CN103384477B - For having the composition of the functional food recovering blood constitute and function - Google Patents

For having the composition of the functional food recovering blood constitute and function Download PDF

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Publication number
CN103384477B
CN103384477B CN201280005658.0A CN201280005658A CN103384477B CN 103384477 B CN103384477 B CN 103384477B CN 201280005658 A CN201280005658 A CN 201280005658A CN 103384477 B CN103384477 B CN 103384477B
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food composition
healthy functions
composition
functions food
phloroglucin
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CN103384477A (en
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李涬雨
慎贤哲
金成镐
玄宗勋
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BOTAMEDI Inc
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
    • A23L29/281—Proteins, e.g. gelatin or collagen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to for be effective to recover due to anticancer therapy, operation, aging or stress and the composition of functional food of the blood constitute and function of decline.Composition for functional food comprises the Cobastab of the collagen of (with dry weight) 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and 0.01 ~ 0.1wt%.

Description

For having the composition of the functional food recovering blood constitute and function
Technical field
The present invention relates to for have efficient recovery due to chemotherapy, operation, chronic disease, over training stress or healthy functions food that is aging and the serology constitute and function be lowered.
The application's book requires the rights and interests of the Korean Patent Application No. 10-2011-0003566 that on January 13rd, 2011 submits to, and its full content is incorporated to the application as a reference.
Background technology
In the past in many decades, develop much outstanding anticancer and anti-cancer therapies by chemical synthesis or by separating natural material.These effort significantly improve the cure rate of cancer in this in period.But concentrate on the damage of removing localized cancer, conventional cancer therapy causes the problem reducing patients ' life quality.In recent years, the deterioration needing the quality of life improving chemotherapy or radiotherapy induction is proposed.
Since nineteen fifty-seven synthetic chemistry therapeutic agent 5 FU 5 fluorouracil (5-FU) first, it for clinical practice so far.5-FU is transformed into active nucleotide in cancer cell, and Main Function is the effect stoping thymidylate synthase, thus DNA replication dna in inhibition cancer cell.This activating agent, clinically for wide spectrum cancer, comprises breast cancer, colorectal cancer, the carcinoma of the rectum, cancer of the stomach, prostate cancer, the cancer of the esophagus, liver cancer, head and neck cancer and carcinoma of urinary bladder, and also local is used for cutaneum carcinoma.But this activating agent causes significant side effect, comprises hematopoietic toxicity.In addition, 5-FU has negative effect to digestive system, causes high-frequency gastritis, esophagitis, rectitis, diarrhoea etc.And, find that the patient using 5-FU suffers melanocyte to increase disease.Except hematopoietic toxicity (such as Neuroleptic Leukocytopenia) and gastrointestinal toxicity (in such as intestinal mucosa, sucrose lacks and diarrhoea), report the side effect of immunotoxicity (such as spleen weight reduction, lymphocyte minimizing etc.) as 5-FU.
Other representative chemotherapeutic agents also been observed significant side effect, and described chemotherapeutant is adriamycin, bleomycin, mitomycin and cis-platinum such as.These activating agents of chronic administration cause reversible or irreversible DNA damage, such as, suppress DNA replication dna and cell division, cell membrane damage etc.
Report the cancer patient that the whole world is tens million of at present, and there is significant side effect in nearly all anti-cancer therapies being applied to them.Chemotherapy is usually designed to the cancer cell killing division fast, but also destroys the normal cell with the short cell cycle, such as haemocyte.In chemotherapy side effect, most is representative and have plenty of the blood function obstacle that hematoclasis causes altogether.Such as, patient is easy to reduced by decrease of platelet, Neuroleptic Leukocytopenia and immunity usually, and this causes quality of life to decline.
In radiotherapy, the radiation of high dose also causes leucocyte and platelet levels and immunity to reduce, and causes the Antioxidative Defense System dysfunction of blood.The operative treatment of cancer also has negative effect to blood health.In operation, medicine (such as whole body or local anesthetic, antihemorrhagic, anticoagulation and anodyne) and oxidative stress (the temporary transient imbalance of such as metabolism and temporary transient hemorrhagic ischemic) active oxygen classification (OH, NO must be produced 2deng), bring out side effect subsequently.
