CN103372076A - Medicine for treating diseases caused by propionibacterium acnes and application of sanguisorba officinalis and/or sanguisorba officinalis extract - Google Patents
Medicine for treating diseases caused by propionibacterium acnes and application of sanguisorba officinalis and/or sanguisorba officinalis extract Download PDFInfo
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Abstract
The invention discloses a medicine for treating diseases caused by propionibacterium acnes and application of sanguisorba officinalis and/or a sanguisorba officinalis extract, wherein the medicine comprises sanguisorba officinalis and/or sanguisorba officinalis extract in effective amount for treatment. With application of the technical scheme provided by the invention, sanguisorba officinalis and/or sanguisorba officinalis extract is adopted to prepare medicines for treating diseases caused by propionibacterium acnes, and sanguisorba officinalis and/or sanguisorba officinalis extract belongs to the scope of traditional Chinese medicines, has the advantages of low adverse reaction, difficulty in drug resistance and the like in the treatment process, and has better inhibiting and killing effect to propionibacterium acnes.
Description
Technical field
The present invention relates to field of medicaments, the medicine of the disease that is caused by propionibacterium acnes in particular to a kind for the treatment of and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract.
Background technology
Antibiotic use is effectively controlled many serious bacterial infection diseases, but its unreasonable use has caused the increase of Resistant strain, has reduced existing antibiotic use effectiveness.Chinese medicine is low because of its untoward reaction in treatment, be difficult for producing the advantage such as drug resistance, and is in widespread attention, and the folk prescription with antibacterial action of being made by Chinese medicine or compound preparation and Chinese medicine and western medicine compatibility agent are applied to clinical more and more, show good effect.
Acne is the chronic inflammatory disease that betides hair follicle, sebaceous gland, is mainly in the adolescence men and women.Its morbidity is relevant with factors such as hormonal readiness, abnormal cornification of pilosebaceous duct, infected by microbes.Studies show that the increasing the weight of of the generation of propionibacterium acnes (Propionibacterium acnes is abbreviated as P.acnes) and acne and inflammatory reaction thereof has clear and definite relation.
Propionibacterium acnes (P.acnes) also claim Propiobacterium, Propionibacterium, is a kind of anaerobism brevibacterium of Gram-positive, generally resides in the hair follicle and sebaceous gland of skin.Propionibacterium acnes plays the role of a nucleus in a plurality of paathogenic factors of acne, the infection such as breathing, cardiovascular, digestion, urinary system, nerve, osteoarthrosis system and eyes be can cause in addition, infective endocarditis, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and other post-operative infection complication shown as.
The Chinese medicine Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae Sanguisorba officinalis L. or the Radix Sanguisorbae Sanguisorba officinalis L.var.longifolia (Bert.) that comes into leaves, Radix Sanguisorbae is herbaceos perennial, has another name called: Rhizoma cimicifugae simplicis, red silk ball, Herba Ophioglossi, little Radix Arnebiae (Radix Lithospermi), whip Fructus Jujubae beard, Matteuccia struthiopteri, spouting bleeding from anus grass.All there is distribution each province, China north and south, and is adaptable.The Chinese medicine Radix Sanguisorbae has the effect of convergence, hemostasis, antiinflammatory; Cure mainly the diseases such as gastrointestinal hemorrhage, enteritis, diarrhoea.Radix Sanguisorbae has good inhibitory action to Bacillus typhi, meningococcus, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, Salmonella etc.
Have no relevant Radix Sanguisorbae and extract thereof to research report and the application of propionibacterium acnes inhibitory or killing effect.
Summary of the invention
The present invention aims to provide medicine, and the application of Radix Sanguisorbae and/or Radix Sangusorbae extract of the disease that a kind for the treatment of causes by propionibacterium acnes, easily produces untoward reaction in therapeutic process, easily produces chemical sproof technical problem with the medicine that solves Acne treatment in the prior art.
To achieve these goals, according to an aspect of the present invention, provide the medicine of the disease that a kind for the treatment of causes by propionibacterium acnes, this medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose.
Further, medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract of 0.1wt%-99.9wt%.
Further, infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and postoperative infection complication that the disease that is caused by propionibacterium acnes comprises acne, caused by propionibacterium acnes.
Further, Radix Sangusorbae extract is prepared from by the method that decocts extraction, hot reflux extraction, percolation extraction, supersound extraction, microwave extraction.
