CN103372076B - Treat the medicine of disease and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract that are caused by propionibacterium acnes - Google Patents
Treat the medicine of disease and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract that are caused by propionibacterium acnes Download PDFInfo
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Abstract
The invention discloses the medicine of the disease that a kind for the treatment of is caused by propionibacterium acnes and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract.Wherein, this medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose.Apply technical scheme of the present invention, Radix Sanguisorbae or Radix Sangusorbae extract preparation is adopted to treat the medicine of the disease caused by propionibacterium acnes, Radix Sanguisorbae or Radix Sangusorbae extract belong to Chinese medicine category, its have untoward reaction over the course for the treatment of low, not easily produce the advantage such as drug resistance, further, but Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes to kill effect better.
Description
Technical field
The present invention relates to field of medicaments, treat in particular to a kind of the medicine of disease and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract that are caused by propionibacterium acnes.
Background technology
Antibiotic use makes many serious bacterial infection diseases be effectively controlled, but its unreasonable use result in the increase of Resistant strain, reduces existing antibiotic use effectiveness.Chinese medicine because of its in the treatment untoward reaction low, not easily produce the advantage such as drug resistance, in widespread attention, the folk prescription with antibacterial action be made up of Chinese medicine or compound preparation and Chinese medicine and western medicine compatibility agent are applied to clinical more and more, show good effect.
Acne is the chronic inflammatory disease betiding hair follicle, sebaceous gland, is mainly in adolescence men and women.Its morbidity is relevant with the factors such as hormonal readiness, abnormal cornification of pilosebaceous duct, infected by microbes.Research shows, the increasing the weight of of the generation of propionibacterium acnes (Propionibacterium acnes, is abbreviated as P.acnes) and acne and inflammatory reaction thereof has clear and definite relation.
Propionibacterium acnes (P.acnes) also claim Propiobacterium, Propionibacterium, is a kind of anaerobism brevibacterium of Gram-positive, in the hair follicle generally residing in skin and sebaceous gland.Propionibacterium acnes plays the role of a nucleus in multiple paathogenic factors of acne, the infection such as breathing, cardiovascular, digestion, urinary system, nerve, osteoarticular system and eyes can be caused in addition, show as infective endocarditis, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and other post-operative infection complication.
Chinese medicine Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae Sanguisorba officinalis L. or Radix Sanguisorbae Sanguisorba officinalis L.var.longifolia (Bert.) that comes into leaves, Radix Sanguisorbae is herbaceos perennial, has another name called: Rhizoma cimicifugae simplicis, red silk ball, Herba Ophioglossi, little Radix Arnebiae (Radix Lithospermi), whip Fructus Jujubae beard, Matteuccia struthiopteri, spouting bleeding from anus grass.All there is distribution north and south each province of China, adaptable.Chinese medicine Radix Sanguisorbae has effect of convergence, hemostasis, antiinflammatory; Cure mainly the diseases such as gastrointestinal hemorrhage, enteritis, diarrhoea.Radix Sanguisorbae has good inhibitory action to Bacillus typhi, meningococcus, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, Salmonella etc.
Have no about Radix Sanguisorbae and extract thereof are to the research report of propionibacterium acnes inhibitory or killing effect and application.
Summary of the invention
The present invention aims to provide the medicine of the disease that a kind for the treatment of is caused by propionibacterium acnes and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract, easily produces untoward reaction over the course for the treatment of with the medicine solving Acne treatment in prior art, easily produces the technical problem of drug resistance.
To achieve these goals, according to an aspect of the present invention, provide the medicine of the disease that a kind for the treatment of is caused by propionibacterium acnes, this medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose.
Further, medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract of 0.1wt%-99.9wt%.
Further, infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and postoperative infection complication that the disease caused by propionibacterium acnes comprises acne, caused by propionibacterium acnes.
Further, Radix Sangusorbae extract is prepared from by the method for decoction extraction, circumfluence distillation, seepage pressure effects, supersound extraction, microwave extraction.
Further, Radix Sangusorbae extract is prepared from by the following method: in Radix Sanguisorbae crude drug or decoction pieces, add medical material weight 5-10 volume Extraction solvent doubly, hot reflux or supersound extraction 1-2 hour, obtain first time extracting solution; Radix Sanguisorbae crude drug or decoction pieces medical material weight 5-10 volume Extraction solvent is doubly added again in medicinal residues, hot reflux or supersound extraction 1-2 hour again, obtain second time extracting solution, merge with first time extracting solution, then carry out concentration and obtain Radix Sangusorbae extract, wherein, Extraction solvent is the ethanol of deionized water or 5%-95%.