Therefore, recovering blood constitute and function (such as immunity and anti-oxidative defense) is very important for effectively getting well in the side effect from anti-cancer therapies and operation.In addition, the patient of chronic disease, the elderly, weakling and continuously and the people of over training shows the serology problem of unstable serology composition and low immunity or anti-oxidative defense function is suffered from.Recover blood constitute and function for a restorative prerequisite.
A lot of recovery meals are only suitable for providing enough required nutrients or polyphenoils, or comprise raw material, and its chemical composition is unknown (Korean patent No. 0722698,0591539 and 0355012).Korean patent No. 0519456 relates to the composition for suppressing anticancer therapy side effect, and it is designed to improve blood cell levels and immunity, and described haemocyte is leucocyte, red blood cell and blood platelet such as.But, composition to the anti-oxidative defense (important indicator of Health restoration) recovering to mention in this patent without any effect.In addition, because composition contains multiple grass, its composition is difficult to clear and definite standardization and in food.
For the present invention, the present inventor to the nutrition of Multiple components, herbal medicine with and biochemical property emphasis and thoroughly research obtain such discovery, generate, from blood, remove inflammatory factor and promote that blood antioxidant function can overcome the problem run in prior art as the concrete composition of the Multiple components of food, haemocyte, and be considered to as food composition for there being efficient recovery serology constitute and function to be very effective.
Open
Technical problem
In order to overcome the problem run in prior art, the object of this invention is to provide healthy functions food composition, it comprises the Cobastab of the collagen of 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin (phlorotannin) of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and 0.01 ~ 0.1wt%.
Another object of the present invention is to provide healthy functions food, and wherein based on the healthy functions food composition of 100 weight portions, the use amount of excipient is from 1 to 1,000 weight portion.
Technical scheme
According to an one aspect, the invention provides healthy functions food composition, it comprises the Cobastab of the collagen of 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and 0.01 ~ 0.1wt%.
Before healthy functions food composition of the present invention can be applicable to just to experience anticancer therapy, experience anticancer therapy or after experience anticancer therapy, suffer chronic disease, the physically-draining that maybe stress cause due to over training or the individuality of senile debility.
In addition, healthy functions food composition of the present invention can be mixed with the form being suitable for absorbing, such as tablet, capsule, pill, granule, liquid, beverage, liquid dietary, soup, bread product, rod and cooky.
According to its another aspect, the invention provides the healthy functions food comprising excipient, based on the healthy functions food composition of 100 weight portions, the amount of excipient is from 1 to 1,000 weight portion.
Beneficial effect
When taking in, healthy functions food composition of the present invention may be used for improving the blood dyscrasia constitute and function obstacle that many factors (comprise medicine, stress wait) causes, thus in serology composition and anti-oxidative defense ability recovery blood to normal condition.
Best mode
The present invention is described below in detail.
According to an one aspect, the invention provides healthy functions food composition, it comprises the Cobastab of the collagen of 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and 0.01 ~ 0.1wt%.
Collagen is the scleroprotein found in the bone of animal and cartilage and fish scale.It is the fibrillation form extended, and water insoluble, but is irreversibly hydrolyzed into gelatin when boiling.The feature of this protein is the proline of high-load, hydroxyproline and glycine.In the present invention, collagen is used as the stock that haemocyte generates, and as protein source, promotes the generation of albumin and immunoglobulin (Ig) in blood.If the use amount of collagen is less than 30wt%, its effect is not enough to play.On the other hand, unfavorable economically more than the collagen content of 90wt%.
Collagen used in the present invention derived from fish or meat, but can not limit it.