Further, Radix Sangusorbae extract is prepared from by the following method: add medical material weight 5-10 volume extraction solvent doubly in Radix Sanguisorbae crude drug or decoction pieces, hot reflux or supersound extraction 1-2 hour, obtain for the first time extracting solution; In medicinal residues, add again Radix Sanguisorbae crude drug or decoction pieces medical material weight 5-10 volume extraction solvent doubly, hot reflux or supersound extraction 1-2 hour again, obtain for the second time extracting solution, with the extracting solution merging first time, then carry out concentration and get Radix Sangusorbae extract, wherein, extracting solvent is the ethanol of deionized water or 5%-95%.
Further, Radix Sangusorbae extract is prepared from by the following method: get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in the percolation post, add and extract solvent 5%-95% ethanol to full, soaked 1-24 hour; Then add 5%-95% ethanol and carry out percolation and extract, keep liquid level to be higher than medical material, collect percolate to percolate without color till, then carry out concentration and get Radix Sangusorbae extract.
Further, the concentration of extraction etoh solvent is 50%-85%.
Further, the dosage form of medicine is selected from tablet, capsule, drop pill, aerosol, gel, tincture, ointment, membrane, lotion, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, buccal tablet, suppository, lyophilized injectable powder, injection, oral liquid or paster.
According to another aspect of the present invention, provide the application in the medicine of the disease that is caused by propionibacterium acnes for the preparation for the treatment of of a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract.
According to a further aspect of the invention, provide the application in the vitro inhibition propionibacterium acnes of a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract.
Use technical scheme of the present invention, adopt the preparation of Radix Sanguisorbae or Radix Sangusorbae extract to treat the medicine of the disease that is caused by propionibacterium acnes, Radix Sanguisorbae or Radix Sangusorbae extract belong to the Chinese medicine category, its have in therapeutic process untoward reaction low, be difficult for producing the advantage such as drug resistance, and, Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes press down to kill effect better.
The specific embodiment
Need to prove that in the situation of not conflicting, embodiment and the feature among the embodiment among the present invention can make up mutually.Describe the present invention in detail below in conjunction with embodiment.
A kind of typical embodiment according to the present invention, this treatment comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose by the medicine of the disease that propionibacterium acnes causes.Radix Sanguisorbae or Radix Sangusorbae extract belong to the Chinese medicine category, it is natural to originate, and make its its have a low advantage of untoward reaction in therapeutic process, complicated component makes it be difficult for producing the advantages such as drug resistance, and, Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes press down to kill effect better.Preferably, this medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract of 0.1wt%-99.9wt%.
Propionibacterium acnes is a kind of anaerobe of Gram stained positive, is a kind of cytozoicus antibacterial, belongs to the normal flora of skin, the compositions such as it can secretory protein, enzyme, lipopolysaccharide, also can stimulate body to produce antibody, all these can both become paathogenic factor, cause various diseases.Wherein, among the present invention the alleged disease that is caused by propionibacterium acnes can be acne, infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis or the postoperative infection complication etc. that are caused by propionibacterium acnes.
" Radix Sangusorbae extract " of the present invention can be the extract that adopts all methods extraction Radix Sanguisorbae gained of existing Chinese medicine extraction technology such as decocting extraction, hot reflux extraction, percolation extraction, supersound extraction, microwave extraction, wherein, better extracting method is that hot reflux is extracted.
A kind of typical embodiment according to the present invention, Radix Sangusorbae extract is prepared from by the following method: add the 5-10 volume extraction solvent doubly of medical material weight in Radix Sanguisorbae crude drug or the decoction pieces, hot reflux or supersound extraction 1-2 hour, emit for the first time extracting solution; In medicinal residues, add again 5-10 volume extraction solvent doubly, hot reflux or supersound extraction 1-2 hour again, emit for the second time extracting solution, with the extracting solution merging first time, get the Radix Sanguisorbae extracting solution, wherein, extracting solvent is the ethanol of deionized water or 5%-95%.With Radix Sanguisorbae extracting solution standardize solution or after weighing quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other Radix Sanguisorbae extracting solution carry out concentration, get Radix Sangusorbae extract, can be used for preparing folk prescription or compound preparation." Radix Sangusorbae extract " of the present invention, extracting solvent can be water, 5%～95% ethanol, or all solvents that can use of other organic solvent such as the existing Chinese medicine extraction technology such as acetone, methanol, better extraction solvent is 50%-85% ethanol.