Further, Radix Sangusorbae extract is prepared from by the following method: get Radix Sanguisorbae crude drug or decoction pieces, after expanding, adds in percolation post with water-wet, adds Extraction solvent 5%-95% ethanol to full, soaks 1-24 hour; Then add 5%-95% ethanol and carry out seepage pressure effects, keep liquid level higher than medical material, collection percolate without color to percolate, then carries out concentration and obtains Radix Sangusorbae extract.
Further, the concentration of Extraction solvent ethanol is 50%-85%.
Further, the dosage form of medicine is selected from tablet, capsule, drop pill, aerosol, gel, tincture, ointment, membrane, lotion, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, buccal tablet, suppository, lyophilized injectable powder, injection, oral liquid or paster.
According to another aspect of the present invention, the application in the medicine of the disease caused by propionibacterium acnes for the preparation for the treatment of of a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract is provided.
According to a further aspect of the invention, the application that a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract suppress in propionibacterium acnes is in vitro provided.
Apply technical scheme of the present invention, Radix Sanguisorbae or Radix Sangusorbae extract preparation is adopted to treat the medicine of the disease caused by propionibacterium acnes, Radix Sanguisorbae or Radix Sangusorbae extract belong to Chinese medicine category, its have untoward reaction over the course for the treatment of low, not easily produce the advantage such as drug resistance, further, but Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes to kill effect better.
Detailed description of the invention
It should be noted that, when not conflicting, the embodiment in the present invention and the feature in embodiment can combine mutually.The present invention is described in detail below in conjunction with embodiment.
According to a kind of typical embodiment of the present invention, the medicine of the disease that this treatment is caused by propionibacterium acnes comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose.Radix Sanguisorbae or Radix Sangusorbae extract belong to Chinese medicine category, originate natural, and make its its there is the low advantage of untoward reaction over the course for the treatment of, complicated component makes it not easily produce the advantages such as drug resistance, further, but Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes to kill effect better.Preferably, this medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract of 0.1wt%-99.9wt%.
Propionibacterium acnes is a kind of anaerobe of the Gram stain positive, is a kind of cytozoicus antibacterial, belongs to the normal flora of skin, it can the composition such as secretory protein, enzyme, lipopolysaccharide, also body can be stimulated to produce antibody, and all these can become paathogenic factor, cause various diseases.Wherein, infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis or postoperative infection complication etc. that alleged in the present invention disease caused by propionibacterium acnes can be acne, be caused by propionibacterium acnes.
" Radix Sangusorbae extract " of the present invention can be the extract adopting all methods decocting the existing Chinese medicine extraction technology such as extraction, circumfluence distillation, seepage pressure effects, supersound extraction, microwave extraction to extract Radix Sanguisorbae gained, wherein, better extracting method is circumfluence distillation.
According to a kind of typical embodiment of the present invention, Radix Sangusorbae extract is prepared from by the following method: the 5-10 volume Extraction solvent doubly adding medical material weight in Radix Sanguisorbae crude drug or decoction pieces, and hot reflux or supersound extraction 1-2 hour, release first time extracting solution; In medicinal residues, add 5-10 volume Extraction solvent doubly again, hot reflux or supersound extraction 1-2 hour again, release second time extracting solution, merge with first time extracting solution, obtain Radix Sanguisorbae extracting solution, wherein, Extraction solvent is the ethanol of deionized water or 5%-95%.By Radix Sanguisorbae extracting solution standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other Radix Sanguisorbae extracting solution carry out concentration, obtain Radix Sangusorbae extract, can be used for preparing folk prescription or compound preparation." Radix Sangusorbae extract " of the present invention, Extraction solvent can be water, 5% ~ 95% ethanol, or other organic solvent Chinese medicine extraction technology as existing in acetone, methanol etc. can all solvents, better Extraction solvent is 50%-85% ethanol.