Flax is annual herb, and it is Dicotyledoneae Mang ox seedling order flax family (Linaceae) member, and has production from Europe to Argentina.The seed of flax, also referred to as linseed, is rich in phytoestrogen lignan and phytoprotein, and therefore they contribute to producing albumin and globulin, thus improve immunity.Linseed content is lower than the effect that can not obtain linseed during 3wt%.On the other hand, the composition containing the linseed being greater than 60wt% amount is difficult to be mixed with required form.
In the present invention, linseed can use as a powder or in liquid form, but does not limit it.
Phloroglucin tannin, the class tannin existed in brown alga is potential antioxidant, has the middle character between water and lipid solubility.Ability phloroglucin tannin being recovered to vascular function or removing inflammatory factor has a lot of report.
Phloroglucin tannin available in the present invention can be selected from but be not limited to goose palm dish phenol (eckol), two goose palm dish phenol, 6,6 '-bis-goose palm dish phenol, 8,8 '-bis-goose palm dish phenol, 2-O-(2,4,6-trihydroxy phenyl)-6,6 '-bis-goose slaps dish phenol, phloroglucin basement rock algae furans goose palm dish phenol (phlorofucofuroeckol), rock algae furans goose palm dish phenol (fucofuroeckol), 2-phloroglucin base goose palm dish phenol, 7-phloroglucin base goose slaps dish phenol, three phloroglucin base geese slap dish phenol, phloroglucin tannin A and combination thereof.
In the present invention, the effect of phloroglucin tannin is there is no when phloroglucin tannin amount is less than 1wt%.On the other hand, economic benefits is not had more than the phloroglucin tannin content of 50wt%.
Calcium, in periodic table Central Plains, subnumber is the alkaline-earth metal of 20, is the required inorganic nutritive element controlling body basic activity (such as blood clotting, contraction of muscle etc.).It is one of nutrient that in meals, most probable lacks, and for adult, its daily intake of recommending is 700mg.
Calcium content lower than 1wt% can not play enough calcium effects.On the other hand, when it contains the calcium higher than 30wt% amount, composition is difficult to be mixed with required form.
Iron is periodic table Central Plains subnumber is the metal of 26, and is used as required inorganic nutritive element, and it is that the physiology course of O_2 transport and cellular respiration is necessary, and plays a significant role in generation red blood cell.For adult female, the daily intake of the recommendation of iron is 12 ~ 15mg.
In the present invention, there is no effect when iron content is less than 0.1wt%, and make composition be difficult to be mixed with required form higher than the iron content of 3wt%, or negative effect is existed to the color of preparation or effective life.
Vitamin E, also referred to as tocopherol, as fat-soluble antioxidant.
When its amount is less than 0.01wt%, there is no obvious vitamin E effect, and easily produce toxicity more than the content of vitamin E of 0.5wt%.
There is the Cobastab of different subtype.It plays a significant role in immune system and cellular metabolism.In the present invention, there is no obvious effect when Cobastab amount is less than 0.01wt%, and there is negative effect more than the effective life of Cobastab content to preparation of 0.1wt%.
Healthy functions food composition of the present invention, except comprising the Cobastab of the collagen of 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and 0.01 ~ 0.1wt%, can comprise the lecithin of 1 ~ 10wt%, the lignan of 0.005 ~ 3wt%, the copper of 0.01 ~ 0.2wt% further.
Lecithin is the key component of a kind of phosphatide, cell membrane, and is present in a large number in yolk, soya-bean oil, liver and brain.
Lignan is the adopted name of the natural products much with the structure formed by connecting two positive phenyl-propane residues on the beta carbon of n-pro-pyl side chain.It is phytoestrogen and is used as antioxidant.
Copper is the metallic element of periodic table Central Plains subnumber 29, and contributes to utilizing iron and producing red blood cell on physiology.For adult, the daily intake of recommendation is 1.5 ~ 3mg.
In one embodiment, in the mouse of using anticancer 5-FU, the level that composition of the present invention significantly increases the cytokine profiles related in blood platelet, leucocyte and red blood cell and immunologic facilitation is observed.