The another kind of typical embodiment according to the present invention, Radix Sangusorbae extract is prepared from by the following method: get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in the percolation post, add and extract solvent 5%-95% ethanol to full, soaked 1-24 hour; Then slowly stream adds 5%-95% ethanol and carries out percolation and extract, and keeps liquid level to be higher than medical material, collect percolate to percolate without color till, get the Radix Sanguisorbae extracting solution.With this Radix Sanguisorbae extracting solution mixing standardize solution or after weighing quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other Radix Sanguisorbae extracting solution carry out concentration, get Radix Sangusorbae extract, can be used for preparing folk prescription or compound preparation.Preferably, the extraction etoh solvent that add to be used for soaks in the above-mentioned percolation post and the extraction etoh solvent of follow-up percolation extraction are the same concentration.Radix Sangusorbae extract of the present invention can be realized by technique scheme, but be not limited to technique scheme.
Produce the advantages such as drug resistance owing to untoward reaction in Radix Sanguisorbae and/or the Radix Sangusorbae extract therapeutic process is low, difficult, and, Radix Sanguisorbae or Radix Sangusorbae extract to propionibacterium acnes press down to kill effect better, therefore, Radix Sanguisorbae and/or Radix Sangusorbae extract can be used in the medicine of the disease that is caused by propionibacterium acnes for the preparation for the treatment of, also can use aspect the vitro inhibition propionibacterium acnes.
In a kind of typical embodiment of the present invention, treatment comprises the Radix Sangusorbae extract for the treatment of effective dose by the medicine of the disease that propionibacterium acnes causes.Alternatively, also comprise pharmaceutically acceptable carrier, excipient, adjuvant or other adjuvants.Treatment can be prepared according to methods known in the art by the medicine of the disease that propionibacterium acnes causes.When being used for this purpose, if necessary, Radix Sangusorbae extract and one or more solids or liquid medicine excipient and/or adjuvant can be combined, make and can be used as the suitable administration form of human or dosage form.
Radix Sangusorbae extract of the present invention or contain its medicine can the unit dosage form administration, route of administration can be intestinal or non-intestinal, such as oral, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritoneum or rectum etc.Form of administration such as tablet, capsule, drop pill, aerosol, gel, tincture, ointment, membrane, lotion, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, buccal tablet, suppository, lyophilized injectable powder, injection, oral liquid or paster etc.Can be ordinary preparation, slow releasing preparation, immediate release dosage form or enteric dosage form and various particulate delivery system.For the unit form of administration is made tablet, can be widely used various carrier well known in the art.Example about carrier is, for example diluent and absorbent are such as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, carbamide, calcium carbonate, kaolin, microcrystalline Cellulose, aluminium silicate etc.; Wetting agent and binding agent are such as water, glycerol, Polyethylene Glycol, ethanol, propanol, starch slurry, dextrin, syrup, Mel, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethyl cellulose, lac, methylcellulose, potassium phosphate, polyvinylpyrrolidone etc.; Disintegrating agent, such as dry starch, alginate, agar powder, laminaran, sodium bicarbonate and citric acid, calcium carbonate, polyoxyethylene, Sorbitol fatty acid ester, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.; Disintegrate inhibitor, for example sucrose, glyceryl tristearate, cocoa butter, hydrogenation wet goods; Absorption enhancer, such as quaternary ammonium salt, sodium lauryl sulphate etc.; Lubricant, such as Pulvis Talci, silicon dioxide, corn starch, stearate, boric acid, liquid paraffin, Polyethylene Glycol etc.Tablet further can also be made coated tablet, for example sugar coated tablet, thin membrane coated tablet, ECT, or double-layer tablet and multilayer tablet.For pill is made in the administration unit, can be widely used various carrier well known in the art.Example about carrier is, for example diluent and absorbent are such as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oil, polyvinylpyrrolidone, Gelucire, Kaolin, Pulvis Talci etc.; Binding agent such as arabic gum, Tragacanth, gelatin, ethanol, Mel, liquid sugar, rice paste or batter etc.; Disintegrating agent is such as agar powder, dry starch, alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc.For suppository is made in the administration unit, can be widely used various carrier well known in the art.Example about carrier is, such as the ester of Polyethylene Glycol, lecithin, cocoa butter, higher alcohol, higher alcohol, gelatin, semi-synthetic glyceride etc.For capsule is made in the administration unit, the effective ingredient Radix Sangusorbae extract is mixed with above-mentioned various carriers, and the mixture that will obtain thus places hard obviously capsule or soft capsule.Also the effective ingredient Radix Sangusorbae extract can be made microcapsule, be suspended in and form suspensoid in the aqueous medium, in the hard capsule of also can packing into or make injection and use.For injection preparation is made in the administration unit, such as solution, Emulsion, lyophilized injectable powder and suspensoid, can use this area all diluent commonly used, for example, the isooctadecanol of water, ethanol, Polyethylene Glycol, 1,3-PD, ethoxylation, the isooctadecanol of polyoxy, Polyoxyethylene Sorbitol Fatty Acid Esters etc.In addition, to ooze injection in order preparing etc., can in injection preparation, to add an amount of sodium chloride, glucose or glycerol, in addition, can also add conventional cosolvent, buffer agent, pH adjusting agent etc.