According to the another kind of typical embodiment of the present invention, Radix Sangusorbae extract is prepared from by the following method: get Radix Sanguisorbae crude drug or decoction pieces, after expanding, adds in percolation post with water-wet, adds Extraction solvent 5%-95% ethanol to full, soaks 1-24 hour; Then slowly stream adds 5%-95% ethanol and carries out seepage pressure effects, keeps liquid level higher than medical material, collects percolate to percolate without color, obtains Radix Sanguisorbae extracting solution.This Radix Sanguisorbae extracting solution mixed standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other Radix Sanguisorbae extracting solution carry out concentration, obtain Radix Sangusorbae extract, can be used for preparing folk prescription or compound preparation.Preferably, adding the Extraction solvent ethanol for soaking in above-mentioned percolation post is the same concentration with the Extraction solvent ethanol of follow-up seepage pressure effects.Radix Sangusorbae extract of the present invention can be realized by technique scheme, but is not limited to technique scheme.
Because untoward reaction in Radix Sanguisorbae and/or Radix Sangusorbae extract therapeutic process is low, not easily produce the advantages such as drug resistance, and, Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes but to kill effect better, therefore, Radix Sanguisorbae and/or Radix Sangusorbae extract can be applied in the medicine of the disease caused by propionibacterium acnes for the preparation for the treatment of, and propionibacterium acnes aspect also can be suppressed in vitro to apply.
In the typical embodiment of one of the present invention, the medicine for the treatment of the disease caused by propionibacterium acnes comprises the Radix Sangusorbae extract for the treatment of effective dose.Alternatively, pharmaceutically acceptable carrier, excipient, adjuvant or other adjuvants is also comprised.The medicine for the treatment of the disease caused by propionibacterium acnes can be prepared according to methods known in the art.During for this object, if needed, Radix Sangusorbae extract and one or more solids or liquid pharmaceutical excipients and/or adjuvant can be combined, make and can be used as people with suitable administration form or dosage form.
Radix Sangusorbae extract of the present invention or the medicine containing it can administrations in a unit, and route of administration can be intestinal or non-bowel, as oral, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritoneum or rectum etc.Form of administration is tablet, capsule, drop pill, aerosol, gel, tincture, ointment, membrane, lotion, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, buccal tablet, suppository, lyophilized injectable powder, injection, oral liquid or paster etc. such as.Can be ordinary preparation, slow releasing preparation, immediate release dosage form or enteric dosage form and various particulate delivery system.In order to unit dosage forms for administration is made tablet, various carrier well known in the art can be widely used.Example about carrier is, such as diluent and absorbent, as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, carbamide, calcium carbonate, kaolin, microcrystalline Cellulose, aluminium silicate etc.; Wetting agent and binding agent, as water, glycerol, Polyethylene Glycol, ethanol, propanol, starch slurry, dextrin, syrup, Mel, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethyl cellulose, lac, methylcellulose, potassium phosphate, polyvinylpyrrolidone etc.; Disintegrating agent, such as dry starch, alginate, agar powder, laminaran, sodium bicarbonate and citric acid, calcium carbonate, polyoxyethylene, sorbitan fatty acid ester, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.; Disintegrate inhibitor, such as sucrose, glyceryl tristearate, cocoa butter, hydrogenation wet goods; Absorption enhancer, such as quaternary ammonium salt, sodium lauryl sulphate etc.; Lubricant, such as Pulvis Talci, silicon dioxide, corn starch, stearate, boric acid, liquid paraffin, Polyethylene Glycol etc.Tablet can also be made coated tablet further, such as sugar coated tablet, thin membrane coated tablet, ECT, or double-layer tablet and multilayer tablet.In order to administration unit is made pill, various carrier well known in the art can be widely used.Example about carrier is, such as diluent and absorbent, as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oil, polyvinylpyrrolidone, Gelucire, Kaolin, Pulvis Talci etc.; Binding agent is as arabic gum, Tragacanth, gelatin, ethanol, Mel, liquid sugar, rice paste or batter etc.; Disintegrating agent, as agar powder, dry starch, alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.In order to administration unit is made suppository, various carrier well known in the art can be widely used.Example about carrier is, the ester, gelatin, semi-synthetic glyceride etc. of such as Polyethylene Glycol, lecithin, cocoa butter, higher alcohol, higher alcohol.In order to administration unit is made capsule, effective ingredient Radix Sangusorbae extract is mixed with above-mentioned various carriers, and the mixture obtained thus is placed in hard obviously capsule or soft capsule.Also effective ingredient Radix Sangusorbae extract can be made microcapsule, be suspended in aqueous medium and form suspensoid, also can load in hard capsule or make injection application.In order to administration unit is made injection preparation, as solution, Emulsion, lyophilized injectable powder and suspensoid, all diluent that this area is conventional can be used, such as, the isooctadecanol of water, ethanol, Polyethylene Glycol, 1,3-PD, ethoxylation, polyoxygenated isooctadecanol, Polyoxyethylene Sorbitol Fatty Acid Esters etc.In addition, in order to prepare isotonic injection, appropriate sodium chloride, glucose or glycerol can be added in injection preparation, in addition, conventional cosolvent, buffer agent, pH adjusting agent etc. can also be added.