In another embodiment, be applied to team of institute 40 male sex's bicycle entrants during non-season strong training period after, have evaluated the ability that composition of the present invention recovers blood composition.Find the level significantly increasing white blood corpuscle, red blood cell, hemoglobin and hematid specific volume simultaneously.
Therefore, healthy functions food composition of the present invention can recover serology composition to normal level.
In order to assess healthy functions food composition of the present invention to the antioxidation of blood, monitor the level of biomarker of representative polyphenoils and MDA (MDA) in the glutathione of 40 male sex's bicycle entrants and catalase, blood, oxidative stress.Placebo is compared in discovery, and group glutathione and the catalase of using healthy functions food composition of the present invention add, but MDA reduces.
Therefore, healthy functions food composition of the present invention can recover the anti-oxidation function of blood to normal level.
Before healthy functions food composition of the present invention can be applicable to just to experience anticancer therapy, experience anticancer therapy or after experience anticancer therapy, suffer chronic disease, because excess body trains the individuality of physically-draining or the senile debility that maybe stress cause, but it is not limited.
In addition, healthy functions food composition of the present invention can be mixed with the form being suitable for absorbing, such as tablet, capsule, pill, granule, liquid, beverage, liquid dietary, soup, bread product, rod and cooky.
According to its another aspect, the invention provides the healthy functions food comprising excipient, based on the healthy functions food composition of 100 weight portions, figuration dosage is from 1 to 1,000 weight portion.
The example of excipient available in the present invention comprise glutinous rice, rice powder, flour, honey, avicel cellulose, silica, crystal diabetin, anhydrous citric acid, synanthrin, vitamin C, sesame oil, soy sauce, sugar, starch, gelatinized starch, microcrystalline cellulose, lactose, PVP, cataloid, calcium monohydrogen phosphate, sweet mellow wine, wheat gluten (wheat gluten), Arabic gum, pregelatinized starch, cornstarch, cellulose powder, hydroxypropyl cellulose, , primojel, Brazil wax, the alumina silicate of synthesis, stearic acid, dolomol, aluminum stearate, calcium stearate, dextrose, sorbierite and talcum powder, but it not to be limited.Based on the composition of 100 weight portions, the preferred use amount of excipient from 1 to 1,000 weight portion, but does not limit it.
Invention mode
By the following example, the present invention may be better understood, described embodiment for illustration of, but restriction the present invention can not be interpreted as.
Preparation embodiment 1: the preparation of composition
Prepare the multiple combination thing that constituent content is different.
Table 1
Table 2
Preparation embodiment 2: preparation
Healthy functions food composition of the present invention can be mixed with the form being suitable for absorbing as follows.
1. the preparation of pill and picked-up
Glutinous rice, flour, honey and water are mixed with the weight ratio of 1:1:0.5:3, boils and be cooled to room temperature, obtain the soup of thickness.Described soup is mixed with composition 1 with the weight ratio of 1:9, and mixture kneading is become pill, each heavy 2g.Chew picked-up 2 ball (4g) every day after the meal.
2. the preparation of tablet and picked-up
Composition 2, lactose, avicel cellulose, silica and dolomol are mixed with the weight ratio of 35:25:37:1:1, and with tablet press machine, mixture is pressed into tablet (600mg).Every day absorbs 6 to 10 after the meal.
3. the preparation of beverage and picked-up
Composition 4, crystal diabetin, anhydrous citric acid, synanthrin, vitamin C and water are mixed with the weight ratio of 2:8:1:8:0.2:160, boils 5 minutes, stir simultaneously, and cool.The beverage that every day produces with amount twice picked-up of 180mL.
4. the preparation of congee and picked-up
Glutinous rice and rice powder are mixed with the weight ratio of 1:1, and boils into congee.In the hot congee of 200g, add 2g composition 6,0.5g sesame oil and 0.5g soy sauce, and be mixed together.Absorb the congee of twice generation every day, as meals.