In addition, such as needs, also can in pharmaceutical preparation, add coloring agent, antiseptic, spice, correctives, sweeting agent or other material.
The dosage of Radix Sangusorbae extract of the present invention depends on many factors, for example will prevent or treat character and the order of severity of disease, the sex of patient or animal, age, body weight and individual reaction, route of administration and administration number of times etc.Above-mentioned dosage can the single dose form or be divided into several, for example two, three or four dosage form administrations.
When being used for above-mentioned treat and/or prevent or during auxiliary treatment, the Radix Sangusorbae extract that treats and/or prevents effective dose can be used with pure form, perhaps, can accept excipient by Radix Sangusorbae extract and one or more medicines and form the pharmaceutical composition administration.Term " effective dose " refers to realize treating, prevent, alleviate and/or alleviating the dosage of disease of the present invention or disease in the experimenter.But it should be understood that the total consumption per day of medicine that the present invention contains the Radix Sangusorbae extract of effective dosage must be examined the doctor and maked decision in the medical judgment scope reliably by main.For any concrete patient, the concrete horizontal fibrous root for the treatment of effective dose is decided according to many factors, and described factor comprises the order of severity of the obstacle for the treatment of and this obstacle; Patient's age, body weight, general health situation, sex and diet; The administration time of the medicine that adopts, route of administration and excretion rate; The treatment persistent period; The medicine that uses simultaneously; And the known similar factor of medical field.For example, the way of this area is, the dosage of medicine increases dosage, until obtain required effect from being lower than for obtaining the level that required therapeutic effect requires gradually.
According to a further aspect in the invention, provide the application in the vitro inhibition propionibacterium acnes of a kind of Radix Sanguisorbae and/or Radix Sangusorbae extract.Its application process comprises the Radix Sanguisorbae of effective dose and/or Radix Sangusorbae extract is contacted at external material with carrying propionibacterium acnes, as smear Radix Sanguisorbae and/or the Radix Sangusorbae extract of effective dose at the skin surface of suffering from acne.
Further specify the present invention below in conjunction with concrete experiment, but be not limited to the present invention.
Radix Sangusorbae extract of the present invention is tested the propionibacterium acnes inhibitory action
Bacteriostatic experiment 1
Experiment material:
1, strain and source: propionibacterium acnes Propionibacterium acnes (the P.a numbering: 10312), Chinese industrial microorganism fungus kind preservation administrative center.
2, culture medium: THIOGLYCOLLIC ACID salt fluid culture medium is purchased from National Institute for Food and Drugs Control.
3, Radix Sanguisorbae medical material: the Radix Sanguisorbae decoction pieces, available from Beijing pharmacy of Tongrentang.
4, Maxwell opacity tube: 0.5 Maxwell standard opacity tube is equivalent to 1.5 * 10
8CFU/mL is purchased from Nuo Taian Science and Technology Ltd. in Beijing.
Minimal inhibitory concentration (minimal inhibitory concentration, MIC) assay method:
Double dilution method, the detection by quantitative Radix Sanguisorbae is to the minimal inhibitory concentration (MIC) of propionibacterium acnes.