In addition, as needs, also coloring agent, antiseptic, spice, correctives, sweeting agent or other material can be added in pharmaceutical preparation.
The dosage of Radix Sangusorbae extract of the present invention depends on many factors, such as, will prevent or the character of disease therapy and the order of severity, the sex of patient or animal, age, body weight and individual reaction, route of administration and administration number of times etc.Above-mentioned dosage can single dose form or be divided into several, such as two, three or four dosage forms for administration.
When for above-mentioned treat and/or prevent or auxiliary treatment time, the Radix Sangusorbae extract treating and/or preventing effective dose can be applied in a pure form, or, can accept excipient by Radix Sangusorbae extract and one or more medicines and form pharmaceutical composition administration.Term " effective dose " refers to the dosage that can realize treating, prevent, alleviate and/or alleviating disease of the present invention or disease in experimenter.But it should be understood that the total consumption per day of medicine that the present invention contains the Radix Sangusorbae extract of effective dose must be maked decision within the scope of reliable medical judgment by attending physician.For any concrete patient, concrete treatment effective dose level must be determined according to many factors, and described factor comprises treated obstacle and the order of severity of this obstacle; Age of patient, body weight, general health situation, sex and diet; The administration time of the medicine adopted, route of administration and excretion rate; The treatment persistent period; The medicine simultaneously used; And the known similar factor of medical field.Such as, the way of this area is, the dosage of medicine, from lower than for obtaining level that required therapeutic effect requires, increases dosage, gradually until obtain required effect.
According to a further aspect in the invention, the application that a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract suppress in propionibacterium acnes is in vitro provided.Its application process comprises and the Radix Sanguisorbae of effective dose and/or Radix Sangusorbae extract being contacted with the material carrying propionibacterium acnes in vitro, as smeared Radix Sanguisorbae and/or the Radix Sangusorbae extract of effective dose at the skin surface suffering from acne.
Further illustrate the present invention below in conjunction with specific experiment, but be not limited to the present invention.
Radix Sangusorbae extract of the present invention is tested propionibacterium acnes inhibitory action
Bacteriostatic experiment 1
Experiment material:
1, strain and source: (P.a numbers propionibacterium acnes Propionibacterium acnes: 10312), Chinese industrial Microbiological Culture Collection administrative center.
2, culture medium: THIOGLYCOLLIC ACID salt broth, is purchased from National Institute for Food and Drugs Control.
3, Radix Sanguisorbae medical material: Radix Sanguisorbae decoction pieces, purchased from Beijing pharmacy of Tongrentang.
4, Maxwell opacity tube: 0.5 Maxwell standard opacity tube, is equivalent to 1.5 × 10
8cFU/mL, is purchased from Nuo Taian Science and Technology Ltd. in Beijing.
Minimal inhibitory concentration (minimal inhibitory concentration, MIC) assay method:
Double dilution method, detection by quantitative Radix Sanguisorbae is to the minimal inhibitory concentration (MIC) of propionibacterium acnes.
Experimental technique:
1, Radix Sanguisorbae extracting solution preparation (circumfluence distillation): accurately take 10 parts of each 10.0g of Radix Sanguisorbae pharmaceutical decocting piece, be placed in 150ml ground conical flask respectively, respectively add deionized water, 5% ethanol, 20% ethanol, 30% ethanol, 40% ethanol, 50% ethanol, 70% ethanol, 85% ethanol, 90% ethanol, each 100ml of 95% ethanol, put on electric hot plate and add condensation reflux unit, circumfluence distillation 1.0 hours, leaches extracting solution; Medicinal residues add each 80ml of former solvent again, and circumfluence distillation 1.0 hours, leaches extracting solution, and extracted twice liquid merges, and cooling mixing is extracting solution stock solution, measures extracting liquid volume.Getting a certain amount of extraction stock solution, with former Extraction solvent, each extracting solution crude drug concentration being all configured to 50mg/ml, for measuring minimal inhibitory concentration.