5. the preparation of bread product and picked-up
By the mixture kneading of 250g flour, 30g butter, 6.5g yeast, 5g salt, 37.5g sugar, 130g water and 20g composition 9, mainly 37 DEG C of fermentations, and get 30g in square position.After expanding in bread product second time sweat, they are coated with setting egg(s), and cure 15 minutes in 180 DEG C of baking boxs.Absorb the bread product produced for twice or three times every day, as meals or edible between dining.
6. the preparation of capsule and picked-up
Composition 2 is mixed with dolomol with the weight ratio of 99.8:0.2, and mixture is loaded in hard shell capsules (gelatin) with 450mg amount.Absorb 3 to 9 capsules every day.
7. the preparation of liquid dietary and picked-up
Coarse rice powder, brown alga powder, carrot soup, salt, composition 10 and water are mixed with the weight ratio of 5:1:10:10:0.1:2:200, boils and be cooled to room temperature.The liquid dietary produced is supplied to patient by plastic tube.
8. the preparation of cooky and picked-up
By 80g butter melt, and with 100g sugar and 1 egg (50g) by being uniformly mixed.180g flour, yeast powder (1/2 or about 2.5mL) and sodium bicarbonate (1/2 or about 2.5mL) are sieved and joins in mixture.Subsequently, 12g composition 3 sieved and joins in mixture, then it being cured 15 minutes at 180 DEG C.Cooky is absorbed, every day 2 to 3 times between dining.
9. the preparation of rod and picked-up
40g butter is melted, and with 40g sesame oil, 100g sugar and 100g gelatin by being uniformly mixed.180g flour, yeast powder (1/2 or about 2.5mL) and sodium bicarbonate (1/2 or about 2.5mL) are sieved and joins in mixture together with 30g walnut.Then, add the 12g composition 5 after screening, and fully mix.Dough/pasta is of a size of 3cm with 70% volume loading wide, in the aluminium foil frame that 9cm is long and 2cm is dark, and cures 15 minutes in 180 DEG C of baking boxs.Between dining, every day absorbs 2 to 3 rods.
Embodiment 1: the recovery of serum analysis constitute and function in mouse model
The inhibit activities of the anti-5 FU 5 fluorouracil of analysed composition (5-FU) side effect, described 5-FU is one of the most widely used anticancer.C57BL/6 mouse is divided into and often organizes 10, and scrape off thin layer at right flank abdomen.B16-BL6 melanoma cells is contained 5 × 10 with 0.1mL 5~ 1 × 10 6the injected sc of individual cell gives every mouse.Inject latter 7 days, form obvious tumour in skin.Then, give every mouse by a shot in 5-FU peritonaeum, subsequently by the compound 4 of preparation in the Orally administered preparation embodiment 1 of zonde, dosage is 70mg/kg, once a day, and 7 days.Complete after using, by mouse fasting 1 day, weigh and anaesthetize.(put into containing 1.4%K from heart extracting blood sample 2in the pipe of EDTA), and count red blood cell (RBC), white blood corpuscle (WBC) and blood platelet (PLT) with Coulter counter (Coulter Corporation, USA).In addition, the level of immune cell factor is measured.In this, from mouse, take out peritoneal macrophages, and with 2.5 × 10 6the density in μ L/ hole, individual cell/500 is inoculated in 24-orifice plate, and cultivates 3 hours.Removing non-adherent cell.Cultivate after 48 hours, with the IL-1 β secreted in ELISA kit quantitative culture medium and IFN-γ.Result is outlined in following table 3.
Table 3
ap<0.05, relative negative control; bp<0.01, relative negative control; *p<0.05, relatively normally; *p<0.01, relatively normally
As data are visible, compare normal control, reduce blood platelet (PLT) level 41% with 5-FU treatment separately.Compare and use separately 5-FU group, after healthy functions food composition of the present invention uses 7 days, platelet levels raises 41 ~ 58%.Therefore, find that healthy functions food composition of the present invention has recovered platelet levels to 83 ~ 93% of normal group.