Experimental technique:
1, Radix Sanguisorbae extracting solution preparation (hot reflux extraction): accurately take by weighing 10 parts of each 10.0g of Radix Sanguisorbae pharmaceutical decocting piece, place respectively 150ml ground conical flask, each adds deionized water, 5% ethanol, 20% ethanol, 30% ethanol, 40% ethanol, 50% ethanol, 70% ethanol, 85% ethanol, 90% ethanol, each 100ml of 95% ethanol, put and add condensation reflux unit on the electric hot plate, hot reflux was extracted 1.0 hours, leached extracting solution; Medicinal residues add each 80ml of former solvent again, and hot reflux was extracted 1.0 hours, leached extracting solution, and extracted twice liquid merges, and the cooling mixing is extracting solution stock solution, measures extracting liquid volume.Get a certain amount of extraction stock solution, with former extraction solvent each extracting solution crude drug concentration all is configured to 50mg/ml, be used for measuring minimal inhibitory concentration.
2, pastille culture medium preparation: medicinal liquid (crude drug 50mg/ml) is configured to every ml with THIOGLYCOLLIC ACID salt fluid culture medium respectively and contains crude drug amount 5mg, 2.5mg, 1.25mg, 0.625mg, 0.312mg, 0.156mg, 0.078mg, 0.039mg, 0.020mg totally 9 drug level (label 1-9 pipe), control tube label 10: for not adding the positive growth control pipe of medicinal liquid.Two groups of packing, A organizes every pipe total amount 4.8ml (adding the bacterium test group), and B organizes every pipe total amount 5.0ml (not adding the bacterium matched group), the autoclaving sterilization.
3, bacteriostatic experiment:
(1) test bacterium solution preparation: get the activated cultured propionibacterium acnes bacterium of the bacterium liquid that increases, centrifugal with aseptic centrifuge tube, bacterial sediment is diluted to 0.5 Maxwell than turbid unit with physiological saline solution, and namely about 10
8CFU/ml.
(2) Accelerated Life bacterium liquid: get the rear pastille culture medium of sterilization (1-10 number pipe of A deck label), with aseptic pipet Accelerated Life bacterium liquid 0.20ml, shake up.
(3) cultivate: A group and B group are put into simultaneously 37 ℃ of anaerobism and were cultivated 3-5 days.
(4) contrast: strain contrast is not for containing the culture medium Accelerated Life bacterium (A organizes pipe No. 10) of medicine, and the medicine contrast is the medicinal liquid of Accelerated Life bacterium (the B group is managed for 1-10 number) not.
(5) result judges: the medicinal liquid of Accelerated Life bacterium is not as contrast to organize identical diluted concentration with B, and turbidity is index, and naked-eye observation A organizes each pipe asepsis growth, judges minimal inhibitory concentration (MIC).
Experimental result: B organizes not all long bacterium of Accelerated Life bacterium, does with the comparison of concentration medicine turbidity, and A group Accelerated Life bacterium is organized with B and compares.The results are shown in Table 1-table 10, minimal inhibitory concentration the results are shown in Table 11 and table 12.
Table 1 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 2 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 3 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 4 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression; "-+" represent that long bacterium amount is few.
Table 5 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression; "-+" represent that long bacterium amount is few.
Table 6 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 7 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 8 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression;
Table 9 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 10 Accelerated Life bacterium sample detection result
Annotate: "-" expression does not have long bacterium; The long bacterium of "+" expression.
Table 11 water bath reflux method detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
| Extract solvent | Water | 5% ethanol | 20% ethanol | 30% ethanol | 40% ethanol |
| MIC (crude drug mg/ml) | 1.25 | 1.25 | 1.25 | 1.25 | 0.625 |
Table 12 water bath reflux method detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
| Extract solvent | 50% ethanol | 70% ethanol | 85% ethanol | 90% ethanol | 95% ethanol |
| MIC (crude drug mg/ml) | 0.312 | 0.312 | 0.312 | 0.625 | 0.625 |
Bacteriostatic experiment 2: except the Radix Sanguisorbae extracting method, all the other methods are with bacteriostatic experiment 1.