2, pastille culture medium preparation: medicinal liquid (crude drug 50mg/ml) is configured to every ml containing crude drug amount 5mg, 2.5mg, 1.25mg, 0.625mg, 0.312mg, 0.156mg, 0.078mg, 0.039mg, 0.020mg totally 9 drug level (label 1-9 manages) with THIOGLYCOLLIC ACID salt broth respectively, control tube label 10: for not adding the Growth positive control tube of medicinal liquid.Subpackage two groups, A group is pipe total amount 4.8ml (adding bacterium test group) often, and B group is pipe total amount 5.0ml (not adding bacterium matched group) often, autoclaving sterilizing.
3, bacteriostatic experiment:
(1) bacterium solution preparation is tested: get and activate the good propionibacterium acnes bacterium liquid of Zengjing Granule, centrifugal with sterile centrifugation tube, bacterial sediment physiological saline solution is diluted to 0.5 Maxwell than turbid unit, namely about 10
8cFU/ml.
(2) Accelerated Life bacterium liquid: get pastille culture medium (No. 1-10, A deck label pipe) after sterilizing, with Sterile pipette Accelerated Life bacterium liquid 0.20ml, shake up.
(3) cultivate: A group and B group put into 37 DEG C of Anaerobic culturel 3-5 days simultaneously.
(4) contrast: strain contrast is the culture medium Accelerated Life bacterium (No. 10th, A group pipe) of not drug containing, and drug control is the medicinal liquid (No. 1-10, B group pipe) of not Accelerated Life bacterium.
(5) result judges: with the identical diluted concentration of B group not Accelerated Life bacterium medicinal liquid as contrast, turbidity is index, and each pipe of naked-eye observation A group has asepsis growth, judge minimal inhibitory concentration (MIC).
Experimental result: B group is all not long bacterium of Accelerated Life bacterium not, does the comparison of same acute drug turbidity, A group Accelerated Life bacterium and the comparison of B group.The results are shown in Table 1-table 10, minimal inhibitory concentration the results are shown in Table 11 and table 12.
Table 1 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 2 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 3 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 4 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium; "-+" represent that long bacterium amount is few.
Table 5 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium; "-+" represent that long bacterium amount is few.
Table 6 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 7 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 8 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium; .
Table 9 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 10 Accelerated Life bacterium sample detection result
Note: "-" represents not have long bacterium; "+" represents long bacterium.
Table 11 water bath reflux method detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
| Extraction solvent | Water | 5% ethanol | 20% ethanol | 30% ethanol | 40% ethanol |
| MIC (crude drug mg/ml) | 1.25 | 1.25 | 1.25 | 1.25 | 0.625 |
Table 12 water bath reflux method detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
| Extraction solvent | 50% ethanol | 70% ethanol | 85% ethanol | 90% ethanol | 95% ethanol |
| MIC (crude drug mg/ml) | 0.312 | 0.312 | 0.312 | 0.625 | 0.625 |
Bacteriostatic experiment 2: except Radix Sanguisorbae extracting method, all the other methods are with bacteriostatic experiment 1.
Supersound extraction: get Radix Sanguisorbae pharmaceutical decocting piece, pulverize, accurately take 8 parts of each 10.0g, be placed in 150ml ground conical flask respectively, respectively add that deionized water, 5% ethanol, 25% ethanol, 45% ethanol, 50% ethanol, 65% ethanol are each, 85% ethanol, each 80ml of 95% ethanol, tool plug, supersound extraction 1.0 hours, leaches extracting solution; Medicinal residues add each 70ml of former solvent again, then supersound extraction 1.0 hours, leach extracting solution, and extracted twice liquid merges, and mixing is extracting solution stock solution, measure extracting liquid volume.Getting a certain amount of extraction stock solution, with former Extraction solvent, extracting solution crude drug concentration being all configured to 50mg/ml, for measuring minimal inhibitory concentration.Measurement result is in table 13.