For WBC, in the mouse of injecting separately 5-FU, observe WBC level decline 53%.Compare 5-FU group, use healthy functions food composition of the present invention after 7 days, WBC level raises 53 ~ 89%.Therefore, when with 5-FU combined administration, observe healthy functions food composition of the present invention and recovered WBC level to 72 ~ 89% of normal control values.
Injection 5-FU also can reduce RBC level 15%.Compare 5-FU group, in the group used together with healthy functions food composition of the present invention, RBC level significantly improves 12 ~ 15%.Therefore, when with 5-FU combined administration, observe healthy functions food composition of the present invention and recovered RBC level to 93 ~ 96% of normal control values.
For IL-1 β, the level observing it in the mouse of injecting separately 5-FU reduces by 48%.Compare 5-FU group, use healthy functions food composition of the present invention after 7 days, raise IL-1 β level 67 ~ 81%.Therefore, when with 5-FU combined administration, observe healthy functions food composition of the present invention and recovered IL-1 β level to 87 ~ 94% of normal control values.
Finally, inject 5-FU and reduce IFN-γ level 52%.Compare 5-FU group, in the group used together with healthy functions food composition of the present invention, IFN-γ level significantly improves 61 ~ 82%.Therefore, when with 5-FU combined administration, observe healthy functions food composition of the present invention and recover IFN-γ level to 77 ~ 86% of normal control values.
Consider in the lump, data show the blood constitute and function that composition of the present invention can have efficient recovery to damage due to anticancer 5-FU by the level of increasing serum component RBC, WBC and blood platelet and immune cell factor IL-1 β and IFN-γ.In addition, patient, the elderly and weakling after surgery and experience in chemotherapeutical patient and observe serology decline.Based on the result obtained, expect that those people (such as postoperative patient, the elderly etc.) that healthy functions food composition of the present invention dies down to health have effect above.
Embodiment 2: the recovery of analyzing blood composition and anti-oxidative defense potentiality in human body
When after accumulation, the exercise stress of high strength destroys red blood cell to produce higher speed than red blood cell, causes anaemia.And long-term high pressure training campaign reduces WBC level, causes immunity degradation.After long-term high pressure training campaign, although improve locomitivity, sportsman is easy to have with Post operation or experiences chemotherapy patients or the similar serology constitute and function of the elderly.Owing to observing the decline of ubiquitous serology constitute and function in those people of experience chemotherapy or experience operation and the elderly in direct sportsman after long-term high-intensity exercise, therefore, the ability that composition of the present invention recovers serology constitute and function can be assessed, if test implements with sportsman under the better condition controlled.In this, select 40 team of institute cyclist, and check serology constitute and function in high strength non-season training motion front and rear.In training period of encamping, they eat identical meals and carry out identical training program.Selected individuality within the previous moon, does not use any medicine and medical inspection is normal.Individuality is divided at random test group (n=20) and contrast (n=20).
All individualities are fully informed object and the method for this test in advance, and forbid eating outside predetermined meals, and forbid taking medicine.
Test trains between moving period in non-season to carry out one month.The composition 2 (every day 9 capsules, every capsules 450mg) of test group picked-up preparation embodiment 1 under the supervision of coach, 4 weeks.
Test example 1: blood sample collection and Serological testing
From fasting 8 hours or longer individuality, 10cc blood is gathered with disposable syringe.In the pipe that the blood sample that gathers from antecubital vein is loaded EDTA-process and serum separator tube.Obtain supernatant serum with 3,000rpm after centrifugal 15 minutes, and in whole blood and blood plasma, analyze other serology component.
Count blood cells, RBC, WBC and hemoglobin (Hb), and measure hematid specific volume (Hct).The antioxidase, particularly glutathione (GSH) in quantitative analysis blood, blood antioxidant defence potentiality are responsible for and catalase (CAT), and MDA (MDA), the biomarker of oxidative stress.
Test example 2: glutathione level
Measure the blood level of reduced glutathione (GSH) (indicants of blood antioxidant defence potentiality), as the difference between glutathione total in blood and oxidized form of glutathione level.