Supersound extraction: get the Radix Sanguisorbae pharmaceutical decocting piece, pulverize, accurately take by weighing 8 parts of each 10.0g, place respectively 150ml ground conical flask, each add deionized water, 5% ethanol, 25% ethanol, 45% ethanol, 50% ethanol, 65% ethanol each, 85% ethanol, each 80ml of 95% ethanol, the tool plug, supersound extraction 1.0 hours leaches extracting solution; Medicinal residues add each 70ml of former solvent again, and supersound extraction is 1.0 hours again, leaches extracting solution, and extracted twice liquid merges, and mixing is extracting solution stock solution, measure extracting liquid volume.Get a certain amount of extraction stock solution, with former extraction solvent extracting solution crude drug concentration all is configured to 50mg/ml, be used for measuring minimal inhibitory concentration.Measurement result sees Table 13.
Table 13 ultrasonic extraction detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
Data by table 13 can find out that when the extraction solvent was 50%-85% ethanol, the fungistatic effect of its extract was best.
Bacteriostatic experiment 3: except the Radix Sanguisorbae extracting method, all the other methods are with bacteriostatic experiment 1.
Percolation extracts: get the Radix Sanguisorbae pharmaceutical decocting piece, pulverize, accurately take by weighing 8 parts of each 20.0g, use first the deionized water moistening, it is agglomerating that medical material after moistening is held, loose after decontroling.Then the medicated powder after moistening is poured in the percolator equably, avoided degree of tightness irregular; Lid layer filter paper presses bottle cap on the medicated powder, in case medicated powder is routed up, yield is extracted in impact; Percolator lower end piston is opened, and adds respectively to extract solvent deionized water, 5% ethanol, 35% ethanol, 50% ethanol, 55% ethanol, 75% ethanol, 85% ethanol, 95% ethanol to full, discharges bubble; Piston was closed 4 hours, soak medicated powder, open again percolation; Keep liquid level to be higher than medicated powder, percolation slowly is till the percolate very slight color.Collect percolate, mixing, surveying record percolate cumulative volume is for extracting stock solution.Get a certain amount of extraction stock solution, with former extraction solvent extracting solution crude drug concentration all is configured to 50mg/ml, be used for measuring minimal inhibitory concentration, measurement result sees Table 14.
Table 14 permeating extraction detects Radix Sanguisorbae medical material minimal inhibitory concentration MIC
Further specify preparation method and the beneficial effect of Radix Sangusorbae extract of the present invention below in conjunction with embodiment.
Embodiment 1
Get Radix Sanguisorbae crude drug decoction pieces, the 10 volumes deionized water doubly that adds medical material weight, hot reflux was extracted 1 hour, emit for the first time extracting solution, medicinal residues add 7.5 volumes deionized water doubly again, and hot reflux was extracted 1 hour again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 1.25mg/ml.
Embodiment 2
Get Radix Sanguisorbae crude drug decoction pieces, the 9 volumes deionized water doubly that adds medical material weight, hot reflux was extracted 1.5 hours, emit for the first time extracting solution, medicinal residues add 8 volumes deionized water doubly again, and hot reflux was extracted 1 hour again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 1.25mg/ml.
Embodiment 3
Get Radix Sanguisorbae crude drug decoction pieces, the 8 volumes deionized water doubly that adds medical material weight, supersound extraction 2 hours, emit for the first time extracting solution, medicinal residues add 6 volumes deionized water doubly again, and supersound extraction is 1 hour again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 1.25mg/ml.
Embodiment 4
Get Radix Sanguisorbae crude drug decoction pieces, 9 volumes, 50% ethanol doubly that adds medical material weight, hot reflux was extracted 2 hours, emit for the first time extracting solution, medicinal residues add 5 volumes, 50% ethanol doubly again, and hot reflux was extracted 1.5 hours again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 5
Get Radix Sanguisorbae crude drug decoction pieces, 8 volumes, 60% ethanol doubly that adds medical material weight, hot reflux was extracted 2 hours, emit for the first time extracting solution, medicinal residues add 6 volumes, 60% ethanol doubly again, and hot reflux was extracted 2 hours again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 6
Get Radix Sanguisorbae crude drug decoction pieces, 7 volumes, 70% ethanol doubly that adds medical material weight, hot reflux was extracted 1 hour, emit for the first time extracting solution, medicinal residues add 7 volumes, 70% ethanol doubly again, and hot reflux was extracted 1 hour again, emit for the second time extracting solution, with the first time extracting solution merge, after extracted twice liquid was merged standardize solution or weighs quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 7
Get the Radix Sanguisorbae crude drug, pulverize, 8.5 volumes, 40% ethanol doubly that adds medical material weight, supersound extraction 2 hours is emitted for the first time extracting solution, medicinal residues add 7.5 volumes, 40% ethanol doubly again, supersound extraction is 2 hours again, emits for the second time extracting solution, with the extracting solution merging first time, after extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.625mg/ml.