Table 13 ultrasonic extraction detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
As can be seen from the data of table 13, when Extraction solvent is 50%-85% ethanol, the fungistatic effect of its extract is best.
Bacteriostatic experiment 3: except Radix Sanguisorbae extracting method, all the other methods are with bacteriostatic experiment 1.
Seepage pressure effects: get Radix Sanguisorbae pharmaceutical decocting piece, pulverize, accurately take 8 parts of each 20.0g, first use deionized water moistening, make moistening after medical material hold agglomerating, decontrol after loose.Then the medicated powder after moistening is poured in percolator equably, avoid degree of tightness irregular; On medicated powder, lid layer filter paper, presses bottle cap, in case medicated powder is routed up, yield is extracted in impact; Percolator lower end piston is opened, and adds Extraction solvent deionized water, 5% ethanol, 35% ethanol, 50% ethanol, 55% ethanol, 75% ethanol, 85% ethanol, 95% ethanol respectively to full, discharges bubble; Piston is closed 4 hours, soak medicated powder, then open percolation; Keep liquid level higher than medicated powder, slowly percolation, to percolate very slight color.Collect percolate, mixing, surveying record percolate cumulative volume, for extracting stock solution.Get a certain amount of extraction stock solution, with former Extraction solvent, extracting solution crude drug concentration is all configured to 50mg/ml, for measuring minimal inhibitory concentration, measurement result is in table 14.
Table 14 permeating extraction detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
Preparation method and the beneficial effect of Radix Sangusorbae extract of the present invention is further illustrated below in conjunction with embodiment.
Embodiment 1
Get Radix Sanguisorbae crude drug decoction pieces, add the 10 volumes deionized water doubly of medical material weight, circumfluence distillation 1 hour, release first time extracting solution, medicinal residues add 7.5 volumes deionized water doubly again, circumfluence distillation 1 hour again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 1.25mg/ml.
Embodiment 2
Get Radix Sanguisorbae crude drug decoction pieces, add the 9 volumes deionized water doubly of medical material weight, circumfluence distillation 1.5 hours, release first time extracting solution, medicinal residues add 8 volumes deionized water doubly again, circumfluence distillation 1 hour again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 1.25mg/ml.
Embodiment 3
Get Radix Sanguisorbae crude drug decoction pieces, add the 8 volumes deionized water doubly of medical material weight, supersound extraction 2 hours, release first time extracting solution, medicinal residues add 6 volumes deionized water doubly again, supersound extraction 1 hour again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 1.25mg/ml.
Embodiment 4
Get Radix Sanguisorbae crude drug decoction pieces, add 9 volumes, 50% ethanol doubly of medical material weight, circumfluence distillation 2 hours, release first time extracting solution, medicinal residues add 5 volumes, 50% ethanol doubly again, circumfluence distillation 1.5 hours again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 5
Get Radix Sanguisorbae crude drug decoction pieces, add 8 volumes, 60% ethanol doubly of medical material weight, circumfluence distillation 2 hours, release first time extracting solution, medicinal residues add 6 volumes, 60% ethanol doubly again, circumfluence distillation 2 hours again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 6
Get Radix Sanguisorbae crude drug decoction pieces, add 7 volumes, 70% ethanol doubly of medical material weight, circumfluence distillation 1 hour, release first time extracting solution, medicinal residues add 7 volumes, 70% ethanol doubly again, circumfluence distillation 1 hour again, release second time extracting solution, merge with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 7
Get Radix Sanguisorbae crude drug, pulverize, add 8.5 volumes, 40% ethanol doubly of medical material weight, supersound extraction 2 hours, releases first time extracting solution, medicinal residues add 7.5 volumes, 40% ethanol doubly again, supersound extraction 2 hours again, releases second time extracting solution, merges with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.625mg/ml.