(reduced glutathione level=total glutathione level-oxidized form of glutathione level)
The glutathione blood level that following measurement is total.
Blood is mixed with 6% acetic acid and 10% sulfosalicylic acid, places several minutes, and with 5,000rpm centrifugal 5 minutes.Supernatant is again centrifugal, and by the supernatant dilution produced, with NADPH (nicotinamide-adenine dinucleotide phosphate, reduced form) and the DTNB (5 of predetermined amount, 5 '-two sulfo--two-(2-nitrobenzoic acid)) mixing, and 35 DEG C of incubations 5 minutes.Add the absorbance 2 minutes reading reactant mixture after the GR (glutathione reductase, Sigma G3664) of 50 μ L immediately at 412nm place.Total glutathione level is by using the measurement of GSH calibration curve to measure.
The blood level of following measurement oxidized form of glutathione (GSSG).
Blood sample is mixed with 6% acetic acid and 10% sulfosalicylic acid, places several minutes, and with 5,000rpm centrifugal 5 minutes.Supernatant is again centrifugal, and the supernatant produced and 2-VP (2-vinylpyridine, Sigma132292) are mixed by vigorous stirring, and incubation at room temperature 1 hour.By mixture diluted in buffer solution, and with NADPH and DTNB of predetermined amount 35 DEG C of incubations 5 minutes.Add the absorbance 3 minutes reading reactant mixture after the GR (glutathione reductase, Sigma G3664) of 50 μ L immediately at 412nm place.Oxidized form of glutathione level is by using the measurement of GSSG calibration curve to measure.
Test example 3: hydrogen peroxide enzyme level
Use catalase assay kit (A-22180, Molecular Probes) quantitative analysis catalase, catalase is the mark of blood antioxidant defence potentiality.When adding H in serum 2o 2during with HRP (horseradish peroxidase), Catalases catalyze is by H 2o 2be reduced into water, and HRP and H 2o 2reaction, forms resorufin, a kind of fluorescent material.Due to competition, highly active catalase reduces the signal of resorufin.The absorbance of resorufin is read at 570nm place.Hydrogen peroxide enzyme level is measured by using the measurement of (catalase) calibration curve.1U is defined as and can reduces 1 μm of ol H 2o 2enzyme amount.
Test example 4:MDA level
In order to quantitative MDA (MDA), the biomarker of oxidative stress in blood, mixes the serum of centrifugal acquisition with 10%TCA (trichloroacetic acid), boils 30 minutes in a water bath, cooling, and with 3000rpm centrifugal 10 minutes.Supernatant is mixed with 0.67%TBA (thiobarbituric acid), boils 30 minutes in a water bath, and with 3000rpm centrifugal 10 minutes.Use UV-spectrophotometer, read absorbance at 535nm place.Calibration curve is drawn with MDA-bis.
Table 4
to the restitution of serology composition
Group Before picked-up After absorbing 4 weeks
RBC(10 6/mm 3) Test group 4.72±0.28 4.88±0.22 a
RBC(10 6/mm 3) Placebo 4.73±0.31 4.63±0.26
WBC(10 3/mm 3) Test group 7.24±1.44 7.02±1.16 a
WBC(10 3/mm 3) Placebo 7.19±1.80 5.73±1.25 *
Hb(g/dL) Test group 14.41±0.83 15.06±0.73 **a
Hb(g/dL) Placebo 14.36±0.68 14.47±0.72
Hct(%) Test group 42.42±2.50 44.43±2.10 **
Hct(%) Placebo 42.27±2.13 42.30±1.86
ap<0.05, Versus Placebo; *p<0.05, relatively before picked-up; *p<0.01, relatively before picked-up
Non-season training motion is after 4 weeks, and in control group, RBC level reduces slightly, but significantly raises in the test group of picked-up composition of the present invention.For WBC, in control group, its level significantly reduces, but maintains in test group and be similar to initial level.Therefore, absorb composition energy Promote immunity power of the present invention to recover.In addition, observe the test group using composition of the present invention and significantly increase hemoglobin (Hb) level and hematid specific volume (Hct) level.Therefore, compositions table of the present invention reveals high haemocyte and generates active, as RBC level raise in further to prove.Therefore, composition of the present invention effectively can strengthen haemocyte generation and recover immunity.