Embodiment 8
Get the Radix Sanguisorbae crude drug, pulverize, 8 volumes, 55% ethanol doubly that adds medical material weight, supersound extraction 1 hour is emitted for the first time extracting solution, medicinal residues add 7 volumes, 55% ethanol doubly again, supersound extraction is 1 hour again, emits for the second time extracting solution, with the extracting solution merging first time, after extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 9
Get the Radix Sanguisorbae crude drug, pulverize, 10 volumes, 70% ethanol doubly that adds medical material weight, supersound extraction 2 hours is emitted for the first time extracting solution, medicinal residues add 6.5 volumes, 70% ethanol doubly again, supersound extraction is 1 hour again, emits for the second time extracting solution, with the extracting solution merging first time, after extracted twice liquid merged standardize solution or weigh quantitatively, the sample that takes a morsel carried out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 10
Get the Radix Sanguisorbae crude drug, pulverize, after expanding with water-wet, add in the percolation post (or percolator), add and extract solvent 60% ethanol to full, soaked 10 hours, slowly stream adds 50% ethanol and carries out percolation and extract again, and the maintenance liquid level is higher than medical material.To percolate substantially without till the color, with all percolate mixing standardize solution or after weighing quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 11
Get the Radix Sanguisorbae crude drug, pulverize, after expanding with water-wet, add in the percolation post (or percolator), add and extract solvent 70% ethanol to full, soaked 16 hours, slowly stream adds 60% ethanol and carries out percolation and extract again, and the maintenance liquid level is higher than medical material.To percolate substantially without till the color, with all percolate mixing standardize solution or after weighing quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 12
Get the Radix Sanguisorbae crude drug, pulverize, after expanding with water-wet, add in the percolation post (or percolator), add and extract solvent 80% ethanol to full, soaked 20 hours, slowly stream adds 80% ethanol and carries out percolation and extract again, and the maintenance liquid level is higher than medical material.To percolate substantially without till the color, with all percolate mixing standardize solution or after weighing quantitatively, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other percolates carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 0.312mg/ml.
Embodiment 13
Get Radix Sanguisorbae crude drug decoction pieces, add medical material weight 10 volumes deionized water doubly, soaked 1 hour, decoct with stainless-steel pan, keep little and boiled 1 hour, leach extracting solution.Medicinal residues are added 7 volumes deionized water doubly again, again decoct, keep little and boiled 1 hour, leach secondary raffinate.With extracted twice liquid merge, mixing, standardize solution or weigh quantitatively after, the sample that takes a morsel carries out the quality testings such as content, extraction ratio, minimal inhibitory concentration.All the other extracting solution carry out concentration, get the Radix Sangusorbae extract concentrated solution, for the preparation of folk prescription or compound preparation.Detect crude drug minimal inhibitory concentration (MIC) by the double dilution method of describing in the bacteriostatic test of the present invention and be 1.25mg/ml.
Can be found out that by above-mentioned experimental result Radix Sangusorbae extract has good inhibitory or killing effect to propionibacterium acnes.And, the common external material that can present better bacteriostasis, its bacteriostasis in vivo is also better, the drug provision of the disease that this just causes for the preparation Acne treatment and by propionibacterium acnes a brand-new direction.And because Radix Sanguisorbae and extract thereof are Chinese medicine, it is few that it has a untoward reaction, the advantage such as be difficult for developing immunity to drugs, and this is undoubtedly the Gospel of vast patients with acne.
Because Radix Sangusorbae extract has good inhibitory or killing effect to propionibacterium acnes, and Radix Sangusorbae extract is simply to extract from Radix Sanguisorbae, so, must comprise in the Radix Sanguisorbae and press down the active substance that kills propionibacterium acnes in the Radix Sangusorbae extract, so Radix Sanguisorbae has inhibitory or killing effect to propionibacterium acnes.