Embodiment 8
Get Radix Sanguisorbae crude drug, pulverize, add 8 volumes, 55% ethanol doubly of medical material weight, supersound extraction 1 hour, releases first time extracting solution, medicinal residues add 7 volumes, 55% ethanol doubly again, supersound extraction 1 hour again, releases second time extracting solution, merges with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 9
Get Radix Sanguisorbae crude drug, pulverize, add 10 volumes, 70% ethanol doubly of medical material weight, supersound extraction 2 hours, releases first time extracting solution, medicinal residues add 6.5 volumes, 70% ethanol doubly again, supersound extraction 1 hour again, releases second time extracting solution, merges with first time extracting solution, extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 10
Get Radix Sanguisorbae crude drug, pulverize, after expanding, add in percolation post (or percolator) with water-wet, add Extraction solvent 60% ethanol to full, soaks 10 hours, more slowly stream adds 50% ethanol and carries out seepage pressure effects, maintenance liquid level is higher than medical material.To percolate substantially without color, all percolates mixed standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 11
Get Radix Sanguisorbae crude drug, pulverize, after expanding, add in percolation post (or percolator) with water-wet, add Extraction solvent 70% ethanol to full, soaks 16 hours, more slowly stream adds 60% ethanol and carries out seepage pressure effects, maintenance liquid level is higher than medical material.To percolate substantially without color, all percolates mixed standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 12
Get Radix Sanguisorbae crude drug, pulverize, after expanding, add in percolation post (or percolator) with water-wet, add Extraction solvent 80% ethanol to full, soaks 20 hours, more slowly stream adds 80% ethanol and carries out seepage pressure effects, maintenance liquid level is higher than medical material.To percolate substantially without color, all percolates mixed standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 0.312mg/ml.
Embodiment 13
Get Radix Sanguisorbae crude drug decoction pieces, add medical material weight 10 volume deionized water doubly, soak 1 hour, decoct with stainless-steel pan, keep micro-and boil 1 hour, leach extracting solution.Medicinal residues are added 7 volumes deionized water doubly again, again decoct, keep micro-and boil 1 hour, leach secondary raffinate.Extracted twice liquid is merged, mixing, standardize solution or weigh quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, obtain Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detecting crude drug minimal inhibitory concentration (MIC) by the double dilution method described in bacteriostatic test of the present invention is 1.25mg/ml.
As can be seen from above-mentioned experimental result, Radix Sangusorbae extract has good inhibitory or killing effect to propionibacterium acnes.And the external material that can present better bacteriostasis usually, its bacteriostasis is in vivo also better, and this is just for the medicine of the disease preparing Acne treatment and caused by propionibacterium acnes provides a brand-new direction.And because Radix Sanguisorbae and extract thereof are Chinese medicine, it is few that it has untoward reaction, the advantage such as not easily develop immunity to drugs, the Gospel of this vast patients with acne beyond doubt.
Because Radix Sangusorbae extract has good inhibitory or killing effect to propionibacterium acnes, and Radix Sangusorbae extract simply extracts from Radix Sanguisorbae, so, but the active substance killing propionibacterium acnes must be contained in Radix Sangusorbae extract in Radix Sanguisorbae, so Radix Sanguisorbae has inhibitory or killing effect to propionibacterium acnes.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. treat a medicine for the disease caused by propionibacterium acnes, it is characterized in that, described medicine comprises the Radix Sangusorbae extract for the treatment of effective dose, and described medicine is folk prescription; Described Radix Sangusorbae extract is prepared from by the method for circumfluence distillation, seepage pressure effects, supersound extraction;
Wherein, the method for described hot reflux or supersound extraction comprises the following steps:
Add in Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes Extraction solvent doubly, hot reflux or supersound extraction 1 ?2 hours, obtain first time extracting solution;
Add again in medicinal residues described Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes described Extraction solvent doubly, again hot reflux or supersound extraction 1 ?2 hours, obtain second time extracting solution, merge with described first time extracting solution, then carry out concentration and obtain described Radix Sangusorbae extract, wherein, described Extraction solvent be 50% ?85% ethanol;
Wherein, the method for described seepage pressure effects comprises the following steps:
Get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in percolation post, add Extraction solvent 50% ?85% ethanol to full, soak 1 ?24 hours;
Then add 50% ?85% ethanol carry out seepage pressure effects, keep liquid level higher than medical material, collect percolate to described percolate without color, then carry out concentration and obtain described Radix Sangusorbae extract.
2. medicine according to claim 1, is characterized in that, described medicine comprises the Radix Sangusorbae extract of 0.1wt% ?99.9wt%.
3. medicine according to claim 1, it is characterized in that, infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and postoperative infection complication that the described disease caused by propionibacterium acnes comprises acne, caused by propionibacterium acnes.
