 | 金华职业技术学院 | Cultivation method of fluorescent protein-based luminous pearl |
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| JPH09327252A (en) * | 1995-12-14 | 1997-12-22 | De Iru I | Pearl |
| EP1163844A1 (en) * | 1999-02-25 | 2001-12-19 | Miki, Keizaburo | Transgenic mollusc and method for constructing the same |
| CN1647625A (en) * | 2004-03-15 | 2005-08-03 | 邰港科技股份有限公司 | Method for producing new fluorescent fish and new fluorescent fish produced by said method |
| JP2007000047A (en) * | 2005-06-22 | 2007-01-11 | Univ Of Miyazaki | Method for production of cultured pearl |
| CN101243777A (en) * | 2008-03-13 | 2008-08-20 | 苏州市珍妮日用化工有限公司 | Noctilucence pearl cultivating method |
| CN102258239A (en) * | 2011-04-29 | 2011-11-30 | 广东海洋大学 | Method for processing colorized pearls |
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2013
- 2013-06-25 CN CN2013102536451A patent/CN103392634A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09327252A (en) * | 1995-12-14 | 1997-12-22 | De Iru I | Pearl |
| EP1163844A1 (en) * | 1999-02-25 | 2001-12-19 | Miki, Keizaburo | Transgenic mollusc and method for constructing the same |
| CN1647625A (en) * | 2004-03-15 | 2005-08-03 | 邰港科技股份有限公司 | Method for producing new fluorescent fish and new fluorescent fish produced by said method |
| JP2007000047A (en) * | 2005-06-22 | 2007-01-11 | Univ Of Miyazaki | Method for production of cultured pearl |
| CN101243777A (en) * | 2008-03-13 | 2008-08-20 | 苏州市珍妮日用化工有限公司 | Noctilucence pearl cultivating method |
| CN102258239A (en) * | 2011-04-29 | 2011-11-30 | 广东海洋大学 | Method for processing colorized pearls |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548744A (en) * | 2013-11-26 | 2014-02-05 | 浙江长生鸟珍珠生物科技有限公司 | Method for cultivating color fresh water pearls with nucleuses |
| CN104737954A (en) * | 2015-04-09 | 2015-07-01 | 广东海洋大学 | Super-green seawater pearl cultivation method |
| CN104737954B (en) * | 2015-04-09 | 2017-09-29 | 广东海洋大学 | A kind of breeding method of emerald green sea water pearls |
| CN104756917A (en) * | 2015-04-10 | 2015-07-08 | 绍兴文理学院 | Cultivating method of hyriopsis cumingii pearls |
| CN104756917B (en) * | 2015-04-10 | 2017-07-21 | 绍兴文理学院 | A kind of breeding method of Margarita |
| CN106577416A (en) * | 2016-12-08 | 2017-04-26 | 金华职业技术学院 | Cultivation method of fluorescent protein-based luminous pearl |
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Application publication date: 20131120 |