The above is the preferred embodiments of the present invention only, is not limited to the present invention, and for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. the medicine of the disease that caused by propionibacterium acnes of a treatment is characterized in that described medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract for the treatment of effective dose.
2. medicine according to claim 1 is characterized in that, described medicine comprises Radix Sanguisorbae and/or the Radix Sangusorbae extract of 0.1wt%-99.9wt%.
3. medicine according to claim 1, it is characterized in that infectious heart film inflammation, thrombophlebitis, osteitis, arthritis, acute suppurative pericarditis and postoperative infection complication that the described disease that is caused by propionibacterium acnes comprises acne, caused by propionibacterium acnes.
4. medicine according to claim 1 is characterized in that, described Radix Sangusorbae extract is prepared from by the method that decocts extraction, hot reflux extraction, percolation extraction, supersound extraction, microwave extraction.
5. medicine according to claim 4, it is characterized in that, described Radix Sangusorbae extract is prepared from by the following method: add medical material weight 5-10 volume extraction solvent doubly in Radix Sanguisorbae crude drug or decoction pieces, hot reflux or supersound extraction 1-2 hour, obtain for the first time extracting solution;
In medicinal residues, add again described Radix Sanguisorbae crude drug or decoction pieces medical material weight 5-10 volume described extraction solvent doubly, hot reflux or supersound extraction 1-2 hour again, obtain for the second time extracting solution, with the described extracting solution merging first time, then carry out concentration and get described Radix Sangusorbae extract, wherein, the described extraction solvent ethanol that is deionized water or 5%-95%.
6. medicine according to claim 4 is characterized in that, described Radix Sangusorbae extract is prepared from by the following method: get Radix Sanguisorbae crude drug or decoction pieces, after expanding with water-wet, add in the percolation post, add and extract solvent 5%-95% ethanol to full, soaked 1-24 hour;
Then add 5%-95% ethanol and carry out percolation and extract, keep liquid level to be higher than medical material, collect percolate to described percolate without color till, then carry out concentration and get described Radix Sangusorbae extract.
7. according to claim 5 or 6 described medicines, it is characterized in that the concentration of described extraction etoh solvent is 50%-85%.
8. each described medicine according to claim 1-7, it is characterized in that the dosage form of described medicine is selected from tablet, capsule, drop pill, aerosol, gel, tincture, ointment, membrane, lotion, liniment, pill, powder, solution, suspensoid, Emulsion, granule, liposome, transdermal patch, buccal tablet, suppository, lyophilized injectable powder, injection, oral liquid or paster.
9. Radix Sanguisorbae and/or the Radix Sangusorbae extract application in the medicine of the disease that is caused by propionibacterium acnes for the preparation for the treatment of.
10. Radix Sanguisorbae and/or the Radix Sangusorbae extract application in the vitro inhibition propionibacterium acnes.
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| CN104707015A (en) * | 2015-04-07 | 2015-06-17 | 薛青 | Medicament for operating room sterilization |
| CN108078879A (en) * | 2017-12-11 | 2018-05-29 | 华南农业大学 | For preventing the fragrant chinaberry-Bulbus Lilii extract of the Huang of propionibacterium acnes and its application |
| KR20190003009A (en) * | 2017-06-30 | 2019-01-09 | 배재대학교 산학협력단 | A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| KR20190003010A (en) * | 2017-06-30 | 2019-01-09 | 배재대학교 산학협력단 | A cosmetic composition containing cold water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104707015A (en) * | 2015-04-07 | 2015-06-17 | 薛青 | Medicament for operating room sterilization |
| KR20190003009A (en) * | 2017-06-30 | 2019-01-09 | 배재대학교 산학협력단 | A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| KR20190003010A (en) * | 2017-06-30 | 2019-01-09 | 배재대학교 산학협력단 | A cosmetic composition containing cold water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| KR102133074B1 (en) * | 2017-06-30 | 2020-07-10 | 배재대학교 산학협력단 | A cosmetic composition containing cold water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| KR102132514B1 (en) * | 2017-06-30 | 2020-08-05 | 배재대학교 산학협력단 | A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium |
| CN108078879A (en) * | 2017-12-11 | 2018-05-29 | 华南农业大学 | For preventing the fragrant chinaberry-Bulbus Lilii extract of the Huang of propionibacterium acnes and its application |
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| CN103372076B (en) | 2015-10-14 |
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