4. according to claim 1 ?medicine according to any one of 3, it is characterized in that, the dosage form of described medicine is selected from tablet, capsule, aerosol, gel, tincture, ointment, membrane, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, suppository, lyophilized injectable powder or paster.
5. medicine according to claim 4, is characterized in that, described tablet comprises buccal tablet, and described pill comprises drop pill; Described solution comprises lotion, injection and oral liquid.
6. the application of Radix Sangusorbae extract in the medicine of the disease caused by propionibacterium acnes for the preparation for the treatment of, it is characterized in that, described Radix Sangusorbae extract is prepared from by the method for circumfluence distillation, seepage pressure effects, supersound extraction;
Wherein, the method for described hot reflux or supersound extraction comprises the following steps:
Add in Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes Extraction solvent doubly, hot reflux or supersound extraction 1 ?2 hours, obtain first time extracting solution;
Add again in medicinal residues described Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes described Extraction solvent doubly, again hot reflux or supersound extraction 1 ?2 hours, obtain second time extracting solution, merge with described first time extracting solution, then carry out concentration and obtain described Radix Sangusorbae extract, wherein, described Extraction solvent be 50% ?85% ethanol;
Wherein, the method for described seepage pressure effects comprises the following steps:
Get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in percolation post, add Extraction solvent 50% ?85% ethanol to full, soak 1 ?24 hours;
Then add 50% ?85% ethanol carry out seepage pressure effects, keep liquid level higher than medical material, collect percolate to described percolate without color, then carry out concentration and obtain described Radix Sangusorbae extract.
7. the application of Radix Sangusorbae extract in the propionibacterium acnes suppressing culture medium culturing, it is characterized in that, described Radix Sangusorbae extract is prepared from by the method for circumfluence distillation, seepage pressure effects, supersound extraction;
Wherein, the method for described hot reflux or supersound extraction comprises the following steps:
Add in Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes Extraction solvent doubly, hot reflux or supersound extraction 1 ?2 hours, obtain first time extracting solution;
Add again in medicinal residues described Radix Sanguisorbae crude drug or decoction pieces medical material weight 5 ?10 volumes described Extraction solvent doubly, again hot reflux or supersound extraction 1 ?2 hours, obtain second time extracting solution, merge with described first time extracting solution, then carry out concentration and obtain described Radix Sangusorbae extract, wherein, described Extraction solvent be 50% ?85% ethanol;
Wherein, the method for described seepage pressure effects comprises the following steps:
Get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in percolation post, add Extraction solvent 50% ?85% ethanol to full, soak 1 ?24 hours;
Then add 50% ?85% ethanol carry out seepage pressure effects, keep liquid level higher than medical material, collect percolate to described percolate without color, then carry out concentration and obtain described Radix Sangusorbae extract.
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| CN201210119647.7A CN103372076B (en) | 2012-04-20 | 2012-04-20 | Treat the medicine of disease and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract that are caused by propionibacterium acnes |
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| CN201210119647.7A CN103372076B (en) | 2012-04-20 | 2012-04-20 | Treat the medicine of disease and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract that are caused by propionibacterium acnes |
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| CN104707015A (en) * | 2015-04-07 | 2015-06-17 | 薛青 | Medicament for operating room sterilization |
| KR102133074B1 (en) * | 2017-06-30 | 2020-07-10 | 배재대학교 산학협력단 | A cosmetic composition containing cold water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| KR102132514B1 (en) * | 2017-06-30 | 2020-08-05 | 배재대학교 산학협력단 | A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| CN108078879B (en) * | 2017-12-11 | 2019-12-24 | 华南农业大学 | Melia azedarach-lily extract for preventing and treating propionibacterium acnes and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1051506A (en) * | 1990-10-08 | 1991-05-22 | 刘墨森 | Preparation method of novel sanguisorba officinalis preparation |
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| CN1052156C (en) * | 1993-04-14 | 2000-05-10 | 刘墨森 | Medicine for curing acne-"Fenciling" |
| CN100560105C (en) * | 2007-02-08 | 2009-11-18 | 徐雪娟 | The Pharmaceutical composition of treatment acne |
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| CN1051506A (en) * | 1990-10-08 | 1991-05-22 | 刘墨森 | Preparation method of novel sanguisorba officinalis preparation |
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| Title |
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| 周祯祥等.复方痤疮液.《新编皮肤病验方荟萃》.广东世界图书出版公司,2003,(第1版), * |
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