Table 5
to the restitution of serum anti-oxidation function
ap<0.05, Versus Placebo; *p<0.05, relatively before picked-up; * *p<0.001, relatively before picked-up
For group (P<0.05) and period (P<0.001), there is significant difference in glutathione level.In prolonged exercise moves latter two group, blood glutathione level all increases, and test group is higher than placebo a lot.For group (P<0.05) and period (P<0.001), blood hydrogen peroxide enzyme level is also significantly different.After prolonged exercise motion, in two groups, blood hydrogen peroxide enzyme level all increases, and test group is higher than placebo a lot.
Placebo shows MDA level and significantly raises (P<0.05), but test group does not have.For group difference, placebo maintains significantly higher MDA level (P<0.05) than test group.When the oxidative stress accumulated due to prolonged exercise sports fatigue increases blood MDA level, the lower blood MDA horizontal attribution of test group is in such fact, and the picked-up present composition improves the anti-oxidation function that blood suppresses lipid oxidation.
Consider in the lump, the data obtained above show that the present composition can have efficient recovery serology anti-oxidation function, and reduce the serology oxidative stress under serious stressed condition.Therefore, expect that healthy functions food composition of the present invention helps patient, weakling, the elderly and sportsman to be recovered their health by the level increasing enzyme, described enzyme effectively can remove the multiple oxidative stress thing that anti-cancer therapies, chronic disease, aging, exercise stress etc. produce.

Claims (11)

1. healthy functions food composition, it comprises the collagen of 30 ~ 90wt%, the linseed of 3 ~ 60wt%, the phloroglucin tannin of 1 ~ 50wt%, the calcium of 1 ~ 30wt%, the iron of 0.1 ~ 3wt%, the vitamin E of 0.01 ~ 0.5wt% and the Cobastab of 0.01 ~ 0.1wt%, wherein phloroglucin tannin is selected from goose palm dish phenol, two goose palm dish phenol, 6, 6 '-bis-goose palm dish phenol, 8, 8 '-bis-goose palm dish phenol, 2-O-(2, 4, 6-trihydroxy phenyl)-6, 6 '-bis-goose palm dish phenol, phloroglucin basement rock algae furans goose palm dish phenol, rock algae furans goose palm dish phenol, 2-phloroglucin base goose palm dish phenol, 7-phloroglucin base goose palm dish phenol, three phloroglucin base goose palm dish phenol, phloroglucin tannin A and combination thereof.
2. the healthy functions food composition of claim 1, it comprises the copper of the lecithin of 1 ~ 10wt%, the lignan of 0.005 ~ 3wt% and 0.01 ~ 0.2wt% further.
3. the healthy functions food composition of claim 1, wherein collagen is derived from fish or meat.
4. the healthy functions food composition of claim 1, wherein linseed is powder or liquid form.
5. the healthy functions food composition of any one in Claims 1-4, it is for recovering serology composition or serology anti-oxidation function to normal level.
6. the healthy functions food composition of any one in Claims 1-4, its for just experience anticancer therapy, experience anticancer therapy before or experience anticancer therapy after, suffer chronic disease, because excess body trains the individuality of physically-draining or the senile debility that maybe stress cause.
7. the healthy functions food composition of any one in Claims 1-4, it is mixed with the form being selected from tablet, capsule, pill, granule, liquid, bread, rod and cooky.
8. the healthy functions food composition of any one in Claims 1-4, it is mixed with beverage.
9. the healthy functions food composition of any one in Claims 1-4, it is mixed with liquid dietary.
10. the healthy functions food composition of any one in Claims 1-4, it is mixed with congee.
11. healthy functions foods, wherein based on the healthy functions food composition of 100 weight portion claims 1, the use amount of excipient is from 1 to 1,000 weight portion.
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